A Novel Bromophenol Derivative BOS-102 Induces Cell Cycle Arrest and Apoptosis in Human A549 Lung Cancer Cells via ROS-Mediated PI3K/Akt and the MAPK Signaling Pathway

Bromophenol is a type of natural marine product. It has excellent biological activities, especially anticancer activities. In our study of searching for potent anticancer drugs, a novel bromophenol derivative containing indolin-2-one moiety, 3-(4-(3-([1,4′-bipiperidin]-1′-yl)propoxy)-3-bromo-5-methoxybenzylidene)-N-(4-bromophenyl)-2-oxoindoline-5-sulfonamide (BOS-102) was synthesized, which showed excellent anticancer activities on human lung cancer cell lines. A study of the mechanisms indicated that BOS-102 could significantly block cell proliferation in human A549 lung cancer cells and effectively induce G0/G1 cell cycle arrest via targeting cyclin D1 and cyclin-dependent kinase 4 (CDK4). BOS-102 could also induce apoptosis, including activating caspase-3 and poly (ADP-ribose) polymerase (PARP), increasing the Bax/Bcl-2 ratio, enhancing reactive oxygen species (ROS) generation, decreasing mitochondrial membrane potential (MMP, ΔΨm), and leading cytochrome c release from mitochondria. Further research revealed that BOS-102 deactivated the PI3K/Akt pathway and activated the mitogen-activated protein kinase (MAPK) signaling pathway resulting in apoptosis and cell cycle arrest, which indicated that BOS-102 has the potential to develop into an anticancer drug.


Introduction
In China, cancer morbidity and mortality are increasing year by year. It has become the leading cause of death and seriously affected the quality of life of people with cancer [1]. Lung cancer is the cancer with the highest mortality rate in the world [1][2][3]. Among lung cancers, approximately 85% of cases are non-small cell lung cancer (NSCLC). Thus, it is highly desirable to develop safe and effective drugs to treat NSCLC and to improve the quality of life of patients with lung cancer.
Apoptosis can occur by one of two representative pathways: the mitochondrial pathway and the death receptor-mediated pathway [4]. In the mitochondrial pathway, ROS plays an important Apoptosis can occur by one of two representative pathways: the mitochondrial pathway and the death receptor-mediated pathway [4]. In the mitochondrial pathway, ROS plays an important role [5]. Generated ROS directly activates the mitochondrial permeability transition and results in mitochondrial membrane potential (MMP, ΔΨm) loss, causing the release of cytochrome c. Then the released cytochrome c activates caspases, and the activated caspases eventually lead to apoptosis [6][7][8]. In addition, ROS could also induce other various signaling pathways, such as the PI3K/Akt signaling pathway, which plays an important role in cell proliferation and survival [9]. When cells are subject to external stimuli, such as anticancer drug stimulation, the PI3K/Akt signaling pathway may be down-regulated, and finally induce cell apoptosis. The PI3K/Akt pathway also plays an important role in cell cycle regulation; the mechanism of this pathway is the regulation of CDK4 and cyclin D1, which could block cell cycle in G1 phase [10].
ROS is a second messenger in cells, and plays an important role in cell proliferation or apoptosis [11]. It has been demonstrated that ROS generation in cells could activate mitogen-activated protein kinase (MAPK) signaling pathways including p38 MAPK and ERK1/2 [12]. MAPK pathways are key mediators of eukaryotic transcription and they control gene expression via phosphorylation [13].
Bromophenols, an important kind of natural marine product, extracted from a variety of marine organisms, exhibit excellent biological activities, such as antitumor and antibacterial activities [14][15][16]. However, the natural bromophenol in the marine organisms is in low content, and its separation is difficult. Additionally, there are more or less deficiencies in its efficacy, stability, biological toxicity, and bioavailability. These are extremely limited for its further research and development [15].
Synthesis of new bromophenol compounds, or modifications to the structure of natural bromophenols, can effectively improve the biological activity of bromophenol compounds. Based on this foundation, a series of bromophenol compounds with potent anticancer activities were designed and synthesized in our previous works [14]. These compounds showed anticancer activity on human cancer cell lines, such as the human lung cancer cell line, human hepatoma cell lines, the human cervical cancer cell line, and the human colon cancer cell line. Among of them, a novel bromophenol derivative containing indolin-2-one moiety, 3-(4-(3-([1,4′-bipiperidin]-1′-yl)propoxy)-3-bromo-5methoxybenzylidene)-N-(4-bromophenyl)-2-oxoindoline-5-sulfonamide (BOS-102, Figure 1) showed excellent anticancer activities on human lung cancer cell lines [17]. In order to study whether compound BOS-102 has the potential to develop into an anticancer drug, more experiments regarding the molecular mechanisms, including cell apoptosis analysis, cell cycle analysis, ROS generation analysis, mitochondrial membrane potential analysis, and potential signaling pathways (PI3K/Akt and MAPK) analysis in A549 lung cancer cells were explored in this study.

BOS-102 Inhibits Colony Formation
A colony formation assay was performed to study the effect of BOS-102 on cell colony formation. A549 cells were seeded in six-well plates at a density of 500 cells per well. After 24 h, cells were treated with BOS-102 (0, 2.5, 5, 10 µM), and incubated for 10 days. In our study, the results showed that BOS-102 can significantly inhibit the colony formation of A549 cells ( Figure 2B,C).

BOS-102 Inhibits Colony Formation
A colony formation assay was performed to study the effect of BOS-102 on cell colony formation. A549 cells were seeded in six-well plates at a density of 500 cells per well. After 24 h, cells were treated with BOS-102 (0, 2.5, 5, 10 µM), and incubated for 10 days. In our study, the results showed that BOS-102 can significantly inhibit the colony formation of A549 cells ( Figure 2B,C).
Apoptosis often causes cell morphological changes, such as nuclear apoptotic bodies [18]. It is interesting to investigate the effect of BOS-102 apoptosis induction by Hoechst 33258 staining in the A549 cell line. A549 cells were treated with BOS-102 (0, 2.5, 5, 10 µM) for 48 h. As shown in Figure 3C, after staining with Hoechst 33258, cell nuclear condensation, chromosome condensation, and apoptotic bodies were observed in BOS-102-treated cells.
Apoptosis often causes cell morphological changes, such as nuclear apoptotic bodies [18]. It is interesting to investigate the effect of BOS-102 apoptosis induction by Hoechst 33258 staining in the A549 cell line. A549 cells were treated with BOS-102 (0, 2.5, 5, 10 µM) for 48 h. As shown in Figure 3C, after staining with Hoechst 33258, cell nuclear condensation, chromosome condensation, and apoptotic bodies were observed in BOS-102-treated cells.

Effect of BOS-102 on the Expression of Apoptosis-Related Proteins
When apoptosis occurred, the expression of apoptosis related proteins, such as Bax, Bcl-2, caspase-3, and PARP may change. Western blot was used to detect the expression of these proteins. After treatment with BOS-102 for 48 h, the expression of Bax was increased while the Bcl-2 was decreased ( Figure 3F). Furthermore, caspase-3 and PARP were also activated after BOS-102 treatment ( Figure 3F). Our results indicated that BOS-102 induced apoptosis on A549 cells probably through the mitochondrial-mediated apoptotic pathway.

Effect of BOS-102 on the Expression of Apoptosis-Related Proteins
When apoptosis occurred, the expression of apoptosis related proteins, such as Bax, Bcl-2, caspase-3, and PARP may change. Western blot was used to detect the expression of these proteins. After treatment with BOS-102 for 48 h, the expression of Bax was increased while the Bcl-2 was decreased ( Figure 3F). Furthermore, caspase-3 and PARP were also activated after BOS-102 treatment ( Figure 3F). Our results indicated that BOS-102 induced apoptosis on A549 cells probably through the mitochondrial-mediated apoptotic pathway.
To explore the mechanism for BOS-102-induced cell cycle arrest, Western blot analysis was used to examine the effect of BOS-102 treatment on the expression levels of cell cycle-related proteins, such as cyclin D1 and CDK4. In our study, BOS-102 treatment caused a significant decrease in the protein levels of cyclin D1 and CDK4 ( Figure 4C).
To explore the mechanism for BOS-102-induced cell cycle arrest, Western blot analysis was used to examine the effect of BOS-102 treatment on the expression levels of cell cycle-related proteins, such as cyclin D1 and CDK4. In our study, BOS-102 treatment caused a significant decrease in the protein levels of cyclin D1 and CDK4 ( Figure 4C).

BOS-102 Induces Mitochondrial Dysfunctionin in A549 Cells
The maintenance of MMP (∆Ψm) is significant for mitochondrial integrity and bioenergetic function [19]. The decline of MMP is a marker of apoptosis. We used JC-1 to detect the decline of MMP of A549 cells after BOS-102 treatment. In this study, the results indicated that BOS-102 significantly reduced the MMP in A549 cells compared with the control group ( Figure 5E,F). Additionally, we detected the expression of cytochrome c in mitochondria and cytoplasm using Western blot analysis. The results indicated that BOS-102 decreased cytochrome c in mitochondria, while increased it in cytoplasm ( Figure 5G). The results indicated that BOS-102 induced apoptosis in A549 cells through the mitochondrial apoptotic pathway.

BOS-102 Induces ROS Generation in A549 Cells
ROS generation is considered as one of the key mediators of apoptotic signaling. We used DCFH-DA to detect ROS in A549 cells. In our study, a rapid production of ROS was occurred after the treatment of BOS-102 (0, 2.5, 5, 10 µM) for 48 h. The result showed that DCFH-DA fluorescence intensity was increased in a dose-dependent manner. Compared with control group, the content of ROS was increased to 185.84 ± 24.91%, 211.50 ± 7.69%, and 233.36 ± 18.52% ( Figure 5A-C). Next, A549 cells were treated with 5 µM BOS-102 combined with/without 5 mM N-acetyl cysteine (NAC) for 48

BOS-102 Induces Mitochondrial Dysfunctionin in A549 Cells
The maintenance of MMP (∆Ψ m ) is significant for mitochondrial integrity and bioenergetic function [19]. The decline of MMP is a marker of apoptosis. We used JC-1 to detect the decline of MMP of A549 cells after BOS-102 treatment. In this study, the results indicated that BOS-102 significantly reduced the MMP in A549 cells compared with the control group ( Figure 5E,F). Additionally, we detected the expression of cytochrome c in mitochondria and cytoplasm using Western blot analysis. The results indicated that BOS-102 decreased cytochrome c in mitochondria, while increased it in cytoplasm ( Figure 5G). The results indicated that BOS-102 induced apoptosis in A549 cells through the mitochondrial apoptotic pathway.

BOS-102 Induces ROS Generation in A549 Cells
ROS generation is considered as one of the key mediators of apoptotic signaling. We used DCFH-DA to detect ROS in A549 cells. In our study, a rapid production of ROS was occurred after the treatment of BOS-102 (0, 2.5, 5, 10 µM) for 48 h. The result showed that DCFH-DA fluorescence intensity was increased in a dose-dependent manner. Compared with control group, the content of ROS was increased to 185.84 ± 24.91%, 211.50 ± 7.69%, and 233.36 ± 18.52% ( Figure 5A-C). Next, A549 cells were treated with 5 µM BOS-102 combined with/without 5 mM N-acetyl cysteine (NAC) for 48 h, respectively. Interestingly, we observed that the ROS inhibitor NAC blocked ROS generation in A549 cells ( Figure 5D). We also observed that NAC blocked BOS-102-induced apoptosis in A549 cells (data not shown). These results indicated that ROS generation is important in BOS-102-induced apoptosis.
Mar. Drugs 2017, 15, x FOR PEER REVIEW 6 of 13 h, respectively. Interestingly, we observed that the ROS inhibitor NAC blocked ROS generation in A549 cells ( Figure 5D). We also observed that NAC blocked BOS-102-induced apoptosis in A549 cells (data not shown). These results indicated that ROS generation is important in BOS-102-induced apoptosis.

BOS-102 Suppresses the PI3K/Akt Signaling Pathway
When apoptosis occurs, the PI3K/Akt signaling pathway plays an important role, which is also an important modulator in ROS-related cell apoptosis. In our study, we used Western blot analysis to detect the expression levels of PI3K/Akt after treatment of BOS-102. As shown in Figure 6A, the phosphorylation of Akt and PI3K were decreased in a dose-dependent manner after BOS-102 treatment. Furthermore, pretreatment of antioxidant NAC, a ROS inhibitor, could efficiently reverse the BOS-102-induced Akt phosphorylation ( Figure 6B). These results indicated that BOS-102 induced cell apoptosis by inhibiting the PI3K/Akt signaling pathway.

BOS-102 Suppresses the PI3K/Akt Signaling Pathway
When apoptosis occurs, the PI3K/Akt signaling pathway plays an important role, which is also an important modulator in ROS-related cell apoptosis. In our study, we used Western blot analysis to detect the expression levels of PI3K/Akt after treatment of BOS-102. As shown in Figure 6A, the phosphorylation of Akt and PI3K were decreased in a dose-dependent manner after BOS-102 treatment. Furthermore, pretreatment of antioxidant NAC, a ROS inhibitor, could efficiently reverse the BOS-102-induced Akt phosphorylation ( Figure 6B). These results indicated that BOS-102 induced cell apoptosis by inhibiting the PI3K/Akt signaling pathway.

BOS-102 Activates the P38/ERK Signaling Pathway
It is well known that the MAPK signaling pathway plays a critical role in regulating cell apoptosis. The effect of BOS-102 on this pathway was detected using Western blot. As shown in Figure 7, at various concentrations after application of BOS-102, the phosphorylation of ERK and p38 were significantly increased. These results indicated that the ERK/p38 signaling pathway was involved in BOS-102-induced apoptosis.

BOS-102 Activates the P38/ERK Signaling Pathway
It is well known that the MAPK signaling pathway plays a critical role in regulating cell apoptosis. The effect of BOS-102 on this pathway was detected using Western blot. As shown in Figure 7, at various concentrations after application of BOS-102, the phosphorylation of ERK and p38 were significantly increased. These results indicated that the ERK/p38 signaling pathway was involved in BOS-102-induced apoptosis.

BOS-102 Activates the P38/ERK Signaling Pathway
It is well known that the MAPK signaling pathway plays a critical role in regulating cell apoptosis. The effect of BOS-102 on this pathway was detected using Western blot. As shown in Figure 7, at various concentrations after application of BOS-102, the phosphorylation of ERK and p38 were significantly increased. These results indicated that the ERK/p38 signaling pathway was involved in BOS-102-induced apoptosis.

Discussion
Non-small cell lung cancer is the most common form of lung cancer globally. Currently, surgery and radiotherapy are available therapeutic approaches for lung cancer patients, but both of them cause severe pain and side-effects [2]. It is highly desirable to develop safe and effective drugs to treat lung cancer and to reduce the pain of patients with lung cancer. In the current study, we reported that BOS-102 could inhibit the proliferation of several cancer cell lines, including human lung cancer cell line A549 (IC 50 : 4.29 ± 0.79 µM), human hepatoma cell line HepG2 (IC 50 : 13.87 ± 1.40 µM), human primary glioblastoma cell line U87 MG (IC 50 : 23.98 ± 8.80 µM) and human pancreatic cell line PANC-1 (IC 50 : 12.48 ± 1.66 µM). In addition, BOS-102 could inhibit the proliferation of normal cells such as human umbilical vein endothelial cells (HUVECs). In the following experiment, we demonstrated that BOS-102 significantly inhibited colony formation in A549 cells ( Figure 2B,C), which was largely due to the effect of BOS-102 induced G0/G1 cell cycle arrest and apoptosis. Furthermore, we investigated the molecular mechanisms of anti-cancer effect of BOS-102, which was likely mediated through various signaling pathways.
As we all know, there are two main methods for cell growth inhibition: cell cycle arrest and apoptosis. In mammalian cells, cell-cycle progression is controlled by a series of cyclin-dependent kinase (CDK)-cyclin complexes. CDK family members, such as CDK2, CDK4, and CDK6, play a key role in the cell-cycle control of tumor cells [20]. Our results showed that the proportion in the G1 phase was significantly increased from 46.06% to 74.37% after treatment of BOS-102 for 48 h ( Figure 4A,B), denoting that BOS-102 induced G0/G1 arrest in A549 cells. Furthermore, the mechanisms of BOS-102-induced cell cycle arrest were investigated using Western blot analysis. Our results showed that the expression of cyclin D1 and CDK4 was decreased after treatment of BOS-102 in A549 cells ( Figure 4C). As we all know, activation of cyclin D1 is important when cells were transiting through G1 into the S phase, then the activated cyclin D1 binds to CDK4 and, finally, induces cells from G1 to the S phase [21,22]. In our study, the expression of cyclin D1 and CDK4 were decreased after treatment of BOS-102, indicating that BOS-102 could exert its anti-proliferative effect via inducing G0/G1 cell cycle arrest, and the molecular mechanisms may be through the modulation of cyclin D1 and CDK4.
Apoptosis is a normal physiological phenomenon that can be observed in various tissues and cells. When apoptosis happens, various morphological changes will appear, such as cell surface changes, nuclear pyknosis, DNA fragmentation, and chromosome condensation [23]. Morphological changes in A549 cells were analyzed by Hoechst 33258 staining ( Figure 3C). The phosphatidylserine externalization (cell surface changes) in A549 cells was detected by flow cytometry after staining with Annexin V-FITC/PI, and our results showed that BOS-102 induced A549 apoptosis in a concentration-dependent manner ( Figure 3A,B). In order to detect whether caspase was involved in BOS-102-induced apoptosis, we used the pan-caspase inhibitor, Z-VAD-FMK, which can inhibit caspase processing and apoptosis induction in tumor cells in vitro. In our study, Z-VAD-FMK inhibited BOS-102-induced A549 apoptosis and cell hypoproliferation ( Figure 3D). These experiments indicated that BOS-102 could induce apoptosis in A549 cells via a caspase-dependent pathway.
It is well known that apoptosis can be activated by various stressors, among which ROS is the major cause of toxicity. It has been reported that ROS acts as a second messenger in the signal process [7,24]. ROS can induce apoptosis via a variety of mechanisms. For instance, increased ROS can active the intrinsic pathway by stimulating the depolarization of MMP [25]. The depolarization of MMP can activate the intrinsic apoptosis pathway and be tightly regulated bythe Bcl-2 family, such as Bcl-2 (anti-apoptotic protein) and Bax (pro-apoptotic protein) [26]. In the present study, an increase of ROS generation was observed in the BOS-102-treated A549 cells ( Figure 5A-C). Furthermore, the ROS generation ability of BOS-102 could be inhibited by NAC, a specific inhibitor of ROS ( Figure 5D). Our results also clearly indicated that MMP was collapsed after treatment of BOS-102 ( Figure 5E,F). As we all know, the loss of MMP leads to cytochrome c release, and the released cytochrome c can activate caspase-3 and PARP. In this study, after BOS-102 treatment, the expression of Bax was increased while Bcl-2 was decreased ( Figure 3E). Additionally, cytochrome c was released to cytoplasm ( Figure 5G), resulting in caspase and PARP activation ( Figure 3F) and cell apoptosis ( Figure 3A,B). In general, our data demonstrated that BOS-102 could induce A549 cells apoptosis via the ROS-mediated mitochondria pathway.
The PI3K/Akt pathway plays an important role in regulating cell survival and death [27]. Therefore, the suppression of the PI3K/Akt signaling pathway may be an effective approach to the treatment of human lung cancer. Phosphorylated Akt can promote the apoptosis-related protein Bcl-2 and cell cycle-related protein cyclin D. Our study found that BOS-102 treatment could induce decreased phosphorylation of PI3K and Akt ( Figure 6A). In our previous study, our results suggested that ROS generation played an important role in BOS-102-induced apoptosis, whether the PI3K/Akt pathway was also connected with ROS generation. In the following study, pretreatment of NAC could efficiently reverse the BOS-102-induced PI3K and Akt phosphorylation ( Figure 6B), suggesting the PI3K/Akt pathway might also be implicated in BOS-102-induced apoptosis, and this process was via excessive generation of ROS.
Mitogen-activated protein kinase (MAPK) signaling pathways also play an important role in the development of cancer. An increase in intracellular ROS can activate MAPK signaling pathways, including p38 MAPK and ERK1/2 [12]. It has been demonstrated that p38 and ERK play a vital role in cell survival and apoptosis [13]. On the one hand, some literatures reported that ERK activation was beneficial to cell proliferation and survival [28]. On the other hand, there is also some literature reported that ERK activation could induce cell apoptosis [4,13,29]. In our study, we found that the phosphorylation of p38 and ERK were increased after treatment of BOS-102 (Figure 7), indicating that the MAPK signaling pathway was also related to BOS-102-induced apoptosis in A549 cells.

Cell Culture
Human lung cancer cell line A549 cells and human pancreatic cell line PANC-1 cells were cultured in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin. Human hepatoma cell line HepG2 cells and human primary glioblastoma cell line U87 MG cells were cultured in Eagle's Minimum Essential Medium (MEM) supplemented with 10% FBS, 100 U/mL penicillin and 100 µg/mL streptomycin. Human umbilical vein endothelial cells (HUVECs) were cultured in Medium 200-supplemented LSGS. Cells were cultured at 37 • C in humidified CO 2 (5%).

Colony Forming Assay
A549 cells were seeded in 6-well plates at a density of 500 cells per well. After 24 h, cell were treated with BOS-102 (0, 2.5, 5, 10 µM). Then cells were incubated for 10 days to allow for colony formation. After staining with crystal violet, colonies containing more than 50 cells were counted and evaluated [30].

Assays for Apoptosis
Cell apoptosis analysis was detected using Annexin V/PI staining assay. Briefly, cells (1 × 10 5 cells/well) were seeded in a six-well plate and incubated for 24 h. Then, cells were treated with BOS-102 (0, 2.5, 5, 10 µM) for 48 h. Cells were harvested, washed with cold PBS, and stained with Annexin V-FITC (Annexin V-Fluorescein isothiocyanate) and PI (propidine iodide). Then cells were analyzed in three different experiments using flow cytometry (Becton Dickinson, Franklin Lakes, NJ, USA).

Flow Cytometric Analysis of Cell Cycle
A549 cells were seeded into 6-well plates at a density of 5 × 10 5 cells per well and treated with BOS-102 (0, 2.5, 5, 10 µM) for 48 h. Cells were harvested, fixed in cold 75% ethanol at −20 • C overnight. Cells were washed with PBS, re-suspended with cold PBS containing 20 µg/mL RNaseA and 50 µg/mL PI. After incubation at room temperature in the dark for 30 min. Cells were analyzed in three different experiments using flow cytometry (Becton Dickinson, Franklin Lakes, NJ, USA).

Morphological Analysis of Apoptosis
Cell morphology of apoptosis cells was evaluated by Hoechst 33258 staining. A549 cells were treated with BOS-102 (0, 2.5, 5, 10 µM) for 48 h. Then cells were washed with PBS and stained with Hoechst 33258. After washing twice with PBS, cells were visualized on a fluorescence microscope (Olympus, Tokyo, Japan).

Analysis of the MMP
MMP was assessed using flow cytometry after staining with JC-1. Briefly, A549 cells (1 × 10 6 ) were seed in six-well plates and treated with BOS-102 (0, 2.5, 5, 10 µM) for 48 h. Cells were harvested and incubated with JC-1 at 37 • C for 20 min. After two washes with PBS, cells were analyzed using flow cytometry (Becton Dickinson, Franklin Lakes, NJ, USA).

Western Blot Analysis
A549 cells were treated with various concentrations of BOS-102 (0, 2.5, 5, 10 µM) for 48 h. Cells were lysed in RIPA lysis buffer on ice for 15 min. The protein concentrations of the cell lysates were determined with a BCA protein kit (Beyotime, Nanjing, China). Proteins were mixed with loading buffer containing 5% 2-mercaptoethanol and then heated for 5 min at 95 • C. Protein lysates were subsequently loaded into each lane of a 12% SDS-PAGE and transferred onto PVDF membranes. Membranes were blocked in 5% (w/v) non-fat milk for 1 h. Then, the membranes were probed with a primary antibody at 4 • C overnight, and incubated with HRP-conjugated secondary antibody. Finally, proteins were visualized using a BeyoECL Plus enhanced chemiluminescence system (Beyotime, Nanjing, China). The protein level was normalized to β-actin. Protein bands were quantified with Image J (Image 2×, NIH, Bethesda, MD, USA).

Statistical Analysis
Statistical analysis was performed using GraphPad Prism 5.0 (San Diego, CA, USA). The data were presented as mean ± SD. Statistical comparisons were performed using one-way analysis of variance. Value of p < 0.05 was considered statistically significant.

Conclusions
In summary, our study extensively evaluated the anti-proliferative effect of BOS-102 in human lung cancer cells, which was mediated through the arresting cell cycle in the G0/G1 phase and inducing cell apoptosis. Furthermore, our results provided evidence that BOS-102 induced apoptosis in A549 cells through ROS-mediated inhibition of PI3K/Akt and activation of p38/ERK signaling pathways ( Figure 8). Overall, our study indicated that the novel bromophenol derivative BOS-102 has the potential to develop into an anticancer drug.

Western Blot Analysis
A549 cells were treated with various concentrations of BOS-102 (0, 2.5, 5, 10 µM) for 48 h. Cells were lysed in RIPA lysis buffer on ice for 15 min. The protein concentrations of the cell lysates were determined with a BCA protein kit (Beyotime, Nanjing, China). Proteins were mixed with loading buffer containing 5% 2-mercaptoethanol and then heated for 5 min at 95 °C. Protein lysates were subsequently loaded into each lane of a 12% SDS-PAGE and transferred onto PVDF membranes. Membranes were blocked in 5% (w/v) non-fat milk for 1 h. Then, the membranes were probed with a primary antibody at 4 °C overnight, and incubated with HRP-conjugated secondary antibody. Finally, proteins were visualized using a BeyoECL Plus enhanced chemiluminescence system (Beyotime, Nanjing, China). The protein level was normalized to β-actin. Protein bands were quantified with Image J (Image 2×, NIH, Bethesda, MD, USA).

Statistical Analysis
Statistical analysis was performed using GraphPad Prism 5.0 (San Diego, CA, USA). The data were presented as mean ± SD. Statistical comparisons were performed using one-way analysis of variance. Value of p < 0.05 was considered statistically significant.

Conclusions
In summary, our study extensively evaluated the anti-proliferative effect of BOS-102 in human lung cancer cells, which was mediated through the arresting cell cycle in the G0/G1 phase and inducing cell apoptosis. Furthermore, our results provided evidence that BOS-102 induced apoptosis in A549 cells through ROS-mediated inhibition of PI3K/Akt and activation of p38/ERK signaling pathways ( Figure 8). Overall, our study indicated that the novel bromophenol derivative BOS-102 has the potential to develop into an anticancer drug.