Seaweed Extract (Stella Maris®) Activates Innate Immune Responses in Arabidopsis thaliana and Protects Host against Bacterial Pathogens

Insects and pathogenic infections (bacteria, viruses and fungi) cause huge losses in agriculturally important crops yearly. Due to the rise in pesticide and antibiotic resistance, our crops and livestock are increasingly at risk. There is a rising demand for environmentally friendly solutions to prevent crop decreases. Components of Ascophyllum nodosum seaweed extracts were recently found to boost plant immunity. The stimulatory activities of the A. nodosum marine alga-derived extract (Stella Maris®) were investigated in a broad range of immune assays. Elevated hydrogen peroxide production measured in a chemiluminescence assay suggested that the extract elicited a strong burst of reactive oxygen species. Arabidopsis seedlings treated with Stella Maris® activated the expression of WRKY30, CYP71A12 and PR-1 genes, the induction of which represent early, mid and late plant immune response, respectively. Finally, this study found that Stella Maris® inhibited the growth of multiple bacterial pathogens, including an opportunistic human pathogen that has demonstrated pathogenicity in plants. In summary, the pre-treatment with the seaweed extract protected Arabidopsis against subsequent infection by these pathogens.


Introduction
As the world's population is steadily increasing with no sign of stabilizing [1], a greater demand arises for a sustainable food supply [2]. With the impending threat of global warming and the rise of drug/pesticide resistant pests (bacteria, fungi, insects, etc.), our food sources are more vulnerable now than ever [3,4]. These problems affect many facets of food production including crops and livestock [4]. The use of synthetic pesticides on crop plants is declining due to reported side effects, which include air, soil and water contamination [5,6]. There is also pressure to reduce the use of antibiotics in the raising of farm animals, mainly due to increases in the number of antibiotic resistant human pathogens [7]. The use of genetically modified organisms (GMOs) is under debate due to the lack of knowledge about the safety of consuming these organisms [8,9]. Nevertheless, there are a number of GMO foods (mainly crop plants) approved for consumption within Canada and the United States [9]. There is also concern that GMO, when released into the environment may outcompete wild type organisms and transfer their transgenes by horizontal gene transfer [10]. Altogether, these factors underscore the need for agriand aquacultural communities to develop alternative natural products to protect crops and animals.
Plant immune systems differ from their mammalian counterparts [11]. Mammals have both an innate and adaptive immune system, whereas plants have only an innate immune system [11]. Plant pathogens

Stella Maris ® Activates Innate Immune Responses in Arabidopsis thaliana
To determine whether Arabidopsis thaliana elicited an immune response towards Stella Maris ® , the β-glucuronidase (GUS) histochemical assay was used. This assay was developed to investigate whether Arabidopsis roots respond to MAMPs/PAMPs, and if so, which cell types respond to such markers [35]. The GUS histochemical assay also allowed us to determine the location of the immune response [35]. In this case, the GUS reporter gene was put under the control of the pathogen-inducible CYP71A12 promoter (CYP71A12pro:GUS). CYP71A12 is inducible in the presence of immune elicitors such as bacterial flagellin, elongation factor Tu, and a bacterially secreted protease (protease IV from P. aeruginosa) [36]. All concentrations (0.05%, 0.5% and 5%) of Stella Maris ® elicited strong immune responses in the transgenic CYP71A12pro:GUS Arabidopsis plants (Figure 1b-d). There was no induction of the promoter in the stained negative control seedlings (Figure 1a).
Mar. Drugs 2018, 16, x FOR PEER REVIEW 3 of 12 defense-related gene measurement using reverse transcription and quantitative polymerase chain reaction (RT-qPCR). Importantly, it was found that Stella Maris ® can directly inhibit the growth of a diverse range of bacterial pathogens. Finally, it was shown that the Ascophyllum extract protected A. thaliana against the infection caused by these pathogens.

Stella Maris ® Activates Innate Immune Responses in Arabidopsis thaliana
To determine whether Arabidopsis thaliana elicited an immune response towards Stella Maris ® , the β-glucuronidase (GUS) histochemical assay was used. This assay was developed to investigate whether Arabidopsis roots respond to MAMPs/PAMPs, and if so, which cell types respond to such markers [35]. The GUS histochemical assay also allowed us to determine the location of the immune response [35]. In this case, the GUS reporter gene was put under the control of the pathogen-inducible CYP71A12 promoter (CYP71A12pro:GUS). CYP71A12 is inducible in the presence of immune elicitors such as bacterial flagellin, elongation factor Tu, and a bacterially secreted protease (protease IV from P. aeruginosa) [36]. All concentrations (0.05%, 0.5% and 5%) of Stella Maris ® elicited strong immune responses in the transgenic CYP71A12pro:GUS Arabidopsis plants (Figure 1b-d). There was no induction of the promoter in the stained negative control seedlings ( Figure 1a). Arabidopsis thaliana (CYP71A12) following a 6-h treatment with various concentrations of Stella Maris ® . Images were captured using the 20× objective lens. (a) Control seedling that was untreated. (b-d) were treated with 0.05%, 0.5% and 5%, respectively for 6 h prior to staining with GUS substrate solution. The experiments were repeated three times with the same result.
It was then determined whether the treatment of Arabidopsis with Ascophyllum extract activated other recognized plant defense responses. For example, reactive oxygen species (ROS) production in Arabidopsis is a response to invading pathogens. Specifically, hydrogen peroxide production can inhibit attacks from bacteria, viruses and fungi [37]. In addition, hydrogen peroxide production caused by pathogenic infections activates WRKY transcription factors, which in turn induce salicylic acid (SA) and jasmonic acid (JA) innate immunity pathways in Arabidopsis [38]. Therefore, ROS production was used as an indicator of a relatively upstream immune signaling event in Arabidopsis plants, and measured using a chemiluminescence assay. Maximum ROS production in Arabidopsis seedlings occurred after 9 min of incubation with Stella Maris ® and the ROS reaction mixture. A 0.05% concentration of the Ascophyllum extract produced the highest amount of ROS, which was approximately 4.2 fold greater than the background level in control plants (Figures 2 and S1). In contrast, Arabidopsis seedlings treated with higher concentrations of Ascophyllum extract did not in Arabidopsis thaliana (CYP71A12) following a 6-h treatment with various concentrations of Stella Maris ® . Images were captured using the 20× objective lens. (a) Control seedling that was untreated. (b-d) were treated with 0.05%, 0.5% and 5%, respectively for 6 h prior to staining with GUS substrate solution. The experiments were repeated three times with the same result.
It was then determined whether the treatment of Arabidopsis with Ascophyllum extract activated other recognized plant defense responses. For example, reactive oxygen species (ROS) production in Arabidopsis is a response to invading pathogens. Specifically, hydrogen peroxide production can inhibit attacks from bacteria, viruses and fungi [37]. In addition, hydrogen peroxide production caused by pathogenic infections activates WRKY transcription factors, which in turn induce salicylic acid (SA) and jasmonic acid (JA) innate immunity pathways in Arabidopsis [38]. Therefore, ROS production was used as an indicator of a relatively upstream immune signaling event in Arabidopsis plants, and measured using a chemiluminescence assay. Maximum ROS production in Arabidopsis seedlings occurred after 9 min of incubation with Stella Maris ® and the ROS reaction mixture. A 0.05% concentration of the Ascophyllum extract produced the highest amount of ROS, which was approximately 4.2 fold greater than the background level in control plants ( Figure 2 and Figure S1). In contrast, Arabidopsis seedlings treated with higher concentrations of Ascophyllum extract did not produce an obvious oxidative burst when compared to the control. This may be due to the nature of the assay and/or the colour of the extract ( Figure S2). The ROS assay is a chemiluminescence-based assay that relies on the production of light to measure the amount of hydrogen peroxide produced. Therefore, since the elicitor is a dark solution, the colour may interfere with the detection of light ( Figure S2). produce an obvious oxidative burst when compared to the control. This may be due to the nature of the assay and/or the colour of the extract ( Figure S2). The ROS assay is a chemiluminescence-based assay that relies on the production of light to measure the amount of hydrogen peroxide produced. Therefore, since the elicitor is a dark solution, the colour may interfere with the detection of light ( Figure S2). Plant immune response is tightly controlled by a cascade of signaling events. Previous studies have characterized how the immune signals are orchestrated by diverse immune regulators [12,13]. The transcriptomic responses in plants treated by MAMPs/PAMPs have been well characterized using RNA sequencing, coupled with RT-qPCR [36]. The mechanism by which Ascophyllum extract activates defense-related effectors was examined during various stages of the immune response using RT-qPCR to measure the relative gene expression that correlates to early, mid and late immune responses. The expression of WRKY30, CYP71A12 and PR-1 were measured at 1-, 6-and 24-h post treatment with Stella Maris ® , respectively. The RT-qPCR data indicate the relative gene expression of Arabidopsis seedlings treated with three concentrations of Ascophyllum extract compared to the buffertreated controls (Figure 3a-c). Arabidopsis seedlings treated with 5% extract showed the strongest immune activation for all genes measured (Figure 3a   Plant immune response is tightly controlled by a cascade of signaling events. Previous studies have characterized how the immune signals are orchestrated by diverse immune regulators [12,13]. The transcriptomic responses in plants treated by MAMPs/PAMPs have been well characterized using RNA sequencing, coupled with RT-qPCR [36]. The mechanism by which Ascophyllum extract activates defense-related effectors was examined during various stages of the immune response using RT-qPCR to measure the relative gene expression that correlates to early, mid and late immune responses. produce an obvious oxidative burst when compared to the control. This may be due to the nature of the assay and/or the colour of the extract ( Figure S2). The ROS assay is a chemiluminescence-based assay that relies on the production of light to measure the amount of hydrogen peroxide produced. Therefore, since the elicitor is a dark solution, the colour may interfere with the detection of light ( Figure S2). Plant immune response is tightly controlled by a cascade of signaling events. Previous studies have characterized how the immune signals are orchestrated by diverse immune regulators [12,13]. The transcriptomic responses in plants treated by MAMPs/PAMPs have been well characterized using RNA sequencing, coupled with RT-qPCR [36]. The mechanism by which Ascophyllum extract activates defense-related effectors was examined during various stages of the immune response using RT-qPCR to measure the relative gene expression that correlates to early, mid and late immune responses. The expression of WRKY30, CYP71A12 and PR-1 were measured at 1-, 6-and 24-h post treatment with Stella Maris ® , respectively. The RT-qPCR data indicate the relative gene expression of Arabidopsis seedlings treated with three concentrations of Ascophyllum extract compared to the buffertreated controls (Figure 3a-c). Arabidopsis seedlings treated with 5% extract showed the strongest immune activation for all genes measured (Figure 3a

Stella Maris ® Inhibits the Growth of Multiple Bacteria
Ascophyllum extract-triggered immune response was examined to identify whether it could help protect against pathogenic infections. Before evaluating whether the plant defense response boosted by the extract could prime their immunity to fend off pathogens, the ability of the Ascophyllum extract to directly inhibit the growth of bacterial pathogens was investigated. The direct anti-microbial activity of Stella Maris ® has not been documented previously. The number of colony forming units (CFU) was measured to assess whether Stella Maris ® inhibited the growth of the well-characterized plant pathogen P. syringae DC3000. In the presence of 4%, 20% and 50% concentrations of Stella Maris ® in LB broth, the overnight growth of P. syringae was reduced 35%, 45% and 58%, respectively, compared to the control (LB alone) (Figure 4a). There were no significant differences in growth between the control and the 1% and 2% concentrations of Stella Maris ® in LB broth (Figure 4a). The extract also inhibited the growth of a broad-host-range opportunistic pathogen Pseudomonas aeruginosa. However, much higher concentrations, 20% and 50%, of Stella Maris ® were needed to inhibit P. aeruginosa by 93% and 97%, respectively (Figure 4b). There was no significant difference in growth between the control and 1%, 2% and 4% concentrations of Stella Maris ® in LB broth (Figure 4b). Finally, the inhibition noted in Pseudomonas was tested for applicability to another genus of plant pathogens. Incubation of Stella Maris ® at concentrations of 4%, 20% and 50% in NYG broth inhibited the growth of Xanthomonas campestris by 31%, 36% and 43%, respectively (Figure 4c). No significant difference was observed in growth between the control and 1% and 2% concentrations of Stella Maris ® in NYG broth (Figure 4b).

Stella Maris ® Inhibits the Growth of Multiple Bacteria
Ascophyllum extract-triggered immune response was examined to identify whether it could help protect against pathogenic infections. Before evaluating whether the plant defense response boosted by the extract could prime their immunity to fend off pathogens, the ability of the Ascophyllum extract to directly inhibit the growth of bacterial pathogens was investigated. The direct anti-microbial activity of Stella Maris ® has not been documented previously. The number of colony forming units (CFU) was measured to assess whether Stella Maris ® inhibited the growth of the well-characterized plant pathogen P. syringae DC3000. In the presence of 4%, 20% and 50% concentrations of Stella Maris ® in LB broth, the overnight growth of P. syringae was reduced 35%, 45% and 58%, respectively, compared to the control (LB alone) (Figure 4a). There were no significant differences in growth between the control and the 1% and 2% concentrations of Stella Maris ® in LB broth (Figure 4a). The extract also inhibited the growth of a broad-host-range opportunistic pathogen Pseudomonas aeruginosa. However, much higher concentrations, 20% and 50%, of Stella Maris ® were needed to inhibit P. aeruginosa by 93% and 97%, respectively (Figure 4b). There was no significant difference in growth between the control and 1%, 2% and 4% concentrations of Stella Maris ® in LB broth (Figure 4b).
Finally, the inhibition noted in Pseudomonas was tested for applicability to another genus of plant pathogens. Incubation of Stella Maris ® at concentrations of 4%, 20% and 50% in NYG broth inhibited the growth of Xanthomonas campestris by 31%, 36% and 43%, respectively (Figure 4c). No significant difference was observed in growth between the control and 1% and 2% concentrations of Stella Maris ® in NYG broth (Figure 4b).

Stella Maris ® Protects Arabidopsis thaliana against Bacterial Pathogens
Based on the data above, the protection assay was performed as follows. At higher concentrations (>4% as shown in Figure 4), Stella Maris ® demonstrated a direct inhibitory effect on bacterial growth. Therefore, concentrations that didn't show a detrimental outcome on bacterial growth were chosen. However, they were still effective at eliciting a robust immune response and were tested in a pretreatment experiment. To avoid potential complications from the presence of the Ascophyllum extract on bacterial growth, it was removed from the plant growth media after boosting plant immune response and treated plants were washed several times with plant growth medium before inoculating with the bacterial pathogens. The CFU's of P. syringae, P. aeruginosa and X. campestris were measured to determine whether pretreatment with Stella Maris ® provided protection. With regard to bacterial load in the MS media (exterior to the plant), 1% Stella Maris ® provided the greatest level of induction of protection against all three bacterial pathogens at all times measured (Figures 5a-c and S3a-c). Furthermore, it reduced bacterial load at 24 h by 20%, 28% and 41% in plants infected

Stella Maris ® Protects Arabidopsis thaliana against Bacterial Pathogens
Based on the data above, the protection assay was performed as follows. At higher concentrations (>4% as shown in Figure 4), Stella Maris ® demonstrated a direct inhibitory effect on bacterial growth. Therefore, concentrations that didn't show a detrimental outcome on bacterial growth were chosen. However, they were still effective at eliciting a robust immune response and were tested in a pretreatment experiment. To avoid potential complications from the presence of the Ascophyllum extract on bacterial growth, it was removed from the plant growth media after boosting plant immune response and treated plants were washed several times with plant growth medium before inoculating with the bacterial pathogens. The CFU's of P. syringae, P. aeruginosa and X. campestris were measured to determine whether pretreatment with Stella Maris ® provided protection. With regard to bacterial load in the MS media (exterior to the plant), 1% Stella Maris ® provided the greatest level of induction of protection against all three bacterial pathogens at all times measured (Figure 5a-c and Figure  S3a-c). Furthermore, it reduced bacterial load at 24 h by 20%, 28% and 41% in plants infected by P. syringae, P. aeruginosa and X. campestris, respectively (Figure 5a-c). Stella Maris ® at a concentration of 0.2% also induced protection against the bacterial pathogens, however to a lesser extent (10%, 14% and 28%, respectively) (Figure 5a-c). In all cases, no induced protection was observed against pathogens treated with 0.1% of Stella Maris ® (Figure 5a-c). Pretreatment with 0.1%, 0.2% and 1% concentrations of Stella Maris ® also reduced P. syringae bacterial load within Arabidopsis tissue by 30%, 31% and 47%, respectively (Figure 5d). Arabidopsis pretreated with Stella Maris ® at a concentrations of 1% reduced P. aeruginosa bacterial burden within the plant tissues by 22% relative to the control (Figure 5e). However, no significant protection was afforded against X. campestris bacterial burden within Arabidopsis tissues (Figure 5f).
Mar. Drugs 2018, 16, x FOR PEER REVIEW 6 of 12 by P. syringae, P. aeruginosa and X. campestris, respectively (Figure 5a-c). Stella Maris ® at a concentration of 0.2% also induced protection against the bacterial pathogens, however to a lesser extent (10%, 14% and 28%, respectively) (Figure 5a-c). In all cases, no induced protection was observed against pathogens treated with 0.1% of Stella Maris ® (Figure 5a-c). Pretreatment with 0.1%, 0.2% and 1% concentrations of Stella Maris ® also reduced P. syringae bacterial load within Arabidopsis tissue by 30%, 31% and 47%, respectively (Figure 5d). Arabidopsis pretreated with Stella Maris ® at a concentrations of 1% reduced P. aeruginosa bacterial burden within the plant tissues by 22% relative to the control (Figure 5e). However, no significant protection was afforded against X. campestris bacterial burden within Arabidopsis tissues (Figure 5f).

Discussion
With the increase in pesticide resistance among many plant pathogens, and harmful environmental effects found with these pesticides, there is a need for safe biologically active compounds that can boost plant immune response and inhibit the growth of pathogenic fungi and bacteria [5]. Stella Maris ® is a commercially available Ascophyllum extract with known PGP capabilities [39]. However, there is little current evidence that this extract can boost plant immunity. Seaweed extracts have been fractioned into biologically active compounds such as ulvans and oligoulvans, oligo-alginate, fucans and oligo-fucans, laminarin and carrageenans and oligo-carrageenans [29]. However, studies have not looked at seaweed extracts as a whole, and in many cases the individual biological components of the extracts only activated one plant immune response pathway [30][31][32][33][34]. By treating plants with complete seaweed extract, it is hypothesized to be more beneficial to the plant by activating several pathways within the innate immune response.
Stella Maris ® immune modulating abilities were assessed in A. thaliana, and our results showed that the extract activated a strong innate immune response in Arabidopsis as evidenced by wellcharacterized high-throughput assays. The ROS chemiluminescence assay showed that the

Discussion
With the increase in pesticide resistance among many plant pathogens, and harmful environmental effects found with these pesticides, there is a need for safe biologically active compounds that can boost plant immune response and inhibit the growth of pathogenic fungi and bacteria [5]. Stella Maris ® is a commercially available Ascophyllum extract with known PGP capabilities [39]. However, there is little current evidence that this extract can boost plant immunity. Seaweed extracts have been fractioned into biologically active compounds such as ulvans and oligo-ulvans, oligo-alginate, fucans and oligo-fucans, laminarin and carrageenans and oligo-carrageenans [29]. However, studies have not looked at seaweed extracts as a whole, and in many cases the individual biological components of the extracts only activated one plant immune response pathway [30][31][32][33][34]. By treating plants with complete seaweed extract, it is hypothesized to be more beneficial to the plant by activating several pathways within the innate immune response.
Stella Maris ® immune modulating abilities were assessed in A. thaliana, and our results showed that the extract activated a strong innate immune response in Arabidopsis as evidenced by well-characterized high-throughput assays. The ROS chemiluminescence assay showed that the Ascophyllum extract activates a rapid (9 min post-treatment) immune response in the form of hydrogen peroxide production ( Figure 2). Plants treated with lower concentrations (0.05%) of this extract produced more ROS compared to higher concentrations (0.2% and 0.5%), a result most likely due to the fact that the Ascophyllum extract is naturally dark brown. Therefore, higher concentrations are less transparent making it more difficult for light to be detected by the plate reader. It is suggested that if all samples had similar transparency, a ROS response from the higher concentrations of the Ascophyllum extract would have been detected. Nonetheless, the extract was shown to be capable of eliciting a strong immune response in the form of hydrogen peroxide production. Arabidopsis seedlings treated with the Ascophyllum extract (0.05%) produced 0.8-fold more hydrogen peroxide compared to plants treated with 100 nM of flg22 ( Figure S1). This is highly significant given that flg22 is a synthetic flagellar peptide, well known to produce a very strong immune response in Arabidopsis [40].
Subsequently, it was found that the Ascophyllum extract activated the expression of a transcription factor, WRKY30, which responds to hydrogen peroxide production and is involved in the expression of genes within the SA and JA immunity signaling pathways [41]. SA and JA are important plant defense hormones that have been shown to deter pathogens [42]. SA is more effective against biotrophic (living plant tissues) pathogens, whereas JA is better deterrent for necrotrophic (cell-death provoking) pathogens [42].
The Ascophyllum extract also elicited a strong immune response in Arabidopsis seedlings 6 h post-treatment as indicated by the expression of CYP71A12. It was reported that CYP71A12 expression is maximally induced at the 6-h time point [36]. Using CYP71A12pro:GUS reporter transgenic plants, the activation of an immune response by Ascophyllum extract was found in the elongation zone of the root tip. Further, an increase in the expression of CYP71A12 in the seedlings treated with the Ascophyllum extract was observed relative to the untreated control using RT-qPCR. CYP71A12 is a cytochrome P450 family polypeptide responsible for the production of camalexin, a phytoalexin with antimicrobial functions that damage cell walls and disrupt metabolism in attacking pathogens [43]. For this reason, phytoalexins are known to accumulate in areas of infection [43]. A similar immune activation pattern is seen when the immune response is elicited with bacterial flagellin in Arabidopsis [35,36].
Finally, Stella Maris ® was examined for its ability to sustain an immune response. The expression of PR-1, a defense gene regulated by SA production involved in systemic acquired resistance to attacking pathogens, was measured [44]. The PR-1 gene expression was experimentally determined to be maximally induced by bacterial flagellin at the 24-h time point [45]. Consistently, the 24-h time point showed a strong activation of PR-1 by the Ascophyllum extract in Arabidopsis seedlings, therefore we chose to examine the protective capabilities of Stella Maris ® in Arabidopsis when challenged with diverse bacterial pathogens. A decrease in the number of bacteria found free-living in the MS media was observed as well as a decrease in bacteria found within the seedlings were pre-treated with the Ascophyllum extract for 24 h. The decrease in bacteria free-living within the MS media is likely due to the production of hydrogen peroxide and other antimicrobial compounds by the Arabidopsis seedlings, whereas the decrease in bacteria found within the plant tissue may be due to strengthening of the plant cell wall [45]. It was important for the plant protection assay that the Stella Maris ® be washed away prior to bacterial infection because the extract also has a noticeable inhibitory effect on bacterial growth.

Plant Growth
A. thaliana seeds were surface sterilized using equal parts of bleach, sterile water and 70% ethanol for 5 min, then washed three times using sterile water and stored at 4 • C for two days prior to planting. Seedlings were grown in MS liquid media (Murashige and Skoog basal medium with vitamins from Phytotechnology Laboratories supplemented with 0.5% sucrose and 0.5 g L −1 MES hydrate buffered to pH 5.7 using 1N potassium hydroxide, Lenexa, KA, USA) in either 24-well plates (BD Falcon, Bedford, MA, USA) (ten seeds and 0.5 mL medium per well) for GUS expression, RT-qPCR and protection assays, or 96-well plates (Greiner Bio-One, Kremsmünster, Austria) (one seed and 0.2 mL medium per well) for oxidative burst measurements. In both cases plates were sealed with Micropore tape and placed on a grid-shelve over water on a growth light stand (Hydrofarms, Fairless Hills, PA, USA) for 10 days at 22 • C, under 16 h of daylight (100 µE m −2 s −1 ). The media in the 24-well plates was exchanged for fresh media on day 8, while the media in the 96-well plate was exchanged for sterile water on day 9.

Elicitor Treatments
Optimal concentrations of Stella Maris ® , an extract of the brown seaweed Ascophyllum nodosum, were experimentally determined to be 0.05%, 0.5% and 5% in sterile water for GUS expression, ROS measurement and RT-qPCR, and 0.1%, 0.2% and 1% for the protection assay. Ten-day-old seedlings were treated with the different elicitors for the following times: 6 h for GUS assay in reporter line CYP71A12pro:GUS: 1 h, 6 h and 24 h for RT-qPCR analysis of selected genes. Nine-day-old seedlings were treated with the different elicitors for 24 h prior to the protection assay.

Oxidative Burst Measurement
H 2 O 2 production in plants was detected using a luminol-horse radish peroxidase (HRP)-based chemiluminescence assay. A 10 mg mL −1 of 500× HRP (Sigma-Aldrich, St. Louis, MO, USA) stock solution was prepared by dissolving 10 mg HRP in sterile water. A 20 mg mL −1 of 500× luminol (Sigma-Aldrich, St. Louis, MO,USA) stock solution was prepared by dissolving 20 mg luminol in 100 mM KOH. For each elicitor concentration of Stella Maris ® , a master reaction mixture was prepared by diluting the elicitor in HRP, and luminol stock solutions in sterile water. The 96 well plates containing the seedlings were kept in the dark for 1 h prior to the treatment with each respective elicitor. The following procedures were completed in a dark room. Water was removed from each well within the 96 well plate following the 1 h pretreatment in the dark, and 200 µL of elicitor master reaction mixture was added to each well. The plate was then placed in a 96 well plate reader VICTOR™X5 (PerkinElmer, Waltham, MA, USA), and read for 30 cycles. The kinetics of H 2 O 2 production was determined by plotting the average of chemiluminescence counts from all the seedlings placed under the same elicitor treatment over the measurement period.

Bacterial Growth
Pseudomonas syringae DC3000 and Xanthomonas campestris BP109 were grown overnight for 16 h at 30 • C. Pseudomonas aeruginosa PA14 was grown overnight for 16 h at 37 • C. Overnight cultures were centrifuged at 5000× g for 10 min, washed twice with 10 mM MgSO 4 . The pathogens were resuspended separately in MS liquid media for the protection assay. P. syringae DC3000 and P. aeruginosa PA14 were resuspended in LB broth for growth inhibition assays. X. campestris was resuspended in NYG (5 g of peptone, 3 g of yeast extract, and 20 g of glycerol per litre) broth for the growth inhibition assay. Bacterial optical densities were adjusted to 0.0002 and 0.01 for the protection and growth inhibition assays, respectively.

Growth Inhibition Assay
Bacterial cultures were grown overnight for 16 h in various concentrations of Stella Maris ® (1%, 2%, 4%, 20% and 50%) in either LB broth for P. syringae DC3000 and P. aeruginosa PA14 or NYG broth for X. campestris BP109, then serially diluted from 10 0 -10 −7 . The plates were grown at room temperature (22 • C) overnight, and CFU were counted the following day.

Protection Assay
The elicitors were removed on day 10 of the assay, by washing the plants three times using sterile water, before adding either P. syringae DC3000, P. aeruginosa PA14 or X. campestris BP109 to each well. Liquid media samples were taken from each well at 16 h, 20 h, and 24 h. The liquid media were serially diluted from 10 0 -10 −7 , and plated on LB plates. The plants within each well were collected at 28 h post-infection, and their tissues were homogenized and plated on LB or NYG plates. The plates were incubated at room temperature (22 • C) overnight, and CFU were counted the following day.

Conclusions
The Ascophyllum nodosum extract, Stella Maris ® , was found to modulate early-, mid-and late-immune responses in the model organism Arabidopsis. The extract not only directly inhibits the growth of all three bacterial pathogens tested, including an opportunistic human pathogen, it also activates a strong immune response in plants that had a protective effect against these bacterial pathogens. This study suggests that seaweed extracts are effective biofertilizers due to the activation of the innate immune response in plants. Moving forward, seaweed extracts are an environmentally friendly option to enhance crop yield.