Unusual Secondary Metabolites from the Mangrove Ecosystems: Structures, Bioactivities, Chemical, and Bio-Syntheses

Mangrove ecosystems are widely distributed in the intertidal zone of tropical and subtropical estuaries or coasts, containing abundant biological communities, for example, mangrove plants and diverse groups of microorganisms, featuring various bioactive secondary metabolites. We surveyed the literature from 2010 to 2022, resulting in a collection of 134 secondary metabolites, and classified them into two major families in terms of the biological sources and 15 subfamilies according to the chemical structures. To highlight the structural diversity and bioactivities of the mangrove ecosystem-associated secondary metabolites, we presented the chemical structures, bioactivities, biosynthesis, and chemical syntheses.


Introduction
Identifying lead compounds is one of the biggest challenges in drug discovery. Natural products (NPs) and their intricate molecular frameworks have a long tradition as valuable starting points for drug development (for example, artemisinins, taxol, camptothecin, and penicillins). To date, NPs remain a significant source of new compounds, providing a wide range of structural diversities with multiple privileged scaffolds for drug discovery either directly, semi-synthetically, or as a source of inspiration [1][2][3][4][5]. However, discovering new bioactive NPs is generally time-consuming and laborious. Only a few new NP drug pharmacophores have been found over the past 20 years, representing critical issues for NPs-driven lead discovery campaigns.
Mangrove forests are complex ecosystems widely distributed in tropical and subtropical estuaries or coastal intertidal zones. These forests contain diverse biological communities, including mangrove plants and numerous groups of microorganisms. The environment of the mangrove system harbors unique traits, for instance, high salinity, low oxygen, tidal gradients, high temperature, and excessively intense light, resulting in an active ecosystem with various microorganisms [1][2][3][4][5]. Mangrove-associated microorganisms have been demonstrated to be a reliable source of bioactive metabolites and have likewise drawn the attention of NP researchers [6][7][8][9][10][11][12]. A large number of structurally unusual and bioactive NPs have been discovered from the mangrove-associated microorganisms, including fungal and bacterial endophytes isolated from the mangrove plants' leaves, branches, and roots [13,14]. In addition, Mangrove sediments-derived microbes, a rich reservoir of NP diversity, could be utilized to explore new drugs [15].
The bioactive NPs solely from the true mangrove and semi-mangrove floras worldwide have been summarized in several review papers in 2010 [16,17]. However, the investigation of the mangrove ecosystem is mainly focused on the mangrove-associated microorganism but less on the mangrove flora in recent years. Three comprehensive reviews have recently focused on the NPs from mangrove-associated fungi and mangrove sediments-derived microbes [6,14,15]. However, to our knowledge, no review has been published on the secondary metabolites with unusual skeletons from the mangrove ecosystem. They might merit the attention of chemists and biologists and could be a source of fresh pharmacophores with biological activity for the creation of drugs based on natural products.
In this review, we focus on the mangrove ecosystem-associated NPs featuring new carbon scaffolds, unique ring systems, or unusual functional moieties covering from 2010 to 2022. The structures, biological activities, biosynthesis, and total chemical syntheses of exampled unique compounds were included.
The references were searched using the following keywords as the subject search: natural products/secondary metabolites, mangrove, via Web of Science, Chemical Abstracts, and PubMed databases covering from 2010 to 2022, resulting in a collection of 134 unusual secondary metabolites. We classified them into two major families in terms of biological sources.

Limonoids
Limonoids are natural tetranortriterpenoids containing a four-ring structure with a 17β-furyl ring mainly distributed in the Meliaceae and Rutaceae families [18]. In the mangrove flora, they are especially abundant and structurally diversified in the genus Xylocarpus moluccensis and X. grantum (family Meliaceae). Up to 2021, approximately 2700 limonoids have been identified. Moreover, owing to their widespread distribution and substantial content in Meliaceae plants, and active biosynthetic pathways, more than 1600, including 30 types of unique rearrangement skeletons, have been isolated and identified in the last 10 years [19]. Among them, nearly 233 new limonoids with 14 kinds of novel skeletons were isolated from mangrove Xylocarpus.

products.
In this review, we focus on the mangrove ecosystem-associated carbon scaffolds, unique ring systems, or unusual functional moieties to 2022. The structures, biological activities, biosynthesis, and total ch exampled unique compounds were included.
The references were searched using the following keywords as natural products/secondary metabolites, mangrove, via Web of Scie stracts, and PubMed databases covering from 2010 to 2022, resulting i unusual secondary metabolites. We classified them into two major biological sources.

Limonoids
Limonoids are natural tetranortriterpenoids containing a four-ri 17β-furyl ring mainly distributed in the Meliaceae and Rutaceae famil grove flora, they are especially abundant and structurally diversified carpus moluccensis and X. grantum (family Meliaceae). Up to 2021, app monoids have been identified. Moreover, owing to their widespread d stantial content in Meliaceae plants, and active biosynthetic pathwa including 30 types of unique rearrangement skeletons, have been iso in the last 10 years [19]. Among them, nearly 233 new limonoids wit skeletons were isolated from mangrove Xylocarpus.
An unusual tetranortriterpenoid, xylomexicanins E (23) (Figure 7), which is the first example of limonoid with azaspiro skeleton between B (pyrrolidine) and C rings, was isolated from the seeds of the Chinese mangrove, X. granatum [23]. The plausible biosynthetic routes are proposed, as shown in Scheme 2, starting from the limonoid prexylogranatopyridine. Two unprecedented limonoids, thaixylomolins B and C (19 and 20) (Figure 5), coisolates with 1 [21], are limonoids containing a unique pentasubstituted pyridine scaffold that might be generated by aromatization into a pyridine ring from a phargmalin-class limonoid. (Scheme 1) Thaixylomolin B (19) exhibited inhibitory activity against nitric oxide production in lipopolysaccharide and IFN-γ-induced RAW264.7 murine macrophages with an IC 50 value of 84.3 µM.
An unusual tetranortriterpenoid, xylomexicanins E (23) (Figure 7), which is the first example of limonoid with azaspiro skeleton between B (pyrrolidine) and C rings, was isolated from the seeds of the Chinese mangrove, X. granatum [23]. The plausible biosynthetic routes are proposed, as shown in Scheme 2, starting from the limonoid prexylogran- Two pyridine-containing limonoids, xylogranatopyridines A (21) and B (22) ( Figure  6), were isolated from the twigs and leaves of X. granatum, collected from the seashore of Dongzhai, Hainan Province [32]. Compared to 21, xylogranatopyridine B (22) possesses an unprecedented rearranged A-ring. Prexylogranatopyridine, a co-occurrence of limonoid with an unusual C-8-C-30 linkage, could be the common biosynthetic precursor of 21 and 22 (Scheme 2). Xylogranatopyridine A (21) exhibited significant inhibitory activity against protein tyrosine phosphatase 1B (PTP1B) with an IC50 value of 22.9 μM. An unusual tetranortriterpenoid, xylomexicanins E (23) (Figure 7), which is the first example of limonoid with azaspiro skeleton between B (pyrrolidine) and C rings, was isolated from the seeds of the Chinese mangrove, X. granatum [23]. The plausible biosyn- Further investigation of the seeds from the X. granatum led to the isolation of two new tetranortriterpenoids, xylomexicanins I and J (24 and 25) [33] (Figure 7). Notably, 24 represents an unprecedented limonoid with a bridged skeleton between the B-and Crings, contrasting with analogues possessing bridged A-and B-rings (25). Wu et al. proposed that 24 was obtained from the same natural precursor as 22 after an enolate addition to the allylic alcohol moiety between C-3 and C-11 (Scheme 2).
Three new limonoids, entitled xylomolones A-C (26-28, respectively, Figure 8) were discovered from the seeds of the Thai mangrove X. moluccensis, as well as a vital biosynthetic precursor, xylomolone D (a new C11-terpenic acid methyl ester) [34]. Compared to 26, compound 27 is the first 9,10-seco limonoid with a 3,4-dihydro-2H-pyran motif and possesses the reversed alignment of ring A. For the biosynthesis of xylomolone C (28), a five-membered A-ring could be built through a benzylic acid-like rearrangement, forming an unusual 3-oxabicyclo[3.2.1]octan-2,7-dione motif; the C-2 is excluded from the A-ring in the rearrangement process. Wu et al. proposed a novel convergent strategy for limonoid biosynthesis (Scheme 3).

Diterpenoids
Two new ent-isopimarane-type diterpenoids agallochaexcoerins D and E (29 and 30) [35] (Figure 9), possessing an unusual seven-membered lactone moiety, were isolated from the wood of mangrove Excoecaria agallocha. Notably, 29 is the first report of ent-3,4secoisopimaratriene diterpenoid with a rare seven-membered ring. Further investigation of the seeds from the X. granatum led to the isolation of two new tetranortriterpenoids, xylomexicanins I and J (24 and 25) [33] (Figure 7). Notably, 24 represents an unprecedented limonoid with a bridged skeleton between the B-and C-rings, contrasting with analogues possessing bridged A-and B-rings (25). Wu et al. proposed that 24 was obtained from the same natural precursor as 22 after an enolate addition to the allylic alcohol moiety between C-3 and C-11 (Scheme 2).
Three new limonoids, entitled xylomolones A-C (26-28, respectively, Figure 8) were discovered from the seeds of the Thai mangrove X. moluccensis, as well as a vital biosynthetic precursor, xylomolone D (a new C11-terpenic acid methyl ester) [34]. Compared to 26, compound 27 is the first 9,10-seco limonoid with a 3,4-dihydro-2H-pyran motif and possesses the reversed alignment of ring A. For the biosynthesis of xylomolone C (28), a five-membered A-ring could be built through a benzylic acid-like rearrangement, forming an unusual 3-oxabicyclo[3.2.1]octan-2,7-dione motif; the C-2 is excluded from the A-ring in the rearrangement process. Wu et al. proposed a novel convergent strategy for limonoid biosynthesis (Scheme 3). Further investigation of the seeds from the X. granatum led to the isolation of two new tetranortriterpenoids, xylomexicanins I and J (24 and 25) [33] (Figure 7). Notably, 24 represents an unprecedented limonoid with a bridged skeleton between the B-and C rings, contrasting with analogues possessing bridged A-and B-rings (25). Wu et al. pro posed that 24 was obtained from the same natural precursor as 22 after an enolate addition to the allylic alcohol moiety between C-3 and C-11 (Scheme 2).
Three new limonoids, entitled xylomolones A-C (26-28, respectively, Figure 8) were discovered from the seeds of the Thai mangrove X. moluccensis, as well as a vital biosyn thetic precursor, xylomolone D (a new C11-terpenic acid methyl ester) [34]. Compared to 26, compound 27 is the first 9,10-seco limonoid with a 3,4-dihydro-2H-pyran motif and possesses the reversed alignment of ring A. For the biosynthesis of xylomolone C (28), a five-membered A-ring could be built through a benzylic acid-like rearrangement, forming an unusual 3-oxabicyclo[3.2.1]octan-2,7-dione motif; the C-2 is excluded from the A-ring in the rearrangement process. Wu et al. proposed a novel convergent strategy for limonoid biosynthesis (Scheme 3).

Diterpenoids
Two new ent-isopimarane-type diterpenoids agallochaexcoerins D and E (29 and 30 [35] (Figure 9), possessing an unusual seven-membered lactone moiety, were isolated from the wood of mangrove Excoecaria agallocha. Notably, 29 is the first report of ent-3,4 secoisopimaratriene diterpenoid with a rare seven-membered ring. Further investigation of the seeds from the X. granatum led to the isolation of two new tetranortriterpenoids, xylomexicanins I and J (24 and 25) [33] (Figure 7). Notably, 24 represents an unprecedented limonoid with a bridged skeleton between the B-and C rings, contrasting with analogues possessing bridged A-and B-rings (25). Wu et al. pro posed that 24 was obtained from the same natural precursor as 22 after an enolate addition to the allylic alcohol moiety between C-3 and C-11 (Scheme 2).
Three new limonoids, entitled xylomolones A-C (26-28, respectively, Figure 8) were discovered from the seeds of the Thai mangrove X. moluccensis, as well as a vital biosyn thetic precursor, xylomolone D (a new C11-terpenic acid methyl ester) [34]. Compared to 26, compound 27 is the first 9,10-seco limonoid with a 3,4-dihydro-2H-pyran motif and possesses the reversed alignment of ring A. For the biosynthesis of xylomolone C (28), a five-membered A-ring could be built through a benzylic acid-like rearrangement, forming an unusual 3-oxabicyclo[3.2.1]octan-2,7-dione motif; the C-2 is excluded from the A-ring in the rearrangement process. Wu et al. proposed a novel convergent strategy for limonoid biosynthesis (Scheme 3).

Unusual Natural Products from Mangrove-Associated Microorganisms
Previous chemical investigations of mangrove microbes especially mangrove-associated fungi resulted in the discovery of various bioactive secondary metabolites, including polyketides, terpenes, alkaloids, and peptides with diverse structural features.

Polyketides
Polyketides (PKs) are a large family of secondary metabolites with prominent structural diversity and various biological activities, isolated from diverse organisms. Polyketide synthases (PKSs) catalyze the sequential decarboxylative condensations of acyl-CoA thioesters to form structurally diverse PKs [37]. We direct readers to the fantastic reviews for more information on PKSs [38][39][40][41]. An even-increasing number of PKs from the mangrove-associated microorganisms are being reported.

Coumarins and Isocoumarins
Naturally occurring coumarins and isocoumarins are an essential class of benzopyrene derivatives and are present in remarkable amounts in plants, while only a few are found in microorganisms and animal sources.

Unusual Natural Products from Mangrove-Associated Microorganisms
Previous chemical investigations of mangrove microbes especially mangrove-associated fungi resulted in the discovery of various bioactive secondary metabolites, including polyketides, terpenes, alkaloids, and peptides with diverse structural features.

Polyketides
Polyketides (PKs) are a large family of secondary metabolites with prominent structural diversity and various biological activities, isolated from diverse organisms. Polyketide synthases (PKSs) catalyze the sequential decarboxylative condensations of acyl-CoA thioesters to form structurally diverse PKs [37]. We direct readers to the fantastic reviews for more information on PKSs [38][39][40][41]. An even-increasing number of PKs from the mangrove-associated microorganisms are being reported.

Coumarins and Isocoumarins
Naturally occurring coumarins and isocoumarins are an essential class of benzopyrene derivatives and are present in remarkable amounts in plants, while only a few are found in microorganisms and animal sources.

Unusual Natural Products from Mangrove-Associated Microorganisms
Previous chemical investigations of mangrove microbes especially mangrove-associated fungi resulted in the discovery of various bioactive secondary metabolites, including polyketides, terpenes, alkaloids, and peptides with diverse structural features.

Polyketides
Polyketides (PKs) are a large family of secondary metabolites with prominent structural diversity and various biological activities, isolated from diverse organisms. Polyketide synthases (PKSs) catalyze the sequential decarboxylative condensations of acyl-CoA thioesters to form structurally diverse PKs [37]. We direct readers to the fantastic reviews for more information on PKSs [38][39][40][41]. An even-increasing number of PKs from the mangrove-associated microorganisms are being reported.

Coumarins and Isocoumarins
Naturally occurring coumarins and isocoumarins are an essential class of benzopyrene derivatives and are present in remarkable amounts in plants, while only a few are found in microorganisms and animal sources.

Chromones
The chromone and its derivatives have been identified as the central backbone in several functional organic compounds, with strategic importance in many research and industrial domains. Until now, 85 new chromone derivatives have been identified from the mangrove-associated fungal species.

Azaphilones
Azaphilones or azaphilonoids are a structurally variable family of fungal polyketide metabolites harboring a highly oxygenated pyranoquinone bicyclic core [47]. In recent years, about 31 azaphilones with unusual structures and remarkable bioactivities were reported from mangrove-associated fungi, including genera Aspergillus, Diaporthe, Penicillium, and Talaromyces.
Two new citrinin derivatives, penicitol A (37) and penicitol B (38) ( Figure 12) were identified from Penicillium chrysogenum ML226 obtained from the rhizosphere soil of the mangrove plant Acanthus ilicifolius [48]. The citrinin derivatives are a family of azaphilones, with the first one, namely citrinin, isolated from a P. citrinum strain in 1931 [49]. Penicitol A (37) is the first reported citrinin derivative with an unusual tetracyclic skeleton, and 38 is the first citrinin dimer with a single oxygen bridging center.

Chromones
The chromone and its derivatives have been identified as the central backbone in several functional organic compounds, with strategic importance in many research and industrial domains. Until now, 85 new chromone derivatives have been identified from the mangrove-associated fungal species.

Chromones
The chromone and its derivatives have been identified as the central backbone in several functional organic compounds, with strategic importance in many research and industrial domains. Until now, 85 new chromone derivatives have been identified from the mangrove-associated fungal species.

Azaphilones
Azaphilones or azaphilonoids are a structurally variable family of fungal polyketide metabolites harboring a highly oxygenated pyranoquinone bicyclic core [47]. In recent years, about 31 azaphilones with unusual structures and remarkable bioactivities were reported from mangrove-associated fungi, including genera Aspergillus, Diaporthe, Penicillium, and Talaromyces.
Two new citrinin derivatives, penicitol A (37) and penicitol B (38) ( Figure 12) were identified from Penicillium chrysogenum ML226 obtained from the rhizosphere soil of the mangrove plant Acanthus ilicifolius [48]. The citrinin derivatives are a family of azaphilones, with the first one, namely citrinin, isolated from a P. citrinum strain in 1931 [49]. Penicitol A (37) is the first reported citrinin derivative with an unusual tetracyclic skeleton, and 38 is the first citrinin dimer with a single oxygen bridging center.

Azaphilones
Azaphilones or azaphilonoids are a structurally variable family of fungal polyketide metabolites harboring a highly oxygenated pyranoquinone bicyclic core [47]. In recent years, about 31 azaphilones with unusual structures and remarkable bioactivities were reported from mangrove-associated fungi, including genera Aspergillus, Diaporthe, Penicillium, and Talaromyces.
Two new citrinin derivatives, penicitol A (37) and penicitol B (38) ( Figure 12) were identified from Penicillium chrysogenum ML226 obtained from the rhizosphere soil of the mangrove plant Acanthus ilicifolius [48]. The citrinin derivatives are a family of azaphilones, with the first one, namely citrinin, isolated from a P. citrinum strain in 1931 [49]. Penicitol A (37) is the first reported citrinin derivative with an unusual tetracyclic skeleton, and 38 is the first citrinin dimer with a single oxygen bridging center.   Figure 10. Structures of compounds 32-34.

Chromones
The chromone and its derivatives have been identified as the central backbone in several functional organic compounds, with strategic importance in many research and industrial domains. Until now, 85 new chromone derivatives have been identified from the mangrove-associated fungal species.

Azaphilones
Azaphilones or azaphilonoids are a structurally variable family of fungal polyketide metabolites harboring a highly oxygenated pyranoquinone bicyclic core [47]. In recent years, about 31 azaphilones with unusual structures and remarkable bioactivities were reported from mangrove-associated fungi, including genera Aspergillus, Diaporthe, Penicillium, and Talaromyces.
Two new citrinin derivatives, penicitol A (37) and penicitol B (38) ( Figure 12) were identified from Penicillium chrysogenum ML226 obtained from the rhizosphere soil of the mangrove plant Acanthus ilicifolius [48]. The citrinin derivatives are a family of azaphilones, with the first one, namely citrinin, isolated from a P. citrinum strain in 1931 [49]. Penicitol A (37) is the first reported citrinin derivative with an unusual tetracyclic skeleton, and 38 is the first citrinin dimer with a single oxygen bridging center.

Benzophenones Derivatives
Benzophenones (BPs) are widely distributed NPs possessing a diphenyl ketone moiety [51]. Given the presence of the chemically active carbonyl group, which can efficiently react with a variety of functional groups, diverse novel skeletons such as isobenzofuran, isoindolinone, and 3-dihydro-1H-indene, etc., could be formed. There are 12 members of BPs that are discovered from mangrove-associated fungi.

Macrolides
Macrolides, especially those possessing 10-to 19-membered ring systems, have diversified structural features and constitute a prominent group of active secondary metabolites. Since the discovery of well-known progenitor macrolide antibiotic pikromycin in 1950 and the second generation of macrolides such as azithromycin and clarithromycin, naturally occurring macrolides have been found today due to their diverse structures and promising biological properties [54]. A total of 63 macrolides have been isolated from the mangrove-associated fungi.

Benzophenones Derivatives
Benzophenones (BPs) are widely distributed NPs possessing a diphenyl ketone moiety [51]. Given the presence of the chemically active carbonyl group, which can efficiently react with a variety of functional groups, diverse novel skeletons such as isobenzofuran, isoindolinone, and 3-dihydro-1H-indene, etc., could be formed. There are 12 members of BPs that are discovered from mangrove-associated fungi.

Macrolides
Macrolides, especially those possessing 10-to 19-membered ring systems, have diversified structural features and constitute a prominent group of active secondary metabolites. Since the discovery of well-known progenitor macrolide antibiotic pikromycin in 1950 and the second generation of macrolides such as azithromycin and clarithromycin, naturally occurring macrolides have been found today due to their diverse structures and promising biological properties [54]. A total of 63 macrolides have been isolated from the mangrove-associated fungi.

Macrolides
Macrolides, especially those possessing 10-to 19-membered ring systems, have diversified structural features and constitute a prominent group of active secondary metabolites. Since the discovery of well-known progenitor macrolide antibiotic pikromycin in 1950 and the second generation of macrolides such as azithromycin and clarithromycin, naturally occurring macrolides have been found today due to their diverse structures and promising biological properties [54]. A total of 63 macrolides have been isolated from the mangrove-associated fungi.
Sumalarins A−C (44−46) ( Figure 14) were identified from the cytotoxic extract of Penicillium. sumatrense MA-92 from the rhizosphere of the mangrove Lumnitzera racemose. Notably, they were the unusual and rare examples of sulfur-containing curvularin derivatives isolated for the first time from natural sources [55]. Compounds 44−46 displayed cytotoxic activities against Du145, HeLa, Huh 7, MCF-7, NCI-H460, SGC-7901, and SW1990 cell lines with IC 50 values ranging from 3.8 µM to 10 µM. Compound 44 is likely formed via Michael's addition of 3-mercaptolactate to the double bond ∆ 10,11 of dehydrocurvularin. Esterification or acylation of 45 probably leads to the biosynthesis of 44 and 46 [55].
Sumalarins A−C (44−46) ( Figure 14) were identified from the cytotoxic extract of Pen icillium. sumatrense MA-92 from the rhizosphere of the mangrove Lumnitzera racemose. No tably, they were the unusual and rare examples of sulfur-containing curvularin deriva tives isolated for the first time from natural sources [55]. Compounds 44−46 displayed cytotoxic activities against Du145, HeLa, Huh 7, MCF-7, NCI-H460, SGC-7901, and SW1990 cell lines with IC50 values ranging from 3.8 μM to 10 μM. Compound 44 is likely formed via Michael's addition of 3-mercaptolactate to the double bond Δ 10,11 of dehydro curvularin. Esterification or acylation of 45 probably leads to the biosynthesis of 44 and 46 [55]. Ansamycins are characterized by an aromatic nucleus connected with a polyketide chain back to a nonadjacent position through an amide bond. Hertweck et al. isolated four unusual ansa macrolides, compounds 47-50 ( Figure 15), from Streptomyces sp. HKI0576, a bacterial endophyte separated from the stem of mangrove Bruguiera gymnorrhiza [56]. This was the first report on discovering ansamycins from a plant endophyte. In addition, the degree of "in-built diversification" of these four compounds is unprecedented for complex polyketides. Among them, divergolide A (47) represents an unusual type of ansa macro lide with an unusual branched side chain and a disrupted polyketide backbone. Further more, the tricyclic chromophore is unprecedented for macrolides, and related O-hetero cyclic substructures are only known from aromatic polyketides, such as the nogalamycin aglycone [57] and chaetoxanthone [58]. Divergolide B (48) represents another unusua type of ansa macrolide featuring a novel benzopyran/chromene core as the first congener of 47. In addition, compounds 49 and 50 share substructures with 47 and 48 but feature structurally intriguing tetracyclic scaffolds. Furthermore, the ansa macrolides display sig nificant antimicrobial and cytotoxic activities, probably regulating the immunity of the mangrove tree. Compounds 47-50 are biosynthesized from a common linear polyketide using 3-amino-5-hydroxybenzoic acid (AHBA) as a primer unit. Various reactions, includ ing an optional acyl migration, generate the diverse multicyclic structures [56,59] Figure 15), from Streptomyces sp. HKI0576, a bacterial endophyte separated from the stem of mangrove Bruguiera gymnorrhiza [56]. This was the first report on discovering ansamycins from a plant endophyte. In addition, the degree of "in-built diversification" of these four compounds is unprecedented for complex polyketides. Among them, divergolide A (47) represents an unusual type of ansa macrolide with an unusual branched side chain and a disrupted polyketide backbone. Furthermore, the tricyclic chromophore is unprecedented for macrolides, and related O-heterocyclic substructures are only known from aromatic polyketides, such as the nogalamycin aglycone [57] and chaetoxanthone [58]. Divergolide B (48) represents another unusual type of ansa macrolide featuring a novel benzopyran/chromene core as the first congener of 47. In addition, compounds 49 and 50 share substructures with 47 and 48 but feature structurally intriguing tetracyclic scaffolds. Furthermore, the ansa macrolides display significant antimicrobial and cytotoxic activities, probably regulating the immunity of the mangrove tree. Compounds 47-50 are biosynthesized from a common linear polyketide using 3-amino-5hydroxybenzoic acid (AHBA) as a primer unit. Various reactions, including an optional acyl migration, generate the diverse multicyclic structures [56,59] (Scheme 6).
SW1990 cell lines with IC50 values ranging from 3.8 μM to 10 μM. Compound 44 is likely formed via Michael's addition of 3-mercaptolactate to the double bond Δ 10,11 of dehydrocurvularin. Esterification or acylation of 45 probably leads to the biosynthesis of 44 and 46 [55]. Ansamycins are characterized by an aromatic nucleus connected with a polyketide chain back to a nonadjacent position through an amide bond. Hertweck et al. isolated four unusual ansa macrolides, compounds 47-50 ( Figure 15), from Streptomyces sp. HKI0576, a bacterial endophyte separated from the stem of mangrove Bruguiera gymnorrhiza [56]. This was the first report on discovering ansamycins from a plant endophyte. In addition, the degree of "in-built diversification" of these four compounds is unprecedented for complex polyketides. Among them, divergolide A (47) represents an unusual type of ansa macrolide with an unusual branched side chain and a disrupted polyketide backbone. Furthermore, the tricyclic chromophore is unprecedented for macrolides, and related O-heterocyclic substructures are only known from aromatic polyketides, such as the nogalamycin aglycone [57] and chaetoxanthone [58]. Divergolide B (48) represents another unusual type of ansa macrolide featuring a novel benzopyran/chromene core as the first congener of 47. In addition, compounds 49 and 50 share substructures with 47 and 48 but feature structurally intriguing tetracyclic scaffolds. Furthermore, the ansa macrolides display significant antimicrobial and cytotoxic activities, probably regulating the immunity of the mangrove tree. Compounds 47-50 are biosynthesized from a common linear polyketide using 3-amino-5-hydroxybenzoic acid (AHBA) as a primer unit. Various reactions, including an optional acyl migration, generate the diverse multicyclic structures [56,59]   In 2014, Shen et al., cloned the biosynthetic gene cluster involved in the biosynthesis of the divergolides from the endophytic Streptomyces sp. W112 isolated from Camptotheca acuminata. Following gene disruption, gene overexpression, and bioinformatics analysis, they laid the foundation for further elucidation of the biosynthetic pathway as well as titer improvement [60]. In addition, Zhong et al. [61] conducted genome sequencing, bioinformatics analysis, and further isolations of four new divergolide congeners with a similar endophytic bacteria, Streptomyces sp. from Bruguiera gymnorrhiza. They showed that specialized acyltransferase domains are for selecting extender units, and the branched isobutylmalonyl-CoA is involved. In 2014, Shen et al., cloned the biosynthetic gene cluster involved in the biosynthesis of the divergolides from the endophytic Streptomyces sp. W112 isolated from Camptotheca acuminata. Following gene disruption, gene overexpression, and bioinformatics analysis they laid the foundation for further elucidation of the biosynthetic pathway as well as titer improvement [60]. In addition, Zhong et al. [61] conducted genome sequencing, bioinfor matics analysis, and further isolations of four new divergolide congeners with a similar endophytic bacteria, Streptomyces sp. from Bruguiera gymnorrhiza. They showed that spe cialized acyltransferase domains are for selecting extender units, and the branched isobu tylmalonyl-CoA is involved.
The total synthesis of divergolide A using the ring-closing metathesis (RCM) ap proach was published by Dai et al., in 2012 [62]. Subsequently, Rasapalli et al. synthesized the western section of divergolides C (49) and D (50) and demonstrated the robustness o C4-C5 as an appropriate approach for the further total synthesis of divergolides C and D in 2013 [63]. This chemical method was also conducted for divergolides A and B. Studies on the total synthesis of divergolides A-D using inexpensive, readily available starting materials and simple operations have also been constantly reported in recent years [64][65][66].
A macrocyclic polyketide with an unusual carbon skeleton, namely hainanmycin A (51) (Figure 15), was isolated from Streptomyces sp. 219807 (from mangrove soil collected in Sanya) [67]. Compound 51 featured an unprecedented structural skeleton of a 17-mem bered carbocyclic framework. The cyclo-heptadeca framework containing a cyclopente none ring substituted with a naturally occurring bridgehead enol motif is unique among NPs. It represents a new subgroup, a minor family of carbocyclic polyketide macrolides Hong et al. [67] proposed a plausible biosynthetic pathway for 51 based on the biosynthe sis of akaeolide [68], an analogue of 51. Shortly, the PKS condenses acetyl-CoA and other building units (e.g., methylmalonyl-CoA and malonyl-CoA) to a linearized polyketide backbone. A thioesterase (TE) then releases the backbone with the formation of a δ-lactone Scheme 6. Proposed biosynthetic pathway for compounds 46-49 [56].
The total synthesis of divergolide A using the ring-closing metathesis (RCM) approach was published by Dai et al., in 2012 [62]. Subsequently, Rasapalli et al. synthesized the western section of divergolides C (49) and D (50) and demonstrated the robustness of C4-C5 as an appropriate approach for the further total synthesis of divergolides C and D in 2013 [63]. This chemical method was also conducted for divergolides A and B. Studies on the total synthesis of divergolides A-D using inexpensive, readily available starting materials and simple operations have also been constantly reported in recent years [64][65][66].
A macrocyclic polyketide with an unusual carbon skeleton, namely hainanmycin A (51) (Figure 15), was isolated from Streptomyces sp. 219807 (from mangrove soil collected in Sanya) [67]. Compound 51 featured an unprecedented structural skeleton of a 17-membered carbocyclic framework. The cyclo-heptadeca framework containing a cyclopentenone ring substituted with a naturally occurring bridgehead enol motif is unique among NPs. It represents a new subgroup, a minor family of carbocyclic polyketide macrolides. Hong et al. [67] proposed a plausible biosynthetic pathway for 51 based on the biosynthesis of akaeolide [68], an analogue of 51. Shortly, the PKS condenses acetyl-CoA and other building units (e.g., methylmalonyl-CoA and malonyl-CoA) to a linearized polyketide backbone. A thioesterase (TE) then releases the backbone with the formation of a δ-lactone ring. Further construction of the C-C bonds of C-16/C-12 and C-18/C-2 generate the structural core. Notably, a C-18 aldehyde intermediate (S1) might be involved in the C-18/C-12 carbon bond formation (Scheme 7). ring. Further construction of the C-C bonds of C-16/C-12 and C-18/C-2 generate the structural core. Notably, a C-18 aldehyde intermediate (S1) might be involved in the C-18/C-12 carbon bond formation (Scheme 7

Others
Eight new compounds, streptoglycerides A-H (52-59) ( Figure 16) possessing a unique ring system, were obtained from Streptomyces sp. isolated from a mangrove sample collected on Kosrae Island [69,70]. This is the first report to describe a rare 6/5/5 tricyclic ring system consisting of a glycerol moiety from marine organisms. Streptoglyceride C (54) showed a weak inhibitory effect on nitric oxide production in BV-2 microglia cells. Upon further investigation of the unusual strain, four new compounds, miharadienes A-D (60-63), possessing unique ring systems and a rare diene side chain, were isolated [71]. A plausible biosynthetic pathway of miharadienes and related compounds, streptoglycerides is proposed in the literature (Scheme 8a) [71]. However, the formation of 52-55 from 63 by attacking the nucleophilic hydroxy on the electron-rich furan ring seems inapplicable. Therefore, we proposed an optional pathway for 52-55 (Scheme 8b). In short, the starting lauryl alcohol derivative appears to react with dihydroxyacetone, an oxidation product of glycerol, to form the intermediate int i through Aldol type reaction of the C-4 active methylene of lauryl alcohol derivative with the carbonyl of the dihydrox-Scheme 7. Proposed biosynthetic pathway for compound 51 [67].

Others
Eight new compounds, streptoglycerides A-H (52-59) ( Figure 16) possessing a unique ring system, were obtained from Streptomyces sp. isolated from a mangrove sample collected on Kosrae Island [69,70]. This is the first report to describe a rare 6/5/5 tricyclic ring system consisting of a glycerol moiety from marine organisms. Streptoglyceride C (54) showed a weak inhibitory effect on nitric oxide production in BV-2 microglia cells. Compounds 56-59 showed significant anti-inflammatory activity by inhibiting lipopolysaccharide (LPS)induced nitric oxide (NO) production in Raw 264.7 cells with IC 50 values ranging from 3.5 to 10.9 µM. It should be noted that 57 suppressed the transcription of iNOS and IL-6 without cytotoxicity. ring. Further construction of the C-C bonds of C-16/C-12 and C-18/C-2 generate the structural core. Notably, a C-18 aldehyde intermediate (S1) might be involved in the C-18/C-12 carbon bond formation (Scheme 7

Others
Eight new compounds, streptoglycerides A-H (52-59) ( Figure 16) possessing a unique ring system, were obtained from Streptomyces sp. isolated from a mangrove sample collected on Kosrae Island [69,70]. This is the first report to describe a rare 6/5/5 tricyclic ring system consisting of a glycerol moiety from marine organisms. Streptoglyceride C (54) showed a weak inhibitory effect on nitric oxide production in BV-2 microglia cells. Upon further investigation of the unusual strain, four new compounds, miharadienes A-D (60-63), possessing unique ring systems and a rare diene side chain, were isolated [71]. A plausible biosynthetic pathway of miharadienes and related compounds, streptoglycerides is proposed in the literature (Scheme 8a) [71]. However, the formation of 52-55 from 63 by attacking the nucleophilic hydroxy on the electron-rich furan ring seems inapplicable. Therefore, we proposed an optional pathway for 52-55 (Scheme 8b). In short, the starting lauryl alcohol derivative appears to react with dihydroxyacetone, an oxidation product of glycerol, to form the intermediate int i through Aldol type reaction of the C-4 active methylene of lauryl alcohol derivative with the carbonyl of the dihydrox- Upon further investigation of the unusual strain, four new compounds, miharadienes A-D (60-63), possessing unique ring systems and a rare diene side chain, were isolated [71]. A plausible biosynthetic pathway of miharadienes and related compounds, streptoglycerides is proposed in the literature (Scheme 8a) [71]. However, the formation of 52-55 from 63 by attacking the nucleophilic hydroxy on the electron-rich furan ring seems inapplicable. Therefore, we proposed an optional pathway for 52-55 (Scheme 8b). In short, the starting lauryl alcohol derivative appears to react with dihydroxyacetone, an oxidation product of glycerol, to form the intermediate int i through Aldol type reaction of the C-4 active methylene of lauryl alcohol derivative with the carbonyl of the dihydroxyacetone. Then the hemiketal formation gives the tetrahydrofuran ring, and ether formation forms the other tetrahydrofuran ring. Afterward, the ketal formation by the interaction of the terminal hydroxyethylene with the hemiketal provides the pyran ring and affords the intermediate int ii, which could be further converted into 52-55, possessing a rare 6/5/5 ring system. yacetone. Then the hemiketal formation gives the tetrahydrofuran ring, and ether formation forms the other tetrahydrofuran ring. Afterward, the ketal formation by the interaction of the terminal hydroxyethylene with the hemiketal provides the pyran ring and affords the intermediate int ii, which could be further converted into 52-55, possessing a rare 6/5/5 ring system.

Terpenoids
The new terpenoids from mangrove fungi can be divided into seven groups based on their chemical structures and biosynthetic pathways: monoterpenes, sesquiterpenes, diterpenes, sesterterpenes, triterpenes, and meroterpenes. Sesquiterpenes (138), sesterterpenes (36), and meroterpenes (72) comprise the most significant proportions of new terpenes from mangrove fungi. However, monoterpenes, diterpenes, and triterpenes were rarely isolated from mangrove fungi, and no new skeleton was discovered.

Terpenoids
The new terpenoids from mangrove fungi can be divided into seven groups based on their chemical structures and biosynthetic pathways: monoterpenes, sesquiterpenes, diterpenes, sesterterpenes, triterpenes, and meroterpenes. Sesquiterpenes (138), sesterterpenes (36), and meroterpenes (72) comprise the most significant proportions of new terpenes from mangrove fungi. However, monoterpenes, diterpenes, and triterpenes were rarely isolated from mangrove fungi, and no new skeleton was discovered.
One tricyclic and three spirobicyclic norsesquiterpenoids (64-67) ( Figure 17) were isolated from the endophytic fungus Pseudolagarobasidium acaciicola (from the mangrove Bruguiera gymnorrhiza) [74,75]. Among them, acaciicolin A (64) possesses a previously unknown skeleton with a uniquely connected 6/5/5 ring system and three consecutive oxygenated sp 3 quaternary carbons at C-7, C-8, and C-8a. The norsesquiterpene skeleton of 64 was named "acaciicolane", and was different from the three known sesquiterpene skeletons with 6/5/5 ring systems: cedrane, prezizaane, and zizaane ( Figure 18). Spiroa-  The first total synthesis of 68 and 69 in their racemic forms was reported by Dong e al. in 2021 [78]. The approach featured a rhodium-catalyzed deconstructive formation o a tricyclic skeleton by C-C activation of cyclobutanone derivatives, generating (±)-68 and (±)-69 in 13 and 14 steps with 0.56% and 0.49% overall yields, respectively. In the same year, K Sugita described another more efficient synthetic pathway for the total practica  The first total synthesis of 68 and 69 in their racemic forms was reported by Dong e al. in 2021 [78]. The approach featured a rhodium-catalyzed deconstructive formation o a tricyclic skeleton by C-C activation of cyclobutanone derivatives, generating (±)-68 and (±)-69 in 13 and 14 steps with 0.56% and 0.49% overall yields, respectively. In the same year, K Sugita described another more efficient synthetic pathway for the total practica  The first total synthesis of 68 and 69 in their racemic forms was reported by Dong e al. in 2021 [78]. The approach featured a rhodium-catalyzed deconstructive formation o a tricyclic skeleton by C-C activation of cyclobutanone derivatives, generating (±)-68 an (±)-69 in 13 and 14 steps with 0.56% and 0.49% overall yields, respectively. In the sam year, K Sugita described another more efficient synthetic pathway for the total practica  [77]. An X-ray crystallographic study determined the structure and configuration. The hypothetical biosynthetic pathway starting from cis-farnesyl pyrophosphate (FPP) was proposed (Scheme 10). Notably, Compounds 68 and 69 exhibited selective activity against the plant pathogenic fungus Colletotrichum gloeosporioides (MIC = 1.0 and 0.125 µg/mL, respectively). tons with 6/5/5 ring systems: cedrane, prezizaane, and zizaane ( Figure 18). Spiroacaciico lides A-C (65-67) has a hitherto unobserved 5/6 fused spirobicyclic ring system. The ab solute configurations of the new compounds 64-66 were determined by single-crystal X ray analysis (Cu-Kα radiation). 64-67 could originate from chamigrane endoperoxide A [76] (Scheme 9).  The first total synthesis of 68 and 69 in their racemic forms was reported by Dong e al. in 2021 [78]. The approach featured a rhodium-catalyzed deconstructive formation o a tricyclic skeleton by C-C activation of cyclobutanone derivatives, generating (±)-68 and (±)-69 in 13 and 14 steps with 0.56% and 0.49% overall yields, respectively. In the same year, K Sugita described another more efficient synthetic pathway for the total practica The first total synthesis of 68 and 69 in their racemic forms was reported by Dong et al. in 2021 [78]. The approach featured a rhodium-catalyzed deconstructive formation of a tricyclic skeleton by C-C activation of cyclobutanone derivatives, generating (±)-68 and (±)-69 in 13 and 14 steps with 0.56% and 0.49% overall yields, respectively. In the same year, K Sugita described another more efficient synthetic pathway for the total practical synthesis of (±)-68 and (±)-69 from commercially available 3-ethoxycyclohex-2-en-1-one with 4.0% overall yields for both compounds [79].

Sesterterpenoids
Sesterterpenoids are a relatively small and rare group of terpenoids found in widespread sources. They always possess interesting carbon skeletons, including linear, monocyclic, polycyclic, and miscellaneous. In addition, they exhibit diverse biological activities such as antimicrobial, cytotoxicity, anti-inflammatory, and protein tyrosine phosphatase B inhibition.
The group of She has been dedicated to the search for structurally unique and biologically active compounds from the mangrove plant-derived fungal endophytes. Five sesterterpenoids of three kinds of carbon skeletons, asperterpenoid A (70) (Figure 19 synthesis of (±)-68 and (±)-69 from commercially available 3-ethoxycyclohex-2-e with 4.0% overall yields for both compounds [79].

Sesterterpenoids
Sesterterpenoids are a relatively small and rare group of terpenoids found i spread sources. They always possess interesting carbon skeletons, including linea ocyclic, polycyclic, and miscellaneous. In addition, they exhibit diverse biologica ties such as antimicrobial, cytotoxicity, anti-inflammatory, and protein tyrosine p tase B inhibition.

Meroterpenoids
Meroterpenoids are secondary metabolites with structures consisting of at least two parts: a terpenoid fragment (mainly mevalonate pathway) and a nonterpenoid fragment [83]. The different nonterpenoid moiety based on the biosynthetic pathway, various terpenoid (the length of the terpenoid chain and its cyclization mode), and the tailoring reactions make the chemical diversity of meroterpenoids.
Chermebilaene A (75) (Figure 20), an unprecedented acorane-type sesquiterpene hybridized with an octadecadienoic acid skeleton, together with an unusual orthoester meroterpenoid, chermebilaene B (76) were isolated from the co-culture extract of P. bilaiae MA-267 (from the rhizosphere of the mangrove Lumnitzera racemosa) and P. chermesinum EN-480 (from the fresh tissue of marine red algal Pterocladiella tenuis) [84]. Compound 75 showed potent inhibitory activities against Ceratobasidium cornigerum and Edwardsiella tarda, and may prove helpful as an antibiotic against aquatic or plant pathogens.

Meroterpenoids
Meroterpenoids are secondary metabolites with structures consisting of at least two parts: a terpenoid fragment (mainly mevalonate pathway) and a nonterpenoid fragment [83]. The different nonterpenoid moiety based on the biosynthetic pathway, various terpenoid (the length of the terpenoid chain and its cyclization mode), and the tailoring reactions make the chemical diversity of meroterpenoids.
Chermebilaene A (75) (Figure 20), an unprecedented acorane-type sesquiterpene hybridized with an octadecadienoic acid skeleton, together with an unusual orthoester meroterpenoid, chermebilaene B (76) were isolated from the co-culture extract of P. bilaiae MA-267 (from the rhizosphere of the mangrove Lumnitzera racemosa) and P. chermesinum EN-480 (from the fresh tissue of marine red algal Pterocladiella tenuis) [84]. Compound 75 showed potent inhibitory activities against Ceratobasidium cornigerum and Edwardsiella tarda, and may prove helpful as an antibiotic against aquatic or plant pathogens. Simpterpenoid A (77) (Figure 21), an unconventional meroterpenoid containing a highly functionalized cyclohexadiene moiety with gem-propane-1,2-dione and methylformate groups, was isolated from the fungal strain Penicillium simplicissimum MA-332, obtained from the rhizospheric soil of the mangrove plant Bruguiera sexangular var. rhynchopetala [85]. The intricate polycyclic skeleton is unique in natural sources. Compound 77 exhibited potent inhibitory activity against influenza neuraminidase with an IC50 value of 8.1 nM.

Meroterpenoids
Meroterpenoids are secondary metabolites with structures consisting of at least two parts: a terpenoid fragment (mainly mevalonate pathway) and a nonterpenoid fragment [83]. The different nonterpenoid moiety based on the biosynthetic pathway, various terpenoid (the length of the terpenoid chain and its cyclization mode), and the tailoring reactions make the chemical diversity of meroterpenoids.
Chermebilaene A (75) (Figure 20), an unprecedented acorane-type sesquiterpene hybridized with an octadecadienoic acid skeleton, together with an unusual orthoester meroterpenoid, chermebilaene B (76) were isolated from the co-culture extract of P. bilaiae MA-267 (from the rhizosphere of the mangrove Lumnitzera racemosa) and P. chermesinum EN-480 (from the fresh tissue of marine red algal Pterocladiella tenuis) [84]. Compound 75 showed potent inhibitory activities against Ceratobasidium cornigerum and Edwardsiella tarda, and may prove helpful as an antibiotic against aquatic or plant pathogens. Simpterpenoid A (77) (Figure 21), an unconventional meroterpenoid containing a highly functionalized cyclohexadiene moiety with gem-propane-1,2-dione and methylformate groups, was isolated from the fungal strain Penicillium simplicissimum MA-332, obtained from the rhizospheric soil of the mangrove plant Bruguiera sexangular var. rhynchopetala [85]. The intricate polycyclic skeleton is unique in natural sources. Compound 77 exhibited potent inhibitory activity against influenza neuraminidase with an IC50 value of 8.1 nM. Simpterpenoid A (77) (Figure 21), an unconventional meroterpenoid containing a highly functionalized cyclohexadiene moiety with gem-propane-1,2-dione and methylformate groups, was isolated from the fungal strain Penicillium simplicissimum MA-332, obtained from the rhizospheric soil of the mangrove plant Bruguiera sexangular var. rhynchopetala [85]. The intricate polycyclic skeleton is unique in natural sources. Compound 77 exhibited potent inhibitory activity against influenza neuraminidase with an IC 50  Two new meroterpenoids, penicianstinoids A and B (78 and 79, Figure 21), were obtained from the mangrove-derived fungus Penicillium sp. TGM112 isolated from the mangrove Bruguiera sexangula var. rhynchopetala [86]. Compared with 79, compound 78 represents an austinoid-like meroterpenoid that is reported for the second time [87], in which a carbon−carbon double bond at C-1′−C-2′ was oxidized to a carbonyl group at C-1′−C-2′.  Two new meroterpenoids, penicianstinoids A and B (78 and 79, Figure 21), were obtained from the mangrove-derived fungus Penicillium sp. TGM112 isolated from the mangrove Bruguiera sexangula var. rhynchopetala [86]. Compared with 79, compound 78 represents an austinoid-like meroterpenoid that is reported for the second time [87], in which a carbon−carbon double bond at C-1 −C-2 was oxidized to a carbonyl group at Two new meroterpenoids, penicianstinoids A and B (78 and 79, Figure 21), were ob tained from the mangrove-derived fungus Penicillium sp. TGM112 isolated from the man grove Bruguiera sexangula var. rhynchopetala [86]. Compared with 79, compound 78 repre sents an austinoid-like meroterpenoid that is reported for the second time [87], in which a carbon−carbon double bond at C-1′−C-2′ was oxidized to a carbonyl group at C-1′−C-2′ Compounds 78 and 79 showed growth inhibition activity against newly hatched larvae o Helicoverpa armigera (Hubner) with IC50 values of 200 μg/mL. In addition, 78 and 79 dis played insecticidal activity against Caenorhabditis elegans with EC50 values of 9.4 (±1.0) and 9.9 (±0.0) μg/mL, respectively. Biogenetically, compounds 76-79 are derived from the same intermediate S2, which is produced by the combination of a polyketide intermediate 3,5-dimethylorsellinicacid (DMOA) and the terpenoid precursor farnesyl pyrophosphate (FPP), following by a series of further modifications to generate a profile of meroterpe noids with diverse skeletons bearing polycyclic cores. DMOA-based meroterpenoids ex hibit diverse structures due to the cyclization of the terpenoid moiety, divergence of post cyclization modification reactions, and various tailoring reactions (Scheme 12) [83].  Two hybrid sesquiterpene-cyclopaldic acid metabolites with an unusual carbon scaffold, namely pestalotiopens A and B (80 and 81) (Figure 22), were obtained from the endophytic fungus Pestalotiopsis sp. (from the leaves of the Chinese mangrove Rhizophora mucronate), together with the known phytotoxin altiloxin B [88]. A plausible biosynthetic pathway of 80 and 81 is proposed (Scheme 13). The cyclopaldic acid and altiloxin B were deduced as precursors.
Two new meroterpenoids, penicianstinoids A and B (78 and 79, Figure 21), were ob tained from the mangrove-derived fungus Penicillium sp. TGM112 isolated from the man grove Bruguiera sexangula var. rhynchopetala [86]. Compared with 79, compound 78 repre sents an austinoid-like meroterpenoid that is reported for the second time [87], in which a carbon−carbon double bond at C-1′−C-2′ was oxidized to a carbonyl group at C-1′−C-2′ Compounds 78 and 79 showed growth inhibition activity against newly hatched larvae o Helicoverpa armigera (Hubner) with IC50 values of 200 μg/mL. In addition, 78 and 79 dis played insecticidal activity against Caenorhabditis elegans with EC50 values of 9.4 (±1.0) and 9.9 (±0.0) μg/mL, respectively. Biogenetically, compounds 76-79 are derived from the same intermediate S2, which is produced by the combination of a polyketide intermediate 3,5-dimethylorsellinicacid (DMOA) and the terpenoid precursor farnesyl pyrophosphate (FPP), following by a series of further modifications to generate a profile of meroterpe noids with diverse skeletons bearing polycyclic cores. DMOA-based meroterpenoids ex hibit diverse structures due to the cyclization of the terpenoid moiety, divergence of post cyclization modification reactions, and various tailoring reactions (Scheme 12) [83]. Two new meroterpenoids, penicianstinoids A and B (78 and 79, Figure 21), were obtained from the mangrove-derived fungus Penicillium sp. TGM112 isolated from the mangrove Bruguiera sexangula var. rhynchopetala [86]. Compared with 79, compound 78 represents an austinoid-like meroterpenoid that is reported for the second time [87], in which a carbon−carbon double bond at C-1′−C-2′ was oxidized to a carbonyl group at C-1′−C-2′. Compounds 78 and 79 showed growth inhibition activity against newly hatched larvae of Helicoverpa armigera (Hubner) with IC50 values of 200 μg/mL. In addition, 78 and 79 displayed insecticidal activity against Caenorhabditis elegans with EC50 values of 9.4 (±1.0) and 9.9 (±0.0) μg/mL, respectively. Biogenetically, compounds 76-79 are derived from the same intermediate S2, which is produced by the combination of a polyketide intermediate 3,5-dimethylorsellinicacid (DMOA) and the terpenoid precursor farnesyl pyrophosphate (FPP), following by a series of further modifications to generate a profile of meroterpenoids with diverse skeletons bearing polycyclic cores. DMOA-based meroterpenoids exhibit diverse structures due to the cyclization of the terpenoid moiety, divergence of postcyclization modification reactions, and various tailoring reactions (Scheme 12) [83]. Scheme 12. Proposed biosynthetic pathway for compounds 76-79 [83].
Two hybrid sesquiterpene-cyclopaldic acid metabolites with an unusual carbon scaffold, namely pestalotiopens A and B (80 and 81) (Figure 22), were obtained from the endophytic fungus Pestalotiopsis sp. (from the leaves of the Chinese mangrove Rhizophora mucronate), together with the known phytotoxin altiloxin B [88]. A plausible biosynthetic pathway of 80 and 81 is proposed (Scheme 13). The cyclopaldic acid and altiloxin B were deduced as precursors. Indole terpenoids are structurally diverse meroterpenoids containing an indole ring from tryptophan and cyclic sesquiterpenes or diterpene backbone moiety [83].
Three indole sesquiterpenes, indotertine A (82) [89] and indotertine B (83a/83b) [90] (Figure 23) were discovered from actinomycete Streptomyces sp. CHQ-64 (derived from the rhizosphere soil of reeds). They possess an unusual skeleton with a condensed ring system made up of a tryptophan-derived indole moiety and a sesquiterpene unit, which represents a new subgroup of indole terpenoids combining amino acid and mevalonate pathways. Indotertine B (83a/83b) exists as a pair of rotamers about the N−C(O) bond with a 2:1 ratio, inseparable by HPLC because of the dynamic interconversion. The analysis of the NOESY spectrum implied that the formyl−N-1 amide bond was S-trans in 83a and S-cis in 83b. Compound 83 displays cytotoxic activities against HCT-8 and A549 tumor cell lines with IC 50 values of 6.96 and 4.88 µM. Further chemical investigation of this fungal strain led to the isolation of drimentine I (84) [91], containing a rare heptacyclic skeleton formed via two bridging linkages. The pentacyclic product indotertine A (82) was hypothetically synthesized by iminium-olefin cyclization. In contrast, tetracyclic product drimentine F could take place from amidic nitrogen by nucleophilic addition to the α-position of the indole moiety (Scheme 14). However, cyclization of 84 happened on indol-NH to afford the linkage between C-14 and N-6 of drimentine F. Compound 84 was found to have weak activity against human cervical carcinoma cell line HeLa, with IC 50 values of 16.73 µM.
represents a new subgroup of indole terpenoids combining amino acid and mevalonate pathways. Indotertine B (83a/83b) exists as a pair of rotamers about the N−C(O) bond with a 2:1 ratio, inseparable by HPLC because of the dynamic interconversion. The analysis o the NOESY spectrum implied that the formyl−N-1 amide bond was S-trans in 83a and S cis in 83b. Compound 83 displays cytotoxic activities against HCT-8 and A549 tumor cel lines with IC50 values of 6.96 and 4.88 μM. Further chemical investigation of this funga strain led to the isolation of drimentine I (84) [91], containing a rare heptacyclic skeleton formed via two bridging linkages. The pentacyclic product indotertine A (82) was hypo thetically synthesized by iminium-olefin cyclization. In contrast, tetracyclic product dri mentine F could take place from amidic nitrogen by nucleophilic addition to the α-posi tion of the indole moiety (Scheme 14). However, cyclization of 84 happened on indol-NH to afford the linkage between C-14 and N-6 of drimentine F. Secopaxilline A (85) [92] (Figure 24) is the first example of indole diterpenoid deriv atives possessing a carbon-nitrogen bond cleavage skeleton, which was isolated from me tabolites of the aciduric fungus Penicillium camemberti OUCMDZ-1492 (separated from the soil and mud around the roots of Rhizophora apiculata). A plausible biosynthetic pathway for secopaxilline A (85) from paxilline was postulated, (Scheme 15), and the process ha been conducted by chemical reactions with a 45% overall yield. Paxilline was derived from the common indole-diterpenoid precursor 3-geranylgeranylindole (GGI) derived the rhizosphere soil of reeds). They possess an unusual skeleton with a condensed ring system made up of a tryptophan-derived indole moiety and a sesquiterpene unit, which represents a new subgroup of indole terpenoids combining amino acid and mevalonate pathways. Indotertine B (83a/83b) exists as a pair of rotamers about the N−C(O) bond with a 2:1 ratio, inseparable by HPLC because of the dynamic interconversion. The analysis of the NOESY spectrum implied that the formyl−N-1 amide bond was S-trans in 83a and Scis in 83b. Compound 83 displays cytotoxic activities against HCT-8 and A549 tumor cell lines with IC50 values of 6.96 and 4.88 μM. Further chemical investigation of this fungal strain led to the isolation of drimentine I (84) [91], containing a rare heptacyclic skeleton formed via two bridging linkages. The pentacyclic product indotertine A (82) was hypothetically synthesized by iminium-olefin cyclization. In contrast, tetracyclic product drimentine F could take place from amidic nitrogen by nucleophilic addition to the α-position of the indole moiety (Scheme 14). However, cyclization of 84 happened on indol-NH to afford the linkage between C-14 and N-6 of drimentine F. Compound 84 was found to have weak activity against human cervical carcinoma cell line HeLa, with IC50 values of 16.73 μM. Secopaxilline A (85) [92] (Figure 24) is the first example of indole diterpenoid derivatives possessing a carbon-nitrogen bond cleavage skeleton, which was isolated from metabolites of the aciduric fungus Penicillium camemberti OUCMDZ-1492 (separated from the soil and mud around the roots of Rhizophora apiculata). A plausible biosynthetic pathway for secopaxilline A (85) from paxilline was postulated, (Scheme 15), and the process has been conducted by chemical reactions with a 45% overall yield. Paxilline was derived from the common indole-diterpenoid precursor 3-geranylgeranylindole (GGI) derived Scheme 14. Proposed biosynthetic pathway for compounds 82-84 [89].
Secopaxilline A (85) [92] (Figure 24) is the first example of indole diterpenoid derivatives possessing a carbon-nitrogen bond cleavage skeleton, which was isolated from metabolites of the aciduric fungus Penicillium camemberti OUCMDZ-1492 (separated from the soil and mud around the roots of Rhizophora apiculata). A plausible biosynthetic pathway for secopaxilline A (85) from paxilline was postulated, (Scheme 15), and the process has been conducted by chemical reactions with a 45% overall yield. Paxilline was derived from the common indole-diterpenoid precursor 3-geranylgeranylindole (GGI) derived from geranylgeranyl pyrophosphate (GGPP) and indole-3-glycerol phosphate [93]  The fungus Mucor irregularis, isolated from the fresh inner tissue of the mangrove Rhizophora stylosa, yields three unusual indole-diterpenes, rhizovarin A-C (86-88, Figure  24) [94], which represent the most complex members of the reported indole-diterpenes. Even though the main structural elements resemble those of other reported indole diterpenes, the presence of an unusual acetal linked to a hemiketal (86) or a ketal (87 and 88) unit in an unprecedented 4,6,6,8,5,6,6,6,6-fused indole-diterpene ring system makes them chemically unique. Their structures and absolute configurations were elucidated by spectroscopic analysis, modified Mosher's method, and chemical calculations. For rhizovarin A (86), the biosynthetic pathway may involve more oxidative steps than penitrem A, a known indole-diterpene derived from a paxilline and two isopentenyl-diphosphate units. (Scheme 15) The biosynthetic pathway has been elucidated by reconstitution of the biosynthetic genes in Aspergillus oryzae [95]. Another unusual indole-diterpene, containing a complex 6,8,6,6,6-fused ring system, rhizovarin D (89), was also obtained in this study. NOESY experiments determined the relative configuration for the stereogenic centers of 89. Each isolated compound was evaluated for antitumor activity against HL-60 and A-549 cell lines. Compounds 86 and 87 showed activities against the human A-549 and HL-60 cancer cell lines (IC50 < 10 μM). Scheme 15. Proposed biosynthetic pathway for compounds 86-89 [93].
The fungus Mucor irregularis, isolated from the fresh inner tissue of the mangrove Rhizophora stylosa, yields three unusual indole-diterpenes, rhizovarin A-C (86-88, Figure 24) [94], which represent the most complex members of the reported indole-diterpenes. Even though the main structural elements resemble those of other reported indole diterpenes, the presence of an unusual acetal linked to a hemiketal (86) or a ketal (87 and 88) unit in an unprecedented 4,6,6,8,5,6,6,6,6-fused indole-diterpene ring system makes them chemically unique. Their structures and absolute configurations were elucidated by spectroscopic analysis, modified Mosher's method, and chemical calculations. For rhizovarin A (86), the biosynthetic pathway may involve more oxidative steps than penitrem A, a known indole-diterpene derived from a paxilline and two isopentenyl-diphosphate units. (Scheme 15) The biosynthetic pathway has been elucidated by reconstitution of the biosynthetic genes in Aspergillus oryzae [95]. Another unusual indole-diterpene, containing a complex 6,8,6,6,6-fused ring system, rhizovarin D (89), was also obtained in this study. NOESY experiments determined the relative configuration for the stereogenic centers of 89. Each isolated compound was evaluated for antitumor activity against HL-60 and A-549 cell lines. Compounds 86 and 87 showed activities against the human A-549 and HL-60 cancer cell lines (IC 50 < 10 µM).
Bioassay-guided fractionation of the bacterial strain Erythrobacter sp. SNB-035 (from mangrove sediments) led to the isolation of erythrazoles A and B (90 and 91) [96] (Figure 25). Structurally, 90 and 91 possess an abenzothiazole moiety, which is rare among NPs. Furthermore, 91 arises from four biosynthetic pathways: NRPS, terpene, shikimate, and polyketide. Although combinations of two of the four pathways are common among NPs, four biosynthetic pathways simultaneously involved are extremely rare (Scheme 16).
Mar. Drugs 2022, 20, x A class of pyrazinopyrimidine-type alkaloids, namely pyrasplorines A-C (1 ( Figure 29) were discovered from the fungus Aspergillus versicolor HDN11-84 [1 rasplorine A (105) represents the first compound with spiro-cyclopentane in pyra rimidine-type alkaloids. The cyclopentane moiety is common in terpenes but rare loids and diketopiperazines, and it is only found in maremycins [104]. The stru probably constructed by the condensation of anthranilic acid with diketopiperaz followed by successive steps to yield the key intermediate S3. Then, compound derived from the S3 via a series of reactions [105] ( Scheme 20).

Indole and Isoindole Alkaloids Derivatives
Various mangrove fungi produce indole and isoindole alkaloids with a plethora of biologically active. The indole-terpenes which also belong to meroterpenes have been described in Section 3.2.3.

Indole and Isoindole Alkaloids Derivatives
Various mangrove fungi produce indole and isoindole alkaloids with a plethora of biologically active. The indole-terpenes which also belong to meroterpenes have been described in Section 3.2.3.
Cytochalasan alkaloid usually consists of a 10-(indol-3-yl) group, a macrocyclic ring, and a perhydroisoindolone moiety. Chaetoglobosin is one class of cytochalasan alkaloid. The mangrove endophytic fungus Penicillium chrysogenum V11 afforded two unusual new Chaetoglobosins, penochalasin I and K (111 and 112) [106,107] (Figure 30), with an unprecedented six-cyclic 6/5/6/5/6/13 fused ring system formed by the connection of C-5 and C-2 of the chaetoglobosin class. Additionally, the biomimetic semi-synthesis of 111 and 112 was successfully carried out from the corresponding co-occurrence analogue chaetoglobosin C and chaetoglobosin A, respectively [107]. Compound 112 displayed significant inhibitory activities against Colletotrichum gloeosporioides and Rhizoctonia solani (MICs = 6.13 µM, 12.26 µM, respectively), which was better than those of control carbendazim. It also exhibited potent cytotoxicity against MDA-MB-435, SGC-7901, and A549 cells (IC 50  The typical paraherquamides (PHQs) are prenylated indole alkaloids with diverse ring systems. PHQs are derived from three building blocks: L-tryptophan, acyclic amino acid (either proline, β-methyl proline, or pipecolic acid), and one or two isoprenyl units. Interestingly, compounds 113-115 ( Figure 31) (mangrovamides A-C, isolated from the Penicillium sp. Separated from a mangrove sediment sample of the South China Sea) feature a bicyclo [2.2.2] diazaoctane core and contain the first documented examples of isoprene derived dimethyl γ-pyrone and γ-methyl proline, instead of the usual β-methyl proline in the PHQ family [108]. A plausible biosynthetic pathway starting from L-ornithine to account for the formation of the observed γ-methyl proline is outlined (Scheme 21). Moreover, the X-ray data determined the absolute configuration of all chiral centers in 113. In an activity assay, 115 showed a moderate acetylcholinesterase inhibitory effect with an IC50 value of 58.0 μM.  [108]. A plausible biosynthetic pathway starting from L-ornithine to account for the formation of the observed γ-methyl proline is outlined (Scheme 21). Moreover, the X-ray data determined the absolute configuration of all chiral centers in 113.
In an activity assay, 115 showed a moderate acetylcholinesterase inhibitory effect with an IC 50 value of 58.0 µM. acid (either proline, β-methyl proline, or pipecolic acid), and one or two isoprenyl units. Interestingly, compounds 113-115 ( Figure 31) (mangrovamides A-C, isolated from the Penicillium sp. Separated from a mangrove sediment sample of the South China Sea) feature a bicyclo [2.2.2] diazaoctane core and contain the first documented examples of isoprene derived dimethyl γ-pyrone and γ-methyl proline, instead of the usual β-methyl proline in the PHQ family [108]. A plausible biosynthetic pathway starting from L-ornithine to account for the formation of the observed γ-methyl proline is outlined (Scheme 21). Moreover, the X-ray data determined the absolute configuration of all chiral centers in 113. In an activity assay, 115 showed a moderate acetylcholinesterase inhibitory effect with an IC50 value of 58.0 μM. Interestingly, compounds 113-115 ( Figure 31) (mangrovamides A-C, isolated from the Penicillium sp. Separated from a mangrove sediment sample of the South China Sea) feature a bicyclo [2.2.2] diazaoctane core and contain the first documented examples of isoprene derived dimethyl γ-pyrone and γ-methyl proline, instead of the usual β-methyl proline in the PHQ family [108]. A plausible biosynthetic pathway starting from L-ornithine to account for the formation of the observed γ-methyl proline is outlined (Scheme 21). Moreover, the X-ray data determined the absolute configuration of all chiral centers in 113. In an activity assay, 115 showed a moderate acetylcholinesterase inhibitory effect with an IC50 value of 58.0 μM.    Quinazoline containing indole alkaloids have pyrimidine [2, 1-b] quinazoline and imidazole [1, 2-a] indole groups linked by methylene (and, in some cases, further linked by additional helical Bridges). Two unusual quinazoline-containing indole alkaloids neosartoryadins A and B (121 and 122) ( Figure 33) along with fiscalin C (a known compound to be related to biosynthesis) were identified from the mangrove endophytic fungus Neosartorya udagawae HDN13-313 [110]. Compounds 121 and 122 is a quinazoline-containing indole alkaloid featuring a unique 6/6/6/5 quinazoline ring directly linked to the 6/5/5 imidazolinone ring. 121 and 122 differs from conventional fumiquinazoline alkaloids such as fiscalin C by the unprecedented pyrido[2, 1-b]-quinazoline moiety, which binds to a pyridine (C ring) rather than a pyrimidine ring, in addition to the presence of a unique tetrahydrofuran ring (D ring). It is speculated that 121 and 122 are biosynthesized from Ltryptophan, anthranilic acid (ATA), L-valine, and 2-aminoisobutyric acid (Aib). The un-Scheme 22. Proposed biosynthetic pathway for compounds 116-120 [109].
Quinazoline containing indole alkaloids have pyrimidine [2, 1-b] quinazoline and imidazole [1, 2-a] indole groups linked by methylene (and, in some cases, further linked by additional helical Bridges). Two unusual quinazoline-containing indole alkaloids neosartoryadins A and B (121 and 122) ( Figure 33) along with fiscalin C (a known compound to be related to biosynthesis) were identified from the mangrove endophytic fungus Neosartorya udagawae HDN13-313 [110]. Compounds 121 and 122 is a quinazoline-containing indole alkaloid featuring a unique 6/6/6/5 quinazoline ring directly linked to the 6/5/5 imidazolinone ring. 121 and 122 differs from conventional fumiquinazoline alkaloids such as fiscalin C by the unprecedented pyrido[2, 1-b]-quinazoline moiety, which binds to a pyridine (C ring) rather than a pyrimidine ring, in addition to the presence of a unique tetrahydrofuran ring (D ring). It is speculated that 121 and 122 are biosynthesized from L-tryptophan, anthranilic acid (ATA), L-valine, and 2-aminoisobutyric acid (Aib). The unprecedented C ring was formed by the key intermediate fiscalin C through further modification by oxidation, hydrolysis, water nucleophilic attack, dehydration, deprotonation, and subsequent aldol reaction (Scheme 23).
Streptocarbazoles, the staurosporine analogues with extraordinary cyclic N-glycosidic connections between 1,3-carbon atoms of the glycosyl moiety and two indole nitrogen atoms of the indolocarbazole core, have also been produced by mangrove actinomycetes.
Streptomyces sp. FMA, isolated from mangrove soil collected in Sanya, Hainan Province of China provided streptocarbazoles A (123) and B (124) [111] (Figure 34). Compound 123 was cytotoxic to HL60, A549, P338, and HeLa cells with IC50 values of 1.4, 5.0, 18.9, and 34.5 μM, respectively, while compound 124 was active against P388 and HeLa cells with IC50 values of 12.8 and 22.5 μM, respectively. In addition, it was demonstrated that streptocarbazoles A arrest the HeLa cells in the G2/M phase at 10 μM. A plausible biogenetic pathway of 123 and 124 was postulated (Scheme 24). The indolocarbazole unit (K252c) was derived from tryptophan, while the glycosyl moiety was probably developed from 2-deoxy-D-pyranoglucose. Subsequently, the first cloning and characterization of an indolocarbazole gene cluster isolated from Streptomyces sanyensis FMA were reported. Indolocarbazole biosynthesis was confirmed by gene inactivation and heterologous expression in Streptomyces coelicolor M1152 [112]. Streptocarbazoles, the staurosporine analogues with extraordinary cyclic N-glycosidic connections between 1,3-carbon atoms of the glycosyl moiety and two indole nitrogen atoms of the indolocarbazole core, have also been produced by mangrove actinomycetes.   [110].
Streptocarbazoles, the staurosporine analogues with extraordinary cyclic N-glycosidic connections between 1,3-carbon atoms of the glycosyl moiety and two indole nitrogen atoms of the indolocarbazole core, have also been produced by mangrove actinomycetes.
Streptomyces sp. FMA, isolated from mangrove soil collected in Sanya, Hainan Province of China provided streptocarbazoles A (123) and B (124) [111] (Figure 34). Compound 123 was cytotoxic to HL60, A549, P338, and HeLa cells with IC50 values of 1.4, 5.0, 18.9, and 34.5 μM, respectively, while compound 124 was active against P388 and HeLa cells with IC50 values of 12.8 and 22.5 μM, respectively. In addition, it was demonstrated that streptocarbazoles A arrest the HeLa cells in the G2/M phase at 10 μM. A plausible biogenetic pathway of 123 and 124 was postulated (Scheme 24). The indolocarbazole unit (K252c) was derived from tryptophan, while the glycosyl moiety was probably developed from 2-deoxy-D-pyranoglucose. Subsequently, the first cloning and characterization of an indolocarbazole gene cluster isolated from Streptomyces sanyensis FMA were reported. Indolocarbazole biosynthesis was confirmed by gene inactivation and heterologous expression in Streptomyces coelicolor M1152 [112].

Pyridines
Piericidins feature a 4-pyridinol core linked with a variable methylated polyene side chain. The strain Streptomyces iakyrus SCSIO NS104, isolated from a mangrove sediment sample collected from the Pearl River estuary to the South China Sea, yielded four new piericidin analogues, iakyricidins A-D (125-128) [113]. Iakyricidins B-D (126-128) represent a new subgroup of piericidin with C-C cyclization and double bond rearrangements in the polyene side chain. In addition, oxidized side chain piericidin analogue iakyricidin A (125) displayed potent antiproliferative activity against human renal carcinoma cell lines ACHN cell with an IC50 value of 20 nM. Compound 125 might be derived by oxidative cleavage between C-13 and C-14 of the precursor. In the plausible biosynthetic pathways of 126-128, the most crucial step would be the yet-to-be-identified enzymatic C8-Scheme 24. Proposed biosynthetic pathway for compounds 123 and 124 [111].

Pyridines
Piericidins feature a 4-pyridinol core linked with a variable methylated polyene side chain. The strain Streptomyces iakyrus SCSIO NS104, isolated from a mangrove sediment sample collected from the Pearl River estuary to the South China Sea, yielded four new piericidin analogues, iakyricidins A-D (125-128) [113]. Iakyricidins B-D (126-128) represent a new subgroup of piericidin with C-C cyclization and double bond rearrangements in the polyene side chain. In addition, oxidized side chain piericidin analogue iakyricidin A (125) displayed potent antiproliferative activity against human renal carcinoma cell lines ACHN cell with an IC 50 value of 20 nM. Compound 125 might be derived by oxidative cleavage between C-13 and C-14 of the precursor. In the plausible biosynthetic pathways of 126-128, the most crucial step would be the yet-to-be-identified enzymatic C8-C12 cyclization from the co-occurrence precursor (Scheme 25).
Piericidins feature a 4-pyridinol core linked with a variable methylated polyene side chain. The strain Streptomyces iakyrus SCSIO NS104, isolated from a mangrove sediment sample collected from the Pearl River estuary to the South China Sea, yielded four new piericidin analogues, iakyricidins A-D (125-128) [113]. Iakyricidins B-D (126-128) represent a new subgroup of piericidin with C-C cyclization and double bond rearrangements in the polyene side chain. In addition, oxidized side chain piericidin analogue iakyricidin A (125) displayed potent antiproliferative activity against human renal carcinoma cell lines ACHN cell with an IC50 value of 20 nM. Compound 125 might be derived by oxidative cleavage between C-13 and C-14 of the precursor. In the plausible biosynthetic pathways of 126-128, the most crucial step would be the yet-to-be-identified enzymatic C8-C12 cyclization from the co-occurrence precursor (Scheme 25).
Chemical investigation of the endophytic fungus Campylocarpon sp. HDN13-307, obtained from the root of mangrove plant Sonneratia caseolaris led to the isolation of four new 4-hydroxy-2-pyridone alkaloids, namely campyridones A-D (129-132) [114] (Figure 35), which existed as two pairs of diastereoisomers, featuring an additional C ring between the decalin and pyridone units, represented new ring systems for this family of alkaloids. A plausible biosynthetic pathway for 129-132 is postulated with the co-occurrence ilicicolin H as a critical intermediate. Ilicicolin H is a typical 4-hydroxy-2-pyridone alkaloid which was considered to be biosynthesized via tetramic acids formed by hybridizing a polyketide unit to a tyrosine (Scheme 26). Compound 132 exhibited activity against Hela cells with IC50 values of 8.8 μM. piericidin analogues, iakyricidins A-D (125-128) [113]. Iakyricidins B-D (126-128) repre sent a new subgroup of piericidin with C-C cyclization and double bond rearrangement in the polyene side chain. In addition, oxidized side chain piericidin analogue iakyricidin A (125) displayed potent antiproliferative activity against human renal carcinoma cel lines ACHN cell with an IC50 value of 20 nM. Compound 125 might be derived by oxida tive cleavage between C-13 and C-14 of the precursor. In the plausible biosynthetic path ways of 126-128, the most crucial step would be the yet-to-be-identified enzymatic C8 C12 cyclization from the co-occurrence precursor (Scheme 25). Scheme 25. Proposed biosynthetic pathway for compounds 125-128 [113].
Chemical investigation of the endophytic fungus Campylocarpon sp. HDN13-307, ob tained from the root of mangrove plant Sonneratia caseolaris led to the isolation of four new 4-hydroxy-2-pyridone alkaloids, namely campyridones A-D (129-132) [114] (Figure 35) which existed as two pairs of diastereoisomers, featuring an additional C ring between the decalin and pyridone units, represented new ring systems for this family of alkaloids A plausible biosynthetic pathway for 129-132 is postulated with the co-occurrence ilicic olin H as a critical intermediate. Ilicicolin H is a typical 4-hydroxy-2-pyridone alkaloid which was considered to be biosynthesized via tetramic acids formed by hybridizing a polyketide unit to a tyrosine (Scheme 26). Compound 132 exhibited activity against Hela cells with IC50 values of 8.8 μM.
Talaramide A (134) (Figure 37) is the second example of an alkaloid with a unique oxidized tricyclic system resembling a bird cage, which was obtained from the mangrove endophytic fungus Talaromyces sp [116]. The first example was rubrobramide, obtained from the fungus Cladobotryum ubrobrunnescens [117]. 134 was a PKS-NRPS hybrid metabolite derived from acetyl acid, malonic acid, and L-leucine. A series of polymerizations, cyclizations, rearrangements, and redox reactions finally afforded the unique oxidized tricyclic skeleton of 134 (Scheme 28). Talaramide A (134) (Figure 37) is the second example of an alkaloid with a unique oxidized tricyclic system resembling a bird cage, which was obtained from the mangrove endophytic fungus Talaromyces sp [116]. The first example was rubrobramide, obtained from the fungus Cladobotryum ubrobrunnescens [117]. 134 was a PKS-NRPS hybrid metabolite derived from acetyl acid, malonic acid, and L-leucine. A series of polymerizations, cyclizations, rearrangements, and redox reactions finally afforded the unique oxidized tricyclic skeleton of 134 (Scheme 28).

Conclusions
In this review, we presented the chemical constituents of the mangrove-associated ecosystem and showcased the diversity of the chemical structures, biological activities, chemical syntheses, and (proposed) biosynthetic pathways.
Structurally diverse secondary metabolites play a crucial role in the discovery campaigns for new NP drug pharmacophores. The mangrove ecosystem is producing various structurally novel compounds that could provide a potent compound library for the identification of lead compounds. Herein, we presented a comprehensive review of 134 mangrove-derived NPs with new carbon skeletons, unique ring systems, or uncommon struc-Scheme 28. Proposed biosynthetic pathway for compound 134 [116].

Conclusions
In this review, we presented the chemical constituents of the mangrove-associated ecosystem and showcased the diversity of the chemical structures, biological activities, chemical syntheses, and (proposed) biosynthetic pathways.
Structurally diverse secondary metabolites play a crucial role in the discovery campaigns for new NP drug pharmacophores. The mangrove ecosystem is producing various structurally novel compounds that could provide a potent compound library for the identification of lead compounds. Herein, we presented a comprehensive review of 134 mangrovederived NPs with new carbon skeletons, unique ring systems, or uncommon structural moieties. The majority of them were produced by mangrove-associated microorganisms, and more than 70% were isolated from endophyte fungus, indicating remarkable chemical diversity and interesting bioactivity of the microbial community. The structural novelty and diversity of these metabolites result from the enormous variety of mangrove ecosystems in combination with their potential biosynthetic capabilities. In addition, they display diverse and remarkable biological activities and are frequently reported as antimicrobial and cytotoxic compounds (Tables 1 and 2), which might attract researchers for further investigations toward chemical synthesis and biosynthesis. Mangrove ecosystems are a rewarding source for producing bioactive substances with novel carbon frameworks and discovering drug lead compounds, attracting pharmaceutical scientists for more in vivo and preclinical studies on these compounds.   In conclusion, through this review, we conveyed that (1) the mangrove-associated ecosystem is still an abundant source of bioactive NPs providing leads for drug development, (2) chemical syntheses of several of the mangrove-associated NPs are completed, but more NPs are to be synthesized and more efficient routes are to be developed, and (3) and the biosynthesis of most of the mangrove-associated NPs remain unclear.