An Overview of Potential Seaweed-Derived Bioactive Compounds for Pharmaceutical Applications

Nowadays, seaweeds are widely involved in biotechnological applications. Due to the variety of bioactive compounds in their composition, species of phylum Ochrophyta, class Phaeophyceae, phylum Rhodophyta and Chlorophyta are valuable for the food, cosmetic, pharmaceutical and nutraceutical industries. Seaweeds have been consumed as whole food since ancient times and used to treat several diseases, even though the mechanisms of action were unknown. During the last decades, research has demonstrated that those unique compounds express beneficial properties for human health. Each compound has peculiar properties (e.g., antioxidant, antimicrobial, antiviral activities, etc.) that can be exploited to enhance human health. Seaweed’s extracted polysaccharides are already involved in the pharmaceutical industry, with the aim of replacing synthetic compounds with components of natural origin. This review aims at a better understanding of the recent uses of algae in drug development, with the scope of replacing synthetic compounds and the multiple biotechnological applications that make up seaweed’s potential in industrial companies. Further research is needed to better understand the mechanisms of action of seaweed’s compounds and to embrace the use of seaweeds in pharmaceutical companies and other applications, with the final scope being to produce sustainable and healthier products.


Introduction
Our planet is covered by 70% aquatic habitats, inhabited by a great variety of organisms. Most of them are incredibly important and provide us with ecosystem services such as seafood, raw materials, tourism and cultural heritage. Macroalgae, or seaweeds, provide us with several services; they have long since been used as a nutritional food resource and in traditional medicine [1], but only in the last decades, with advanced technologies, has it been possible to characterize and apply the biological properties of macroalgal compounds for biotechnological purposes. Seaweeds are a source of peculiar compounds with interesting properties that can be useful for pharmaceutical and industrial applications. Due to their non-toxic, edible, cheap and easy culturing properties, macroalgae are optimal candidates for replacing synthetic compounds with those of natural origin.
The trend of eating seaweed-based food has increased as scientific reports have confirmed the antioxidant, antimicrobial and antiviral effects of seaweed metabolites. The potentiality of seaweed can vary depending on the type of algae, harvesting period and environmental conditions; thus, every species has peculiar compounds that can act in different ways, exhibiting diverse properties.
Through the present review, the main seaweed compounds will be discussed, and several study cases will be analysed to prove the beneficial effects of seaweed compounds for human health [2,3]. The most important polysaccharides found in brown seaweeds The study of Gosch et al. [51] shows the variation of fatty acids profiles among b red and green seaweeds, where green seaweeds contain high levels of omega-3 F particular a high amount of ALA (data confirmed by Rohani-Ghadikolaei et al. [52 study reports the biochemical composition of seaweeds from the Persian Gulf. T thors suggest green seaweeds as potential food or feed additive for human and consumption. Moreover, biochemical analyses performed on Arabian Gulf Sea identify Ulva spp. as the most suitable candidates for developing low-fat food PUFA-rich nutraceuticals [53]. Nevertheless, analysis from tropical seaweeds from the Indian coast reveal ophyceae and Rhodophyta as being rich in omega-3 PUFAs and omega-6 PUFAs, r tively, while Chlorophyta are rich in monounsaturated fatty acids (MUFAs) [54].
Khan et al. [55] isolated two anti-inflammatory omega-3 PUFAs of stearidon (SA) and EPA and one pro-inflammatory omega-6 PUFA of arachidonic acid (AA Undaria pinnatifida (Phaeophyceae). Results showed that SA and EPA reduced edem ythema and blood flow potently in induced mouse ear inflammation, suggesting U pinnatifida as remedy for inflammation-related symptoms. Therefore, future stud needed to understand the environmental and seasonal impacts on FA profiles o weeds to obtain the right yield for biotechnological applications.
Marine algae are the most significant sources of non-animal polysaccharide their structures vary according with the type of algae, phase of life cycle of macr when harvested and environmental conditions [56,57]. Polysaccharides (PSs) are hydrate biopolymers consisting of simple sugars linked by glycoside. The functio tivities of PSs have been widely described in the literature as they show powerful b ical activities. For example, isolate PSs (fucoidans) from Mediterranean brown sea showed anti-inflammatory and gastroprotective activities [58]. Porphyran, a sulp carbohydrate derived from red algae of the genus Porphyra showed anti-inflamm antioxidant, antihyperlipidemic, antioxidant and antitumour activities [59-61 munostimulatory activity has been confirmed also in ulvan, a polysaccharide pre green algae [62]. Significant polysaccharides found in marine algae are agar, carrag alginate, fucoidan, laminarin and ulvan [47].
Agar is a strongly gelling seaweed PS with hydrocolloidal properties. The main ture of agar is chemically characterized by the repeating units of D-galactose and hydro-L-galactose, with a few variations, as well as by a low ester sulphate content [ Matsuhashi [65] showed the structure of agar which consists in two groups of PSs: a ( Figure 1), a neutral PS, and agaropectin, an oversimplified term for the charged P Carrageenan, mainly found in red algae, is a linear PS formed from alternati phated or non-sulphated galactose units and linked with α-1,3-glycosidic bond and galactose bonds (Figure 2) [66]. Carrageenans are found in nature in the form of hy as a mix of nonhomologous polysaccharides. Therefore, based on the principal re disaccharide unit and the position at which the sulphate group is connected on the tose unit, carrageenans are divided into three major fractions (κ-carrageenan, ι-car nan and λ-carrageenan) and four minor fractions (θ-carrageenan, ν-carrageenan, ξ geenan and μ-carrageenan) [67,68]. Carrageenan, mainly found in red algae, is a linear PS formed from alternating sulphated or non-sulphated galactose units and linked with α-1,3-glycosidic bond and β-1,4galactose bonds (Figure 2) [66]. Carrageenans are found in nature in the form of hybrids, as a mix of nonhomologous polysaccharides. Therefore, based on the principal repeated disaccharide unit and the position at which the sulphate group is connected on the galactose unit, carrageenans are divided into three major fractions (κ-carrageenan, ι-carrageenan and λ-carrageenan) and four minor fractions (θ-carrageenan, ν-carrageenan, ξ-carrageenan and µ-carrageenan) [67,68]. Fucoidan, alginate and laminarin are PSs found only in brow contain substantial percentages of L-fucose and sulphate ester g complex structures differ from species to species. Besides fucose contain other monosaccharides (mannose, galactose, glucose, xylo ids, even acetyl groups and protein [69]. Alginate is a polysaccharide made of polymers of alginic aci of β-D-mannuronic acid (M) and α-L-guluronic acid (G) joined ( Figure 4). Alginates are anionic linear PSs found in brown seawe naria sp. and Ascophyllum nodosum. Alginate has been reported as b films. Indeed, due to its excellent stabilizing and thickening pro used in food products and medicine [71,73,74].  Fucoidan, alginate and laminarin are PSs found only in brown seaweeds. Fucoidans contain substantial percentages of L-fucose and sulphate ester groups ( Figure 3). Their complex structures differ from species to species. Besides fucose and sulphate, they also contain other monosaccharides (mannose, galactose, glucose, xylose, etc.) and uronic acids, even acetyl groups and protein [69]. Fucoidan, alginate and laminarin are PSs found only in brow contain substantial percentages of L-fucose and sulphate ester g complex structures differ from species to species. Besides fucose contain other monosaccharides (mannose, galactose, glucose, xyl ids, even acetyl groups and protein [69]. Alginate is a polysaccharide made of polymers of alginic ac of β-D-mannuronic acid (M) and α-L-guluronic acid (G) joined ( Figure 4). Alginates are anionic linear PSs found in brown seawe naria sp. and Ascophyllum nodosum. Alginate has been reported as b films. Indeed, due to its excellent stabilizing and thickening pro used in food products and medicine [71,73,74]. Laminarin is a linear polysaccharide made up of β-(1,3)-gluc with repeated units ( Figure 5). It is used in the food industry and bi Alginate is a polysaccharide made of polymers of alginic acid, with monomer units of β-D-mannuronic acid (M) and α-L-guluronic acid (G) joined by 1,4 linkages [70][71][72] ( Figure 4). Alginates are anionic linear PSs found in brown seaweeds, usually from Laminaria sp. and Ascophyllum nodosum. Alginate has been reported as being able to form edible films. Indeed, due to its excellent stabilizing and thickening properties, it is commonly used in food products and medicine [71,73,74].
Mar. Drugs 2022, 20, x FOR PEER REVIEW Fucoidan, alginate and laminarin are PSs found only in brown seaweeds. Fu contain substantial percentages of L-fucose and sulphate ester groups ( Figure 3 complex structures differ from species to species. Besides fucose and sulphate, t contain other monosaccharides (mannose, galactose, glucose, xylose, etc.) and ur ids, even acetyl groups and protein [69]. Alginate is a polysaccharide made of polymers of alginic acid, with monom of β-D-mannuronic acid (M) and α-L-guluronic acid (G) joined by 1,4 linkages ( Figure 4). Alginates are anionic linear PSs found in brown seaweeds, usually fro naria sp. and Ascophyllum nodosum. Alginate has been reported as being able to form films. Indeed, due to its excellent stabilizing and thickening properties, it is com used in food products and medicine [71,73,74]. Laminarin is a linear polysaccharide made up of β-(1,3)-glucan with β-(1,6)-b with repeated units ( Figure 5). It is used in the food industry and biomedicine becau various bio-functional properties when modified trough chemical processes [75]. Fo ple, laminarin has immunostimulatory effects that can activate macrophages, leadin healing of wounds and antitumour properties [76]. An enhanced antioxidant activit observed when this laminarin is irradiated with gamma rays [77].
Ulvan is a PS common in green algae. The ulvan backbone is most made up o β-(1,4)-linked monosaccharides that can vary (rhamnose, xylose, glucuronic acid a ronic acid), with characteristic repeating disaccharide units [78] (Figure 6). Even Laminarin is a linear polysaccharide made up of β-(1,3)-glucan with β-(1,6)-branches with repeated units ( Figure 5). It is used in the food industry and biomedicine because of its various bio-functional properties when modified trough chemical processes [75]. For example, laminarin has immunostimulatory effects that can activate macrophages, leading to the healing of wounds and antitumour properties [76]. An enhanced antioxidant activity is also observed when this laminarin is irradiated with gamma rays [77].  Polyphenols are compounds which contain one or more aromatic rings droxyl groups, and they have been shown to exhibit antioxidant, antimicrob betic, anti-inflammatory and anticancer properties in in vitro and in vivo stud antioxidant activity and redox potential of polyphenols allow them to reduce oxygen species (ROS) involved in several human disorders [83]. Antioxidant extracted from seaweeds are already available on the market for their health for their ability to prolong food expiring [84].
Among several classes of algal polyphenols, phlorotannins are the mo they have shown pharmacologically potential. According to the variety of str ages between phloroglucinol units (1,3,5-trihydroxybenzene) and the number groups present, phlorotannins are divided into six specific groups: phloretho fucols, fucophlorethols, eckols and carmalols [85] (Figure 7).
A huge group of natural products commonly found in plant and mari terpenoids. They are a unique class of algae secondary metabolites compris Ulvan is a PS common in green algae. The ulvan backbone is most made up of αand β-(1,4)-linked monosaccharides that can vary (rhamnose, xylose, glucuronic acid and iduronic acid), with characteristic repeating disaccharide units [78] (Figure 6). Even though the potential of ulvan needs to be better acknowledged, some studies already prove its potential in pharmaceutical and industrial sectors [62,[79][80][81].  Polyphenols are compounds which contain one or more aromatic ring droxyl groups, and they have been shown to exhibit antioxidant, antimicro betic, anti-inflammatory and anticancer properties in in vitro and in vivo stu antioxidant activity and redox potential of polyphenols allow them to reduc oxygen species (ROS) involved in several human disorders [83]. Antioxidan extracted from seaweeds are already available on the market for their heal for their ability to prolong food expiring [84].
Among several classes of algal polyphenols, phlorotannins are the m they have shown pharmacologically potential. According to the variety of st ages between phloroglucinol units (1,3,5-trihydroxybenzene) and the numbe groups present, phlorotannins are divided into six specific groups: phloreth fucols, fucophlorethols, eckols and carmalols [85] (Figure 7).
Diverse research focuses on the isolation and properties of phlorotann of them possess antimicrobial [86], antioxidant [87], anti-HIV [88], antiprol anticancer [90], anti-inflammatory, antidiabetes [91], anti-Alzheimer diseas tensive [92] and radioprotective [93] properties. The presence of phlorotann is limited to brown algae and their number and functions depend on the cond (size, age, type of algae), environment (light, water temperature, salinity, sea Polyphenols are compounds which contain one or more aromatic rings bearing hydroxyl groups, and they have been shown to exhibit antioxidant, antimicrobial, antidiabetic, anti-inflammatory and anticancer properties in in vitro and in vivo studies [82]. The antioxidant activity and redox potential of polyphenols allow them to reduce the reactive oxygen species (ROS) involved in several human disorders [83]. Antioxidant compounds extracted from seaweeds are already available on the market for their health benefits or for their ability to prolong food expiring [84].
Among several classes of algal polyphenols, phlorotannins are the most known as they have shown pharmacologically potential. According to the variety of structural linkages between phloroglucinol units (1,3,5-trihydroxybenzene) and the number of hydroxyl groups present, phlorotannins are divided into six specific groups: phlorethols, fuhalols, fucols, fucophlorethols, eckols and carmalols [85] (Figure 7).
Diverse research focuses on the isolation and properties of phlorotannins, as many of them possess antimicrobial [86], antioxidant [87], anti-HIV [88], antiproliferative [89], anticancer [90], anti-inflammatory, antidiabetes [91], anti-Alzheimer diseases, antihypertensive [92] and radioprotective [93] properties. The presence of phlorotannins in nature is limited to brown algae and their number and functions depend on the condition of algae (size, age, type of algae), environment (light, water temperature, salinity, seasonality) and ecosystem (nutrient levels, magnitude of herbivory) [94]. A literature revision performed by Hannan et al. [97] updated information on the health effects of seaweed phytosterols, compounds with high reputation in therapeutic activity for their cholesterol-lowering potential [98]. Moreover, phytosterols of marine algae, particularly fucosterol, have been investigated for health benefits, including anti-diabetes, anti-obesity, anti-Alzheimer's, antiaging, anticancer and hepatoprotection, among many others.
Nevertheless, for edible algae, it is important to consider heavy metals present in their biomass. Heavy metals discharged in aquatic environments can damage marine species and could be dangerous for human health through their accumulative behaviour and toxicity [99]. Toxic elements such as Al, Pb, Hg, Cd and As can be damaging even at low levels when ingested over a long period of time. The same is true for an excessive intake of essential metals [100].
The quantity of toxic metals as Al, Cd, Pb and Hg has been determined in European and Asian edible seaweeds. Al was the major toxic metal found (57.5 mg Al/kg dry weight), followed by Pb (0.40 mg/kg dw), with the highest average concentration found in seaweed salad from Asia (Undaria pinnatifida, Laminaria ochroleuca, Himanthalia elongata). The highest average concentration of Hg was recorded in Laminaria ochroleuca (the European kombu). As for Cd, it is worth mentioning the concentration recorded in Undaria pinnatifida (wakame) from Asia of 0.59 mg/kg dw [101], probably due to the high levels of industry in the Asian countries.
The regulation of the European Commission (EC) n° 488/2014 [102] defined the maximum limit only for Cd on algae (3 mg/kg), but no limits are defined for Al and Pb in seaweeds [103].
However, French legislation sets maximum levels for Cd (0.5 mg/kg dw) and for Pb (5 mg/kg dw) in edible French algae [101]. Roleda et al. [104] detected the presence of heavy metals in Saccharina latissima and Alaria esculenta (brown algae) and Palmaria palmata (red alga) from natural population and aquaculture of North Europe. Data suggest a concentration of heavy metals below the upper limits set by the French recommendation and the EU Commission Regulation on contaminants in food products, identifying these algae as not harmful for human consumption.
However, there is a need for guidelines regarding the limit and quality of seaweed consumption, with a general European regulation that establishes the maximal concentration of heavy metals in seaweeds, to not exceed seaweed consumption and preserve our health. A huge group of natural products commonly found in plant and marine algae are terpenoids. They are a unique class of algae secondary metabolites comprising terpenes attached to an additional functional group, usually containing oxygen. Several studies suggest that these compounds may be employed for treatment of cancer cells as well as for mammary, skin, lung, forestomach, colon, pancreatic and prostate carcinomas cells, being open to development of new cancer therapies [95,96]. Terpenoids are commonly classified as monoterpenoids, sesquiterpenes, diterpenes, sesterterpenes based on their chemical structure, which may vary from species to species.
A literature revision performed by Hannan et al. [97] updated information on the health effects of seaweed phytosterols, compounds with high reputation in therapeutic activity for their cholesterol-lowering potential [98]. Moreover, phytosterols of marine algae, particularly fucosterol, have been investigated for health benefits, including antidiabetes, anti-obesity, anti-Alzheimer's, antiaging, anticancer and hepatoprotection, among many others.
Nevertheless, for edible algae, it is important to consider heavy metals present in their biomass. Heavy metals discharged in aquatic environments can damage marine species and could be dangerous for human health through their accumulative behaviour and toxicity [99]. Toxic elements such as Al, Pb, Hg, Cd and As can be damaging even at low levels when ingested over a long period of time. The same is true for an excessive intake of essential metals [100].
The quantity of toxic metals as Al, Cd, Pb and Hg has been determined in European and Asian edible seaweeds. Al was the major toxic metal found (57.5 mg Al/kg dry weight), followed by Pb (0.40 mg/kg dw), with the highest average concentration found in seaweed salad from Asia (Undaria pinnatifida, Laminaria ochroleuca, Himanthalia elongata). The highest average concentration of Hg was recorded in Laminaria ochroleuca (the European kombu). As for Cd, it is worth mentioning the concentration recorded in Undaria pinnatifida (wakame) from Asia of 0.59 mg/kg dw [101], probably due to the high levels of industry in the Asian countries.
The regulation of the European Commission (EC) n • 488/2014 [102] defined the maximum limit only for Cd on algae (3 mg/kg), but no limits are defined for Al and Pb in seaweeds [103].
However, French legislation sets maximum levels for Cd (0.5 mg/kg dw) and for Pb (5 mg/kg dw) in edible French algae [101]. Roleda et al. [104] detected the presence of heavy metals in Saccharina latissima and Alaria esculenta (brown algae) and Palmaria palmata (red alga) from natural population and aquaculture of North Europe. Data suggest a concentration of heavy metals below the upper limits set by the French recommendation and the EU Commission Regulation on contaminants in food products, identifying these algae as not harmful for human consumption. However, there is a need for guidelines regarding the limit and quality of seaweed consumption, with a general European regulation that establishes the maximal concentration of heavy metals in seaweeds, to not exceed seaweed consumption and preserve our health.

Seaweeds in Pharmaceutical Studies and Applications
Due to the extensive exploitation of terrestrial biological resources, it is fundamental to find new resources for human health research and drug development. Along the years, the research on marine drugs has obtained important achievements; several studies assess in vitro and in vivo tests to explore biological compounds of marine organisms, with the aim of evaluating their mechanisms of action and exploiting them for pharmaceutical purposes. On this ground, research holds on to this new branch of pharmacy, the marine pharmacology, which involves the integration of multiple disciplines and requires the comprehensive understanding and application of multiple knowledge [126].
Before pharmacological tests, it is necessary to verify the potential of seaweed bioactive compounds. Through standardization and quality control of parameters, it is possible to have a clear spectrum of the components included in seaweeds, which will be correctly identified and tested. Such studies will ensure the identity, quality and efficacy of biological compounds [127]. Examples of pharmacognostic analysis are reported for Chaetomorpha antennina, Ulva lactuca [128], Sargassum wightii [129] and Sargassum cinereum [130].
The study of pharmacokinetics profiling of active molecules is an essential step in drug development. Pharmacokinetics studies how a drug acts after administration via the processes of absorption, distribution, metabolism and excretion. The understanding of the pharmacokinetics of marine-derived polysaccharides has led to their potential use in pharmaceutical formulations [131].
Although the number of reports about new marine-derived compounds has increased, only a few have been reported a pharmacokinetic pathway. For example, Pozharitskaya et al. [132] reported the pharmacokinetics of fucoidan from Fucus vesiculosus after oral administration to rats. Other examples are given by Arunkumar et al. [133], who reported on the pharmacokinetic profiling of in vitro seaweed sulphated polysaccharides against Salmonella typhi. Shannon et al., [134] have shown seaweed's bioactive compounds after oral administration acts as prebiotics and positively modulates the gut microbiota. Ventura et al. [135] reported on pharmacokinetic studies that evidence safety after Fucus vesiculosus polysaccharides oral absorption.
Topical applications have several advantages compared to the pathway of oral administration: the pharmacokinetics are based on skin absorption; this avoids extensive first-pass metabolism and provides direct access and localization at the site of action. Topical applications are usually well-tolerated and can be an alternative for patients who cannot use other administration routes [136,137]. Pozharitskaya et al. [138] investigated the pharmacokinetics after topical application of fucoidans from Fucus vesiculosus. Data suggest that a significant amount of fucoidan-based drug was retained in the skin after the topical dose and accumulated in the striated muscle. Single doses (50-150 mg/kg) and multiple doses (100 mg/kg over five days) of topical application caused no signs of dryness, erythema, hemorrhage, edema or erosion/excoriation in skin rats. The kinetics of topical application of fucoidan have been also investigated by Obluchinskaya et al. [139] to evaluate the anti-inflammatory activity of fucoidan extracted from Fucus vesiculosus in carrageenan-induced rat paw edema. The fucoidan-based cream dose-dependently inhibited paw edema after topical application, with an efficacy at higher doses equal to diclofenac gel, a synthetic anti-inflammatory drug used to treat pain and inflammatory diseases. Results suggest that fucoidan release was controlled by drug diffusion into the skin, thus, the topical application of fucoidan-based cream might be an effective method to treat inflammation and skin diseases.
Therefore, a deep characterization of pharmacokinetics can give us useful information to better understand the molecular basis behind the pharmacological activity, to correct doses and treatment, obtaining more selective drug applications [131]. In Table 2, several cases of in vitro and in vivo studies are reported to confirm the potential of seaweeds bioactive compounds in medicine and pharmaceutical applications. Undaria pinnatifida Antitumoral activity on Malme-3M, SiHa cells [143] Undaria pinnatifida

Pre-Clinical Study Pharmaceutical Property Reference
Caulerpa racemosa, C. scalpelliformis

In vivo
Anti-inflammatory activity [190,191] Ulva lactuca Sulphated PSs Anticoagulant activity [199] Ulva rigida Ethanolic extracts Anti-hyperglycaemic activity [200] Caulerpa mexicana Methanolic extracts Anti-inflammatory activity [201] 3.1. Phylum Ochrophyta, Class Phaeophyceae Phaeophyceae are predominantly brown in colour due to their content of carotenoid fucoxanthins. These algae are recognized as an important source of bioactive compounds and other elements beneficial for human health. Fucoxanthin is an orange-coloured xanthophyll pigment [202,203] found in high content in Phaeophyceae, Haptophyta, Bacillariophyceae, Chrysophyceae and, to a lesser extent, in Rhodophyta, Raphidophyceae and Dinophyta.
Those pigments not only give the algae their peculiar colour, but they also exhibit several biological activities such as anti-inflammatory [204,205], anti-obesity [206,207], antiangiogenic [208] and anticancer properties which can be exploited for pharmaceutical purposes. In vivo and in vitro assays showed inhibition of tumour growth in lung cancer due to fucoxanthin isolated from Laminaria japonica [140], while fucoxanthin isolated from the marine alga Ishige okamurae inhibited B16-F10 melanoma cells implanted in albino mice [168]. Chung et al. [141] isolated fucoxanthins from the brown alga Laminaria japonica with the intent to evaluate their effect on in vitro metastasis models of B16-F10 melanoma cells. Tests showed a significant reduction in metastasis; the further step was testing fucoxanthins in vivo on lung metastasis. In conclusion, Ching et al. [141] demonstrated a significant inhibition in lung cancer cells' growth, proving that fucoxanthins might be value in preventing cancer metastasis [141]. Atya et al. [142] isolated fucoxanthin from Colpome-nia sinuosa and Sargassum prismaticum to evaluate in vitro anticancer activity and in vivo antioxidant activity. HCT-116 (colon adenocarcinoma), MCF-7 (breast adenocarcinoma) and HepG-2 (liver adenocarcinoma) cell lines showed a reduction in growth, while the in vivo tests evaluated the hepatoprotective ability of fucoxanthin in mice. The results were positive, as the antioxidant and anti-inflammatory properties of the extract protected the hepatocytes from inflammation and membrane damage in mice [142]. Wang et al. [143] investigated the cancer cell growth inhibition of fucoxanthins extract from Undaria pinnatifida. Among the cancer cell lines investigated, melanoma Malme-3M and cervix squamous SiHa cells carcinoma showed major growth inhibition [143]. Although in vitro results are positive, it is necessary to improve the studies performing in vivo tests. The correlation between antioxidant activity and total phenolic content or fucoxanthin has been as well demonstrated by Yan et al. [209]. Results showed higher antioxidant activity in Sargassum horneri and Cystoseira hakodatensis compared to Eisenia bicyclis, Kjellmaniella crassifolia and Alaria crassifolia. This could be explained by the higher total phenolic content and fucoxanthin in Sargassum horneri and Cystoseira hakodatensis [210].
Among PSs, fucoidans have exhibited several pharmacological properties. The structure and composition of fucoidans vary among different species of brown algae, thus the beneficial effects they provide also vary [211]. Several fucoidans express antiproliferative activity against cancer cells [105]. Boo et al. individuate anticancer activities in fucoidan from Undaria pinnatifida against human lung adenocarcinoma cell line A549 [144]. Shibata et al. [106] evaluated the action of fucoidans extracted from Cladosiphon okamuranus, a type of edible algae, during Helicobacter pylori infection, confirming the antimicrobial activity. The mechanism of defence is due to the inhibition of urease enzyme avoiding the Helicobacter pylori adhesion to the gastric mucosa. Palanisamy et al. [107] isolated fucoidan from Spatoglossum asperum to detect antimicrobial activity against Aeromonas hydrophila (Gram-negative bacteria) using agar bioassay. In vitro antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, resulting in a reduced power and total antioxidant activities. Results showed that fucoidan extracts exhibit antioxidant properties in a dose-dependent manner, as well as for antibacterial properties [107]. Antibacterial properties were also investigated with crude fucoidans and purified fucoidans from the brown algae Fucus vesiculosus. For both fucoidan preparations, a bacteriostatic effect was observed on Escherichia coli, Staphylococcus epidermidis, Staphylococcus aureus and Bacillus licheniformis, with Escherichia coli being the most sensitive to each of the fucoidans. Results showed that the purification of fucoidan lead to a change of monosaccharide composition with a decrease in the sulphate and uronic acid contents, that generate a decrease in its antimicrobial activity [108]. In another study performed by Liu et al. [109], crude fucoidans from Laminaria japonica exhibited no antibacterial activity, while depolymerized compounds possessed good antibacterial activity both against Escherichia coli and Staphylococcus aureus. Depolymerized fucoidans combine with the membrane proteins and cause a membrane-disrupting effect, leading to the collapse of membrane structure and eventually resulted in cell death. Therefore, further studies that correlate antimicrobial activity and structure of fucoidans must be performed and better elucidated. Antiviral effects of PSs have been assessed against herpes virus strains. Native fucoidan from Fucus evanescens and its derivative were tested against Herpes Simplex Virus type 1 (HSV-1), Herpes Simplex Virus type 2 (HSV-2), enterovirus (ECHO-1) and human immunodeficiency virus (HIV-1). The investigation of both native fucoidan with nonregular structure and derivates can give us the answer of which structural fragment of these unique polysaccharides is important against viruses. In vitro assays were performed in African green monkey kidney (Vero) cells; for in vivo tests, intravaginal HSV-2 infection was induced in female mice. In vitro results showed the ability of both fucoidans to increase the resistance to virus, directly affecting the cell and inhibiting early stage of virus replication. Comparative analysis of antiviral activities of crude and fragmented fucoidans showed that crude fucoidans more effectively inhibits the replication of both types of HSV. Antiviral activity against ECHO-1 and HIV-1 has been detected but is lower than HSV strains. Fur-thermore, in vivo studies showed that intraperitoneal administration of fucoidans in mice protected the animals from lethal intravaginal HSV-2 infection [110]. The antiviral effect against HSV-1 and HVS-2 was also individuated from sulphated polysaccharide isolated from Sargassum patens [145,146], where it has been detected an increase of anti-HSV activity with increasing sulphate ester content of polysaccharides [212]. These results suggest the feasibility of inhibiting HSV infection with seaweeds polysaccharide having specific structure against the virus.
Pozharitskaya et al. [111] elucidated the anti-coagulant bioactivities of the high molecular weight of fucoidan from Fucus vesiculosus in several in vitro model, which has been confirmed by de Azevedo et al. [112], as well. The anticoagulant activity of fucoidan has been also detected in in vivo assay, where human volunteers consumed 3 g for 12 days of oral administration of fucoidan from Undaria pinnatifida [113].
Fucoidans have been discovered being active against diabetes issues. Fucoidans isolated from Undaria pinnatifida were investigated against three starch hydrolysing enzymes, α-amylase, α-glucosidase and amyloglucosidase. It was demonstrated that while the fucoidan extract exhibited significant inhibitory effects against all the three starch hydrolases, it exhibited significantly stronger inhibitory effects against α-glucosidase, suggesting fucoidans from Undaria pinnatifida as an antidiabetic agent [114]. Jia et al. [169] investigated PSs structural characterization and antidiabetic activity from Sargassum fusiforme and Macrocystis pyrifera in high-fat diet and streptozotocin-induced diabetic rats. Oral administration of PSs restrained loss of weight and increased water intake, significantly controlling the increase of levels of blood glucose, triglyceride and total cholesterol in diabetic rats. Therefore, polysaccharides from brown seaweeds could be promising candidates as natural medicines and functional foods for the improvement of diabetes problems.
Polyphenols are other powerful compounds with interesting biological activities. Extracted from Padina australis found to inhibit microbial growth by damaging the cytoplasmic membrane and destroying the cell bacteria. Phenols and flavonoids attack the phosphate group leading to the breakdown of phospholipid molecules of the cell bacteria into carboxylic acid, glycerol and phosphoric acid. As a result, the growth of bacteria is retarded and they eventually die [147]. Chkhikvishvili and Ramazanov [147] already demonstrated the bactericidal potential of phenolic extracted from Padina australis on Bacillus cereus; Kumar et al. [148], through in vitro bioassay, showed inhibition against beta-lactamase negative Escherichia coli ATCC 25922, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus cereus by phenolic extracts of Padina australis, revealing that this species possess narrow-spectrum antibacterial activity.
Nagayama et al. [86] examined the bactericidal effects of crude and purified phlorotannins from the brown alga Ecklonia kurome on pathogenic bacteria. Phlorotannins showed bactericidal activity against Staphylococcus aureus, Streptococcus pyogenes, Bacillus cereus, Campylobacter fetus, Campylobacter jejuni, Escherichia coli, Salmonella enteritidis, Salmonella typhimurium and Vibrio parahaemolyticus. Moreover, the effect post administration of phlorotannins on male and female mice was investigated to confirm the safety of phlorotannins for mammals. The experiment proves the antibacterial activity of phlorotannins from Ecklonia kurome. These results may suggest the addition of these compounds in food product or to develop drug with antibacterial activity with no harmful effects on our organisms. Lee et al. [162,163] analysed the antimicrobial activity of extract from Ecklonia stolonifera. It showed that dieckol purely isolated from Ecklonia stolonifera evidenced the antibacterial activity against methicillin-resistant Staphylococcus aureus and methicillin-susceptible Staphylococcus aureus, with MIC values ranging from 32 to 64 µg/mL.
The multiple activities of phlorotannins were already exploited in the traditional Korean medicine, where Sargassum hemiphylum was already used for the treatment of various allergic diseases. Na et al. [158] stated the significant contribution of Sargassum hemiphylum to the treatment of atopic allergic reactions, such as atopic dermatitis. Dioxinodehydroeckol (DHE) and phlorofucofuroeckol A (PFF-A) isolated from Ecklonia stolonifera contribute to attenuate allergic reactions and might be a promising candidate for the design of novel inhibitor allergic reaction [164]. In vivo assay on rats fed with Eisenia arborea showed inhibition of IgE and anti-degranulation of histamine, suggesting that Eisenia arborea might possess anti-allergic effects [170].
The antioxidant activity of phlorotannins has been detected in brown seaweeds to treat neurodegenerative diseases such as Alzheimer's. It has been proved that the inhibition of AChE enzyme, which catalyses the breakdown of ACh, is a useful therapeutic approach for the symptomatic treatment of Alzheimer's disease. The anticholinesterase activity of seaweed extracts has been tested against acetylcholinesterase (AChE) and butyryl cholinesterase (BChE), which are the main enzymes of Alzheimer's disease [213]. Among the tested seaweeds, Fucus spiralis, Bifurcaria bifurcata and Cystoseira stricta had potential anticholinesterase activity, which could be used in the future as therapeutic agents for Alzheimer's disease. Phlorotannins of the South African seaweed Dictyota humifusa were extracted to test acetylcholinesterase inhibitory activity; results showed that the extracts were effective at inhibiting AChE [159]. Moreover, the inhibitory effect on AChE and BChE activities measured by Custodio et al. [149] suggests a possible therapeutic value of phenols present in Cystoseira tamariscifolia and Cystoseira nodicaulis, as they are reported as potential anticholinesterase inhibitors. Further screening of AChE and BChE inhibitory activity on ethanol extracts of different seaweeds showed that extracts from Cystoseira usneoides and Fucus spiralis were potent inhibitors [214]. It has also been found that phenol found in Ecklonia maxima, Ecklonia stolonifera and Ishige okamurae were also able to inhibit AChE [91,150], as well as dieckol and phlorofucofuroeckol, two phlorotannins found in brown algae Eisenia sp. and Ecklonia sp. [165].
Phlorotannin-rich fractions extracted from Cystoseira sedoides (PHT-SED), Cladostephus spongeosis (PHT-CLAD) and Padina pavonica displayed in vivo antioxidant activity among all three species, with higher activity for Cystoseira sedoides. The study demonstrated that phlorotannins have a strong antioxidant activity towards radicals, showed by the anti-inflammatory assays resulting in a reduction of paw edema and ear thickness in a dosedependent way in mice tested. The anti-inflammatory potential of phlorotannins is also due to the inhibition of oxidative stress by decreasing the production of malondialdehyde (MDA), which is manifested with an increase in free radicals [215]. Kim and Kim [157] reported an anti-inflammatory effect from phloroglucinol, a monomer of phlorotannins, derived from Ecklonia cava. The antioxidant mechanism of phloroglucinol can be attributed to three hydroxyl groups existed in phloroglucinol that can react with ROS.
Phlorotannins may also be implied in cosmetics, as their compounds possess the ability to prevent the skin aging process due to the inhibition of hyaluronidase, an enzyme able to degrade the hyaluronic acid present in the extracellular matrix. Phlorotannins derivatives such as fucophloroethol, fucodiphloroethol, fucotriphloroethol, 7-phloroeckol, phlorofucofuroeckol and bieckol/dieckol extracted from Cystoseira nodicaulis exhibited hyaluronidase activity, becoming potential candidates for the production of antiaging creams [171]. Dieckol, eckol, bieckol, and phlorofucofuroeckol A extracted from Eisenia bicyclis and Ecklonia kurome also exhibited potent inhibition towards hyaluronidase [166].
Phlorotannins extracted from Ecklonia cava and Ecklonia stolonifera also provided photoprotection towards UV rays by reducing the cell damage caused by solar radiation, being good candidate for the development of sun creams protection [160,161]. 7-phloroeckol and dieckol isolated from the brown algae Ecklonia cava had higher inhibitory activity against tyrosinase, an enzyme linked with the melanin hyperpigmentation of skin. These compounds showed higher inhibitory activity than those of commercial inhibitors such as arbutin and kojic acid [161,167]. The inhibition of tyrosinase plays an important role in skin related disease, indeed tyrosinase inhibitors are already used as a whitening agent in cosmetics and clinical skin treatments [216].

Phylum Rhodophyta
Red algae favour intertidal and subtidal zones of rocky coasts, and many of them are located deeper than brown and green algae, in cold and temperate areas [217]. In the last decade many in vitro and in vivo experiments have confirmed the pharmaceutical potential of red algae extracts [218][219][220]. Phycoerythrins of red algae possess interesting biological activities; Sekar and Chandramohan [221] describe the antitumoural effect of those pigments in mouse tumour cells and human liver carcinoma cells. Other effects reported include antioxidant [33,222] and antidiabetic [222], making phycoerythrins a good alternative in marine pharmaceutical [32].
Red seaweeds are the sole source of certain valuable polysaccharides, namely agar and carrageenan. Their interesting biological properties may be applied in pharmaceutical and medical applications.
Carrageenans differ from structure and type, and each show different biological effects [223]. The antiviral activity of carrageenan from Gigartina skottsbergii against intraperitoneal murine HSV-1 and HSV-2 infection has been proved [121]. Matsuhiro et al. [172] extracted polysaccharide from Schizymenia binderi to evaluate in vitro antiviral activity against HSV-1 and HSV-2 by a plaque reduction assay in Vero cells. Results express the inhibition of the strains, in a similar way that iand k-carrageenans isolated from Gigartina skottsbergii express their antiherpetic activity [121,176]. Talarico and Dalmonte [224] demonstrate λ-carrageenan and ι-carrageenan as potent inhibitors of dengue virus type 2 (DENV-2) and type 3 (DENV-3). Pterocladia capillacea and Laurencia obtusa were investigated for their antiviral activity against Hepatitis C Virus in vitro; hepatocellular carcinoma HepG2 cell and peripheral blood cells were infected with the virus. Results showed antiviral activity for both species, but polysaccharides from Laurencia obtusa were revealed to be the most inhibitor [10].
Seaweed polysaccharides possess promising antiviral properties regarding a broad spectrum of the activity, as well as the complex mode of action. Therefore, seaweeds represent an interesting candidate for further antiviral development, as well as for antimicrobial treatment. Kulshreshtha et al. [179] describe the combined effects of selected extracts of two red seaweeds, Chondrus crispus and Sarcodiotheca gaudichaudii with two well-used antibiotics (tetracycline and streptomycin) against Salmonella Enteritidis. Both in vitro and in vivo tests proved enhanced antimicrobial activity. In vivo assays were performed by ingestion of algal extracts by infected hens, while in vitro tests involved combination with seaweeds extracts and sub-lethal dose of tetracycline and streptomycin [177,178].
Antitumoral potential of carrageenans has been also widely evaluated. Carrageenans extract from the genera Gigartina, Chondrus, Eucheuma and Iridaea spp., which showed that κ-carrageenan significantly prevent in vitro growth of fibroblasts, HeLa cells and mammary cells [174]. i/ε carrageenans extracted from red seaweed Gigartina pistillata demonstrate in vitro potential against colorectal cancer stem cells-like cells [175]. The anticancer effect of carrageenan varies based on the molecular weight of the compound; Khotimchenko et al. [225] reviewed several in vitro and in vivo assays regarding the antitumour potential of native carrageenans and oligocarrageenans. In most cases, oligocarrageenans show higher activity then native carrageenans.
Lins et al. [184] evaluated both in vivo and in vitro antitumoural effect of polysaccharides extracted from Champia parvula. In vivo tests showed the antitumoural effect with sarcoma 180 ascites tumour cells implanted subcutaneously into mice. Moreover, the cytotoxicity of these polysaccharides was tested in vitro against proliferation of HL-60 (human leukaemia), MDA-MB-435 (melanoma), SF-295 (brain), and HCT-8 (human colon) cell lines. The data demonstrate that polysaccharides from Champia parvula inhibited sarcoma 180 tumour growth in mice, but had no effects on human cells proliferation in vitro at the tested concentrations. Furthermore, when the mice were treated simultaneously with both the sulphated polysaccharide and the chemotherapeutic agent fluorouracil (5-FU), the tumour inhibition rate increased significantly [184]. This is a very interesting finding to improve the efficacy of anticancer therapy, developing efficient combination of chemotherapeutic drugs, whereas the side effects are reduced. Zhou et al. [123] prove the antitumoural activity of extracts from Chondrus ocellatus on H-22 tumour cells implanted on mice. The ingestion of a mixture of L-carrageenan and 5-FU results in an enhanced antitumour activity. 5-Fu is usually included in chemotherapy treatments due to their suppression of many tumour cells. Unfortunately, also useful cells (e.g., immune cells) are destroyed by this immunosuppressive. Thus, the implementation of natural substances in chemo-treatments may reduce the side effects [123,226,227]. Finding new natural sources with anticancer activities might give the possibility to develop efficient combination of chemotherapeutic drugs, where the side effects are reduced.
Carrageenans also possess anticoagulant activity, as Andrade et al. [173] described in their in vitro study. Solieria filiformis carrageenans were tested to detect anticoagulant effects. The extracts were assessed by both in vitro activated partial thromboplastin time (APTT) and prothrombin (PT) tests. Results showed a reduction and inhibition of thrombin and factor X in blood coagulation, making Solieria filiformis a good candidate for the development of anticoagulant natural drugs [173]. Although seaweeds extracts are not potent as heparin, other studies confirm the anticoagulant potential of carrageenans [228][229][230][231].
These peculiar compounds are already available in the market as potent food supplements. Moreover, their biological activity gives benefits to health. The ingestion of ι-carrageenan extracted from Sarconema filiforme [181] and κ-carrageenan extracted from Kappaphycus alvarezii [182] were investigated in vivo. Results showed that both carrageenans decreased body weight, systolic blood pressure, abdominal fat, liver fat and plasma total cholesterol concentrations in mice models. The assumption of polysaccharides derived from Gracilaria lemaneiformis showed as well anti-obesity activities in obese hamsters, with a decrease in the weight of adipose tissue, body and liver and lower plasma leptin, total cholesterol and triglyceride levels. Obesity increases cardiovascular risk by elevating blood sugar levels and lowering insulin concentration. The positive results given by red algae polysaccharides in decreasing blood glucose, improving glucose tolerance, increasing levels of C-peptide and liver glycogen, might suggest that these compounds might be useful for the development of anti-obesity and antidiabetic drugs [183,232].
Antioxidant activities have been investigated in extract from the Vietnamese red seaweed Laurencia dendroidea using DPPH, nitric oxide radical scavenging and metal chelating assays. The antidiabetic activity was tested in vivo on mice. The extract of Laurencia dendroidea showed strong α-glucosidase inhibitory and DPPH radical scavenging activities. Methanolic concentrations affected both α-glucosidase inhibitory and antioxidant activities. The seaweed extract was fractionated using different solvents (n-hexane, chloroform, ethyl acetate, butanol, and water); ethyl acetate fraction had the highest inhibitory activities against α-glucosidase inhibition and the strongest antioxidant activities. It also significantly reduced blood glucose level in diabetic mice compared with the diabetic control group [185].
Methanolic extract of the red seaweeds Spyridia filamentosa, Grateloupia lithophila and Hypnea musciformis was evaluated against α-amylase and α-glucosidase by spectrophotometric assays. The strongest inhibitor effect to α-amylase and α-glucosidase was shown by that crude methanolic extract of Spyridia filamentosa, which could have anti-diabetic potential through inhibition of α-amylase and α-glucosidase [180].
Carrageenan and agar are found to be used in food industry due to their capacity to form gels and increase viscosity of aqueous solutions [119,[233][234][235]. Although, their interesting properties make agars suitable for pharmaceutical purposes. Extraction of agar-type polysaccharide from Gracilaria dominguensis possesses potent in vivo antitumour activity by inducing apoptosis and inhibiting the transplantation of Ehrlich ascites carcinoma in mice [118]. Indeed, agar oligosaccharides may be used as an alternative or complementary drug for people with diseases [236].

Phylum Chlorophyta
Chlorophyta possess high quantity of organic compounds that are interesting for pharmaceutical applications. For example, Ripol et al. [196] detected anti-inflammatory activities for five species of green seaweeds, such as Chaetomorpha linum, Rhizoclonium riparium, Ulva intestinalis, Ulva lactuca and Ulva prolifera. All species present inhibition to cyclooxygenase-2 (COX-2), the enzyme responsible for inflammation. Although, the screen of the compounds in the extract has not been performed, future research should focus on the extraction of the bioactive compounds from green macroalgae [196]. There are few studies on the anti-inflammatory activity of green seaweeds, ranging from in vitro assays to in vivo models. In vivo study performed by Bitencourt et al. [201] saw the treatment of mice with ulcerative colitis induced with methanolic extract of Caulerpa mexicana. As results show, the clinical signs observed amelioration in ulcerative colitis. The decreased level of cytokines observed could be associated with reduction in tissue damage found in the colon of the animals treated with algal extracts. Therefore, this extract appears promising for research on metabolites that may be helpful for anti-inflammatory treatment [201]. Different authors investigated the neuroprotective effects and anti-inflammatory activities of Ulva conglobata methanol extracts in hippocampal neuronal HT22 cells and mouse microglial BV2 cells. In this case, Ulva conglobata methanol extracts inhibited both iNOS, decreasing the production of free radicals and COX-2 expressions in microglia, as well as protecting hippocampal neurons against glutamate toxicity [198].
It is likely that the anti-inflammatory effects may be triggered by the alkaloid caulerpin or polysaccharides. During the in vivo assay performed by de Souza et al. [190], individuals of Swiss albino mice were pre-treated with caulerpin from Caulerpa racemosa briefly before the pain induction. Formalin-pain induction was performed with an injection of 20 µL of a 2.5% (v/v) solution of formalin in saline. Results indicate a high anti-inflammatory activity on mice [190]. Lucena et al. [191] tested the anti-inflammatory activity of caulerpin from Caulerpa racemosa to reduce leukocyte recruitment in vitro on zymosan-induced peritonitis model and in vivo on DSS-induced ulcerative colitis mice. The submitted doses of caulerpin were 40 and 4 mg/kg of body weight. Successfully, caulerpin extracts present positive results in anti-inflammatory potential both in vitro and in vivo experiments. The 4 mg/kg dose was able to attenuate weight loss and clinical signs, reduce colon size and decrease the levels of Th1 and Th17 pro-inflammatory cytokines.
Zbakh et al. [195] recently investigated the anti-inflammatory and anticancer activity of dichloromethane and acetone/methanol extracts of the green alga Codium decorticatum. Results revealed that dichloromethane extract and acetone/methanol extract dramatically inhibited the expression of the pro-inflammatory cytokine in endothelial cells. Furthermore, dichloromethane extract showed cytotoxic activity against the HeLa cell line (cervix cancer cells) by inducing apoptosis at the highest concentrations, suggesting Codium decorticatum as potential candidate in therapeutic use for inflammatory diseases and cancer treatments.
The polysaccharide ulvan from Ulva lactuca was proven to express anti-influenza A virus (IAV) activity [124], while the alkaloid caulerpin from Caulerpa sp. demonstrated antiviral effects on bovine viral diarrhea virus (BVDV) [187]. Viability test performed in Vero cells showed that caulerpin from Caulerpa racemosa has promising antiviral activity against HSV-1. The control test was performed with Aciclovir, common antiherpetic medication. In vitro tests showed that caulerpin inhibits the replication of the virus in early stages compared with Aciclovir [189]. The anti-HSV-1 activity has been confirmed in Caulerpa brachypus; different fractions of polysaccharides showed not only inhibition at the first stages of HSV-1 but also the penetration into host cells [192].
The activity of caulerpin was evaluated for its antimicrobial activity against Mycobacterium tuberculosis strain H37Rv. Results indicate that caulerpin may be useful as a lead compound for the development of novel anti-tuberculosis agents, thus, future studies may determine the mechanism of action for tuberculosis inhibition [188]. Agbaje-Daniels et al. [237] investigated the antimicrobial activity of five green seaweeds, namely Ulva fasciata, Ulva lactuca, Chladophora vagabunda, Caulepa taxifolia, Chaetomorpha antennina and Chaetomorpha linum. Extracts of each alga were tested against Bacillus subtilis, Streptococcus pneumoniae, Streptococcus faecalis, Gram negative species Escherichia coli (clinical and laboratory strain), Escherichia coli NCTC 10418, Pseudomonas aeruginosa, Salmonella typhi (clinical strain), Salmonella typhi NCTC 8385 and Klebsiella pneumoniae. It was observed that extracts of Ulva fasciata inhibited strains of Staphylococcus aureus, Bacillus subtilis and Mycobacterium aurium as well as the Escherichia coli strains tested, confirming the multidrug resistance of Ulva fasciata [238].
Results by Shanmughapriya et al. [239] report inhibition of the growth of Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus feacalis and Escherichia coli being tested with Halimeda opuntia extracts. Seaweeds belonging to Halimeda spp. have been investigated for several properties, as well as antioxidant, antibacterial and larvicidal activity [240]. Moreover, methanolic extracts from the macroalga Halimeda tuna showed antimicrobial activity on Staphylococcus aureus, Salmonella typhimurium, Salmonella paratyphi, Klebsiella oxytoca, Escherichia coli and antifungal activity against Aspergillus niger, Aspergillus flavus, Alternaria alternaria, Candida albicans and Epidermophyton floccossum, while isolated diterpenes exhibited antiviral activity on murine coronavirus strain A5Y [186,197].
Saeed et al. [194] tested chloroform extracts of Ulva lactuca and Ulva fasciata for antimicrobial activity against pathogenic bacteria (Klebsiella pneumoniae and Proteus mirabilis) and fungi (Aspergillus flavus, Aspergillus fumingatus and Aspergillus niger). The antibacterial activity was revealed for all strains; as the GC-MS chromatogram indicates, phenolic derivatives are the major constituents of the extracts responsible for the antimicrobial against tested pathogens. These extracts were also tested on human tumour cell lines: hepatocellular carcinoma (HepG2), mammary gland (MCF7), epithelioid Carcinoma (Hela) and human prostate cancer (PC3). Ulva lactuca extract showed strong cytotoxic activity against MCF-7, Hela cell lines, while Ulva fasciata extract had strong cytotoxic activity against PC3 and HepG2 cell lines.
An in vivo test performed injecting polysaccharides from Ulva lactuca into the caudal vein of rats proved inhibition of venous thrombus formation. The compound showed 56% reduction in the weight of the thrombus formed with polysaccharides (20 µg/g of rat weight), while the administration of heparin (1.5 µg/g of rat weight) reduced the thrombus weight of 92% [199]. This study evidences the anticoagulant potential of these sulphated PSs isolated from Ulva lactuca, making an exciting option for future investigation for anticoagulant drugs [199]. Therefore, Ulva spp. can be exploited for diverse purposes; extract of Ulva rigida showed anti-hyperglycaemic effect in experimental diabetes. Diabetes is usually accompanied by increased production of ROS and/or impaired antioxidant defence systems [241]. ROS can cause DNA strand breaks [242] and micronuclei, leading potentially serious consequences for the cell well-functioning [243,244]. Celikler et al. [200] performed in vivo assay to evaluate the potential genotoxic/antigenotoxic effect of Ulva rigida ethanolic extract injected in twenty-four male Wistar rats with induced diabetes mellitus. Extract from Ulva rigida showed genotoxic and/or cytotoxic effect, but also it is effective in reducing the chromosome damage induced by the diabetes [200].
Anticancer activity was evaluated also in fibroblast-like cells (L929) of C3H/HeJ mice connective tissue. In vitro tests with tumour cells and ulvan extracted from Ulva lactuca proved the cytotoxicity activity of this compound [81]. Antitumor in vitro tests have been conducted Caulerpa racemosa and Caulerpa scalpelliformis against human hepatoma cancer cells Huh-7 and human cervical cancer cells HeLa. The high contents of phenolic and flavonoid compounds present in Caulerpa spp. [245] allows these species to express several bioactivities, among this antitumoural activity [195].
Therefore, Caulerpa spp. and Ulva spp. are suggested as valuable source for functional food with a potential to be also used in pharmaceutical applications.

Use of Seaweeds in Traditional and Modern Pharmacology
The use of seaweeds for alimentary and medicinal purposes has been common since ancient times, especially in traditional medicine in Asian countries [246,247] even before the mechanisms of action of their compounds were acknowledged.
For example, the crude extract of the Chinese brown seaweed Sargassum naozhouense has been used to treat fever, infections, laryngitis and other ailments by the local population [248], while species of Kappaphycus and Eucheuma genera are used in Vietnamese medicine to reduce the occurrence of tumors, ulcers and headaches. Sargassum is used for treating iodine deficiency disorders such as goitre [249].
Valuable information regarding the use of Sargassum sp. was found in ancient transcript dated between the years 25-1061 AD. Most of the transcripts have been lost, although the information of the properties of Sargassum sp. have been re-examined and collected in Chinese medical books called "Compendium of Materia Medica", written by Shizhen Li in 1578. The most ancient information about Sargassum regards the ability of this algae to treat thyroid related diseases such as goitre, but the Compendium also affirm that Sargassum sp. can soften hard lumps, dispel nodes, eliminate phlegm and induce urination in humans [250].
The information collected in the Compendium can be a valid starting point to evaluate which species of Sargassum were already involved to treat diseases and it provides the opportunity for the development of future research, as the lack of biochemical and pharmacological knowledge that ancient medicine had at the time [251].
Among Sargassum species, the traditional Chinese and Korean medicine adopted the use of Sargassum pallidum, Sargassum confusum to relieve and treat diseases given by goitre, scrofula, swelling and pain of testes, oedema due to retention of phlegm and morbid fluids. These brown algae have been investigated in modern Chinese medicine as well with positive outcomes; in fact, it has been proved that Sargassum pallidum and Sargassum confusum might also be used to treat arteriosclerosis, skin diseases, high blood pressure, hepatosplenomegaly, neurosis, angina pectoris, acute esophagitis and chronic bronchitis [252].
Recent research on those seaweeds suggested that Sargassum sp. may play a role as an immunomodulator, as their bioactive metabolites may inhibit thyroid growth induced by excessive iodine intake and improve immune function, which may be useful in treating Hashimoto's thyroiditis [253]. Sargassum thunbergii and Sargassum horneri have been widely used as popular medicines and food ingredients in the southeast region of China, as well as for treating scrofula, goitre, sore throat, cough and phlegm stasis, angina pectoris, dropsy, dysuria and furuncle, giving the idea to be involved in the modern Chinese medical practice [253].
However, updated information regarding the treatment of thyroid related diseases (e.g., goitre) claims for Sargassum in traditional Chinese medicine has not been sufficiently researched yet. However, with the development of new technologies, it has been possible to prove the beneficial effects of seaweeds on human health. In the last decades, in vitro and in vivo assays have been performed to prove antioxidant, antimicrobial, antiviral and antitumoural properties of seaweed extracts, with particular attention to the isolation and mechanism of action of each compound. Moreover, the use of seaweeds is sustainable and cheap, as they grow in every type of aquatic environment and are easily cultivated depending mainly on sunlight, aeration (natural or artificial) and seawater rich in nutrients. To overcome limitations related to common hydrocolloids extraction, which is time and energy consuming and involves chemical solvents, new extraction methodologies have been investigated. For example, the use of the deep eutectic solvents, as they can be prepared from natural compounds easily available. Smith et al. [254] classified deep eutectic natural solvents in four groups: (a) combination of organic salts and metal salts, (b) combination of organic salts and metal hydrates, (c) mixture of organic salts and compound being hydrogen bond donors and (d) combination of metal chlorides and compound being hydrogen bond donors. Their low costs, biopolymer dissolution ability, biodegradability, non-toxicity, polarity and recyclability make deep eutectic natural solvents a green alternative to replace organic solvents during seaweed's polysaccharide extraction [255]. Indeed, these natural solvents have been successfully used in the extraction of polysaccharides from brown seaweeds [256][257][258][259] and red seaweeds [260], revealing the great potential of deep eutectic solvents in the extraction of seaweed biopolymers for industrial purposes.
The versatility of properties exhibited by seaweeds compounds allows them to be involved in several biotechnological applications and give life to different kind of products.
According to a study from Fortune Business Insights (https://www.fortunebusinessinsights. com/industry-reports/commercial-seaweed-market-100077, accessed on 20 November 2021), the global commercial seaweed market is projected to grow from $15.01 billion in 2021 to $24.92 billion in 2028. Over the past few years, the utilization of hydrocolloids as carrageenan, alginate and agar has been increased in food, pharmaceutical and other industries.
Cargill, Inc. (agricultural products, Minnetonka, MN, USA) launched the Red Seaweed Promise™, a program specifically designed to address key sustainability challenges for the harvesting and cultivation of red seaweed needed to produce carrageenan, for various applications such as dairy, confectionery and personal care products. In the same way, since the 1960s, DowDuPont Inc. (chemicals, Midland, MI, USA) has been the pioneer in making seaweed harvesting as efficient and sustainable as possible, to develop new food sources. Other companies that invested in seaweed production cited in the report of Fortune Business Insights, are: Kerry Group PLC (food corporation, Tralee, Ireland), global leader in the development of taste and nutrition solutions for food, beverage and pharmaceutical markets; Acadian Seaplants (food-supply chain company, Dartmouth, Canada), active since 1981, the first industry to sell extracts of Ascophyllum nodosum and Chondrus crispus as unique seafood in the 1990s; Gelymar Industries (food, personal care and pharmaceutical products, Providencia, Chile), which has extracted hydrocolloids from seaweeds for food, pharmaceutical and personal care since 1990; Groupe Roullier (agriculture products, Saint-Malo, France), a French agribusiness conglomerate founded in 1959 that cultivates seaweeds for the development of animal and plant nutritional products; Corbion Chemicals Company (chemicals, Amsterdam, Netherlands), which released on the market AlgaPrime™ DHA, an algae-based omega-3 feed ingredients for aquaculture, production animals and companion animals; Ocean Harvest Technology Limited (animal feed, County Galway, Ireland), the company responsible for the development of OceanFeed™, an animal feed that helps animals to perform better in a natural way, as the feed ingredients are derived from seaweeds. OceanFeed™ has been also shown to support reduction of antibiotics and other synthetic feed ingredients.
It is important for companies that want to invest in the seaweed economy to adapt to a biology-based management. This means having sustainable approaches, adapting the harvesting and the production based on the life cycle and bioavailability of seaweeds.
Moreover, it is necessary to empower seaweed producers, give them training and specific tools that will improve the yields of seaweeds, and have scientific consultants to detect the biological properties of seaweeds.

Main Conclusions of the Pharmaceutical Potential of Marine Macroalgae and Perspectives
The scope of the present paper was to assess the potential of seaweed as a novel source for marine pharmaceuticals, which aims to develop novel drugs with compounds of natural origin and to diminish collateral effects due to synthetic compounds. Nowadays, the access to essential medicines is a luxury that only less than 50% of the world population has, due to the high price of medicines and the low purchasing power of the population of countries such as Africa and Asia [261].
To overcome health issues, the first line of defence of this population is the traditional medicine that involves the use of natural elements such as plants, herbs and seaweeds to treat diseases and disorders [262,263]. Therefore, the use of traditional medicine can be coupled with the modern medicine, as is happening nowadays in developed countries such as Canada, France and Australia [262]. However, seaweeds used in traditional medicine have a lack of proper biochemical characterization of the extracts, which can lead to dangerous outcomes, for example the insurgence of allergies or the assimilation of toxic compounds that can damage our organisms. There has been a high number of studies conducted about seaweeds' compounds and extracts; nevertheless, further studies should be performed to go further and discover novel molecules to include in several biotechnological applications, thus directly and indirectly improving human welfare.

Conflicts of Interest:
The authors declare no conflict of interest.