Development of Oxidized Polyvinyl Alcohol-Based Nerve Conduits Coupled with the Ciliary Neurotrophic Factor
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Preparation of PVA and Oxidized PVA Solutions
2.3. Swelling Behavior of PVA-Based Hydrogel
2.4. Porosity Measurements
2.5. Fabrication of Nerve Guidance Conduits
2.6. Morphological Characterization
2.7. Mechanical Properties and Suture Test
2.8. In Vitro Cytotoxicity Assay
2.8.1. Cell Cultures
2.8.2. Extract Test
2.8.3. MTT Assay
2.9. In Vitro Biodegradation
2.10. Preparation of Functionalized Nerve Guidance Conduits
2.10.1. Coupling CNTF to the Nerve Guides
2.10.2. Digestion and Recovery of CNTF Tryptic Peptides
2.10.3. Ultra High-Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS) Analysis of CNTF Tryptic Peptides
2.11. Statistical Analysis
3. Results
3.1. Swelling Kinetics of PVA-Based Hydrogels
3.2. Hydrogel Porosity
3.3. Morphological Characterization of Nerve Guidance Conduits
3.4. Mechanical Properties and Suture Test
3.5. Cytotoxicity Assay
3.6. Enzymatic Degradation of Nerve Conduits
3.7. Release of CNTF from Functionalized Nerve Guides
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Nerve Guidance Conduit | Area of Extracted IonP (29–40) m/z = 663.84 z = 2+ | Bound CNTF (µg) |
---|---|---|
PVA | 251,254 ± 50,751 | 0.11 ± 0.021 |
OxPVA_KMnO4 | 645,445 ± 63,984 | 0.29 ± 0.033 |
OxPVA_Br2 | 477,030 ± 48,103 | 0.22 ± 0.029 |
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Porzionato, A.; Barbon, S.; Stocco, E.; Dalzoppo, D.; Contran, M.; De Rose, E.; Parnigotto, P.P.; Macchi, V.; Grandi, C.; De Caro, R. Development of Oxidized Polyvinyl Alcohol-Based Nerve Conduits Coupled with the Ciliary Neurotrophic Factor. Materials 2019, 12, 1996. https://doi.org/10.3390/ma12121996
Porzionato A, Barbon S, Stocco E, Dalzoppo D, Contran M, De Rose E, Parnigotto PP, Macchi V, Grandi C, De Caro R. Development of Oxidized Polyvinyl Alcohol-Based Nerve Conduits Coupled with the Ciliary Neurotrophic Factor. Materials. 2019; 12(12):1996. https://doi.org/10.3390/ma12121996
Chicago/Turabian StylePorzionato, Andrea, Silvia Barbon, Elena Stocco, Daniele Dalzoppo, Martina Contran, Enrico De Rose, Pier Paolo Parnigotto, Veronica Macchi, Claudio Grandi, and Raffaele De Caro. 2019. "Development of Oxidized Polyvinyl Alcohol-Based Nerve Conduits Coupled with the Ciliary Neurotrophic Factor" Materials 12, no. 12: 1996. https://doi.org/10.3390/ma12121996