In Vitro Tumor Cell Growth Inhibition Induced by Lophocereus marginatus (DC.) S. Arias and Terrazas Endophytic Fungi Extracts
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Isolation and Morphological Characterization of L. marginatus Endophytic Fungi
2.3. Fermentation and Production of Methanol Extracts
2.4. Cell Lines and Culture Conditions
2.5. Effect of Endophytic Fungus Strain Extracts on Murine and Human Tumor Cell Growth
2.6. Molecular Identification of L. marginatus Endophytic fungi
2.7. Statistical Analysis
3. Results
3.1. Isolation of L. marginatus Endophytic Fungi
3.2. Effect of L. marginatus Endophytic Fungi Extracts on Tumor Cell Growth
3.3. Molecular Identification of L. marginatus Endophytic Fungi with Cytotoxic Activity
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Isolate Code | Radial Growth (mm) | Shape | Edge | Mycelium | Topside Color * | Underside Color * | Extract Yield | |
---|---|---|---|---|---|---|---|---|
3 d | 7 d | |||||||
PME-H001 | 7.3 | 9.9 | Circular | Filamentous | Flat | # 838B83 | #F0E68C/EEF3E2 | 5.2% |
PME-H002 | 8.5 | 17.5 | Circular | Filamentous | Flat | #838B83 | #F0E68C/EEF3E2 | 5.2% |
PME-H005 | 8.1 | 17.9 | Circular | Irregular | Flat | #838B83 | #FFC125/FEF0C9 | 12% |
PME-H007 | 6.2 | 22.6 | Circular | Filamentous | Flat | #006400/FFFFFF | #CD9B10/EEDC82 | 8.9% |
PME-H008 | 5.1 | 15.2 | Circular | Entire | Flat | #2F4F4F/EBECE4 | #FEFEF2 | 5.6% |
Isolate Code. | L5178Y-R | HT-29 | MCF-7 | MA-104 | PBMCs | |||||
---|---|---|---|---|---|---|---|---|---|---|
IC50 a | SI * | IC50 | SI | IC50 | SI | IC50 | SI | IC50 | SI | |
PME-H001 | 269.4 ± 1.4 | 1.6/1 b | 348.1 ± 1.1 | 1.2/0.8 | 1387 ± 0.7 | 0.3/0.2 | 437.7 ± 0.8 | 1 | 295.4 ± 1.2 | 1 |
PME-H002 | 266.5 ± 1.4 | 1.1/1.5 | 402.5 ± 1 | 0.7/1 | 1244 ± 0.6 | 0.2/0.3 | 295.4 ± 1.4 | 1 | 409.8 ± 1.2 | 1 |
PME-H005 | 166.2 ± 1.8 | 0.7/1.5 | 291.6 ± 1.2 | 0.4/0.9 | 95.21 ± 1 | 1.2/2.77 | 123.5 ± 1.3 | 1 | 264 ± 1.5 | 1 |
PME-H007 | 132.9 ± 1.5 | 1.8/0.7 | 291.7 ± 1.3 | 0.8/0.7 | 114.7 ± 1.3 | 2.1/1.8 | 245.9 ± 1.9 | 1 | 215.8 ± 1.6 | 1 |
PME-H008 | 101 ± 1.5 | 2.4/2.9 | 301.1 ± 1.2 | 0.8/0.9 | 337.5 ± 1.3 | 0.7/0.8 | 250.2 ± 1.2 | 1 | 298.8 ± 1.4 | 1 |
Isolate Code | Closest Relatives in NCBI | Query Cover | E-Value | Percent Identity | Classification |
---|---|---|---|---|---|
PME-H001 | Penicillium citrinum strain IBB_40 (MH793859.1) | 97% | 0.0 | 99.6% | Penicillium citrinum |
PME-H002 | Penicillium citrinum strain MEBP0016 (MT597829.1) | 100% | 6 × 10−131 | 99.2% | Penicillium citrinum |
PME-H005 | Aspergillus tabacinus (MT635280.1) | 100% | 2 × 10−47 | 99% | Aspergillus sp. |
Aspergillus versicolor strain HM65 (MT609910.1) | 100% | 2 × 10−47 | 99% | ||
PME-H007 | Metarhizium anisopliae isolate CENIEN041 (HQ722915.1) | 98% | 0.0 | 98.9% | Metarhizium anisopliae |
PME-H008 | Cladosporium sp. isolate 978-SAB SA4 2 (MT820353.1) | 98% | 0.0 | 97.6% | Cladosporium sp. |
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Ramírez-Villalobos, J.M.; Romo-Sáenz, C.I.; Morán-Santibañez, K.S.; Tamez-Guerra, P.; Quintanilla-Licea, R.; Orozco-Flores, A.A.; Romero-Arguelles, R.; Tamez-Guerra, R.; Rodríguez-Padilla, C.; Gomez-Flores, R. In Vitro Tumor Cell Growth Inhibition Induced by Lophocereus marginatus (DC.) S. Arias and Terrazas Endophytic Fungi Extracts. Int. J. Environ. Res. Public Health 2021, 18, 9917. https://doi.org/10.3390/ijerph18189917
Ramírez-Villalobos JM, Romo-Sáenz CI, Morán-Santibañez KS, Tamez-Guerra P, Quintanilla-Licea R, Orozco-Flores AA, Romero-Arguelles R, Tamez-Guerra R, Rodríguez-Padilla C, Gomez-Flores R. In Vitro Tumor Cell Growth Inhibition Induced by Lophocereus marginatus (DC.) S. Arias and Terrazas Endophytic Fungi Extracts. International Journal of Environmental Research and Public Health. 2021; 18(18):9917. https://doi.org/10.3390/ijerph18189917
Chicago/Turabian StyleRamírez-Villalobos, Jesica M., César I. Romo-Sáenz, Karla S. Morán-Santibañez, Patricia Tamez-Guerra, Ramiro Quintanilla-Licea, Alonso A. Orozco-Flores, Ricardo Romero-Arguelles, Reyes Tamez-Guerra, Cristina Rodríguez-Padilla, and Ricardo Gomez-Flores. 2021. "In Vitro Tumor Cell Growth Inhibition Induced by Lophocereus marginatus (DC.) S. Arias and Terrazas Endophytic Fungi Extracts" International Journal of Environmental Research and Public Health 18, no. 18: 9917. https://doi.org/10.3390/ijerph18189917