Mesoporous Activated Carbon Supported Ru Catalysts to Efficiently Convert Cellulose into Sorbitol by Hydrolytic Hydrogenation

Catalysts consisting of Ru nanoparticles (1 wt%), supported on mesoporous activated carbons (ACs), were prepared and used in the one-pot hydrolytic hydrogenation of cellulose to obtain sorbitol. The carbon materials used as supports are a pristine commercial mesoporous AC (named SA), and two samples derived from it by sulfonation or oxidation treatments (named SASu and SAS, respectively). The catalysts have been thoroughly characterized regarding both surface chemistry and porosity, as well as Ru electronic state and particle size. The amount and type of surface functional groups in the carbon materials becomes modified as a result of the Ru incorporation process, while a high mesopore volume is preserved upon functionalization and Ru incorporation. The prepared catalysts have shown to be very active, with cellulose conversion close to 50% and selectivity to sorbitol above 75%. The support functionalization does not lead to an improvement of the catalysts’ behavior and, in fact, the Ru/SA catalyst is the most effective one, with about 50% yield to sorbitol, and a very low generation of by-products.


Introduction
Concerns on the climate change explain the increasing interest of researchers in low carbon fuels and sustainable energy, which has boosted the investigation on the production of biofuels and chemicals from renewable feedstocks like biomass [1,2]. One of the most attractive approaches is based on the use of non-food biomass, whose conversion into valuable chemicals has recently become the object of study for many researchers dealing with biorefinery processes [1,[3][4][5], and with related reactions such as hydrolysis, pyrolysis, fermentation, dehydration, hydrogenation, etc. A big effort is being made in this field in order to convert cellulose into sugar alcohols. Among these compounds, sorbitol is attractive because of its large number of applications: in pharmacies, food, cosmetics, and as an alternative for biofuel production [6][7][8]. This polyol can be obtained by two consecutive reactions: hydrolysis of cellulose to produce glucose, followed by glucose hydrogenation to sorbitol, meaning that the process requires two different catalytic functions. In fact, it is usually carried out with catalytic systems consisting of mineral liquid acids (like H 2 SO 4 or HCl) as hydrolysis catalysts, and supported metals with activity in hydrogenation reactions [9,10]. However, liquid acids are considered not to be green options, because of their corrosive properties and because they cannot be reused. Stable acidic solids are an interesting alternative because they can be easily recovered from the liquid media [11,12], diminishing its contamination, and they can, potentially, be reused. Because of that, they can be considered promising catalysts that could replace liquid acids to make the processes greener.

Catalysts Preparation and Characterization
The commercial powder activated carbon SA-30 from MeadWestvaco (USA) was used for this study, with abbreviated name SA. It was submitted to the following chemical treatments: A-1 M H 2 SO 4 , and B-(NH 4 ) 2 S 2 O 8 saturated solution in 1 M H 2 SO 4 . In both treatments, the 1 g carbon/10 mL solution mixture was stirred for 24 h at room temperature. Afterwards, the solid was recovered by filtration and washed several times with distilled water (up to the total elimination of sulfates in the filtrate (determined by BaCl 2 testing)). The samples resulting from these treatments are named as SASu and SAS, respectively.
Ru nanoparticles were supported on the carbon samples (SA, SASu and SAS) as follows: each degasified carbon sample (150 • C, 4 h, vacuum) was put in contact with a RuCl 3 aqueous solution (1 g carbon/10 mL solution) of the appropriate concentration to obtain 1 wt% Ru loading, and kept under stirring for 14 h at room temperature. Afterwards, the mixture was stirred in an ultrasound bath for 3 h. Finally, the solvent was removed at 60 • C for 10 h, and then it was dried at 110 • C for 24 h. Before being used in a catalytic activity test, the Ru containing samples, named Ru/SA, Ru/SAS and Ru/SASu, were submitted to a reduction treatment under H 2 flow (80 mL/min), at 250 • C for 4 h.
Preparation conditions have been selected after previous, still unpublished, works. The textural properties of the original and Ru containing carbon materials were determined by N 2 adsorption-desorption at −196 • C (Autosorb-6B, Quantachrome). The specific surface area (S BET ) and the total micropore volume (V micro ) were determined from N 2 adsorption data by applying the Brunauer-Emmett-Teller (BET) and the Dubinin-Radushkevich (DR) equations, respectively. To estimate the mesopore volume (V meso ), the difference of the volume of N 2 adsorbed as liquid at P/P 0 = 0.9 and P/P 0 = 0.2 was calculated [25,26].
The surface chemistry of the prepared catalysts was studied by temperature programmed desorption (TPD) experiments in the following conditions: the sample (9-12 mg) was heated at 20 • C/min in He flow (100 mL/min) up to 900 • C. The equipment used was a thermobalance (TA-SDT Q600) coupled to a mass spectrometer (Thermostar, Balzers), allowing the simultaneous record of weight loss and the analysis of evolved gases (CO 2 , CO, and H 2 O) during the experiment.
Transmission electron microscopy (TEM, JEOL JEM-2010), with the Infinity Analyze software for image analysis, was used to analyze the size and distribution of the supported Ru particles.
X-ray photoelectron spectroscopy (XPS, VG Microtech Multilab ESCA-3000 spectrometer) was used to characterize the surface chemical composition and the electronic state of Ru in the prepared catalysts.

Cellulose Hydrolytic Hydrogenation
The commercial Avicel microcrystalline cellulose (99%, Sigma Aldrich) was ball-milled (agate balls/cellulose weight ratio of 3500 rpm, with reverse rotation every 60 min, for 7 h). Catalytic tests were carried out in a 50 mL stainless steel Parr reactor (Model 4792) lined with a Teflon container, and equipped with a manometer and a thermocouple (see Figure S1 (S accounts for Supplementary Material)), as follows: 500 mg milled cellulose, 125 mg catalyst and 25 mL distilled water, together with a magnetic rod, were introduced in the reactor. After closing the reactor, it was purged several times to remove air, and then, it was filled with H 2 and heated, under stirring, to reach the reaction conditions of 50 bar and 190 • C. The reaction time was 3 h in all catalytic tests. Reaction conditions have been selected after previous, still unpublished, works. To determine the reaction progress, the solid and liquid phases were separated by filtration, after cooling down, and then, the liquid phase was analyzed by high performance liquid chromatography (HPLC, 1200 infinity Agilent Technologies Hi-Plex Ca (Duo), 300 × 6.5 mm. An example of the obtained spectra is shown in Figure S2. The remaining solid (catalyst and unreacted cellulose) was dried and weighted to calculate the cellulose conversion.
The products yield was calculated from HPLC results, as indicated in Equation (1): Conversion was calculated as shown in Equation (2): Moreover, selectivity was calculated in terms of conversion and yield as follows (Equation (3)):

Textural Properties
The −196 • C N 2 adsorption-desorption isotherms are presented in Figure S3. They are type IV according to the IUPAC classification [27]. The relatively high adsorption at low relative pressure and the steep slope indicates that these materials contain significant volumes of both micro and mesopores.
The textural parameters calculated from the isotherms data are shown in Table 1. Carbon SA presents high surface area and pore volume, with a similar proportion of micro and mesopore volumes. Treatment A (1 M H 2 SO 4 ), that leads to sample SASu, results in a slight modification of the porous structure of the SA carbon, while treatment B ((NH 4 ) 2 S 2 O 8 saturated solution in 1 M H 2 SO 4 ), leading to sample SAS, produces a significant decrease of the adsorption capacity of the original carbon material. Such an effect can be due either to the destruction of pores, or to some blockage of the porosity by the abundant surface oxygen groups. This was previously reported for other carbon materials submitted to a similar treatment [28]. [c] mesopore volume estimated by the difference of the volume of N 2 adsorbed as liquid at P/P 0 = 0.9 and P/P 0 = 0.2.
The incorporation of Ru produces only a slight decrease of the surface area and porosity of SA, SASu and SAS carbons, indicating that the supported Ru nanoparticles produce almost no blockage of the carbon porosity. Thus, comparing the Ru-containing catalysts, it can be observed that Ru/SA and Ru/SASu show a similar porosity, somewhat higher than that of Ru/SAS.

Surface Chemistry
Both original and Ru-containing carbon materials were characterized by TPD. Table 2 includes the quantification of the obtained TPD profiles. The TPD-CO 2 and TPD-CO curves of the three Ru-containing catalysts are shown in Figure 1, while those corresponding to the carbon materials can be seen in Figure S4.
The evolution profiles of CO 2 and CO, produced by the decomposition of oxygen functional groups (OFG), can give information about the nature of such OFG. Those that decompose at lower temperature, mainly as CO 2 , have acidic character (carboxylic, anhydrides and lactones), and those that decompose as CO are weakly acidic, like phenol type groups, or basic, like carbonyl and quinone type groups. Based on the reported temperature intervals for the thermal decomposition of each type of group [29][30][31][32], Figure 1 shows their approximate distribution in the TPD profiles (see the figure caption for the identification of oxygen functional groups).
Energies 2020, 13, x FOR PEER REVIEW 4 of 15 the porosity by the abundant surface oxygen groups. This was previously reported for other carbon materials submitted to a similar treatment [28]. The incorporation of Ru produces only a slight decrease of the surface area and porosity of SA, SASu and SAS carbons, indicating that the supported Ru nanoparticles produce almost no blockage of the carbon porosity. Thus, comparing the Ru-containing catalysts, it can be observed that Ru/SA and Ru/SASu show a similar porosity, somewhat higher than that of Ru/SAS.

Surface Chemistry
Both original and Ru-containing carbon materials were characterized by TPD. Table 2 includes the quantification of the obtained TPD profiles. The TPD-CO2 and TPD-CO curves of the three Rucontaining catalysts are shown in Figure 1, while those corresponding to the carbon materials can be seen in Figure S4.
The evolution profiles of CO2 and CO, produced by the decomposition of oxygen functional groups (OFG), can give information about the nature of such OFG. Those that decompose at lower temperature, mainly as CO2, have acidic character (carboxylic, anhydrides and lactones), and those that decompose as CO are weakly acidic, like phenol type groups, or basic, like carbonyl and quinone type groups. Based on the reported temperature intervals for the thermal decomposition of each type of group [29][30][31][32], Figure 1 shows their approximate distribution in the TPD profiles (see the figure caption for the identification of oxygen functional groups).   [a] determined from data of (mmol O)/g calculated as (mmol CO)/g + (2 mmol CO 2 )/g. Moreover, [b] determined from deconvolution data, as the sum of quantification corresponding to SC, WC, CA, PH and LN functional groups.
Pristine SA carbon has an intermediate amount of OFG compared to other activated carbon materials that have been reported in the literature, but the large content of groups that evolve as CO can be pointed out. It can be observed that treatment A renders a moderated amount of surface oxygen functional groups (sample SASu), while treatment B resulted, as expected, in an important development of surface functional groups (sample SAS). Thus, the sum of evolved CO 2 and CO is, in SAS, more than double than in SASu.
A comparison of the TPD results corresponding to the carbon materials and the carbon supported Ru catalysts (Table 2 and Figure S4) shows that the amount of CO 2 + CO released from Ru/SA and Ru/SASu is larger than the amount released from the respective supports. This means that the preparation process of the supported Ru nanoparticles (impregnation and reduction treatment) leads to a certain restructuration of the carbon surface chemistry. This likely involves the creation of new groups and the transformation of previously existing ones [33,34]. Besides, the Ru nanoparticles can catalyze the decomposition and/or transformation of OFG during the TPD experiment and, thus, the TPD profiles are much more difficult to interpret because of that. In contrast, the evolution of CO 2 and CO decreases after Ru incorporation in the SAS carbon. In this case, the net balance of the effect of the catalyst preparation steps (impregnation and reduction) is the loss of a certain amount of OFGs from the surface of the highly oxidized SAS support.
Deconvolution of the TPD curves, according to the criteria from previous works [32,35,36], was performed in order to better identify the nature of functional groups, and to try to quantify all of them (the deconvoluted curves can be seen in Figure S5). As indicated above, the main OFG are carboxylic acid (weakly (WC) and strongly (SC) acidic), carboxylic anhydride (CA), phenol (PH) and lactone (LN), with acidic character, and carbonyl and quinone (CQ), with basic character.
The calculated amount of each type of functional groups in the carbon materials and in the Ru catalysts is shown as bar diagrams in Figure 2. The experimental error in these data is below 5%, estimated from the precision of data usually obtained in TPD experiments performed with the device used in this work.
It can be observed that, upon treatment A (sample SASu), basic CQ groups are mainly developed, followed by carboxylic anhydride (CA) groups, whereas treatment B (sample SAS) leads to an extensive development of carboxylic anhydride (CA) and phenol type (PH) groups. Regarding the effect of Ru incorporation, and compared to the respective supports, data of Figure 2 show that: in the case of the Ru/SA sample, the increase in the amount of CQ groups is very important and the increase in the amount of CA and PH groups is also relevant; in Ru/SASu, the content of all types of OFG, excepting SC and WC increases, and in Ru/SAS, in spite of the general decrease of the OFG amount, the CQ and LN groups content increases. It can be observed that, upon treatment A (sample SASu), basic CQ groups are mainly developed, followed by carboxylic anhydride (CA) groups, whereas treatment B (sample SAS) leads to an extensive development of carboxylic anhydride (CA) and phenol type (PH) groups. Regarding the effect of Ru incorporation, and compared to the respective supports, data of Figure 2 show that: in the case of the Ru/SA sample, the increase in the amount of CQ groups is very important and the increase in the amount of CA and PH groups is also relevant; in Ru/SASu, the content of all types of OFG, excepting SC and WC increases, and in Ru/SAS, in spite of the general decrease of the OFG amount, the CQ and LN groups content increases.
According to these data, the amount of acidic sites present in the studied samples has been estimated as sum of the amount of SC, WC, CA, PH and LN groups, and has been included in Table  2. This table shows that for both, the carbon supports and the analogous metal containing catalysts, the acidic groups content follows the relative order: SA < SASu < SAS.

XPS and TEM Analysis
XPS analysis of the three Ru containing catalysts was carried out to determine the electronic state of the supported Ru species, to identify potential sulfur species present, and to analyze the oxygen species, to complete the information obtained by TPD. Figure 3 shows the Ru 3p XPS spectra of the three studied catalysts.  According to these data, the amount of acidic sites present in the studied samples has been estimated as sum of the amount of SC, WC, CA, PH and LN groups, and has been included in Table 2. This table shows that for both, the carbon supports and the analogous metal containing catalysts, the acidic groups content follows the relative order: SA < SASu < SAS.

XPS and TEM Analysis
XPS analysis of the three Ru containing catalysts was carried out to determine the electronic state of the supported Ru species, to identify potential sulfur species present, and to analyze the oxygen species, to complete the information obtained by TPD. Figure 3 shows the Ru 3p XPS spectra of the three studied catalysts. It can be observed that, upon treatment A (sample SASu), basic CQ groups are mainly developed, followed by carboxylic anhydride (CA) groups, whereas treatment B (sample SAS) leads to an extensive development of carboxylic anhydride (CA) and phenol type (PH) groups. Regarding the effect of Ru incorporation, and compared to the respective supports, data of Figure 2 show that: in the case of the Ru/SA sample, the increase in the amount of CQ groups is very important and the increase in the amount of CA and PH groups is also relevant; in Ru/SASu, the content of all types of OFG, excepting SC and WC increases, and in Ru/SAS, in spite of the general decrease of the OFG amount, the CQ and LN groups content increases.
According to these data, the amount of acidic sites present in the studied samples has been estimated as sum of the amount of SC, WC, CA, PH and LN groups, and has been included in Table  2. This table shows that for both, the carbon supports and the analogous metal containing catalysts, the acidic groups content follows the relative order: SA < SASu < SAS.

XPS and TEM Analysis
XPS analysis of the three Ru containing catalysts was carried out to determine the electronic state of the supported Ru species, to identify potential sulfur species present, and to analyze the oxygen species, to complete the information obtained by TPD. Figure 3 shows the Ru 3p XPS spectra of the three studied catalysts.   [38,39]. This means that the reduced catalysts become partially oxidized upon exposition to air, being the proportion of metallic Ru respect to total Ru close to 50% (56%, 41% and 67% in Ru/SA, Ru/SASu and Ru/SAS catalysts, respectively; the differences cannot be considered as fully significant, because the time of air exposure after the reduction treatment was not exactly the same for the three catalysts). In any case, the oxidized species will probably be reduced again to the metallic state during the reaction, because of the presence of high-pressure hydrogen at 190 • C. The total amounts of Ru determined by XPS are 1.13wt%, 1.02wt% and 1.52 wt% in Ru/SA, Ru/SASu and Ru/SAS, respectively, which is in line with the nominal metal loading of the catalysts.  Figure 4 shows the O 1s XPS spectra deconvoluted according to the B.E. assignments reported in the literature [30,40,41]. It can be seen that they show mainly four signals corresponding to the C=O bond in quinone groups (about 531.0 ± 0.4 eV), C=O or -OH related to lactone or hydroxyl groups (532.0 ± 0.2 eV), C-OH attributed to phenol groups (at 533.0 ± 0.3 eV) and -COOH (534.0 ± 0.5 eV), assigned to carboxylic groups.
differences cannot be considered as fully significant, because the time of air exposure after the reduction treatment was not exactly the same for the three catalysts). In any case, the oxidized species will probably be reduced again to the metallic state during the reaction, because of the presence of high-pressure hydrogen at 190 °C. The total amounts of Ru determined by XPS are 1.13, 1.02 and 1.52 wt% in Ru/SA, Ru/SASu and Ru/SAS, respectively, which is in line with the nominal metal loading of the catalysts. Figure 4 shows the O 1s XPS spectra deconvoluted according to the B.E. assignments reported in the literature [30,40,41]. It can be seen that they show mainly four signals corresponding to the C=O bond in quinone groups (about 531.0 ± 0.4 eV), C=O or -OH related to lactone or hydroxyl groups (532.0 ± 0.2 eV), C-OH attributed to phenol groups (at 533.0 ± 0.3 eV) and -COOH (534.0 ± 0.5 eV), assigned to carboxylic groups. The quantification of the O1s spectra, as oxygen wt %, in all the mentioned oxygen-containing surface species, is presented in Table 3. The quantification of the O1s spectra, as oxygen wt %, in all the mentioned oxygen-containing surface species, is presented in Table 3. Considering that the main acidic groups determined by XPS are carboxylic and phenol type ones, the O wt% related to these groups (columns 3 and 4 in Table 3) has been summed up and assimilated to the amount of O in acidic oxygen functional groups. Data show that it increases from Ru/SA to Ru/SAS, in agreement with the TPD data. For a more proper comparison of XPS and TPD data, the amount of oxygen in acidic groups has been calculated as mmol/g of acidic groups (taking into account that column 4 corresponds to functional groups with two oxygen atoms). The calculated values are included in Table 3. Compared with analogous data of Table 2, it can be concluded that the results of both techniques are in line (the differences can be related with inherent differences in the fundamentals of analysis of the two techniques).
The S 2p XPS spectra of the Ru/SASu and Ru/SAS catalysts reveal the presence of sulfonic groups (peak at~168.5 ± 0.1 eV) [42]. However, those spectra present a lot of noise, which makes the quantification of sulfur species quite imprecise. Figure 5a-f show TEM images obtained for the Ru catalysts. It must be mentioned that although, in general, well dispersed Ru nanoparticles have been observed (Figure 5a-c), some agglomeration of particles has also been found (Figure 5d-f), particularly in the case of the catalyst prepared with the most oxidized support. The particle size distribution has been plotted as bar diagrams in Figure 5g-i, being 1.3, 1.4 and 1.6 nm the average particle sizes in Ru/SA, Ru/SASu and Ru/SAS catalysts, respectively. Figure S6 shows a TEM image of the original SA activated carbon.
In general, TEM data indicate that the impregnation method used was successful for the formation of small Ru nanoparticles (average size lower than 2 nm) on SA, SASu and SAS carbon materials, and it seems that the particle size slightly increases with the support oxidation. This can be related to the anchorage of the Ru precursor species on the OFG, and the effect of their partial decomposition during the reduction heat treatment, which could lead to some metal sintering [43][44][45].  Table 4 shows the obtained cellulose conversion and yield of the main reaction products values. Other products (not shown here) have also been obtained in a very low amount, and most of them could not be identified. Figure 6 shows a simplified scheme of the reaction pathway from cellulose to the products presented in Table 4.   Table 4 shows the obtained cellulose conversion and yield of the main reaction products values. Other products (not shown here) have also been obtained in a very low amount, and most of them could not be identified. Figure 6 shows a simplified scheme of the reaction pathway from cellulose to the products presented in Table 4.  Table 4 shows the obtained cellulose conversion and yield of the main reaction products values. Other products (not shown here) have also been obtained in a very low amount, and most of them could not be identified. Figure 6 shows a simplified scheme of the reaction pathway from cellulose to the products presented in Table 4. In the blank test (without catalyst), cellulose conversion was 35%, and small amounts of glucose and hydroxymethylfurfural (HMF) were formed (Table 4). In the tests carried out with the carbon supported Ru catalysts, a noticeable increase of cellulose conversion compared with the blank  In the blank test (without catalyst), cellulose conversion was 35%, and small amounts of glucose and hydroxymethylfurfural (HMF) were formed (Table 4). In the tests carried out with the carbon supported Ru catalysts, a noticeable increase of cellulose conversion compared with the blank experiment and a high sorbitol yield have been achieved. The experimental error in these data is below 5%. It has been estimated from the deviation determined when catalytic activity tests have been repeated.

Catalytic Conversion of Cellulose
Selectivity data have been plotted in Figure 7.
Energies 2020, 13, x FOR PEER REVIEW 10 of 15 experiment and a high sorbitol yield have been achieved. The experimental error in these data is below 5%. It has been estimated from the deviation determined when catalytic activity tests have been repeated. Selectivity data have been plotted in Figure 7. Data from Table 4 show that, among the Ru containing catalysts, Ru/SA is the best performing one in terms of cellulose conversion and sorbitol yield. As shown in Figure 7, the three catalysts are quite selective to sorbitol (selectivity above 75%), being Ru/SA highly selective (91%). It can be pointed out that catalysts Ru/SASu and Ru/SAS produce a significant amount of by-products.
Catalyst Ru/SA was tested for recyclability. After the first run, the catalyst and the unreacted cellulose were recovered from the reaction media by filtration, and after washing and drying, they Data from Table 4 show that, among the Ru containing catalysts, Ru/SA is the best performing one in terms of cellulose conversion and sorbitol yield. As shown in Figure 7, the three catalysts are Energies 2020, 13, 4394 10 of 14 quite selective to sorbitol (selectivity above 75%), being Ru/SA highly selective (91%). It can be pointed out that catalysts Ru/SASu and Ru/SAS produce a significant amount of by-products.
Catalyst Ru/SA was tested for recyclability. After the first run, the catalyst and the unreacted cellulose were recovered from the reaction media by filtration, and after washing and drying, they were used in a second run in the same conditions (adding the necessary amount of cellulose). In the second run, cellulose conversion was 45% and selectivity to sorbitol was 73%. This means that the catalyst preserves a relatively high activity and selectivity, which makes it reusable.
In a previous work dealing with the catalytic activity of SA, SASu and SAS carbons for cellulose hydrolysis [46], it was concluded that cellulose conversion and selectivity to glucose depend on the amount and type of surface OFG. In that study, the SAS carbon was found to lead to the highest cellulose conversion and glucose yield (61% and 52%, respectively). However, in the case of the Ru catalysts, the surface chemistry seems to have the opposite effect. As mentioned above, the presence of a high amount of OFG might lead to a less effective dispersion of the Ru particles, which could be associated with a lower activity. Moreover, some OFG, mainly those of acidic character (more abundant in Ru/SASu and Ru/SAS samples) may direct, likely assisted by the metal sites, the reaction to by-products through the conversion of glucose or sorbitol into other products. Furthermore, the presence of sulfonic groups, although in a low proportion, can contribute to acid catalyzed transformations that led to by-products.
Thus, in summary, the Ru/SA catalyst was found to be effective in enhancing the production of C6 polyols (sorbitol and mannitol), hindering the generation of by-products. It shows a good catalytic capability for the one pot hydrolytic hydrogenation of cellulose, similar or even higher than that shown by other catalysts reported in the literature, as some of those presented in the review work of Shrotri et al. [47] For comparison purposes, some examples of literature reported results obtained with carbon supported Ru catalysts (including reaction conditions) are summarized in Table 5 and commented on next. and the reaction time was long (24 h). In the work of Zhang et al. [16] (entry 5), Ru was supported on a mesoporous carbon, but the obtained results were relatively poor; a significant improvement (95% cellulose conversion and 52% sorbitol yield) was achieved combining the Ru/MC catalysts with a zirconium phosphate (1.8 g per gram of cellulose). The recent work of Li et al. [13] that deals with Ru catalysts supported on carbonized cassava dregs (CCD), reported the need of the carbon sulfonation to achieve a high cellulose conversion and sorbitol yield (entries 6 and 7). Conditions of the process include, among others, harsh sulfuric acid treatment of the CCD material and high catalyst dosage. Finally, Rey-Raap et al. [15] (entry 8) reported very good sorbitol yield, using catalysts supported on a hybrid carbon material prepared with a glucose derived activated carbon and carbon nanotubes. The authors used ball-milled cellulose (milling conditions not indicated), 5 h reaction time, and a reaction temperature higher than in other reported works.
Regarding preparation conditions, with the exception of catalysts prepared by Kobayashi et al. [19] (entry 1) that use Ru(NO)(NO 3 ) 3 ; in all cases, RuCl 3 has been used as Ru precursor. Additionally, like in the present work, impregnation has been the general preparation procedure employed. It can be mentioned that, in the case of catalysts presented in entries 2 to 7, the catalysts reduction treatment temperature is higher than in the present work (300, 350 or 400 • C, vs. 250 • C), Rey-Raap et al. [15] also reduced the catalysts at 250 • C, and in the work of Kobayashi et al. [19], the catalysts were reduced at 180 • C, but suspended in water and under 4 MPa H 2 , being then collected by filtration and dried, which adds some steps to the preparation process.
Some of the studies reported in Table 5 include reusability tests. After reaction, Li et al. [13] separated the mixture of catalyst and unreacted cellulose from the solution, and after washing and drying, fresh cellulose was added to start a second run. They repeated the process for up to five recycles, and found about a 10% decrease in sorbitol yield [13]. A slight sorbitol yield decrease (3%) was also observed by Komanoya et al. [21] in three reusing experiment tests, and Deng et al. [22] also found a decrease of the sorbitol yield (10%), especially after the first reuse. This behaviour is similar to the one found for the Ru/SA catalysts of the present work, in which sorbitol yield in the second run was about 15% lower than in the first one. However, Rey-Raap et al. [15] report very good reusability of their Ru catalyst supported on a hybrid carbon material (sorbitol yield kept above 60%).
To summarize, some interesting conclusions can be extracted, comparing our results with those dealing with carbon-based catalysts previously published. For example, the sorbitol yield obtained in the present work is higher, and it has been obtained in a much shorter time than in the study reported by Kobayashi et al. [19] (entry 1). The high cellulose conversion and the much lower sorbitol yield obtained by these authors indicate that their process is not very selective. The good results obtained by Komanoya et al. [21] (entry 3) require, as already mentioned, four days' mix-milling and, comparing the results of the present work with those of Deng et al. [22] (entry 4), a higher sorbitol yield has been obtained avoiding acid cellulose pretreatment, in a shorter reaction time and with a higher S/Ru ratio. The good results of Rey-Raap et al. [15] (entry 8) are undoubtedly very interesting, but compared with the present work, they have a required higher temperature and reaction time.
This comparison allows concluding that the catalysts prepared in this work are competitive in relation to other catalysts developed for the hydrolytic hydrogenation of cellulose, pointing out that both the catalysts' preparation and the reaction conditions used are mild, and can be considered as environmentally friendly.

Conclusions
The combined hydrolytic hydrogenation of cellulose into sorbitol was successfully achieved using catalysts prepared by supporting Ru nanoparticles (1 wt% Ru) on mesoporous carbon materials (the commercial activated carbon SA, and SASu and SAS carbons, both obtained by SA functionalization). The sulfonation treatment creates a moderated amount of oxygen functional groups on the carbon surface, and only a slight modification of the porous structure of the SA carbon. On the other hand, the oxidation treatment leads to an important development of surface functional groups, and a significant decrease in the adsorption capacity. Besides, the amount and type of the surface functional groups of the carbon materials becomes modified as a result of the Ru incorporation process. In spite of these modifications, it has been concluded that the mesopore volume remains high in the three catalysts. The Ru nanoparticles are small and well dispersed, and about 50% Ru is present in zero valent state even after air exposition of the catalysts.
The three catalysts exhibited high cellulose conversion and very good selectivity to sorbitol under relatively mild reaction conditions. The Ru/SA catalyst is the best performing one, with 52% cellulose conversion and a very high sorbitol selectivity (91%). The differences in surface chemistry seem to determine the observed differences in the catalytic behavior, and although a positive effect of a large amount of acidic OFG was foreseen, the results allow one to conclude that in the Ru containing catalysts, such groups can catalyze the formation of by-products, and are responsible for lower selectivity.
The findings of this study highlight the performance of the Ru/SA catalyst in the one pot hydrolytic hydrogenation of cellulose, which surpasses those of many other bifunctional catalysts. Moreover, it can be pointed out that both the catalyst preparation and the reaction conditions used in this work can be regarded as economically convenient and environmentally friendly.
Supplementary Materials: The following are available online at http://www.mdpi.com/1996-1073/13/17/4394/s1, Figure S1. Reactor scheme, Figure S2. Example of HPLC data obtained in the catalytic activity experiment carried out with the Ru/SA catalyst, Figure S3. N 2 adsorption isotherms at −196 • C of the carbon supports and Ru catalysts, Figure S4. TPD profiles for supported Ru on carbon catalysts and carbons support, Figure S5. Deconvolution of the TPD profiles obtained for the supported Ru catalysts (SC: Strong acidic carboxyl groups, WC: Weak acidic carboxyl groups, CA: Carboxylic anhydride groups, CQ: Carbonyls or Quinones, PH: Phenol, LN: Lactones). Figure S6. TEM image of the SA activated carbon.