Naringin Supplementation during Pregnancy Induces Sex and Region-Specific Alterations in the Offspring’s Brain Redox Status

Research has shown the beneficial effects of naringin supplementation to adult rodents, which can ameliorate oxidative stress in disease models. However, evidence has demonstrated that polyphenol supplementation induced detrimental effects when consumed during sensitive periods of development, such as pregnancy. Therefore, we investigated the effect of maternal naringin supplementation during pregnancy on the offspring’s cerebral redox status. Pregnant Wistar rats were divided into control and naringin groups and supplemented from gestational day 15 to gestational day 21. On postnatal days 1, 7, and 21, offspring were euthanized, and the prefrontal cortex, hippocampus, striatum, and cerebellum dissected. On postnatal day 1, maternal naringin supplementation positively modulated the pups’ brain redox status. On postnatal day 7, a pro-oxidative milieu was observed in the offspring’s striatum and cerebellum in a sex-dependent manner, even though the prefrontal cortex and hippocampus were not negatively affected. Besides, the alterations observed on postnatal day 7 did not persist up to weaning. Our findings demonstrated that the effect induced by naringin supplementation in the brain redox status differed according to the period of development in which naringin was consumed since the beneficial effects usually found in the adult rodents became detrimental when the supplementation was applied during pregnancy.


Introduction
Naringin, which is a glycosylated flavonoid, is commonly found in Chinese herbal medicines, citrus fruits, and derivative beverages [1]. During the past years, it has been demonstrated that naringin can promote neuroprotection in experimental models of cognitive dysfunction, such as Parkinson's disease, epilepsy, and Alzheimer's disease mainly by reducing oxidative stress and improving neuronal survival [2][3][4][5]. Additionally, though data from clinical and observational research is still limited, they also show that citrus fruit extract, grapefruit, and even naringin supplements can reduce total cholesterol, lowdensity lipoprotein (LDL), and improve blood pressure [6][7][8][9]. Therefore, this whole body of evidence suggests that flavonoid intake, including naringin, can induce beneficial effects and promote health in the general population when consumed in the diet or by flavonoid-based supplements.

Experimental Protocol
The female Wistar rats were mated in a proportion of 1 male to 2 females per cage, and the pregnancy diagnosis was confirmed by the presence of sperm in the vaginal smear and it was considered as the gestational day zero [32]. Pregnant rats were randomly divided into two experimental groups treated by oral gavage: control (equivalent volume of distilled water) and naringin groups (100 mg/kg/day). This dose was selected based on studies that have demonstrated modulation of the redox status by naringin in different brain regions in adult rodents [33,34].
The naringin supplementation occurred during the third week of pregnancy, between gestational days 15 and 21. Such period was chosen based on reports demonstrating that the intrauterine development of the brain regions analyzed here predominantly occurs during the third week of gestation; therefore, the maternal naringin supplementation was administered during such a critical window of development [35][36][37]. The delivery date was considered as postnatal day 0. After delivery, the pups were randomly selected and culled to maintain litters of eight pups per dam with the same proportion of males and females. From delivery up to weaning each dam was housed with its own litter.
On postnatal days 1, 7, and 21 the male and female pups were randomly picked from each control and naringin litter and euthanized by decapitation without anesthesia to avoid tissue chemical contamination. The prefrontal cortex, hippocampus, striatum, and cerebellum were dissected and stored at −80 • C. Here we considered the litter as the experimental unit; therefore, all the analyses were undertaken with samples from pups obtained from different litters to avoid the litter effect [38]. For each biochemical analysis, we have used between 6 and 8 pups as the experimental "n".

Sample Processing
The brain regions were homogenized (1:10 w/v) in 20 mM sodium phosphate buffer (Sigma Chemical Co., St. Louis, MO, USA), pH 7.4, containing 140 mM KCl (Sigma Chemical Co., St. Louis, MO, USA), and centrifuged at 750× g for 10 min at 4 • C. The pellet was discarded, and the supernatant was used for the biochemical analyses. DCFH converted from H 2 DCF-DA, was oxidized further by cellular oxidants, mainly H 2 O 2 , producing DCF, which is a fluorescent compound. DCF fluorescence was detected at 488 nm excitation and 525 nm emission. A DCF standard curve from 0.25 to 10 mM was also used in parallel. Data are expressed as nmol/mg protein [39].

Antioxidant Enzymes Assays
The antioxidant enzyme activities were evaluated as described below. Moreover, the SOD/GPx ratio was mathematically calculated by dividing the SOD activity by the GPx activity of the same sample in the following formula: SOD/GPx ratio = (SOD activity/GPx activity) × 100 [40] 2.6.6. Glyoxalase 1 Activity Glyoxalase 1 (GLO1, EC 4.4.1.5) activity was measured by following the increase in the S-D-lactoylglutathione at 240 nm. The reaction medium consisted of 60 mM sodium phosphate buffer, pH 6.6, with reduced glutathione 0.01 M, and methylglyoxal 0.01 M (Sigma Chemical Co., St. Louis, MO, USA). A GLO1 unit is defined as the amount of enzyme needed to catalyze the formation of 1 µmol of S-D-lactoylglutathione per minute, and the specific activity is represented as units/mg protein [44].

Total Reduced Glutathione Content
First, high molecular weight proteins in the tissue homogenate were precipitated with meta-phosphoric acid (1:1, v/v, Sigma Chemical Co., St. Louis, MO, USA), and centrifuged at 5000× g for 10 min at 25 • C. Reduced glutathione (GSH) present in the supernatant reacted with the fluorophore o-phtaldialdehyde (7.5 mM) prepared in 100 mM sodium phosphate buffer, pH 8.0, with 5 mM EDTA. The fluorescence was read at excitation and emission wavelengths of 350 nm and 420 nm, respectively, using the SpectraMax Gemini XS Fluorescence microplate reader (Molecular Devices, Sunnyvale, CA, USA). A standard GSH curve ranging from 0.001 to 1 mM was prepared and a blank sample was performed in parallel. Data were expressed as nmol of GSH/mg protein [45].

Protein Determination
The total protein content of the samples was measured according to LOWRY et al. (1951) modified by PETERSON et al. (1977) and adapted to 96 well plates using bovine serum albumin as the standard [46,47]. Briefly, the samples were diluted in ultra-pure water in a proportion of 1/20 and then incubated with Lowry solution for 10 min under constant agitation of 30 g and temperature of 25 • C. Following the 10 min incubation, the 0.08 M folin solution (Dinâmica, Indaiatuba, São Paulo, Brazil). was added and the samples incubated for 30 min under constant agitation of 30 g and temperature of 25 • C in a dark room. The samples were read at 750 nm wavelength and the protein content was expressed as mg/mL.

Statistical Analysis
The analysis was performed by the IBM SPSS 22.0 program (SPSS Inc., Chicago, IL, USA). The data were tested for normality and the outliers exceeding ±2 standard deviations were excluded when necessary. All data were analyzed by the two-way analysis of variance (ANOVA) with maternal naringin supplementation (*) and the offspring's sex (#) as the two main independent factors. The ANOVA tests were followed by Sidak's multiple comparison test when interactions were statistically significant. All data are expressed as mean ± standard error of the mean (S.E.M.) and considered statistically significant when p < 0.05. All the descriptive statistical results are available within the Supplementary Materials in Tables S1-S4.

Naringin Supplementation during the Third Week of Pregnancy Positively Modulate the Males' Prefrontal Cortex Redox Status on Postnatal Day 7
On postnatal day 1, maternal naringin supplementation during pregnancy induced alterations in the offspring's prefrontal cortex redox status. As demonstrated in Figure 1d, both male and female pups born to naringin-supplemented dams showed increased GPx activity (supplementation effect, p = 0.024), even though no other supplementation-associated alterations were observed. Interestingly, our results also demonstrated sex-related differences suggesting that males had increased oxidative status than females since they showed higher total oxidants level (sex effect, p < 0.001), GPx (sex effect, p = 0.003), and GLO1 (sex effect, p = 0.048) activities (Figure 1a,d,f, respectively). Meanwhile, females had a higher SOD/GPx ratio (sex effect, p = 0.029) in the prefrontal cortex on postnatal day 1 ( Figure 5a). Moreover, when analyzing the offspring's prefrontal cortex redox status on postnatal day 7, there were observed sex-specific alterations induced by maternal naringin supplementation. At this age, both male and female offspring born to supplemented dams had increased GSH content (supplementation effect, p = 0.012) in the prefrontal cortex ( Figure 1b); however, an interaction between sex and supplementation was observed in the SOD (interaction, p = 0.002) and GPx (interaction, p = 0.008) activities, demonstrating that male pups born to naringin supplemented rats had increased activities compared to males born to control dams (Figure 1c,d, respectively). Moreover, the naringin males showed higher SOD (interaction, p = 0.002) and GPx (interaction, p = 0.008) activities when compared to the naringin females. The above supplementation-related alterations were accompanied by sex differences in the prefrontal cortex's redox status in which females had higher SOD (interaction, p = 0.002) and GLO1 (interaction, p = 0.026) activities (Figure 1c  Interestingly, on postnatal day 21, the total number of supplementation-associated alterations reduced, and no interaction between sex and supplementation was observed. With exception of the SOD/GPx ratio (supplementation effect, p = 0.022) (Figure 5a), which was increased in the prefrontal cortex of pups born to naringin-supplemented dams, no other supplementation-related alteration was observed in the redox parameters evaluated in our study ( Figure 1). In addition, sex-related differences were found in the SOD (sex effect, p = 0.011) and GPx (sex effect, p = 0.009) activities, which were higher in female than in male pups in the prefrontal cortex on postnatal day 21 (Figure 1c,d, respectively).

Maternal Naringin Supplementation during Pregnancy Triggers Sex-Independent Redox Alterations in the Offspring's Hippocampus
In the hippocampus, maternal naringin supplementation during pregnancy induced alterations in both male and female pups on postnatal day 1. Here, the offspring born to supplemented dams showed reduced GSH content (supplementation effect, p = 0.001), which was accompanied by increased GPx activity (supplementation effect, p = 0.003), in the hippocampus (Figure 2b,d, respectively), though no other supplementation-associated alteration was observed in the redox status at this postnatal age ( Figure 2). In addition, sex-related differences in which males showed higher oxidants (sex effect, p = 0.006), GSH content (sex effect, p = 0.002), SOD (sex effect, p = 0.014), and GPx (sex effect, p < 0.001) activities compared to females were also observed in the offspring's hippocampus on postnatal day 1 (Figure 2a-d, respectively).
Similarly, the alterations elicited by maternal naringin supplementation in the hippocampus' redox status on postnatal day 1 also persisted on postnatal day 7, as shown in Figure 2. The GSH content (supplementation effect, p = 0.002) remained reduced while the GPx activity (supplementation effect, p = 0.026) was still increased on postnatal day 7 (Figure 2b,d, respectively), in addition to other supplementation-related effects that were observed in the GLO1 activity (supplementation effect, p < 0.001), which increased ( m), and in the SOD/GPx ratio (supplementation effect, p = 0.013), which reduced in the offspring born to supplemented rats (Figure 5b). The above alterations were accompanied by sex-related differences in the total oxidants (sex effect, p < 0.001) and GPx activity (sex effect, p < 0.001), that were higher in females (Figure 2a,d, respectively), and in the SOD/Gpx ratio (sex effect, p = 0.005), which was higher in male pups (Figure 5b).
Although on postnatal day 7 the pups' hippocampal redox status was highly affected by maternal naringin supplementation, most of these alterations did not persist up to postnatal day 21. While the previous alterations found in the GSH content, GPx activity (Figure 2b,d, respectively) and in the SOD/GPx ratio (Figure 5b) did not show any significant difference on postnatal day 21, the GLO1 activity (supplementation effect, p = 0.039) reduced in the offspring born to naringin-supplemented dams, which contrasts from the increased GLO1 activity previously detected on postnatal day 7 ( Figure 2f). Besides, sex-related differences were observed on postnatal day 21 in which males had higher oxidants level (sex effect, p = 0.045), GSH content (sex effect, p = 0.006), and CAT activity (sex effect, p = 0.037) in the hippocampus when compared to the female pups (Figure 2a

Naringin Supplementation during Pregnancy Induces a Pro-Oxidative Shift in the Male
Offspring's Striatum on Postnatal Day 7 On postnatal day 1, the offspring born to naringin supplemented rats showed reduced total oxidants content (supplementation effect, p < 0.001) in the striatum (Figure 3a), even though no other supplementation-related alterations in the redox status were found at this postnatal age (Figure 3). Such alteration was also accompanied by a sex-associated difference in which females demonstrated higher oxidants content compared to males (sex effect, p = 0.007) also on postnatal day 1 (Figure 3a).  Moreover, on postnatal day 7, our results showed that maternal naringin supplementation during pregnancy affected the pups' redox status in a sex-specific manner in the striatum. An interaction between supplementation and sex was observed in the total oxidants content (interaction, p = 0.008) in which naringin male pups showed increased oxidants level compared to control males, even though the same effects were not observed in the female pups' striatum ( Figure 3a). Additionally, the naringin male pups had higher oxidants content when compared to the naringin female pups (interaction, p = 0.008). Similarly, the GLO1 and CAT activities also showed interactions between supplementation and sex (interaction, p = 0.020; p = 0.049; respectively), in which male pups born to naringin-supplemented rats had increased GLO1 activity compared to control males and naringin females (Figure 3f), and females born to supplemented dams showed decreased CAT activity compared to control female pups (Figure 3e).
In addition to the interactions between supplementation and sex detected on postnatal day 7, we also observed other supplementation and sex-related effects in the offspring's striatum at this postnatal age. As shown in Figure 3d and 5, the maternal naringin supplementation during pregnancy also induced an increase in the GPx activity (supplementation effect, p = 0.003), which probably led to a reduction in the SOD/GPx ratio (supplementation effect, p = 0.001) in both male and female pups (Figure 5c). Moreover, a sex-associated difference was observed in which males showed higher SOD activity (sex effect, p = 0.002) compared to the female pups (Figure 3c).
Despite naringin supplementation induced several alterations in the redox status in the offspring's striatum on postnatal day 7, this same pattern was not observed on postnatal day 21, since the only effect elicited by the maternal supplementation was the reduction in the GSH content (supplementation effect, p = 0.042), as shown in Figure 3b. Besides, no sex-related differences were observed between male and female pups on postnatal day 21.

Maternal Naringin Supplementation during the Third Week of Pregnancy Induces a Pro-Oxidative Milieu in the Female Pups' Cerebellum on Postnatal Day 7
As demonstrated by our findings, the maternal naringin supplementation during pregnancy did not induce alterations in the offspring's cerebellum redox status on postnatal day 1, as shown in Figure 4. However, sex-related differences were observed at this postnatal age, in which males showed higher GSH levels (sex effect, p = 0.003), GPx (sex effect, p = 0.004), and CAT (sex effect, p < 0.001) activities (Figure 4b,d,e, respectively), while females displayed higher SOD (sex effect, p < 0.001) activity ( Figure 4c) and SOD/GPx ratio (sex effect, p < 0.001) in the cerebellum (Figure 5c).
On postnatal day 7, in contrast to the findings observed on postnatal day 1, the offspring were affected by maternal naringin supplementation, though the effect occurred in a sex-specific manner. Both female and male pups born to naringin supplemented rats demonstrated increased SOD (supplementation effect, p = 0.001), GPx (supplementation effect, p = 0.012), and GLO1 (supplementation effect, p = 0.015) activities (Figure 4c,d,f, respectively) in the cerebellum one week after birth, and such enzymatic alterations were accompanied by a significant interaction between supplementation and sex in the total oxidants level (interaction, p = 0.021). This effect showed that naringin females had increased oxidants compared to the control female pups, but naringin males did not show any alteration regarding the same variable (Figure 4a). Moreover, the naringin females displayed higher oxidants when compared to the naringin males (interaction, p = 0.021).
Moreover, such interaction also demonstrated that the control females had higher oxidants content in the cerebellum when compared to the control males on postnatal day 7 (Figure 4a). This difference was followed by other sex-related effects in which females showed higher GSH content (sex effect, p = 0.002), SOD activity (sex effect, p < 0.001) (Figure 4b,c, respectively), and SOD/GPx ratio (sex effect, p < 0.001) (Figure 5c), while males showed a higher GPx activity (sex effect, p < 0.001), demonstrating that on postnatal day 7 the female offspring possessed a higher oxidative status than males of the same age (Figure 4d).   Figure 4a). This difference was followed by other sex-related effects in which females showed higher GSH content (sex effect, p = 0.002), SOD activity (sex effect, p < 0.001) (Figure 4b,c, respectively), and SOD/GPx ratio (sex effect, p < 0.001) (Figure 5c), while males showed a higher GPx activity (sex effect, p < 0.001), demonstrating that on postnatal day 7 the female offspring possessed a higher oxidative status than males of the same age (Figure 4d).  Additionally, the redox alterations induced by maternal naringin supplementation in the female's cerebellum on postnatal day 7 did not persist up to postnatal day 21, since we did not observe any supplementation-associated effect at this age, as demonstrated in Figure 4. In contrast, the male pups showed two significant interactions between supplementation and sex in the SOD (interaction, p = 0.035) and GLO1 (interaction, p = 0.031) activities on postnatal day 21, in which male pups born to naringin-supplemented rats showed decreased activities of both enzymes when compared to the control males of the same postnatal age (Figure 4c,f, respectively).

(
Lastly, the sex-related differences found in the offspring's cerebellum on postnatal day 21 showed that the male pups, differently from postnatal day 7, have increased redox status than the female pups from the same postnatal age (Figure 4). Here, males showed higher total oxidants level (sex effect, p = 0.022), GSH content (sex effect, p = 0.004), GPx (sex effect, p = 0.006), and GLO1 (interaction, p = 0.031) activities (Figure 4a,b,d,f, respectively), while the female pups had higher SOD/GPx ratio (sex effect, p = 0.006) at this postnatal age (Figure 5c).

Discussion
Although polyphenols can induce beneficial effects when supplemented to adult rodents, recent evidence has been demonstrating that polyphenol supplementation can induce detrimental effects if consumed during sensitive periods of development, such as pregnancy, which can affect the fetal intrauterine development and lead to physiological alterations during the postnatal life [21,[48][49][50]. Therefore, since naringin is a polyphenol able to modulate the redox status, we hypothesized that maternal naringin supplementation during the third week of pregnancy can induce alterations in the offspring's brain redox status during postnatal life.
Considering the total number of alterations found in our model of maternal naringin supplementation, we notice that they are not proportionally distributed among the brain regions and the postnatal ages evaluated here. Such observation demonstrates that the effects induced by maternal naringin supplementation during the third week of pregnancy are still active during offspring's postnatal development, mainly on postnatal day 7. However, most of the redox alterations observed in the younger pups did not persist up to postnatal day 21, which suggests that they are transitory and did not persist along with the offspring's brain development. Similarly, AUGUST et al. (2018) also observed the same pattern in which maternal supplementation with naringenin, the aglycone form of naringin, did not induce alterations in the offspring's brain redox status on postnatal day 21, even though only male pups were evaluated [51].
Moreover, on postnatal day 1, the maternal naringin supplementation during the third week of pregnancy induced a positive modulation in the offspring's brain redox status that occurred in a region-specific manner. As shown in Table 1, the reduced total oxidants content in the pups' striatum was not accompanied by alterations in the antioxidant enzymes activity and GSH level, even though the hippocampus and the prefrontal cortex showed the opposite pattern: alterations in the GPx and GSH, and no changes in the oxidants level with 1 week of maternal naringin supplementation. The total oxidants content is commonly assessed by the DCFH, and together with the antioxidant enzymes activity and GSH levels, they can demonstrate the redox status of a cell or tissue, thereby suggesting that in our model, with exception of the cerebellum, the maternal naringin supplementation triggered an antioxidant milieu in the pup's brain regions on postnatal day 1 [52]. Accordingly, evidence elsewhere has also demonstrated that naringin supplementation can positively modulate the redox status in adult rat's brains, improving antioxidants defenses and reducing the oxidants content, though it occurred with more than one week of naringin intake [34,53,54]. Table 1. Summary of the alterations induced by maternal naringin supplementation during the third week of gestation in the offspring's brain redox status during postnatal development. ↓: means the given parameter was significantly lower in the naringin pups compared to the control pups, ↑: means the given parameter was significantly higher in the naringin pups compared to the control pups.
Interestingly, even though naringin supplementation during pregnancy induced an antioxidant effect in the pup's brain on postnatal day 1, such alteration did not predominantly occur on postnatal day 7. In the cerebellum and striatum, naringin positively regulated most of the antioxidant enzymes in the male and female offspring; however, both brain regions also displayed a sex-dependent pro-oxidant effect demonstrated by the higher oxidants content. Such increase is in contrast with most published data demonstrating that naringin promotes neuroprotection by reducing oxidants and increasing antioxidant defenses when supplemented to adult rats submitted to different models of cognitive dysfunction [33,[55][56][57][58][59]. However, it is also important to consider that in our model naringin alone increased the total oxidants content, a pro-oxidative property of flavonoids that was already demonstrated in vitro in a dose-dependent manner [60,61]. Thereby, such prooxidative milieu may have induced the increase in the antioxidant enzyme activities as a compensatory mechanism in the females' cerebellum and the males' striatum, which suggests that regarding these brain regions, female and male offspring were negatively affected by naringin consumption during gestation [62].
Additionally, even though naringin induced a prooxidative effect in the pups' cerebellum and striatum, such alterations were not observed in the prefrontal cortex and hippocampus on postnatal 7 since these brain regions exhibited a positive modulation of the redox status. As shown in Table 1, maternal naringin supplementation reduced the GSH content in the offspring's prefrontal cortex in both sexes, but the male pups also displayed higher SOD and GPx activities, which has been demonstrated in adult male rats' cerebral cortex in a dose-dependent manner [54]. Similarly, the pups' hippocampal antioxidant enzymes were positively modulated while the GSH content was reduced on postnatal day 7. The GSH is the main non-enzymatic antioxidant in the cells that can directly react with reactive species or participate in enzymatic pathways, which suggests that such lower GSH levels might have occurred due to the positive regulation exerted by naringin in the GPx and GLO1 activities in the hippocampus from naringin-exposed pups [42,45,63].
Moreover, further analyses of the brain redox status on postnatal day 21 demonstrated that the naringin-induced alterations observed on postnatal day 7 were transient and did not persist up to weaning. Even though most of the brain regions displayed negative modulations of the GSH, GLO1, and SOD/GPx ratio in both sexes, the cerebellum showed sex-dependent effects in which only males had lower SOD and GLO1 activities. Such alterations observed on postnatal day 21 demonstrate that naringin negatively modulated such parameters, which contrasts with data showing that naringenin supplementation to pregnant rats during gestation did not induce redox alterations in the male pups' cerebellum and hypothalamus, though the naringenin dose used was 50 mg/kg/day [51].
Despite the alterations observed in our model, the underlying mechanisms by which naringin or flavonoid supplementation during pregnancy can modify fetal redox homeostasis remain largely unknown. It is well established that the redox signaling is essential during neurodevelopment since the transition from neural stem cells (NSC) to a neuron involves a metabolic shift from a glycolytic to a more oxidative based metabolism, which consequently increases the amount of mitochondrial-derived reactive oxygen species (ROS) [64,65]. Moreover, other sources of ROS, such as the NADPH-oxidase enzyme family, have also been proposed to play particularly important roles in inducing the neurogenic process [66][67][68]. Consequently, such an increase in the ROS induces the activation of several signaling cascades involved in the commitment of the NSC and its differentiation into a fully grown neuron [68][69][70][71].
Although the transient increase in ROS is essential to induce the commitment of the NSC, such a process needs to be tightly regulated to maintain a physiological and tolerable intracellular level of reactive species [72]. On one hand, persistent and particularly high amounts of ROS, which exceed a physiologically acceptable range, can induce oxidative stress, hinder axonal growth, and impair neurodevelopment, while on the other hand, low ROS amounts have been linked to the maintenance of the quiescent state of the NSC, which reduces its differentiation into neurons [67,73,74].
In addition, the neurogenesis in the developing rat brain can also be affected by alterations in the neuronal redox status. Both the deletion of the PR domain containing 16 (PRDM16) and the mitochondrial uncoupling protein 2 (UCP) in knockout mice during embryonic development strongly increased ROS levels, which were associated with abnormal cortical lawyer thickness and development [75,76]. Other cortical and hippocampal neurodevelopmental alterations related to increased ROS content have also been reported by a similar study during embryonic and adult life, which resulted in abnormal motor behavior along with memory and learning defects [77]. However, the abnormal decrease in the ROS levels during brain development has also been associated with histopathological abnormalities in the neonatal rat's cerebellum, which were accompanied by altered motor behavior [78]. Such findings demonstrate the important role that ROS plays as signaling molecules mediating the developing brain neurogenesis. More importantly, they also suggest that in healthy animals, neither a positive nor a negative modulation of the brain's redox status is beneficial to neurodevelopment, since both high and low amounts of reactive species have been shown to alter neurogenesis and induce anatomical and behavioral defects.
Since flavonoids are redox active molecules, they possess a scavenger activity and can directly react with ROS, as well as modulate cell signaling pathways that regulate the expression of several cellular components of the redox network [79][80][81]. It has been demonstrated that flavonoids and their metabolites have already been detected in the breast milk, amniotic fluid, and rat fetuses, suggesting they can cross the placental barrier and exert their redox regulation directly in the fetal tissues, and consequently modulating the fetal redox status, which can possibly interfere during normal neurodevelopment [82][83][84]. Moreover, maternal resveratrol supplementation has been shown to modify the methylation levels of the factor nuclear kappa β (NF-κβ) promoter region and the gene expression of the peroxisome proliferator-activated receptor gamma coactivator 1-alpha (Pgc-1α) and heme oxygenase 1 (Hmox1) genes in the offspring's hippocampus in a model of accelerated senescence during adult life, suggesting that epigenetic modifications may underlie the metabolic effects induced by the high flavonoid intake during gestation [85]. However, as demonstrated by our findings, the alterations induced by maternal naringin supplementation in the offspring's redox status differ according to the brain region, so a given underlying mechanism may be differentially affected according to the developmental stage of each brain region during the period in which the supplementation occurred.
Although the rat and human trajectories of CNS development do not directly correspond, they can be relatively compared when considering the processes that occur during brain development, such as neurogenesis, myelination, synaptogenesis, and synaptic pruning [35,36,86]. As previously discussed, most of the alterations were detected on postnatal day 7, which relatively corresponds to a newborn human when considering brain development [35,36,86]. Moreover, as demonstrated by our findings, the brain regions were differentially affected by maternal naringin supplementation, which might be partially explained by their temporal differences in the developmental processes mentioned above, since such differences also mean distinct neuronal needs and ROS tolerance [35,36,72,86]. However, whether maternal naringin supplementation during pregnancy induces anatomical and behavioral alterations in the offspring, remains a topic for future investigation.
Lastly, as shown in the introduction, the anti-inflammatory and antioxidant properties of polyphenols induced alterations in the closure dynamics of the fetal ductus arteriosus when consumed in high amounts during the third trimester of pregnancy, which is the critical window of development of the fetus' ductus [27,28,87]. Similarly, we have demonstrated that maternal naringin supplementation during the third week of gestation induced redox alterations in the offspring's brain, mainly on postnatal day 7, which promoted a sex-dependent pro-oxidative milieu in the cerebellum and striatum at this postnatal age. Although such alterations did not persist up to weaning, they might be detrimental to brain development, since evidence demonstrates that the neonatal brain is more susceptible to oxidative stress, particularly when further considering that redox alterations during the neonatal period can modify the NSC commitment course, which impairs the neurogenic process and further leads to anatomical and behavioral defects [75,76,78,[88][89][90][91].

Conclusions
We demonstrated for the first time that naringin supplementation during the third week of pregnancy induced region and sex-specific redox alterations in the offspring's brain during postnatal development. Even though maternal naringin consumption positively modulated the pups' brain redox status on postnatal day 1, such effect was not observed on postnatal day 7, in which a pro-oxidative milieu was induced in a sex-dependent manner, thereby suggesting that females and males were specifically affected by the maternal naringin consumption in the cerebellum and striatum, respectively. Differently, the prefrontal cortex and the hippocampus' redox status was positively modulated on postnatal 7. Nevertheless, though naringin induces either a positive or a negative modulation in the offspring's brain redox status, both situations can be detrimental to the NSC commitment process and neurodevelopment. Therefore, future research should focus to unveil the mechanisms underlying the redox alterations induced by maternal naringin supplementation and if such alterations can induce anatomical and behavioral defects related to each of the offspring's brain regions.