Marine Biocompounds for Neuroprotection—A Review

While terrestrial organisms are the primary source of natural products, recent years have witnessed a considerable shift towards marine-sourced biocompounds. They have achieved a great scientific interest due to the plethora of compounds with structural and chemical properties generally not found in terrestrial products, exhibiting significant bioactivity ten times higher than terrestrial-sourced molecules. In addition to the antioxidant, anti-thrombotic, anti-coagulant, anti-inflammatory, anti-proliferative, anti-hypertensive, anti-diabetic, and cardio-protection properties, marine-sourced biocompounds have been investigated for their neuroprotective potential. Thus, this review aims to describe the recent findings regarding the neuroprotective effects of the significant marine-sourced biocompounds.


Introduction
Considering their co-evolution with the associated biological targets, natural products have been favored by scientists for drug discovery and development in the treatment of various human diseases [1,2]. In this context, natural compounds represent the main treatment strategy for 87% of human diseases [3], with 63% of the newly developed drugs being categorized as naturally derived, either modified natural products, unmodified natural products, or synthetic products with a natural compound as the pharmacophore. Moreover, approximately 68% of all anti-infectious drugs, including antibacterial, antiviral, antifungal, and antiparasitic compounds, and 63% of anti-cancer drugs used between 1981 and 2008 were obtained from natural sources [4]. The advancements in the field of natural products are based on their considerable impacts on the pharmaceutical interests and the associated economic activities. Thus, there is a high interest in the discovery of small drug molecules from templates and designs of biologically and chemically diversified natural product pools, the development of novel separation, purification, and characterization techniques and the establishment of test scaffolds [5][6][7]. In this manner, sampling understudied locations of the planet to enhance the knowledge in the biogeography area is fundamental [8].
While terrestrial organisms represent the primary current source for developing natural therapeutics, there has been an increasing interest in focusing on marine organisms [1]. As oceans occupy more than 70% of the earth's surface, their biodiversity is a source of various types of microand macro-organisms offered by different oceanic zones, which makes them an essential reservoir of natural products [4,[9][10][11]. The marine environment, through the use of fish and algae, has represented fishing industry waste, some fungi, mollusks, and nematodes can also be a source [43][44][45]. Chitin is obtained as a colorless or off-white powder that is insoluble in aqueous media or polar solvents due to its high cohesive energy caused by strong intermolecular hydrogen bonds formed between amide bonds [41,44,45]. Chitin is mainly used for producing chitosan, which is the partial or full alkaline deacetylation product of chitin [41,43,[48][49][50]. In this manner, during the deacetylation process in the presence of sodium hydroxide, the acetyl bonds are broken to form glucosamine [43], and a linear polysaccharide with randomly distributed β-(1,4)-linked D-glucosamine and N-acetyl-D-glucosamine is formed [42,45,49,[51][52][53]. Many studies have reported biocompatibility, biodegradability, non-toxicity, immunomodulatory, anti-tumor, antioxidant, hypolipidemic, neuronal regulatory, and anti-microbial properties of chitosan, which highly depend on the degree of deacetylation and the polymer chain size, i.e., an 87 kDa chitosan proved to be more effective than a 532 kDa chitosan against bacterial strains and a lower deacetylation degree leads to a higher degradation rate and host inflammatory response [42,43,48,51,52,54,55]. A wide array of studies investigated the potential of chitosan for neuroprotection. In this manner, chitosan nanoparticles have proved their therapeutic effects on BV-2 glial cells, an immortalized rat microglial line that mimics the characteristics of primary microglia, exposed to hydrogen peroxide [56]. Similar results were obtained using chitooligosaccharides on SH-S5Y5 neurons after exposure to hydrogen peroxide, with the highest activity at the lowest concentration of 0.02 mg/mL [57]. Furthermore, carboxymethylated chitosan protected Schwann cells against hydrogen peroxide-induced damage and apoptosis, resulting in decreased lactate dehydrogenase release and enhanced cell viability through the mitochondrial-dependent pathway [58]. Additionally, similar studies were performed using protocatechuic acid-grafted chitosan and rosmarinic acid-loaded chitosan nanoemulsions on neuron-like rat phaeochromocytoma cells and rat astrocyte primary cultures, respectively. Both studies showed promising neuroprotective effects against hydrogen peroxide and L-glutamic acid-induced apoptosis and LPS-induced oxidative stress [59,60]. Moreover, the neuroprotective effects of chitosan were investigated on PD models. Specifically, low molecular weight sulfated chitosan proved its potential to reduce the consequences of the disease on rotenone-treated SH-SY5Y cells [61]. Similarly, rotigotine-and naringenin-loaded chitosan nanoparticles showed alleviated effects of 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells [62,63]. Additionally, rotigotine administration to haloperidol-induced PD rats led to decreased lactate dehydrogenase and increased catalase activities, as well as catalepsy reversal, akinesia, and swimming ability restoration [63]. Beneficial effects have also been shown against multiple sclerosis, using dimethyl fumarate-loaded chitosan nanoparticles on rodent models that led to significantly increased locomotion scores [64]. Another study proved the repair potential of LINGO-1-directed siRNA-loaded chitosan nanoparticles on demyelinated rat models with compromised motor performance and coordination [65]. Additionally, chitosan scaffolds have been shown to give a high nerve fiber regeneration capacity when compared to alginate or chitosan-alginate scaffolds in spinal cord injury rat models [66].  Marine flora has received increasing interest as a source for marine polysaccharides due to their abundance, unique physicochemical properties, and low extraction costs [67]. Marine algae represent an ideal candidate for extracting polysaccharides owing to their various biological properties, including antioxidant, antiviral, antibacterial, anticancer, anti-inflammatory, immunomodulation, blood coagulation, hypolipidemic and hypocholesterolemic, and osteoprotective effects, which are crucial for pharmaceutical and biomedical research [40,[67][68][69][70]. They cannot be found elsewhere [71]. The essential seaweed-derived polysaccharides with neuroprotective effects are alginate, carrageenan, fucoidan, and laminarin [67][68][69]71].
Alginate, the term generally used for alginic acid salts and derivatives, but also for alginic acid itself, is a natural linear polysaccharide consisting of β-(1,4)-linked D-mannuronic acid and α-(1,4)-linked L-guluronic acid units [72][73][74][75][76]. Consequently, the polymeric backbone consists of homogenous sequences of mannuronic acid (M) or guluronic acid (G) blocks, and alternating sequences (MG) [72,77,78]. Since β-1,4 linkages confer a 4C1 chair conformation that imparts flexibility to the chain and α-1,4 linkages lead to a 1C4 conformation, which is considerably stiff, the stiffness of the backbone blocks decreases in the order GG, MM, and MG [72]. Alginate is extracted from brown algae, where it exhibits structural functions as a cell wall component, comprising about 30% dry weight. Most common sources of alginate are Laminaria hyperborea, Laminaria digitata, Macrocystis pyrifera, Ascophyllum nodosum, and Laminaria japonica [72][73][74][75]77,79,80]. Alginate is usually extracted through the dissolution of seaweed biomass with a basic solution, precipitation in calcium chloride, filtration, purification, and drying steps [75,80]. The physicochemical properties of alginate, such as gel formation or viscosity, are directly influenced by the ratio of mannuronic to guluronic acid, the arrangement and length of the blocks, and the molecular size of the polymers, which differ depending on the isolation source or the extraction season [72,75,77]. One research group investigated the neuroprotective effects of seleno-polymannuronate prepared from alginate-derived polymannuronate. Their results suggested that this biocompound exhibited increased mitochondrial membrane potential and inhibition of amyloid-β aggregation and reduced APP and BACE1 protein and cytochrome c expression in N2a-sw cells, proving its potential in preventing neurodegeneration [81]. Moreover, ellagic acid-loaded calcium-alginate nanoparticles were administered to male Swiss albino mice with pentylenetetrazol-induced seizures. Results showed the superior effects of this system, which prevented increased glutamate, decreased γ-aminobutyric acid concentrations, and ameliorated increased amyloid-β and homocysteine levels [82]. Similarly, erythropoietin-alginate microspheres administered in Balb/c-strain mice improved locomotor and glutathione peroxidase activity, with no significant differences when using increased polymer concentrations [83]. Furthermore, paclitaxel-encapsulated poly(lactic-co-glycolic acid) microspheres embedded in alginate hydrogels provided a sustained drug delivery system in spinal cord injury models [84].
Fucoidan comprises a complex family of natural water-soluble sulfated polysaccharides [69,95,[101][102][103] containing long type I and type II branched chains. Type I chains contain repeating (1,3)-linked α-L-fucopyranose residues, while type II chains contain alternating (1,3)-and (1,4)-linked α-L-fucopyranose residues. Additionally, these compounds also consist of sulfated galactofucans with backbones built of (1,6)-β-D-galacto-and/or (1, 2)-β-D-mannopyranosyl units and other monosaccharides, including uronic acid, xylose, rhamnose, glucose, arabinose, and xylose [69,95,101,102,104]. Fucoidans are mainly isolated from the cell wall and mucous matrix of various species of brown algae, such as mozuku, kombu, limu moui, bladderwrack, and wakame [69,101,102]. Generally, seaweed species, geographic location, and extraction season and procedures directly influence the molecular weight, monosaccharide composition, and sulfate content and position [95,101,105]. Fucoidan has received considerable scientific interest for its neuroprotection activities. In this regard, Ecklonia cava-extracted fucoidan showed significant antioxidant activities on hydrogen peroxide-induced cytotoxicity in PC-12 and MCIXC cells and neuron-protective effects comparable to vitamin C, by regulating mitochondrial function and acetylcholinesterase inhibition [106]. Similarly, the effects induced by the combination of non-invasive low intensity pulsed electric field and fucoidan against hydrogen peroxide-induced neuronal damage were investigated on the motor neuron-like cell line NSC-34, showing improved results and neuroprotective potential [107]. Furthermore, one study proved the protective activity of Sargassum hemiphyllum-extracted fucoidan against 6-hydroxydopamine-induced apoptosis on SH-SY5Y cells [108]. One group investigated the neuroprotective effects of five distinct fucoidan types prepared from Fucus vesiculosus and Undaria pinnatifida against amyloid-β aggregation and cytotoxicity. They demonstrated a wide range of neuroprotective activities that may have the potential to alter amyloid-β neurotoxicity in AD [109]. Additionally, Laminaria japonica-extracted fucoidan was investigated for its protective effects on the dopamine system and mitochondrial function of dopaminergic neurons in the rotenone-induced PD rat model. Results showed a significantly reversed nigral dopaminergic neuron and striatal dopaminergic fiber loss, reduced mitochondrial respiratory function as detected by the mitochondrial oxygen consumption, and ameliorated behavioral deficits [110]. Moreover, fucoidan has proved its potential in the treatment of transient global cerebral ischemia in gerbil models by relieving the acceleration and exacerbation of ischemic brain injury through the attenuation of oxidative damage and glial cell activation [111,112].
Laminarin or laminaran is a biodegradable and non-toxic linear polysaccharide consisting of β-D-glucans linked by (1,3) and (1,6) glycosidic bonds at different ratios. It is extracted from the cell wall of brown seaweeds, such as Laminariaceae, the original source, but also from Laminaria, Saccharina, or Eisenia species [67,95]. Laminarin has been investigated for its neuroprotective effects in the Cornu Ammonis 1 field of the hippocampus, which is highly vulnerable to ischemia-reperfusion injury, following transient forebrain ischemia in gerbils using histopathological samples. While pretreatment with 10 mg/kg failed to protect neurons, 50 or 100 mg/kg proved to be efficient as a preventive strategy against injuries following cerebral ischemic insults by attenuating reactive gliosis and reducing pro-inflammatory microglia [113]. While such a dose is relatively high, further studies that focus on elucidating the mechanisms responsible for the neuroprotective action could lead to a decrease in the necessary dose and an enhanced activity.

Marine Glycosaminoglycans for Neuroprotection
Chemically, glycosaminoglycans are long linear heteropolysaccharides consisting of repeating disaccharide units comprising an amino sugar, either N-acetylgalactosamine or N-acetylglucosamine, and uronic acid, either glucuronate or iduronate [114][115][116]. Additionally, sulfate and hydroxyl groups can be present, imparting a strong negative charge and an extended conformation [114]. Excepting hyaluronic acid, glycosaminoglycan chains form proteoglycans by covalently binding to polypeptides as core proteins [114,117]. Although most commercial glycosaminoglycans are extracted from terrestrial animals, marine glycosaminoglycans have achieved a great scientific interest as they are different in terms of molecular weight and sulfation and, consequently, biological activity [118]. Hyaluronic acid (HA), chondroitin sulfate (CS), and heparin and heparan sulfate (HS) (Table 2, Figure 2) are the most physiologically important glycosaminoglycans involved in neuroprotective activities that can be extracted from marine sources. Table 2. The sources of the main marine-derived glycosaminoglycans.

Marine Sources
Hyaluronic acid marine bivalves (Mytilus galloprovincialis and Amussium pleuronectus), stingray (Aetobatus narinari), mussels, codfish bones, tuna eyeballs, and shark fins HA, also known as hyaluronan, is a natural linear, anionic, non-sulfated polysaccharide produced through the polymerization of D-glucuronic acid and N-acetyl-D-glucosamine linked by alternating glucuronidic β-(1,3) or β-(1,4) bonds, activated by the hyaluronan synthase enzyme [89,116,[119][120][121][122][123][124]. It provides the backbone for specifically binding domains in the aggregating proteoglycan aggrecan [119,122]. HA is a naturally occurring biopolymer with biocompatible, biodegradable, and viscoelastic properties and a molecular weight ranging from 50 kDa to 2 million kDa [89,123]. As the last decade has witnessed a growing interest in isolating HA from marine organisms, several groups have reported the extraction of low molecular weight HA from the marine bivalves Mytilus galloprovincialis and Amussium pleuronectus and high molecular weight HA from stingray (Aetobatus narinari) liver [125]. Other marine sources include mussels, codfish bones, tuna eyeballs, and shark fins [126]. HA has been investigated for its neuroprotective potential in spinal cord injuries. Specifically, a HA/methylcellulose hydrogel was injected into a syringomyelia rat model, showing improved tissue and functional responses and reduced lipopolysaccharide-mediated microglial activation in vitro [127]. Moreover, this type of hydrogel has been modified with an anti-inflammatory peptide and a brain-derived neurotrophic factor, which significantly enhanced the proliferation of PC12 cells and the recovery in both neurological function and nerve tissue morphology in rat models by regulating inflammatory cytokine levels and improving axonal regeneration [128]. Additionally, HA has also proved its efficiency in stroke management, as neuroglobin-loaded sodium hyaluronate nanoparticles have been intravenously introduced in rat models and reached damaged cerebral parenchyma at early stages [129].  [130,133]. Their versatility has led to a broad range of biological activities and various therapeutic, pharmacological, and nutraceutical applications [134]. Moreover, depending on the sources, either terrestrial or marine, CS contains various chain lengths and oversulfated disaccharides at different relative concentrations, i.e., shark, CS-D, dogfish, CS-A and CS-D, squid and salmon, CS-E, and ray, CS-A and CS-C [133]. Specifically, studies have reported the isolation of CS from the blackmouth catshark (Galeus melastomus) [118], corb (Sciaena umbra) skin [135], and various shark and other fish cartilage [136]. Experimental researches have indicated the benefits of CS in the therapy of neurodegenerative diseases [137], with one study reporting the neuroprotective effects of CS against advanced glycation end products-induced toxicity, which has been linked to amyloid-β aggregation, oxidative stress, and inflammation [138]. Moreover, low molecular weight CS and selenium-CS nanoparticles have been shown to protect SH-SY5Y cells by inhibiting amyloid-β aggregation, decreasing reactive oxygen species and malondialdehyde levels, and increasing glutathione peroxidase levels [139,140].
Heparin and HS are the most structurally complex glycosaminoglycans, consisting of identical repeating disaccharide units of hexuronic acid, which can be either β-D-glucuronic acid or its C-5 differential isomer, α-L-iduronic acid, and N-acetylgalactosamine through 1,4 linkages. The 2-O position of the hexuronic acid can be sulfated and the 3-O and 6-O positions of the glucosamine can be replaced by an O-sulfo group. The probability of 6-O substitution is greater than 3-O, and the amino group can be sulfonated, acetylated, or unmodified. Heparin and HS have similar structures, but differ in terms of monomer proportion, i.e., heparin has a large proportion of iduronic acid, while HS mainly consists of glucuronic acid. Additionally, heparin has an average of 2.7 sulfated groups compared to 1 in HS, but a lower molecular weight, 12 kDa compared to 30 kDa. Moreover, while heparin is the most negatively charged macromolecule of the human body and, therefore, the most acidic one, HS is characterized by a higher structural heterogeneity [141,142]. The arrangement of the sulfate groups in short segments of the chains produces binding sites for protein ligands to form proteoglycans through O-ether linkages [143][144][145]. Marine animals are evolutionally secluded from terrestrial mammals, and they represent an important source of heparin and HS extraction, as there is a lower risk of microorganism contamination. Thus, marine mollusks, such as bivalves, gastropods, and cephalopods species, sea cucumbers [146], shrimp heads (Litopenaeus vannamei and Penaeus brasiliensis), clams (Anomalocardia brasiliana, Tivela mactroides, Donax striatus, and Tapes phlippinarum), crabs (Goniopsis cruentata and Ucides cordatus), scallop (Nodipecten nodosus), ascidian (Styela plicata), sand dollar (Mellita quinquisperforata) [147], and cockle (Cerastoderma edule) [148] represent great sources of heparin and HS. One study investigated the effects of heparin, heparinase III, chondroitinase, hyaluronic acid, and an MMP-2/9 inhibitor together with amyloid-β oligomers on cortical and hippocampal populations generated from human-induced pluripotent stem cell-derived neural spheroids. Results showed that heparin administration reduces amyloid-β-related neural cell death [149]. Furthermore, heparin administration in gerbils proved that pre-treatment considerably reduces neuronal cell apoptosis and expression of tumor necrosis factor-α and interleukin-1β, thus exerting neuroprotective effects against cerebral ischemia/reperfusion injury [150]. Additionally, HS is a promising therapeutic strategy to protect and repair the brain after stroke, favoring functional recovery [151].

Marine Glycoproteins for Neuroprotection
Lectins are clusters of oligomeric carbohydrate-binding glycoproteins ubiquitously found in animals, plants, and microorganisms. The "lectin" term is derived from the "legere" Latin word, which means "to select"; precisely, they have a highly specific carbohydrate recognition domain, which offers them the capacity to specifically and reversibly bind to sugar moieties [152][153][154][155][156]. Lectins are a diversified group of proteins of nonimmune origin with many biological roles, including aggregation of animal cells, as they are often referred to as "agglutins", mediation of cell-cell interactions, homeostatic regulation, and immune recognition of foreign carbohydrates [152,154,155]. Depending on their amino acid sequence and biochemical actions, lectins can be classified in several families. Specifically, lectins in fish include C-type lectins, galectins, pentraxins, X-type lectins/intelectins, calnexin, and calreticulin, which can be found in other animals, and F-type lectins, rhamnose-binding lectins, and pufflectins, which have been discovered in fish. They fulfill different biological roles, such as pathogen recognition and opsonization, complement activation, immune function regulation, or they act as antifreeze proteins or prevent polyspermy during fertilization [155]. So far, lectins have been isolated from a variety of marine animals, such as sponges (Aplysina lactuca, Cliona varians, Suberites domuncula, Axinella corrugata, and Chondrilla caribensis), annelids (Cinachyrella apion, Chaetopterus variopedatus and Serpula vermicularis), mollusks (Aplysia dactylomela, Mytilus galloprovincialis, Argopecten irradians, Ruditapes philippinarum, Madiolus modiolus, and Crenomytilus grayanus), arthropods (Tachypleus tridentatus and Penaeus monodon), sea cucumbers (Holothuria scabra, Holothuria grisea, and Cucumaria echinata), ascidians (Didemnum ternatanum), amphioxus (Branchiostoma belcheri and Branchiostoma japonicum), and fish (Aristichtys nobilis, Silurus asotus, Oncorhynchus tshawytscha, Epinephelus coioides, Oncorhynchus mykiss, Rachycentron canadum, and Paralichthys olivaceus) [157,158]. As they have the ability to modulate molecular targets in the central nervous system, lectins might be involved in processes associated with neuroplasticity, neurobehavioral effects, and neuroprotection [159]. Therefore, galectin-3, a β-galactoside-binding lectin, could modulate the innate immunity and induce a therapeutic shift in microglia polarization, significantly reducing the infarct size, in the context of ischemic injury [160]. Similarly, pentraxin-3, an angiogenesis key regulator, has demonstrated its potential and clinical relevance by providing sustained long-term neurovascular repair after stroke and reducing neuronal loss [161].

Marine Lipids and Glycolipids for Neuroprotection
Lipids are responsible for various complex and physiological roles, including cell membrane formation, cell transport, energy storage, signaling, and transmembrane protein modulation. Their composition in the brain depends on age, sex, neuron activity, stress, and trauma, and variations in their concentration, organization, and metabolism might consequently lead to neurological and/or mental disorders [162]. In the brain, the most abundantly found organic compounds are polyunsaturated fatty acids, which are further classified into ω-6 and ω-3 polyunsaturated fatty acids, derived from linoleic acid and α-linolenic acid, respectively [162,163]. While they were generally ignored for more than 40 years, polyunsaturated fatty acids are essential for normal brain development and function [162][163][164][165][166]. ω-3 fatty acids play fundamentally important biological roles, including neurotransmission, signal transduction, receptor binding, and eicosanoid synthesis, and aid in synaptic plasticity and neuroprotection [165,166]. A lack of ω-3 fatty acids has been linked to a chronic pro-inflammatory state in the brain that further leads to dementia and an increased risk of cerebral ischemia [167].
Examples of fatty acids include docosahexaenoic acid, eicosapentaenoic acid, α-linolenic acid, arachidonic acid, linolenic acid, and oleic acid (Table 3, Figure 3) [168]. Fish oils, especially cod liver oil from Atlantic cod (Gadus Morrhua L.), are the main source of ω-3 fatty acids [169]. Additionally, microalgae are emerging as a new source for extraction in order to sustain the needs of the population. Due to their increased bioactivity, docosahexaenoic acid, and eicosapentaenoic acid are the most nutritionally significant fatty acids produced [170]. Table 3. The sources of the main polyunsaturated fatty acids obtained from marine sources.  As some studies reported the beneficial effects of some polyunsaturated fatty acids in AD by reducing amyloid-β toxicity through enhancing its degradation and clearance, one group investigated the interactions of fatty acids docosahexaenoic acid, eicosatetraenoic acid, α-linolenic acid, arachidonic acid, linoleic acid, and oleic acid with amyloid-β peptides. Results showed that all the fatty acids tested have anti-aggregation properties by preventing amyloid-β40 and amyloid-β42 fibrillogenesis, thus providing a novel direction for developing a therapy for AD [171]. Furthermore, docosahexaenoic acid has been shown to play a crucial role in neurogenesis, antinociceptive effects, anti -apoptotic effects, synaptic plasticity, Ca 2+ homeostasis in brain diseases, and nigrostriatal activity functioning, with a high intake of docosahexaenoic acid-containing foods being linked with a lower risk of AD and other brain disorders [172]. In this regard, one study showed that the administration of docosahexaenoic acid-enriched phosphatidylcholine and docosahexaenoic acid-enriched phosphatidylserine in AD SAMP8 mice models improved the metabolic disorders and cognitive deficits by ameliorating amyloid-β pathology, mitochondrial damage, neuroinflammation, and neurotrophic factors and oxidative stress, respectively. The molecular mechanisms responsible have been found to be closely related to the phospholipid polar groups [173]. Moreover, the effects of docosahexaenoic acid and eicosapentaenoic acid, either alone or in combinations of 1:1, 1:2 and 2:1, as found in sea seals, sea algae, and fish oil, on cellular models of AD have been investigated. Results demonstrated that both fatty acids attenuate neuron apoptosis and improve cell viability with synergistic anti-inflammatory effects in the AD model; additionally, pure eicosapentaenoic acid is more effective against oxidative stress, while pure docosahexaenoic acid better improves neurotrophic systems [174]. Furthermore, eicosapentaenoic acid-enriched phospholipids extracted from the sea cucumber (Cucumaria frondosa) improved MPTP-induced PD in mice by suppressing oxidative stress and apoptosis and alleviating the loss of dopaminergic neurons via mitochondria-mediated and mitogen-activated protein kinase pathways [175].

Biocompound
Glycolipids comprise a large class of natural compounds consisting of a glycosidic fragment linked to a lipid molecule. Although highly structurally variable, glycolipids can be classified into three main categories, namely glycosphingolipids, glycoglycerolipids, and atypical glycolipids [176].
Chemically, glycosphingolipids comprise sphingosine and fatty acid residues, which are linked to an amide in the ceramide and contain no phosphate groups. Furthermore, a carbohydrate links through a β-glycosidic bond to the primary alcohol oxygen atom of the ceramide [177]. Glycosphingolipids represent the building blocks of the outer leaflet of the cell membrane in a wide variety of terrestrial and marine organisms, where they play fundamental physiological roles due to variations in the sugar chains. They are continuously recycled inside lysosomes by glycosidase fragmentation [178,179]. Based on the constituent sugars, glycosphingolipids can be further classified into cerebrosides, ceramide oligohexosides, globosides, and gangliosides. Echinoderms, porifera, and mollusks have been identified as suitable marine sources for glycosphingolipid isolation [179]. One study investigated the protective effects of sea cucumber-derived cerebrosides against amyloid-β-induced cognitive impairment on AD male rat models. Results proved the neuroprotective capacity of the marine-derived glycolipids by ameliorating neuronal damage and suppressing the induced apoptosis [180]. Moreover, gangliosides, a family of glycosphingolipids containing sialic acid linked to an oligoglycosyl backbone, which is further attached to a ceramide base, are highly expressed in vertebrate nervous systems [181]. Additionally, they are widely present in marine echinoderms, such as sea cucumbers, sea urchins, and starfishes, which are found to be different from mammalian glycosphingolipids in terms of their basic sugar moiety and the types and numbers of sialic acids (i.e., mammalian glycosphingolipids contain mainly N-acetyl-neuraminic acid, while echinoderm glycosphingolipids contain N-acetyl-neuraminic acid, N-glycolylneuraminic acid, and sulfated N-glycolylneuraminic acid). One study reported the neuroprotective effects of the sea urchin Strongylocentrotus nudus-isolated glycosphingolipids on AD models of Ab25-35-induced PC12 cells and SAMP8 mice as in vitro and in vivo models. The main mechanisms involve inhibition of synaptic loss through synaptophysin and GAP-43 expression and mediation of mitochondrial apoptosis, which is directly related to the neurofibrillary pathology [182].
Marine microbial-derived glycolipids have been intensively studied since they are widely produced by a broad spectrum of bacteria extracted from different marine matrices, including animals, e.g., Annelida, Pteroides spinosum, or fish gut, and contaminated soils [184]. Furthermore, microalgae have become a promising lipid source due to their lipid accumulation mechanisms triggered by various stress conditions, such as limited nutrients or damaging physical factors [185]. Generally, they produce a great variety of lipids, including polar lipids, neutral lipids, wax esters, hydrocarbons, or sterols [186].

Marine Pigments for Neuroprotection
Pigments are molecular structures capable of absorbing specific wavelengths of light and reflecting the rest of the visible spectrum. Moreover, microbial pigments possess additional chemical component mixtures that have complex biological activities, such as antimicrobial, anticancer, and immunomodulation. Thus, recent years have witnessed a tremendous increase in the study of terrestrial and marine microbial pigments [187][188][189][190][191]. The production of pigments by marine bacteria is presumably mediated by the quorum-sensing mechanism [188]. Most common types of pigment compounds from marine microorganisms include carotenoids, polyunsaturated hydrocarbons with 30, 40, or 50 carbon atoms in one molecule, melanins, polyphenolic pigments obtained through hydroxylation, oxidation, and polymerization reactions of phenolic compounds, phenazines, tricyclic, redox-active, and small nitrogen-containing heterocyclic aromatic compounds, prodiginines, aromatic chemical compounds with pyrrolyl dipyrromethene core structures, quinones, compounds containing aromatic ring structures with yellow-to-red color hues, tambjamines, alkaloid compounds with yellow color hues, and violacein, indole-pigmented compounds derived from the metabolism of tryptophan [188].
On the one hand, lycopene is a biocompound that has been widely researched owing to its beneficial effects in the central nervous system. One study proved its potential to attenuate oxidative stress and reduce tert-butyl hydroperoxide-induced cell apoptosis as key factors in the pathogenesis of AD. Lycopene administration led to improved cell viability and neuron morphology, increased GSH/GSSG levels, restored mitochondrial membrane potential, and decreased reactive oxygen species [195]. Similarly, intragastric pretreatment resulted in reduced inflammatory cytokine levels and reversed amyloid-β-induced up-regulation of TLR4 and NF-κB p65 mRNA and protein expressions at the choroid plexus, thereby diminishing amyloid-β deposition in the hippocampus [196]. Additionally, the administration of lycopene has led to alleviated cognition impairment and oxidative stress by decreasing malondialdehyde and 8-hydroxy-2 -deoxyguanosine levels and increasing glutathione level and superoxide dismutase activity in aluminum chloride-induced hippocampal lesions in rat models. These mechanisms have proved to subsequently prevent neuroinflammation and apoptosis [197]. Furthermore, lycopene exhibited neuroprotective effects in MPTP-treated PD mice models by increasing dopamine levels and decreasing oxidative stress levels [198]. Lycopene has also proved to be useful in spinal cord ischemia/reperfusion injury rat models, by improving neurological function recovery and suppressing neuronal death and neuroinflammation [199] and hyperlipidemia-induced cerebral vessel injury prevention by decreasing astrocytes activation and inflammatory cytokine production [200].
On the other hand, astaxanthin, a xanthophyll carotenoid compound, has proved its neuroprotective potential through the inhibition of lipopolysaccharide-induced neuroinflammation, amyloidogenesis, and oxidant activity in mice models [201] and the prevention of hippocampal insulin resistance and AD complications in Wistar rats [202] and brain damage in offspring exposed to prenatal epilepsy seizures [203]. Additionally, astaxanthin and fucoxanthin have also been investigated for their neuroprotective potential against amyloid-β-mediated toxicity in pheochromocytoma neuronal cells. Results demonstrated multi-neuroprotective effects but suggested a higher potential of fucoxanthin as a potential therapeutic strategy [204]. Crocin has also been administered for the therapy of AD and PD, with results proving the potential to treat neurodegeneration [205][206][207]. Additionally, the potential of β-carotene for the treatment of acute spinal cord injury has been investigated, and results showed a reduced progression of secondary injury events through the prevention of the nuclear factor-κB pathway [208].

Conclusions
As natural products are preferred for the discovery and development of drug molecules for the treatment of various human diseases, there have been considerable advancements in the pharmaceutical biocompound industry. Although terrestrial organisms are currently the main source, the marine environment has received significant scientific interest due to its biodiversity, abundancy, and the biological potential of the derived biocompounds. Additionally, their structural and chemical properties are not generally found in terrestrial products, exhibiting considerable bioactivity ten times higher than terrestrial-sourced molecules. Marine biocompounds are of animal, plant, and microorganism origin, each type providing a plethora of compounds, including carbohydrates, polyphenols, peptides, proteins, pigments, and essential fatty acids, that exhibit antioxidant, anti-thrombotic, anti-coagulant, anti-inflammatory, anti-proliferative, anti-hypertensive, anti-diabetic, and cardio-protection properties. Moreover, marine biocompounds have proved their neuroprotective effects through various research studies, mainly aiming at the prevention of neurodegeneration and the reduction of oxidative stress in the central nervous system. However, the field of marine-sourced neuroprotective compounds is still in its infancy, requiring further discoveries and investigations.