Newly Designed Hydrolysis Acidification Flat-Sheet Ceramic Membrane Bioreactor for Treating High-Strength Dyeing Wastewater

Cost-effective treatment of dyeing wastewater remains a challenge. In this study, a newly designed hydrolysis acidification flat-sheet ceramic membrane bioreactor (HA-CMBR) was used in treating high-strength dyeing wastewater. The start-up phase of the HA-CMBR was accomplished in 29 days by using cultivated seed sludge. Chemical oxygen demand (COD) removal rate reached about 62% with influent COD of 7800 mg/L and an organic loading rate of 7.80 kg-COD/(m3·d). Chromaticity removal exceeded 99%. The results show that the HA-CMBR has good removal performance in treating dyeing wastewater. The HA-CMBR could run with low energy consumption at trans-membrane pressure (TMP) <10 kPa due to the good water permeability of the flat-sheet ceramic membrane. New strains with 92%–96% similarity to Alkalibaculum bacchi, Pseudomonas sp., Desulfovibrio sp., and Halothiobacillaceae were identified in the HA-CMBR. Microbial population analysis indicated that Desulfovibrio sp., Deltaproteobacteria, Halothiobacillaceae, Alkalibaculum sp., Pseudomonas sp., Desulfomicrobium sp., and Chlorobaculum sp. dominated in the HA-CMBR.


Introduction
China is the world's largest producer of dyes, at more than 1.15 × 10 9 kg annually [1]. Dye exports have brought some economic growth for China, but the dyeing production process generates large volumes of dyeing wastewater. The raw materials of dyeing production include benzene, naphthalene, and polycyclic aromatic hydrocarbons, but also contain heavy metals, salts, and other substances. Therefore, dyeing wastewater is characterized by complex compositions, deep color, and toxicity for living systems [2]. The discharge of untreated dyeing wastewater causes serious pollution to surrounding environments.
The cost-efficient treatment of dyeing wastewater has attracted much research interest [3][4][5]. At present, physical and chemical methods are usually used to treat dyeing wastewater [6]. However, such methods have high operating costs. In comparison, biological methods have the advantages of low running costs and being more environmentally friendly, and are expected to be widely used in treating dyeing wastewater [7]. Li et al. [8] reinforced an anaerobic hydrolysis-denitrification coupling process, and the coupling process showed efficient removal of nitrogen and aromatics. Hayat et al. [9] achieved 87% chemical oxygen demand (COD) removal efficiency in an anaerobic internal circulation (IC) reactor treating real textile industry wastewater. Manavi et al. [10] used aerobic granules to treat

Wastewater Compositions
The wastewater sample used in this study was obtained from Weifang Ruicheng company (Shandong, China) that produces disperse, reactive, and acid dyes. The compositions of the dyeing wastewater are shown in Table 1. Due to the lack of nitrogen and phosphorus nutrients in the dyeing wastewater, NH 4 HCO 3 and KH 2 PO 4 are added as nitrogen and phosphorus nutrients in the required proportion.

Reactors
A schematic diagram of an HA-CMBR system is described by Zhang et al. [12]. Anaerobic microorganisms from a running hydrolysis acidification tank [17] were seeded in the main reactor. The permeate was discharged into the effluent tank using a filtration pump. The feed flow rate of HA-CMBR was 16 L/d with an HRT of 12 h. The HA-CMBR consisted of an influent tank, main reactor, warm water control system, and effluent tank. The influent was adjusted for pH in the influent tank and pumped into the bottom of the main reactor. The temperature of the main reactor and membrane pool was controlled at 34 ± 1 • C. The HA-CMBR was seeded with 2.5 L of sludge that had a mixed liquor volatile suspended solids (MLVSS) content of 10,000 mg/L.

Measurement Methods
Filtered COD (1 µm) based on dichromate was measured using the closed reflux colorimetric method [18]. Biochemical oxygen demand over a five-day (BOD 5 ) and chroma were measured according to the standard methods [19]. Total nitrogen (TN) was determined with the persulfate method [20] using the ultraviolet spectrophotometric screening method for quantification of TN as NO 3 -N (i.e., oxidization product of persulfate digestion). Total phosphorus (TP) was measured according to Yue et al. [21,22]. The pH was measured using a pH meter (9010; Jenco, San Diego, CA, USA), and dissolved oxygen (DO) was measured using a DO meter (6010; Jenco, San Diego, CA, USA). Trans-membrane pressure (TMP) was recorded by a digital pressure sensor (SHANG YI, Foshan, China). Extracellular polymeric substances (EPS) and soluble microbial products (SMP) were analyzed according to Ramesh et al. [23].

Reactor Start-Up and Operation Parameters
The HA-CMBR was fitted with a flat-sheet ceramic membrane of normal pore size 0.1 µm and effective area 0.05 m 2 . The hydraulic residence time was set to 8 h (according to the main reactor). The main reactor was seeded with 3 L sludge that had a mixed liquor volatile suspended solids (MLVSS) content of 8000 mg/L.

Data Analysis
Data analysis was carried out using Origin 2017 software (OriginLab, Northampton, MA, USA). To explore the correlation between microbial diversity and environmental factors, detrended correlation analysis was performed on the species.

Reactor Performance
Diluted dyeing wastewater with COD concentration of 1300-1349 mg/L was used for the start-up of the HA-CMBR. The OLR was set at 1.3-1.5 kg-COD/m 3 /d. During the first three days of start-up, COD removal efficiency was poor with an effluent COD concentration of about 900 mg/L, after which the effluent COD gradually decreased during the following 22 days. A COD removal rate of 65% was achieved on day 23. From day 23, the COD removal rate remained stable in the following six days. Thus, the startup of HA-CMBR was deemed to be accomplished in 29 days. During the start-up period, a good chroma removal rate of 99% was achieved, which indicates that the microorganisms in the seed sludge are competent in decolorizing the dyeing wastewater.
After treatment, the average BOD 5 /COD of stages I-VI increased from 0.04 to 0.39, 0.27, 0.24, 0.27, 0.23, and 0.18, respectively ( Figure 1). The experimental data indicate that the biodegradability of the dyeing wastewater improved significantly following treatment by the HA-CMBR. Similar to the COD removal performance, at stage V and VI, average BOD 5 /COD decreased by approximately 50%.
The results indicate that anaerobic microorganisms were inhibited during stages V and VI. up, COD removal efficiency was poor with an effluent COD concentration of about 900 mg/L, after which the effluent COD gradually decreased during the following 22 days. A COD removal rate of 65% was achieved on day 23. From day 23, the COD removal rate remained stable in the following six days. Thus, the startup of HA-CMBR was deemed to be accomplished in 29 days. During the startup period, a good chroma removal rate of 99% was achieved, which indicates that the microorganisms in the seed sludge are competent in decolorizing the dyeing wastewater.
After treatment, the average BOD5/COD of stages I-VI increased from 0.04 to 0.39, 0.27, 0.24, 0.27, 0.23, and 0.18, respectively ( Figure 1). The experimental data indicate that the biodegradability of the dyeing wastewater improved significantly following treatment by the HA-CMBR. Similar to the COD removal performance, at stage V and VI, average BOD5/COD decreased by approximately 50%. The results indicate that anaerobic microorganisms were inhibited during stages V and VI. Influent chroma and the total chroma removal rate were approximately 1000 and 99%, respectively, in stage I; 2000-2500 and 99.6% in stage II; 2000-2500 and 99.6% in stage III; 3500 and 99.7% in stage IV; 4000 and 99.8% in stage V; and 5000 and 99.5% in stage VI ( Figure 2). Chroma removal rates exceeded 99%. During the first four stages, the influent color changed from navy blue to almost white. After filtration through the flat-sheet ceramic membrane, the effluent turned clear, and no obvious color could be identified. Although the chroma removal rates were slightly decreased in stages V and VI, the effluent chroma still met the relevant discharge standards. The results show that the HA-CMBR has good chroma removal performance in treating the dyeing wastewater used in this study. The average TN and TP removal rates were 20% and 6% in the HA-CMBR, which are similar to the results reported by Jin et al. [24]. DO was controlled below 0.5 mg/L. pH was maintained at 7.0-8.0. Influent chroma and the total chroma removal rate were approximately 1000 and 99%, respectively, in stage I; 2000-2500 and 99.6% in stage II; 2000-2500 and 99.6% in stage III; 3500 and 99.7% in stage IV; 4000 and 99.8% in stage V; and 5000 and 99.5% in stage VI ( Figure 2). Chroma removal rates exceeded 99%. During the first four stages, the influent color changed from navy blue to almost white. After filtration through the flat-sheet ceramic membrane, the effluent turned clear, and no obvious color could be identified. Although the chroma removal rates were slightly decreased in stages V and VI, the effluent chroma still met the relevant discharge standards. The results show that the HA-CMBR has good chroma removal performance in treating the dyeing wastewater used in this study. The average TN and TP removal rates were 20% and 6% in the HA-CMBR, which are similar to the results reported by Jin et al. [24]. DO was controlled below 0.5 mg/L. pH was maintained at 7.0-8.0.

Membrane Fouling
During the study, the membrane flux was set at 5 L/m 2 /h. As mentioned previously, the test was divided into six stages. At the end of each phase, the flat-sheet ceramic membranes were chemically cleaned by pumping 1000 mg/L NaOCl into the inner space of the membranes. The membranes were soaked for 1-2 h to recover filtration performance. Figure 3 shows the TMP, SMP, and EPS changes during the study. TMP increased from 1.7 to 8.3 kPa in stage I (488%); 1.8 to 7.2 kPa in stage II (400%); 1.8 to 5.9 kPa in stage III (328%); 1.7 to 8.5 kPa in stage IV (500%); 1.8 to 5.9 kPa in stage V (328%); and from 1.6 to 7.4 kPa in stage VI (462%). The results show that the TMP of the flat-sheet ceramic membrane was restored to the initial value after chemical cleaning.

Membrane Fouling
During the study, the membrane flux was set at 5 L/m 2 /h. As mentioned previously, the test was divided into six stages. At the end of each phase, the flat-sheet ceramic membranes were chemically cleaned by pumping 1000 mg/L NaOCl into the inner space of the membranes. The membranes were soaked for 1-2 h to recover filtration performance. Figure 3 shows the TMP, SMP, and EPS changes during the study. TMP increased from 1.7 to 8.3 kPa in stage I (488%); 1.8 to 7.2 kPa in stage II (400%); 1.8 to 5.9 kPa in stage III (328%); 1.7 to 8.5 kPa in stage IV (500%); 1.8 to 5.9 kPa in stage V (328%); and from 1.6 to 7.4 kPa in stage VI (462%). The results show that the TMP of the flat-sheet ceramic membrane was restored to the initial value after chemical cleaning.
EPS and SMP easily adhere to the surface of the membrane, thereby clogging the membrane pores [12,14]. As shown in Figure 3, the EPS and SMP concentrations were closely related to TMP. From stage I to stage III, there were decreasing concentrations of EPS and SMP. Correspondingly, the TMP at the end of each phase decreased from 8.3 kPa to 5.9 kPa. In stage IV, the concentrations of EPS and SMP increased, as did TMP. Thus, TMP increased with decreasing membrane flux. Zhang et al. [12] also found that the decrease in membrane flux was mainly caused by a combination of EPS, SMP, and fine sludge particles attached to the membrane pores and surface. Beyond that, there were subsequent decreases in EPS and SMP concentration during stages V and VI. EPS and SMP were found to be positively related to microbial activity [14]. During stages V and VI, microbial inhibition led to poor COD removal rates and therefore decreasing EPS and SMP. The results show that membrane fouling in this study occurred mainly due to EPS and SMP. soaked for 1-2 h to recover filtration performance. Figure 3 shows the TMP, SMP, and EPS changes during the study. TMP increased from 1.7 to 8.3 kPa in stage I (488%); 1.8 to 7.2 kPa in stage II (400%); 1.8 to 5.9 kPa in stage III (328%); 1.7 to 8.5 kPa in stage IV (500%); 1.8 to 5.9 kPa in stage V (328%); and from 1.6 to 7.4 kPa in stage VI (462%). The results show that the TMP of the flat-sheet ceramic membrane was restored to the initial value after chemical cleaning.

Microbial Diversity
At the end of the start-up period, microbial diversity showed an obvious change. The seed sludge showed high microbial diversity, however, after treating the dyeing wastewater (see Figure 4) EPS and SMP easily adhere to the surface of the membrane, thereby clogging the membrane pores [12,14]. As shown in Figure 3, the EPS and SMP concentrations were closely related to TMP. From stage I to stage III, there were decreasing concentrations of EPS and SMP. Correspondingly, the TMP at the end of each phase decreased from 8.3 kPa to 5.9 kPa. In stage IV, the concentrations of EPS and SMP increased, as did TMP. Thus, TMP increased with decreasing membrane flux. Zhang et al. [12] also found that the decrease in membrane flux was mainly caused by a combination of EPS, SMP, and fine sludge particles attached to the membrane pores and surface. Beyond that, there were subsequent decreases in EPS and SMP concentration during stages V and VI. EPS and SMP were found to be positively related to microbial activity [14]. During stages V and VI, microbial inhibition led to poor COD removal rates and therefore decreasing EPS and SMP. The results show that membrane fouling in this study occurred mainly due to EPS and SMP.

Microbial Diversity
At the end of the start-up period, microbial diversity showed an obvious change. The seed sludge showed high microbial diversity, however, after treating the dyeing wastewater (see Figure  4), bands 1, 3, 4, 6, 7, and 10 disappeared, and new bands 2, 12, 14, and 15 dominated in the HA-CMBR. Bands 5,9,11,13, and 16 were further enriched with increasing NLRs (Nos. 4-8), while bands 2, 8, 15, and 17 decreased. The present findings show that bands 5,9,11,13,14, and 16 can dominate in treating dyeing wastewater. To evaluate the relationships between the bacteria in the sludge, a microbial phylogenetic tree was built as shown in Figure 5. The identified bands can be clustered into eight genera: Desulfovibrio sp., Desulfomicrobium sp., Chlorobaculum sp., Chlorobium sp., Pseudomonas sp., Halothiobacillaceae, To evaluate the relationships between the bacteria in the sludge, a microbial phylogenetic tree was built as shown in Figure 5. The identified bands can be clustered into eight genera: Desulfovibrio sp., Desulfomicrobium sp., Chlorobaculum sp., Chlorobium sp., Pseudomonas sp., Halothiobacillaceae, Clostridium sp., and Alkalibaculum sp. Desulfovibrio sp. was reported to have the potential to degrade both Reactive Black 5 and Remazol Brilliant Blue R under hydrolysis acidification conditions, to deal with simulated dyeing wastewater [25]. Li et al. [26] found that Desulfomicrobium sp. was abundant in anaerobic sludge used for synergetic decolorization of Reactive Blue 13 effluent.
Chlorobium sp. was reported to be dominant in mature biofilm when treating real dyeing wastewater [27]. Pseudomonas sp. was identified in a down flow microaerophilic fixed film bioreactor treating reactive azo dyes [28]. Halothiobacillaceae was detected in a sequencing batch reactor for cloth printing and dyeing wastewater treatment [29]. Clostridium sp. was reported to achieve complete decolorization of Remazol reactive dyes after 24-72 h [30]. Alkalibaculum sp. was reported to participate in the degradation of complex organic matter, such as lignocellulosic wastes [31]. Clostridium sp., and Alkalibaculum sp. Desulfovibrio sp. was reported to have the potential to degrade both Reactive Black 5 and Remazol Brilliant Blue R under hydrolysis acidification conditions, to deal with simulated dyeing wastewater [25]. Li et al. [26] found that Desulfomicrobium sp. was abundant in anaerobic sludge used for synergetic decolorization of Reactive Blue 13 effluent. Chlorobium sp. was reported to be dominant in mature biofilm when treating real dyeing wastewater [27]. Pseudomonas sp. was identified in a down flow microaerophilic fixed film bioreactor treating reactive azo dyes [28]. Halothiobacillaceae was detected in a sequencing batch reactor for cloth printing and dyeing wastewater treatment [29]. Clostridium sp. was reported to achieve complete decolorization of Remazol reactive dyes after 24-72 h [30]. Alkalibaculum sp. was reported to participate in the degradation of complex organic matter, such as lignocellulosic wastes [31]. The relative abundances of bacterial groups (at the phylum level) in the sludge samples are shown in Figure 6. Following the increasing NLRs, Desulfovibrio sp., Deltaproteobacteria, Halothiobacillaceae, Alkalibaculum sp., Pseudomonas sp., Desulfomicrobium sp., and Chlorobaculum sp. The relative abundances of bacterial groups (at the phylum level) in the sludge samples are shown in Figure 6. Following the increasing NLRs, Desulfovibrio sp., Deltaproteobacteria, Halothiobacillaceae, Alkalibaculum sp., Pseudomonas sp., Desulfomicrobium sp., and Chlorobaculum sp. accounted for approximately 70% of the number of individuals, of which Desulfovibrio sp. occupied the largest share. In sludge sample No. 4, the share of Desulfovibrio sp. was about 50%. Although the share of Desulfovibrio sp. decreased in sludge samples 5 and 6, it still accounted for approximately 30% on average. In sludge samples 5-8, Deltaproteobacteria accounted for 20% on average. The results indicate that Desulfovibrio sp. and Deltaproteobacteria were the most important phyla in the HA-CMBR system used for treating dyeing wastewater in this study. Those phyla with a share of less than 1% are classified as other and together accounted for 30% on average, which means that the sludges were in abundance of biological diversity.

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The map clustering tree based on DGGE is shown in Figure 7. In this tree, if a species in a 189 community becomes a species with high similarity in another community, it is considered a small  The map clustering tree based on DGGE is shown in Figure 7. In this tree, if a species in a community becomes a species with high similarity in another community, it is considered a small change, and the distance between the two communities is short. On the other hand, if a species in a community becomes another species with a low relationship, there is a large distance between the two communities. There was only 37%-43% similarity between seed sludges and the cultivated sludge sampled during the start-up period, which means that almost 60% of species evolved with the dyeing wastewater in this study. Following the increasing NLRs, the similarity increased. There was 73% similarity between sample Nos. 6 and 8, which further increased to 77% between Nos. 5 and 7.
The results indicate that the bacteria in the seed sludge were able to rapidly adapt to the changing feed.
Microbial diversity was analyzed using the Shannon-Weaver index (see Table 2). The diversity indexes of sample Nos. 1 and 2 are 1.412 and 2.325, respectively. Nos. 3-8 have low diversity indexes of 1.935-1.999. No. 1 was collected from a running reactor in the lab, and No. 2 was collected from a real dyeing wastewater treatment plant. The results show that the seed sludge from the real wastewater treatment plant had high microbial diversity. However, following cultivation, microbial diversity decreased, indicating that certain species became dominant at the expense of overall diversity, as shown in Figure 6. Microbial diversity was analyzed using the Shannon-Weaver index (see Table 2). The diversity indexes of sample Nos. 1 and 2 are 1.412 and 2.325, respectively. Nos. 3-8 have low diversity indexes of 1.935-1.999. No. 1 was collected from a running reactor in the lab, and No. 2 was collected from a real dyeing wastewater treatment plant. The results show that the seed sludge from the real wastewater treatment plant had high microbial diversity. However, following cultivation, microbial diversity decreased, indicating that certain species became dominant at the expense of overall diversity, as shown in Figure 6. In this study, good chroma removal is deduced to derive from the new strains developed in the HA-CMBR. There are many types of dyes, potentially requiring different types of microorganisms for decolorization. However, in many cases, microbial information is lacking, which is one of the difficulties faced in biodegradation of dyeing wastewater. In this study, microbes that were not contained in the seed gradually emerged and were further enriched after cultivation. This indicates that the bacterial population in the sludge can be changed and used for treating new types of dye wastewater through efficient cultivation. An HA-CMBR can reduce sludge loss by 100%, which is an efficient means of sludge enrichment. Therefore, HA-CMBR in this study is of practical value in treating dyeing wastewater.  In this study, good chroma removal is deduced to derive from the new strains developed in the HA-CMBR. There are many types of dyes, potentially requiring different types of microorganisms for decolorization. However, in many cases, microbial information is lacking, which is one of the difficulties faced in biodegradation of dyeing wastewater. In this study, microbes that were not contained in the seed gradually emerged and were further enriched after cultivation. This indicates that the bacterial population in the sludge can be changed and used for treating new types of dye wastewater through efficient cultivation. An HA-CMBR can reduce sludge loss by 100%, which is an efficient means of sludge enrichment. Therefore, HA-CMBR in this study is of practical value in treating dyeing wastewater.

Conclusions
HA-CMBR was used in treating dyeing wastewater. After seven days of operation, the COD removal rate reached approximately 64%, and the chroma removal rate was as high as 99%. COD removal rate decreased sharply when influent COD concentration exceeded 9000 mg/L. The microbial community in the sludge samples showed obvious changes, and the main strains in the HA-CMBR were Desulfovibrio sp. (24%), Deltaproteobacteria (15%), and Turicibacter sp. (37%).
The tested AnMBR demonstrates energy-efficient treatment of dye wastewater, and might further enable treatment of different effluent types (through rapidly evolving microbial community structure) and avoidance of biogas emissions/remediation costs.