A Green, Simple and Facile Way to Synthesize Silver Nanoparticles Using Soluble Starch. pH Studies and Antimicrobial Applications

Along with the progress of nanoscience and nanotechnology came the means to synthesize nanometric scale materials. While changing their physical and chemical properties, they implicitly changed their application area. The aim of this paper was the synthesis of colloidal silver nanoparticles (Ag-NPs by ultrasonic disruption), using soluble starch as a reducing agent and further as a stabilizing agent for produced Ag-NPs. In this context, an important parameter for Ag-NPs preparation is the pH, which can determine the particle size and stability. The physical-chemical behavior of the synthesized Ag-NPs (shape, size, dispersion, electric charge) is strongly influenced by the pH value (experiment being conducted for pH values in the range between 8 and 13). The presence of a peak located at 412 nm into the UV-VIS spectra demonstrates the presence of silver nano-spheres into the produced material. In UV/VIS spectra, we observed a specific peak for yellow silver nano-spheres located at 412 nm. Samples characterization was performed by scanning electron microscopy, SEM, energy-dispersive X-ray spectroscopy, EDX, Fourier-transform infrared spectroscopy, and FT-IR. For all Ag-NP samples, we determined the zeta and observed that the Ag-NP particles obtained at higher pH and have better stability. Due to the intrinsic therapeutic properties and broad antimicrobial spectrum, silver nanoparticles have opened new horizons and new approaches for the control of different types of infections and wound healing abilities. In this context, the present study also aims to confirm the antimicrobial effect of prepared Ag-NPs against several bacterial strains (indicator and clinically isolated strains). In this way, it was confirmed that the antimicrobial activity of synthesized Ag-NPs was good against Staphylococcus aureus (ATCC 25923 and S. aureus MSSA) and Escherichia coli (ATTC 25922 and clinically isolated strain). Based on this observation, we conclude that the prepared Ag-NPs can represent an alternative or auxiliary material used for controlling important nosocomial pathogens. The fungal reference strain Candida albicans was more sensitive at Ag-NPs actions (zone of inhibition = 20 mm) compared with the clinically isolated strain (zone of inhibition = 10 mm), which emphasizes the greater resistance of fungal strains at antimicrobial agent’s action.


Introduction
Silver nanoparticles (Ag-NPs) have attracted attention over time due to their physical, optical, electrical, and chemical properties and due to their multiple applications [1][2][3][4]. The improvement of mechanical, catalytic, and biological characteristics has been exploited in obtain the proper dispersion of different materials involving physical-chemical interactions and different effects from clusters breaking and deagglomeration to chemical reactions [28].
Due to their intrinsic properties and multilayer actions, colloidal Ag-NPs have a broad spectrum of antimicrobial activity against different types of microorganisms, demonstrating a huge potential against even antibiotic-resistant microorganisms [29][30][31][32][33].
Ag-NPs are able to physically interact with the cell membrane of a broad spectrum of microorganisms (bacteria, fungi, etc.). Ag-NPs adhere and accumulate onto the surface of the microorganism's cell membrane. Starting from the structure of the cell membrane and the different interactions of Ag-NPs with the cell membranes of the microorganisms, this study highlights the antimicrobial activity of the synthesized material compared with silver ions from an AgNO 3 solution.
Our study is in concordance with the literature, showing that the new synthesized Ag-NPs don't present a considerable inhibition zone, but such materials present a broad spectrum of bactericidal, fungicidal [34,35], and antiviral effects [36]; thus, making them ideal in biomedical usages, such as wound dressings, dentistry [37], and cancer treatments [38].

Ag-NPs Synthesis and Characterization
For the synthesis of the colloidal Ag-NPs were used: soluble starch (Merck, Germany), DI water, AgNO 3 (Merck, Germany), and NaOH (Merck, Germany).
In order to gain information regarding the stability and electrical charge of silver nanoparticles, the zeta potential was determined using a Zeta-Meter System 3.0+, (Zeta_Meter, Inc., Staunton, VA, USA). Calibration of the apparatus was performed with a suspension standard of Min-U-SIL 100 mg/L (Merck, Darmstadt, Germany) in 100 mg/L NaCl solution. The measured zeta potential represents an average value of the results obtained after 10 readings for each sample. All samples were diluted in a ratio of 1:100.

Microbiological Activity
The antimicrobial activities of the Ag-NPs (synthesized at optimum pH) and Ag ions were determined in triplicate by the diffusimetric method according to the CLSI methodology (Clinical Laboratory and Standards Institute Inc., Wayne, PA, USA) [39].
The Each strain was placed on its appropriate growth media: Mueller-Hinton agar (Oxoid, Hampshire, UK) for Staphylococcus sp., Escherichia coli, Pseudomonas aeruginosa, and Enterococcus fecalis. Mueller Hinton 2 (Oxoid, Hampshire, UK) supplied with 5% sheep blood (BioMerieux) agar media was used for the strain of Streptococcus pneumoniae, and Sabouraud Gentamicin Chloramphenicol 2 (BioMerieux, Marcy l'Etoile, France) agar media for the strains of Candida albicans. A standardized inoculum corresponding to an optical density of 0.5 McFarland units was used. Sterile filter paper discs with a diameter of 6 mm (HiMedia Laboratories, Mumbai, India) impregnated with 20 µL of positive control test solution (different antibiotics) were deposited onto the surface of the inoculated media.
The plates were incubated at a temperature of 310 K for 24 h for the bacteria, respectively 310 K for 24 h plus 24 h more at 298 K for Candida albicans.

Ag-NPs Synthesis and Characterization
(a) Ag-NPs synthesis For the synthesis of the colloidal Ag-NPs, 1 g of soluble starch was dissolved into 25 mL of DI water and stirred for 30 min at 328 K. Concomitant 0.250 g of AgNO 3 was dissolved into 5 mL of DI water, and after complete dissolution of starch, AgNO 3 solution was added and left to stir for approximately 10 min. Before subjecting the reaction mass to ultra-sonication, the pH was adjusted in the range of 8-13 by adding NaOH. Finally, the obtained samples were ultra-sonicated in the dark, at 353 K, for 60 min.
(b) UV-VIS spectroscopy analysis UV-VIS characterization confirmed the reduction of silver ions to Ag-NPs. Because pH has an important role in the synthesis of colloidal Ag-NPs, in order to track the influence of pH on Ag-NP synthesis, UV-VIS spectra were performed for the samples at different pH values, in the range of 8-13, which are shown in Figure 1.
cal density of 0.5 McFarland units was used. Sterile filter paper discs with a diam mm (HiMedia Laboratories, Mumbai, India) impregnated with 20 µL of positive test solution (different antibiotics) were deposited onto the surface of the inocula dia. The plates were incubated at a temperature of 310 K for 24 h for the bacteria, tively 310 K for 24 h plus 24 h more at 298 K for Candida albicans.

Ag-NPs Synthesis and Characterization (a) Ag-NPs synthesis
For the synthesis of the colloidal Ag-NPs, 1 g of soluble starch was dissolved mL of DI water and stirred for 30 min at 328 K. Concomitant 0.250 g of AgNO3 w solved into 5 mL of DI water, and after complete dissolution of starch, AgNO3 was added and left to stir for approximately 10 min. Before subjecting the reactio to ultra-sonication, the pH was adjusted in the range of 8-13 by adding NaOH. the obtained samples were ultra-sonicated in the dark, at 353 K, for 60 min.  The absorption band in the visible range from 350 nm to 550 nm highlights t ence of Ag-NPs formed. The peak appears at different wavelengths between 403depending on the pH [40][41][42].
It can be seen that with the increase of pH, the intensity of the absorbance in which also indicates an increase in the concentration of Ag-NPs [43]. At pH < 10, The absorption band in the visible range from 350 nm to 550 nm highlights the presence of Ag-NPs formed. The peak appears at different wavelengths between 403-415 nm, depending on the pH [40][41][42].
It can be seen that with the increase of pH, the intensity of the absorbance increases, which also indicates an increase in the concentration of Ag-NPs [43]. At pH < 10, absorption bands are large and have a low height, which can be associated with an uneven particle distribution and a low concentration of Ag-NPs [44]. From the literature data, we found that the precipitation of silver ions with a formation of AgOH starts at a pH between 7 and 8 and stops at a pH around 13, while any further pH increase leads to precipitate dissolution [45]. At pH values greater than 10, the peaks from the UV-VIS spectra become much taller and sharper, which can be associated with a uniform distribution and higher concentration of Ag-NPs [12,44,46]. This observation is aligned with the data observed from SEM images. These data are in concordance with the information obtained from the EDX spectra, which confirm that the increase of pH leads to an increase in Ag-NP concentration.
From Figure 1, we observe that by increasing the pH to 12, it is possible to produce nanoparticles with higher stability, having smaller dimensions and uniform distribution. Furthermore, we show that at pH > 12, the absorption peak is sharper and more symmetrical, which can signify that the particles are smaller with uniform distribution. However, from the SEM images, it can be seen that at pH > 12, there is a tendency for particle agglomeration. Starting at pH 12.5, the absorbance remains approximately constant.
It is a fact that by solubilizing starch at higher temperatures, under stirring, starch hydrolysis with formation of glucose, and aldehyde groups oxidation with the formation of carboxylic groups, occurs. Furthermore, the increase of pH values leads to an increase of the hydroxyl groups with the formation of hydrogen bonds, facilitating the formation of Ag-NPs [1].
The generally accepted mechanism for Ag-NPs preparation is a two-step process. The first step is the reduction of Ag ions by glucose molecules formed during starch hydrolysis. In the second step, formed silver atoms act as nucleation centers, catalyzing the reduction reaction of remaining silver atoms. Subsequently, metallic clusters are formed by the association of silver atoms. Since, during the formation of such associations, the binding energy between different particles (metallic atoms or metallic ions) are involved, the superficial ions are again reduced, and the aggregation process continues until larger particles are obtained. Further, the nucleation process is stabilized by the interaction with the polymer, preventing particle coalescence and aggregation [1,47,48].
Simultaneously, with the increase of the pH, we noticed a color change from colorless to brown, along with the decrease of the particle size (data are depicted in Figure 2). much taller and sharper, which can be associated with a uniform distribution an concentration of Ag-NPs [12,44,46]. This observation is aligned with the data o from SEM images. These data are in concordance with the information obtained EDX spectra, which confirm that the increase of pH leads to an increase in Ag-NP tration.
From Figure 1, we observe that by increasing the pH to 12, it is possible to nanoparticles with higher stability, having smaller dimensions and uniform dist Furthermore, we show that at pH > 12, the absorption peak is sharper and more rical, which can signify that the particles are smaller with uniform distribution. H from the SEM images, it can be seen that at pH > 12, there is a tendency for pa glomeration. Starting at pH 12.5, the absorbance remains approximately constan It is a fact that by solubilizing starch at higher temperatures, under stirrin hydrolysis with formation of glucose, and aldehyde groups oxidation with the fo of carboxylic groups, occurs. Furthermore, the increase of pH values leads to an of the hydroxyl groups with the formation of hydrogen bonds, facilitating the fo of Ag-NPs [1].
The generally accepted mechanism for Ag-NPs preparation is a two-step pro first step is the reduction of Ag ions by glucose molecules formed during starch sis. In the second step, formed silver atoms act as nucleation centers, catalyzing th tion reaction of remaining silver atoms. Subsequently, metallic clusters are forme association of silver atoms. Since, during the formation of such associations, the energy between different particles (metallic atoms or metallic ions) are involved perficial ions are again reduced, and the aggregation process continues until larg cles are obtained. Further, the nucleation process is stabilized by the interaction polymer, preventing particle coalescence and aggregation [1,47,48].
Simultaneously, with the increase of the pH, we noticed a color change from to brown, along with the decrease of the particle size (data are depicted in Figure  According to the literature, the synthesized nanoparticles are spherical, symmetrical, and small [20,44]. From the data presented in Figure 3, the synthesized silver nanoparticles are present in an irregular shape when the reaction mass pH was lower than 11. This can be explained if we consider the lower rate of starch hydrolysis and nucleation, leading to the formation of nanoparticles with higher dimensions and irregular shapes [29]. Contrariwise, it was observed that at pH > 11, most of the nanoparticles present as regular spherical shapes, with dimensions in the range of 20-60 nm. Based on the data obtained from UV-VIS spectra and SEM images, the optimum pH for the synthesis of Ag-NPs is 12. At pH > 13, the formation of [Ag(OH)2] − species takes place, determining the modification of silver ion reduction potential as well as the destabilization of colloidal silver nanoparticles.
(d) Energy-dispersive X-ray spectroscopy analysis, EDX In order to highlight the presence of silver in the synthesized materials, energy-dispersive X-ray spectroscopy spectra were obtained ( Figure 4). The spectra show the emissions of characteristic X-rays from analyzed samples. The height of each specific peak is proportional to the quantity of the specific atom. To achieve such characteristic X-ray emissions, component atoms of samples were excited using an electron fascicle. According to the literature, the synthesized nanoparticles are spherical, symmetrical, and small [20,44]. From the data presented in Figure 3, the synthesized silver nanoparticles are present in an irregular shape when the reaction mass pH was lower than 11. This can be explained if we consider the lower rate of starch hydrolysis and nucleation, leading to the formation of nanoparticles with higher dimensions and irregular shapes [29]. Contrariwise, it was observed that at pH > 11, most of the nanoparticles present as regular spherical shapes, with dimensions in the range of 20-60 nm.
Based on the data obtained from UV-VIS spectra and SEM images, the optimum pH for the synthesis of Ag-NPs is 12. At pH > 13, the formation of [Ag(OH) 2 ] − species takes place, determining the modification of silver ion reduction potential as well as the destabilization of colloidal silver nanoparticles.
(d) Energy-dispersive X-ray spectroscopy analysis, EDX In order to highlight the presence of silver in the synthesized materials, energydispersive X-ray spectroscopy spectra were obtained ( Figure 4). The spectra show the emissions of characteristic X-rays from analyzed samples. The height of each specific peak is proportional to the quantity of the specific atom. To achieve such characteristic X-ray emissions, component atoms of samples were excited using an electron fascicle.
Regardless of the pH value, the silver peak can be observed in the EDX spectra, noting that the amount of silver increases with the pH, which also confirms the morphology shown in the SEM images [3,44,[49][50][51][52]. Regardless of the pH value, the silver peak can be observed in the EDX spectra, noting that the amount of silver increases with the pH, which also confirms the morphology shown in the SEM images [3,44,[49][50][51][52].
(e) Fourier transform infrared spectroscopy analysis, FT-IR The FT-IR spectra for the colloidal Ag-NPs and the starch solution, which only had the pH adjusted, without the addition of AgNO3, are shown in Figure 5. In both spectra, in the range of 3200-3550 cm −1 , a vibration appears specific to the O-H bond. Moreover, at the wavenumber 2121 cm −1 , a peak specific to the vibration of the aliphatic C-H bond appears. More significantly, a peak specific to the vibration of the C=O bond appears in the sample with starch and NaOH only, at the wavenumber of 1645 cm −1 .  Regardless of the pH value, the silver peak can be observed in the EDX spect ing that the amount of silver increases with the pH, which also confirms the morp shown in the SEM images [3,44,[49][50][51][52].

(e) Fourier transform infrared spectroscopy analysis, FT-IR
The FT-IR spectra for the colloidal Ag-NPs and the starch solution, which o the pH adjusted, without the addition of AgNO3, are shown in Figure 5. In both spectra, in the range of 3200-3550 cm −1 , a vibration appears specific to H bond. Moreover, at the wavenumber 2121 cm −1 , a peak specific to the vibratio aliphatic C-H bond appears. More significantly, a peak specific to the vibration of t bond appears in the sample with starch and NaOH only, at the wavenumber of 16 In both spectra, in the range of 3200-3550 cm −1 , a vibration appears specific to the O-H bond. Moreover, at the wavenumber 2121 cm −1 , a peak specific to the vibration of the aliphatic C-H bond appears. More significantly, a peak specific to the vibration of the C=O bond appears in the sample with starch and NaOH only, at the wavenumber of 1645 cm −1 .
At the wavenumber 1352 cm −1 , a peak specific to the vibration of the carboxyl group COOH appears, which is highlighted only in the Ag-NPs spectrum. This vibration is due to the binding of the Ag-NPs to the surface through the carboxyl group, and according to the literature data, this is specific to the synthesis of Ag-NPs [12,16,17,53].
(f) Zeta potential of Ag-NPs measurement For characterization of the colloidal particles, there is no satisfactory technique to evaluate their superficial charge. The usual practice is to determine the electrical potential of a particle from the diffuse layer, away from the particle surface. This plane, in which the particle moves, is called the slip plane or the shear plane. The measurement of the potential for this plane is called the zeta potential, which is an important parameter for colloidal nanoparticles. The zeta potential value is closely related to the suspension stability and to the particle surface morphology, but also to the interaction potential of the nanoparticles with the microbial cell wall membrane [54].
The zeta potentials of Ag-NPs, depending on the pH value, are presented in Table 1. From the obtained data from the zeta potential, results show that the nanoparticle charge is directly influenced by the pH value. Thus, in the range of 8-10, the zeta potential has positive values; therefore, the nanoparticles are positively charged. At the same time, the medium nanoparticle size is between 40 and 80 nm.
In the range of 11-13, the zeta potential has negative values, thus indicating that the nanoparticle charge is also negative, and the medium sizes of these nanoparticles are between 20 and 75 nm.
Solutions with a zeta potential less than −30 mV or greater than +30 mV have a higher level of colloidal stability [55]. At pH > 12, the zeta potential has a value of +30 mV, and silver nanoparticles are uniformly dispersed, as can be seen from the SEM. At pH > 13, silver nanoparticles tend to agglomerate due to the apparition of [Ag(OH) 2 ] − species into the system [45]. However, it is important that the oxidation process of carbonyl groups take place at a higher rate in order to cover the electrons needed to reduce silver ions. Increasing the pH leads to an increase in starch solubility, providing fragments with higher affinity and reduction capacity required during the synthesis of stable Ag-NPs colloids. Soluble starch macromolecules are composed of branched amylopectin groups. By increasing the pH, these macromolecules break into glucose fragments, which have the ability to reduce the silver ions [1].
Standard reduction potential for silver reduction is +800 mV (corresponding to the reaction Ag + + e − → Ag(s)). At pH 7 to 8, the hydrolysis of silver ions takes place: In this case, the reduction potential has a value of +344 mV, corresponding to the following reaction [1]: Glucose oxidation is taking place at a potential 750 mV. In this case, the global reaction associated with silver nanoparticle synthesis is:

Microbiological Activity
Because a growing number of bacteria, fungi, and viruses are resistant to all or almost all currently available drugs, it is necessary to develop new antimicrobial agents with superior antimicrobial properties, to target a wide range of microorganisms [56]. A possible way to develop new antibacterial agents is represented by the production of some viruses (such as phage or phage-modified viruses) that are able to specifically infect bacteria and are used in antibacterial therapy [57,58]. The usage of Ag-NPs represents an alternative method to treat bacterial infection due to the lack of side effects. This is the reason why, in this paper, we show that the newly synthesized material, based on silver nanoparticles, has increased antimicrobial potential compared to Ag ions in the AgNO 3 solution. Thus, this new material can have application in the microbiological field. For these, we tested the effectiveness of the newly synthesized material, inoculated in bacterial and fungal cultures, to demonstrate that this newly obtained material has antimicrobial activity, both antibacterial and antifungal.
To assess the antimicrobial activity, the diameter of the inhibition zone was measured in mm using a ruler. Each test was performed in triplicate, and the results were expressed as an arithmetic mean.
In the case of reference strains used as a positive control in the testing of the synthesized material, the values obtained were compared with the interval recommended by the Clinical and Laboratory Standards Institute (CLSI) for disk diffusion tests [39].
The interval framing shows that the method was correct, and these strains are quality control strains. In the case of clinically isolated strains, we reported the obtained values, and in parallel, we specified the value from which, according to CLSI, the strains are considered sensitive [39]. Table 2 shows the obtained inhibition diameters measured on Petri dishes following microbiological tests on reference strains compared with the control test and standards from CLSI.  Table 3 shows the obtained inhibition diameters, measured on Petri dishes following microbiological tests on clinically isolated or antibiotic-resistant strains, compared with the control test and standards from CLSI. Two solutions were used: AgNO 3 solution, which represents the precursor of the synthesis, and the colloidal solution of Ag-NPs.
As can be seen, regardless of all the tested microorganism types, the inhibition diameter corresponding to the Ag-NPs solution is larger than those related to the AgNO 3 solution. It is therefore obvious that the size of the Ag-NPs has a particularly important role in the exercise of the antimicrobial function, which, in the case of the sample with pH = 12, are about 20-50 nm in size. The important role of the Ag-NPs size and the specific surface area has been highlighted in various studies [59,60].
In the case of the tests for Escherichia coli and Staphylococcus aureus, while analyzing the inhibition diameters resulted from the tests done with Ag-NPs, in both the microbiological cultures (the reference bacterial and clinically isolated strains), it was observed that they didn't differ substantially. Therefore, we can conclude that the synthesized material has significant biocidal properties. In the case of Staphylococcus aureus, we can observe that the clinically isolated strain has a similar bactericidal response compared to the reference strains because the inhibition zone diameter is 19 mm in both cases.
In the case of Escherichia coli, the clinically isolated strain has a better bactericidal response (18 mm) compared to the reference strains (16 mm).
As clinically isolated bacterial strains are normally resistant to current antibiotics, the inhibitory disc would be expected to be smaller in diameter if the clinically isolated bacteria were also resistant to the synthesized material [61].
Based on this argument, we claim that the clinically isolated strains used in our tests have a lower resistance to newly synthesized material, and therefore have potential use in the medical field.
We can also see that the newly synthesized material showed an antibacterial activity on both Gram-positive and Gram-negative bacteria, even if it is slightly lower than the bactericidal activity observed in the antibiotics used as controls. One explanation would be the interaction of silver ions with the surface of the bacterial cell wall and the enzymatic thiol groups, which seem to be the first step in the degradation of the bacterial cells [59].
In this context, we consider that the tested material can be used on a complex bacterial consortium consisting of several types of bacteria, with very good bactericidal results. However, the results suggest that the synthesis of the new Ag-NP material could be considered an innovative, simple, and viable strategy to obtain a promising antibacterial solution. This is assuming the synthesized material is non-toxic to the environment and has no adverse effects as antibiotics do after long usage.
Regarding the antifungal effect of the synthesized material, in the case of Ag-NP material used on the reference culture Candida albicans (ATCC 90028), the inhibition diameter of 20 mm is comparable to that presented in the specialized protocol CLSI 2020 for the antibiotic CASPOFUNGIN 5 µg (22 mm). This falls within the range of sensitivities of the standardized antibiotic, which proves our material to be effective regarding its antifungal properties.
In the tests on clinically isolated Candida albicans strains, the results are not as promising due to the fact that the strain has a special resistance to the action of antifungal agents due to its dimorphic structure, rapid adaptation to pH fluctuations, metabolic flexibility, and the presence of a strong nutrient-acquiring system from the environment [62].
In conclusion, fungal infections remain a very serious and common disease, for which active or more concentrated antifungal agents are needed in order to control them properly. We can not exclude that by improving the properties of this material (particle size, shape, pH, or solution concentration), the antifungal activity would be improved.

Conclusions
In conclusion, this study demonstrated a simple, clean, economical, non-toxic, and environmentally-friendly technique by which colloidal Ag-NPs can be synthesized. This method uses indirect ultra-sonication with soluble starch as a stabilizer and reducer for silver nanoparticle synthesis. The reducing agent was obtained from the hydrolysis of amylopectin chains from starch macromolecules.
We also showed the relationship between the pH values and nanoparticles size. Thereby, the chosen method demonstrated the preparation of spherical silver nanoparticles with dimensions between 20 and 80 nm.
The colloidal Ag-NPs were obtained using physico-chemical characterizations, including UV-VIS spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy analysis, and Fourier transform infrared spectrometry.
An increase in the solution pH caused important modifications in the UV-VIS spectra. Therefore, the pH is directly related to the silver nanoparticle stability. Superficial plasmonic excitations observed at the Ag-NPs surface appear at variable wavelengths between 402 and 415 nm. The highest adsorption peak was observed for the silver nanoparticles obtained at pH values between 12 and 12.5. In this case, the silver nanoparticles present the lowest size and uniform distribution.
The pH influence on the electrical charge of the particle surface (related to the stability of the colloid) was highlighted by the determination of zeta potential, observing that the increase of pH leads to zeta potential increase. For the particles obtained at pH 12, the zeta potential is higher than 30 mV, which is related to the higher stability of the colloidal system. At pH > 12 and high zeta potentials, agglomeration tendency has been observed due to the formation of [Ag(OH) 2 ] − ions.
From a structural point of view, as the nanoparticle size decreases and the surfacevolume ratio increases, which results in better adhesion of Ag-NPs to the surface of the bacterial cell wall, thus increasing the antimicrobial effect of the synthesized Ag-NPs. The increased antimicrobial effect in the case of the material with Ag-NPs compared to the AgNO 3 solution is proved by comparing the size of the inhibition zone diameters between the two samples for the same microbial strain. In all tests performed, the antimicrobial response, expressed by the corresponding inhibition diameter, is superior to the Ag-NP solution. Thus, samples with Ag-NPs show antimicrobial activity that is good (for Staphylococcus aureus) or moderate (the other tested strains), but it is always superior to that manifested by Ag + ions from the AgNO 3 solution.
The clinically isolated strains of bacteria used in our tests have a lower resistance to newly synthesized material, which is a desideratum to consider our material as having potential use in the medical field.
The results obtained from the clinically isolated strain are not as promising due to the fungus' resistance to the antifungal agents, based on its dimorphic structure, rapid adaptation to environmental pH fluctuations, and metabolic flexibility. However, the references strain of Candida albicans has a very good sensibility with our synthesized material.
The study of Ag-NP synthesis mechanisms remains a challenge because small variations in the synthesis conditions lead to modifications of size, shape, and composition of prepared nanoparticles.

Conflicts of Interest:
The authors declare no conflict of interest.