AhlX, an N-acylhomoserine Lactonase with Unique Properties
AbstractN-Acylhomoserine lactonase degrades the lactone ring of N-acylhomoserine lactones (AHLs) and has been widely suggested as a promising candidate for use in bacterial disease control. While a number of AHL lactonases have been characterized, none of them has been developed as a commercially available enzymatic product for in vitro AHL quenching due to their low stability. In this study, a highly stable AHL lactonase (AhlX) was identified and isolated from the marine bacterium Salinicola salaria MCCC1A01339. AhlX is encoded by a 768-bp gene and has a predicted molecular mass of 29 kDa. The enzyme retained approximately 97% activity after incubating at 25 °C for 12 days and ~100% activity after incubating at 60 °C for 2 h. Furthermore, AhlX exhibited a high salt tolerance, retaining approximately 60% of its activity observed in the presence of 25% NaCl. In addition, an AhlX powder made by an industrial spray-drying process attenuated Erwinia carotovora infection. These results suggest that AhlX has great potential for use as an in vitro preventive and therapeutic agent for bacterial diseases. View Full-Text
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Liu, P.; Chen, Y.; Shao, Z.; Chen, J.; Wu, J.; Guo, Q.; Shi, J.; Wang, H.; Chu, X. AhlX, an N-acylhomoserine Lactonase with Unique Properties. Mar. Drugs 2019, 17, 387.
Liu P, Chen Y, Shao Z, Chen J, Wu J, Guo Q, Shi J, Wang H, Chu X. AhlX, an N-acylhomoserine Lactonase with Unique Properties. Marine Drugs. 2019; 17(7):387.Chicago/Turabian Style
Liu, Pengfu; Chen, Yan; Shao, Zongze; Chen, Jianwei; Wu, Jiequn; Guo, Qian; Shi, Jiping; Wang, Hong; Chu, Xiaohe. 2019. "AhlX, an N-acylhomoserine Lactonase with Unique Properties." Mar. Drugs 17, no. 7: 387.
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