Selective Adsorption of Pb(II) from Aqueous Solution by Triethylenetetramine-Grafted Polyacrylamide/Vermiculite

Amine groups play significant roles in polymeric composites for heavy metals removal. However, generating a composite with a large number of functional and stable amine groups based on clay is still a challenge. In this work, a new amine-functionalized adsorbent based on acid-activated vermiculite (a-Verm) was prepared by organic modification of silane coupling agent as bridge, followed by in situ polymerization of acrylamide (AM) and further grafting of triethylene tetramine (TETA). The obtained polymeric composite g-PAM/OVerm was characterized by scanning electron microscope (SEM), energy dispersive spectrometer (EDS), Fourier transform infrared (FTIR), thermal analysis (TG/DTG), X-ray photoelectron spectroscopy (XPS) and Brunauer–Emmett–Teller (BET) analyses, confirming that amine groups were successfully grafted onto the surface of Verm. The efficacy g-PAM/OVerm for removing Pb(II) was tested. The adsorption equilibrium data on g-PAM/OVerm was in good accordance with the Langmuir adsorption isotherms, and the adsorption maximal value of Pb(II) was 219.4 mg·g−1. The adsorption kinetic data fit the pseudo-second-order kinetic model well. Additionally, g-PAM/OVerm has better selectivity for Pb(II) ion in comparison with Zn(II), Cd(II) and Cu(II) ions. The present work shows that g-PAM/OVerm holds great potential for removing Pb(II) from wastewater, and provides a new and efficient method for the removal of heavy metal ions from industrial wastewater.


Introduction
In recent years, heavy metal pollution is becoming more and more serious due to the rapid development of industrial activities such as mining and metallurgy [1,2]. Among the most hazardous heavy metal ions, Pb(II) has attracted much attention due to its strong and persistent toxicity even at low concentrations [3][4][5]. Consequently, the demand for effective and economical removal methods of lead compounds from aquatic environment is growing. Removal of Pb(II) is generally carried out via membrane separation [6], chemical precipitation [7], solvent extraction [8] ion-exchange [9], and adsorption [10][11][12]. Among these techniques, adsorption has become more popular in recent years, due to its availability and lower cost in industrial applications [13,14].
Natural clay materials are capable of removing heavy metals from aquatic environments, due to their chemical and mechanical stability compared with other adsorbents [15,16]. In particular, vermiculite has become one of the most studied clay materials. As an important part of layered silicate, vermiculite has a 2:1 alum inosilicate layered structure. The 2:1 layer consists of an tetrahedral sheet of silica and two sheets of octahedral MgO 2 (OH) 4 [17,18]. Exchangeable cations located in the

Modification of Verm
The procedure to modify Verm follows the protocol as described in previous work [19,41,42]. The raw vermiculite (Verm) was acid-treated as follows: 2 g vermiculite was dispersed in a 2 M HCl solution and stirred for 12 h. The mixture was filtered and washed thoroughly with water until the filtrate reached a neutral pH. The obtained material was dried at 110 • C for 8 h and denoted as acid-activated Verm (a-Verm). In a next step, the a-Verm was organically modified as follows: 3.0 g Verm, 1.0 mL water and 3.0 mL TEPM were added to 100 mL toluene with ultrasonic agitation for 30 min. The mixture was then refluxed with electromagnetic stirring at 45 • C for 4 h. The solid was separated by centrifugation and washed with toluene, anhydrous ethanol and water to remove excess modifier and possible products of hydrolysis. The obtained sample was dried in a desiccator at 80 • C. The final 3-(triethoxysilyl)propyl methacrylate-modified acid vermiculites were abbreviated as OVerm.

Preparation of PAM/OVerm
The procedure to prepare PAM/OVerm follows the steps of previous work [33,43]. OVerm (2.0 g) was dispersed in a 250-mL three-neck flask with 100 mL of toluene solution and stirred for 10 min. To the mixture, 0.6 g AM monomer and 0.06 g AIBN initiator were added. The mixture was refluxed with stirring at 80 • C for 6 h in an oil bath under N 2 atmosphere. Finally, the resulting sample was separated by centrifugation, washed with toluene and anhydrous ethanol, and dried at 80 • C. The obtained sample was denoted as PAM/OVerm.

Preparation of g-PAM/OVerm
The procedure to prepare g-PAM/OVerm follows the steps of previous work [44]. Triethylenetetramine (TETA) was incorporated to the PAM/OVerm to introduce more -NH 2 groups onto the long chain of PAM. PAM/OVerm (1.0 g) was dispersed in a 250 mL flask containing 99 mL distilled water. The pH of the mixture was adjusted to a specified value with sodium hydroxide or hydrochloric acid solution. The mixture was stirred at a specified temperature, and 1.3 mL formaldehyde was added. After a specified time, 1.5 mL TETA was added dropwise, and the reaction continued for a period of time. Finally, the grafted PAM/OVerm was separated by centrifugation and washed with distilled water until the pH of the wash was near neutral. The product was dried at 80 • C. The obtained adsorbent materials were abbreviated as g-PAM/OVerm. A schematic of the preparation of g-PAM/Overm is presented in Scheme 1. Scheme 1. The preparation of triethylenetetramine-grafted polyacrylamide/vermiculite (g-PAM/OVerm).

Characterization
Fourier transform infrared spectroscopy (FTIR) measurements were conducted on a Nicolet is 50 instrument (Thermo Fisher Scientific, Waltham, MA, USA) at a resolution of 4 cm −1 in the region of 400-4000 cm −1 using KBr for dilution. The morphology of the sample was observed with field-emission scanning electron microscopy (FE-SEM, Zeiss Supra55VP, Oberkochen, Germany) operated at an accelerating voltage of 20 kV, after coating the samples with gold film. Thermal analysis (TG and DTG) was performed with a Netzsch STA449F3 instrument system (NETZSCH Instrument Co. Ltd., Selb, Germany) at a scan rate of 10 • C·min −1 up to 800 • C in N 2 atmosphere. N 2 isotherms were obtained at −196 • C using a surface area and pore size analyzer (NOVA 2200e, Quantachrome, Boynton Beach, FL, USA). The specific surface areas (S BET ) were calculated using the Brunauer-Emmett-Teller (BET) equation within a relative pressure range (P/P 0 ) of 0.05-0.30. The pore volume was determined from the amount of N 2 adsorbed at the highest relative pressure of P/P 0~0 .99. XPS analyses were performed using an ESCALAB 250 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) with an Al KαX-ray source (1486.6 eV of photons).

Adsorption Experiments
Adsorption experiments for Pb(II) were carried out to determine the optimum conditions for chemical modification. Pb(NO 3 ) 2 was used to prepare the Pb(II) solutions. Pb(II) adsorption was carried out in a 250 mL iodine flask by dispersing 50 mg of the adsorbent in 25 mL Pb(II) aqueous solutions (400 mg·L −1 ) at different pH values. The mixtures were shaken via a constant temperature vibration shaker at 150 rpm and 30 • C. The pH of solutions was adjusted by sodium hydroxide solution or dilute nitric acid and measured by a pH meter (Mettler Toledo 320). The adsorbent was separated by centrifugation, and the Pb(II) concentration was measured by ICP-OES (VISTA-PRO CCD Simultaneous, Mason, OH, USA). The adsorption isotherms were measured in the range of 50-800 ppm at 30 • C. The effects of contact time on Pb(II) adsorption and kinetics were studied in the range of 2-120 min.
The effects of Pb(II) adsorption by g-PAM/OVerm with variable pH, temperature and reaction time during the grafting Mannich reaction was studied to obtain the optimal conditions for chemical modification. The adsorbed amount of Pb(II) at equilibrium was calculated according to the difference in Pb(II) concentration before and after adsorption, based on the following equation: where q is the Pb(II) uptake capacity by the adsorbent (mg·g −1 ), C i is the concentration of Pb(II) in solution before adsorption (mg·L −1 ), C f is the concentration of Pb(II) in solution after adsorption (mg·L −1 ), m is the dry weight of the adsorbent (g), and V is the volume of Pb(II) solution (L).

Regeneration of the Adsorbent
After the adsorption test, the adsorbent was desorbed by placing it in 25 mL dilute hydrochloric acid (1 M) and shaking at 60 • C for 60 min. After desorption, the adsorbent and liquid phase were separated by centrifugation at 6000 rpm for 10 min. The initial and final Pb(II) concentrations were measured by ICP-OES. Continuous adsorption-desorption experiments were carried out for five cycles to determine the reusability of the g-PAM/OVerm. Figure 1 shows the effect of the initial pH of the grafting reaction solution on the adsorption capacity of g-PAM/OVerm for Pb(II). It can be seen that the Pb(II) adsorption capacity increased significantly as the pH increased, reaching a maximum value of 115.6 mg·g −1 at pH 8. When the pH value continued to increase, the adsorption capacity decreased, indicating that a suitable alkaline environment is conducive to obtain a higher grafting rate of -NH 2 groups on g-PAM/OVerm. Thus, the optimal pH of the grafting reaction was 8, and this condition was used for the rest of the batch experiments.  Figure 2 shows the effect of the grafting reaction temperature on Pb(II) adsorption. Clearly, adsorption capacity for Pb(II) on g-PAM/OVerm increased with increasing reaction temperature, reaching a maximum of 125.8 mg·g −1 at 50 • C and then remaining constant. This is because higher temperature promotes molecular locomotion and diffusion velocity of PAM molecular chains, formaldehyde and TETA molecules, resulting in more effective NH 2 -grafting. The system temperature of the grafting reaction at 50 • C was used for the rest of the batch experiments.

Duration of the Grafting Reaction
According to the previous steps, the pH of the reaction system was immobilized as 8, the grafting temperature was determined as 50 • C simultaneously. The duration of the grafting reaction was optimized. For the two-step reaction of -NH 2 grafting reaction, t 1 was the time at which the hydroxymethyl derivative appeared, and t 2 was the reaction time of the hydroxymethyl derivative and TETA. These two parameters were altered in different combinations. Figure 3 shows that the optimal reaction times (t 1 and t 2 ) for NH 2 -grafting were one hour and three hours, respectively. Increasing t 2 could improve Pb(II) removal capacity with g-PAM/OVerm within a certain range. The reaction time t 1 of one hours was sufficient for hydroxylation; thus, the grafting reaction times of one hour and three hours for t 1 and t 2 , respectively, were selected for the following experiments. According to the adsorption tests above, the optimal grafting conditions were pH at 8, grafting reaction temperature at 50 • C and the combination of grafting reaction time as 1 h and 3 h. Similar work involve pH, temperature and duration of the reaction was studied by Yijiang Zhao and Shouyong Zhou [31,43]. Figure 4 shows the FTIR spectra of raw Verm, a-Verm, OVerm, PAM/OVerm and g-PAM/OVerm. Compared with the raw Verm, the Si-O stretching band gradually shifted from the characteristic position at 1010 cm −1 , for tetrahedral, to 1081 cm −1 . The peak at 1081 cm −1 is associated with Si-O stretching vibrations for amorphous silica with a three-dimensional framework, which were created on the surface of a-Verm by the simple acid treatment [45]. Moreover, the silanolization of the acid-treated product was verified by a new peak at 956 cm −1 caused by the stretching vibration of Si-OH groups exposed on the surface of a-Verm. The spectrum of OVerm shows a new band at 956 cm −1 for a-Verm (Figure 4b), which is usually representative of the silicon hydroxyl group (Si-OH). This suggests that new silanol groups (Si-OH) appeared on the surface of raw Verm after HCl modification. Absorption bands at 2959 cm −1 and 1705 cm −1 were ascribed to -C-H stretching vibrations and -COO-stretching vibrations of TEPM [46], respectively. These peaks suggest the successful modification of a-Verm with the silane coupling agent. The new absorption bands at 1671 cm −1 and 1614 cm −1 were assigned to C=O stretching vibrations and -NH 2 bending vibrations, respectively, in the spectrum of the PAM/OVerm sample. These peaks confirmed the existence of PAM chains. Additionally, the absence of the absorption band at 1705 cm −1 was possibly due to surface coverage of PAM on OVerm. After the Mannich reaction, the peak around 1614 cm −1 in the spectrum of g-PAM/OVerm increased, indicating that triethylenetetramine was successfully grafted onto PAM through copolymerization.

SEM and EDS Analyses
The SEM images given in Figure 5 show a general view on the surface morphology of a-Verm, OVerm, PAM/OVerm and g-PAM/OVerm. From Figure 5a, Verm has a typical lamellar structure and a morphology containing fragmental shape and small particles, which is different from the g-PAM/OVerm morphology. The surface of Verm appears looser and more porous in comparison with OVerm, PAM/OVerm and g-PAM/OVerm, and no obvious protuberances were observed. With further modification and -NH 2 grafting, the protuberances become more and more obvious (Figure 5b-d), which demonstrates the successful polymerization of AM and -NH 2 grafting. Despite the appearance of more and more protuberances, the lamellar structure of the Verm samples remained unchanged, appearing relatively uniform and smooth. This supports the fact that chemical modification occurred on the surface of Verm, without destroying the basic crystal structure of Verm [45]. The SEM observations further confirmed the PAM modification and -NH 2 grafting reaction on Verm.
EDS patterns of a-Verm, OVerm, PAM/OVerm and g-PAM/OVerm are displayed in Figure 6, which were obtained from the zones indicated in the SEM figures. The EDS spectra of a-Verm showed a dominant presence of Si and O, which originated from the frame of SiO 2 . After the organic modification, a small C peak was detected in OVerm, indicating that TEPM was successfully introduced into OVerm. The presence of elemental N and the increased C peak in PAM/OVerm give strong evidences for the successful immobilization of PAM on an organically modified Verm surface immediately after polymer formation (in situ immobilization). The peak of elemental N in g-PAM/OVerm doubled in height, in comparison with that of PAM/OVerm (Figure 6d). This confirmed the successful grafting of -NH 2 onto the PAM chain. The semi-quantitative EDS results are listed in Table 1, and these results are in agreement with the FTIR measurements. Furthermore, Figure 7 shows the fundamental chemical elements existed in a-Verm, OVerm, PAM/OVerm and g-PAM/OVerm, such as Si, O, C and N element, which indicates the existence of chemical elements and the homogeneous element-distribution in the corresponding samples.

Thermal Analysis
The DTG curves of Verm samples (Figure 8b) show an endothermic peak at about 91.6 • C, implying a loss of moisture. The OVerm (TEPM-modified Verm) shows an endothermic peak at 312.4 • C, which was attributed to the decomposition of TEPM [31]. Moreover, the DTG curves of PAM/OVerm and g-PAM/OVerm also show an endothermic peak at 354 • C, largely caused by the decomposition of polyacrylamide . Moreover, TG curves (Figure 8a) show that the total mass loss of a-Verm, OVerm, PAM/OVerm and g-PAM/OVerm at 25-750 • C were 11.14%, 19.18%, 24.28% and 26.11%, respectively. The mass loss demonstrates that the number of organic groups including -C-H and -NH 2 remaining in the samples per unit mass increased as the modification and grafting reactions proceeded. In other words, the TG and DTG analysis results confirmed the presence of the PAM modification and -NH 2 grafting reaction on vermiculite.

Nitrogen Adsorption-Desorption Isotherms
Nitrogen adsorption-desorption isotherms for vermiculite samples (Figure 9a) exhibit type II isotherms with a hysteresis loop, based on the IUPAC classification [47]. This indicates the existence of a large proportion of mesopores, with pore size between 2 nm and 50 nm. The hysteresis loop was type H3, due to the slit-shaped pores or plate-like particles [48]. The isotherm type for all vermiculite samples was the same. Figure 9b shows the pore width distribution of the vermiculite samples, suggesting that the pore width of vermiculite samples are distributed in the range of mesopore and micropore with the absence of macropore. Table 2 shows surface area (S BET ), pore volume and average pore diameter (Dp,a) of Raw Verm, a-Verm, OVerm, PAM/OVerm and g-PAM/OVerm. The surface area (S BET ) increased after acid treatment. Raw Verm and a-Verm had an S BET of 27.7 and 694.2 m 2 /g, respectively. The surface areas of OVerm, PAM/OVerm and g-PAM/OVerm were 396.4 m 2 /g, 247.3 m 2 /g and 201.8 m 2 /g, respectively. S BET decreased with increasing modification and grafting reactions. The smaller S BET of functionalized Verm was a consequence of the surface coverage of the silane coupling agent and polyacrylamide, which reduced the adsorption of N 2 . The same conclusion can be obtained by the changes of pore volume.

The Effect of pH on Pb(II) Uptake
One of the main parameters affecting the adsorption process is the pH of the aqueous solution [49]. The effects of initial pH on the adsorption capacity of Pb(II) by a-Verm, PAM/OVerm and g-PAM/OVerm were studied individually, within the pH range of 2.5-5.5 (Figure 10a). Throughout the studied pH range, precipitation of Pb(II) did not occur. The adsorption capacity of Pb(II) ions increased with increasing initial pH, and maximum adsorption was achieved for an initial pH value of 5.5. Compared with a-Verm and PAM/OVerm, g-PAM/OVerm exhibited much higher adsorption capacity, which indicates that more -NH 2 groups were successfully grafted onto PAM/OVerm. In addition, Pb(II) adsorption on g-PAM/OVerm was mainly due to the chelation between Pb(II) and the -NH 2 groups. Furthermore, the amine groups were deprotonated, and the Pb(II)-amide linkage formation increased with increasing initial pH [36].
The Zeta potential of a-Verm, PAM/OVerm and g-PAM/OVerm as function of solution pH is shown in Figure 10b. With the increasing of pH vaule, the Zeta potential decreases. The Zeta potential values for the surface of a-Verm over the entire pH range from 2-8, which can be ascribed to the hydroxyl groups located at the surfaces of a-Verm [50]. Especially, positively charged surface of PAM/OVerm and g-PAM/OVerm can be observed when the pH value was lower than 4, which can be attributed the protonation of the -NH 2 groups [51]. Compared with PAM/OVerm, the Zeta potential value of g-PAM/OVerm is more positive, suggesting that more -NH 2 groups were grafted onto PAM/OVerm by the Mannich reaction. In addition, the Pb(II) adsorption sites might be occupied by a large amount of hydrogen ions when the pH value is~2, making the adsorption quantity relatively low. In addition, g-PAM/OVerm shows stronger adsorption ability for Pb(II) compared with a-Verm and PAM/OVerm, which might be due to the larger quantity of -NH 2 functional groups in g-PAM/OVerm.

Study on the Adsorption Selectivity by g-PAM/OVerm
To further verify whether g-PAM/OVerm have special adsorption selectivity for Pb(II) ions, a multi-component adsorption experiment was carried out. The initial concentrations of Zn(II), Cd(II), Pb(II) and Cu(II) are all 800 mg·L −1 . Results of selective adsorption experiments of the nanocomposites for heavy metal ions in mixed solutions are shown in Figure 11. The adsorption capacities of g-PAM/OVerm for Zn(II), Cd(II), Pb(II) and Cu(II) ions are 25.3, 37.8, 153.8 and 68.3 mg·g −1 , respectively. The adsorption capacity for Pb(II) ion is much higher than that for Zn(II), Cd(II) and Cu(II) ions, suggesting that g-PAM/OVerm has better selectivity for Pb(II) ion in comparison with Zn(II), Cd(II) and Cu(II) ions under the same condition, which can be attributed to the stronger affinity between Pb(II) ion and -NH 2 groups of g-PAM/OVerm [52,53].

g-PAM/OVerm Regeneration
The regeneration of g-PAM/OVerm for Pb(II) adsorption was performed in a batch experiment. Hydrochloric acid was used to desorb the Pb(II) from the adsorbents. Samples were kept in hydrochloric acid for 30 min in a constant temperature vibration shaker. After centrifugation, the supernatant was discarded and the g-PAM/OVerm was reused. Figure 12a reveals the Pb(II) adsorption capacity by g-PAM/OVerm during five cycles of adsorption/desorption, and Figure 12b shows the evolution of the desorption efficiencies during four-time desorption calculated from the five cycles of adsorption/desorption. The regenerated g-PAM/OVerm maintained 90% of its adsorption capacity after the fifth test cycle and 90.2% of its desorption efficiency after the fourth desorption process, which suggests that g-PAM/OVerm adsorbents are promising for the efficient adsorption of Pb(II).

Adsorption Dynamics
The effects of time on the adsorption of Pb(II) by g-PAM/OVerm are given in Figure 13a,b. The adsorption rate was very fast during the first 20 min, and the removal of Pb(II) was 120.1 mg·g −1 . After that, the adsorption rate was very slow, and the adsorption equilibrium for Pb(II) was reached within 60 min.
For the purpose of determining the reaction mechanisms and mass transfer controlling Pb(II) removal by the g-PAM/OVerm adsorbent, pseudo-second-order and pseudo-first-order kinetic equations were used to analyze the experimental data. The pseudo-first-order kinetic model can be expressed in a nonlinear form as [54,55]: where q t and q e are the Pb(II) uptake amounts (mg·g −1 ) by the adsorbent g-PAM/OVerm at time t and at equilibrium; k 1 (min −1 ) is the rate constant of the pseudo-first-order adsorption. The values of k 1 , q e and the correlation coefficient (r 2 ) were determined from the nonlinear plot of q t versus t (Figure 13a). The pseudo-second-order kinetic model can be expressed from the following equation [56,57]: Equation (3) can be converted into the nonlinear form (Equation (4)), which has the ability to predict the adsorption equilibrium and form an intuitive contrast with the pseudo-first-order kinetic model: where k 2 (g·mg −1 ·min −1 ) is the rate constant of pseudo-second-order kinetic model. The constants can be calculated from plotting (t/q t ) versus t. Figure 13b presents the plot of q t versus t for the adsorption of Pb(II) onto g-PAM/OVerm in the form of a nonlinear plot. Table 3 presents the values of k, q e and the correlation coefficient (r 2 ) of the pseudo-first-order and pseudo-second-order kinetic models. From Table 3, the correlation coefficient of the pseudo-first-order model was relatively low, showing a poor fit to the adsorption data. However, the correlation coefficient for the pseudo-second-order was more than 0.995. The calculated q e value was very close to the experimental data, according to pseudo-second-order kinetic model. The adsorption dynamics analysis suggests that the Pb(II) adsorption kinetics fit the pseudo-second-order model. Therefore, the pseudo-second-order mechanism was determined as the predominant kinetic process of Pb(II) adsorption by g-PAM/OVerm, which corresponds to chemical adsorption as the rate-controlling step [57].

Adsorption Isotherms
The effects of initial concentration and adsorption capacity on Pb(II) removal were investigated by adding 50 mg of g-PAM/OVerm into 25 mL of Pb(II) solutions, with a range of Pb(II) concentrations, for 6 h at 30 • C. Langmuir and Freundlich isotherm models (Equations (5) and (6)) were carried out to explore the Pb(II) adsorption equilibrium [58,59]: Here, q m is the maximum capacity of adsorption (mg·g −1 ), q e is the equilibrium adsorption capacity (mg·g −1 ) for Pb(II), C e is the Pb(II) concentration at equilibrium in the solution (mg·L −1 ), K L and K F are the Langmuir and Freundlich isotherm equilibrium constants (L·mg −1 ), respectively.
The results are shown in Figure 14a,b and Table 4. In general, Pb(II) adsorption by g-PAM/OVerm increased with increasing initial Pb(II) concentrations, and the correlation coefficient values suggest the Langmuir model better represents the adsorption isotherm, than the Freundlich model. This indicates that the monolayer adsorption mechanism describes the adsorption of Pb(II) because the active sites are distributed evenly on the surface of g-PAM/OVerm [60]. Based on the Langmuir equation, the theoretical maximum adsorption capacity (q m ) of Pb(II) on g-PAM/OVerm was 219.4 mg·g −1 . The results obtained from this study were found to be higher than that of many corresponding adsorbents reported in the literature ( Table 5), revealing that modified vermiculite is suitable for the removal of Pb(II) ions from aqueous solutions. Therefore, considering the economic advantage and the adsorption capacity, g-PAM/OVerm is a very potential adsorbent for Pb(II) ions.

Pb(II) Adsorption Mechanism
For the purpose of determining the adsorption mechanism, high resolution XPS spectra analyses were carried out for g-PAM/OVerm before and after adsorption of Pb(II). Figure 15 reveals the characteristic N 1s spectra of g-PAM/OVerm before and after Pb(II) adsorption. Before Pb(II) adsorption, two peaks, including one weak and one strong, were found in the N 1s spectra at the binding energy (BE) of about 401.24 and 399.54 eV, respectively (Figure 15a). The strong peak was attributed to nitrogen atoms of the -NH 2 groups in the grafted TETA. The weaker peak at 401.24 eV was attributed to nitrogen atoms of the O=C-NH 2 groups in PAM, indicating that the number of -NH 2 groups in the grafted TETA was far greater than that in PAM [33]. However, one more peak appeared at 398.96 eV in the N 1s spectra after Pb(II) adsorption (Figure 15b). The intensity of the new peak is very strong relatively, indicating that a number of nitrogen atoms existed in a more reduced state after Pb(II) adsorption [32]. This resulted from the formation of the covalent bond between -NH 2 and Pb. Thus, the density of the electron cloud for the nitrogen atom increased, decreasing the BE of the peak. Therefore, the XPS analyses supported the formation of covalent bonds between amide groups and Pb(II) as the adsorption mechanism.

Conclusions
In this work, an effective approach to functionalization of Verm for removal of Pb(II) was developed by significantly improving specific surface area and silanol groups of Verm by acid activation and further modifying Verm surface with a series of organic reactions for introducing more functional amine groups. By silane coupling agent modification, polymerization of AM and -NH 2 -grafting Mannich reaction, more nitrogen Lewis basic centers were created, thus significantly enhancing the Verm adsorption capacity for Pb(II). The amine-functionalized Verm showed much greater efficiency in Pb(II) adsorption at different pH values than unmodified Verm. Furthermore, g-PAM/OVerm has better selectivity for Pb(II) ion in comparison with Zn(II), Cd(II) and Cu(II) ions. The adsorption equilibrium data on g-PAM/OVerm were in good agreement with the Langmuir adsorption isotherms, and the adsorption maximal value of Pb(II) was 219.4 mg·g −1 . The kinetics data fit the pseudo-second-order kinetic well. The great adsorption capacity of g-PAM/OVerm might be because of the presence of strong covalent bonds between Pb(II) and the -NH 2 groups. This methodology can be expanded to activate the surfaces of various materials with different functional groups such as -COOH, -OH and -SH. It is concluded that the new amine-functionalized Verm could be used as a low cost and new effective adsorbent for removing Pb(II) from aqueous systems. The present work contributes to the development of novel cost-efficient adsorbents with great adsorption capacities and unique selectivity for different heavy metals.