Fabrication and Application of Carboxymethyl Cellulose-Carbon Nanotube Aerogels

In this study, composite aerogels with excellent mechanical properties were prepared by using carboxymethyl cellulose (CMC) as raw materials, with carboxylic carbon nanotubes (CNTs) as reinforcement. By controlling the mass fraction of CNTs, composite aerogels with different CNTs were prepared, and the surface morphology, specific surface area, compressive modulus, density and adsorption capacities towards different oils were studied. Compared to the pure CMC aerogel, the specific surface areas of CMC/CNTs were decreased because of the agglomeration of CNTs. However, the densities of composite aerogels were lower than pure CMC aerogel. This is because the CNTs were first dispersed in water and then added to CMC solution. The results indicated that it was easy for the low CMC initial concentration to be converted to low density aerogel. The compressive modulus was increased from 0.3 MPa of pure CMC aerogel to 0.5 MPa of 5 wt % CMC/CNTs aerogel. Meanwhile, the prepared aerogels showed promising properties as the adsorption materials. Because of the high viscosity, liquid possesses strong adhesion to the pore wall, the adsorption capacity of the CMC aerogel to the liquid increases as the viscosity of the liquid increases.


Introduction
Carboxymethyl cellulose (CMC) is obtained by carboxylation modification of cellulose. CMC is non-toxic, biodegradable, biocompatible and soluble in water. It is the most common cellulose derivative and widely used in oil and gas exploration, food industry, textile printing, tissue engineering and other fields [1][2][3][4][5][6]. Due to the high crystallinity, amphiphilic molecular chains, strong intramolecular and intermolecular hydrogen bonds, cellulose has poor solubility in common organic solvents [7,8]. This has resulted in the preparation of regenerated cellulose aerogels becoming complex and time-consuming [9]. Because of the water solubility of CMC, the solvent replacement process can be omitted when preparing CMC aerogel [10]. The fabrication efficiency of CMC aerogel is greatly improved compared to regenerated cellulose aerogel.
Due to the inhomogeneous distribution of the CMC's substituents, the influence of the Degree of Substitution (DS) on the mechanical strength and pore structure of the cellulose aerogel is still unclear. Surapolchai and Schiraldi prepared CMC aerogels with different DS value (0.22-0.36) and studied their compressive modulus and surface structures. They believed that the DS of CMC had a certain influence on the mechanical properties of aerogel, but the specific influence of DS on aerogel performance still needs more experimental data support [11].
The sol-gel reaction is a process in which the material transforms from the liquid sol phase to the solid gel phase. The sol-gel reaction is the most critical step in the formation of a 3D porous

Preparation of CMC/CNTs Aerogel
Similarly, the CMC/CNTs aerogel were prepared following the same procedures of CMC aerogel, as shown in Figure 1. The only difference was after the transparent CMC solution was prepared, the pre-formed CNTs suspensions with mass fractions of 0, 1, 3, and 5 wt % (relative to the mass of cellulose) were gradually added into the solution, stirred vigorously for 2 h, poured into a mold and allowed to stand for 24 h to form CMC/CNTs hydrogel. Then CMC/CNTs aerogel was prepared by freeze-drying method. Similarly, the CMC/CNTs aerogel were prepared following the same procedures of CMC aerogel, as shown in Figure 1. The only difference was after the transparent CMC solution was prepared, the pre-formed CNTs suspensions with mass fractions of 0, 1, 3, and 5 wt % (relative to the mass of cellulose) were gradually added into the solution, stirred vigorously for 2 h, poured into a mold and allowed to stand for 24 h to form CMC/CNTs hydrogel. Then CMC/CNTs aerogel was prepared by freeze-drying method.

Characterization
The mass (m), diameter (D), and thickness (H) of the aerogel were measured using an electronic balance (ISO9001, Beijing Sartorius Instrument Co., Ltd. Beijing, China) and a digital Vernier caliper (DL91150, Ningbo Deli Tools Co., Ltd. Ningbo, China), respectively. The volume (V) and density (ρ) of the aerogel were calculated using the mathematical calculation formulas V = π•(D/2) 2 •H and ρ = m/V, respectively.
The aerogel samples were first brittle cut in liquid nitrogen. After the surface was sprayed with gold, the surface morphology of the aerogel cross section was observed by scanning electron microscope (SEM) (Phenom XL, Fu Na Scientific Instrument Co., Ltd. Holland, Netherlands) at an accelerating voltage of 5 kV. The aerogel was cut into a cylindrical shape of 10 mm × 10 mm, and the compressive modulus of the aerogel was measured by a dynamic thermomechanical analyzer (DMA) (DMA7100, Hitachi Instruments Co., Ltd., Shanghai, China) at room temperature with a minimum compressive force of 10 mN and a maximum compressive force of 200 mN. Each group was repeatedly measured three times, and the results were averaged. Nitrogen adsorption experiment of aerogels were conducted using an automatic specific surface area and microporous physical adsorption instrument (MicrotracBEL Corp, BELSORP-max, Osaka-shi, Japan). First, the aerogel was pretreated at 150 °C and <10 Pa for 8 h to remove moisture and air adsorbed. Then, a nitrogen adsorption experiment was carried out in liquid nitrogen, and the specific surface area was calculated by software using a BET (Brunauer-Emmett-Teller) equation based on the aerogel adsorptiondesorption curve. Three commonly used organic liquids, glycerol, polyethylene glycol-200 (PEG-200) and dimethylacetamide (DMAc) were selected for the adsorption experiments. The dimensions of the dry aerogels were 10 mm (diameter) by 8 mm (height). Aerogels were weighed and then immersed in liquid (100 mL) for 10 min. Then, the aerogel was removed from the liquid, allowed to drip dry above the liquid for 30 s to remove residual liquid on the surface, and weighed again. The adsorption capacity of the aerogel was calculated using the following formula: where Ca (g/g) represents the adsorption capacity of the aerogel at 10 min and mt (g) and m0 (g) represent the masses of the aerogel after or before adsorption, respectively.

Characterization
The mass (m), diameter (D), and thickness (H) of the aerogel were measured using an electronic balance (ISO9001, Beijing Sartorius Instrument Co., Ltd. Beijing, China) and a digital Vernier caliper (DL91150, Ningbo Deli Tools Co., Ltd. Ningbo, China), respectively. The volume (V) and density (ρ) of the aerogel were calculated using the mathematical calculation formulas V = π·(D/2) 2 ·H and ρ = m/V, respectively.
The aerogel samples were first brittle cut in liquid nitrogen. After the surface was sprayed with gold, the surface morphology of the aerogel cross section was observed by scanning electron microscope (SEM) (Phenom XL, Fu Na Scientific Instrument Co., Ltd. Holland, Netherlands) at an accelerating voltage of 5 kV. The aerogel was cut into a cylindrical shape of 10 mm × 10 mm, and the compressive modulus of the aerogel was measured by a dynamic thermomechanical analyzer (DMA) (DMA7100, Hitachi Instruments Co., Ltd., Shanghai, China) at room temperature with a minimum compressive force of 10 mN and a maximum compressive force of 200 mN. Each group was repeatedly measured three times, and the results were averaged. Nitrogen adsorption experiment of aerogels were conducted using an automatic specific surface area and microporous physical adsorption instrument (MicrotracBEL Corp, BELSORP-max, Osaka-shi, Japan). First, the aerogel was pretreated at 150 • C and <10 Pa for 8 h to remove moisture and air adsorbed. Then, a nitrogen adsorption experiment was carried out in liquid nitrogen, and the specific surface area was calculated by software using a BET (Brunauer-Emmett-Teller) equation based on the aerogel adsorption-desorption curve. Three commonly used organic liquids, glycerol, polyethylene glycol-200 (PEG-200) and dimethylacetamide (DMAc) were selected for the adsorption experiments. The dimensions of the dry aerogels were 10 mm (diameter) by 8 mm (height). Aerogels were weighed and then immersed in liquid (100 mL) for 10 min. Then, the aerogel was removed from the liquid, allowed to drip dry above the liquid for 30 s to remove residual liquid on the surface, and weighed again. The adsorption capacity of the aerogel was calculated using the following formula: where C a (g/g) represents the adsorption capacity of the aerogel at 10 min and m t (g) and m 0 (g) represent the masses of the aerogel after or before adsorption, respectively.

The Influence of the Degree of Substitution (DS) Value on the Properties of CMC Aerogel
The DS value of a CMC sample reflects the average number of carboxylic acid groups per glucose unit. The influence of DS of the CMC on the aerogel structures and properties was studied. SEM is one of the most common methods to analyze the surface morphology and microporous structure of porous materials. Figure 2 shows the SEM images of CMC aerogel with different DS value (0.7, 0.9 and 1.2). It is well known that the surface morphology of the aerogel largely depends on the hydrogel drying process [9,23,24]. As shown in Figure 2, the CMC aerogels prepared by freeze drying have a network structure with large pore size and sheet interconnect. This is mainly because of the formation and growth of ice crystals during hydrogel freezing process [9]. The specific surface area is the most important performance of aerogel. The static nitrogen adsorption experiment is used to analyze the BET surface area of CMC aerogels. As summarized in Table 1, the specific surface areas of the CMC aerogel are 109.94, 106.78 and 111.26 m 2 /g for DS value of 0.7, 0.9 and 1.2, respectively. The specific surface area of aerogel mainly depends on the gel drying method (freeze drying, supercritical drying or normal temperature and atmospheric drying) and parameters (for example, freezing rate and vacuum degree), and also related to the initial cellulose concentration and modification methods [23,25]. In this work, the results show that the DS value of CMC has little effect on the surface morphology and microporous structure of the aerogel.

The Influence of the Degree of Substitution (DS) Value on the Properties of CMC Aerogel
The DS value of a CMC sample reflects the average number of carboxylic acid groups per glucose unit. The influence of DS of the CMC on the aerogel structures and properties was studied. SEM is one of the most common methods to analyze the surface morphology and microporous structure of porous materials. Figure 2 shows the SEM images of CMC aerogel with different DS value (0.7, 0.9 and 1.2). It is well known that the surface morphology of the aerogel largely depends on the hydrogel drying process [9,23,24]. As shown in Figure 2, the CMC aerogels prepared by freeze drying have a network structure with large pore size and sheet interconnect. This is mainly because of the formation and growth of ice crystals during hydrogel freezing process [9]. The specific surface area is the most important performance of aerogel. The static nitrogen adsorption experiment is used to analyze the BET surface area of CMC aerogels. As summarized in Table 1, the specific surface areas of the CMC aerogel are 109.94, 106.78 and 111.26 m 2 /g for DS value of 0.7, 0.9 and 1.2, respectively. The specific surface area of aerogel mainly depends on the gel drying method (freeze drying, supercritical drying or normal temperature and atmospheric drying) and parameters (for example, freezing rate and vacuum degree), and also related to the initial cellulose concentration and modification methods [23,25]. In this work, the results show that the DS value of CMC has little effect on the surface morphology and microporous structure of the aerogel.  The compressive modulus and densities of CMC aerogels with different DS values were shown in Figure 3. For three different aerogels, the compressive modulus ranged from 0.2 to 0.4 MPa, and the densities ranged from 0.023 to 0.024 g/cm 3 . Theoretically, the reduction in the number of hydroxyl groups in the molecular chain of CMC can weaken the intermolecular forces of the cellulose aerogel, resulting in a decrease in the mechanical strength of the aerogel. However, due to the uneven distribution of substituents on the molecular chain, it is difficult to give a direct relationship between the degree of substitution and the aerogel properties. Compared with the regenerated cellulose aerogel prepared by our previous report, the density of CMC aerogel is significantly reduced. Meanwhile, the compressive modulus is also reduced [26].
The mechanical properties of cellulose aerogels are among the important properties of aerogels, which depend on the properties and concentration of the initial cellulose, crosslinks, drying methods, aerogel density, etc. [27,28]. To further enhance the mechanical properties of the CMC aerogel, different amounts of CNTs were added into the aerogel matrix with DS of 1.2. The compressive modulus and densities of CMC aerogels with different DS values were shown in Figure 3. For three different aerogels, the compressive modulus ranged from 0.2 to 0.4 MPa, and the densities ranged from 0.023 to 0.024 g/cm 3 . Theoretically, the reduction in the number of hydroxyl groups in the molecular chain of CMC can weaken the intermolecular forces of the cellulose aerogel, resulting in a decrease in the mechanical strength of the aerogel. However, due to the uneven distribution of substituents on the molecular chain, it is difficult to give a direct relationship between the degree of substitution and the aerogel properties. Compared with the regenerated cellulose aerogel prepared by our previous report, the density of CMC aerogel is significantly reduced. Meanwhile, the compressive modulus is also reduced [26].
The mechanical properties of cellulose aerogels are among the important properties of aerogels, which depend on the properties and concentration of the initial cellulose, crosslinks, drying methods, aerogel density, etc. [27,28]. To further enhance the mechanical properties of the CMC aerogel, different amounts of CNTs were added into the aerogel matrix with DS of 1.2.

The Influence of the CNTs on the Properties of CMC Aerogel
As a result of the excellent mechanical properties and water dispensability, CNTs are ideal aerogel reinforcing material. In this paper, the 2 wt % CMC solution (DS = 1.2) was mixed with 0, 1, 3 and 5 wt % of CNTs suspensions to prepare CMC/CNTs aerogel. Figure 4 shows the SEM images of CMC/CNTs composite aerogels with different amount of CNTs. CMC can be used as a binder between the CNTs to be adsorbed on CNTs to stabilize the CNTs suspension. Different amounts of CNTs suspensions were added into the CMC (DS = 1.2) solution, and CMC/CNTs aerogel was prepared by freeze drying. Table 2 summarizes the pore size distribution of aerogels which was calculated by statistical method and BET surface areas of different CNTs/CMC aerogels.
Compared with pure CMC aerogel, the addition of CNTs suspension can reduce the CMC solution concentration. Therefore, the ability to resist the damage of ice crystal growth is weakened due to the decrease of the strength of the skeleton, and the specific surface area of the aerogel is low. However, as the amount of CNTs increases, the specific surface area of the aerogel decreases, which may be because of the agglomeration of CNTs. As a result, the addition of CNTs into the CMC matrix can reduce the pore size and surface area of the aerogel.  The density and compressive modulus of CMC/CNTs aerogel were shown in Figure 5. The density of pure CMC aerogel is 0.023g/cm 3 , and the aerogel's densities were decreased after mixing the CNTs suspension. This is mainly because the CNTs suspension diluted the CMC solution. Therefore, the aerogel density was decreased with the decreasing of the initial solution concentration. The compressive modulus of aerogel was increased from 0.3 to 0.5 MPa. The addition of CNTs

The Influence of the CNTs on the Properties of CMC Aerogel
As a result of the excellent mechanical properties and water dispensability, CNTs are ideal aerogel reinforcing material. In this paper, the 2 wt % CMC solution (DS = 1.2) was mixed with 0, 1, 3 and 5 wt % of CNTs suspensions to prepare CMC/CNTs aerogel. Figure 4 shows the SEM images of CMC/CNTs composite aerogels with different amount of CNTs. CMC can be used as a binder between the CNTs to be adsorbed on CNTs to stabilize the CNTs suspension. Different amounts of CNTs suspensions were added into the CMC (DS = 1.2) solution, and CMC/CNTs aerogel was prepared by freeze drying. Table 2 summarizes the pore size distribution of aerogels which was calculated by statistical method and BET surface areas of different CNTs/CMC aerogels.
Compared with pure CMC aerogel, the addition of CNTs suspension can reduce the CMC solution concentration. Therefore, the ability to resist the damage of ice crystal growth is weakened due to the decrease of the strength of the skeleton, and the specific surface area of the aerogel is low. However, as the amount of CNTs increases, the specific surface area of the aerogel decreases, which may be because of the agglomeration of CNTs. As a result, the addition of CNTs into the CMC matrix can reduce the pore size and surface area of the aerogel.

The Influence of the CNTs on the Properties of CMC Aerogel
As a result of the excellent mechanical properties and water dispensability, CNTs are ideal aerogel reinforcing material. In this paper, the 2 wt % CMC solution (DS = 1.2) was mixed with 0, 1, 3 and 5 wt % of CNTs suspensions to prepare CMC/CNTs aerogel. Figure 4 shows the SEM images of CMC/CNTs composite aerogels with different amount of CNTs. CMC can be used as a binder between the CNTs to be adsorbed on CNTs to stabilize the CNTs suspension. Different amounts of CNTs suspensions were added into the CMC (DS = 1.2) solution, and CMC/CNTs aerogel was prepared by freeze drying. Table 2 summarizes the pore size distribution of aerogels which was calculated by statistical method and BET surface areas of different CNTs/CMC aerogels.
Compared with pure CMC aerogel, the addition of CNTs suspension can reduce the CMC solution concentration. Therefore, the ability to resist the damage of ice crystal growth is weakened due to the decrease of the strength of the skeleton, and the specific surface area of the aerogel is low. However, as the amount of CNTs increases, the specific surface area of the aerogel decreases, which may be because of the agglomeration of CNTs. As a result, the addition of CNTs into the CMC matrix can reduce the pore size and surface area of the aerogel.  The density and compressive modulus of CMC/CNTs aerogel were shown in Figure 5. The density of pure CMC aerogel is 0.023g/cm 3 , and the aerogel's densities were decreased after mixing the CNTs suspension. This is mainly because the CNTs suspension diluted the CMC solution. Therefore, the aerogel density was decreased with the decreasing of the initial solution concentration. The compressive modulus of aerogel was increased from 0.3 to 0.5 MPa. The addition of CNTs The density and compressive modulus of CMC/CNTs aerogel were shown in Figure 5. The density of pure CMC aerogel is 0.023 g/cm 3 , and the aerogel's densities were decreased after mixing the CNTs suspension. This is mainly because the CNTs suspension diluted the CMC solution.
Therefore, the aerogel density was decreased with the decreasing of the initial solution concentration. The compressive modulus of aerogel was increased from 0.3 to 0.5 MPa. The addition of CNTs improves the compression modulus of CMC aerogel and enhances its practical application performance.
improves the compression modulus of CMC aerogel and enhances its practical application performance.

The Adsorption Performance of Cellulose Aerogels
Conventional adsorption materials, such as polypropylene fibers and activated carbon, are nonbiodegradable and difficult to recycle after use [29,30]. Cellulose aerogels have porous network structures and abundant raw materials reserves, which have great application potential in the field of adsorbent materials [31]. Three liquids with different viscosity at room temperature, DMAc (0.92 mPa•s), PEG-200 (22.3 mPa•s) and Glycerol (56 mPa•s) were selected to investigate the adsorption capacities of CMC aerogel and CMC/CNTs aerogels. Figure 6 shows that the adsorption capacities of CMC aerogel to DMAc, PEG-200 and glycerol are 32.43~36.72 g/g, 45.69~48.29 g/g and 51.67~59.05 g/g, respectively. The adsorption capacities of the aerogel increases with the increasing of the liquid viscosity. The silanized cellulose aerogel prepared by Nguyen et al. has an adsorption capacities of 4~12 g/g for toluene and vacuum pump oil [32]. The maximum adsorption capacities of microcrystalline cellulose aerogel prepared by Luo et al. to pump oil and linseed oil was 10.63 and 11.44 g/g [33]. The adsorption capacities of CMC/graphene oxide aerogel prepared by Xiang et al. to organic solvents such as methanol is 10~21 g/g [34]. As shown in Figure 7, the liquid adsorption capacities of the CMC/CNTs aerogels have a similar tendency. The effects of liquid viscosity on the adsorption properties of cellulose aerogel have two aspects. First, the liquid with lower viscosity can easily penetrate into the porous network of aerogel, and the liquid with high viscosity has a large adhesion to the pore wall of aerogel and a high liquid retention rate.

The Adsorption Performance of Cellulose Aerogels
Conventional adsorption materials, such as polypropylene fibers and activated carbon, are non-biodegradable and difficult to recycle after use [29,30]. Cellulose aerogels have porous network structures and abundant raw materials reserves, which have great application potential in the field of adsorbent materials [31]. Three liquids with different viscosity at room temperature, DMAc (0.92 mPa·s), PEG-200 (22.3 mPa·s) and Glycerol (56 mPa·s) were selected to investigate the adsorption capacities of CMC aerogel and CMC/CNTs aerogels. Figure 6 shows that the adsorption capacities of CMC aerogel to DMAc, PEG-200 and glycerol are 32.43~36.72 g/g, 45.69~48.29 g/g and 51.67~59.05 g/g, respectively. The adsorption capacities of the aerogel increases with the increasing of the liquid viscosity. The silanized cellulose aerogel prepared by Nguyen et al. has an adsorption capacities of 4~12 g/g for toluene and vacuum pump oil [32]. The maximum adsorption capacities of microcrystalline cellulose aerogel prepared by Luo et al. to pump oil and linseed oil was 10.63 and 11.44 g/g [33]. The adsorption capacities of CMC/graphene oxide aerogel prepared by Xiang et al. to organic solvents such as methanol is 10~21 g/g [34]. As shown in Figure 7, the liquid adsorption capacities of the CMC/CNTs aerogels have a similar tendency. The effects of liquid viscosity on the adsorption properties of cellulose aerogel have two aspects. First, the liquid with lower viscosity can easily penetrate into the porous network of aerogel, and the liquid with high viscosity has a large adhesion to the pore wall of aerogel and a high liquid retention rate. improves the compression modulus of CMC aerogel and enhances its practical application performance.

The Adsorption Performance of Cellulose Aerogels
Conventional adsorption materials, such as polypropylene fibers and activated carbon, are nonbiodegradable and difficult to recycle after use [29,30]. Cellulose aerogels have porous network structures and abundant raw materials reserves, which have great application potential in the field of adsorbent materials [31]. Three liquids with different viscosity at room temperature, DMAc (0.92 mPa•s), PEG-200 (22.3 mPa•s) and Glycerol (56 mPa•s) were selected to investigate the adsorption capacities of CMC aerogel and CMC/CNTs aerogels. Figure 6 shows that the adsorption capacities of CMC aerogel to DMAc, PEG-200 and glycerol are 32.43~36.72 g/g, 45.69~48.29 g/g and 51.67~59.05 g/g, respectively. The adsorption capacities of the aerogel increases with the increasing of the liquid viscosity. The silanized cellulose aerogel prepared by Nguyen et al. has an adsorption capacities of 4~12 g/g for toluene and vacuum pump oil [32]. The maximum adsorption capacities of microcrystalline cellulose aerogel prepared by Luo et al. to pump oil and linseed oil was 10.63 and 11.44 g/g [33]. The adsorption capacities of CMC/graphene oxide aerogel prepared by Xiang et al. to organic solvents such as methanol is 10~21 g/g [34]. As shown in Figure 7, the liquid adsorption capacities of the CMC/CNTs aerogels have a similar tendency. The effects of liquid viscosity on the adsorption properties of cellulose aerogel have two aspects. First, the liquid with lower viscosity can easily penetrate into the porous network of aerogel, and the liquid with high viscosity has a large adhesion to the pore wall of aerogel and a high liquid retention rate.

Conclusion
In this work, CMC aerogels with different porous structure were prepared by simple cellulose dissolution, solution mixing, and freeze-drying methods. The effects of the DS value and CNTs amount on the surface structure, compressive modulus, density, specific surface area and adsorption capacities for CMC aerogels were studied. The results show that: 1) The DS value has little effect on the surface structure, mechanical properties and density of the CMC aerogels due to the uneven distribution of the substituents.