Induction of New Aromatic Polyketides from the Marine Actinobacterium Streptomyces griseorubiginosus through an OSMAC Approach

Using the OSMAC (One Strain Many Compounds) approach, the actinobacterium Streptomyces griseorubiginosus, derived from an unidentified cnidarian collected from a reef near Pointe de Bellevue in Réunion Island (France), was subjected to cultivation under diverse conditions. This endeavour yielded the isolation of a repertoire of 23 secondary metabolites (1–23), wherein five compounds were unprecedented as natural products (19–23). Specifically, compounds 19 and 20 showcased novel anthrone backbones, while compound 23 displayed a distinctive tetralone structure. Additionally, compounds 21 and 22 presented an unusual naphtho [2,3-c]furan-4(9H)-one chromophore. Interestingly, the detection of all these novel compounds (19–23) was exclusively achieved when the bacterium was cultured in FA-1 liquid medium supplemented with the epigenetic modifier γ-butyrolactone. The elucidation of the structural features of the newfound compounds was accomplished through a combination of HRESIMS, 1D and 2D NMR spectroscopy, as well as QM-NMR (Quantum Mechanical—Nuclear Magnetic Resonance) methods and by comparison with existing literature. Moreover, the determination of the relative configuration of compound 23 was facilitated by employing the mix-J-DP4 computational approach.


Introduction
Natural products of microbial origin, especially those from actinobacteria, have been proven to be successful drug leads [1][2][3].However, standard culture conditions often fail to reveal their full biosynthetic potential and leads to re-isolation of already known metabolites.Strategies to unlock silent biosynthetic gene clusters that are not expressed using conventional fermentation methods include the so-called OSMAC (One Strain Many Compounds) approach [4][5][6][7][8][9].The OSMAC approach has been shown as a simple and powerful tool to promote the production of different secondary metabolites, potentially bioactive, by altering simple culture parameters, such as variations in medium composition or the addition of epigenetic modifiers (chemical elicitors).The fungus Sphaeropsidales sp. was known to produce cladospirone bisepoxide, a spirobisnaphthalene compound.However, Bode et al. [10], reported that culturing the same strain in altered cultivation conditions, different media, and cultivation vessels led to the isolation of fourteen additional compounds, including eight new cladospirones B-I.Notably, the highest number of metabolites was produced when this strain was subjected to solid-phase cultivation in P-flasks with wet oat grains as a single substrate.
As part of our ongoing research on marine microorganisms, we investigated the actinobacterium Streptomyces griseorubiginosus, derived from an unidentified cnidarian collected at a reef close to Pointe de Bellevue in Réunion Island (France), following an OSMAC approach.Streptomyces griseorubiginosus has been previously reported to produce several antibiotic secondary metabolites, such as the angucyclinone-type rubiginones A1, B1, C1, and C2 [11]; the 15-membered cyclic peptides biphenomycins A-C [12]; the glycosilated anthracyclines cinerubins A and B [13]; and the alkaloid reductiomycin [14].
In this study, the most noteworthy effects with regard to an alteration of the bacterial metabolite pattern were detected following the addition of γ-butyrolactone to the FA-1 liquid medium, compared to the standard culture lacking this chemical elicitor.The investigation of the bacterial extract obtained from fermentation of Streptomyces griseorubiginosus under these conditions led to the isolation of five new aromatic polyketides (19)(20)(21)(22)(23), in addition to several known compounds, all of which were not detected when the fungus was grown without this activator.Herein, we report the isolation and the structure elucidation of the new metabolites discovered.

Isolation of Secondary Metabolites from S. griseorubiginosus through OSMAC
The fermentation of the microorganism Streptomyces griseorubiginosus was carried out under standard conditions (FA-1 medium), and five natural products (1-5) were isolated and identified; all of them were anthraquinone and anthrone derivatives (Figure 1).
of metabolites was produced when this strain was subjected to solid-phase cultivation in P-flasks with wet oat grains as a single substrate.
As part of our ongoing research on marine microorganisms, we investigated the actinobacterium Streptomyces griseorubiginosus, derived from an unidentified cnidarian collected at a reef close to Pointe de Bellevue in Réunion Island (France), following an OSMAC approach.Streptomyces griseorubiginosus has been previously reported to produce several antibiotic secondary metabolites, such as the angucyclinone-type rubiginones A1, B1, C1, and C2 [11]; the 15-membered cyclic peptides biphenomycins A-C [12]; the glycosilated anthracyclines cinerubins A and B [13]; and the alkaloid reductiomycin [14].
In this study, the most noteworthy effects with regard to an alteration of the bacterial metabolite pattern were detected following the addition of γ-butyrolactone to the FA-1 liquid medium, compared to the standard culture lacking this chemical elicitor.The investigation of the bacterial extract obtained from fermentation of Streptomyces griseorubiginosus under these conditions led to the isolation of five new aromatic polyketides (19)(20)(21)(22)(23), in addition to several known compounds, all of which were not detected when the fungus was grown without this activator.Herein, we report the isolation and the structure elucidation of the new metabolites discovered.
Streptomyces griseorubiginosus was then cultured on SAF medium, yielding five further secondary metabolites (6-10), which were not detected from the standard condition culture.
Streptomyces griseorubiginosus was then cultured on SAF medium, yielding five further secondary metabolites (6-10), which were not detected from the standard condition culture.

Structure Elucidation of Novel Compounds
Compound 19 was isolated and purified to obtain a yellowish amorphous solid.Analysis by HRESIMS and NMR data determined its molecular formula to be C16H14O5 (ten degrees of unsaturation).1D NMR spectra were very similar to those of previously isolated anthrone-derived molecules.In comparison with (+)-Oryzanthrone B (4), the presence of an oxygenated methylene (δH 4.66, 4.92, δC 65.9) was observed instead of the methyl (δH 2.68, δC 24.3) attached to the aromatic ring.The gHMBC correlations observed from H2-11 to C-1, C-2 and C-10a confirmed the replacement of the methyl group by the oxygenated methylene (Figure 5).Further 2D NMR analysis confirmed that the rest of the molecule was identical to 4, determining the structure of 19 as shown in Compound 20 was a yellowish amorphous solid with the molecular formula C16H14O5 determined by HRESIMS and NMR data (10 degrees of unsaturation).An initial study of the 1D NMR spectra of 20 (Table 1) revealed that this compound was a new anthrone derivative.Comparison of the 1 H and 13 C NMR spectra with those of (+)-Oryzanthrone B Known compounds 16-18 were identified as γ-butyrolactam (16) [26], GTRI-02 (17) [27,28], and 6-dehydroxy-GTRI-02 (18) [28] after extensive spectroscopic analysis and comparison with data reported in the literature.

Structure Elucidation of Novel Compounds
Compound 19 was isolated and purified to obtain a yellowish amorphous solid.Analysis by HRESIMS and NMR data determined its molecular formula to be C 16 H 14 O 5 (ten degrees of unsaturation).1D NMR spectra were very similar to those of previously isolated anthrone-derived molecules.In comparison with (+)-Oryzanthrone B (4), the presence of an oxygenated methylene (δ H 4.66, 4.92, δ C 65.9) was observed instead of the methyl (δ H 2.68, δ C 24.3) attached to the aromatic ring.The gHMBC correlations observed from H 2 -11 to C-1, C-2 and C-10a confirmed the replacement of the methyl group by the oxygenated methylene (Figure 5).Further 2D NMR analysis confirmed that the rest of the molecule was identical to 4, determining the structure of 19 as shown in Figure 4.The third novel natural product produced by Streptomyces griseorubiginosus using γbutyrolactone as an epigenetic modifier on FA-1 medium was a yellowish amorphous solid (21) with the molecular formula C16H14O5, established by HRESIMS and NMR data (10 degrees of unsaturation).Compound 20 was a yellowish amorphous solid with the molecular formula C 16 H 14 O 5 determined by HRESIMS and NMR data (10 degrees of unsaturation).An initial study of the 1D NMR spectra of 20 (Table 1) revealed that this compound was a new anthrone derivative.Comparison of the 1 H and 13 C NMR spectra with those of (+)-Oryzanthrone B (4) showed the similarity between these two molecules, the only difference being the presence of a hydroxy group attached to C-7.The molecular mass of 20, 16 amu higher than that of 4, supported this suggestion. 1 H-NMR and gCOSY experiments suggested the presence of an anthrone scaffold, with two tetrasubstituted aromatic rings with two protons in meta each (J 2-4 = 2.5 Hz; J 6-8 = 2.4 Hz).These data together with the observed correlations in gHMBC experiments from H-2 and H-4 to C-10a and from H-6 to C-5, C-8 and C-9a confirmed the structure of compound 20 as shown in Figures 4 and 5.The third novel natural product produced by Streptomyces griseorubiginosus using γ-butyrolactone as an epigenetic modifier on FA-1 medium was a yellowish amorphous solid (21) with the molecular formula C 16 H 14 O 5 , established by HRESIMS and NMR data (10 degrees of unsaturation).
The 1 H NMR spectrum of compound 21 was very similar to that of previously elucidated anthrone-derived molecules; however, more detailed analysis of 13 C and 2D NMR experiments suggested some differences in the chemical scaffold of 21.The gHMBC correlations (see Table 2) and chemical shifts of H-6, H-7, H-8, and H 3 -13 confirmed that the naphtho moiety was identical to that of compounds 2, 4, and 19.On the other side, gHMBC correlations from H 3 -12 to C-3 (δ C 161.6) and C-3a (δ C 117.5) and from H 3 -11 to C-1 (δ C 145.6) and C-10 (δ C 190.4), shown in Figure 6, in conjunction with molecular weight information, suggested that compound 21 was 1-acetyl-5,9-dihydroxy-3,9-dimethylnaphtho[2,3-c]furan-4(9H)-one.The structure of 21 was subsequently confirmed by comparison with the data described in the literature for other polyketides with the naphtho[2,3-c]furan-4(9H)-one backbone [29,30].The 1 H NMR spectrum of compound 21 was very similar to that of previously elucidated anthrone-derived molecules; however, more detailed analysis of 13 C and 2D NMR experiments suggested some differences in the chemical scaffold of 21.The gHMBC correlations (see Table 2) and chemical shifts of H-6, H-7, H-8, and H3-13 confirmed that the naphtho moiety was identical to that of compounds 2, 4, and 19.On the other side, gHMBC correlations from H3-12 to C-3 (δC 161.6) and C-3a (δC 117.5) and from H3-11 to C-1 (δC 145.6) and C-10 (δC 190.4), shown in Figure 6, in conjunction with molecular weight information, suggested that compound 21 was 1-acetyl-5,9-dihydroxy-3,9-dimethylnaphtho[2,3-c]furan-4(9H)-one.The structure of 21 was subsequently confirmed by comparison with the data described in the literature for other polyketides with the naphtho[2,3-c]furan-4(9H)-one backbone [29,30].To determine the absolute configuration of 21, TDDFT/ECD (time-dependent density functional theory/electronic circular dichroism) calculations were performed at the PCM/B3LYP/6-31G(d) level of theory using structures previously optimized at the same level.It has to be noted that due to the rigidity of the molecule only one suitable structure was found in a molecular mechanics conformational search within a threshold of 21kJ/mol.As observed in Figure 7, the experimental spectrum showed negative Cotton effects at ~210 nm and ~305 nm and positive Cotton effects at ~250 nm and ~350 nm.The To determine the absolute configuration of 21, TDDFT/ECD (time-dependent density functional theory/electronic circular dichroism) calculations were performed at the PCM/B3LYP/6-31G(d) level of theory using structures previously optimized at the same level.It has to be noted that due to the rigidity of the molecule only one suitable structure was found in a molecular mechanics conformational search within a threshold of 21 kJ/mol.As observed in Figure 7, the experimental spectrum showed negative Cotton effects at ~210 nm and ~305 nm and positive Cotton effects at ~250 nm and ~350 nm.The calculated spectrum for the 9S enantiomer clearly matched better with the experimental data, allowing us to assign the absolute configuration of 21.Similar results were obtained using a different level of theory (ωB97XD) (see Supporting Information).To determine the absolute configuration of 21, TDDFT/ECD (time-dependent density functional theory/electronic circular dichroism) calculations were performed at the PCM/B3LYP/6-31G(d) level of theory using structures previously optimized at the same level.It has to be noted that due to the rigidity of the molecule only one suitable structure was found in a molecular mechanics conformational search within a threshold of 21kJ/mol.As observed in Figure 7, the experimental spectrum showed negative Cotton effects at ~210 nm and ~305 nm and positive Cotton effects at ~250 nm and ~350 nm.The calculated spectrum for the 9S enantiomer clearly matched better with the experimental data, allowing us to assign the absolute configuration of 21.Similar results were obtained using a different level of theory (ωB97XD) (see Supporting Information).Compound 22 was isolated as a whitish amorphous solid with the molecular formula C16H14O4 determined based on HRESIMS and NMR data (10 degrees of unsaturation).3) were very similar to those of GTRI-02 (17).The presence of a new oxygenated quadruplet methine group (δ H 5.31, δ C 68.0) coupled to H 3 -10 methyl group (J = 6.7 Hz) in 23, instead of the ketone group at C-9 in GTRI-02, suggested the reduction of the ketone to alcohol, which was also evident from the molecular formula of 23 (C 13 H 16 O 4 ).Further 2D NMR analysis confirmed the structure of 23 as shown in Figures 4 and 8. thine group (δH 5.31, δC 68.0) coupled to H3-10 methyl group (J = 6.7 H the ketone group at C-9 in GTRI-02, suggested the reduction of the which was also evident from the molecular formula of 23 (C13H16O4 analysis confirmed the structure of 23 as shown in Figures 4 and 8.  Therefore, two remote chiral centres are present in 23, making it very difficult to determine their relative configurations.The use of quantum mechanical calculations to unravel the information encoded in NMR chemical shifts, also known as the QM-NMR method, is currently one of the most popular strategies for this purpose.There are several methods for this task; however, those based on Bayes' theorem, such as the original DP4 probability or the more elaborated DP4+ and J-DP4 methods, stand out from the rest [31][32][33][34].Previous experience with these approaches [35][36][37][38][39][40][41][42] led us to select mix-J-DP4 [43] as the most appropriate method in this case, as it yields high confidence results at modest computational costs by incorporating proton-proton NMR coupling constants.Thereby, a conformational search was achieved using the mixed torsional/low-mode protocol in gas phase by means of MMFF force field; later, the conformers were reoptimized with an AMBER and MM3 force field.The energy cut-off of 12 kJ/mol and geometric criteria of MAD 0.5 Å were used to eliminate duplicate structures; after that, NMR calculations were performed at the J-DP4 recommended level (B3LYP/6-31G**) and 3 J HH used only the Fermi Contact term contribution.Finally, a comparison of calculated and experimental NMR data was undertaken as described for the J-DP4 protocol, obtaining an overall probability of >99% for isomer 3R*,9R*.Moreover, the R 2 s obtained with each force field are: AMBER 0.999 and 0.987, MM3 0.999 and 0.993, MMFF 0.999 and 0.987, for carbon and proton, respectively, and the 13 C CMAE value for AMBER and MM3 is 1.5 ppm and for MMFF 1.8 ppm.The 1 H CMAE value obtained for all force fields is 0.1 ppm.
A conformational search analysis shows that there exist two major conformations (Figure 9): one conformer where H3 is in an axial position with a contribution of 57-65% (depending on the force field used), and the other conformer which represents 35-43% of the total population that has the same proton in an equatorial position.This hints that there exists a conformational equilibrium between both conformations, which agrees with the experimental results obtained in 3 J H3 (8.1 and 4.0 Hz).
Therefore, two remote chiral centres are present in 23, making it very difficult to determine their relative configurations.The use of quantum mechanical calculations to unravel the information encoded in NMR chemical shifts, also known as the QM-NMR method, is currently one of the most popular strategies for this purpose.There are several methods for this task; however, those based on Bayes' theorem, such as the original DP4 probability or the more elaborated DP4+ and J-DP4 methods, stand out from the rest [31][32][33][34].Previous experience with these approaches [35][36][37][38][39][40][41][42] led us to select mix-J-DP4 [43] as the most appropriate method in this case, as it yields high confidence results at modest computational costs by incorporating proton-proton NMR coupling constants.Thereby, a conformational search was achieved using the mixed torsional/low-mode protocol in gas phase by means of MMFF force field; later, the conformers were reoptimized with an AMBER and MM3 force field.The energy cut-off of 12 kJ/mol and geometric criteria of MAD 0.5 Å were used to eliminate duplicate structures; after that, NMR calculations were performed at the J-DP4 recommended level (B3LYP/6-31G**) and 3 JHH used only the Fermi Contact term contribution.Finally, a comparison of calculated and experimental NMR data was undertaken as described for the J-DP4 protocol, obtaining an overall probability of >99% for isomer 3R*,9R*.Moreover, the R 2 s obtained with each force field are: AMBER 0.999 and 0.987, MM3 0.999 and 0.993, MMFF 0.999 and 0.987, for carbon and proton, respectively, and the 13 C CMAE value for AMBER and MM3 is 1.5 ppm and for MMFF 1.8 ppm.The 1 H CMAE value obtained for all force fields is 0.1 ppm.
A conformational search analysis shows that there exist two major conformations (Figure 9): one conformer where H3 is in an axial position with a contribution of 57-65% (depending on the force field used), and the other conformer which represents 35-43% of the total population that has the same proton in an equatorial position.This hints that there exists a conformational equilibrium between both conformations, which agrees with the experimental results obtained in 3 JH3 (8.1 and 4.0 Hz).All isolated compounds were subjected to antibacterial and cytotoxic (against different tumour cell lines) assays, but none of them showed any remarkable bioactivity.Attempts to obtain more available material of those compounds are currently ongoing, in order to screen them in some relevant crop-protection assays.

General Experimental Procedures
Optical rotations were measured on a JASCO P-2000 polarimeter using MeOH as solvent.CD spectra were recorded on a JASCO J-810 spectropolarimeter.IR data were recorded using a BRUKER VECTOR 22 spectrophotometer. 1 H NMR and 13 C NMR data were obtained on a Varian "Mercury 400" spectrometer at 400 and 100 MHz, respectively.Chemical shifts are reported in ppm relative to solvent (CDCl 3 : δ = 7.26/77.16ppm; [D 4 ] MeOH: δ= 3.31/49.0ppm).HPLC-MS experiments were carried out with an Agilent UHPLC 1290 Infinity II coupled to an Agilent TOF 6230.HPLC analysis were performed with a Zorbax Eclipse plus C18 column, using a linear gradient from 20% to 100% methanol in 8 min, a post time of 2 min, and a DAD analysis 200-600 nm range signal obtained at 220 nm.Mass spectrometer conditions in ESI-Positive were 100 V Fragmentor, mass range (m/z) = 100-3200.The analysis were performed with Masshunter suite software (Agilent Technologies) version B.08.00.An Agilent 1200 series liquid chromatograph with a photodiode array and evaporative light-scattering detectors was used for HPLC analysis and recording UV spectra and t R values, performed with a Zorbax Eclipse XDB-C18 column (4.6 × 50 mm, 1.8 µm) maintained at 20 • C with a mobile phase flow rate of 0.5 mL/min, using the following linear gradient of MeOH/H 2 O (+0.04% TFA): Min 0-15: 15-100% MeOH; min 15-20: 100% MeOH; min 20-30: 100-15% MeOH, post-run 5 min, and a DAD analysis 200-600 nm range signal obtained at 220 nm.Semipreparative HPLC purifications were conducted on an Agilent 1100 series (Kinetex 5 µm EVO C18 10 column, 100 × 21.2 mm).

Isolation of the Strain
Strain AA-AR-H-B002 was isolated from an unidentified cnidarian collected at a reef close to Pointe de Bellevue in Réunion Island (France).The strain has been deposited in the Colección Española de Cultivos Tipo, Valencia, Spain, with the accession number CECT 30830.

Identification of the Strain
The strain was identified as Streptomyces griseorubiginosus by partial sequencing of its 16S RNA, showing a 99.78% homology in a 913-nucleotide-length sequence.The DNA of the microorganism was obtained using a Qiagen Dneasy kit following the manufacturer's instructions.The 16S rDNA gene was amplified using the universal primers 27f (AGAGTTTGATCMTGGCTCAG) and 1492r (TACGGYTACCTTGTTACGACTT).The PCR program used was as follows: 94 • C for 1 min, then 30 cycles of 98 • C for 10 s, 50 • C for 2 min, 172 • C for 10 s, and finally one step of 72 • C for 7.5 min.The amplified gen was purified using the Qiagen QiaQuick kit following the manufacturer's directions.The purified amplicons were sent to an external service for sequencing.All the different steps were monitored using 1.2% agarose electrophoresis in half-strength TB buffer.

Fermentations
All the liquid fermentations were carried out in flasks, incubated at 28 • C and with an agitation at 240 rpm.All the fermentations started from a fresh, confluent culture of the actinomycete on 172 solid medium incubated for one week at 28 • C. The inoculum was developed in two stages.The first stage was carried out in Erlenmeyer flasks of 50 mL capacity and containing 10 mL of medium MIM.These flasks were seeded with 3 plugs taken from the lawn of the confluent solid culture.The flasks were incubated as described above for 48 h.The second stage was carried out in Erlenmeyer flasks of 250 mL capacity filled with 40 mL of MIM medium and seeded with 4 mL of the first stage.The flasks were incubated as described for 48 h.The production phase was performed using Erlenmeyer flasks of 2 L capacity and filled with 250 mL of the appropriate medium.These production flasks were seeded with 12 mL of the second-stage inoculum and incubated as described previously for 96 or 120 h depending on the experiment.The epigenetic factor γ-butyrolactone was added to the corresponding medium (FA-1) at a concentration of 50 µM.
All the solid fermentations were carried out in 2 L capacity Erlenmeyer flasks, containing 250 mL of solid medium SymPv-21.The seeded flasks were incubated at 28 ºC in aerobic conditions for 144 h.

Extraction and Isolation
Fermentation broth (8 L) of Streptomyces griseorubiginosus under standard conditions (FA-1 liquid medium) was stirred with Amberlite XAD1180N™ resin (10% v/v) for 1 h.Then, culture broth was filtered through Celite ® , and the mycelial cake and the resin were extracted with 4 L of a mixture of EtOAc/MeOH (3:1).The resultant suspension was filtered and partitioned between EtOAc and water.The organic layer was taken to dryness, and the crude extract (3.9 g) was fractionated by VFC (vacuum-flash chromatography) on silica gel, eluting with a stepwise gradient of hexane/EtOAc/MeOH to give 12 fractions (1F1-1F12).Fractions 1F11-12 were further fractionated by normal-phase liquid chromatography using a stepwise gradient of CH 2 Cl 2 and MeOH to yield 12 subfractions (2F1-2F12).Subfractions 2F7-10 and 2F5 were purified by reversed-phase liquid chromatography using a stepwise gradient of MeOH/H 2 O to give 1 (3.7 mg, t R 14.2 min) and 2 (13.2 mg, t R 12.3 min), respectively.Compounds 3 (12.5 mg, t R 16.1 min) and 5 (27.4 mg, t R 15.8 min) were isolated from fraction 1F5 by normal-phase liquid chromatography with a gradient of hexane/EtOAc followed by a further step of normal-phase liquid chromatography with a gradient of hexane/ether, while compound 4 (74.5 mg, t R 13.9 min) was obtained from fractions 1F6-7, following a similar procedure, with the difference that the last step of purification was carried out by normal-phase liquid chromatography with a gradient of CH 2 Cl 2 /MeOH.
The same extraction procedure was followed for all liquid medium fermentations.The chromatographic work up of the OSMAC extracts followed the same procedure as described for the standard-condition culture.Silica gel vacuum-flash chromatography using a stepwise gradient of hexane/EtOAc/MeOH was used for separation of the crude extracts obtained from SAF medium (4.55 g) and FA-1 medium with the addition of the chemical elicitor γ-butyrolactone (4.94 g).A total of twelve fractions (SA1F1-SA1F12) were obtained from the SAF culture extract.Fractions SA1F9-10 were further fractionated by normal-phase liquid chromatography using a stepwise gradient of CH 2 Cl 2 /MeOH to yield 10 subfractions (SA2F1-SA2F10).Subfraction SA2F5 was purified by reversed-phase liquid chromatography using a stepwise gradient of MeOH/H 2 O to give 6 (8.4 mg, t R 9.0 min), while subfractions SA2F7 and SA2F8 were also purified by MeOH/H 2 O reversed-phase liquid chromatography, yielding 7 (15.1 mg, t R 5.8 min), 8 (5.6 mg, t R 7.3 min), 9 (4.6 mg, t R 9.7 min), and 10 (3 mg, t R 10.9 min).Fraction B1F11, obtained by VFC from FA-1 culture after the addition of γ-butyrolactone extract, was fractionated by normal-phase liquid chromatography using a stepwise gradient of CH 2 Cl 2 /MeOH to yield 12 subfractions (B2F1-B2F12).Compound 16 (13.9mg, t R 1.2 min) was isolated from subfractions B2F5-7 after purification by reversed-phase liquid chromatography using a stepwise gradient of MeOH/H 2 O. Fractions B1F9-10 were also fractionated following the same procedure described for compound 16 to give 17 (46.9mg, t R 10.9 min), 18 ( (20), respectively, after purification by normal-phase liquid chromatography using a stepwise gradient of hexane/EtOAc, followed by semi-preparative HPLC purification with a gradient of MeOH/H 2 O as an eluting system.
In the case of the solid-state fermentation (SymPV-21 medium), the contents of the flasks were collected and homogenized with the aid of an electric mixer, followed by extraction with 100% v/v of a mixture of EtOAc/MeOH (3:1).Twelve fractions were obtained from the SymPV-21 solid culture extract (4.26 g) after VFC (Sy1F1-Sy1F12).Compounds 11 (41.1 mg, t R 8.6 min), 12 (56 mg, t R 8.0 min), and 13 (7.8 mg, t R 7.7 min) were isolated from Sy1F10 after purification by normal-phase liquid chromatography using a stepwise gradient of CH 2 Cl 2 /MeOH, followed by a further step of normal-phase liquid chromatography with a gradient of CH 2 Cl 2 /acetone.Finally, fraction Sy1F11-12 was also fractionated by normal-phase liquid chromatography, using a gradient of CH 2 Cl 2 /MeOH as mobile phase, followed by reversed-phase liquid chromatography using a stepwise gradient of MeOH/H 2 O to afford 14 (27.5 mg, t R 5.0 min) and 15 (8.4 mg, t R 9.7 min).

Figure 5 .
Figure 5. Key COSY and HMBC correlations of compounds 19 and 20.Based on the opposite sign of the optical rotation of compound 19 ([α] 20 D = −133.9• ) compared to that published for (+)-Oryzanthrone B ([α] 25 D = +10.7 • ), we tentatively assign an absolute configuration R to carbon 5 in this compound.Compound 20 was a yellowish amorphous solid with the molecular formula C 16 H 14 O 5 determined by HRESIMS and NMR data (10 degrees of unsaturation).An initial study of the 1D NMR spectra of 20 (Table1) revealed that this compound was a new anthrone derivative.Comparison of the 1 H and 13 C NMR spectra with those of (+)-Oryzanthrone B (4) showed the similarity between these two molecules, the only difference being the presence of a hydroxy group attached to C-7.The molecular mass of 20, 16 amu higher than that of 4, supported this suggestion.1 H-NMR and gCOSY experiments suggested the presence of an anthrone scaffold, with two tetrasubstituted aromatic rings with two protons in meta each (J 2-4 = 2.5 Hz; J 6-8 = 2.4 Hz).These data together with the observed correlations in gHMBC experiments from H-2 and H-4 to C-10a and from H-6 to C-5, C-8 and C-9a confirmed the structure of compound 20 as shown in Figures4 and 5.

Figure 7 .
Figure 7. Experimental and calculated ECD spectra for compound 21.

1 HFigure 7 .
Figure 7. Experimental and calculated ECD spectra for compound 21.Compound 22 was isolated as a whitish amorphous solid with the molecular formula C 16 H 14 O 4 determined based on HRESIMS and NMR data (10 degrees of unsaturation). 1 H NMR spectrum of compound 22 suggested a very similar structure to that of 21, the only difference being a quadruplet methine group (δ H 4.58, δ C 32.2) coupled to H 3 -13 methyl group (J = 7.1 Hz) in 22, instead of the quaternary carbon (δ C 67.7) at C-9 in 21.This suggested the loss of the hydroxy group attached to C-9 in 21, which was also evident from the molecular mass of 22 being 16 amu less than that of 21 (C 16 H 14 O 5 ), establishing compound 22 as 1-acetyl-5-hydroxy-3,9-dimethylnaphtho[2,3-c]furan-4(9H)-one (Figure 4).Compound 23 (yellowish amorphous solid) has the molecular formula C 13 H 16 O 4 (six degrees of unsaturation) based on HRESIMS and NMR data.NMR data (Table3) were very similar to those of GTRI-02 (17).The presence of a new oxygenated quadruplet methine group (δ H 5.31, δ C 68.0) coupled to H 3 -10 methyl group (J = 6.7 Hz) in 23, instead of the ketone group at C-9 in GTRI-02, suggested the reduction of the ketone to alcohol, which was also evident from the molecular formula of 23 (C 13 H 16 O 4 ).Further 2D NMR analysis confirmed the structure of 23 as shown in Figures4 and 8.

Table 1 .
NMR data of compounds 19 and 20.

Table 2 .
NMR data of compounds 21 and 22 in CDCl 3 .