Metabolites from Marine Microorganisms, Micro, and Macroalgae: Immense Scope for Pharmacology

Marine organisms produce a large array of natural products with relevance in drug discovery. These compounds have biological activities such as antioxidant, antibacterial, antitumor, antivirus, anticoagulant, anti-inflammatory, antihypertensive, antidiabetic, and so forth. Consequently, several of the metabolites have made it to the advanced stages of clinical trials, and a few of them are commercially available. In this review, novel information on natural products isolated from marine microorganisms, microalgae, and macroalgae are presented. Given due research impetus, these marine metabolites might emerge as a new wave of promising drugs.


Introduction
Oceans cover about 70% of the earth's surface, serving as the habitat of a great diversity of organisms [1]. These organisms produce numerous metabolic products. Especially, lower organisms elaborate a multitude of secondary metabolites as signaling molecules for "defense and offense". These compounds, which belong to diverse chemical classes, can act as potential therapeutics for healthcare [2]. In the past decades, several promising therapeutics have been extracted from bacteria, fungi, corals, micro-and macroalgae, gorgonians, sponges, nudibranchs, bryozoans, sea cucumbers, tunicates, and sea hares, among other marine organisms [3]. Considerable efforts have been directed towards the isolation of these compounds, and at the moment, more than 10,000 natural products (NPs) of potential biotechnological interest have been isolated [4]. The present review reports on the most promising bioactive compounds of marine origin, emphasizing their pharmaceutical potential.

Bioactive NPs from Marine Bacteria and Fungi
In the marine environment, bacteria and fungi are pervasive. In the past decades, the number of reported bioactive compounds derived from marine bacteria and fungi has steadily increased [5]. Marine bacteria produce a large repertoire of secondary metabolites to survive in the hostile oceanic conditions. Among others, thermophilic and archaea bacteria elaborate thermostable enzymes which belong to diverse classes [1,6].

Anticancer Activity
Besides antibiotics, marine microorganisms are also a source of anticancer principles. The bacterium Micromonospora sp. produces thiocoraline ( Figure 1G), a depsipeptide which inhibits cellular DNA polymerase-α. This substance has been applied for the treatment of cancer in preclinical research [14]. An unidentified fungus of the Pleosporales order (strain CRIF2) produces several compounds showing weak cytotoxic activity against tumor cell lines [15]. A Pestalotiopsis sp. fungus, isolated from the leaves of Rhizophora mucronata, produces a chromone, namely, pestalotiopsone F ( Figure 1H), which displays cytotoxic activity against L5178Y murine cancer cells [16].    [20] Inhibits proteasome activity by covalently modifying the threonine residue of the active site of the 20S proteasome   [20] Inhibits proteasome activity by covalently modifying the threonine residue of the active site of the 20S proteasome

Metabolites with Potential Beneficial Activities from Marine Algae
Marine algae are the primary producers of oxygen in the aquatic environment and sit at the bottom of the marine food chain, serving all other organisms [31]. Marine algae can be divided into two main groups: macroalgae (seaweeds) and microalgae, both being prolific sources of bioactive substances [32]. Therefore, research is in progress for evaluating their medicinal prospects.

Marine Microalgae: Blue-Green Algae (Cyanobacteria)
Marine microalgae typically constitute the phytoplanktons. They can be categorized into three groups: blue-green algae (Cyanobacteria), diatoms (Bacillariophyta), and dinoflagellates (Dinophyceae). There are over 50,000 different species of microalgae, of which only a few have been characterized [18]. Significant biochemical differences have been found among marine microalgae, resulting in a broad spectrum of novel bioactive compounds [33] of pharmaceutical interest [34]. Some of them show high antiviral and anti-HIV activity [35,36]. Recently, a new natural anti-AIDS drug has been derived from Lyngbya lagerhaimanii and Phormidium tenue ( Table 2). Calcium spirulan isolated from Spirulina platensis possesses strong antiviral activity [37]. Some cyanobacteria strains produce antifouling compounds with antibiotic activity [38]. For example, the extracts of Lyngbya majuscule have been tested as a potential source of antifouling agents [39]. Some of the cyanobacteria-derived products have multiple properties. For example, ulithiacyclamide and patellamides A and C are known for their antimalarial, antitumor, and MDR-reversing activities [40]. Two new bioactive compounds, dolastatin 13 ( Figure 3C) and lyngbyastatins 5-7 ( Figure 3D), were isolated from Lyngbya spp., which inhibited elastase from porcine pancreas, with an IC 50 = 3-10 nM [41]. Of the three abovementioned groups, cyanobacteria have been credited with the most bioactive compounds. Cyanobacteria (Cyanophyta) are a group of Gram-negative bacteria and one of the richest sources of novel bioactive compounds with antifungal, anti-inflammatory, antibiotic, and antitumor activities (Table 1), which make them interesting candidates for the production of molecules for new potential pharmaceutical applications [42].

Antitumor Activity
Antitumor compounds affecting cell signaling by the activation of the protein kinase C cascade have been demonstrated in cyanobacteria [49]. Cyanobacteria elaborate anticancer compounds, such as dolastatin 10 ( Figure 2D), curacin A, and cryptophycin (Figure 2A), which target tubulin or actin filaments of eukaryotic cells. Dolastatin 10, a strong microtubule inhibitor that can arrest cell mitotic division, was isolated from Symploca sp. [50]. Curacin A was isolated from L. majuscule and is a strong antiproliferative agent, inhibiting microtubule assembly [51]. Cryptophycin was isolated from marine Nostoc sp. GSV 224 and is an anticancer drug candidate with efficacy against L1210 leukemia cells, ovarian carcinoma cells, and drug-resistant breast cancer cells [52]. The mechanism of action of cryptophycin involves binding at the microtubule ends, leading to the disruption of cell mitosis [53]. Odoamide is a newly discovered cyclic depsipeptide from Okeanis sp., showing strong cytotoxicity against HeLa S3 human cervical cancer cells (IC50 = 26.3 nM) [54]. Hierridin B from Cyanobium sp. LEGE 06113 exerted cytotoxicity towards HT-29 colon adenocarcinoma cells [55].

Antitumor Activity
Antitumor compounds affecting cell signaling by the activation of the protein kinase C cascade have been demonstrated in cyanobacteria [49]. Cyanobacteria elaborate anticancer compounds, such as dolastatin 10 ( Figure 2D), curacin A, and cryptophycin ( Figure 2A), which target tubulin or actin filaments of eukaryotic cells. Dolastatin 10, a strong microtubule inhibitor that can arrest cell mitotic division, was isolated from Symploca sp. [50]. Curacin A was isolated from L. majuscule and is a strong antiproliferative agent, inhibiting microtubule assembly [51]. Cryptophycin was isolated from marine Nostoc sp. GSV 224 and is an anticancer drug candidate with efficacy against L1210 leukemia cells, ovarian carcinoma cells, and drug-resistant breast cancer cells [52]. The mechanism of action of cryptophycin involves binding at the microtubule ends, leading to the disruption of cell mitosis [53]. Odoamide is a newly discovered cyclic depsipeptide from Okeanis sp., showing strong cytotoxicity against HeLa S3 human cervical cancer cells (IC 50 = 26.3 nM) [54]. Hierridin B from Cyanobium sp. LEGE 06113 exerted cytotoxicity towards HT-29 colon adenocarcinoma cells [55].

Antimalarial Activity
Antimalarial activity of natural products isolated from cyanobacteria has been reported. Gademann and Kobylinska (2009) isolated an acyl proline derivative, tumonoic acid I, from Blennothrix cantharidosmum, which exhibited moderate toxic activity against Plasmodium falciparum (IC50 = 2 µM) [60]. Two new antimalarial depsipeptides, companeramides A and B, have been extracted from a marine Panamanian cyanobacteria assemblage [61]. Oscillatoria nigro-viridis produces two new linear peptides, viridamides A and B, with antitrypanosomal and antileishmanial activity [62]. Moreover, cyanobacteria are sources of vitamins B and E [63]. Pigments extracted from cyanobacteria, such as carotenoids and phycobiliproteins, are already industrially applied as food coloring additives, as supplements for health and fertility of dairy cattle, and in the cosmetics industry.

Antimalarial Activity
Antimalarial activity of natural products isolated from cyanobacteria has been reported. Gademann and Kobylinska (2009) isolated an acyl proline derivative, tumonoic acid I, from Blennothrix cantharidosmum, which exhibited moderate toxic activity against Plasmodium falciparum (IC 50 = 2 µM) [60]. Two new antimalarial depsipeptides, companeramides A and B, have been extracted from a marine Panamanian cyanobacteria assemblage [61]. Oscillatoria nigro-viridis produces two new linear peptides, viridamides A and B, with antitrypanosomal and antileishmanial activity [62]. Moreover, cyanobacteria are sources of vitamins B and E [63]. Pigments extracted from cyanobacteria, such as carotenoids and phycobiliproteins, are already industrially applied as food coloring additives, as supplements for health and fertility of dairy cattle, and in the cosmetics industry.

Anti-inflammatory Activity
Cyanobacteria metabolites have also shown pronounced anti-inflammatory effects. For instance, bis-bromoindoles from Rivularia sp. displayed powerful anti-inflammatory activity [8]. An antiinflammatory compound malyngamide F acetate has been derived from L. majuscule. This substance inhibited the production of nitric oxide (NO) in stimulated RAW 264.9 cells [64] by blocking the MyD88 inflammation pathway.

Marine Macroalgae
Macroalgae, or seaweeds, are found in intertidal regions and tropical waters. They are multicellular organisms with various arrays of morphological types and sizes and can be further classified by their photosynthetic pigments into red algae (Rhodophyceae), green algae (Chlorophyceae), and brown algae (Phaeophyceae) [65]. Currently, over 3200 novel products have been extracted from macroalgae, the majority of which come from subtropical and tropical waters [66]. Compounds with medical applications, such as antitumor, antioxidant, antiviral, antifouling, anticoagulant, antibacterial, antifungal, and anthelminthic activities, have been detected in macroalgae [67,68]. Red seaweeds are proposed as anticoagulants, anthelmintic, and in the treatments of gastritis and diarrhea [69]. The traditional medical uses of green seaweed spans form anthelmintic to astringent and anti-gout. Brown seaweeds are applied to cure rheumatic diseases, hypertension, arteriosclerosis, menstrual disorders, skin diseases, gastric ulcers, goiter, and syphilis and are also used as anticoagulants. Polysaccharides, such as ulvans from green seaweeds, alginates, fucans, laminarin from brown seaweeds, and carrageenans and porphyrans from red seaweeds, can stimulate defense responses against plant pathogens [70]. Thus, marine algae yield a large diversity of bioactive metabolites and appear to be a potential resource of interesting pharmacological substances. The sections below present the therapeutic compounds and functions of members from each of the three groups of seaweeds.   [71] Stimulates the production of nitric oxide by inducing iNOS at mRNA and protein levels and induces the expression of several cytokine mRNA, such as IL-1β, IL-6, IL-10, and TNF-α   [71] Stimulates the production of nitric oxide by inducing iNOS at mRNA and protein levels and induces the expression of several cytokine mRNA, such as IL-1β, IL-6, IL-10, and TNF-α   [71] Stimulates the production of nitric oxide by inducing iNOS at mRNA and protein levels and induces the expression of several cytokine mRNA, such as IL-1β, IL-6, IL-10, and TNF-α   [71] Stimulates the production of nitric oxide by inducing iNOS at mRNA and protein levels and induces the expression of several cytokine mRNA, such as IL-1β, IL-6, IL-10, and TNF-α

Red Seaweeds
Red seaweeds are commonly considered beneficial for human health and an important source of bioactive compounds [86]. For centuries, their extracts have been applied for the cure of asthma, thyroid goiter, urinary infections, stomach ulcers, and even tumors. Among the compounds isolated from red seaweeds, sulfated polysaccharides are economically the most important bioactive compound because of their wide application in medicine. These polysaccharides are carrageenan, agar ( Figure 4A), agarose, and furcellaran (Danish agar). Carrageenan is produced by the genera Chondrus, Eucheuma, Gigartina, and Iridea and is considered an effective remedy for gastric and duodenal ulcers [87]. Chondrus crispus is a good source of carrageenan that has an antiviral property, in particular against influenza B and mumps virus [88]. Agar and agarose are used for interferon production, usage as antiviral compounds, and improving B-and T-cell activity [89]. A polysaccharide (Mw = 100-500 kDa) isolated from the fermented red seaweed Lomentaria catenata possesses anticoagulant activity [90]. Fucoidan ( Figure 4F), extracted from Gracilaria corticata, showed activity against both colorectal and breast cancer [91]. An anthelmintic compound, L-α-kainic acid ( Figure 4B), has been isolated from Digenea simplex [92]. Deepa et al. (2017) reported on the possible effects of G. corticata on cancer treatments, inflammation, and infectious diseases [91]. Some red seaweeds, such as Rhodomela confervoides, Symphyocladia latiuscula, and Polysiphonia urceolata, produce phenolic compounds, which have shown antidiabetic activity. These compounds possess the capacity to inhibit protein tyrosine phosphatase (PTPase), which is responsible for the response to insulin. Collins reported on the antiasthmatic activity of polyphenolic extracts of Laurencia undulate [68]. A new potent inhibitor of lipoxygenase (LOX), which plays a crucial role in neurodegeneration, has been isolated from Odonthalia corymbifera [93]. The substance is pheophytin A, which can be applied as a new therapeutic, and is considered an excellent opportunity for the treatment of neuropathologies such as Alzheimer's disease.  [95]. An antiviral compound, sulfated xylomannan, has been extracted from the Indian red seaweed Scinaia hatei that inhibited HSV-1 and HSV-2 (IC50 = 0.5-1.4 µg/mL) [96]. Water-soluble polysaccharidic extracts of Sphaerococcus coronopifolius and Boergeseniella thuyoides collected from the coast of Morocco showed antiviral properties against viruses, including HIV and HSV-1 [97]. Serkedjieva (2004) extracted a bioactive compound from Ceramium rubrum isolated from the Black Sea. This metabolite inhibited types A and B influenza viruses, both in vivo and in vitro, followed by the reduction of cytopathogenic effects [98].  [95]. An antiviral compound, sulfated xylomannan, has been extracted from the Indian red seaweed Scinaia hatei that inhibited HSV-1 and HSV-2 (IC 50 = 0.5-1.4 µg/mL) [96]. Water-soluble polysaccharidic extracts of Sphaerococcus coronopifolius and Boergeseniella thuyoides collected from the coast of Morocco showed antiviral properties against viruses, including HIV and HSV-1 [97]. Serkedjieva (2004) extracted a bioactive compound from Ceramium rubrum isolated from the Black Sea. This metabolite inhibited types A and B influenza viruses, both in vivo and in vitro, followed by the reduction of cytopathogenic effects [98].

Antioxidant Activity
Antioxidant properties has been found in several species of red seaweeds, including Gracilaria, Halymenia, Laurencia, Ahnfeltiopsis, and Polysiphonia. For example, mycosporine-like amino acids isolated from Ahnfeltiopsis devoniensis show antioxidant activity [99]. Moreover, two classes of natural compounds, polyphenols and bromophenols, with known antioxidant activity were isolated from red seaweeds [100]. The ethanolic [101] and methanolic [102] extracts isolated from Gracilaria tenuistipitata showed antiproliferative activity on the oral carcinoma cell line Ca 9-22 by modulating oxidative-stress-induced cell apoptosis.

Antibiotic Activity
Rahelivao reported on the properties of crude extracts of the red algae Laurencia complanata, which displayed antibacterial activity against Streptococcus pneumoniae, Bacillus cereus, and S. aureus [81]. Four tetracyclic brominated 1, 4-diterpenes were isolated from the extract of S. coronopifolius, collected from the rocky coasts of Corfu Island (Greece). These diterpenes showed antibiotic activity against a panel of bacteria, including methicillin-resistant S. aureus (MRSA) and MDR strains, with MIC (Minimum Inhibitory Concentrations) values in the 16-128 µg/mL range [103]. Crude methanolic extracts isolated from Acanthaphora spicifera showed antibacterial activity against E. coli, Bacillus subtilis, Pseudomonas aeruginosa, and Bacillus palmitus [104]. Also, antifungal activity of the extracts against C. albicans, Aspergillus niger, and Microsporum gypseum was observed [105].

Brown Seaweeds
Brown seaweeds contain several pigments, such as fucoxanthin, violaxanthin, and β-carotene [112]. Fucoxanthin occurs in edible marine brown algae, including Undaria pinnatifida, Laminaria japonica, Sargassum fulvellum, and Hijikia fusiformis, and possesses antioxidant, anticancer, antiobesity, antidiabetic, and antiphotoaging activities [113]. The ethanolic extract of Turbinaria conoides demonstrated antioxidant, antibacterial, and anticancer properties. The sources and properties of some bioactive principles in the extracts of brown seaweeds are reported in Table 2. For example, organic solvent extracts from Sargassum wightii and Sargassum ilicifolium exhibited an interesting anticancer activity on several cells lines [114]. Several studies on U. pinnatifida demonstrated anti-hyperglycemic, antitumor, anti-hypertensive, and antiobesity potential [115]. Khan reported on an omega-3 essential fatty acid, stearidonic acid, isolated from U. pinnatifida which showed activity against erythema, mouse ear inflammation, edema, and blood flows (IC 50 = 160, 314, and 235 µg/per ear, respectively) [116]. Laminarin is a water-soluble polysaccharide found in brown algae that has strong heparin-like activity and, therefore, is useful as an anticoagulant, antilipemic, antiviral, or anti-inflammatory agent [17]. Park et al. (2011) reported that that fucoidan reduces lipid accumulation by stimulating lipolysis, and it can be beneficial for obesity therapy [117]. Spavieri reported on the antimycobacterial, antiprotozoal, and cytotoxic activity of 21 brown algae (Phaeophyceae) from British and Irish waters [118]. Several other bioactive compounds were reported for fucoidans isolated from different species of brown algae, including antiviral and antibacterial activities [119]. Moreover, fucoidans isolated from brown seaweed species present immunomodulating activity, involving the increased generation of macrophage-mediated responses such as interleukin-2 (IL-2), interleukin-12 (IL-12), and interferon gamma (IFN-γ) [120]. Salgado reported on the interactions between polyphenolic compounds from the brown alga Padina gymnospora and cell wall alginates, leading to the absorption of ultraviolet radiation [121]. Also, diekol isolated from Ecklonia cava showed antifungal, anti-inflammatory, and anti-type-II diabetes activities in mouse models [122].

Antibacterial and Antioxidant Activities
The essential oil derived from U. pinnatifida possesses potent antibacterial and antioxidant activities [114]. The methanolic extracts of Sargassum platycarpum A and Sargassum latifolium B were shown to be highly effective against Gram-positive bacteria [123]. Three novel products from Ecklonia maxima-eckol, dibenzo-dioxine-2,4,7,9-tetraol, and phloroglucinol-were shown to exhibit free-radical scavenging activity by a DPPH assay, and the EC 50 values were 0.008, 0.012, and 0.128 µM, respectively [124]. The phenolic extracts of Sargassum showed antibacterial and antioxidant properties [125].

Antidepressant Activity
Extracts of Sargassum swartzii and Stoechospermum marginatum showed significant stimulant and anxiolytic effects on a rat model, which occurred by the amelioration of brain norepinephrine levels [126]. The aqueous extracts of Cystoseira usneoides, Cystoseira tamarascifolia, Cystoseira nodicaulis, Stypocaulon scoparium, and Fucus spiralis showed antidepressant activity by inhibiting the monoamine oxidase A (MAO-A) enzyme [127].

Anticancer Activity
A cytotoxic metabolite, stypoldione, isolated from Stypodium zonale inhibited microtubule polymerization, thereby preventing mitotic spindle formation in cell cultures [128]. Ethanolic extracts of U. pinnatifida sporophylls induced nonoxidative apoptotic damage on human colon or rectum cancer cells [129]. The derivative of atomaric acid, stypoquinonic acid ( Figure 4C), isolated from S. zonale is an inhibitor of the tyrosine kinase enzyme. Another atomaric acid derivative, dimethoxy-atomaric acid, showed cytotoxic activity against lung and colon cancer cells [130]. Moreover, fucoidan is known to possess other bioactive properties, such as inhibitory effects on the growth of cancer cells [131]. Sodium alginate ( Figure 4J) obtained from brown seaweeds has shown antitumor and anti-inflammatory properties [132]. The anticancer activity was evaluated by analyzing the effects on the cell cycle and apoptosis induction on HepG2 cells [133]. Furthermore, several studies validated the apoptosis induction ability of fucoidans in various tumor cell lines, including melanoma cells, colon cancer, lymphoma, and breast cancer cells [134].

Antiangiogenic and Anticoagulant Activities
Phlorofucofuroeckol A ( Figure 4I), a phlorotannin ( Figure 4H) from Ecklonia kurome, exhibited inhibitory activity on the angiotensin-converting enzyme, with an IC 50 value of 12.74 µM [135]. The sulfated polysaccharides known as fucoidans caused the prevention of cell proliferation and migration and also vascular network formation on human umbilical vein endothelial cells (HUVEC), suggesting significant antiangiogenic activity [136]. Remarkably, this effect deteriorated upon the reduction of fucoidan molecular weight (<30 kDa) [137]. An analogous observation was reported for the anticoagulant activities of fucoidans [138]. A fucoidan isolated from Fucus vesiculosus showed antithrombotic activity [139].
Of course, the clinical application of all these substances depends on further studies and safety evaluations.

Green Seaweeds
Green seaweeds, or chlorophyta, are a large group of macroalgae with worldwide distribution. The morphology of some species is presented in Figure 3. Green seaweeds are highly considered for their production of antioxidants, vitamins, and bioactive peptides [148]. Among their bioactive constituents, cell wall polysaccharides, constituting about 38-54% of the seaweed dry matter, show pharmaceutical potential [149]. Some green seaweeds, such as Caulerpa taxifolia, Caulerpa racemose, and Cladophora pinnulata, show hypotensive activities [111]. At the moment, most of the literature on green seaweed products is focused on sulfated polysaccharides because of their interesting properties, including anticoagulant, antioxidant, anticancer, anti-hyperlipidemic, and immunomodulation effects [150]. Ulvan, a sulfated polysaccharide form Ulva pertusa, has valuable antioxidant activity [151]. It also acts on the plasma levels of low-density lipoprotein (LDL), high-density lipoprotein (HDL), and triglycerides in mice and can be useful for ischemic, cerebrovascular, and cardiovascular diseases [152]. Sulfated polysaccharides from U. pertusa, Capsosiphon fulvescens, and Codium fragile possess potent immune-modulating activity by stimulating macrophages [153]. Also, the ethanolic extracts of Codium tomentosum showed antigenotoxic and antioxidant effects [154] and Codium decorticatum showed antibacterial activity [155]. Moreover, methanolic extracts of Ulva linza, due to their high polyunsaturated fatty acids (PUFA) content, showed high inhibitory activity against inflammatory response [156].

Antioxidant and Anticancer Activities
According to Wang, extracts and monounsaturated fatty acids (MUFA) derivatives from Ulva lactuca induced an antioxidant response in cells [159]. Beta-carotene is a potent antioxidant found in green seaweeds and can be accumulated in very high amounts in Dunaliella salina [160]. Carotenoids, for example, lutein ( Figure 4E) and zeaxanthin from the green seaweed Chlorococcum humicola, exhibited antimutagenic activity against benzo[a]pyrene-induced mutations in histidine-revertant cells of Salmonella typhimurium and were proposed for the reduction of the breast cancer risks [161]. Another carotenoid from green seaweeds, siphonaxanthin ( Figure 4D), effectively induced apoptosis in human leukemia (HL-60) cells by caspase-3 activation, accompanied by the modification of growth arrest and DNA-damage-inducible protein (GADD45α), tumor necrosis factor receptors (DR5/TRAIL receptor-2), and Bcl-2 regulatory protein expression pattern [162]. Ganesan reported that siphonaxanthin derived from C. fragile possesses considerable antiangiogenic activity [163]. Moreover, the ethanolic extracts of C. tomentosum showed antigenotoxic and antioxidant effects [154].

Antibacterial and Antifouling Activities
Extracts of U. fasciata isolated from the southeast coast of India displayed antibacterial properties and a broad spectrum of antibiotic activity against B. cereus, E. coli, B. subtilis, Aeromonas hydrophila, Vibrio fischeri, and Vibrio harveyi [164]. Cladophora glomerata exhibited significant antibacterial activity against the MDR bacterium Acinetobacter baumannii and various human and fish pathogens, such as E. coli, B. cereus, Vibrio anguillarum, V. fischeri, Vibrio parahaemolyticus, and Vibrio vulnificus [165]. An acetylenic sesquiterpene isolated from Caulerpa prolifera, caulerpenyne ( Figure 4H), exhibited antifouling activity against bacteria and the microalga Phaeodactylum tricornutum [166]. Moreover, the ethanolic extracts of C. decorticatum showed antibacterial activity [155] Anticoagulant Activity The earliest report on the anticoagulant effects of substances produced by green seaweeds were carried out on Codium, in particular on C. fragile ssp. tomentosoides [167]. Matsubara also reported on the anticoagulant activity of a sulfated galactan in the Codium genus [168]. Furthermore, Maeda described that sulfated polysaccharides from the marine green alga M. nitidum yielded a sixfold higher anticoagulant effect than heparin [169]. Also, Synytsya reported on the anticoagulant activity of sulfated polysaccharides derived from C. fulvescens [170].
The findings presented above suggest that green marine algae can be considered a promising source of bioactive substances, which should be further studied and exploited for pharmaceutical applications. Some of the biological activities of compounds extracted from marine seaweeds are presented in Table 2.

Concluding Remarks
The marine environment represents a unique source of bioactive compounds with high pharmaceutical potential. Preclinical and clinical studies are in progress for a number of marine organism derivatives. Nevertheless, several problems should be solved for a deep characterization of biomolecules derived from marine microorganisms, micro, and macroalgae. Notably, marine microbes are notoriously difficult to prepare and maintain in culture. Bacteria likely grow as consortia in the marine environment and dependences on other bacteria for essential nutrients should be guaranteed. These interactions are lacking in isolated laboratory cultures. Further, the marine shear stress, hypersalinity, and antagonists, which induce unique metabolite elaborations, are missing in laboratory cultures. That notwithstanding, these efforts may lead to valuable results. In fact, marine bacteria are significant reservoirs of a plethora of bioactive molecules which have never been found in terrestrial organisms. Moreover, microalgae represent an important, still underestimated source of bioactive metabolites, such as antiviral and anti-AIDS agents. Seaweeds as well produce many different, interesting biologically active substances, such as sulphated polysaccharides, which are promising compounds for drug development [171]. For example, carbohydrate recognition molecules, such as lectins from green seaweeds, have the potential to be used as antitumor and antiviral agents, but they have been rarely investigated [172]. Notably, a natural compound can have variable effects on the human body, as host factors are different. The promising results obtained by an in vitro experiment may not show the same effects in a patient's body. Moreover, the human body under an inflammatory process presents an activated immune system that can consider even a therapeutic compound a threat. Thus, proper safety assessments of the studied metabolites are required. The optimal dosage determination is very important, as this parameter distinguishes whether a compound will act as a drug or a poison. From a technological point of view, novel cost-effective and large-scale fermentation strategies ought to be devised, and particular attention should be paid to the conditions under which the secondary metabolites are produced. Recreating the physical, chemical, and biological conditions of a marine environment is hardly an achievable task. Metabolic and protein engineering can improve the efficacy of some of the marine candidates with pharmaceutical potential. In the future, the efforts and advances in this direction will certainly open the way for extraordinary discoveries for novel applications of marine-derived compounds in biotechnology and biomedicine.