Sulfated Galactofucans: An Outstanding Class of Fucoidans with Promising Bioactivities

Fucoidans encompass versatile and heterogeneous sulfated biopolysaccharides of marine origin, specifically brown algae and marine invertebrates. Their chemistry and bioactivities have been extensively investigated in the last few decades. The reported studies revealed diverse chemical skeletons in which l-fucose is the main sugar monomer. However, other sugars, i.e., galactose, mannose, etc., have been identified to be interspersed, forming several heteropolymers, including galactofucans/fucogalactans (G-fucoidans). Particularly, sulfated galactofucans are associated with rich chemistry contributing to more promising bioactivities than fucans and other marine polysaccharides. The previous reports in the last 20 years showed that G-fucoidans derived from Undaria pinnatifida were the most studied; 21 bioactivities were investigated, especially antitumor and antiviral activities, and unique biomedical applications compared to other marine polysaccharides were demonstrated. Hence, the current article specifically reviews the biogenic sources, chemistry, and outstanding bioactivities of G-fucoidans providing the opportunity to discover novel drug candidates.


Introduction
Fucoidans are unique products of marine organisms, specifically sulfated polysaccharides derived from brown algae and marine invertebrates [1]. They have gained great interest in the last few decades from different fields of sciences, including chemistry, biology, medicine, nutrition, and formulations [2][3][4][5]. All this interest is attributed to the diverse physicochemical, chemical, and biological characteristics [6,7]. These characteristics are relatively related to each other and have been studied previously in a wide variety of literature [8][9][10][11][12][13]. Hence, biological investigations are always performed after full chemical and physicochemical characterizations of purified fucoidans [14][15][16][17]. Monosaccharide composition, molecular weight, sulfation pattern, and sulfation content were found to be the most predominant factors that contribute to fucoidans' molecular mechanisms [11,12,18,19].
F-fucoidans are the most simple and regular form of fucoidans, where the fucoidan backbone is composed of α-L-fucose only, i.e., homopolymers of L-fucose. This form is abundantly extracted from marine invertebrates (e.g., sea cucumber) and to a lesser extent from brown algae [39,40]. However, the International Union of Pure and Applied Chemistry (IUPAC) has recently distinguished the sulfated fucans from fucoidans and classified them in a separate class [41]. The second class is the G-fucoidans, which are highly common in brown algae and composed of β-D-galactose in addition to the α-Lfucose in variable ratios [37,42]. Based on previously reported findings, several species of Laminariales and Fucales are richer in G-fucoidans. In addition, the third and other classes of fucoidans are highly heterogeneous with a contribution of several other sugar monomers such as glucose, mannose, rhamnose, xylose, and uronic acids [38,43]. It is also noteworthy to mention that various or a mixture of the different classes of fucoidan oligomers may be found in the same organism extracts or fractions [36,44].
Particularly, G-fucoidans have been more investigated recently and showed an outstanding broad spectrum of bioactivities and stability in response to autohydrolysis in comparison with other classes of fucoidans and marine polysaccharides [45,46]. For instance, they showed antioxidant, antiviral, anti-inflammatory, anti-hyperglycemic, anticoagulant, and antitumor activities [47][48][49] and were promising candidates for prevention and treatment of coronavirus disease 2019 (COVID-19) [50]. The current production status of brown seaweeds is continuously increasing [51], specifically for F-fucoidans are the most simple and regular form of fucoidans, where the fucoidan backbone is composed of α-L-fucose only, i.e., homopolymers of L-fucose. This form is abundantly extracted from marine invertebrates (e.g., sea cucumber) and to a lesser extent from brown algae [39,40]. However, the International Union of Pure and Applied Chemistry (IUPAC) has recently distinguished the sulfated fucans from fucoidans and classified them in a separate class [41]. The second class is the G-fucoidans, which are highly common in brown algae and composed of β-D-galactose in addition to the α-L-fucose in variable ratios [37,42]. Based on previously reported findings, several species of Laminariales and Fucales are richer in G-fucoidans. In addition, the third and other classes of fucoidans are highly heterogeneous with a contribution of several other sugar monomers such as glucose, mannose, rhamnose, xylose, and uronic acids [38,43]. It is also noteworthy to mention that various or a mixture of the different classes of fucoidan oligomers may be found in the same organism extracts or fractions [36,44].
Particularly, G-fucoidans have been more investigated recently and showed an outstanding broad spectrum of bioactivities and stability in response to autohydrolysis in comparison with other classes of fucoidans and marine polysaccharides [45,46]. For instance, they showed antioxidant, antiviral, anti-inflammatory, anti-hyperglycemic, anticoagulant, and antitumor activities [47][48][49] and were promising candidates for prevention and treatment of coronavirus disease 2019 (COVID-19) [50]. The current production status of brown seaweeds is continuously increasing [51], specifically for Saccharina or Laminaria species and Undaria pinnatifida which are major G-fucoidan producers. For instance, the annual Saccharina production was estimated at 5.7 million tons worth USD 330 million and 2.1 million tons of U. pinnatifida worth USD 0.9 billion [52].
Hence, the current review aims at addressing different aspects of G-fucoidans, including biogenic sources, chemistry, and reported bioactivities. Moreover, proposed structureactivity relationships are highlighted. This may help the further investigation and consequences of galactose presence in the fucoidan chemical backbone.
Traces of other sugars may be found, as in the case of Dictyota menstrualis [58] and Sargassum sp. [59]. Nevertheless, the presence of high percentages of glucose, i.e., fucose:galactose:glucose ratio of 1:0.3:0.25, may indicate contamination of the G-fucoidan with laminarin [60]. In such cases, fucoidans are partially purified by ethanol or cetyltrimethylammonium bromide (CTAB) precipitation and not purified by a specific chromatographic method, including anion exchange resin using diethylaminoethyl cellulose (DEAE-C) [61] or affinity chromatography [62].
In addition, previous studies, with the aid of advanced spectral analyses, i.e., 2D NMR (e.g., HMQC, TOCSY, and NOESY) and mass spectrometry, have attempted to reveal many structural features of G-fucoidans of various biogenic sources, including glycosidic linkages, sugar configuration, branching sites, sulfation pattern, and galactose position [6,63,64]. In addition, they could deduce tentative structure bioactivity relationships, as in the case of the anti-inflammatory mechanism of galactofucan isolated from Saccharina japonica [65].
The results of spectral analyses showed that α-L-fucopyranose (Fucp) and β-D-galactopyranose (Galp) are identified mainly, in which Fucp forms the major backbone and is linked via (1→4) and/or (1→3), while the β-D-galactopyranose molecules are found at branching sites, usually at (1→6), as in case of the G-fucoidan isolated from Hormophysa cuneiformis. In addition, the sulfation pattern is variable based on the glycosidic linkages. For instance, sulfate groups may occupy 2-O and 4-O in →3Fucp1→ or 2-O and 3-O in →4Fucp1→, in addition to 2-O in →3,4Fucp1→ [36]. Other models of sulfated galactofucans derived from Sargassum thunbergii were found to possess →3Fucp1→ as a main backbone with a 2-O-sulfated and 2,4-O-disulfated pattern, while the Galp residues interspersed Fucp in the main chain were linked mainly with →6Galp1→ and 4-O sulfation [46]. Moreover, G-fucoidan isolated from S. polycystum was built up mainly of a 4-O sulfated →3Fucp1→ backbone containing single →2Galp1→ residues sulfated similarly at the 4-O position [66]. Several other models are demonstrated in Figure 2 and Table 1 and in relation to their biomedical applications.

Potential Pharmacological Activities
Diversity in fucoidans' chemical structures is always associated with promising and various bioactivities, which are typical with G-fucoidans [11,103]. The following subsection highlight these bioactivities. A systematic review in the Google Scholar and Scopus databases was performed using the keywords "galactofucan*", "bioactivity", and "biomedical". A timeframe was not pre-established; however, the selection criteria were set to include full-length English articles in peer-reviewed journals and articles addressing biological activities along with the chemical properties of galactofucans. Moreover, publications in which the chemical compositions or structures based on previous analyses were consulted in addition to these original articles. Nevertheless, they were not included in the current review if they did not include any biological assessment. We also identified additional sources through manual reference tracing within the selected articles.
Seventy-two articles that evaluated the various bioactivities of galactofucans from brown seaweeds were identified between 2002 and 2021. In general, research on this topic went from 1.

Potential Pharmacological Activities
Diversity in fucoidans' chemical structures is always associated with promising and various bioactivities, which are typical with G-fucoidans [11,103]. The following subsection highlight these bioactivities. A systematic review in the Google Scholar and Scopus databases was performed using the keywords "galactofucan*", "bioactivity", and "biomedical". A timeframe was not pre-established; however, the selection criteria were set to include full-length English articles in peer-reviewed journals and articles addressing biological activities along with the chemical properties of galactofucans. Moreover, publications in which the chemical compositions or structures based on previous analyses were consulted in addition to these original articles. Nevertheless, they were not included in the current review if they did not include any biological assessment. We also identified additional sources through manual reference tracing within the selected articles.
Seventy-two articles that evaluated the various bioactivities of galactofucans from brown seaweeds were identified between 2002 and 2021. In general, research on this topic went from 1.   Our literature survey revealed that galactofucans from 31 brown algal species had shown promising biological activities. Undaria pinnatifida and Saccharina japonica were the most studied species with 17 and 11 publications, respectively. It is also worth noting that the genera Sargassum (10 species) and Saccharina (5 species) presented the highest numbers of species ( Figure 5). Special attention has been paid to sporophylls of U. pinnatifida (mekabu), which is used in Japan and Korean cuisines, as well as in other countries of East Asia [125,126]. Mekabu's fucoidan is a galactofucan with anticancer/antitumor, anticoagulation, antimetastatic, antioxidant, antithrombotic, and antiviral activities [122]. Currently, the company Biocorp, a Korean manufacturer of health food made of naturally derived ingredients, sells mekabu's fucoidan from Jeollanam-do Sea in South Korea [127].  Our literature survey revealed that galactofucans from 31 brown algal species had shown promising biological activities. Undaria pinnatifida and Saccharina japonica were the most studied species with 17 and 11 publications, respectively. It is also worth noting that the genera Sargassum (10 species) and Saccharina (5 species) presented the highest numbers of species ( Figure 5). Special attention has been paid to sporophylls of U. pinnatifida (mekabu), which is used in Japan and Korean cuisines, as well as in other countries of East Asia [125,126]. Mekabu's fucoidan is a galactofucan with anticancer/antitumor, anticoagulation, antimetastatic, antioxidant, antithrombotic, and antiviral activities [122]. Currently, the company Biocorp, a Korean manufacturer of health food made of naturally derived ingredients, sells mekabu's fucoidan from Jeollanam-do Sea in South Korea [127]. The majority of the publications used wild seaweeds for galactofucan extraction. A low number utilized cultivated biomass from open-sea farms, corresponding to either U. pinnatifida (and more specifically the sporophylls) or S. japonica ( Figure 6). Standardizing bioactivity using seaweed cultivars is necessary if this industry wants to develop highvalue markets for functional foods, cosmeceuticals, nutraceuticals, and pharmaceuticals [128]. In the case of G-fucoidans, this would imply more studies using cultivated species to identify cultivars with high bioactivities. The majority of the publications used wild seaweeds for galactofucan extraction. A low number utilized cultivated biomass from open-sea farms, corresponding to either U. pinnatifida (and more specifically the sporophylls) or S. japonica ( Figure 6). Standardizing bioactivity using seaweed cultivars is necessary if this industry wants to develop high-value markets for functional foods, cosmeceuticals, nutraceuticals, and pharmaceuticals [128]. In the case of G-fucoidans, this would imply more studies using cultivated species to identify cultivars with high bioactivities.
Mar. Drugs 2022, 20, 412 9 of 21 low number utilized cultivated biomass from open-sea farms, corresponding to either U. pinnatifida (and more specifically the sporophylls) or S. japonica ( Figure 6). Standardizing bioactivity using seaweed cultivars is necessary if this industry wants to develop highvalue markets for functional foods, cosmeceuticals, nutraceuticals, and pharmaceuticals [128]. In the case of G-fucoidans, this would imply more studies using cultivated species to identify cultivars with high bioactivities.  Bioactivities were mostly evaluated using in vitro tests in most of the publications evaluated (n = 58). Studies involving both in vitro and in vivo (n = 8) and only in vivo tests (n = 5) accounted for fewer publications. Galactofucans showed the highest number of reported bioactivities compared to other classes of polysaccharides in brown, red, and green seaweeds (Figure 7). These findings might support the idea that, among fucoidans, the galactofucan components concentrate the biological activities [24].

Anticancer/Antitumor Activity
Several studies have reported the anticancer/antitumor activities of galactofucans in different cancer cell lines, as well as antiproliferative, antimetastasis, and antiangiogenic effects. For example, mekabu's galactofucans showed antitumor activity against PC-3 (prostate cancer), HeLa (cervical cancer), A549 (alveolar carcinoma), HepG2 (hepatocellular carcinoma), MCF-7 (breast adenocarcinoma), and A-549 (lung carcinoma) cells, in a similar or superior pattern to a commercial fucoidan from Fucus vesiculosus. Structural elucidation of this fucoidan demonstrated an O-acetylated sulfated galactofucan backbone with a fucose:galactose ratio of 1.0:1.1, sulfate (0.97 mol/mol), and acetate (0.24 mol/mol), in addition to the absence of uronic acids. However, this research did not determine any chemical property of the control fucoidan used, i.e., commercial

Anticancer/Antitumor Activity
Several studies have reported the anticancer/antitumor activities of galactofucans in different cancer cell lines, as well as antiproliferative, antimetastasis, and antiangiogenic effects. For example, mekabu's galactofucans showed antitumor activity against PC-3 (prostate cancer), HeLa (cervical cancer), A549 (alveolar carcinoma), HepG2 (hepatocellular carcinoma), MCF-7 (breast adenocarcinoma), and A-549 (lung carcinoma) cells, in a similar or superior pattern to a commercial fucoidan from Fucus vesiculosus. Structural elucidation of this fucoidan demonstrated an O-acetylated sulfated galactofucan backbone with a fucose:galactose ratio of 1.0:1.1, sulfate (0.97 mol/mol), and acetate (0.24 mol/mol), in addition to the absence of uronic acids. However, this research did not determine any chemical property of the control fucoidan used, i.e., commercial fucoidan from F. vesiculosus, and hence, comparison based on structure-activity relationship was not possible [44,117,130], ( Table 2). Another study using Hca-F (mouse hepatocarcinoma) cells showed that the anticancer activity of mekabu might be mediated through the mechanism involving inactivation of the NF-κB pathway mediated by PI3K/Akt and ERK signaling pathways [123]. In addition, Saccharina latissima has been proposed as a more appropriate source of sulfated galactofucan with antitumor activity superior to commercial heparins (Table 2). When compared to F. vesiculosus or other algae species, S. latissima showed lower contents of co-extractable compounds (e.g., phenolic derivatives), a requirement for any potential medical application [107]. Yes. Superior to commercial fucoidan from Fucus for both cancer cell lines [44,117,130] Despite their promising bioactivities, fucoidans' high molecular weight and viscous nature (including galactofucans) may limit their use as therapeutic agents. In this regard, lower-sized fucoidans have emerged as a possible solution to these problems. For example, low-molecular-weight (LMG) mannogalactofucans derived from G-fucoidans of mekabu strongly attenuated the growth of human prostate cancer cells both in vitro and in vivo [116]. Similarly, an LMG sulfated galactofucan from Sargassum thunbergii, with high fucose content, presented better antitumor and antiangiogenic effects against human lung cancer A549 and human umbilical vein endothelial cells, respectively [63].
Our bibliographic search revealed that most of the studies have focused on analyzing the anticancer effect in vitro. So far, only the galactofucans from U. pinnatifida and Sargassum thunbergii have shown promising activities against prostate and lung cancer in vivo, respectively. In both cases, a xenografted mouse model was used to study tumor inhibition [46,116].

Antiviral Activity
Galactofucans show antiviral properties against a number of highly pathogenic viruses, including the human immunodeficiency virus (HIV-1) ( Table 3). They can block the early steps of HIV entry into target cells [84] or inhibit reverse transcriptases [131]. According to [100], the inhibitory activity of fucoidans is specific against viruses that use heparan sulfate as the primary cell receptor. Although all fucans can be considered as potential anti-HIV agents, it seems that galactofucans are the most effective inhibitors among them. Studies have also shown that G-fucoidans present high and selective antiviral activity against herpes simplex virus type 1 and 2 (HSV-1 and HSV-2), showing 50% cytotoxic concentration (CC 50 ) >1000 µg/mL against Vero B cells and IC 50 values in the range 0.7-10.0 µg/mL [78], including both acyclovir (ACV)-sensitive and -resistant strains [85][86][87]. In addition, sulfated xylogalactofucan (F2S2) from Saccharina angustata inhibited the HSV-1 adsorption/attachment to cells with higher potency (0.65 µg/mL) and selectivity index (SI > 1538) than sulfated alginate (0.2-25 µg/mL) from the same species [97]. Moreover, the antiherpetic effect of a commercial sulfated galactofucan from U. pinnatifida has been confirmed in vivo [118]. All these results highlight the importance of the sulfated galactofucans for the prevention of herpetic infections. Table 3. Summarized antiviral activity of G-fucoidans with their respective sources and half-maximal effective or inhibitory concentrations (EC 50 /IC 50 ). Comparisons with antiviral drugs are also shown.
Galactofucans might also be good candidates for preventing and/or treating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for the current COVID-19 pandemic. A recent study showed that G-fucoidans from S. japonica presented a strong bind ability to the virus spike glycoproteins (SGPs), one of the targets for COVID treatment [50]. Furthermore, an in silico study revealed that sulfated galactofucan achieved stable binding with receptor-binding domain (RBD) of SARS CoV-2's spike protein (S-protein) at two sites (sites 1 and 2) [132].

Anti-Inflammatory, Immunomodulatory, and Anticomplement Activities
Jin et al. have studied different factors that may affect the anticomplement activity of Gfucoidans. Among them were extraction methods, molecular weight, fucose:galactose molar ratio, sulfate content, uronic acid, type of glycosidic linkage, branching, and monomeric composition. The study concluded that larger molecular weights were more related to better activities [81]. G-fucoidans might also represent a novel and safer treatment strategy for chronic inflammation or related ailments. Six brown algal species have shown promising anti-inflammatory effects. Galactofucans from Sargassum wightii showed superior activity to aspirin, with EC 90 values ranging from 0.2 to 1.22 mg/mL for inhibition of inflammatoryrelated enzymes [92,93]. Only the galactofucans from Saccharina japonica and Lobophora variegata have been tested in vivo with positive results [42,65,70,104]. Chen et al. showed that the investigated galactofucans from S. japonica were non-cytotoxic in the range of 3.125 to 25 µg/mL [65]. The anti-inflammatory was investigated in the form of fucoidanbased cream using fucoidan derived from F. vesiculosus of fucose:galactose ratio 1.0:0.05. A carrageenan-induced edema model in rats was employed, and the results showed 51-58% inhibition at 50 mg/kg fucoidan, which was comparable to the diclofenac effect. Such an effect was supposed to be linked with inhibition of IL-1β-induced COX-2 expression [133].
The reduction in the generation of nitric oxide (NO) and prostaglandin E2 (PGE2) via the downregulation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) as well as the suppression of pro-inflammatory cytokines tumor necrosis factor (TNF)α and interleukin (IL)-1β production via nuclear factor-kappa B (NF-κB) and mitogenactivated protein kinase (MAPK) have been pointed out as the mechanisms behind the anti-inflammatory activity reported for S. japonica. The fraction (LJNF3) could inhibit the production of 39.7% and 47.08% for TNF-α and IL-1β at 25 µg/mL [65,104]. This species presents a sulfated galactofucan that can be feasibly produced on a large scale due to its low-cost processing and superior anti-inflammatory activity [104]. Interestingly, the reviewed studies on S. japonica have been performed only on cultivated samples, meaning that seaweed cultivars can be good raw materials for anti-inflammatory compounds.
Furthermore, immunomodulatory compounds help to regulate immune function by accelerating or decelerating precise parts of the host response [134]. The complement system, an essential part of innate immunity, plays a pivotal role in eliminating "harmful" substances from the body. However, in some situations, its overactivity leads to diseases such as cancer or heart disease [82]. The immunomodulatory and anticomplement properties of galactofucans have been explored recently. Galactofucans from Saccharina japonica and Lobophora variegata exhibited immune-modulatory effects on RAW 264.7 cells (monocyte/macrophage-like cells) [70,102]. Studies on S. japonica and Sargassum fusiforme suggested that sulfated galactofucans were the active components of the anticomplement activity, with IC 50 values of 4.5 and 5.5 µg/mL, respectively [81]. In addition, G-fucoidans from S. hemiphyllum presented higher anticomplement properties than sulfated galacto-fuco-xyloglucuronomannan from the same species [82]. All in all, galactofucans might be good candidates for immunomodulatory and anticomplement drugs.

Anticoagulant and Antithrombotic Activities
Fucoidans are well-known for their anticoagulant and antithrombotic activities. These polysaccharides have attracted extensive interest in discovering safer anticoagulants, with less hemorrhagic risk and good antithrombotic activity [135]. As part of this complex class of molecules, G-fucoidans also represent a source of potential antithrombotic drugs. For example, a sulfated galactofucan from Spatoglossum schroederi was 2-fold more potent than heparin in stimulating the synthesis of antithrombotic heparan sulfate by endothelial cells of rabbit aorta. In vivo experiments were key to clarifying the antithrombotic activity of this galactofucan, which initially did not show an anticoagulant effect during in vitro experiments. Such an effect was demonstrated for the fraction C at 100 µg/mL with an MW of 24 kDa [73]. Fucoidans can also enhance the plasma level of recombinant tissue plasminogen activator (rtPA), a protein commonly used as a non-interventional treatment to recanalize vessels occluded by acute thrombosis.
Moreover, a galactofucan from U. pinnatifida (specifically from mekabu) showed thrombolytic activity in vivo. This G-fucan's competitive binding in vitro with PA inhibitor (PAI-1), a molecule that quickly neutralizes and inhibits rtPA, was the mechanism underlying fucoidan-mediated thrombolysis. It is worth mentioning that galactofucan from Korean samples showed better thrombolytic activity and binding affinity with PAI-1 than that from Russian samples. The authors suggested that Korean mekabu seemed to synthesize more active galactofucan than its Russian analog [114].
Furthermore, the higher percentage of galactose may also result in higher anticoagulant activity. For instance, Zayed et al. showed that fraction 6 (9% galactose) produced by dye affinity chromatography from F. vesiculosus exhibited a longer coagulation time (thrombin time 66 s) compared to other fractions, i.e., fractions 1 (7.4% galactose) and M (7.5% galactose) with 47 s and 31 s, respectively, at a fucoidan concentration of 10 µg/mL [8].

Antioxidant Activity
The scavenging effect of fucoidans on harmful oxidants, such as superoxide anion, hydrogen peroxide, hydroxyl radicals, and singlet oxygen, has attracted considerable interest from the food and pharmaceutical industries [136]. In this regard, galactofucan from the Tunisian brown seaweed Cystoseira compressa exhibited valuable antioxidant properties when subjected to various antioxidant tests, i.e., ferrous ion chelation, ferric ion reduction, and DPPH radical scavenging assays (Table 4). For instance, the DPPH assay resulted in an IC 50 value of 430 µg/mL compared to 560 µg/mL for sodium alginate isolated from the same organism [64]. Similarly, a galactofucan from Sargassum thunbergii showed a higher scavenging effect of superoxide radical compared to vitamin C (ascorbic acid) [90] (Table 4). However, care should be considered when examining antioxidant activities, since contaminants such as co-extracted secondary metabolites (e.g., phlorotannins), not the galactofucans themselves, might be responsible for the reported bioactivities [1,107].

Other Biological Activities
Two recent studies have reported that galactofucans from Sargassum siliquosum exhibited antilipogenesis properties. According to the authors, the purified G-fucoidans (80 µg/mL) from this species induced a 28.9% reduction in lipid synthesis in human hepatoma cell line HepG2 after being induced by lipid accumulation with 1.0 mM oleate. The study used pioglitazone as a positive control at a concentration of 40 µg/mL [10,89]. In addition, the hypolipidemic effect was reported for a sulfated galactofucan from Saccharina japonica via inhibition of pancreatic lipase activity in a dose-dependent manner. Interestingly, this polysaccharide was not degraded by the human digestive system, likely due to its high molecular weight. Hence, this study might correlate such bioactivity not to the systemic effect, but through modulation of the microbiota composition. These results suggested that galactofucans could serve as fat-reducing health supplements without affecting the total sugar level [105].
In addition, in vitro and in vivo studies have shown antidiabetic and antihypertensive potentials of G-fucoidans from Sargassum wightii. In addition, galactofucans from this species showed superior antidiabetic activities compared to acarbose and diprotein-A (antidiabetic agents). The antidiabetic properties have proven to be significantly higher (p < 0.05) in terms of inhibitory activities for several enzymes involved in glucose metabolism, including α-amylase (IC 90 = 0.9 mg/mL), α-glucosidase (IC 90 = 1.4 mg/mL), and dipeptidyl peptidase-4 (IC 90 = 0.1 mg/mL). In addition, the antihypertensive activity was tested against angiotensin-converting enzyme-I, showing an IC 90 value of 0.2 mg/mL. Furthermore, these studies concluded that this compound was safe for consumption [92,93].
Fibroblast growth factor/fibroblast growth factor receptor (FGF/FGFR) signaling plays an essential role in various biological processes, including tumor growth and angiogenesis, regulation of cell chemotactic response process, cell proliferation, and differentiation [137,138].
Recently, it has been demonstrated that an LMW (10.9 kDa) galactofucan from S. japonica can regulate the FGFR-mediated MAPK signal pathway after incubation of BaF3 cells with 100 µg/mL, in comparison with heparin (2 µg/mL) [101]. Sulfated galactofucans might also represent a good regulator of FGF-1 when compared to the natural ligand, i.e., heparin [106].
In addition, a galactofucan from Sargassum fusiforme has shown promising activity on AD in vivo. During pharmacological experiments, this compound increased the cognitive abilities of scopolamine-, ethanol-, and sodium nitrite-treated mice against memory [80]. Furthermore, the radioprotective effect is a property recently attributed to fucoidans, including galactofucans from S. feldmannii. This species is the most promising source of radiosensitizing compounds among other Sargassum species at a concentration of 40 µg/mL, especially against human colon HT-29 and breast MDA-MB-231 cancer cells. It showed a significant, more than 30%, reduction in colony number of cancerous cells compared to irradiated cells [79,139].

Pharmacokinetic Studies
Despite the limited number of studies discussing the pharmacokinetics of galactofucans, including absorption, tissue distribution, metabolism, and excretion (ADME) behavior, pharmacokinetic study is an essential step for drug development, particularly after oral, topical, and intravenous administration. Such investigations have been included in recent studies and demonstrated highly promising results that qualified fucoidans to be potential candidates for further clinical trials in their pharmaceutical dosage forms [140][141][142].
G-fucoidan-based topical formulations, especially that derived from F. vesiculosus, have recently been employed in animal models such as rats. Following the administration of ointment containing 15% fucoidan at a dose of 50-150 mg/g, fucoidan was reported to be distributed into skin, striated muscle, and plasma with the highest concentration in striated muscle (AUC 0-48 = 2.2 µg·h/g) and without accumulation in plasma during five days of administration [142]. However, there was a literature conflict regarding fucoidan absorption from the digestive tract after oral intake due to its high molecular weight and subsequent mechanism of action, either via a systematic or modulation of gut microbiota composition effect [105,143]. The ELISA competitive antibody assay for sulfated polysaccharide showed low human plasma concentration of G-fucoidans following oral ingestion of 3 g/day for 12 days, where only 4.0 and 12.9 mg/L were resulted from oral ingestion of U. pinnatifida, equivalent to 10% and 75% pure fucoidan, respectively [143]. In addition, it was reported that fucoidan from F. vesiculosus following intragastric administration to the rats was found to be distributed to different organs such as the kidney, spleen, and liver. Interestingly, the kidney showed the maximum concentration, represented by AUC 0-t = 10.7 µg·h/g and C max = 1.2 µg/g after 5 h. In addition, it demonstrated a long absorption time and half-life time with a mean residence time of 6.8 h [140].

Conclusions and Future Perspectives
It has been well documented that fucoidans' bioactivities are affected by four major factors, namely monomeric composition, glycosidic linkages, sulfate ester content, and sulfation pattern. Nevertheless, G-fucoidans or sulfated galactofucans are a unique class of fucoidans chemically and pharmacologically. Several brown seaweed species are recognized as good biogenic resources. They have attracted great attention in the last few years, especially following the great advances in marine biotechnology, chromatography, and spectroscopic techniques. Such advances could allow investigating the heterogeneous chemical composition of fucoidans and confirming the purity of isolated fucoidans. In comparison with other chemical classes of fucoidans, G-fucoidans' chemical diversity has been reported mostly to be accompanied by various and potential pharmacological bioactivities, including antitumor, antiviral, and anticoagulant effects, especially those derived from U. pinnatifida. Previous literature has related some bioactivities with high fucose and sulfate contents or low molecular weight. Yet, the structure-activity relationships and the presence of galactose in higher percentages in the G-fucoidan chemical skeleton have not been revealed clearly. Nevertheless, the authors can assume that the superior bioactivities of G-fucoidans may be attributed to the branched chemical bones since galactose is always found in side chains, which is in agreement with previous literature showing that branched-chain fucoidans always exhibited more promising pharmacological effects. Hence, future studies should address these dark areas of G-fucoidans which can explain the secrets behind their outstanding biological effects. In addition, the seasonal variation and structural differences regarding G-fucoidans should be addressed in relation to bioactivities. The previous investigations on G-fucoidans have also proved their safety for consumption; therefore, G-fucoidans could be developed as a novel functional ingredient in the pharmaceutical and food industries. Furthermore, pharmacokinetic investigations for G-fucoidans should be further specified using different sources and formulations. Such research may help further clinical trials of this outstanding class of fucoidans in different pharmaceutical dosage forms.