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Titanium Dioxide Thin Films Obtained by Atomic Layer Deposition Promotes Osteoblasts’ Viability and Differentiation Potential While Inhibiting Osteoclast Activity—Potential Application for Osteoporotic Bone Regeneration

1
Department of Experimental Biology, Wroclaw University of Environmental and Life Sciences, Norwida St. 27 B, PL-50375 Wroclaw, Poland
2
Institute of Physics, Polish Academy of Sciences, Aleja Lotnikow 32/46, PL-02668 Warsaw, Poland
3
International Institute of Translational Medicine, Jesionowa 11 Street, 55-124 Malin, Poland
4
Collegium Medicum, Institute of Medical Science, Cardinal Stefan Wyszynski University (UKSW), Wóycickiego 1/3, 01-938 Warsaw, Poland
*
Authors to whom correspondence should be addressed.
Materials 2020, 13(21), 4817; https://doi.org/10.3390/ma13214817
Received: 18 September 2020 / Revised: 20 October 2020 / Accepted: 26 October 2020 / Published: 28 October 2020
(This article belongs to the Special Issue Scaffolds for Bone Tissue Engineering)
Atomic layer deposition (ALD) technology has started to attract attention as an efficient method for obtaining bioactive, ultrathin oxide coatings. In this study, using ALD, we have created titanium dioxide (TiO2) layers. The coatings were characterised in terms of physicochemical and biological properties. The chemical composition of coatings, as well as thickness, roughness, wettability, was determined using XPS, XRD, XRR. Cytocompatibillity of ALD TiO2 coatings was accessed applying model of mouse pre-osteoblast cell line MC3T3-E1. The accumulation of transcripts essential for bone metabolism (both mRNA and miRNA) was determined using RT-qPCR. Obtained ALD TiO2 coatings were characterised as amorphous and homogeneous. Cytocompatibility of the layers was expressed by proper morphology and growth pattern of the osteoblasts, as well as their increased viability, proliferative and metabolic activity. Simultaneously, we observed decreased activity of osteoclasts. Obtained coatings promoted expression of Opn, Coll-1, miR-17 and miR-21 in MC3T3-E1 cells. The results are promising in terms of the potential application of TiO2 coatings obtained by ALD in the field of orthopaedics, especially in terms of metabolic- and age-related bone diseases, including osteoporosis. View Full-Text
Keywords: atomic layer deposition; titanium dioxide; ultrathin layers; oxide layers; TiO2 coating; improved viability; osteogenic properties atomic layer deposition; titanium dioxide; ultrathin layers; oxide layers; TiO2 coating; improved viability; osteogenic properties
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MDPI and ACS Style

Smieszek, A.; Seweryn, A.; Marcinkowska, K.; Sikora, M.; Lawniczak-Jablonska, K.; Witkowski, B.S.; Kuzmiuk, P.; Godlewski, M.; Marycz, K. Titanium Dioxide Thin Films Obtained by Atomic Layer Deposition Promotes Osteoblasts’ Viability and Differentiation Potential While Inhibiting Osteoclast Activity—Potential Application for Osteoporotic Bone Regeneration. Materials 2020, 13, 4817. https://doi.org/10.3390/ma13214817

AMA Style

Smieszek A, Seweryn A, Marcinkowska K, Sikora M, Lawniczak-Jablonska K, Witkowski BS, Kuzmiuk P, Godlewski M, Marycz K. Titanium Dioxide Thin Films Obtained by Atomic Layer Deposition Promotes Osteoblasts’ Viability and Differentiation Potential While Inhibiting Osteoclast Activity—Potential Application for Osteoporotic Bone Regeneration. Materials. 2020; 13(21):4817. https://doi.org/10.3390/ma13214817

Chicago/Turabian Style

Smieszek, Agnieszka, Aleksandra Seweryn, Klaudia Marcinkowska, Mateusz Sikora, Krystyna Lawniczak-Jablonska, Bartlomiej. S. Witkowski, Piotr Kuzmiuk, Marek Godlewski, and Krzysztof Marycz. 2020. "Titanium Dioxide Thin Films Obtained by Atomic Layer Deposition Promotes Osteoblasts’ Viability and Differentiation Potential While Inhibiting Osteoclast Activity—Potential Application for Osteoporotic Bone Regeneration" Materials 13, no. 21: 4817. https://doi.org/10.3390/ma13214817

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