LC/MS Analysis of Tetrodotoxin and Its Deoxy Analogs in the Marine Puffer Fish Fugu niphobles from the Southern Coast of Korea, and in the Brackishwater Puffer Fishes Tetraodon nigroviridis and Tetraodon biocellatus from Southeast Asia

Tetrodotoxin (TTX) and its deoxy analogs, 5-deoxyTTX, 11-deoxyTTX, 6,11-dideoxyTTX, and 5,6,11-trideoxyTTX, were quantified in the tissues of three female and three male specimens of the marine puffer fish, Fugu niphobles, from the southern coast of Korea, and in the whole body of the brackishwater puffer fishes, Tetraodon nigroviridis (12 specimens) and Tetrodon biocellatus (three specimens) from Southeast Asia using LC/MS in single ion mode (SIM). Identification of these four deoxy analogs in the ovarian tissue of F. niphobles were further confirmed by LC/MS/MS. TTX and 5,6,11-trideoxyTTX were detected in all three puffer fish species as the major TTX analogs, similar to Japanese Fugu pardalis. While 6,11-dideoxyTTX was also found to be a major analog in almost all tissues of Korean F. niphobles, this analog was minor in the two Tetraodon species and Japanese F. pardalis. Among the tissues of F. niphobles, the concentrations of TTXs were highest in the ovaries (female) and skin (female and male).

Herein we examined the contents of TTX and its deoxy analogs in the tissues of three female and three male specimens of the marine puffer fish F. niphobles collected at the southern coast of Korea, and in two brackishwater puffer fishes Tetraodon nigroviridis and Tetraodon biocellatus from Southeast Asia, purchased in a pet market in Japan [18], by LC/MS in HILIC mode.

Results and Discussion
TTXs were quantified by LC/MS in single ion monitoring (SIM) mode, because the ratios of signal to noise of the peaks of TTXs were higher than those obtained by LC/MS/MS in multiple reaction monitoring (MRM) mode, probably due to some specific reasons related to our MS spectrometer. Tetrodonic acid was not analyzed in this study. In Figure 2, LC/MS separation in SIM mode of (B) the ovary from female no. 3 specimen of Korean F. niphobles, (C) whole body of no. 5 specimen of T. nigroviridis, and (D) whole body of no. 2 specimen of T. biocellatus from Southeast Asia are illustrated as representatives. Based on the peak area on the mass chromatograms, the concentrations of TTXs in the ovary from female no. 3 F. niphobles are estimated at TTX (263 nmol/g), 4-epiTTX (26 nmol/g), 4,9-anhydroTTX (83 nmol/g), 5-deoxyTTX (22 nmol/g), 11-deoxyTTX (39 nmol/g), 6,11-dideoxyTTX (310 nmol/g), and 5,6,11-trideoxyTTX (603 nmol/g). Similarly, the toxin contents in each tissue of three female and three male specimens of F. niphobles, and those in whole body of twelve specimens of T. nigroviridis and three specimens of T. biocellatus are determined, and summarized in Table 1 and Table 2, respectively.  Table 1. Anatomical distribution of TTX and its analogs in F. niphobles (n = 3). Table 2. The contents of TTX and its analogs in the whole body extracts of T. nigroviridis (n = 12) and T. biocellatus(n = 3).

Species
Toxin content (nmol/g) TTX We previously reported characteristic fragment ions, including a major ion at m/z 162, from [M+H] + ions of TTX, 4-epiTTX, 4,9-anhydroTTX, 5-deoxyTTX, 11-deoxyTTX, and 5,6,11-trideoxyTTX by MS/MS (product ion) scan, and applied them to detection of TTXs by LC/MS/MS in MRM mode [8] as listed in Table 3. In the present study, we determined the major fragment ions by product ion scan from [M+H] + m/z 288 for 6,11-dideoxyTTX for the first time ( Figure 3). Distinctively from other TTX analogs [8], 6,11-dideoxyTTX produced the major fragment ion at m/z 224 instead at m/z162. Therefore, m/z 288-224 fragment was set up for detection of 6,11-dideoxyTTX by LC/MS/MS. Identification of TTX analogs by LC/MS in SIM mode was confirmed by LC/MS/MS in MRM mode, only for the ovary from female no. 3 Korean F. niphobles ( Figure 4B).

4-epiTTX 4,9-anhydro TTX
As shown in Table 1 and Table 2, TTX and 5,6,11-trideoxyTTX are commonly detected as the major toxins in all tissues of Korean F. niphobles and also in the two Tetraodon species, similar to Japanese Fugu pardalis as we reported previously [10]. Interestingly, 6,11-dideoxyTTX, which was previously discovered in the ovaries of Japanese F. pardalis as a minor analog [5], is detected as another major TTX analog in almost all tissues in Korean F. niphobles with exception of the skin from females. Also, the concentration of 6,11-dideoxyTTX is higher than that of TTX in the intestine and liver from female no. 3 F. niphobles. This analog is also detected in some specimens of T. nigroviridis and T. biocellatus as a minor analog. 5-DeoxyTTX and 11-deoxyTTX are contained in low concentrations in almost all tissues of Korean F. niphobles, but they are less than detection limit (<0.5 nmol/g) in the muscle and testicle. 11-DeoxyTTX is a minor analog also in the two Tetraodon species, while 5-deoxyTTX is slightly detected only in T. biocellatus and it is less than detection limit (<0.5 nmol/g) in T. nigroviridis. Except for 5-deoxyTTX in T. nigroviridis, these data suggest that these four deoxy analogs of TTX are commonly present in puffer fishes, although the ratios of them are specific to the species of puffer fishes or to the regions where the fishes were collected.   Among the tissues of Korean F. niphobles, the concentration of TTXs is highest in the ovary and skin. This is in agreement with the report by Ryu et al. [19] that skin was highly toxic (10-674 mouse unit/g = 7-465 nmol/g TTX) in Korean F. niphobles by mouse assay method (1 mouse unit is equivalent to 220 ng of TTX). Also, this is consistent with the data reported by Kono et al. [20] that dietarily administrated TTX to Japanese cultured juvenile F. niphobles was first accumulated in liver and then transferred to skin. According to Ikeda et al. [21], the high toxicity in the ovaries of Takifugu (=Fugu) poecilonotus might account for the TTXs transfer from liver, skin, intestine, and muscle into the ovaries during spawning season. Unlike Korean F. niphobles, Japanese F. pardalis contained the highest concentration of TTXs in ovary and liver, and medium level TTXs in skin [10]. Edible parts except intestine and ovary of F. niphobles, which contained fewer than 10 mouse units per gram, are used in a favorite fish soup or other food materials in Korea and cause TTX poisoning sporadically. More careful precautions need to be taken to remove toxic body parts before cooking. Concerning the toxicity of the puffer fishes genus Tetraodon, Shin-Jung et al. [22] examined the toxicity of 42 and 12 specimens of T. ocellatus and T. nigroviridis, respectively, collected in Taiwan by using mouse bioassay, and identified TTX and 4,9-anhydroTTX in both species by LC. The authors reported the highest toxicity score of T. nigroviridis to be 124 mouse unit/g (=85 nmol/g TTX), rather lower than those found in our present study (Table 2).

Puffer fishes specimens
Specimens of three female and three male each of F. niphobles (body weight 12-37 g) were collected on May 12 th , 2008 at Tongyeong bay, South Korea. The twelve specimens of T. nigroviridis (body weight 0.8-3.8 g, sex not identified) and three specimens of T. biocellatus (body weight 1.6-3.7g, sex not identified) collected from Southeast Asia were purchased in a pet market of Miyagi Prefecture, Japan. Samples were immediately kept on ice, transported to the laboratory and kept frozen below -20 °C for no longer than a month until use.

Preparation of sample solutions
Sample solutions were prepared as we reported previously [8,9] with minor modification. F. niphobles were dissected to obtain organs of ovary/testis, liver, intestine, dorsal skin and dorsal muscle, while T. nigroviridis and T. biocellatus were used as whole body. The toxins were extracted from a part (1 g) of homogenized tissues with 5 mL of 0.05 M acetic acid (v/v) by heating in boiling water for 5 min. The obtained suspensions were centrifuged at 4,000 rpm for 15 min at 4 °C, then the supernatants were filled up to 5 mL with 0.05 M acetic acid. An aliquot (2 mL) was applied to the reverse phase cartridge column (Sep-Pak C18, Waters) which was equilibrated with water after washing with MeOH before use. The first passing solution (1.5 mL) was discarded, and the following 0.5 mL was collected and defatted with CHCl 3 (0.5 mL). After removal of the remaining CHCl 3 under the stream of N 2 gas, the aqueous phase was lyophilized and filled up to 5 mL with water, and then loaded on an activated charcoal (0.5 mL), which was packed in a glass pipette. After washing the column with water (0.5 mL), the toxins were eluted with acetic acid/EtOH/H 2 O (1:50:49, 1.5 mL), and the volatile was evaporated from the eluate. The remaining residue was dissolved in 0.5 mL of 0.05 M acetic acid, and then ultrafiltered (Ultrafree-MC, 10,000 NMWL, Millipore, Bedford, MA) by centrifugation at 8,000 rpm for 15 min at 4 °C. This filtrate was used as the sample solution for LC/MS and LC/MS/MS analysis.

Preparation of semi-purified TTXs mixture as the standard for LC/MS
Semi-purified TTXs mixture was prepared from the ovary of Fugu poecilonotus, collected in Shimonoseki, Japan, by extraction of toxins with hot 0.05 M acetic acid, followed by partition with ethyl acetate and purification on a charcoal column [6]. TTX, 4-epiTTX, 4,9-anhydroTTX, 5-deoxyTTX, 11-deoxyTTX, 6,11-dideoxyTTX, and 5,6,11-trideoxyTTX in the obtained mixture were quantified using LC/MS in SIM mode on the basis of the standard curve for each analog drawn using highly purified analogs [8][9][10]. Because highly purified TTX analogs are very limited, we used this semi-purified TTXs mixture as the standard for LC/MS.

LC/MS, MS/MS, and LC/MS/MS
LC/MS was performed based on HILIC as we reported previously [9,10]. Briefly, LC system consisted of a Shimadzu LC-10AD pump (Japan) and a TSKgel Amide-80 column (2.0-i.d. x 150 mm, 5 μm, Toso, Tokyo, Japan). LC was performed using an aqueous solution containing 16 mM ammonium formate buffer (pH 5.5) and acetonitrile (3:7, v/v) as a mobile phase at a flow rate of 0.2 mL/min at 25 °C. LC/MS, LC/MS/MS and flow-injection MS/MS experiments were recorded on API2000 mass spectrometer (Applied Bio-systems MDS SCIEX, Foster City, CA) equipped with an ESI source in the positive-ion mode. ESI was evoked by a spray voltage of +5.5 kV and heated capillary temperature was maintained at 500 °C. Six ions at m/z 272, 288, 302, 304 and 320 corresponding to the [M+H] + ions of TTX analogs were detected in SIM mode, respectively. The dwell time was set at 200 ms per Da. The MS/MS measurements of 6,11-dideoxyTTX was based on collision induced dissociation (CID) occurring in the collision cell (Q2) of the triple quadrupole at a collision energy of 42 eV. Nitrogen was used as the target gas. 150 pmol of authentic 6,11-dideoxyTTX [5] (4 μl in 0.05M acetic acid) was introduced into the mass spectrometer by flow injection with methanol at a flow rate of 0.2 mL/min. The LC/MS/MS was performed under the same conditions as those used for the LC/MS and MS/MS experiments. The ions m/z 320-162 for TTX and 4-epiTTX, m/z 302-162 for 4,9-anhydroTTX, m/z 304-162 for 5-deoxyTTX and 11-deoxyTTX, m/z 288-224 for 6,11-dideoxyTTX, and m/z 272-162 for 5,6,11-tridexyTTX were detected in MRM mode [8]. The minimum TTX and its analogs to be detected were 0.5 nmol/g tissue in SIM mode.

Conclusions
In this study, four deoxy analogs of TTX were all detected in three species of puffer fishes tested with exception of 5-deoxyTTX in T. nigroviridis. We also found these deoxy analogs in ovary from Japanese Fugu poecilonotus when we prepared standard mixture of TTXs for LC/MS as described in the Experimental section. These results indicate that the deoxy analogs of TTX are common analogs in a range of puffer fishes. For the next work, we should investigate whether these deoxy analogs are chemically modified with each other in puffer fishes, or if they are provided from external origin, such as bacteria [14,15] and then accumulated in puffer fishes. The ability of puffer fishes to accumulate TTX was confirmed by Yamamori et al. [23] and Kono et al.[20] by the fact that cultured juvenile nontoxic Japanese Takifugu (Fugu) niphobles become toxic when TTX and its analogs were dietarily administered. Kono et al. [24] also reported that cultured juvenile F. niphobles administered TTX by intramuscular injection did not transform TTX to deoxy analogs or to 11-oxoTTX (which has CHO in the position of R 4 in Figure 1 instead of CH 2 OH of TTX). However, transformation from deoxy analogs or 11-oxoTTX to TTX has not been tested, and dietary administration might be important for such experiments. We will further examine whether these deoxy analogs are the products of transformation or metabolites in puffer fishes.