A Glycosaminoglycan Extract from Portunus pelagicus Inhibits BACE1, the β Secretase Implicated in Alzheimer’s Disease

Therapeutic options for Alzheimer’s disease, the most common form of dementia, are currently restricted to palliative treatments. The glycosaminoglycan heparin, widely used as a clinical anticoagulant, has previously been shown to inhibit the Alzheimer’s disease-relevant β-secretase 1 (BACE1). Despite this, the deployment of pharmaceutical heparin for the treatment of Alzheimer’s disease is largely precluded by its potent anticoagulant activity. Furthermore, ongoing concerns regarding the use of mammalian-sourced heparins, primarily due to prion diseases and religious beliefs hinder the deployment of alternative heparin-based therapeutics. A marine-derived, heparan sulphate-containing glycosaminoglycan extract, isolated from the crab Portunus pelagicus, was identified to inhibit human BACE1 with comparable bioactivity to that of mammalian heparin (IC50 = 1.85 μg mL−1 (R2 = 0.94) and 2.43 μg mL−1 (R2 = 0.93), respectively), while possessing highly attenuated anticoagulant activities. The results from several structural techniques suggest that the interactions between BACE1 and the extract from P. pelagicus are complex and distinct from those of heparin.


Introduction
Alzheimer's disease (AD), the most common form of dementia, is characterized by progressive neurodegeneration and cognitive decline [1]. The deposition and aggregation of toxic amyloid-β proteins (Aβ), the primary constituents of β-amyloid plaques, has been identified as one of the primary causative factors in the development of AD. Approximately 270 mutations within genes that are directly associated with Aβ production are currently linked to the early-onset development of AD [2]. Furthermore, additional genetic risk factors for late-onset AD have been identified, most notably the APOE polymorphism [1]. Other pathological hallmarks of AD include the presence of intraneuronal neurofibrillary tangles (NFTs), an enhanced inflammatory response, neurotransmitter depletion, metal In order to corroborate the Hp/HS like structural characteristics of P. pelagicus F5, the ATR-FTIR spectra has been compared with that of Hp. Both P. pelagicus F5 and Hp were shown to share similar spectral features, for instance bands at 1230, 1430 and 1635 cm −1 , which are associated with S=O stretches, symmetric carbonyl stretching and asymmetric stretches, respectively, indicative of common structural motifs. An additional peak and a peak shoulder located at ~1750 and ~1370 cm −1 were observed in P. pelagicus F5, but absent in Hp. The peak shoulder at ~1370 cm −1 is indicative of a Hp and CS mixture. The differences observed between the spectra of P. pelagicus F5 and Hp in the variable OH region (>3000 cm −1 ) are likely to be associated with changeable moisture levels present during sample acquisition (Figure 2A) as opposed to underlying differences within the glycan structure [41].
Post-acquisition, the ATR-FTIR spectrum of P. pelagicus F5 was interrogated against a library of known GAGs comprising: 185 Hps, 31 HSs, 44 CSs and DSs, 11 hyaluronic acids (HAs) and 6 In order to corroborate the Hp/HS like structural characteristics of P. pelagicus F5, the ATR-FTIR spectra has been compared with that of Hp. Both P. pelagicus F5 and Hp were shown to share similar spectral features, for instance bands at 1230, 1430 and 1635 cm −1 , which are associated with S=O stretches, symmetric carbonyl stretching and asymmetric stretches, respectively, indicative of common structural motifs. An additional peak and a peak shoulder located at~1750 and~1370 cm −1 were observed in P. pelagicus F5, but absent in Hp. The peak shoulder at~1370 cm −1 is indicative of a Hp and CS mixture. The differences observed between the spectra of P. pelagicus F5 and Hp in the variable OH region (>3000 cm −1 ) are likely to be associated with changeable moisture levels present during sample acquisition (Figure 2A) as opposed to underlying differences within the glycan structure [41].
Post-acquisition, the ATR-FTIR spectrum of P. pelagicus F5 was interrogated against a library of known GAGs comprising: 185 Hps, 31 HSs, 44 CSs and DSs, 11 hyaluronic acids (HAs) and 6 oversulphated chondroitin sulphates (OSCSs) using principal component analysis (PCA; Figure 2B) [41]. Principal component 1 (PC1), which covers 57% of the total variance, indicates that P. pelagicus F5 locates within the region containing mammalian Hp/HS. Through comparison of PC1 and PC2, comprising >70% of the total variance, P. pelagicus F5 lies towards the CS region, a location previously identified with Hps containing small amounts of CS/DS [41] analogous to crude, pharmaceutical Hp.  Figure 2B) [41]. Principal component 1 (PC1), which covers 57% of the total variance, indicates that P. pelagicus F5 locates within the region containing mammalian Hp/HS. Through comparison of PC1 and PC2, comprising >70% of the total variance, P. pelagicus F5 lies towards the CS region, a location previously identified with Hps containing small amounts of CS/DS [41] analogous to crude, pharmaceutical Hp. P. pelagicus F5 was subsequently subjected to exhaustive enzymatic cleavage with Flavobacterium heparinum lyases I, II and III. The digest products from Hp control ( Figure 3, Table 1) and P. pelagicus F5 ( Figure 4, Table 1), were analysed using strong anion-exchange chromatography and the retention times compared to those of the eight common ∆-disaccharide standards present within both Hp and HS [42].
The digest products detected for Hp were in agreement with a typical mammalian Hp disaccharide profile [42], with 51.5% of the total products attributable to the trisulphated ∆-UA(2S)-GlcNS(6S) and 22.9% to ∆-UA-GlcNS(6S). A minimal proportion of mono-or unsulphated disaccharides, accounting for 12.3 and 4.3%, respectively, were also observed for Hp. In comparison, a more disperse sulphation profile was observed for P. pelagicus F5 than Hp (Table 1), with a comparatively lower proportion of trisulphated disaccharides, 23.1%. The P. pelagicus F5 contained 24.4% monosulphated disaccharides, of which 16.5% was accounted for by ∆-UA(2S)-GlcNAc. A higher proportion of ∆-UA(2S)-GlcNS (23.5%) was also detected in P. pelagicus F5 than Hp (5.9%), indicating that the compound displays distinct structural characteristics. Such features also contrast with that of HS, where ~50-70% of disaccharides are comprised of ∆-UA-GlcNAc/∆-UA-GlcNS [42][43][44][45]. Also, P. pelagicus F5 presents a significant higher proportion of trisulphated disaccharides than commonly present in mammalian HS, a typical marker of more heparin-like structures. P. pelagicus F5 was subsequently subjected to exhaustive enzymatic cleavage with Flavobacterium heparinum lyases I, II and III. The digest products from Hp control ( Figure 3, Table 1) and P. pelagicus F5 ( Figure 4, Table 1), were analysed using strong anion-exchange chromatography and the retention times compared to those of the eight common ∆-disaccharide standards present within both Hp and HS [42].
The digest products detected for Hp were in agreement with a typical mammalian Hp disaccharide profile [42], with 51.5% of the total products attributable to the trisulphated ∆-UA(2S)-GlcNS(6S) and 22.9% to ∆-UA-GlcNS(6S). A minimal proportion of mono-or unsulphated disaccharides, accounting for 12.3 and 4.3%, respectively, were also observed for Hp. In comparison, a more disperse sulphation profile was observed for P. pelagicus F5 than Hp (Table 1), with a comparatively lower proportion of trisulphated disaccharides, 23.1%. The P. pelagicus F5 contained 24.4% monosulphated disaccharides, of which 16.5% was accounted for by ∆-UA(2S)-GlcNAc. A higher proportion of ∆-UA(2S)-GlcNS (23.5%) was also detected in P. pelagicus F5 than Hp (5.9%), indicating that the compound displays distinct structural characteristics. Such features also contrast with that of HS, where~50-70% of disaccharides are comprised of ∆-UA-GlcNAc/∆-UA-GlcNS [42][43][44][45]. Also, P. pelagicus F5 presents a significant higher proportion of trisulphated disaccharides than commonly present in mammalian HS, a typical marker of more heparin-like structures.             Proton and Heteronuclear Single-Quantum Correlation (HSQC) NMR was employed to confirm the GAGs composition of P. pelagicus F5. 1 H NMR can indicate the major signals associated with HS as well as signals that arise from galactosaminoglycans such as CS. The presence of both ( Figure 5A insert) is easily identified by the two N-acetyl signals at 2.02 ppm (CS) and 2.04 ppm (HS). 1 H-13 C HSQC NMR ( Figure 5B) has been used to resolve overlapping signals and saccharide composition estimates using peak volume integration. The integration of N-acetyl signals revealed that the extract is composed of approximately 60% HS and 40% CS. The combined integration of the N-acetyl and A2 signals from the HS showed that P. pelagicus F5 possesses a high NS content of approximately 76%, which supports the HPLC-based empirical disaccharide analysis ( Figure 4 and Table 1). Together, this data establishes that the HS of P. pelagicus F5 is considerably more sulphated (Table 1) than that commonly extracted from mammalian sources [45]. With regard to the CS element of P. pelagicus F5, signals typical of the CS backbone are present although sulhation is generally low, with galactosamine 6-O-sulphation occurring in approximately 35% of all CS residues. The lack of non-overlapping signals for galactosamine 4-O-sulphation indicates that all but negligible levels of such a modification are present within the CS component.  Proton and Heteronuclear Single-Quantum Correlation (HSQC) NMR was employed to confirm the GAGs composition of P. pelagicus F5. 1 H NMR can indicate the major signals associated with HS as well as signals that arise from galactosaminoglycans such as CS. The presence of both ( Figure 5A insert) is easily identified by the two N-acetyl signals at 2.02 ppm (CS) and 2.04 ppm (HS). 1 H-13 C HSQC NMR ( Figure 5B) has been used to resolve overlapping signals and saccharide composition estimates using peak volume integration. The integration of N-acetyl signals revealed that the extract is composed of approximately 60% HS and 40% CS. The combined integration of the N-acetyl and A2 signals from the HS showed that P. pelagicus F5 possesses a high NS content of approximately 76%, which supports the HPLC-based empirical disaccharide analysis ( Figure 4 and Table 1). Together, this data establishes that the HS of P. pelagicus F5 is considerably more sulphated (Table 1) than that commonly extracted from mammalian sources [45]. With regard to the CS element of P. pelagicus F5, 90.7 ± 2.9% (n = 3) was observed in the presence of 5 µg mL −1 P. pelagicus F5, with an IC 50 value of 1.9 µg mL −1 (R 2 = 0.94). This was comparable to the activity of Hp, which exhibited a maximal level of BACE1 inhibition of 92.5 ± 1.5% (n = 3) at 5 µg mL −1 , with an IC 50 of 2.4 µg mL −1 (R 2 = 0.93).
In the presence of low concentrations of Hp, an increase in BACE1 activity was observed ( Figure 6A,B), with maximal activation occurring at 625 ng mL −1 (57.5 ± 3.7%, n = 3). The BACE1 utilised in this study consisted of the zymogen form (Thr 22 -Thr 457 ), containing the prodomain sequence. This is in accordance with previous reports that demonstrate low concentrations (~1 µg mL −1 ) of heparin can stimulate proBACE1 activity [46,47]. A maximum increase in BACE1 activity was also detected in the presence of 625 ng mL -1 of P. pelagicus F5 (38.5 ± 1.4%, n = 3), although significantly diminished promotion was displayed compared to the same concentration of Hp (57.5 ± 3.7%, n = 3); t(4) = 4.859, p = 0.0083. This indicates that although P. pelagicus F5 exhibits stimulatory activity, it is significantly less than that of Hp. The percent activity level returned to that of the negative control value at concentrations lower than 312.5 ng mL −1 , indicating that both inhibitory and stimulatory effects are dose dependent. For both Hp and P. pelagicus F5, BACE1 promotion was followed by enzyme inhibition, as previously reported ( Figure 6B,C; [47]). The rate of BACE1 activity between 60 and 90 min was significantly different from controls lacking either Hp (n = 3-6; t(4) = 7, p < 0.003) or P. pelagicus F5 (n = 3-6; t(6) = 7, p < 0.004) at 625 ng mL −1 , indicating that inhibition was not due to substrate limitations.
In the presence of low concentrations of Hp, an increase in BACE1 activity was observed ( Figure  6A,B), with maximal activation occurring at 625 ng mL −1 (57.5 ± 3.7%, n = 3). The BACE1 utilised in this study consisted of the zymogen form (Thr 22 -Thr 457 ), containing the prodomain sequence. This is in accordance with previous reports that demonstrate low concentrations (~1 μg mL −1 ) of heparin can stimulate proBACE1 activity [46,47]. A maximum increase in BACE1 activity was also detected in the presence of 625 ng mL -1 of P. pelagicus F5 (38.5 ± 1.4%, n = 3), although significantly diminished promotion was displayed compared to the same concentration of Hp (57.5 ± 3.7%, n = 3); t(4) = 4.859, p = 0.0083. This indicates that although P. pelagicus F5 exhibits stimulatory activity, it is significantly less than that of Hp. The percent activity level returned to that of the negative control value at concentrations lower than 312.5 ng mL −1 , indicating that both inhibitory and stimulatory effects are dose dependent. For both Hp and P. pelagicus F5, BACE1 promotion was followed by enzyme inhibition, as previously reported ( Figure 6B,C; (47). The rate of BACE1 activity between 60 and 90 min was significantly different from controls lacking either Hp (n = 3-6; t(4) = 7, p < 0.003) or P. pelagicus F5 (n = 3-6; t(6) = 7, p < 0.004) at 625 ng mL −1 , indicating that inhibition was not due to substrate limitations.

Heparin Binding Induces a Conformational Change in the Alzheimer's Disease β-Secretase, BACE1
Hp binding has been proposed to occur at a location close to the active site of BACE1 [18], possibly within or adjacent to the prodomain sequence [46]. In light of the contrasting and concentration-dependant BACE1:GAG bioactivities, the ability of Hp and P. pelagicus F5 to induce structural changes in BACE1 has been investigated utilising circular dichroism (CD) spectroscopy at a range of w/w ratios; this also negates the intrinsic effect of the significant polydispersity for this class of biomolecules.
The CD spectra of BACE1 at pH 4.0 has previously been shown to contain a greater proportion of β-sheet and reduced α-helical content, compared to spectra obtained at pH 7.4, indicating that at an acidic pH, where BACE1 is most active, a conformational change can be observed by CD [50]. Consistent with this, the CD spectra of BACE1 in 50 mM sodium acetate buffer at pH 4.0 (Figures 7 and 8) featured a positive peak at wavelengths below 200 nm, which can be attributed to a sum of α-helical and βsheet structures [51]. The broad, negative band observed between wavelengths 250 and 200 nm, contains a peak at λ = 218 nm~208 nm, commonly associated with antiparallel β-sheets and α-helical structures, respectively [51] (Figures 7 and 8). The CD spectra of BACE1 at pH 4.0 can be estimated to have a secondary structural composition of 9% α-helix, 31% antiparallel β-sheet, 16% turn and 44% other (NRMSD < 0.1) when fitted against a library of representative proteins using BeStSel [48]. This was in close agreement with the BestSel secondary structure prediction based on x-ray crystallography of BACE1 at pH 4.0 (PDB accession no 2ZHS, [52] of 7% α-helix, 30% antiparallel, 4% parallel, 12% turn and 47% other). Deviations between secondary structure predictions may be accounted for by subtle differences present within the BACE1 primary sequences.

Heparin Binding Induces a Conformational Change in the Alzheimer's Disease β-Secretase, BACE1
Hp binding has been proposed to occur at a location close to the active site of BACE1 [18], possibly within or adjacent to the prodomain sequence [46]. In light of the contrasting and concentrationdependant BACE1:GAG bioactivities, the ability of Hp and P. pelagicus F5 to induce structural changes in BACE1 has been investigated utilising circular dichroism (CD) spectroscopy at a range of w/w ratios; this also negates the intrinsic effect of the significant polydispersity for this class of biomolecules.  [48]. % structural change of B:Hp; 1:2 or 2:1 (w/w) ratio are highlighted in grey. (C) CD spectra of BACE1 alone (solid line) with Hp (dashed line) at a ratio of 2:1 w/w (D) Near-UV CD spectra of (C); respective absorption regions of aromatic amino acids are indicated [49]. Spectra were recorded in 50 mM sodium acetate buffer at pH 4.0 in all panels.
The CD spectra of BACE1 at pH 4.0 has previously been shown to contain a greater proportion of β-sheet and reduced α-helical content, compared to spectra obtained at pH 7.4, indicating that at an acidic pH, where BACE1 is most active, a conformational change can be observed by CD [50]. Consistent with this, the CD spectra of BACE1 in 50 mM sodium acetate buffer at pH 4.0 (Figure 7 and 8) featured a positive peak at wavelengths below 200 nm, which can be attributed to a sum of α-helical and β-sheet structures [51]. The broad, negative band observed between wavelengths 250 and 200 nm, contains a peak at λ = 218 nm ~ 208 nm, commonly associated with antiparallel β-sheets and α-helical structures, respectively [51] (Figures 7 and 8). The CD spectra of BACE1 at pH 4.0 can be estimated to have a secondary structural composition of 9% α-helix, 31% antiparallel β-sheet, 16% turn and 44% other (NRMSD < 0.1) when fitted against a library of representative proteins using BeStSel [48]. This α-helix (black), antiparallel (red), parallel (blue), turn (magenta) and others (green) [48]. % structural change of B:Hp; 1:2 or 2:1 (w/w) ratio are highlighted in grey. (C) CD spectra of BACE1 alone (solid line) with Hp (dashed line) at a ratio of 2:1 w/w (D) Near-UV CD spectra of (C); respective absorption regions of aromatic amino acids are indicated [49]. Spectra were recorded in 50 mM sodium acetate buffer at pH 4.0 in all panels.
In the presence of a BACE1:Hp (B:Hp), ratio of 1:2 (w/w) where maximal inhibition was observed in FRET assays, the CD spectra of BACE1 exhibited increased negative ellipticity below λ = 222 nm, Mar. Drugs 2019, 17, 293 9 of 20 resulting in an estimated increase in α-helix (+6%) and a reduction in antiparallel β-sheet (−8%) (NRMSD < 0.1) [48] (Figure 7A,B). In comparison to Hp, BACE1 in the presence of P. pelagicus F5 (B:F5), at the same ratio (1:2; w/w), exhibited a slight increase in positive ellipticity between λ = 222-200 nm and decreases at λ < 200 nm, resulting in an estimated change in α-helical content of +1% accompanied by a decrease in antiparallel β-sheet content of 8% ( Figure 8A,B). This is in contrast to CD studies in the presence of peptide inhibitors, which did not reveal a secondary structural change in BACE1 [50].
The conformational change of BACE1 upon binding to Hp and P. pelagicus F5 was assessed over a range of ratios ( Figures 7B and 8B). At a B:Hp ratio of 2:1 (w/w), a change in the characteristics of the CD spectrum of BACE1 was observed in the far-UV region (λ < 250 nm; Figure 7C) that was identified as a reduction in α-helix by 6% and an increase in antiparallel β-sheet structures 19% (NRMSD < 0.1) [48]. In addition, an increase in positive ellipticity was observed in the near-UV region (250-300 nm; Figure 7C,D) following the addition of Hp, which may be attributed to a change in the tertiary structure of BACE1 involving aromatic amino acids [49,53]. In contrast, B:F5 at the same ratio of 2:1 (w/w), exhibited a decrease in ellipticity in the near-and far-UV region (λ < 300 nm; Figure S1).
The increase in positive ellipticity observed in the CD spectra of BACE1 in the near-UV region at a B:Hp ratio of 2:1 (w/w) was also observed at a 1:1 (w/w) ratio of B:F5 ( Figure 8C,D). The secondary structural change in the far-UV CD spectrum of BACE1 at a B:F5 ratio of 1:1 (w/w) for λ= 250-190 nm corresponded to a decrease in α-helix by 8% and an increase in antiparallel β-sheet structures by 15%. and 47% other). Deviations between secondary structure predictions may be accounted for by subtle differences present within the BACE1 primary sequences.
In the presence of a BACE1:Hp (B:Hp), ratio of 1:2 (w/w) where maximal inhibition was observed in FRET assays, the CD spectra of BACE1 exhibited increased negative ellipticity below λ = 222 nm, resulting in an estimated increase in α-helix (+6%) and a reduction in antiparallel β-sheet (−8%) (NRMSD < 0.1) [48] (Figure 7A,B). In comparison to Hp, BACE1 in the presence of P. pelagicus F5 (B:F5), at the same ratio (1:2; w/w), exhibited a slight increase in positive ellipticity between λ = 222-200 nm and decreases at λ < 200 nm, resulting in an estimated change in α-helical content of +1% accompanied by a decrease in antiparallel β-sheet content of 8% ( Figure 8A,B). This is in contrast to CD studies in the presence of peptide inhibitors, which did not reveal a secondary structural change in BACE1 [50].
The conformational change of BACE1 upon binding to Hp and P. pelagicus F5 was assessed over a range of ratios ( Figures 7B and 8B). At a B:Hp ratio of 2:1 (w/w), a change in the characteristics of the CD spectrum of BACE1 was observed in the far-UV region (λ < 250 nm; Figure 7C) that was identified as a reduction in α-helix by 6% and an increase in antiparallel β-sheet structures 19% (NRMSD < 0.1) [48]. In addition, an increase in positive ellipticity was observed in the near-UV region (250-300 nm; Figure 7C,D) following the addition of Hp, which may be attributed to a change in the tertiary structure of BACE1 involving aromatic amino acids [49,53]. In contrast, B:F5 at the same ratio of 2:1 (w/w), exhibited a decrease in ellipticity in the near-and far-UV region (λ < 300 nm; supplementary data).
The increase in positive ellipticity observed in the CD spectra of BACE1 in the near-UV region at a B:Hp ratio of 2:1 (w/w) was also observed at a 1:1 (w/w) ratio of B:F5 ( Figure 8C,D). The secondary structural change in the far-UV CD spectrum of BACE1 at a B:F5 ratio of 1:1 (w/w) for λ= 250-190 nm corresponded to a decrease in α-helix by 8% and an increase in antiparallel β-sheet structures by 15%.   [49]. Spectra were recorded in 50 mM sodium acetate buffer at pH 4.0 in all panels.

Heparin and P. pelagicus F5 Destabilise the Alzheimer's Disease β-Secretase, BACE1
Both Hp and P. pelagicus F5 were shown to induce a conformational change in BACE1, in contrast to previous CD studies in the presence of peptide inhibitors [50]. Therefore, to explore whether the binding of Hp or P. pelagicus F5 alters the stability of BACE1 in a mechanism similar to known inhibitors, differential scanning fluorimetry (DSF) was employed to monitor the change in thermal stability. Previously identified BACE1 inhibitors have been shown to stabilize BACE1, exemplified by an increase in T M values obtained through DSF measurements [54]. In the presence of a BACE1:Hp or P. pelagicus F5 ratio of 1:2, a decrease in the T M of BACE1 by 11 • C and 10 • C, respectively was observed ( Figure 9A). The change in T M of BACE1 induced by binding of either Hp or P. pelagicus F5 was not significantly different, (p = 0.1161 t= 2 df = 4). The destabilisation of BACE1 in the presence of both Hp and P. pelagicus F5 was found to be concentration dependent ( Figure 9B).
Mar. Drugs 2019, 17, x FOR PEER REVIEW 10 of 20 line) or with P. pelagicus F5 (dashed line; ratio of 1:1 w/w); (D) Near-UV CD spectra of (C); respective absorption regions of aromatic amino acids are indicated [49]. Spectra were recorded in 50 mM sodium acetate buffer at pH 4.0 in all panels.

Heparin and P. pelagicus F5 Destabilise the Alzheimer's Disease β-Secretase, BACE1
Both Hp and P. pelagicus F5 were shown to induce a conformational change in BACE1, in contrast to previous CD studies in the presence of peptide inhibitors [50]. Therefore, to explore whether the binding of Hp or P. pelagicus F5 alters the stability of BACE1 in a mechanism similar to known inhibitors, differential scanning fluorimetry (DSF) was employed to monitor the change in thermal stability. Previously identified BACE1 inhibitors have been shown to stabilize BACE1, exemplified by an increase in TM values obtained through DSF measurements [54]. In the presence of a BACE1:Hp or P. pelagicus F5 ratio of 1:2, a decrease in the TM of BACE1 by 11 °C and 10 °C, respectively was observed (

Attenuated Anticoagulant Activities of the P. pelagicus Glycosaminoglycan Extract
An important consideration when determining the therapeutic potential of a heparin-like polysaccharide against AD is the likely side effect of anticoagulation. The prothrombin time (PT) and activated partial thromboplastin time (aPTT) of P. pelagicus F5 were measured compared to Hp (193 IU mg −1 ), to determine the overall effect on the extrinsic and intrinsic coagulation pathways, respectively (both assays also include the common coagulation pathway). In comparison to Hp, P. pelagicus F5 exhibited reduced anticoagulant activity in both the PT ( Figure 10A; EC50 of 420.2 μg mL −1 compared to 19.53 μg mL −1 , respectively) and aPTT ( Figure 10B; EC50 43.21 μg mL −1 compared to 1.66 μg mL −1 , respectively) coagulation assays. Both results show that the extract presents a negligible anticoagulant activity.

Attenuated Anticoagulant Activities of the P. pelagicus Glycosaminoglycan Extract
An important consideration when determining the therapeutic potential of a heparin-like polysaccharide against AD is the likely side effect of anticoagulation. The prothrombin time (PT) and activated partial thromboplastin time (aPTT) of P. pelagicus F5 were measured compared to Hp (193 IU mg −1 ), to determine the overall effect on the extrinsic and intrinsic coagulation pathways, respectively (both assays also include the common coagulation pathway). In comparison to Hp, P. pelagicus F5 exhibited reduced anticoagulant activity in both the PT ( Figure 10A; EC 50 of 420.2 µg mL −1 compared to 19.53 µg mL −1 , respectively) and aPTT ( Figure 10B; EC 50

Discussion
The glycosaminoglycan extract isolated from P. pelagicus was observed to possess similar electrophoretic behaviour to mammalian HS and Hp, with no bands identified corresponding to CS or DS standards. In contrast, the FTIR and HSQC analyses of P. pelagicus F5 identified regions corresponding to both HS and CS saccharides within the extract. PCA analysis of the FTIR spectra revealed P. pelagicus F5 contained features associated with both HS/Hp and CS/DS, which are typical of crude heparin preparations [41]. This was confirmed by HSQC NMR, which identified N-acetyl peaks associated with both galactosamine (CS) and glucosamine (HS). The absence of an IdoA signal from the NMR spectra suggests that P. pelagicus F5 resembles HS and CS more closely than DS/Hep [55,56]. Peaks corresponding to Gal-6S and 6-OH were identified by NMR analysis, with no detectable 4-O-sulphation, indicating that the CS component of P. pelagicus F5 resembles CSC saccharides. The HS component possesses >70% N-sulphated moieties, which is greater than mammalian HSs published previously, but is not as heavily N-sulphated as mammalian heparins. An intermediate proportion of trisulphated Δ-disaccharides were also identified post-bacterial lyase digestion in P. pelagicus F5 when compared to mammalian HS and Hp samples. Furthermore, the P. pelagicus F5 extract contained a low proportion of Δ-UA-GlcNAc/Δ-UA-GlcNS, which is typical of more heparinlike structures. This suggests that the HS/Hp component of P. pelagicus F5 consists of a hybrid structure lacking the domain structure of HS and the highly-sulphated regions of Hp.
The absence of a band migrating in a similar manner to that of CS when P. pelagicus F5 was subjected to agarose gel electrophoresis suggests that the polysaccharide is not a mixture of HS and CS chains. The simplicity of the signals in the HSQC spectrum suggests either two separate populations or two distinct domains, while the former is not consistent with the agarose gel electropherogram mentioned previously. The PCA of the FTIR spectra is also in agreement with the presence of discrete, rather than mixed, HS/CS sequences. The precise nature of the arrangement of these stretches remains unknown, although it is well documented that marine-derived GAGs harbour significant and unusual structural features, when compared to those present within their mammalian counterparts [33,43,[57][58][59][60][61][62][63][64][65][66]. Studies to resolve this technically demanding question are currently in progress.
The P. pelagicus F5 extract was found to possess significant inhibitory potential against human BACE1, in a manner akin to that of mammalian Hp, as demonstrated by comparable IC50 concentrations determined via FRET. The ability of P. pelagicus F5 to promote BACE1 bioactivity at lower concentrations, owing to the presence of the BACE1 pro-domain [46,47], appears to be at a diminished level compared to mammalian Hp, suggesting differences between these GAGs and the nature of their interactions with human BACE1. This was exemplified when the secondary structural changes in BACE1 (evident from CD) in the presence of Hp or P. pelagicus F5 were examined.

Discussion
The glycosaminoglycan extract isolated from P. pelagicus was observed to possess similar electrophoretic behaviour to mammalian HS and Hp, with no bands identified corresponding to CS or DS standards. In contrast, the FTIR and HSQC analyses of P. pelagicus F5 identified regions corresponding to both HS and CS saccharides within the extract. PCA analysis of the FTIR spectra revealed P. pelagicus F5 contained features associated with both HS/Hp and CS/DS, which are typical of crude heparin preparations [41]. This was confirmed by HSQC NMR, which identified N-acetyl peaks associated with both galactosamine (CS) and glucosamine (HS). The absence of an IdoA signal from the NMR spectra suggests that P. pelagicus F5 resembles HS and CS more closely than DS/Hep [55,56]. Peaks corresponding to Gal-6S and 6-OH were identified by NMR analysis, with no detectable 4-O-sulphation, indicating that the CS component of P. pelagicus F5 resembles CSC saccharides. The HS component possesses >70% N-sulphated moieties, which is greater than mammalian HSs published previously, but is not as heavily N-sulphated as mammalian heparins. An intermediate proportion of trisulphated ∆-disaccharides were also identified post-bacterial lyase digestion in P. pelagicus F5 when compared to mammalian HS and Hp samples. Furthermore, the P. pelagicus F5 extract contained a low proportion of ∆-UA-GlcNAc/∆-UA-GlcNS, which is typical of more heparin-like structures. This suggests that the HS/Hp component of P. pelagicus F5 consists of a hybrid structure lacking the domain structure of HS and the highly-sulphated regions of Hp.
The absence of a band migrating in a similar manner to that of CS when P. pelagicus F5 was subjected to agarose gel electrophoresis suggests that the polysaccharide is not a mixture of HS and CS chains. The simplicity of the signals in the HSQC spectrum suggests either two separate populations or two distinct domains, while the former is not consistent with the agarose gel electropherogram mentioned previously. The PCA of the FTIR spectra is also in agreement with the presence of discrete, rather than mixed, HS/CS sequences. The precise nature of the arrangement of these stretches remains unknown, although it is well documented that marine-derived GAGs harbour significant and unusual structural features, when compared to those present within their mammalian counterparts [33,43,[57][58][59][60][61][62][63][64][65][66]. Studies to resolve this technically demanding question are currently in progress.
The P. pelagicus F5 extract was found to possess significant inhibitory potential against human BACE1, in a manner akin to that of mammalian Hp, as demonstrated by comparable IC 50 concentrations determined via FRET. The ability of P. pelagicus F5 to promote BACE1 bioactivity at lower concentrations, owing to the presence of the BACE1 pro-domain [46,47], appears to be at a diminished level compared to mammalian Hp, suggesting differences between these GAGs and the nature of their interactions with human BACE1. This was exemplified when the secondary structural changes in BACE1 (evident from CD) in the presence of Hp or P. pelagicus F5 were examined. BACE1 has previously been observed to adopt a unique secondary structure at pH 4.0, where catalytic activity is increased, resulting in a predicted increase in beta-sheet and a reduction in alpha-helical structures [50]. When the changes in the secondary structure (evident from CD) of BACE1 in the presence of high concentrations of Hp (BACE1:Hp ratio of 1:2) was examined, a shift towards the structural features observed for BACE1 alone at pH 7.4 was observed (increase in alpha-helical and reduction in beta sheet structures). At high concentrations (B:F5 ratio of 1:2), the P. pelagicus F5 extract induced similar, but not identical, changes to the secondary structure of human BACE1, when compared to those of Hp at the same ratio.
In contrast, the CD spectra observed for B:Hp complexes under conditions that facilitate BACE1 promotion (i.e., low Hp concentrations) demonstrated evidence of an interaction that involves the aromatic amino acids (near UV CD). Tyr-71 is located within the BACE1 flap that has previously been identified to change conformation between the flap-open and flap-closed states [67]. Unfortunately, due to the location of the aromatic residues on the surface of the protein, it is not possible to conclude definitely whether interaction(s) of Hp-based inhibitors with human BACE1 occur at, or near to, the active site. This interpretation is consistent with the previous reports that a conformational change in BACE1 may occur upon heparin binding, which would be required to allow access into the active site [46]. In addition, the increase in BACE1 activity by heparin has been shown to be followed by BACE1 inhibition [47], which may suggest this arrangement is required to allow access to the active site. The results also support the work by Scholefield et al. [18] who showed that the mode of Hp inhibition is non-competitive, and can prevent access of the substrate.
At lower GAG concentrations, differences in BACE1 secondary structure were observed between the B:Hp and B:F5 complexes in the CD spectra, although a similar change in the near UV CD spectra of BACE1 was observed with increased amounts of P. pelagicus F5. This may be accounted for by the reduced potency of P. pelagicus F5 with regard to activating BACE1, or indicative of an alternative interaction. The conformational change induced in the near-UV CD spectra of BACE1 is solely the result of the HS/Hp-like component of the P. pelagicus F5 extract. CS has previously been shown to possess diminished BACE1 promotion activity compared to Hp/HS [46].
From a mechanistic standpoint, the decrease in the T m s observed using DSF for both the human BACE1 protein in the presence of either Hp or the P. pelagicus F5 extract, when compared to human BACE1 alone, suggests that the mode of BACE1 inhibition by this class of carbohydrates could both involve structural destabilisation. The Hp-induced thermal instability of human BACE1 occurs in a concentration dependent manner, akin to that of the inhibitory potential of Hp in the FRET-based bioactivity assay. As for the FRET-based, BACE1 inhibition assays, P. pelagicus [F5] also induces comparable destabilisation of BACE1 with similar T m values. A graph of BACE1:GAG T m vs. concentration demonstrates similar profiles for the P. pelagicus GAG extract and that of mammalian Hp. The relationship between Hp and P. pelagicus F5 concentration and biological properties that coexists for both FRET-based, BACE1 inhibition and DSF is not mirrored at defined concentrations of Hp and P. pelagicus F5 with regard to their distinct CD spectra and predicted secondary structure. This would suggest that complex and distinct modes of interactions are present.
One of the major obstacles that precludes the use of mammalian Hp compounds as potential BACE1 inhibitors and pharmaceutical candidates in general is that of the significant anticoagulant potential residual within the biomolecule. This anticoagulant potential is afforded by the propensity of Hp to interact with antithrombin and thereby inhibit the human coagulation pathway, which unperturbed, ultimately results in fibrin clot formation. The anticoagulant potential of P. pelagicus F5 has been shown to be highly attenuated in contrast to mammalian Hp, as measured by both the aPTT and PT clotting assays. These coagulation assays are routinely employed, in clinical settings, to screen for the common pathway in combination with either the intrinsic (aPTT) or extrinsic pathways (PT).

Extraction of Glycosaminoglycans from Portunus pelagicus
A total of 2.4 kg Portunus pelagicus tissue (Yeuh Chyang Canned Food Co., Ltd., Nhut Chanh, Vietnam) was homogenised with excess acetone (VWR, UK) and agitated for 24 h at r.t. Defatted P. pelagicus tissue was recovered via centrifugation, 5670 rcf at r.t. for 10 min, and allowed to air dry. The tissue was then subjected to extensive proteolytic digestion (Alcalase ® ; Novozymes, Bagsvaerd, Denmark) using 16

Attenuated FTIR Spectral Analysis of Marine-Derived Glycosaminoglycans
Samples (10 mg, lyophilised) were recorded using a Bruker Alpha I spectrometer in the region of 4000 to 400 cm −1 for 32 scans at a resolution of 2 cm −1 (approx 70 seconds acquisition time), 5 times. Spectral acquisition was carried out using OPUS software (v8.1, Bruker, Coventry, UK) with correction to remove the residual spectrum of the sampling environment.
Spectral processing and subsequent data analyses were performed using an Asus Vivobook Pro (M580VD-EB76, Taipei, Taiwan) equipped with an intel core i7-7700HQ. Spectra were smoothed, employing a Savitzky-Golay smoothing algorithm (R studio v1.1.463; signal package, sgolayfilter), to a 2nd-degree polynomial with 21 neighbours prior to baseline correction employing a 7th-order polynomial and subsequent normalisation (0-1). CO 2 and H 2 O regions were removed prior to further analysis in order to negate environmental variability (<700 cm −1 , between 2000 and 2500 cm −1 and >3600 cm −1 ). Second derivatives plots were calculated using the Savitzky-Golay algorithm, with 41 neighbours and a 2nd-order polynomial.
The normalised and corrected matrix of intensities was subject to PCA using singular value decomposition in R studio (v1.1.463, Boston, MA, USA) with the mean-centred, base prcomp function deployed.

Constituent ∆-Disaccharide Analysis of Hp/HS-Like, Marine-Derived Carbohydrates
Pharmaceutical (API) grade, porcine intestinal mucosal heparin (193 IU mg −1 ; Celsus, Cincinnati, OH, USA) and P. pelagicus F5 carbohydrate samples were re-suspended in lyase digestion buffer (50 µL; 25 mM sodium acetate, 5 mM calcium acetate (VWR, Lutterworth, UK), pH 7) prior to exhaustive digestion by the sequential addition of a cocktail of the three recombinantly expressed heparinase enzymes (I, III and II) from the soil bacterium Flavobacterium heparinum (2.5 mIU mg −1 ; Iduron, Alderley Edge, UK). Samples were incubated for 4 h at 37 • C prior to a further addition of the same quantity of enzymes and an additional overnight incubation. Samples were then heated briefly at 95 • C post-enzyme digestion (5 min) and allowed to cool.
Denatured heparinase enzymes were removed from the sample solution by immobilisation upon a pre-washed (50% methanol (aq.) followed by HPLC-grade H 2 O) C 18 spin column (Pierce, Altrincham, UK), whereby the newly liberated ∆-disaccharides were present in the column eluate upon washing with HPLC-grade H 2 O.
Lyophilised ∆-disaccharide samples from Hp and P. pelagicus F5 were desalinated by immobilisation up on graphite spin columns (Pierce, Altrincham, UK) that had been extensively prewashed with 80% acetonitrile, 0.5% (aq.) trifluoroacetic acid and HPLC-grade H 2 O prior to use. ∆-disaccharides liberated from the exhaustive, heparinase digestion were separated from buffer salts by extensive washing with HPLC-grade H 2 O prior to elution with a solution of 40% acetonitrile, 0.5% trifluoroacetic acid (aq.). Contaminant, non ∆-disaccharide components of the spin column eluate were removed by serial lyophilization prior to chromatographic separation, using high performance anion exchange chromatography (HPAEC).
Heparinase digested samples (50 µg) were made up in HPLC-grade H 2 O (1 mL) immediately before injection onto a ProPac PA-1 analytical column (4 × 250 mm, ThermoFisher Scientific, Altrincham, UK), and pre-equilibrated in HPLC-grade H 2 O at a flow rate of 1 mL min −1 . The column was held under isocratic flow for 10 min prior to developing a linear gradient from 0 to 2 M NaCl (HPLC grade; VWR, UK) over 60 min. Eluted ∆-disaccharides were detected absorbing within the UV range λ abs = 232 nm via the unsaturated C=C bond, present between C 4 and C 5 of the uronic acid residues, introduced as a consequence of lyase digestion.
Authentic ∆-disaccharide reference standards, comprising the 8 common standards found in Hp and HS (Iduron, Alderley Edge, UK), were employed as a mixture (each at 5 µg mL −1 ) and served as chromatographic references with elution times cross-correlated with Hp and P. pelagicus F5 samples. The column was washed extensively with 2 M NaCl and HPLC-grade H 2 O prior to use and between runs. 4.6. Determination of Human BACE1 Inhibitory Activity Using Förster Resonance Energy Transfer P. pelagicus F5 and Hp were assayed for inhibitory potential against human β-secretase, tag free (BACE1; ACRO Biosystems, Cambridge, MA, USA), using the fluorescence resonance energy transfer (FRET) inhibition assay, essentially as described by Patey et al. (2006) [19]. Human BACE1 (312.5 ng), and P. pelagicus F5 or Hp were incubated in 50 mM sodium acetate at pH 4.0 at 37 • C for 10 min, followed by the addition a quenched fluorogenic peptide substrate (6.25 µM; Biomatik, Cambridge, Ontario, Canada; MCA-SEVNLDAEFRK(DNP)RR-NH 2 ; pre-incubated at 37 • C for 10 min) to a final well volume of 50 µL. Fluorescent emission was recorded using a Tecan Infinite ® M200 Pro multi-well plate reader (Tecan Group Ltd., Männedorf, Switzerland) with i-control™ software (λ ex = 320 nm, λ em = 405 nm) over 90 min. The relative change in fluorescence per minute was calculated in the linear range of the no inhibitor control, with normalized percentage inhibition calculated (% ± SD, n = 3) compared to the x of substrate only and no inhibitor control, followed by fitting to a four-parameter logistics model using Prism 7 (GraphPad Software, San Diego, CA, USA).

Secondary Structure Determination of Human BACE1 by Circular Dichroism Spectroscopy
The circular dichroism (CD) spectra of native, human BACE1 (6.12 µM, 30 µL; Acro Biosystems, USA) in 50 mM sodium acetate (pH 4.0; VWR, Lutterworth, UK) was obtained using a J-1500 Jasco CD spectrometer and Spectral Manager II software, equipped with a 0.2-mm path length quartz cuvette (Hellma, Plainview, NY, USA) operating at a scan speed of 100 nm min −1 with 1-nm resolution over the range λ = 190-320 nm. Spectra obtained were the mean of five independent scans. Human BACE1 was buffer exchanged (in order to remove commercially supplied buffer) prior to spectral analysis using a 10-kDa Vivaspin centrifugal filter (Sartorius, Goettingen, Germany) at 12,000× g washed thrice.
Collected data was processed using Spectral Manager II software and data analysis was carried out with GraphPad Prism 7, employing a 2nd-order polynomial smoothed to 9 neighbours. Secondary structure prediction was performed utilizing the BeStSel analysis server on the unsmoothed data [48]. To ensure the CD spectral change of BACE1 in the presence of each GAG was not altered owing to the addition of the GAG alone, which are known to possess CD spectra at high concentrations [68,69] GAG control spectra were subtracted before analysis. In addition, the theoretical, summative CD spectra was confirmed to differ from the observed experimental CD spectra, thereby indicating that the change in the CD spectra compared to that of BACE1 alone is a result of a conformational change upon binding to the GAG. The conformational change observed is believed to occur as a result of changes solely in BACE1 secondary structure, as GAG controls exhibited negligible spectra at the concentration used. All CD data has been presented with GAG controls subtracted.  [71]. DSF was performed on human BACE1 (1 µg) using 96-well qPCR plates (AB Biosystems, Warrington, UK) with 20X Sypro Orange (Invitrogen, Warrington, UK) in 50 mM sodium acetate, pH 4.0, in a final well volume of 40 µl. Hp or mGAG were included, as necessary, to a maximal concentration of 200 µg mL −1 . An AB Biosystems StepOne plus qPCR machine, with the TAMRA filter set deployed, was used to carry out melt curve experiments, with an initial incubation phase of 2 min at 20 • C increasing by 0.5 • C increments every 30 s up to a final temperature of 90 • C. Data analysis was completed using Prism 7 (GraphPad Software, San Diego, CA, USA) with the first derivative plots smoothed to 19 neighbours, using a 2nd-order polynomial (Savitzky-Golay). The peak of the first derivatives (yielding T m s) was determined using MatLab software (R20018a, MathWorks, Cambridge, UK).

Activated Partial Thromboplastin Time (aPTT)
Serially diluted GAG samples (25 µL) were incubated with pooled, normal human citrated plasma (50 µL; Technoclone, Surrey, UK) and Pathromtin SL reagent (50 µL; Siemens, Erlangen, Germany) for 2 mins at 37 • C prior to the addition of calcium chloride (25 µL, 50 mM; Alfa Aesar, Heysham, UK). The time taken for clot formations to occur (an upper maximal of 2 mins was imposed, represented as 100% inhibition of clotting) was recorded using a Thrombotrak Solo coagulometer (Axis-Shield). HPLC-grade H 2 O (0% inhibition of clotting, representing a normal aPTT clotting time, of ≈ 37-40 seconds) and porcine mucosal heparin (193 IU mg −1 ; Celsus, Cincinnati, OH, USA) were screened as controls. The EC 50 values of all test and control samples were determined using a sigmoidal dose response curve fitted with Prism 7 (GraphPad Software, San Diego, CA, USA).

Prothrombin Time (PT)
Serially diluted GAGs (50 µL) or control (H 2 O, HPLC grade) were incubated with pooled, normal human citrated plasma (50 µL) for 1 minute at 37 • C prior to the addition of Thromborel S reagent (50 µL; Siemens, Erlangen, Germany). The time taken for clot formations to occur (an upper maximal of 2 min was imposed, representing 100% inhibition of clotting) was recorded using a Thrombotrak Solo coagulometer. HPLC-grade H 2 O (0% inhibition of clotting, representing a normal PT clotting time of ≈ 13-14 s) and porcine mucosal heparin (193 IU mg −1 ; Celsus, Cincinnati, OH, USA) were screened as controls. The EC 50 values of all test and control samples were determined using a sigmoidal dose response curve fitted with Prism 7 (GraphPad Software, San Diego, CA, USA).

Conclusions
While the search for effective AD treatments is still on-going, GAGs offer a route to BACE1 inhibition that surmounts the challenge presented by the large substrate-binding cleft of the enzyme and the unfavourable pharmokinetics of peptide-based inhibitors. Heparin, a GAG from mammalian sources (usually porcine or bovine intestinal tissue), has long been known to possess potent BACE1 inhibitory activity [18][19][20] but also exhibits undesirable anticoagulant properties. The P. pelagicus GAG of the present study largely circumvents the anticoagulant limitations of mammalian heparins while maintaining comparable, low IC 50 BACE1 inhibition values. Additional advantages with marine-derived GAGs is that they offer sources, derived from waste material of otherwise very low economic value, that are free from contamination with mammalian pathogens (e.g., BSE) and avoid many of the religious and social issues associated with mammalian products [72]. Interestingly, the mechanism by which the present product from P. pelagicus inhibits BACE1 is complex, concentration dependent and appears to be distinct from that of mammalian heparin, suggesting marine GAGs as a potential starting point for future drug development. Furthermore, as heparin has been shown to positively modulate distinct pathophysiological processes associated with AD [23], this class of molecules may hold the potential for the delivery of a multi-target AD therapeutic. The present contribution highlights the potential offered by the largely unexplored chemical space defined by marine-derived GAGs.