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Open AccessArticle

AhlX, an N-acylhomoserine Lactonase with Unique Properties

1
Collaborative Innovation Center of Yangtze River DeltaRegion Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China
2
Shanghai Advanced Research Institute, Chinese Academy of Sciences, Pudong, Shanghai 201210, China
3
Key Laboratory of Marine Biogenetic Resources, The Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China
*
Authors to whom correspondence should be addressed.
Mar. Drugs 2019, 17(7), 387; https://doi.org/10.3390/md17070387
Received: 23 May 2019 / Revised: 12 June 2019 / Accepted: 25 June 2019 / Published: 28 June 2019
N-Acylhomoserine lactonase degrades the lactone ring of N-acylhomoserine lactones (AHLs) and has been widely suggested as a promising candidate for use in bacterial disease control. While a number of AHL lactonases have been characterized, none of them has been developed as a commercially available enzymatic product for in vitro AHL quenching due to their low stability. In this study, a highly stable AHL lactonase (AhlX) was identified and isolated from the marine bacterium Salinicola salaria MCCC1A01339. AhlX is encoded by a 768-bp gene and has a predicted molecular mass of 29 kDa. The enzyme retained approximately 97% activity after incubating at 25 °C for 12 days and ~100% activity after incubating at 60 °C for 2 h. Furthermore, AhlX exhibited a high salt tolerance, retaining approximately 60% of its activity observed in the presence of 25% NaCl. In addition, an AhlX powder made by an industrial spray-drying process attenuated Erwinia carotovora infection. These results suggest that AhlX has great potential for use as an in vitro preventive and therapeutic agent for bacterial diseases. View Full-Text
Keywords: quorum sensing; quorum-quenching; N-acylhomoserine lactonase; marine; thermostable; salt tolerance quorum sensing; quorum-quenching; N-acylhomoserine lactonase; marine; thermostable; salt tolerance
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Liu, P.; Chen, Y.; Shao, Z.; Chen, J.; Wu, J.; Guo, Q.; Shi, J.; Wang, H.; Chu, X. AhlX, an N-acylhomoserine Lactonase with Unique Properties. Mar. Drugs 2019, 17, 387.

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