Influence of Different Three-Dimensional Open Porous Titanium Scaffold Designs on Human Osteoblasts Behavior in Static and Dynamic Cell Investigations
Abstract
:1. Introduction
2. Materials and Methods
2.1. Generation and Fabrication of Micro-Structured Titanium Scaffolds
Basic Structure | Scaffold Dimensions | ||||||
---|---|---|---|---|---|---|---|
Scaffold Design | Strut Thickness a [µm] | Strut Cross-Sectional Area [mm2] | Pore Size b [µm] | Radius r [mm] | Height h [mm] | Surface Area [mm2] | Porosity [%] |
cubic | 700 * | 0.490 | 700 × 700 | 17.5 | 5.5 | ~12200 | ~51 |
pyramidal | 400 ** | 0.126 | 400–620 | 17 | 9.4 | ~18000 | ~76 |
diagonal | 400 ** | 0.126 | 400–1000 | 17 | 10.4 | ~20800 | ~75 |
2.2. Isolation and Cultivation of Human Primary Osteoblasts
2.3. Test Setup
2.4. Cell Biological Tests
2.5. Statistical Analysis
3. Results
3.1. Cubic Structure—Influence of the Manufacturing Method
3.2. Fabrication via Selective Laser-Melting—Comparison of Different Structures
4. Discussion
4.1. Static Cultivation
4.2. Dynamic Cultivation
5. Conclusions
Acknowledgments
Conflicts of Interest
References
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Markhoff, J.; Wieding, J.; Weissmann, V.; Pasold, J.; Jonitz-Heincke, A.; Bader, R. Influence of Different Three-Dimensional Open Porous Titanium Scaffold Designs on Human Osteoblasts Behavior in Static and Dynamic Cell Investigations. Materials 2015, 8, 5490-5507. https://doi.org/10.3390/ma8085259
Markhoff J, Wieding J, Weissmann V, Pasold J, Jonitz-Heincke A, Bader R. Influence of Different Three-Dimensional Open Porous Titanium Scaffold Designs on Human Osteoblasts Behavior in Static and Dynamic Cell Investigations. Materials. 2015; 8(8):5490-5507. https://doi.org/10.3390/ma8085259
Chicago/Turabian StyleMarkhoff, Jana, Jan Wieding, Volker Weissmann, Juliane Pasold, Anika Jonitz-Heincke, and Rainer Bader. 2015. "Influence of Different Three-Dimensional Open Porous Titanium Scaffold Designs on Human Osteoblasts Behavior in Static and Dynamic Cell Investigations" Materials 8, no. 8: 5490-5507. https://doi.org/10.3390/ma8085259