Anti-Inflammatory Halogenated Monoterpenes from the Red Alga Portieria hornemannii

The chemical investigation of a red alga Portieria hornemannii enabled the identification of three new halogenated monoterpenes (1–3) along with two previously identified metabolites (4 and 5). Their structures were determined by spectroscopic analysis and also by utilizing single-crystal diffraction analysis and quantum chemical calculation, as well as by comparison with literature data. Further corrections for dichloro and dibromo carbons using the sorted training set (STS) method were established in this study to significantly improve the accuracy in GIAO 13C NMR calculation of compounds 1–3. To discover the potential bioactive metabolites from P. hornemannii, the anti-inflammatory activities of all compounds were examined. Compounds 1 and 3–5 showed significant anti-inflammatory activity to inhibit the production of pro-inflammatory cytokines in the LPS-stimulated mature dendritic cells.


Introduction
Halogenated natural products were once dismissed as either artifacts of the isolation process or aberrations in nature [1].However, it is now recognized that organohalides are synthesized by various organisms, serving diverse roles and often in significant quantities.Red algae, which are widely distributed across the world's oceans, serve as abundant sources of proteins, fibers, vitamins, physiologically essential fatty acids, and macro-and trace elements.These valuable components can be utilized in food additives and healthcare products [2].The investigation of red algae constituents has been a subject of study for over four decades [3].Notably, marine algae from the genera Plocamium, Portieria, Laurencia, and Ochtodes have been identified as significant sources of halogenated monoterpenes, encompassing both acyclic and cyclic structural variations [4,5].Research by Naylor et al. suggests that ocimene acts as the common precursor for all halogenated monoterpenes found in Plocamium, while myrcene serves as the shared precursor in Portieria and Ochtodes, both of which belong to the family Rhizophyllidaceae [6,7].These compounds are recognized as defensive substances, protecting the algae against herbivores [6].Moreover, the metabolites derived from marine algae have demonstrated noteworthy potential in the fields of cosmeceuticals and medicine.They exhibit a range of beneficial properties, including skin whitening, anti-aging, anti-inflammatory, antioxidant, antimicrobial, and antitumor activities [7,8].
Halomon, a compound with the chemical name [6R-bromo-3S-bromomethyl)-7-methyl-2,3,7-trichloro-1-octene], was initially isolated from the red alga Portieria hornemannii (Lynbye) collected in the Philippines back in 1992 [9]. Remarkably, halomon exhibited significant differential cytotoxicity against cell lines derived from the brain, kidney, and colon, as determined by the National Cancer Institute's in vitro human tumor cell line screen [9,10].Based on its unique cytotoxicity profile, halomon was selected by the NCI for further investigation as a potential candidate for preclinical drug development [9,11].However, progress in utilizing halomon as an anticancer lead has been hindered by the lack of a reliable natural source and the absence of demonstrated in vivo effects [12].Efforts to re-isolate halomon from P. hornemannii collected from various locations in the Pacific Ocean, including Batan Island in the Philippines, have proven unsuccessful due to variations in terpene content across different sites and over time [13].Nonetheless, researchers have successfully achieved chemical syntheses of halomon and its analogues, albeit with challenges in achieving precise regio-and stereocontrol [14,15].Notably, Schlama et al. reported a highly efficient total synthesis of halomon, yielding it and a range of analogues with an overall yield of 13% [16].
A comprehensive literature review revealed that a total of 26 halogenated monoterpenes have been isolated from the red alga Portieria hornemannii in previous studies [4,9,[17][18][19][20][21].However, over the past decade, no reports have emerged regarding the bioactive constituents of species from the genus Portieria.This knowledge gap, coupled with the inspiration derived from the potential of halomon, motivated us to embark on the exploration and discovery of new bioactive halogenated monoterpenes from the red alga, P. hornemannii.In this study, we present the isolation and characterization of three new halogenated monoterpenes (1-3) (Figure 1), along with two known compounds (4 and 5) [22,23], obtained from specimens collected off the coast of Penghu Islands.Additionally, we conducted an anti-inflammatory assay for all isolated compounds in order to identify potential leads for drug development.monoterpenes, encompassing both acyclic and cyclic structural variations [4,5].Research by Naylor et al. suggests that ocimene acts as the common precursor for all halogenated monoterpenes found in Plocamium, while myrcene serves as the shared precursor in Portieria and Ochtodes, both of which belong to the family Rhizophyllidaceae [6,7].These compounds are recognized as defensive substances, protecting the algae against herbivores [6].Moreover, the metabolites derived from marine algae have demonstrated noteworthy potential in the fields of cosmeceuticals and medicine.They exhibit a range of beneficial properties, including skin whitening, anti-aging, anti-inflammatory, antioxidant, antimicrobial, and antitumor activities [7,8].
Halomon, a compound with the chemical name [6R-bromo-3S-bromomethyl)-7-methyl-2,3,7-trichloro-1-octene], was initially isolated from the red alga Portieria hornemannii (Lynbye) collected in the Philippines back in 1992 [9]. Remarkably, halomon exhibited significant differential cytotoxicity against cell lines derived from the brain, kidney, and colon, as determined by the National Cancer Institute's in vitro human tumor cell line screen [9,10].Based on its unique cytotoxicity profile, halomon was selected by the NCI for further investigation as a potential candidate for preclinical drug development [9,11].However, progress in utilizing halomon as an anticancer lead has been hindered by the lack of a reliable natural source and the absence of demonstrated in vivo effects [12].Efforts to re-isolate halomon from P. hornemannii collected from various locations in the Pacific Ocean, including Batan Island in the Philippines, have proven unsuccessful due to variations in terpene content across different sites and over time [13].Nonetheless, researchers have successfully achieved chemical syntheses of halomon and its analogues, albeit with challenges in achieving precise regio-and stereocontrol [14,15].Notably, Schlama et al. reported a highly efficient total synthesis of halomon, yielding it and a range of analogues with an overall yield of 13% [16].
A comprehensive literature review revealed that a total of 26 halogenated monoterpenes have been isolated from the red alga Portieria hornemannii in previous studies [4,9,[17][18][19][20][21].However, over the past decade, no reports have emerged regarding the bioactive constituents of species from the genus Portieria.This knowledge gap, coupled with the inspiration derived from the potential of halomon, motivated us to embark on the exploration and discovery of new bioactive halogenated monoterpenes from the red alga, P. hornemannii.In this study, we present the isolation and characterization of three new halogenated monoterpenes (1-3) (Figure 1), along with two known compounds (4 and 5) [22,23], obtained from specimens collected off the coast of Penghu Islands.Additionally, we conducted an anti-inflammatory assay for all isolated compounds in order to identify potential leads for drug development.
(3R,4S,5E,7S)-4-Bromo-3,7,8,8-tetrachloro-3,7-dimethyl-octa-1,5-diene (1) was obtained in the form of needle-shaped crystals.Analyzing its HREIMS spectrum, a molecular formula of C10H13BrCl4 was deduced, with four isotope peaks observed at m/z 351, 353, 355, and 357, exhibiting approximate intensities of 4:10:8:3, respectively [24] (see Figures S1 and S2 in Supplementary Materials).The 13   [7], a dichloromethyl group (δH 5.79, s; δC 78.4,CH), and two methyl groups attached to halogenated quaternary carbons (δH 1.81, s; 1.89, s; δC 25.6, CH3; 24.9, CH3) were observed.Based on these findings, the structure of compound 1 was suggested to be a halogenated acyclic monoterpene.The determination of the planar structure of compound 1 was achieved through a comprehensive analysis of COSY and HMBC spectra.The COSY correlations unveiled two separate spin systems, namely H2-1/H-2 and H-4/H-5/H-6.Moreover, the HMBC correlations observed from H3-9 to C-2, C-3, and C-4, along with correlations from H3-10 to C-6, C-7, and C-8, effectively connected the two spin systems and subsequently confirmed the planar structure of compound 1, as depicted in Figure 2    The relative configurations at C-3 and C-4 were determined by a comparison of 13 C NMR data of the C-3 methyl shift with those of related analogues in literature [7], in which the methyl shift of δ C 25-26 was suggested for 3,4-erythro compound and δ C 28 for 3,4-threo isomer.Consequently, a 3,4-erythro configuration was assigned for compound 1 (δ C 25.6, C-9).The relative configuration at C-7 was determined by the application of the sorted training set (STS) method allowed for theoretical NMR calculations.This protocol was utilized to enhance the precision and dependability of structure assignment, particularly in cases involving carbons bearing heavy atoms (Cl, S, Br, I, etc.) [25].The calculation data (uncorrected data, Table 2) could not show a conclusive result, with P rel probabilities of 43.55% and 56.45% for 3R*,4S*,7S*-1 and 3R*,4S*,7R*-1, respectively.This was ascribed to the lack of corrections for dichloro carbons in the STS method.Thus, 18 dichloro model compounds from the literature were selected and their NMR data were computed following the STS protocol to obtain a correction formula (I corr = 0.8613I cald + 29.499,I calcd : calculated shielding tensor).Using the correction formula for dichloro carbon (C-8) in 1, the mean absolute error (MAE) and root mean square (rms) values of 3R*,4S*,7S*-1 are as low as 1.07 and 1.33, respectively, which are much better than the uncorrected data.Furthermore, 3R*,4S*,7S*-1 was suggested to be the correct structure with the P rel probability of 77.33%, which is much higher than that of 3R*,4S*,7R*-1 (22.67%).To further confirm the structure and determine the absolute configuration of 1, a single-crystal X-ray diffraction analysis was performed.The X-ray crystallography data from 1 with a Flack parameter of 0.001(9) demonstrated its structure and absolute configuration to be 3R,4S,7S (Figure 3). a The calculated chemical shifts were obtained following the STS protocol.b The chemical shifts were obtained following the STS protocol except that the chemical shifts of C-8 (dichloromethine) were obtained from a corrected shielding tensor using the formula (I corr = 0.8613I cald + 29.499), and subsequently converted to chemical shift using the linear equation (δ = −1.0266Icorr + 199.81) from the reported STS protocol.
Mar. Drugs 2023, 21, 493 4 of 12 The relative configurations at C-3 and C-4 were determined by a comparison of 13 C NMR data of the C-3 methyl shift with those of related analogues in literature [7], in which the methyl shift of δC 25-26 was suggested for 3,4-erythro compound and δC 28 for 3,4-threo isomer.Consequently, a 3,4-erythro configuration was assigned for compound 1 (δC 25.6, C-9).The relative configuration at C-7 was determined by the application of the sorted training set (STS) method allowed for theoretical NMR calculations.This protocol was utilized to enhance the precision and dependability of structure assignment, particularly in cases involving carbons bearing heavy atoms (Cl, S, Br, I, etc.) [25].The calculation data (uncorrected data, Table 2) could not show a conclusive result, with Prel probabilities of 43.55% and 56.45% for 3R*,4S*,7S*-1 and 3R*,4S*,7R*-1, respectively.This was ascribed to the lack of corrections for dichloro carbons in the STS method.Thus, 18 dichloro model compounds from the literature were selected and their NMR data were computed following the STS protocol to obtain a correction formula (Icorr = 0.8613Icald + 29.499, Icalcd: calculated shielding tensor).Using the correction formula for dichloro carbon (C-8) in 1, the mean absolute error (MAE) and root mean square (rms) values of 3R*,4S*,7S*-1 are as low as 1.07 and 1.33, respectively, which are much better than the uncorrected data.Furthermore, 3R*,4S*,7S*-1 was suggested to be the correct structure with the Prel probability of 77.33%, which is much higher than that of 3R*,4S*,7R*-1 (22.67%).To further confirm the structure and determine the absolute configuration of 1, a single-crystal X-ray diffraction analysis was performed.The X-ray crystallography data from 1 with a Flack parameter of 0.001(9) demonstrated its structure and absolute configuration to be 3R,4S,7S (Figure 3).(3R*,4S*,5E)-3,4,7,8,8-Pentachloro-3,7-dimethyl-octa-1,5-diene (2) was isolated as a colorless oil.The high-resolution chemical ionization mass spectrometry (HRCIMS) analysis of its spectrum revealed the molecular formula to be C 10 H 13 Cl 5 (see Figures S10 and S11 in Supplementary Materials).The 13 C NMR and DEPT spectra of compound 2 revealed the presence of 10 carbon signals, encompassing two methyl groups, one methylene group, five methine groups, and two quaternary carbons, as outlined in Table 1.The findings, combined with a thorough comparison of NMR data between 1 and 2, indicated that 2 is a polychlorinated monoterpene closely related to 1 (as shown in Table 1).The most significant disparity was observed for the brominated methine in 1 (δ C 60.4, C-4), where it was substituted by a chlorine atom in 2 (δ C 67.9, C-4) [7].The HMBC correlation between H 3 -9 (δ H 1.75, s) and C-2 (δ C 139.5), C-3 (δ C 71.7), as well as C-4 (δ C 67.9), further supported the aforementioned deduction (refer to Figure 2 and Figures S12-S18 in Supplementary Materials).By examining the carbon resonance of the C-3 methyl group of 2 (δ C 24.9, C-9), the configuration of 3,4-erythro was established [7].Similar to compound 1, the relative configuration at C-7 of 2 was determined using the STS method with the established dichloro correction.The 3R*,4S*,7S*-configuration was suggested for 2 due to its P rel probability of 71.24% (Table 3).Moreover, the NOE correlations of 2 showed close similarity to those of 1, conforming the same configurations of both compounds (Figure 2). a The calculated chemical shifts were obtained following the STS protocol.b The chemical shifts were obtained following the STS protocol except that the chemical shifts of C-8 (dichloromethine) were obtained from a corrected shielding tensor using the formula (I corr = 0.8613I cald + 29.499), and subsequently converted to chemical shift using the linear equation (δ = −1.0266Icorr + 199.81) from the reported STS protocol.
The carbon attached to a bromine or chlorine atom could deshield the chemical shift and the effect for the chlorine atom is generally larger.Similarly, the presence of a remaining sp 2 methine at δ C 117.2 (C-8) suggested that chlorine is attached at this position.This conclusion is drawn by comparing the data with the existing literature, which indicates that vinyl bromide is typically observed at a chemical shift of approximately δ C 108-110 ppm [7], while vinyl chloride is typically observed at 118-124 ppm [7,26].A 15.2 Hz of 1 H-1 H coupling constant gave evidence for E-geometry of the 5,6-double bond in 3 (see Table S1 in Supplementary Materials).Based on the result reported by Mynderse and Faulkner as well as the synthetic analogues [22,26], the geometry of the 7,8-double bond was found to be associated with the chemical shift of H-6 and it can be concluded that, in the 5,7-diene system with C-8 halogen trans to H-6, the proton chemical shift of H-6 is found at approximately δ H 6.2-6.3 (in CDCl 3 ).However, in the cis analogue, the shift is found at approximately δ H 6.5-6.8 (in CDCl 3 ).The H-6 proton NMR shift of compound 3, found at δ H 6.39 in CDCl 3 (Table 1), indicated that its 7,8-double bond has Z geometry.Moreover, the proton shift of the H-6 in compound 3, measured in acetone-d 6 , was found to be at δ H 6.55.This value is in close proximity to the known compounds 4 (δ H 6.52) and 5 (δ H 6.53) as shown in Table S1.These findings suggest the presence of a 7Z double bond in compound 3. To provide further confirmation, the application of the STS method for theoretical NMR calculation was performed on 3. The result (Table 4) showed high deviation at dibromo methine carbon C-10 (∆ = 19.17ppm for 7Z and 23.29 ppm for 7E, ∆ = δ calcd − δ exptl ), which can be explained by the lack of corrections for dibromo carbons in the STS strategy.Thus, we selected 10 dibromo model compounds from literature and computed their NMR data following the STS protocol to obtain a correction formula (I corr = 0.6209I calcd + 81.575,I calcd : calculated shielding tensor) (Figure 4).Using the correction formula for dibromo carbon resulted in a significant improvement in accuracy for C-10 (Table 4).Furthermore, replacement of C-10 data by the corrected shielding tensor would show good results (P rel probability, 100%), which confirmed the 7Z configuration for 3. a The calculated chemical shifts were obtained following the STS protocol.b The chemical shifts were obtained following the STS protocol except that the chemical shifts of C-10 (dibromomethine) were obtained from a corrected shielding tensor using the formula (I corr = 0.6209I cald + 81.575), and subsequently converted to chemical shift using the linear equation (δ = −1.0266Icorr + 199.81) from the reported STS protocol.To date, more than 100 halogenated monoterpenes have been discovered from different organisms [27].Some halogenated monoterpenes have been demonstrated to show cytotoxic [9,17,18], antimalarial [28], and insecticidal activities [29][30][31].In the present study, we isolated four new halogenated monoterpenoids, and two reported isolates.To discover any potential drug leads, all of the metabolites were examined with the anti-inflammatory assay.Compounds 1, 4, and 5 were found to significantly reduce the production of TNF-α in the LPS-stimulated dendritic cells with IC50 values of 2.5 ± 0.4, 6.2 ± 1.1, and 10.6 ± 1.3, respectively (Table 5), which is more potent than the positive control, quercetin (IC50 = 23.1 ± 5.2).To date, more than 100 halogenated monoterpenes have been discovered from different organisms [27].Some halogenated monoterpenes have been demonstrated to show cytotoxic [9,17,18], antimalarial [28], and insecticidal activities [29][30][31].In the present study, we isolated four new halogenated monoterpenoids, and two reported isolates.To discover any potential drug leads, all of the metabolites were examined with the anti-inflammatory assay.Compounds 1, 4, and 5 were found to significantly reduce the production of TNF-α in the LPSstimulated dendritic cells with IC 50 values of 2.5 ± 0.4, 6.2 ± 1.1, and 10.6 ± 1.3, respectively (Table 5), which is more potent than the positive control, quercetin (IC 50 = 23.1 ± 5.2).The comparison of anti-inflammatory activity between 1 and 2 indicated that the bromine atom at C-4 seems to be essential for the biological activity.Additionally, in the case of 3-5, compound 3 with two bromine atoms at C-10 did not show any activity in the dose test.However, two chlorine atoms attached at C-10 seem to be responsible for the biological activity.Based on the above evidence, an undiscovered halogenated monoterpene 6 (Figure 5) that might show potential anti-inflammatory activity is waiting for discovery in the future.The comparison of anti-inflammatory activity between 1 and 2 indicated that the bromine atom at C-4 seems to be essential for the biological activity.Additionally, in the case of 3-5, compound 3 with two bromine atoms at C-10 did not show any activity in the dose test.However, two chlorine atoms attached at C-10 seem to be responsible for the biological activity.Based on the above evidence, an undiscovered halogenated monoterpene 6 (Figure 5) that might show potential anti-inflammatory activity is waiting for discovery in the future.

General Experimental Procedures
Measurements of optical rotations were conducted for compounds 1-5 utilizing a JASCO P-1020 polarimeter (JASCO Corporation, Tokyo, Japan), and their NMR spectra

General Experimental Procedures
Measurements of optical rotations were conducted for compounds 1-5 utilizing a JASCO P-1020 polarimeter (JASCO Corporation, Tokyo, Japan), and their NMR spectra were recorded on Varian 400 MR FT-NMR and Varian Unity INOVA500 FT-NMR (Varian Inc., Palo Alto, CA, USA) instruments at 400 MHz and 500 MHz for 1 H and 100 MHz and 125 MHz for 13 C in CDCl 3 and acetone-d 6 at 25 • C. Electron impact mass spectrometry (EIMS) and HREIMS spectra were afforded on the magnetic sector mass spectrometry (JEOL, JMS-700, Tokyo, Japan).A normal-phase column packed with silica gel (Merck, 230-400 mesh) was utilized in the experiment, while thin-layer chromatography (TLC) with pre-coated silica gel plates (Merck, Kieselgel 60 F-254, 0.2 mm, Merck, Darmstadt, Germany) was employed for analyzing each isolated fraction.Additionally, high-performance liquid chromatography was conducted using a Supelco C18 column (250 × 21.2 mm, 5 µm; Supelco, Bellefonte, PA, USA) and a Hitachi L-2455 HPLC apparatus.

Plant Material
The red alga P. hornemannii (wet weight: 1.53 kg) was collected by hand using SCUBA from the sea area near the Pescadores Islands in 2012.Until the commencement of the experiment, the material sample had been kept at the Department of Marine Biotechnology and Resources, National Sun Yat-sen University.The red alga was identified by Dr. Wei-Lung Wang.

Quantum Chemical Calculations
Two possible candidates for each compound were subjected to conformational searches on GMMX add-on of GaussView 6 (Semichem Inc., Shawnee Mission, KS, USA).This was followed by preliminary optimizations of conformers within a 5 kcal/mol energy window at the B3LYP/6-31G(d) level of theory using Gaussian 16 software (Gaussian, Inc., Wallingford, CT, USA) [32].After removing duplicates, further optimizations and frequency calculations were computed at the B3LYP-D3(BJ)/TZVP level, employing the IEFPCM solvent mode with CHCl 3 as the solvent.Structures with imaginary frequencies were excluded from subsequent calculations.Theorical shielding tensors were calculated at the IEFPCM (CHCl 3 )/ωB97xD/6-31G(d) level of theory.The data were weighted according to Boltzmann population using Gibbs free energy and subjected to STS analysis [25].All calculations were performed with the "g09defdaults" keyword in Gaussian 16 to ensure consistent results, as suggested in the literature [25].A total of 18 and 10 model compounds were selected from the literatures for dichloro-and dibromo-carbon shift corrections, respectively (Supplementary Materials: Figures S30 and S31).The calculated shielding tensors for the model compounds were obtained following the same protocol as described above.The experimental chemical shifts were retroconverted into pseudo shielding tensors (I retro ) by the equation: I retro = (199.81− δ exptl )/1.0266 [25] and then regressed with calculated shielding tensors (I calcd ) to obtain the correction linear equation as shown in Figure 4.

Anti-Inflammatory Assay
In this study, the viability of D.C. cells was evaluated at a concentration of 100 µg/mL using a cell-counting-kit assay (CCK-8) obtained from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan), which measured absorbance at a wavelength of 450 nm, following the methodology outlined by Chung et al. [33].The expression of the pro-inflammatory cytokine, TNF-α, in the culture supernatant was screened using the dendritic cells ELISA kit, as described in a previous study [34].The cytotoxicity of quercetin against DCs was measured in this study as a positive control, with IC 50's of 78.8 ± 7.3 µM.Additionally, quercetin exhibited inhibitory effects on the reduction of TNF-α expression in LPS-induced DCs, with IC 50's of 23.1 ± 5.2.

Conclusions
The present investigation on the red alga P. hornemannii led to the characterization of three new halogenated monoterpenes and two reported analogues, of which the structures were elucidated based on the analysis of their MS and NMR spectroscopic data.We also determined the absolute configuration of 1 through its X-ray crystallography data.Compound 3, which contains a rare 1-chloro-3,3-dibromoprop-1-en-2-yl moiety, has been found for the first time in P. hornemannii.In the anti-inflammatory assay, compounds 1, 4, and 5 were demonstrated to significantly reduce the TNF-α in the LPS-stimulated dendritic cells.Evaluation of the result of biological assays concluded that the structural motifs with C-4 bromo and C-10 dichloro substituents in acyclic monoterpenes are important for anti-inflammatory activity.Our research has once again confirmed that the red alga P. hornemannii is a good source of bioactive halogenated monoterpenes.

a
The calculated chemical shifts were obtained following the STS protocol.b The chemical shifts were obtained following the STS protocol except that the chemical shifts of C-10 (dibromomethine) were obtained from a corrected shielding tensor using the formula (Icorr = 0.6209Icald + 81.575), and subsequently converted to chemical shift using the linear equation (δ = −1.0266Icorr+ 199.81) from the reported STS protocol.

Figure 4 .
Figure 4. Linear relationship between Iretro (y axis, experimental chemical shifts retroconverted into pseudo experimental shielding tensors) and Icalcd (x axis, shielding tensors from computational calculation) for corrections of (a) dichloro and (b) dibromo carbons following STS method.

Figure 4 .
Figure 4. Linear relationship between I retro (y axis, experimental chemical shifts retroconverted into pseudo experimental shielding tensors) and I calcd (x axis, shielding tensors from computational calculation) for corrections of (a) dichloro and (b) dibromo carbons following STS method.

Table 1 .
(see Figures S1 and S2 in Supplementary Materials).The 13 C NMR and DEPT spectra of compound 1 displayed 10 carbon signals, encompassing two methyl groups, one methylene group, five methine groups, and two quaternary carbons, as indicated in Table 1.Additionally, distinctive signals corresponding to a mono-substituted double bond (δ H 5.27, d, 1 H (400 MHz) and 13 C NMR (100 MHz) spectroscopic data for 1

Table 4 .
GIAO13C NMR calculations for 7Z and 7E isomers of 3 following the STS method.

Table 5 .
Anti-inflammatory result of compounds 1

Table 5 .
Anti-inflammatory result of compounds 1