Ethanol and Acetic Acid Production from Carbon Monoxide in a Clostridium Strain in Batch and Continuous Gas-Fed Bioreactors
Abstract
:1. Introduction
2. Experimental Section
2.1. Microorganism
2.2. Bioconversion Studies
2.2.1. Bottle Batch Experiments
Run No | NH4Cl | Trypticase | YE | Ethanol (g/L) | Acetic Acid (g/L) | Biomass (mg/L) | Final pH |
---|---|---|---|---|---|---|---|
1 | 0.2 | 0.2 | 0.10 | 0.1733 | 1.806 | 152.29 | 3.88 |
2 | 2.0 | 0.2 | 0.10 | 0.3032 | 1.560 | 142.81 | 3.84 |
3 | 0.2 | 2.0 | 0.10 | 0.2290 | 1.855 | 222.7 | 4.03 |
4 | 2.0 | 2.0 | 0.10 | 0.1959 | 1.663 | 244.49 | 4.00 |
5 | 0.2 | 0.2 | 1.00 | 0.0883 | 2.146 | 302.90 | 3.91 |
6 | 2.0 | 0.2 | 1.00 | 0.1048 | 2.101 | 294.80 | 3.84 |
7 | 0.2 | 2.0 | 1.00 | 0.1061 | 2.339 | 335.62 | 3.93 |
8 | 2.0 | 2.0 | 1.00 | 0.1101 | 2.226 | 320.03 | 3.94 |
2.2.2. Continuous Gas-Fed Bioreactor Experiments
2.3. Analytical Equipment and Measurement Protocols
3. Results and Discussion
3.1. Bottle Batch Experiments
3.1.1. Main Effects Plot
3.1.2. Interaction Effects Plot
3.1.3. Effect of Individual Sources of Nitrogen on Growth and Product Formation
3.2. Continuous Gas-Fed Bioreactor Experiments
4. Conclusions
Supplementary Files
Supplementary File 1Acknowledgments
Author Contributions
Conflicts of Interest
References
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Abubackar, H.N.; Veiga, M.C.; Kennes, C. Ethanol and Acetic Acid Production from Carbon Monoxide in a Clostridium Strain in Batch and Continuous Gas-Fed Bioreactors. Int. J. Environ. Res. Public Health 2015, 12, 1029-1043. https://doi.org/10.3390/ijerph120101029
Abubackar HN, Veiga MC, Kennes C. Ethanol and Acetic Acid Production from Carbon Monoxide in a Clostridium Strain in Batch and Continuous Gas-Fed Bioreactors. International Journal of Environmental Research and Public Health. 2015; 12(1):1029-1043. https://doi.org/10.3390/ijerph120101029
Chicago/Turabian StyleAbubackar, Haris Nalakath, María C. Veiga, and Christian Kennes. 2015. "Ethanol and Acetic Acid Production from Carbon Monoxide in a Clostridium Strain in Batch and Continuous Gas-Fed Bioreactors" International Journal of Environmental Research and Public Health 12, no. 1: 1029-1043. https://doi.org/10.3390/ijerph120101029