Bio-Active Products from Mangrove Ecosystems

Mangrove communities represent the coastal habitats located in intertidal zones or brackish waters of tropical and subtropical coastal areas in over 118 countries [...].

The second review by Sulaiman et al. [3] provides a comprehensive review of antibacterial, antifungal, and antiviral natural products from the mangrove plants in Asia and the Pacific reported from 1968 to 2022.Among the 286 plant species, 119 exhibited antimicrobial effects, and a total of 114 antimicrobial natural products have been identified including 12 with MIC values below 1 µg/mL.A vast array of antimicrobial secondary metabolites that could be further examined for development of anti-infectives to mitigate infectious diseases such as the White Spot Syndrome Virus infection in aquaculture is described.In parallel, the use and promotion of mangrove plants in aquacultures are beneficial as the rise in the global population which requires a huge supply shrimps, prawns, crabs, and fish globally necessitates the preservation of mangroves.
Small molecules different mechanisms of fibrinolysis action are desired for new antithrombotics and thrombolytics.Hang et al. [4] list a series of bioactive staplabin congeners which not only possess fibrinolytic activity but also exhibit various effects, such as anti-inflammatory, neuroprotective, and anti-cancer properties.The authors focused on the diverse biological activities of SMTP-7 (compound 1, also known as FGFC1), an isoindolone alkaloid from the marine fungi Starchbotrys longispora FG216 and Stachybotrys microspora IFO 30018, that possesses diverse bioactivities such as thrombolytic, anti-inflammatory, and anti-oxidative properties and selective anti-cancer activity.These properties make SMTP-7 an attractive option for the development of drugs for the treatment of various diseases, including cerebral infarction, stroke, ischemia/reperfusion damage, acute kidney injury, non-small cell lung cancer, and especially for cerebral infarction.The recent progress in structure-function relationships, preparation methods, identification of diverse biological activities and mechanism of SMTP-7 and its congeners is summarized, thereby illustrating its high therapeutic potential.
Wang et al. [7] first systematically evaluated the diversity of cultivable fungi associated with the medicinal mangrove Acanthus ilicifolius collected from the South China Sea.A total of 102 fungal strains were isolated from A. ilicifolius and 84 independent culturable isolates were identified using a combination of morphological characteristics and internal transcribed spacer (ITS) sequence analyses, of which 37 strains were selected for phylogenetic analysis.The identified fungi belonged to 22 genera within seven taxonomic orders of one phyla, of which four genera Verticillium, Neocosmospora, Valsa, and Pyrenochaeta were first isolated from mangroves.Thirty-one strains of fungi displayed strong cytotoxicity to different human cancer cell lines: A-549, HeLa, HepG, and Jurkat.Integrating a cytotoxic activity-guided strategy and fingerprint analysis, a well-known natural Golgi-disruptor and Arf-GEF inhibitor, brefeldin A, was quickly isolated from the active strains.
Feng et al. [8] reported three previously undescribed cytochalasins, namely phomoparagins A-C, together with five previously reported analogs from the mangrovederived endophytic fungus Phomopsis asparagi DHS-48.Notably, phomoparagin A possessed an unprecedented 5/6/5/8/5-fused pentacyclic skeleton.These were tested for their inhibitory activity against concanavalin A (ConA)/lipopolysaccharide (LPS)-induced spleen lymphocyte proliferation and the calcineurin (CN) enzyme.Phomoparagin B, phomopchalasins A and B, as well as cytochalasin H exhibited robust inhibitory activities with a relatively low toxicity.Moreover, phomoparagin B was shown to inhibit ConA-stimulated activation of NFAT1 dephosphorylation and block NFAT1 translocation in vitro, subsequently inhibiting the transcription of interleukin-2 (IL-2), whereas it directly inhibited calcineurin and did not require a matchmaker protein, such as the clinical immunosuppressants CsA and FK506.
Epigenetic manipulation was described as an effective method to stimulate gene clusters which are 'silent', 'orphan', and 'cryptic' for enhancing secondary metabolite expression without altering genes or causing the hereditable manipulation of microorganisms.Feng et al. [9] conducted an epigenetic manipulation of the aforementioned Phomopsis asparagi DHS-48 with the histone deacetylase (HDAC) inhibitor sodium butyrate and the DNA methyltransferase (DNMT) inhibitor 5-azacytidine (5-Aza).Based on the colony growth, dry biomass, HPLC, and 1 H NMR analyses, the fungal chemical profile was significantly different compared to the control, and an optional modifier (50 µM sodium butyrate) was selected for the follow up fermentation.Two undescribed compounds, named phaseolorin J and phomoparagin D, along with three previously reported chromones and previously described cytochalasins, were isolated from the culture treated with sodium butyrate.Their structures, including absolute configurations, were elucidated using a combination of detailed HRESIMS, NMR, and ECD analyses and 13 C NMR calculations.Phaseolorin J and phaseolorin J2 were found to be moderate immunosuppressants, inhibiting the proliferation of ConA (concanavalin A)-induced T and LPS (lipopolysaccharide)-induced B murine spleen lymphocytes.Additionally, phomoparagin D exhibited significant in vitro cytotoxicity against the human cancer cell lines Hela and HepG2.
Red yeast rice (Monascus-fermented rice, also called anka or koji) has been used as a natural food coloring additive and in traditional Chinese medicine since ancient times due to its ability to ease digestion and antiseptic effects.A novel strain Monascus purpureus wmd2424 was isolated from the mangrove in Chiayi Wetland.It was identified by colony culture morphology, microstructural characteristics, and partial sequence analysis of the β-tubulin gene fragments by Wu et al. [11].The authors described the isolation of five previously unreported compounds, named monascuspurins A-E from the EtOAc extract of wmd2424 cultured in RGY medium.Of these, monascuspurins C-E exhibited mild antifungal activity against the tested Aspergillus niger, Penicillium italicum, Candida albicans, and Saccharomyces cerevisiae.
We would like to thank all the authors for their contribution to this Special Issue.The articles on the topic presented in this Special Issue revealed that mangrove ecosystems is especially attractive due to its abundant microbial communities, such as diverse strains of endophytic fungi from mangrove vegetation and sediments that produce various secondary metabolites with unusual skeletons.These compounds usually possess highly selective and specific biological activities with unique mechanisms of action, offering a promising prospect of mangrove ecosystems to serve as an unlimited reservoir for lead discovery and drug development.Biogenetic manipulation to stimulate silent or cryptic biosynthetic genes offers an effective strategy for mining untapped natural products from microorganisms.Nevertheless, the biosynthesis of most of the natural products derived from mangrove-associated microorganisms and their corresponding biosynthetic gene clusters in the mentioned microbial strains remain elusive.

Conflicts of Interest:
The authors declare no conflict of interest.

Funding:
Co-financed by grants from the National Natural Science Foundation of China (No. 82160675/81973229), the Key Research Program of Hainan Province (ZDYF2021SHFZ108), and the Key Science and Technology Project of Hainan Province (ZDKJ202018).