Sorbicillinoid-Based Metabolites from a Sponge-Derived Fungus Trichoderma saturnisporum

Antibacterial activity assessment and high performance liquid chromatography associated with nuclear magnetic resonance (HPLC/NMR) data revealed that the EtOAc extract of the fermented endophytic fungus Trichoderma saturnisporum DI-IA, obtained from the marine sponge Dictyonella incisa, contained conjugated olefinic metabolites with antibacterial activity. Chemical examination of the fungal strain resulted in the isolation of eight new sorbicillinoid-based compounds, namely saturnispols A–H (1–8). Their structures were determined on the basis of extensive spectroscopic analysis, including the experimental and calculated electronic circular dichroism (ECD) data for the configurational assignments. Saturnispol F exerted significant inhibition against a panel of bacteria strains including vancomycin-resistant enterococci (VRE) with a minimum inhibitory concentrations (MIC) ranging from 1.63 to 12.9 μg/mL, while saturnispol H showed selective effects against VRE and B. subtilis.


Introduction
Sorbicillinoids are a class of fungal metabolites with a cyclic hexaketide nucleus, which contains a typical or modified sorbyl sidechain [1,2]. Since the isolation of the first member of the sorbicillinoid family 60 years ago (sorbicillin, from the fungus Penicillium notatum) [3], approximately 100 sorbicillin derivatives have been reported from several strains of fungi obtained from diverse environmental sources. Based on their structural modification and condensation, they are classified into monomers [4], bisorbicillinoids [5][6][7][8], trisorbicillinoids [9,10], and hybrid sorbicillinoids [11][12][13]. These typical natural products possess a range of bioactivities, including cytotoxic [14], antibiotic [15], antiviral [16], antioxidant [17], and lipid-lowering properties [18]. However, few studies have been reported that investigate the structure-activity relationship. Although most sorbicillinoids have originated from terrestrial fungi, marine-derived fungi have proved to be a promising source of derivatives. It is noted that sorbicillinoid-bearing marine fungi are distributed widely, from shallow water to deep sea environments. Among the marine-derived analogues, marine sponge-associated fungi are recognized to produce diverse sorbicillinoid derivatives with unique scaffolds. Sorbifuranones A-C from the sponge-derived fungus Penicillium chrysogenum are a novel class of intriguing sorbicillinol-derived sorbicillinol that lacks a sorbyl sidechain, has antioxidant effects and is produced by the sponge-derived fungus P. citrinum [17], while a group of novel sorbicillinoid polyketide derivatives were isolated from the Caribbean sponge Agelas dispar-associated fungus Trichoderma sp. [20]. Sorbicillinoid alkaloids were first found from a sponge-derived P. chrysogenum strain [16], and vertinoid polyketides with unique scaffolds were isolated from the sponge-associated fungus T. longibrachiatum. These findings demonstrate that sponge-associated fungi are a rich source of structurally unique sorbifuranones.
Vancomycin-resistant enterococci (VRE) have emerged as important hospital-acquired pathogens in immunosuppressed patients and are increasing steadily in occurrence. Antimicrobial therapy is problematic for all VRE, posing serious risks to the health of current and future patients. It is urgent to discover new natural antibiotics to overcome these drug-resistant pathogens. In the course of our search for antibacterial natural products derived from sponge-associated fungi, the marine fungus Trichoderma saturnisporum, which was isolated from the sponge of Dictyonella incise, showed a diverse chemical profile in its HPLC fingerprint and exerted antibacterial effects. This fungal species has rarely been chemically examined, with only one publication reporting peptaibols, namely paracelsins A-E [21]. Several Trichoderma species from terrestrial ecosystems have been registered as biocontrol agents to control fungal plant diseases [22,23]. The fungus T. saturnisporum is an unexploited species for the biocontrol of fungal diseases of plants, and it is a potential antagonist against important plant pathogens [22]. However, the chemical and biological properties of the marine-derived T. saturnisporum are unexplored and rarely described. In the present work, we describe the structural elucidation of eight new sorbicillinoid-based derivatives (Figure 1) from the sponge Dictyonella incisa-associated fungal strain T. saturnisporum and their antibacterial effects.

Results
The HPLC chromatograph in association with the 1 H NMR and electrospray ionization mass spectrometry (ESI-MS) data revealed that the ethyl acetate (EtOAc) extract of the rice fermentation of the fungus T. saturnisporum featured a profile of conjugated olefinic metabolites. The EtOAc extract inhibited Staphylococcus aureus ATCC 29213 with an MIC < 128 μg/mL. Chromatographic separation of the EtOAc extract led to the isolation of eight new sorbicillinoid-related derivatives ( Figure 1).
Saturnispol A (1) was obtained as a red oil. Its molecular formula was determined to be C28H34O10 on the basis of the high resolution electrospray ionization mass spectroscopy (HRESIMS) (m/z 529.2069 [M − H] − , calcd for C28H33O10, 529.2074) and NMR data, requiring 12 degrees of unsaturation. The infrared radiation (IR) absorptions suggested the presence of hydroxy (3380 cm −1 ), carbonyl (1738 cm −1 ), and olefinic (1614 cm −1 ) groups. The 13 C NMR and distortionless enhancement by polarization transfer (DEPT) spectra exhibited a total of 28 carbon resonances, and they were classified into four methyls, three methylenes, nine methines, and 12 quaternary carbons. The connectivity of protons with their associated carbons was assigned by the heteronuclear single quantum coherence (HSQC) spectrum. The proton-proton correlation spectroscopy ( 1 H-1 H COSY)

Results
The HPLC chromatograph in association with the 1 H NMR and electrospray ionization mass spectrometry (ESI-MS) data revealed that the ethyl acetate (EtOAc) extract of the rice fermentation of the fungus T. saturnisporum featured a profile of conjugated olefinic metabolites. The EtOAc extract inhibited Staphylococcus aureus ATCC 29213 with an MIC < 128 µg/mL. Chromatographic separation of the EtOAc extract led to the isolation of eight new sorbicillinoid-related derivatives (Figure 1). Saturnispol A (1) was obtained as a red oil. Its molecular formula was determined to be C 28 H 34 O 10 on the basis of the high resolution electrospray ionization mass spectroscopy (HRESIMS) (m/z 529.2069 [M − H] − , calcd for C 28 H 33 O 10 , 529.2074) and NMR data, requiring 12 degrees of unsaturation. The infrared radiation (IR) absorptions suggested the presence of hydroxy (3380 cm −1 ), carbonyl (1738 cm −1 ), and olefinic (1614 cm −1 ) groups. The 13 C NMR and distortionless enhancement by polarization transfer (DEPT) spectra exhibited a total of 28 carbon resonances, and they were classified into four methyls, three methylenes, nine methines, and 12 quaternary carbons. The connectivity of protons with their associated carbons was assigned by the heteronuclear single quantum coherence (HSQC) spectrum. The proton-proton correlation spectroscopy ( 1 H-1 H COSY) correlations established a side chain for a hydroxylated pentadiene from H-20 (δ H 6.62, d, J = 15.2 Hz) to H 2 -24 (δ H 4.22, d, J = 5.2 Hz). Inspection of the heteronuclear multiple bond correlation (HMBC) data, such as from H 3 -17 (δ H 1.45, s) to C-6 (δ C 168.3), C-5a (δ C 79.2), and C-9a (δ C 53.7), H 3 -18 (δ H 1.43, s) to C-6, C-7 (δ C 109.3), and C-8 (δ C 191.9), as well as the correlations from H-9a (δ H 3.67, s) to C-8, C-9 (δ C 101.7), C-6, and C-5a, resulted in the presence of a cyclohexenone ring, in which Me-17 and Me-18 were positioned at C-5a and C-7, respectively. Additional HMBC correlations from H-20 to C-9 and C-19 (δ C 166.5) deduced the location of a dihydroxylated hexadiene at C-9. Thus, the partial structure of compound 1 closely related to bisvertinol [9] was identified. Further examination of the HMBC and COSY correlations ( Figure 2) established the second moiety for ring C to be a cyclohexanone unit, in which the same side chain as that of the first moiety was located at C-2 (δ C 105.1) based on the HMBC correlations from H 2 -3 (δ H 2.75, 2.47, d, J = 14.6 Hz) and H-11 (δ H 6.42, d, J = 15.2 Hz) to C-2 and C-10 (δ C 177.4). The coexistence of a methyl and a hydroxy group at C-4 (δ C 72.8) was demonstrated by the HMBC correlations from H 3 -16 (δ C 1.22, s) to C-3 (δ C 35.1), C-4, and C-4a (δ C 105.9), while the latter was assigned as a hemiketal carbon. Moreover, the HMBC correlations from H 3 -25 (δ H 1.29) to C-1 (δ C 193.6), C-9b (δ C 59.2), and C-4a established that the methyl group and ketone were positioned at C-9a and C-1, respectively. Thus, the second moiety was determined to be related to that of bisvertinol. Since the assigned moieties required 11 degrees of unsaturation, the remaining one set was suggested to be contributed by a cyclic unit. The HMBC correlations between H 3 -25 and C-9a and from H-9a to C-4a and C-9a, in association with the deshielded shifts of C-4a and C-5a, allowed the formation of a tetrahydrofuran ring between rings A and C. .67, s) to C-8, C-9 (δC 101.7), C-6, and C-5a, resulted in the presence of a cyclohexenone ring, in which Me-17 and Me-18 were positioned at C-5a and C-7, respectively. Additional HMBC correlations from H-20 to C-9 and C-19 (δC 166.5) deduced the location of a dihydroxylated hexadiene at C-9. Thus, the partial structure of compound 1 closely related to bisvertinol [9] was identified. Further examination of the HMBC and COSY correlations ( Figure 2) established the second moiety for ring C to be a cyclohexanone unit, in which the same side chain as that of the first moiety was located at C-2 (δC 105.1) based on the HMBC correlations from H2-3 (δH 2.75, 2.47, d, J = 14.6 Hz) and H-11 (δH 6.42, d, J = 15.2 Hz) to C-2 and C-10 (δC 177.4). The coexistence of a methyl and a hydroxy group at C-4 (δC 72.8) was demonstrated by the HMBC correlations from H3-16 (δC 1.22, s) to C-3 (δC 35.1), C-4, and C-4a (δC 105.9), while the latter was assigned as a hemiketal carbon. Moreover, the HMBC correlations from H3-25 (δH 1.29) to C-1 (δC 193.6), C-9b (δC 59.2), and C-4a established that the methyl group and ketone were positioned at C-9a and C-1, respectively. Thus, the second moiety was determined to be related to that of bisvertinol. Since the assigned moieties required 11 degrees of unsaturation, the remaining one set was suggested to be contributed by a cyclic unit. The HMBC correlations between H3-25 and C-9a and from H-9a to C-4a and C-9a, in association with the deshielded shifts of C-4a and C-5a, allowed the formation of a tetrahydrofuran ring between rings A and C. The relative configuration of compound 1 was inferred from coupling constants and the NOE data. The conjugated double bonds at the side chains were assigned E geometries according to the large J values (15.2 Hz for JH-11/H-12, JH-13/H-14, JH-22/H-23, and JH-20/H-21). The cis fusion of rings A and B was ascribed to the NOE correlation between H3-17 and H-9a, while the cis fusion of rings B and C was suggested by the minimized energy for cis and trans junction of rings B and C, supporting more stability with lower energy for the cis fusion [23]. Additional NOE interactions from H3-25 to H-9a, H-3b (δH 2.47, d, J = 14.6 Hz) and between H-3b and H3-16 revealed H-9a and H3-16 to be cofacial with H3-25 ( Figure 3). Based on the electronic circular dichroism (ECD) exciton chirality rule [24], the negative Cotton effect at 412 nm (∆ε −35.5), and a positive Cotton effect at 332 nm (∆ε +25.5) reflected the chromophores of two enol-sorbyl side chains to adopt a counterclockwise fashion (Figure 4), indicating 9bR configuration. Accordingly, the remaining stereogenic centers were assumed to be 4S, 4aR, 5aS, and 9aR configurations. Comparison of the experimental ECD data with that calculated for the enantiomers of 1 at the B31YP/6-311++G(2d, 2p) level in the gas phase using the B3LYP/6-31G(d) optimized geometries after conformational searches via the MMFF94S force field [25,26] (Figure 4) further supported the configurational assignment. . The cis fusion of rings A and B was ascribed to the NOE correlation between H 3 -17 and H-9a, while the cis fusion of rings B and C was suggested by the minimized energy for cis and trans junction of rings B and C, supporting more stability with lower energy for the cis fusion [23]. Additional NOE interactions from H 3 -25 to H-9a, H-3b (δ H 2.47, d, J = 14.6 Hz) and between H-3b and H 3 -16 revealed H-9a and H 3 -16 to be cofacial with H 3 -25 ( Figure 3). Based on the electronic circular dichroism (ECD) exciton chirality rule [24], the negative Cotton effect at 412 nm (∆ε −35.5), and a positive Cotton effect at 332 nm (∆ε +25.5) reflected the chromophores of two enol-sorbyl side chains to adopt a counterclockwise fashion (Figure 4), indicating 9bR configuration. Accordingly, the remaining stereogenic centers were assumed to be 4S, 4aR, 5aS, and 9aR configurations. Comparison of the experimental ECD data with that calculated for the enantiomers of 1 at the B31YP/6-311++G(2d, 2p) level in the gas phase using the B3LYP/6-31G(d) optimized geometries after conformational searches via the MMFF94S force field [25,26] (Figure 4) further supported the configurational assignment.   (Table 1) and analyses of the two dimensional nuclear magnetic resonance (2D NMR) data revealed that the structure of compound 2 was closely related to that of compound 1. The main difference was due to the presence of a methyl group (δH 1.87, d, J = 6.8 Hz; δC 18.1) at the side chain of ring C to replace the hydroxymethylene group of 1. The COSY relationship between H3-15 (δH 1.87, d, J = 6.8 Hz) and H-14 (δH 6.24, dq, J = 6.8, 15.0 Hz) in association with the HMBC correlations from H3-15 to C-13 (δC 131.1) and C-14 (δC 139.2) supported the methyl location. The similar NOE interactions, such as from H-9a (δH 3.75) to H3-17 (δH 1.45) and H3-25 (δH 1.34) and from H-3b (δH 2.53, d, J = 14.0 Hz) to H3-25 and H3-16 (δH 1.19), identified the relative configuration of 2 to be the same as that of 1. The similar Cotton effects in the ECD spectra ( Figure 4) suggested 2 to be a 15-dehydroxylated analogue of 1.   (Table 1) and analyses of the two dimensional nuclear magnetic resonance (2D NMR) data revealed that the structure of compound 2 was closely related to that of compound 1. The main difference was due to the presence of a methyl group (δ H 1.87, d, J = 6.8 Hz; δ C 18.1) at the side chain of ring C to replace the hydroxymethylene group of 1. The COSY relationship between H 3 -15 (δ H 1.87, d, J = 6.8 Hz) and H-14 (δ H 6.24, dq, J = 6.8, 15.0 Hz) in association with the HMBC correlations from H 3 -15 to C-13 (δ C 131.1) and C-14 (δ C 139.2) supported the methyl location. The similar NOE interactions, such as from H-9a (δ H 3.75) to H 3 -17 (δ H 1.45) and H 3 -25 (δ H 1.34) and from H-3b (δ H 2.53, d, J = 14.0 Hz) to H 3 -25 and H 3 -16 (δ H 1.19), identified the relative configuration of 2 to be the same as that of 1. The similar Cotton effects in the ECD spectra ( Figure 4) suggested 2 to be a 15-dehydroxylated analogue of 1. Mar   and NMR data, containing 11 degrees of unsaturation. The IR absorptions at 3429, 1730, and 1601 cm −1 suggested the presence of hydroxy and ketone groups. The 13 C NMR and DEPT spectra exhibited a total of 22 resonances for three methyls, one methylene, 11 methines, and seven quaternary carbons, of which 12 olefinic or aromatic resonances and two ketones were distinguished. A COSY spin system initiated from H-10 (δ H 6.30, d, J = 14.8 Hz) and extended to H-14 (δ H 1.94, d, J = 6.8 Hz), along with the HMBC correlation from H-10 and a D 2 O exchangeable proton at δ H 14.34 (s) to C-9 (δ C 167.1) and C-3 (δ C 112.0), assigned an enolic sorbyl side chain. A cyclohexandione ring was inferred from the HMBC correlations from H 3 -21 (δ H 0.93, s) to C-1 (δ C 64.6) and two ketone carbons C-2 (δ C 197.7) and C-6 (δ C 211.7), H 3 -22 (δ H 1.29, s) to C-4 (δ C 40.5), C-5 (δ C 74.8) and C-6, in addition to the correlation from H-4 (δ H 3.32, t, J = 2.8 Hz) to C-2, C-3, C-5, and C-6 ( Figure 2). These data also revealed the position of the methyl groups at C-1 and C-5 and an enolic sorbyl unit at C-3, respectively. The HMBC correlation from a D 2 O exchangeable proton at δ H 2.69 (s) to C-4, C-5, and C-6 showed C-5 to be hydroxylated. Moreover, a mono-substituted phenyl ring was recognized by the spin system of the aromatic protons at δ H 6.98 (d, J = 7.6 Hz, 2H) and 7.26 (t, J = 7.6 Hz, 3H). The remaining two alkyl carbons C-7 (δ C 47.7) and C-8 (δ C 31.4) formed a substituted ethyl unit according to the COSY relationship between H-7 (δ H 3.15, dd, J = 5.6, 10.8 Hz) and H 2 -8 (δ H 1.90, 3.05), while the linkage of the aromatic ring at C-7 was based on the HMBC correlation from H-7 to the aromatic carbons C-15 (δ C 141.4) and C-16/C-20 (δ C 128.4). Diagnostic COSY and HMBC data (Figure 2), such as the HMBC correlation between H 3 -21 and C-7 and the COSY relationship from H 2 -8 to H-4 and H-7, confirmed the fusion of an ethylbenzene unit to the cyclohexandione ring by the C-1/C-7 and C-4/C-8 linkage. Thus, the planar structure with a bicyclo (2,2,2) octane skeleton was established. The NOE interactions between H 3 -22/H-8b (δ H 3.05), H-8b/H-16(H-20) (δ H 6.98), and H-8b/ H-16(H-20) revealed Me-22 to be spatially approximated to H-8b, while both H-8b and the aromatic moiety were in the same face ( Figure 3). In order to assign the absolute configuration, the ECD data of eight isomers with the same planar structure of compound 3 were calculated at the B31YP/6-311++G(2d, 2p) level. As shown in Figure 5, isomers ( Figure 5A,C,E,G) with 1R and 4S configurations exhibited a negative Cotton effect (CE) at 300-340 nm and a positive CE at 350-400 nm, whereas the opposite phases were observed in the same range for the isomers with 1S and 4R configurations ( Figure 5B,D,F,H) based on the calculated results. Thus, the negative CE at 310 nm and positive CE at 350 nm in the ECD spectrum of 3 were in agreement with 1R and 4S configurations. However, the configurational variation at C-5 and C-7 slightly induced the Cotton effects. Based on the NOE correlation mentioned above, the aromatic moiety linked to C-7 was oriented toward the hydroxy-ketone bridge and OH-5 was located on the same side as the unsaturated side chain, thus the stereogenic centers at C-5 and C-7 were assumed to be 5R and 7S configurations.
carbons C-7 (δC 47.7) and C-8 (δC 31.4) formed a substituted ethyl unit according to the COSY relationship between H-7 (δH 3.15, dd, J = 5.6, 10.8 Hz) and H2-8 (δH 1.90, 3.05), while the linkage of the aromatic ring at C-7 was based on the HMBC correlation from H-7 to the aromatic carbons C-15 (δC 141.4) and C-16/C-20 (δC 128.4). Diagnostic COSY and HMBC data (Figure 2), such as the HMBC correlation between H3-21 and C-7 and the COSY relationship from H2-8 to H-4 and H-7, confirmed the fusion of an ethylbenzene unit to the cyclohexandione ring by the C-1/C-7 and C-4/C-8 linkage. Thus, the planar structure with a bicyclo (2,2,2) octane skeleton was established. The NOE interactions between H3-22/H-8b (δH 3.05), H-8b/H-16(H-20) (δH 6.98), and H-8b/ H-16(H-20) revealed Me-22 to be spatially approximated to H-8b, while both H-8b and the aromatic moiety were in the same face ( Figure 3). In order to assign the absolute configuration, the ECD data of eight isomers with the same planar structure of compound 3 were calculated at the B31YP/6-311++G(2d, 2p) level. As shown in Figure 5, isomers ( Figure 5A,C,E,G) with 1R and 4S configurations exhibited a negative Cotton effect (CE) at 300-340 nm and a positive CE at 350-400 nm, whereas the opposite phases were observed in the same range for the isomers with 1S and 4R configurations ( Figure  5B,D,F,H) based on the calculated results. Thus, the negative CE at 310 nm and positive CE at 350 nm in the ECD spectrum of 3 were in agreement with 1R and 4S configurations. However, the configurational variation at C-5 and C-7 slightly induced the Cotton effects. Based on the NOE correlation mentioned above, the aromatic moiety linked to C-7 was oriented toward the hydroxy-ketone bridge and OH-5 was located on the same side as the unsaturated side chain, thus the stereogenic centers at C-5 and C-7 were assumed to be 5R and 7S configurations. The NMR data of saturnispol D (4) were almost superimposed to those of 3 (Table 2), with the exception of a hydroxymethylene unit in 4 to replace the terminal methyl group at the enolic sorbyl side chain. The COSY relationship between the hydroxymethylene protons H2-14 (δH 4.28, d, J = 4.4 Hz) and the olefinic proton H-13 (δH 6.39, dt, J = 4.4, 15.2 Hz) in addition to the HMBC correlations from H2-14 to C-12 (δC 127.7) and C-13 (δC 142.9) indicated 4 to be a 14-hydroxylated analogue of 3. The comparable ECD data supported both 3 and 4 sharing the same configuration ( Figure 6). Table 2. 1 H and 13 C NMR data of 3-4 (δ (ppm), J (Hz)). The NMR data of saturnispol D (4) were almost superimposed to those of 3 (Table 2), with the exception of a hydroxymethylene unit in 4 to replace the terminal methyl group at the enolic sorbyl side chain. The COSY relationship between the hydroxymethylene protons H 2 -14 (δ H 4.28, d, J = 4.4 Hz) and the olefinic proton H-13 (δ H 6.39, dt, J = 4.4, 15.2 Hz) in addition to the HMBC correlations from H 2 -14 to C-12 (δ C 127.7) and C-13 (δ C 142.9) indicated 4 to be a 14-hydroxylated analogue of 3. The comparable ECD data supported both 3 and 4 sharing the same configuration ( Figure 6).   (Tables 3 and 4) suggested 5 to be structurally related to sorbicillinol [27]. A cyclohexenone nucleus was demonstrated by the COSY coupling between H-6 (δH 2.69, ddd, J = 4.8, 7.4, 12.0 Hz) and H2-5 (δH 1.66, 1.98), and the HMBC correlations from H3-13 (δH 1.34, s) to C-3 (δC 192.2), C-4 (δC 71.1), and C-5 (δC 37.1), H3-14 (δH 1.68, s) to C-1 (δC 183.2), C-2 (δC 108.4), and C-3, in association with the correlations from H-6 to C-1, C-2, C-4, and C-5. These findings also allowed the substitution of methyl groups at C-2 and C-4, respectively. The deshielded quaternary carbons C-3 and C-4, as in the case of 1, suggested both C-3 and C-4 to be hydroxylated. The COSY relationships established a spin system for a linear side chain of dihydroxyhexadiene, in which a conjugated diene was located between C-8 (δC 131.6) and C-11 (δC 133.5). The COSY relationships between H-7 (  (Tables 3 and 4) suggested 5 to be structurally related to sorbicillinol [27]. A cyclohexenone nucleus was demonstrated by the COSY coupling between H-6 (δ H 2.69, ddd, J = 4.8, 7.4, 12.0 Hz) and H 2 -5 (δ H 1.66, 1.98), and the HMBC correlations from H 3 -13 (δ H 1.34, s) to C-3 (δ C 192.2), C-4 (δ C 71.1), and C-5 (δ C 37.1), H 3 -14 (δ H 1.68, s) to C-1 (δ C 183.2), C-2 (δ C 108.4), and C-3, in association with the correlations from H-6 to C-1, C-2, C-4, and C-5. These findings also allowed the substitution of methyl groups at C-2 and C-4, respectively. The deshielded quaternary carbons C-3 and C-4, as in the case of 1, suggested both C-3 and C-4 to be hydroxylated. The COSY relationships established a spin system for a linear side chain of dihydroxyhexadiene, in which a conjugated diene was located between C-8 (δ C 131.6) and C-11 (δ C 133.5). The COSY relationships between H-7 (δ 2) and C-12 (δ C 61.7) as assigned by the HSQC spectrum, indicated both C-7 and C-12 to be hydroxylated. The linkage of the side chain to C-6 (δ C 43.9) was evident from the COSY coupling between H-6 and H-7, as well as the HMBC correlation from H-7 to C-1, C-5 and C-6. The E, E geometry of the conjugated diene was deduced by the coupling constants of J H-8/H-9 and J H-10/H-11 (15.4 Hz). The NOE interaction between H-6 and H 3 -13 suggested the same orientation of both protons (Figure 3). The J H-6/H-7 (7.4 Hz) value, in addition to the NOE interaction from H-7 (δ H 4.49) and H-8 to H-5a (δ H 1.66), and that between H-6 and H-8, suggested that the side chain maintained a stable geometry, in which H-7 was trans relative to H-6, while H-7 and H-8 were spatially close to H-5a. Thus, the relative configurations of 5 were suggested to be 4S*, 6S*, and 7S*. The ECD data for the stereogenic isomers of (4R, 6R, 7R)-5 and (4S, 6S, 7S)-5 were calculated. Comparison of the experimental ECD data with those calculated for the isomers confirmed 5 to be in 4S, 6S, and 7S configurations (Figure 7). (δC 61.7) as assigned by the HSQC spectrum, indicated both C-7 and C-12 to be hydroxylated. The linkage of the side chain to C-6 (δC 43.9) was evident from the COSY coupling between H-6 and H-7, as well as the HMBC correlation from H-7 to C-1, C-5 and C-6. The E, E geometry of the conjugated diene was deduced by the coupling constants of JH-8/H-9 and JH-10/H-11 (15.4 Hz). The NOE interaction between H-6 and H3-13 suggested the same orientation of both protons (Figure 3). The JH-6/H-7 (7.4 Hz) value, in addition to the NOE interaction from H-7 (δH 4.49) and H-8 to H-5a (δH 1.66), and that between H-6 and H-8, suggested that the side chain maintained a stable geometry, in which H-7 was trans relative to H-6, while H-7 and H-8 were spatially close to H-5a. Thus, the relative configurations of 5 were suggested to be 4S*, 6S*, and 7S*. The ECD data for the stereogenic isomers of (4R, 6R, 7R)-5 and (4S, 6S, 7S)-5 were calculated. Comparison of the experimental ECD data with those calculated for the isomers confirmed 5 to be in 4S, 6S, and 7S configurations (Figure 7).       Th remaining NMR resonances were attributed to C-1 (δ C 176.8), C-2 (δ C 93.6), C-3 (δ C 180.8), C-4 (δ C 83.0), and two methyl groups. The HMBC spectrum correlated H 3 -13 with C-3 and C-4 and H 3 -14 with C-1, C-2, and C-3, in association with the degrees of the molecular unsaturation, allowed the assignment of a 2,4-dimethyl-γ-lactone unit, while the remaining OH group was positioned at the quaternary carbon C-3. The linkage of the side chain to C-4 in the γ-lactone ring was evident from additional HMC correlations from H 3 -13 to C-5 (δ C 30.6) and H 2 -5 to C-3 and C-4. Thus, the planar structure of 6 was closely related to vertinolide [28], except for the substitution of a hydroxy group at C-12 (δ C 61.4). The E, E geometry of the diene was evidenced by the coupling constants of J H-8/H-9 and J H-10/H-11 (16.0 Hz). Regarding the absolute configuration, the comparable specific rotation between 6 ([α] 15 D , CHCl 3 ) and (S)-(-)-vertinolide [29] suggested the 4S configuration of 6. This depiction was confirmed by the experimental ECD data, which agreed with those calculated for the 4S isomer ( Figure 8).  to H2-5, H2-6, H-8, and H-9 (δH 7.25, dd, J = 10.6, 16.0 Hz), which established a 11-hydroxy-7-oxo-8,10-octadienyl side chain. The remaining NMR resonances were attributed to C-1 (δC 176.8), C-2 (δC 93.6), C-3 (δC 180.8), C-4 (δC 83.0), and two methyl groups. The HMBC spectrum correlated H3-13 with C-3 and C-4 and H3-14 with C-1, C-2, and C-3, in association with the degrees of the molecular unsaturation, allowed the assignment of a 2,4-dimethyl-γ-lactone unit, while the remaining OH group was positioned at the quaternary carbon C-3. The linkage of the side chain to C-4 in the γ-lactone ring was evident from additional HMC correlations from H3-13 to C-5 (δC 30.6) and H2-5 to C-3 and C-4. Thus, the planar structure of 6 was closely related to vertinolide [28], except for the substitution of a hydroxy group at C-12 (δC 61.4). The E, E geometry of the diene was evidenced by the coupling constants of JH-8/H-9 and JH-10/H-11 (16.0 Hz). Regarding the absolute configuration, the comparable specific rotation between 6 ([α] 15 D , CHCl3) and (S)-(-)-vertinolide [29] suggested the 4S configuration of 6. This depiction was confirmed by the experimental ECD data, which agreed with those calculated for the 4S isomer ( Figure 8). The HRESIMS data provided the molecular formula of saturnispol G (7) as C11H14O4, requiring 5 degrees of unsaturation. The HSQC spectrum assigned all protons and their corresponding carbons. The 1 H NMR spectrum showed three olefinic protons, a methyl singlet, and six alkyl protons, while the 13 C NMR and DEPT spectra afforded a methyl, three methylenes, six olefinic resonances, and a carbonyl carbon. Analyses of the HMBC data, such as the correlations from H3-11 The HRESIMS data provided the molecular formula of saturnispol G (7) as C 11 H 14 O 4 , requiring 5 degrees of unsaturation. The HSQC spectrum assigned all protons and their corresponding carbons. The 1 H NMR spectrum showed three olefinic protons, a methyl singlet, and six alkyl protons, while the 13 C NMR and DEPT spectra afforded a methyl, three methylenes, six olefinic resonances, and a carbonyl carbon. Analyses of the HMBC data, such as the correlations from H 3 -11 (δ H 1.89, s) to C-1 (δ C 164.4), C-2 (δ C 98.9), and C-3 (δ C 156.1) and from H-4 (δ H 6.01, s) to C-2 and C-5 (δ C 164.8), suggested a 2-methyl-3-hydroxypyranone nucleus. The COSY relationships provided a spin system for a hydroxylated pentene side chain, in which the hydroxy group was positioned at C-10 (δ C 60.7), based on the COSY relationship between H 2 -10 (δ H 3.61, t, J = 6.4 Hz) and H 2 -9 (δ H 1.71, tt, J = 6.4, 7.6 Hz). The HMBC correlation of the olefinic proton H-6 (δ H 6.11, d, J = 16.0 Hz) to C-4 (δ C 99.6) and C-5 (δ C 164.8) allowed the side chain to be linked to C-5. The 6E geometry was identified by the Biogenetically, sorbicillin-derived sorbicillinol is postulated as the precursor to generate the isolated compounds. The bisorbicillinoids 1 and 2 were supposed to be derived by the condensation of two sorbicillinol units via a Michael-type condensation, an approach similar to the biogenetic formation of bisvertinolone [30]. The hydroxylation at the terminal of the side chain was likely induced by the P450 enzyme. The (2 + 4) "Diels-Alder" cycloaddition [31] of sorbicillinol with a phenylethylene generated 3, followed by hydroxylation to yield 4. Compound 5 was likely generated from sorbicillinol by olefinic reduction and enol rearrangement. The unsaturated γ-lactone was supposed to be originated from sorbicillinol through reduction, hemiketal formation, retro-Claisen cleavage [32], and terminal hydroxylation. Neumann [33] postulated that the pyrone unit was derived from sorbicillinol by multiple steps. We suggest oxidation to lose a C 3 unit in sorbicillinol to produce 8 and 7 (Scheme 1). Biogenetically, sorbicillin-derived sorbicillinol is postulated as the precursor to generate the isolated compounds. The bisorbicillinoids 1 and 2 were supposed to be derived by the condensation of two sorbicillinol units via a Michael-type condensation, an approach similar to the biogenetic formation of bisvertinolone [30]. The hydroxylation at the terminal of the side chain was likely induced by the P450 enzyme. The (2 + 4) "Diels-Alder" cycloaddition [31] of sorbicillinol with a phenylethylene generated 3, followed by hydroxylation to yield 4. Compound 5 was likely generated from sorbicillinol by olefinic reduction and enol rearrangement. The unsaturated γ-lactone was supposed to be originated from sorbicillinol through reduction, hemiketal formation, retro-Claisen cleavage [32], and terminal hydroxylation. Neumann [33] postulated that the pyrone unit was derived from sorbicillinol by multiple steps. We suggest oxidation to lose a C3 unit in sorbicillinol to produce 8 and 7 (Scheme 1). Scheme 1. Postulation of the biogenetic relationship among the isolated compounds.

Mar. Drugs
All compounds were evaluated for antimicrobial activity toward several bacterial strains, including Staphylococcus aureus ATCC 29213, vancomycin-resistant Enterococci faecalis A4 (VRE), Bacillus subtilis ATCC 6051, Pseudomonas aeruginosa 14, and Klebsiella pneumoniae WNX-1. As shown in Table 5, the unsaturated γ-lactone 6 exhibited significant inhibition against a panel of bacterial strains, of which the MIC value toward VRE was 1.63 μg/mL. The pyrone derivative 8 selectively inhibited VRE and B. subtilis with an MIC value of 12.9 μg/mL. The remaining compounds showed MIC values higher than 64 μg/mL. All compounds were evaluated for antimicrobial activity toward several bacterial strains, including Staphylococcus aureus ATCC 29213, vancomycin-resistant Enterococci faecalis A4 (VRE), Bacillus subtilis ATCC 6051, Pseudomonas aeruginosa 14, and Klebsiella pneumoniae WNX-1. As shown in Table 5, the unsaturated γ-lactone 6 exhibited significant inhibition against a panel of bacterial strains, of which the MIC value toward VRE was 1.63 µg/mL. The pyrone derivative 8 selectively inhibited VRE and B. subtilis with an MIC value of 12.9 µg/mL. The remaining compounds showed MIC values higher than 64 µg/mL.

Discussion
Fungal species of the Trichoderma genus are recognized as biological control agents against plant-associated fungal pathogens, with the biological effects of these fungal strains due to the profiles of fungus-derived metabolites. Pentylpyrone from T. koningii inhibited the growth of several soilborne plant pathogens [15], while sorbicillin-related analogues exerted radical scavenging and other bioactivities [34]. These findings suggested that the Trichoderma fungus inhabiting the host sponges produces sorbicillinoids, which may play chemoecological role. In the present work, the fungus host, sponge Dictyonella incisa, was reported to produce conjugated and degraded sterols and fatty acids without cytotoxicity [35][36][37]. These data, along with the various bioactivities of sorbicillinoids, suggested that the endophytic fungus T. saturnisporum produced sorbicillinoid-based derivatives to help the host sponge in its defense against pathogenic invasion. For a pharmaceutical point of view, the pathogenic bacterium E. faecium is often resistant to vancomycin and ampicillin, and is difficult to treat by antibacterial agents [38]. The potent anti-VRE effect of the unsaturated γ-lactone (6) provides a new natural scaffold for further development as a promising anti-VRE lead after structural modification.
pneumoniae WNX-1 were supplied by Prof. Kui Zhu (the Microbiology Center, Agriculture University of China, Beijing, China).

Fungal Strain
The fungus, strain DI-IA, was isolated from the sponge Dictyonella incisa collected at a depth of 10 m in the Seferihisar bay of Turkey. It was identified as Trichoderma saturnisporum on the basis of its ribosomal internal transcribed spacers (ITS) and the 5.8S ribosomal RNA gene (ITS1-5.8S-ITS2). Working stocks were prepared on potato dextrose agar slants stored at 4 • C in our laboratory.

Fermentation, Extraction, and Isolation
The fermentation was carried out in Fernbach flasks (500 mL), which contained 80 g of rice. Distilled H 2 O (120 mL) was added to the flask, and the contents were soaked overnight before autoclaving at 15 psi for 30 min. After cooling to room temperature (rt), each flask was inoculated with 5.0 mL of the spore inoculums and incubated at 25 • C for 40 days. The fermented material was extracted successively with EtOAc (3 × 500 mL), and then evaporated to dryness under vacuum to afford a crude extract. The crude extracts were tested against S. aureus and showed an inhibitory effect with an MIC value of 64 µg/mL. Thus, the EtOAc extract was detected by the 1 H NMR spectrum, showing the rich conjugated olefinic resonances in the range of 5-7 ppm.

ECD Calculation
Conformational analyses were carried out via random searching in the Sybyl-X 2.0 using the MMFF94S force field with an energy cutoff of 2.5 kcal/mol. Subsequently, the conformers were re-optimized using discrete fourier transform (DFT) at the B3LYP/6-31++G (d) level in the gas phase using the GAUSSIAN 09 program. The energies, oscillator strengths, and rotational strengths (velocity) of the first 60 electronic excitations were calculated using the time-dependent density functional theory (TDDFT) methodology at the B3LYP/6-311++G (2d, 2p) level in vacuum. The ECD spectra were simulated by the overlapping Gaussian function (half the bandwidth at 1/e peak height, σ = 0.2 eV). To obtain the final spectra, the simulated spectra of the conformers for each structure were averaged according to the Boltzmann distribution theory and their relative Gibbs free energy (∆G). Theoretical ECD spectra of the corresponding enantiomers were obtained by the direct inversions of the ECD spectra of the abovementioned compounds.

Antibacterial Assay
All compounds were tested for antibacterial activities against Gram-positive bacteria, S. aureus (ATCC 29213), B. subtilis, and vancomycin-resistant E. faecalis A4; Gram-negative P. aeruginosa 14; and pathogenic bacterium K. pneumoniae. The minimum inhibitory concentrations (MIC) were determined using the broth micro-dilution method according to the clinical and laboratory standards institute (CLSI) 2015 guideline. The commercial antimicrobial agents Ciprofloxacin, Colistin, and Bacaucin were used as positive controls. After 16-20 h incubation at 37 • C, MIC values were defined as the lowest concentrations of antibiotics with no visible growth of bacteria. Values of more than 64 µg/mL were considered as no antibacterial activity.

Conclusions
Our work first explored the secondary metabolites of T. saturnisporum derived from a marine sponge, and continued to uncover eight new sorbicillin-based derivatives. This finding provided additional evidence to support the assumption that the same fungal species from a marine environment are capable of activating a distinct biosynthetic pathway, like those of terrestrial origin. Saturnispols A-H as the sorbicillin-based derivatives are classified into five different scaffolds, while a terminal hydroxylation at the side chain is rare in the sorbicillinold family. In the biogenetic consideration, these compounds were generated from sorbicillinol as a precursor to derive different skeletons. Compound 6 serves as a new natural scaffold that possesses significant inhibition against drug-resistant bacterium (VRE), suggesting it to be a potential lead to overcome certain drug-resistant pathogens after structural optimization. In addition, both K. pneumonia and P. aeruginosa are Gram-negative, indicating that they are resistant to a large range of antibiotics, resulting in clinically unsuccessful treatment. The significant inhibition of 6 against these two pathogens suggests that it may be a promising agent against Gram-negative bacteria.