Phenotypic and Genotypic Characterization with MALDI-TOF-MS Based Identification of Staphylococcus spp. Isolated from Mobile Phones with their Antibiotic Susceptibility, Biofilm Formation, and Adhesion Properties

Cell phones, smartphones, and tablets are extensively used in social and professional life, so they are frequently exposed to bacteria. The main goal of the present work was to isolate and characterize Staphylococci strains from students’ cell phone mobiles. Subsequently, 24 Staphylococci strains were tested against a wide range of antibiotics, for the distribution of some virulence-related genes and their ability to form biofilm. Staphylococcus spp. were cultured from all studied devices on chromogenic medium and identified using the matrix-assisted laser desorption/ionization (MALDI), time-of-flight (TOF) mass spectrometry (MS) technique (MALDI-TOF-MS). The results obtained showed that S. aureus was the dominant species (19 strains, 79.1%), followed by S. warneri (3 strains, 12.5%), and S. haemolyticus (2 strains, 8.3%). Isolated strains showed high percentages of hydrolytic enzymes production, resistance to many tested antibiotics, and 37.5% expressed the mecA gene. The tested strains were highly adhesive to polystyrene and glass and expressed implicated icaA (62.5%) and icaD (66.6%) genes. All Staphylococcus spp. strains tested were found to possess proteases and the α-hemolysin gene. Our results highlighted the importance of mobile phones as a great source of Staphylococcus spp., and these species were found to be resistant to many antibiotics with multiple antibiotic resistance (MAR) index ranging from (0.444) to (0.812). Most of the studied strains are able to form biofilm and expressed many virulence genes. Phylogenetic analysis based on the phenotypic and genetic characters highlighted the phenotypic and genetic heterogeneity of the S. aureus population studied. Further analyses are needed to elucidate the human health risks associated with the identified Staphylococci strains.


Introduction
In Tunisia, the first experimental communication made by the global system for mobile telecommunication group was done in 1991 [1]. In fact, more than 77% of the world's population owns a cell phone now [2]. This device has become one of the most indispensable accessories of professional and social life. Its use is considered as an essential part of our everyday professional as well as personal life [3,4]. Nowadays, the cell phone is an essential commodity and represents a health hazard device, because it is often maintained close to human face, ears, hands, and lips [5]. As a result, the cell phone mobiles are a potentially niche of microorganisms that can be dangerous for human health [6]. In fact, the colonization of mobile phone and smartphones by Gram-positive and Gram-negative bacterial strains belonging to Micrococcus, Staphylococcus, Acinetobacter, Klebsiella, Escherichia, Streptococcus, and Pseudomonas genera has been previously reported [5][6][7][8]. It has been also demonstrated that these devices used in hospital environments are frequently associated with multidrug-resistant and pathogenic bacteria [9][10][11].
Staphylococci constitute normal commensal flora of humans and many animals but are considered to be the leading cause of hospital and nosocomial infections [7]. The high pathogenicity is related to their ability to induce resistance against a wide range of antibiotics and adapt to the changes to a variety of environmental conditions [12]. It has been shown that the adaptation processes of Staphylococcus aureus (S. aureus) occur through significant changes in protein composition and metabolomic profiles that allow them to survive and colonize different biotic and abiotic surfaces [13][14][15]. These changes in proteome and metabolome could be explained by the changes in cell wall thickness, cell size, and ultimately colony size on culture plates [16].
Staphylococcus strains are known to be frequently isolated from biofilm developed on various medical devices [17] and mobile phones [18]. The pathogenicity of these bacteria is related to their ability to adhere to host cell-tissue promoted by the release of toxins [19]. In fact, S. aureus produces several hydrolytic enzymes, such as serine protease, cysteine protease, lipase enzymes, and multiple toxins, acting immediately in the area of infection [20,21].
In order to assess the hygiene applied during the use of cell phones in hospital and public environments, the adhesive capacities and the antibiotics resistance of this pathogen must be evaluated [22]. The aim of the current study was to isolate, identify, and characterize Staphylococcus spp. strains from students' cell phones from a teaching Institute in Monastir (Tunisia). We also evaluated the susceptibility of 24 isolates to antibiotics, virulence features, and biofilm formation capacity on materials used in mobile and smartphones industry, i.e., mostly plastic, glass, silicon, and aluminum. The distribution of some virulence-related genes encoding resistance to methicillin, protease (sspA, sspB), lipase (geh), α-hemolysin (hla), and adhesion properties (icaA, icaD, cna, fnbA) in the genome of all S. aureus, S. haemolyticus, and S. warneri isolates was also investigated.

Staphylococcus spp. Morphotypes on CSA
On the two tested media, colonies were about 2 to 3 mm in diameter after 24 h of incubation. The results on Chapman medium were represented by three different morphotypes: 29.5% yellow colonies ( Figure 1A), 6.1% pink colonies, and 64.28% white colonies.

Species Identification with MALDI-TOF-MS and Hydrolytic Enzymes Production
Twenty-four bacterial isolates were confirmed belonging to the Staphylococcus genus using MALDI-TOF-MS technique. Three distinct Staphylococci species were identified: S. aureus (n = 19), S. warneri (n = 3), and S. haemolyticus (n = 2). The detection of coagulase allows the differentiation of species of the genus Staphylococcus. In fact, 43.68% of the isolated strains were coagulase positive. On DNA agar, 64.85% of the strains resulted positive to DNAase enzyme. In addition, most strains (90.26%) were positive for catalase. Most tested strains produced several hydrolytic exoenzymes like amylase (70.83%), caseinase, and gelatinase (45.83%) with significant activity of the enzyme lecithinase (83.33%). Lipase secretion was low (29.16%) compared to other enzymes. On the other hand, 50% of the strains tested were alpha hemolytic and 29.16% were β-hemolytic (Table 2).

Species Identification with MALDI-TOF-MS and Hydrolytic Enzymes Production
Twenty-four bacterial isolates were confirmed belonging to the Staphylococcus genus using MALDI-TOF-MS technique. Three distinct Staphylococci species were identified: S. aureus (n = 19), S. warneri (n = 3), and S. haemolyticus (n = 2). The detection of coagulase allows the differentiation of species of the genus Staphylococcus. In fact, 43.68% of the isolated strains were coagulase positive. On DNA agar, 64.85% of the strains resulted positive to DNAase enzyme. In addition, most strains (90.26%) were positive for catalase. Most tested strains produced several hydrolytic exoenzymes like amylase (70.83%), caseinase, and gelatinase (45.83%) with significant activity of the enzyme lecithinase (83.33%). Lipase secretion was low (29.16%) compared to other enzymes. On the other hand, 50% of the strains tested were alpha hemolytic and 29.16% were β-hemolytic (Table 2). Table 3 summarizes the results of the susceptibility test of the isolates included in this study. The antimicrobials used in this study are those recommended by the Committee of the French Society of the Antibiogram (2017).
It is important to mention that twelve strains were found to be resistant to cefoxitin according to the disk diffusion method. These results were correlated to the results obtained by PCR amplification of the mecA gene, responsible for resistance to methicillin. Nine isolates (26M, 59B, 65C, 66B, 68T, 74M, 78T, 7T, and 39B) expressed this gene showing a band size of 310bp (Table 3).
Krumperman [23] introduced multiple antibiotic resistant (MAR) index analysis in 1983 [23]. In fact, the calculated MARI index values were ranging from 0.444 to 0.812 for the 16 S. aureus strains Int. J. Environ. Res. Public Health 2020, 17, 3761 4 of 17 tested. Interestingly, the S. aureus strains (68T) was resistant to thirteen antibiotics tested with an MAR index value about 0.812. The MARI values ranged from 0.529 to 0.777 for the S. haemolyticus strains and from 0.666 to 0.722 for the two S. warneri strains.
S. aureus S. aureus S. aureus S. aureus S. aureus S. aureus S. aureus S. aureus S. aureus S. aureus S. aureus S. aureus S. aureus S. aureus S. aureus

Adhesive Properties
The capacity of Staphylococcus strains to adhere to mobile phones was estimated both qualitatively and quantitatively. Five morphotypes were defined according the color scale visualized on Conge red agar: non-slime producing Staphylococcus spp. strains are characterized by pink, red, red with black center, and Bordeaux colonies, while black morphotype characterizing slime positive bacteria ( Figure 2). Int. J. Environ. Res. Public Health 2020, 17, x; doi: FOR PEER REVIEW www.mdpi.com/journal/ijerph norfloxacin, and levofloxacin (percentage not exceed 25%). It is important to mention that twelve strains were found to be resistant to cefoxitin according to the disk diffusion method. These results were correlated to the results obtained by PCR amplification of the mecA gene, responsible for resistance to methicillin. Nine isolates (26M, 59B, 65C, 66B, 68T, 74M, 78T, 7T, and 39B) expressed this gene showing a band size of 310bp (Table 3).
Krumperman [23] introduced multiple antibiotic resistant (MAR) index analysis in 1983 [23]. In fact, the calculated MARI index values were ranging from 0.444 to 0.812 for the 16 S. aureus strains tested. Interestingly, the S. aureus strains (68T) was resistant to thirteen antibiotics tested with an MAR index value about 0.812. The MARI values ranged from 0.529 to 0.777 for the S. haemolyticus strains and from 0.666 to 0.722 for the two S. warneri strains.

Adhesive Properties
The capacity of Staphylococcus strains to adhere to mobile phones was estimated both qualitatively and quantitatively. Five morphotypes were defined according the color scale visualized on Conge red agar: non-slime producing Staphylococcus spp. strains are characterized by pink, red, red with black center, and Bordeaux colonies, while black morphotype characterizing slime positive bacteria ( Figure 2). The main results showed that a strain of S. aureus (12C black on CRA) exhibited high biofilm formation on polystyrene (OD = 1.34 ± 0.18) and glass (1.09 ± 0.05) compared to the reference strain S. aureus ATCC 43,300 on polystyrene (OD = 1.89 ± 0.13) and glass (1.48 ± 0.15). Amongst all tested isolates, four (16.66%) displayed positive and variable phenotype on CRA plates, indicating slime production (Table 4). The main results showed that a strain of S. aureus (12C black on CRA) exhibited high biofilm formation on polystyrene (OD = 1.34 ± 0.18) and glass (1.09 ± 0.05) compared to the reference strain S. aureus ATCC 43,300 on polystyrene (OD = 1.89 ± 0.13) and glass (1.48 ± 0.15). Amongst all tested isolates, four (16.66%) displayed positive and variable phenotype on CRA plates, indicating slime production (Table 4).
Qualitative evaluation of biofilm formation potential on glass tubes showed that 58.33% of Staphylococcus isolates were strongly adherent (noted +++) and 29.16% were moderately adherent (noted ++) to this material. Furthermore, the result from 1% crystal violet (CV) staining assay showed that of all isolates tested, only three S. aureus isolates (12C, 67M, and 76C) and one S. haemolyticus strain (39B) were found to be highly adherent to polystyrene showing OD 570 > 1. The strains 12C, 56M, and 67M of S. aureus were highly biofilm forming on glass strips (noted "H") and all other strains were moderately adherent (noted "M") to glass. Two strains of S. aureus (12C and 67M) were highly adherent to polystyrene and glass. All these data are summarized in Table 4.

Detection of Biofilm, Exoenzymes, and Haemolysin Related Genes
According to our results, ten S. aureus strains (52.6%) were positive for both icaA (amplifying a specific 198pb-band) and icaD (amplifying a specific 188pb-band) genes encoding the intracellular adhesion A and B factors, respectively, as compared to the S. aureus ATCC 43,300 positive control strain for all tested genes. Among the virulence factors identified in S. aureus strains, the presence of genes encoding fibronectin (fnbA) and collagen (cna) was emphasized. The can gene encoding the collagen binding protein was detected in the genome of twelve tested bacteria (50.0%), whereas eighteen (75.0%) expressed the fnbA gene. Interestingly, the four biofilm related genes were detected in the genome of eight S. aureus strains (42.1%).
The tested Staphylococcus spp. strains produced several hydrolytic enzymes (Table 2). In fact, four (16.6%) and sixteen (66.6%) strains were positive for sspA and sspB, respectively. In addition, two S. aureus and one S. haemolyticus strains (12.5%) expressed both genes (Table 5). Our results revealed also that eight isolates (33.3%) harbored the geh gene showing a band size of 473 bp. The hla gene encoding hemolysin alpha was detected in nine strains (37.5%) giving a band about 201 bp.

Statistical Analysis
The data from this study were captured, recorded, and analyzed by SPSS 17.0 software. The non-parametric Mann-Whitney U test was used to compare biofilm production assays and the presence of genes. We tested the correlation between biofilm forming Staphylococcus spp. strains on glass and polystyrene material and (exoenzymes/biofilm/hemolysin) related genes ( Table 6). No difference was observed in the prevalence rate of all tested genes and the ability to form a mature biofilm on both glass and polystyrene material. Table 6. Correlation between the ability to form a biofilm on polystyrene and glass material with biofilm related genes (icaA, icaD, cna, fnbA), α-hemolysin (hla), and exoenzymes genes (geh, sspA, sspB). Dendrograms were constructed using the unweighted pair group method of arithmetic averages (UPGMA) and Jaccard's correlation coefficient based on the phenotypic features (exoenzymes produced, susceptibility to antibiotics, and biofilm formation capacity), and/or genetic traits (distribution of some virulence-related genes: mecA; sspA, sspB; geh; hla; icaA, icaD, cna, fnbA). The phylogenetic analysis of the obtained patterns based on both phenotypic and genetic characters has shown that the S. aureus population studied (20 isolates) exhibit high amount of heterogeneity. For a degree of similarity greater than 0.75, we founded the presence of 9 phylogroups ( Figure 3) and 13 genogroups (Figure 4) highlighting the heterogeneity of the S. aureus population studied.

Statistical Analysis
The data from this study were captured, recorded, and analyzed by SPSS 17.0 software. The nonparametric Mann-Whitney U test was used to compare biofilm production assays and the presence of genes. We tested the correlation between biofilm forming Staphylococcus spp. strains on glass and polystyrene material and (exoenzymes/biofilm/hemolysin) related genes (Table 6). No difference was observed in the prevalence rate of all tested genes and the ability to form a mature biofilm on both glass and polystyrene material. Table 6. Correlation between the ability to form a biofilm on polystyrene and glass material with biofilm related genes (icaA, icaD, cna, fnbA), α-hemolysin (hla), and exoenzymes genes (geh, sspA, sspB). Dendrograms were constructed using the unweighted pair group method of arithmetic averages (UPGMA) and Jaccard's correlation coefficient based on the phenotypic features (exoenzymes produced, susceptibility to antibiotics, and biofilm formation capacity), and/or genetic traits (distribution of some virulence-related genes: mecA; sspA, sspB; geh; hla; icaA, icaD, cna, fnbA). The phylogenetic analysis of the obtained patterns based on both phenotypic and genetic characters has shown that the S. aureus population studied (20 isolates) exhibit high amount of heterogeneity. For a degree of similarity greater than 0.75, we founded the presence of 9 phylogroups ( Figure 3) and 13 genogroups (Figure 4) highlighting the heterogeneity of the S. aureus population studied.   The high variability in the patterns obtained based on the phenotypic and genetic features clustered together (for a degree of similarity greater than 0.75, we found the presence of 14 clusters) highlighted the high diversity in the S. aureus population from cell phones ( Figure 5).  The high variability in the patterns obtained based on the phenotypic and genetic features clustered together (for a degree of similarity greater than 0.75, we found the presence of 14 clusters) highlighted the high diversity in the S. aureus population from cell phones ( Figure 5).  The high variability in the patterns obtained based on the phenotypic and genetic features clustered together (for a degree of similarity greater than 0.75, we found the presence of 14 clusters) highlighted the high diversity in the S. aureus population from cell phones ( Figure 5).

Discussion
The misuse of smartphones, especially cell phones and the lack of safety hygiene practices, make them a potent source of pathogenic microorganisms. Furthermore, it has been demonstrated that humidity and temperature are the main environmental factors known to affect the biodiversity of microorganisms colonizing cell phones [24]. Through every phone call, there is a risk of bacterial contamination to different parts of the human body accentuated by hand-to-facial contact (lips and ears) or by hand to the surrounding surface [25][26][27][28][29]. In fact, the percentage of contaminated cell phones in the studies made by Tagore et al. [26], Bhat et al. [6], Ulger et al. [28], and Badr et al. [29] were 100%, 99%, 94.5%, and 93.7%, respectively.
Several studies from different parts in India showed that the predominant organisms isolated from contaminated cell phones are Coagulase Negative Staphylococci (CoNS) followed by Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Acinetobacter sp, Enterococcus faecalis, and Pseudomonas aeruginosa [30,31]. Additionally, other microorganisms have been reported to be the main harmful bacteria isolated from health professional's mobile phones in a tertiary care hospital of eastern part of Bengal including S. epidermidis, Micrococcus sp., B. subtilis, diphtheroids, E. faecalis, α-hemolytic streptococci, and non-fermenter Gram-negative coccobacilli [32]. Interestingly, strains with high resistance to antibiotics, especially to methicillin, aminoglycoside, carbapenem, and Extended-spectrum beta-lactamases, or ESBLs, producing organisms were also identified [33].
Our results indicated that the majority of the tested isolates were resistant to the tested antibiotics (MARI ranging from 0.444 to 0.812) including those routinely used to treat infection due to the S. aureus species. The same results have previously been reported in China and Russia [34,35]. Interestingly, the tested S. aureus strains identified in the present study were resistant to erythromycin (79.16%). Similar results have been observed in China (97.8%) [36], United Kingdom (90%) [37], and Australia (98%) [38]. As compared to previous studies, our identified Staphylococci strains were highly resistant to tetracycline (87.5%), whereas it was 48% and 44% in Lebanon [39,40] and (5%) in the USA [41].
Antibiotic sensitivity patterns of the isolates showed that 21.05% of S. aureus were resistant to methicillin (MRSA). Different studies reported the incidence of MRSA isolated from cell phones was variable in different geographical areas like 16.9% in Mumbai [42], 52.4% in Bhabnagar [43], 52% in Turkey [28], and only 12% in Iran [44].
Clinical S. aureus species produces several types of secreted exoenzymes such as hemolysins and adhesins [45][46][47][48][49][50]. This bacterium is known to produce about twenty-three Staphylococcal Enterotoxins (SEs) and Enterotoxin-like toxins (SEls) responsible for its pathogenic character and are frequently associated to food poisoning and toxic shock syndrome [51]. Indeed, S. aureus species is known to produce several exotoxins like staphylokinase, lipases, proteases, collagenases, hemolysins, exfoliative toxins, and superantigen proteins. Many species other than S. aureus including S. cohnii, S. epidermis, S. xylosus, S. haemolyticus, S. hyicus, and S. intermedius produce the same virulence factors [52]. This bacterium produces pigmented colonies on Congo red agar and has the ability to colonize, adhere, and form a mature biofilm on both biotic and abiotic surfaces [53][54][55].
The detection of the virulence-related genes in the genome of the identified S. aureus, S. haemolyticus, and S. warneri strains using PCR technique showed the presence of icaA gene in 11/19 S. aureus strains (57.89%). Similar results have been reported by Arciola et al. [55] suggesting that 60.86% of S. aureus strains harbor icaA and icaD genes. Whereas, Rohde et al. [56] found that all isolated S. aureus have the icaA gene. Additionally, icaA/icaD genes were found in the genome of 10/19 S. aureus strains (52.63%) including non-slime producing bacteria on CRA. While, Arciola et al. [55] suggested that icaA + /icaD + pattern was detectable only in slime-producing strains.
Seven S. aureus strains (36.84%) were positive for the three adhesins genes (icaD, fnbA, and cna). One S. Haemolyticus strain (39B) was positive for two genes (fnbA, cna). These results were in accordance of those reported by Arciola et al. [57] who reported that 84/191 clinical S. aureus strains (44%) harbored two adhesin genes (fnbA and cna) and only 0.5% of them were positive for cna gene. We also noted that 75% of the tested strains were positive for the fnbA gene. In fact, Arciola et al. [57] mentioned that 98.4% of S. aureus strains isolated from infections associated to implant devices were fnbA+ and 99.5% of them were fnbB+.
In the present work, the cna gene was detected in 54.16% of the identified Staphylococci. A previous report demonstrated that the distribution of this gene is related to the area of study and the methodological approach used [58,59]. Additionally, we found that nine isolates were positive for mecA gene (37.5%) responsible of methicillin-resistance including one S. haemolyticus (7T) and one S. warneri strain (30C).
It is well known that a wide range of staphylococcal species harbor the mecA gene encoding an alternative penicillin-binding protein 2a [60][61][62][63]. Interestingly, Bouchami et al. [64] reported the amplification of the mecA gene in 45 out of 49 (91.8%) clinical bacteremic CoNS isolates (43 S. epidermidis, and 2 S. haemolyticus) collected in the bone marrow transplant center of Tunisia from 1998 to 2007. In addition, it was demonstrated that the small-size SCCmec types harboring the mecA gene are mobile and circulate in the environment leading to the spread of mecA and the rise in nosocomial methicillin-resistant staphylococcal infections [65].
In previous works, it was demonstrated that the mecA gene is transferred from coagulase-negative staphylococcal species to S. aureus in vivo explicating the emergence of more successful S. aureus clones with high adherence/invasion capacities [66,67]. In fact, Harisson et al. [68] identified two Staphylococcus sciuri subsp. carnaticus isolates from bovine infections that harbor three different mecA homologues: mecA, mecA1, and mecC. Additionally, a novel allele of mecA, namely, mecC (originally mecALGA251, with 70% of similitude with mecA) was identified in MRSA across Europe from livestock, small mammals, and birds. It was also demonstrated that a circulation of this gene between livestock and humans suggesting a zoonotic reservoir for the human isolates [68,69].
The presence of the Staphylococci species isolated on smartphone devices can be explicated by the high incidence of these germs in toilets that can be circulated to the surrounding environment as reported by Mkrtchyan et al. [53]. The same authors founded that 37.8% of these isolates were resistant to antimicrobial agents. Another big issue discussed by Bhoonderowa et al. [54] confirmed that sharing mobile phones among females was associated with high bacterial load.

Sample Collection and Bacteriological Analysis
From February 2017 to May 2017, a survey enrolled 100 students from the High Institute of Biotechnology (Monastir, Tunisia) accepted to be included in the study and gave the consent to analyze their cell phones (n = 100). Participants' ages ranged from 19 to 24 years (M = 20.898, SD = 1.508) with 95% identified as female (n = 95) and 5% as male (n = 5).
A sterile cotton swab was used to analyze the microflora by drawing horizontal then vertical streaks on the keys or the touch screen, and the cover (plastic, silicone, glass) of the mobile phone. All swabs were kept in brain heart infusion (Biolife, Italy) as transport medium and incubated aerobically at 37 • C for 24 h, then cultured on Chapman (Biolife, Italy) and CHROMagar TM Staph (CHROMagar Microbiology, Paris, France) agar Petri dishes at 37 • C for 24 h. Bacterial strains growing on the selective media plates were purified on tryptic soy agar supplemented (TSA, Difco, Spain) plates and subjected to standard morphological (Gram test, catalase, oxidase) biochemical plate or tube tests (coagulase, DNase and mannitol-motility tests). Selected microorganisms were tested for hemolysin on human blood agar (BIO-RAD, France). The enzymes amylase, caseinase, lipase, gelatinase, and lecithinase were detected on media prepared with phosphate buffer saline (PBS) supplemented, respectively, with 0.5% casein peptone, 5% skim milk powder, 1% Tween 80, 5% gelatin powder, and egg yolk emulsion [70]. Strain identification was performed using the MALDI-TOF technique [71].

Susceptibility to Antibiotics
Nineteen antimicrobials agents were tested using the disk diffusion method. A cotton swab was soaked with the bacterial suspension adjusted to 10 7 CFU/mL (OD 600 = 0.1) and streaked across the surface of Mueller-Hinton agar medium. Square Petri dishes were dried for five minutes and the following antibiotic disks ( To interpret the results obtained, we used the multiple antibiotic resistance MAR index defined as a/b, where a represents the number of multiple antibiotics to which the particular isolate is resistant, and b is the number of multiple antibiotics to which the particular isolates were exposed [23,72].

Adhesive Properties of the Identified Bacteria
All identified Staphylococcus spp. strains (n = 24) were tested qualitatively for their ability to produce exopolysaccharides (Slime) on Congo red agar (CRA) medium and safranin (1%) [73,74]. The bacteria-producing slime on CRA plate gave black colonies with a rough surface against red colonies with a smooth surface for non-producing strains. The safranin technique was conducted according to the technique described for coagulase-negative Staphylococci. Briefly, a few colonies of each strain grown on nutrient agar were inoculated into tubes containing 10 mL of Luria Bertani broth supplemented with glucose (final concentration 8%). The tubes were incubated for 24 h at 37 • C and then examined for the presence or absence of membrane formation after the addition of a solution of safranin 1%. The presence of a viscid slime layer was considered as slime producer bacteria. Slime production was scored as negative, weak (+), moderate (++), or strong (+++).
The quantitative analysis of the adhesion ability of the identified Staphylococcus spp. strains was tested against two materials that are used for the construction of cell phones: polystyrene and glass using the crystal violet technique [70].
All Staphylococcus spp. strains were tested for their ability to form a biofilm on polystyrene 96 well tissue culture plates (Nunc, Roskilde, Denmark) as previously described [75,76]. Two type strains namely S. aureus ATCC 25,923 and S. aureus ATCC 43,300 were used as positive controls. Biofilm formation was categorized as highly positive (OD 570 ≥ 1), low-grade positive (0.1 ≤ OD 570 < 1), or negative (OD 570 < 0.1). Strips (1.5 cm 2 ) were used for the biofilm formation assay on glass material using the protocol described by da Silva Meira [77]. For the bacterial DNA extraction, the inoculated bacterial strains of S. aureus were incubating for 18 to 24 h at 37 • C in nutrient broth, pure colonies were suspended in 1 mL of a solution of Tris-EDTA (TE), followed by a centrifugal washing step of this suspension (13,200 rpm, 5 min at 4 • C). Subsequently, the supernatant was removed while the pellet was suspended in a volume of 200 µL TE, vortexed, and then heated at 95 • C for 10 min. After incubation, a final centrifugation step (13,200 rpm, 5 min at 4 • C) was made and the supernatant containing the bacterial DNA was moved into new Eppendorf tubes. The DNA was stored at −20 • C until use.
The detection of the mecA gene responsible for methicillin resistance was carried out according to the technique described by Geha et al. [78]. The presence of protease (sspA, sspB), lipase (geh), β-hemolysin (hla), and adhesion (icaA, icaD, can, fnbA) genes in the genome of the tested strains was also detected by using the protocol described by Karlsson et al. [79]. The hla gene was detected using the protocol described by Liang et al. [59]. All primers used, PCR conditions, and amplicon size are reported in Table 7. Table 7. Selected specific primers and PCR-amplification conditions. hla-R 5 -CTGGTGAAAACCCTGAAGATAATAGAG-3

Data Analysis
Phylogenetic trees based on phenotypic and/or genetic traits were constructed using the unweighted pair group method of arithmetic averages (UPGMAs) and Jaccard's correlation coefficient [82]. The estimation of biofilm capacity formation on polystyrene and glass surfaces was conducted in triplicates for each isolate. Mean and standard deviation values were performed with SPSS 17.0 software. The non-parametric Mann-Whitney U test was used to correlate between the quantitative biofilm production assays and the distribution of (exoenzymes/biofilm/hemolysin) related genes.

Conclusions
Our study demonstrably highlighted that many Staphylococci species can be associated with smartphones of students. Most of these bacteria produce several virulence-related factors that allow them to adhere and form a mature biofilm on these devices. Thus, probably allowing to them to be transferred to the organs coming in deep contact with the cell phone. Interestingly, the mecA gene, responsible for resistance to methicillin was amplified in two coagulase-negative Staphylococcus species (S. haemolyticus and S. warneri) leading to the emergence of new clones of drug resistant bacteria. The lack of awareness of using cell phones may contribute to a significant risk of transmitting multidrug-resistant bacteria through unguarded cell phone use. New biomaterial used for smartphone manufacturing or decontamination solution have to be designed to avoid the contamination by these bacteria.