Monacyclinones, New Angucyclinone Metabolites Isolated from Streptomyces sp. M7_15 Associated with the Puerto Rican Sponge Scopalina ruetzleri

During an investigation of new actinomycete species from Caribbean sponges for novel bioactive natural products, frigocyclinone (1), dimethyldehydrorabelomycin (3) and six new angucyclinone derivatives were isolated from Streptomyces sp. strain M7_15 associated with the sponge Scopalina ruetzleri. Of these, monacyclinones A–B (4–5) contain the core ring structure of dehydrorabelomycin (2) with the aminodeoxysugar found in frigocyclinone (1). Monacyclinone C (6) is a hydroxylated variant of frigocyclinone (1) and monacyclinone D (7) is a Baeyer Villiger derivative of (6) which also exists as the open chain hydrolysis product monacyclinone E (8). Monacyclinone F (9) contains two unique epoxide rings attached to the angucyclinone moiety and an additional aminodeoxysugar attached through an angular oxygen bond. All structures were confirmed through spectral analyses. Activity against rhabdomycosarcoma cancer cells (SJCRH30) after 48 h of treatment was observed with frigocyclinone (1; EC50 = 5.2 µM), monacyclinone C (6; 160 µM), monacyclinone E (8; 270 µM), and monacyclinone F (9; 0.73 µM). The strongest bioactivity against rhabdomycosarcoma cancer cells and gram-positive bacteria was exhibited by compound 9, suggesting that the extra aminodeoxysugar subunit is important for biological activity.

Due to the promising diversity of sponge associated Streptomyces sp. and their ability to produce secondary metabolites, sponges in the Caribbean were screened for the presence of Streptomyces sp. A unique isolate (Streptomyces sp. M7_15) was cultivated from the Puerto Rican sponge Scopalina ruetzleri [15] collected from Mona Island [16]. The main compound produced by Streptomyces sp. M7_15 was found to be the angucyclinone derivative frigocyclinone which was previously isolated from a Streptomyces griseus strain cultured from the soils of Antarctica [17].
Angucyclinone natural products have been isolated from a variety of actinobacteria since the discovery of tetrangomycin and tetrangulol in the 1960s, and congeners of this increasingly diverse natural product class have exhibited intriguing activity profiles [10,11,18]. Frigocyclinone (1) was the first angucyclinone derivative to have a C-glycosidic linked aminodeoxy sugar moiety (Figure 1). In this study we report six related derivatives, called the monacyclinones, which contain similar features to frigocyclinone including the characteristic C-glycosidic aminodeoxy sugar moiety. Significantly, the wild-type strain also produces monacyclinone D (7) in which the A ring is found as a lactone moiety, which most likely arises by a Baeyer-Villiger reaction. In addition, this compound co-occurs with the open chain hydrolysis product monacyclinone E (8). Monacyclinone F (9) contains two epoxide rings attached to the angucyclinone moiety and an additional aminodeoxysugar unit, but this time attached by an ether linkage ( Figure 1). These novel compounds displayed biological activity against human rhabdomyosarcoma cancer cells (SJCRH30) and gram-positive bacteria.

Purification and Structure Elucidation of Angucyclinone Derivatives
Frigocyclinone (1) was previously isolated and characterized from a species of Streptomyces griseus isolated from soils of Antarctica [17]. This compound has a very unusual C-glycosidic linked aminodeoxy sugar moiety attached to an anthraquinone chromophore that displays a characteristic UV absorption spectrum. Upon analysis of the liquid chromatography-diode array detection-electrospray ionization mass spectrometry (LC-DAD-ESIMS) data of fractions from Streptomyces sp. strain M7_15, the 80%-100% MeOH fractions showed a UV absorbance spectrum similar to frigocyclinone (287, 314, and 405 nm). Further purification of this fraction yielded 13 mg of a compound with a molecular weight of 463 Da.
Comparison of all 1 H NMR and 13 C NMR data for 3 were in agreement with those reported by Tibrewal et al. [20] (Table S2).
Monacyclinone A (4, 4.3 mg) was isolated as a yellow powder and was calculated to have a molecular formula of C27H28NO5 ([M + H] + observed: m/z 446.1974, calculated: m/z 446.1967, Δm = 1.6 ppm) as determined by HRESIMS, and corresponding to an additional double bond equivalent compared with 1 (Figure 2), and a possible loss of H2O. The UV spectrum of compound 4 in pyridine exhibited maximum absorbance at 280, 315, and 422 nm. Analysis of the 1 H and 13 C NMR spectral data for 4 (Table 1) showed many similarities with the chemical shift values corresponding to the frigocyclinone sugar moiety, which was attached to the aromatic nucleus at C-9 based on an HMBC correlation between H-1′ and C-9 ( Figure 1 and  Figure 2). The chemical shifts of the A ring aromatic protons (H-2 δH 7.33 s; H-4 δH 7.28 s) were consistent with those of dehydrorabelomycin (2). The assignment of the relative configuration at carbons C-1′, C-4′, C-5′ was based on intense ROESY cross peaks between protons H-1′ (δH 5.26ax), H-3′ at (δH 1.98ax), and H-6′ at (δH 1.52ax) which positions these protons on the same side of the angucyclinone moiety (Table S3; Figure 3). Additionally, ROESY cross peaks between proton H-2′ax (δH 1.44) and H-4′ax at (δH 2.82) indicated diaxial interactions on the opposite face of the sugar ring ( Figure 3).
Monacyclinone B (5, 7.4 mg) was isolated as an orange powder and was calculated to have a molecular formula of C27H28NO6 ([M + H] + observed: m/z 462.1917, calculated: m/z 462.1917, Δm 0.0 ppm) corresponding to an additional oxygen compared with 4, and shared a similar UV absorption spectrum (299, 328, and 445 nm). The difference in molecular formulae suggested 5 contained an additional phenolic group as indicated by the appearance of a new quaternary carbon shift at δ 157.4 in the 13 C NMR spectrum (Table 1). An HMBC correlation from the singlet aromatic proton at (H-6, δH 8.01) to C-4a (δC 124.2) and C-7 (δC 189.9) implied that the phenolic substituent resided on C-5, and further evidenced by the upfield shift of the aromatic proton at C-6 (δC 105.6) in 5 when compared to 4. Comparison of the 1 H and 13 C NMR signals for the rest of the molecule with those observed for 4 supported the proposed structure (Table S4), including the presence of the aminodeoxy sugar with identical configuration to the corresponding moiety in 4.
The molecular formula of monacyclinone C (6, 1.5 mg) (C27H30NO7 [M + H] + , observed: m/z 480.2027, calculated: m/z 480.2022, Δm = 1.0 ppm) indicated 14 double bond equivalents and an additional oxygen atom compared with frigocyclinone (1) (Figure 2). This was consistent with the UV spectrum of 6 which showed absorption maxima at 280, 301 and 429 in accordance with the UV absorption profile of frigocyclinone (1). Much of the 1D and 2D NMR data for 6 was identical to 1: For example, the characteristic C-1 carbonyl shift at δC 196.9, as well as HMBC correlations between the two methylene groups at C-2 (CH2 δH 3.22 m, 2H) and C-4 (CH2 δH 3.25 m, 2H) with the methyl substituent at C-3 (δH 1.56 s, 3H; δC 30.1) (Figure 3), indicated that ring A was unchanged (Table 1, Figure 2). In addition, the usual 1D and 2D NMR data established an aminodeoxysugar group attached to C-9 of the anthraquinone moiety (Table S5). Similar to monacyclinone B (5), the 1 H NMR data of 6 (Table 1) showed the loss of an aryl proton, while the 13 C NMR spectrum now contained a quaternary carbon at δC 164.5 consistent with a phenolic substituent. However in contrast to 5, HMBC correlations in the B ring of 6 were observed from the singlet aromatic proton H-5 (δH 7.16) to C-4 (δC 45.0) and C-6 (δC 164.5), indicating that the phenolic group resided on C-6.     Monacyclinone D (7, 2.2 mg) was isolated as a bright yellow powder and the molecular formula of C27H30NO7 ([M + H] + observed: m/z 480.2022, calculated: m/z 480.2022, Δm = 0.0 ppm) was determined by HRESIMS indicating 14 double bond equivalents. Although 7 was found to have the identical molecular formula of 6, several notable differences were found in the NMR spectra of 7 compared to 6. The 1 H and 13 C NMR spectral data of 7 (Table 1)  Most significantly, the 1 H NMR data revealed that H-5 now appeared as a doublet (δH 7.53, J = 8 Hz) and was coupled with a proton at H-6 (δH 8.05, d, J = 8 Hz), as seen in 1 and 4. Additionally the 13 C spectrum showed that C-12b was considerably deshielded in 7 (Table 1), consistent with an oxygen atom bonded to this carbon. A significant difference was observed in the chemical shift of the C-1 carbonyl in 7 (δC 172.9) compared with 6 (δC 196.9), suggesting the presence of an ester or carboxylic acid functionality. In addition, 7 also contained the two methylene groups both of which appeared as an AB quartet (C-2: CH2 δH 3.21 d, J=17 Hz, 3.23 d, J = 17 Hz and C-4: CH2 δH 3.21 d, J = 17 Hz, 3.81 d, J = 17 Hz). Both also displayed HMBC correlations with the methyl substituent at C-3 as observed with 6 (Table S6; Figure 1). These results, in addition to the double bond equivalents calculated from the molecular formula, indicated that 7 contains a seven membered lactone ring formed by a Baeyer Villiger oxygenation reaction [21]. A similar angucyclinone containing a lactone moiety was identified in trace amounts from a mutant strain of S. fradiae [22], but our work represents the first report of such a compound obtained from a wild-type strain.
Many of the NMR spectral features of monacyclinone E (8, 1.8 mg) (C27H32NO8, [M + H] + observed: m/z 498.2124, calculated: m/z 498.2128, Δm = −0.8 ppm) were similar to those of frigocyclinone (1) and monacyclinone D (7). The 1 H and 13 C NMR 2D spectral data of 8 (Tables 1 and S7) indicated no changes to the aminodeoxysugar substituent, including the point of attachment at C-9 as indicated by an HMBC correlation between the aromatic carbon C-9 (δC 141.0) and the anomeric proton H-1′ (δH 5.22 m). The other spectral data (Table 1) 1, 4, and 7. The 13 C resonance for C-1 was observed at a higher field in 8 (δC 175.7) compared with 6 (δC 196.9) (Table 1) and the chemical shift for C-12b appeared at δC 162.6, both of which suggested similarity to 7. Based on these chemical shift changes, the loss of one double bond equivalent indicated by the molecular formula revealed that 8 was the result of an oxidative opening of the lactone ring in 7. A similar ring opening has been observed in fridamycin D [8].
Metabolites 4-8 shared similar structural features with the previously reported frigocyclinone and dehydrorabelomycin. In all compounds the aminodeoxysugar was present and attached through a C-glycosidic bond to the C-9 of the angucyclinone chromophore. However, an additional metabolite (compound 9) was isolated from the fermentation extract and found to have a second aminodeoxy sugar attached through an angular oxygen to the angucyclinone chromophore.
Monacyclinone F (9, 8.3 mg) was isolated as a purple oil and found to have the molecular formula C35H47N2O10 ([M + H] + observed: m/z 655.3253, calculated: m/z 655.3231, Δm = 3.4 ppm) by HRESIMS indicating 14 double bond equivalents ( Figure 2). The UV absorption spectrum of 9 showed absorption maxima at 281, 298, and 367 nm. The 1 H and 13 C NMR spectral data of 9 (Table 2) showed the presence of only two ortho coupled aromatic protons H-10 (δH 8.14 d, J = 8 Hz) and H-11 (δH 8.03 d, J = 8 Hz), consistent with a C/D ring system as found in 1 and 4-8 ( Figure 2, Table 2). Once again, the chemical shift data established an aminodeoxy sugar attached in the usual fashion to C-9 of ring D as well as a phenolic group at C-8 (δC 159.2). In contrast, the protons at C-5 and C-6 now appeared as a methylene group δH 2.83 (m) and a methine δH 4.69 (m), respectively. Furthermore, the upfield chemical shift of the carbons in ring B (C-4a (δC 82.8), C-5 (δC 36.7), C-6 (δC 64.5), C-6a (δC 58.5), C-12a (δC 71.3), and C-12b (δC 80.5), established a loss of aromaticity in this ring. Thus the number of double bond equivalents must be accounted for as rings, and based on analysis of HMBC and HSQC NMR data ( Table 2, Figures S18 and S19) it was found that two of the additional oxygen atoms in the molecular formula of 9 could be accounted for as epoxide rings. One of the epoxide rings included carbons C-6 (δC 64.5) and C-6a (δC 58.5), while the second contained carbons C-12a (δC 71.3) and C-12b (δC 80.5) (Table 2, Figure 1, Table S8). Further HMBC analysis revealed a strong correlation between C-4a (δC 82.8) and the anomeric proton H-1′′ (δH 5.75 s), identifying the presence of a second aminodeoxysugar (Table S8), thus accounting for the final double bond equivalent. The chemical shift of the anomeric carbon C-1′′ (δC 94.1) indicated that this sugar moiety was attached to the central core through a more common ether link and not through a C-glycosydic bond. There were no other significant changes to ring A as suggested by the presence of the two methylene groups at C-2 (CH2 δH 2.83 dd J = 18, 3 Hz, δH 3.34 dd J = 18, 3 Hz) and C-4 (CH2 δH 2.12 m, 2.93 dd J = 14, 3 Hz) that showed HMBC correlations with the methyl substituent at C-3 ( Table 2; Table S8; Figure 1). The relative configuration of both aminodeoxy sugar moieties were found to be identical to that observed in frigocyclinone based on ROESY correlations between the axial hydrogen atoms as described above (Figures 1 and 3).

Cultivation of a Possible New Phylotype of Streptomyces sp.
The closest relative to Streptomyces sp. M7_15 searched through GenBank was Streptomyces sp. CNS-669-SD06 with a supporting 98% sequence homology of a partial sequence of the 16S rRNA gene (Figure 4). This suggests the possibility that Streptomyces sp. M7_15 is a new phylotype [23] with the ability to produce new compounds, and in fact six new compounds related to frigocylinone were isolated from the EtOAc extract of the fermentation broth ( Figure 2). Since frigocyclinone was previously isolated from a Streptomyces griseus species associated with soils of Antarctica, its partial 16S rRNA sequence was used to compare its genomic information to the 16S rRNA gene of Streptomyces sp. M7_15. Intriguingly, when the neighbor-joining tree was analyzed, these two strains were grouped into two separate clusters, showing a significantly distant relationship to one another ( Figure 4). Therefore, it could be assumed that Streptomyces sp. M7_15 is a new actinomycete phylotype that has evolved similar biosynthetic genes to the Antarctic strain of S. griseus or that the presence of the pathways leading to angucyclinone production in one or both strains resulted from horizontal transfer and the two pathways have a common ancestor [24][25][26]. Distinguishing these two possibilities would require sequencing both pathways.

Biosynthetic Diversity of Angucyclinone Derivatives
Aromatic polyketides biosynthesized by a type II PKS are divided into anthracyclines, angucyclines, and tetracyclines [10,11]. Derivatives of these can undergo further oxygenation, glycosylation and dehydration and show a wide range of biological activities [12,13,21,27]. The monacyclinones isolated in this study provide examples of many such post-PKS modifications of the angucyclinone moiety, and in some cases these modifications are extremely rare. It is possible to speculate that the early biosynthetic products of this organism are frigocyclinone (1) or monacyclinone C (6) which undergo a series of further modifications. For example, either of these compounds could serve as precursors of 7 (and its hydrolysis product (8) following a Baeyer-Villiger oxygenation reaction. Such a reaction has been reported once in the generation of the angucyclinone derivative urdamycin L, isolated from a mutated strain of the soil bacterium Streptomyces fradiae [22]. Urdamycin L, which possesses a seven-membered lactone moiety in ring A [21,22], is very similar in structure to monacyclinone D (7) and only lacks the C-linked deoxyamino sugar at C-9. It is proposed that lactone production is catalyzed by the protein oxygenase UrdM [22], and might be the pathway to hydroxylation at C-12b. Certainly the discovery of the monacyclinones 7-8 in a wild-type strain of a Streptomyces sp., tends to support this proposal. It is also likely that two separate glycosyltransferase enzymes operate in the C-and O-glycosides found in monacyclinone F (9) as observed in other angucyclinone derivatives [11]. Another rare modification is the formation of two epoxide rings at positions 12a and 6 in monacyclinone F (9) and an angular oxygen at position C-4a. The placement of angular oxygens at C-12b and C-4a are widespread throughout many angucycline derivatives where the oxygens at these positions arise from acetate (C-4a) and atmospheric oxygen (C-12b) via introduction by a monooxygenase [10]. The monooxygenases responsible for these modifications are characterized as flavin-dependent monooxygenases and a recent study on these tailoring enzymes found enormous functional flexibility and the ability to catalyze alternate reactions depending on the substrate, reaction conditions, and presence of other enzymes [28,29]. Flavin-dependent monooxygenases catalyze a variety of reactions, including hydroxylation, Baeyer-Villiger oxidation, and epoxidation. The structural diversity of the monacyclinones underscores the catalytic flexibility of these tailoring enzymes resulting in the immense structural diversity of the angucycline class of natural products.

Biological Activity of Angucyclinone Derivatives
Frigocyclinone (1), exhibited weak antibiotic activity against Bacillus subtilis [17]. In addition, frigocyclinone showed an inhibition zone of 1 mm at a concentration of 1 mg/mL against Mycobacterium smegmatis (Table S9). No inhibition was observed against gram negative or fungal organisms at the concentrations tested. This compound also showed cytotoxicity against human rhabdomyosarcoma SJCRH30 cells with an EC50 value of 5.2 µM at 48 h post treatment (Table 3; Figures S20 and S21). Modifications to the angucyclinone moiety resulted in a wide variety of bioactivity profiles against the human rhabdomyosarcoma SJCRH30 cells for the monacyclinone derivatives (Figures S20 and S21; Table 3). Of all the derivatives tested, compound 9, which has two aminodeoxysugars attached to the angucyclinone moiety, exhibited the strongest cytotoxicity against bacteria and human rhabdomyosarcoma SJCRH30 cells. This metabolite registered EC50 values of 610 nM and 730 nM 24 h and 48 h post treatment, respectively (Table 3). In contrast to compound 9, compounds 4-8 with an aromatic ring and only one aminodeoxysugar unit showed weak to no bioactivity against gram-positive bacteria and human rhabdomyosarcoma SJCRH30 cells (Table 3; Table S9). Moreover compound 3, that lacks both aminodeoxysugars, shows weak bioactivity in all the bioassays conducted (Tables 3 and S9).
As indicated by their structures, these bioactivity results suggest that the aminodeoxysugar subunit, the epoxide groups, and the ketone moiety could all be important for biological activity.

General Experimental
All media components and chemicals were purchased from Fisher Scientific (Waltham, MA, USA). Optical rotation data were recorded on a Rudolph Research Analytical Autopol III automatic polarimeter (Rudolf Research, Fairfield, NJ, USA). UV spectra were recorded on a Beckman DU 640 spectrophotometer (Beckman Instruments, Fullerton, CA, USA). 1 H NMR, COSY, HSQC, and HMBC spectra were recorded in methanol-d4 or pyridine-d5 using a 500 MHz Bruker NMR Avance spectrometer (Bruker, Billerica, MA, USA). Chromatography for LC/MS was performed using a Hewlett-Packard

Isolation of Streptomyces sp. M7_15
Strain Streptomyces sp. M7_15 was isolated from the Caribbean marine sponge Scopalina ruetzleri. The sponge S. ruetzleri was collected by SCUBA at a depth of 20 m on Las Carmelitas dive site located in Mona Island, Puerto Rico (18°6′25.72″ N, 67°56′11.35″ W). A volume of 1 mL of sponge tissue was scraped from the coral bottom with a sterile razor blade and transferred to a 50-mL sterile tube with sterilized tweezers. Sterile mortars and pestles were used to homogenize the sponge before dilution. The sponge homogenate was then treated with heat (55 °C for 60 min) and agitation (vortexed for 1 min) in order to select for actinomycetes. Media M6 (1 L 75% ocean seawater, 500 mg mannitol, 100 mg peptone and 18 g agar [30]) was used to isolate Streptomyces sp. M7_15. M6 media was supplemented with 50 μg/mL of the fungicide cyclohexamide and 20 μg/mL of the antibiotic novobiocin to facilitate the isolation of actinobacteria and deter the growth of sponge associated fungi and gram-negative bacteria on the plates [30,31]. Aliquots of 100 μL were transferred from the S. ruetzleri homogenate dilution to the M6 media. After 8 weeks of incubation of the sponge homogenate from Scopalina ruetzleri, the actinomycete strain Streptomyces sp. M7_15 appeared as a brown circular colony that produced white spores and a dark purple diffusible pigment. The strain is kept in culture on agar slants as well as cryopreserved.

DNA Extraction, Sequencing and Phylogenetic Analysis
DNA was extracted from a 20 mg pellet of cells from a pure isolate using the Gentra Puregene kit (Qiagen, Valencia, CA, USA). The 16S rRNA gene was amplified using polymerase chain reaction (PCR) with universal eubacterial primers 27F and 1492R [32,33]. The PCR mix consisted of 39.3 μL of nuclease free water, 0.2 μL of Platinum Taq DNA Polymerase High Fidelity (Invitrogen, Carlsbad, CA, USA), 1 μL (40 ng/μL) of each primer, and 1 μL (100 ng/μL) of DNA template. The PCR program was set to an initial denaturation at 95 °C for 2.5 min, followed by 35 cycles of 95 °C for 30 s, 45 °C for 30 s, 72 °C for 1 min, and a final extension during the last cycle of 72 °C for 10 min. Results of PCR reactions were observed on 1% agarose gels. Sequencing reactions were conducted with 685R primer and Big Dye terminator (Applied Biosystems, Foster City, CA, USA). The reaction products were analyzed with a 3130XL Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). Sequences were assembled and edited using Sequencher 5.2.4 (Gene Codes, Ann Arbor, MI, USA). Double coverage reads allowed for accurate editing of sequences. The complete consensus sequence was exported into a single Fasta file from Sequencher and uploaded with MEGA 5 (Gene Codes, Ann Arbor, MI, USA) [34]. Sequences were then aligned using the ClustalW function with default parameters (ClustalW, University College Dublin, Dublin, Ireland). The closest relative to our sequence was searched using the BLAST function from GenBank. These sequences were used as reference sequences for alignment and tree generating purposes. MEGA 5 was used to construct a phylogenetic tree of the 16S rRNA gene using a neighbor-joining (NJ) analysis. The NJ analysis was performed using a maximum composite likelihood model of nucleotide substitution and data was re-sampled using 1000 bootstrap replicates.

Chemical Analysis of Actinomycetes
Streptomyces sp. M7_15 was fermented in large scale in P3C media (10 g starch, 4 g yeast, 2 g peptone, 1 g CaCO3, 40 mg Fe2SO4, 100 mg KBr, in 1 L of 75% seawater) over a period of 3 months. Batches (1.6 L total volume) of Streptomyces sp. M7_15 were cultured at 28 °C on a rotary shaker (IKA, Wilmington, NC, USA) in 500 mL flasks containing 100 mL of media each during a period of 9 days on a rotary shaker (200 rpm). Cells and fermented broth were harvested by vacuum filtration using glass fiber filters (GF/C) (Whatman, Maidstone, UK). Media was extracted by liquid-liquid partitioning using ethyl acetate (EtOAc) and butanol (BuOH) (2:1). The cells retained on the glass fiber filter were extracted with 80% aq.MeOH. Extracts were monitored by LC/MS and tested for antimicrobial activities against gram positive (Bacillus subtilis and Mycobacterium smegmatis), gram negative (Escherechia coli) and fungal (Aspergillus niger and Candida kefyr) organisms.

Purification of Monacyclinones from Streptomyces sp. M7_15
LC/MS analysis and antibiotic assays of the crude EtOAc extract (986 mg) showed potent bioactivity and displayed characteristic UV spectral data (190 nm and 234 nm). Subsequently, this extract was

Image Based Cytotoxicity Assay Using SJCRH30 (Rhabdomyosarcoma) Cells
Cytotoxicity was assessed using a live cell imaged based assay that utilizes the fluorescent nuclear stain Hoechst 33342 (H-dye) (Sigma, St Louis, MO, USA) and the fluorescent microtubule stain Tubulin Tracker 488 (Life Technologies, Gaithersburg, MD, USA) [35]. For this assay, the most sensitive cell line for marine toxins was found to be the SJCRH30 cells. In addition to being very sensitive to marine toxins, these cells are large and spread out which allows for counting efficiency for live cell imaging and allowed for easier measurement and visualization of morphological changes associated with toxin. Comparison of the half maximal effective concentration (EC50) values for the traditional XTT cytotoxicity assay and the in vitro microscopy assay using the human cell line model (SJCHR30) showed no significant difference between the assays [35], therefore the in vitro cytotoxicity assay was used to determine the EC50 values for the marine derived products in this manuscript.
Cytotoxicity was measured using fluorescence staining of cell nucleii. Initially, SJCRH30 cells were seeded at a density of 5000 cells/well in BD biocoat poly-D-lysine-coated 96-well plates (Becton Dickinson, Franklin Lakes, NJ, USA) and incubated at 37 °C overnight. Cells were then treated with 10× solutions of compounds added directly to the growth medium (RPMI 1640 supplemented with 10% FBS) and incubated at 37 °C for the designated time period. Cell nucleii were stained with a 0.1 µg/mL final concentration of Hoechst 33342 (Invitrogen, Grand Island, NY, USA) at the same time as the compound treatment. 10 µL of 10× Hoechst dye in Phosphate Buffered Saline was added directly to the growth medium in the wells, and the plate was incubated at 37 °C for the remaining time. Cells had to be stained in two separate steps because Hoechst 33,342 is not compatible with the Hanks Buffered Saline Solution (HBSS) that is required for the tubulin staining protocol.
To stain microtubules with Tubulin Tracker 488 (TT488), a 500 µM intermediate stock was first prepared by combining 1 mM TT488 (Invitrogen, Camarillo, CA, USA) with an equal volume of 20% Pluronic F-127 in DMSO (Invitrogen, Camarillo, CA, USA). Then a 200 nM TT488 staining solution was prepared from the intermediate stock in HBSS. The growth medium containing the Hoechst dye was removed from the wells, and the cells were rinsed once with warm HBSS (100 µL per well). The HBSS rinse was replaced with warmed 200 nM TT488 staining solution (100 µL per well). The plate was then returned to the 37 °C incubator for 20 min before imaging on an Image Xpress Micro system (Molecular Devices, Sunnyvale, CA, USA), equipped with an environmental control chamber warmed to 37 °C. The intensity of the fluorescence of the Hoechst dye stain was measured on the DAPI channel and that of the TT488 was measured on the FITC channel of the Image Xpress Micro (Molecular Devices, Sunnyvale, CA, USA) using the MetaXpress Image Acquisition and Analysis v2.0.1.44 software (Molecular Devices, Sunnyvale, CA, USA). The percentage of DAPI positively-stained cells as compared to control treatments (percentage of maximum cell survival) averaged within 4 snapshots of each well were calculated with SAS v9.1.3 software (SAS Institute Inc., Cary, NC, USA) and analyzed with non-linear regression curve-fit analysis by GraphPad Prism v4.03 (Graphpad, San Diego, CA, USA) to yield EC50 values. In addition, pictures of cells from the cytotoxicity experiments stained with Hoechst dye and Tubulin Tracker were visualized using the MetaXpress Image Acquisition and Analysis v2.0.1.44 software (Molecular Devices, Sunnyvale, CA, USA). EC50 values were determined using an ANOVA SAS v9.1.3 software (SAS Institute Inc., Cary, NC, USA). In all experiments, results are presented as the mean ± SD and were considered statistically significant if a p-value was less than 0.05.

Conclusions
This study describes six new angucyclinone derivatives which, in addition to frigocyclinone, add to the list of angucyclinones with a C-linked aminodeoxysugar. Several novel features include the second appearance of a lactone ring as part of the angucyclinone moiety (compound 7) and the addition of a second aminodeoxysugar with two epoxide rings (compound 9). These compounds are an example of the remarkable biosynthetic ability of marine sponge-associated Streptomyces sp. to produce bioactive secondary metabolites. Despite the weak biological activities of monacyclinones A-E (4-8), monacyclinone F (9) with an additional aminodeoxysugar moiety showed potent bioactivity against rhabdomyosarcoma SJCRH30 cells. These bioactivity profiles should encourage further bioassays of angucyclinone derivatives against other cancer cell lines.