Diphlorethohydroxycarmalol Inhibits Interleukin-6 Production by Regulating NF-κB, STAT5 and SOCS1 in Lipopolysaccharide-Stimulated RAW264.7 Cells

Diphlorethohydroxycarmalol (DPHC) is a phlorotannin compound isolated from Ishige okamuarae, a brown alga. This study was conducted to investigate the anti-inflammatory effect and action mechanism of DPHC in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. We found that DPHC strongly reduces the production of interleukin 6 (IL-6), but not that of tumor necrosis factor-alpha (TNF-α) induced by LPS. DPHC (12.5 and 100 μM) suppressed the phosphorylation and the nuclear translocation of NF-kappaB (NF-κB), a central signaling molecule in the inflammation process induced by LPS. The suppressor of cytokine signaling 1 (SOCS1) is a negative feedback regulator of Janus kinase (Jak)-signal transducer and activator of transcription (STAT) signaling. In this study, DPHC inhibited STAT5 expression and upregulated that of SOCS1 at a concentration of 100 μM. Furthermore, N-tosyl-l-phenylalanine chloromethyl ketone (TPCK) (a specific NF-κB inhibitor) and JI (a specific Jak2 inhibitor) reduced the production of IL-6, but not that of tumor necrosis factor-alpha (TNF-α) in LPS-stimulated RAW 264.7 macrophages. These findings demonstrate that DPHC inhibits IL-6 production via the downregulation of NF-κB and Jak2-STAT5 pathway and upregulation of SOCS1.

Various marine bio-resources have recently been explored because of the investigations of active components in pharmaceutical and functional food areas [22,23]. Diphlorethohydroxycarmalol (DPHC) was discovered during the determination of anti-inflammatory materials from marine plants living in the Jeju coastal area. DPHC has been isolated from a brown alga called Ishige okamuarae. Few studies have been conducted on the useful biological activities of DPHC. This compound exhibited an anti-diabetic effect that alleviated increased postprandial blood glucose levels in diabetic mice and induced factors of high glucose-induced oxidative stress in human umbilical vein endothelial cells [24,25]. However, little information is available regarding the anti-inflammatory activity of DPHC.
In the present study, we investigated the activity and action mechanism of DPHC on the production of IL-6, a pivotal cytokine of the inflammatory process in LPS-stimulated RAW264.7 cells.

DPHC Selectively Inhibits LPS-Induced IL-6 Production in RAW264.7 Cells
IL-6 and TNF-α are representative pro-inflammatory cytokines generated by external stimuli in macrophages. To assess DPHC for its anti-inflammatory effect, we investigated the inhibitory activity of DPHC on LPS-induced IL-6 and TNF-α production, important pro-inflammatory cytokines, in RAW264.7 cells. In order to confirm the effect of DPHC, we simultaneously determined cell viability at various concentrations of DPHC by the water soluble tet razolium salts (WST) assay. As shown in Figure 1B, DPHC is no cytotoxic at the tested concentrations ( Figure 1B). In order to find the time profile of IL-6 and TNF-α production, we measured the amount of IL-6 and TNF-α in culture supernatants at the indicated time points after LPS treatment. After LPS treatment, TNF-α production was induced after 1 h and continuously increased up to 4 h and then plateaued ( Figure 1D), whereas the IL-6 production continuously increased from 6 h (312.4 pg/mL ± 10.2) up to 24 h (918.3 pg/mL) ( Figure 1C), and TNF-α was produced earlier than IL-6. These results of the production of IL-6 and TNF-α according to the LPS treatment times are correlated with the kinetics of mRNA expression of inflammatory mediators reported in our previous article [26]. Next, we examined the effect of DPHC on the production of IL-6 and TNF-α induced by LPS. DPHC inhibited the production of IL-6 at 6 h ( Figure 1E) and 24 h ( Figure 1F) in a dose-dependent manner. Especially, the powerful effect of DPHC after 24 h of incubation is shown in Figure 1F. However, DPHC had no effect on the production of TNF-α at both 6 h ( Figure 1G) and 24 h ( Figure 1H) of incubation. These results provide a possibility that there will be a different mechanism in the production of IL-6 and TNF-α in LPS-stimulated macrophages. Most of the bio-active compounds used in our previous studies inhibited the production of IL-6 and TNF-α in LPS-stimulated macrophages [27]. Therefore, we tried to find the point that DPHC selectively inhibits IL-6 production and that will be a different mechanism in the production of IL-6 and TNF-α in LPS-stimulated murine macrophages.  Figure 1F,H). The amounts of IL-6 or TNF-α were measured from the culture supernatants by ELISA. Data are the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. DPHC-untreated cells in the presence of LPS.

DPHC Inhibits the Activation and Nuclear Translocation of NF-κB in LPS-Stimulated RAW264.7 Cells
LPS presentation on monocytes and macrophages may mediate the activation of the NF-κB pathway, leading to the generation of pro-inflammatory cytokines, including IL-6, iNOS, TNF-α. There are many anti-inflammatory compounds isolated from plants inhibiting the production of inflammatory mediators by regulating NF-κB. Lycopene, a red carotenoid pigment occurring in tomatoes and several other ripe fruits, inhibited the LPS-induced production of NO and IL-6 by suppressing the activation of ERK, p38 and NF-κB in LPS-stimulated RAW264.7 cells [28]. Since phosphorylation of NF-κB-p65 is a crucial step in the function of NF-κB-p65, we determined the phosphorylation of nuclear NF-κB-p65 with and without DPHC treatment by Western blot analysis. DPHC inhibited LPS-induced phosphorylation of NF-κB-p65 at a concentration of 12.5 and 100 μM, and this inhibitory effect has been shown at 5, 60 and 360 min after DPHC treatment (Figure 2A,B). To determine whether this inhibition is accompanied by the degradation of IκB-α, we determined the cytoplasmic levels of IκB-α. As shown in Figure 2A,B, the band intensity of IκB-α decreased at 5 min and returned to basal levels at 60 min in the presence of DPHC (100 μM) after LPS stimulation ( Figure 2A). Next, we examined the nuclear translocation of NF-κB (phospho-p65, p50) using confocal laser scanning microscopy. DPHC (100 μM) strongly inhibited LPS-induced nuclear translocation of NF-κB (p65, p50) at 60 and 360 min ( Figure 2C,D). These results suggest that the inhibitory effect of DPHC on the production of IL-6 occurs through inhibiting the activation and nuclear translocation of NF-κB.

DPHC Does Not Affect the MAPK Pathway in LPS-Stimulated RAW264.7 Cells
Stimulation of TLR4 by LPS triggers the activation of the MAPK pathway and results in the production of pro-inflammatory cytokines. There are many anti-inflammatory compounds isolated from plants inhibiting the production of inflammatory mediators by regulating the NF-κB, MAPKs and/or Jak-Stat pathways. Theaflavin, a major polyphenol in black tea, suppressed LPS-induced IL-6, MCP-1 and ICAM-1 expression via blockade of the NF-κB and MAPK pathways in bone marrow-derived macrophages [29]. Thus, we examined the effect of DPHC on LPS-induced MAPK activation by Western blotting at various times after LPS treatment. As the results, DPHC (12.5 and 100 μM) did not inhibit the phosphorylation of three MAPKs (p38, JNK and ERK) induced by LPS treatment ( Figure 3A). On the contrary, DPHC weakly increased the phosphorylation of p-38 and JNK at the times of 15 and 30 min. Therefore, DPHC did not suppress the phosphorylation of the three MAPKs induced by LPS treatment. In the present study, the anti-inflammatory effect of DPHC was associated with the NF-κB pathway, rather than the MAPK pathway.

DPHC Inhibits STAT5 and SOCS1 in LPS-Stimulated RAW264.7 Cells
STATs are a family of nuclear proteins mediating the action of a number of cytokines, such as ILs, IFNs, and others. Especially, the Jak2-STAT5 pathway is known to be involved in LPS-induced IL-6 production. SOCS1 is a negative feedback regulator of JaK-STAT signaling. A recent study reports that SOCS1, an intracellular negative-feedback molecule, selectively inhibits LPS-induced IL-6 production, but not that of TNF-α [21]. They found that LPS directly activates Jak2 and Stat5, whereas SOCS1 inhibits LPS-induced Jak2 and Stat 5. These authors also demonstrated that Stat5 associates with p50 and mediates LPS-induced IL-6 production. It has been demonstrated that SOCS1 selectively inhibits IL-6 production via the regulation of the Jak2-STAT5 pathway in LPS-stimulated macrophages [21]. Therefore, it is possible that STAT5 and SOCS1 participate in the inhibition of IL-6 production by DPHC treatment. Our study supports the present experimental result that DPHC strongly inhibited IL-6 production, but not that of TNF-α. Therefore, we investigated whether Jak-STAT pathway and SOCS1 levels correlated with the inhibition of IL-6 production by DPHC treatment. We first examined whether DPHC affects STAT5 activation in LPS-stimulated Raw264.7 cells. The phosphorylation of STAT5 peaked after 120 min of LPS stimulation, and this activation was reduced by treatment of 100 μM of DPHC. In addition, DPHC (100 μM) reduced the total protein levels of Stat5 ( Figure 3B). We next measured the SOCS1 (an intracellular negative-feedback molecule) level under the same experiment condition of the STAT5 examination. It was found that the SOCS1 level was increased at 240 and 360 min after treatment of 100 μM of DPHC ( Figure 3C). Interestingly, it has not yet been reported that a natural anti-inflammatory compound inhibits only the production of IL-6, not that of TNF-a. Furthermore, the regulation of inflammatory cytokines in response to SOCS1 signaling in LPS-stimulated macrophages has not been well studied.
These results suggest that DPHC inhibits IL-6 production by downregulating STAT5 activation and SOCS1 augmentation. However, it is not clear that the evoked SOCS1 protein by DPHC directly inhibits the Jak2-STAT5 pathway.

N-Tosyl-L-phenylalanine Chloromethyl Ketone and JI Selectively Reduce the Production of IL-6
The above results show that the NF-κB and Jak2-STAT5 pathways are important in the function of DPHC. To determine whether the NF-κB and Jak2-STAT5 pathways limited to IL-6 production, we examined the cytokine level (IL-6 and TNF-α) after the treatment of two known signaling inhibitors in LPS-stimulated RAW264.7 cells. TPCK (N-tosyl-L-phenylalanine chloromethyl ketone, a specific NF-κB signaling inhibitor) and JI (a specific Jak2 inhibitor) strongly reduced the production of IL-6, but not that of TNF-α induced by LPS (Figure 4).

DPHC Suppresses the Development of Experimental Atopic Dermatitis
To induce experimental atopic dermatitis (AD), mice were sensitized by applying 1% 2,4-dinitrochlorobenzene (DNCB) to the abdomen. They were then re-sensitized by applying 0.3% DNCB to the ears on every other day for up to 30 days. Starting on Day 12, the mice were painted with hydrocort cream and DPHC (10 and 100 mg/kg) on the ears on every other day ( Figure 5A). IgE is an important therapeutic target for AD, as it is the major activator of mast cells, which release histamine [30]. Therefore, we measured the levels of serum IgE in mice with dermatitis. The DPHC-treated group showed significantly reduced levels of IgE (p < 0.05) compared with the induction-only group (mice exposed to DNCB, but not having DPHC applied; Figure 5B). The skin lesions associated with AD are characterized by an inflammatory cell infiltrate [31]. Therefore, we next tested whether DPHC reduced the level of inflammatory cell infiltration in the ears of mice with experimental AD. We also examined cutaneous edema as a measure of AD progression. We found that the ear thickness in DPHC-treated mice was reduced at Days 24 and 29 (both p < 0.05) compared with that in induction-only mice ( Figure 5C,D). We next examined the effect of DPHC on the infiltration of inflammatory cells by hematoxylin and eosin (H&E) staining of ear tissue sections. Epidermal thickness and the degree of inflammatory cell infiltration were significantly lower in the DPHC-treated group than in the induction-only group ( Figure 5E). The lymph nodes (LNs) play an important role in cell-mediated immunity by regulating the activity of mature T-and B-cells [32]. Therefore, we examined the morphologic changes in the LN of AD mice. The LNs from mice in the induction-only group were very swollen, whereas those from DPHC mice were smaller and weighed less ( Figure 5F).

Experimental Animals
Female BALB/c mice (7 weeks old) were purchased from Orient Bio (Orient Bio Inc., Seongnam, Korea) and maintained under pathogen-free conditions in the animal facility of Jeju National University. All animal experiments were approved by the Jeju National University Animal Care and Use Committee (2013-0025).

DPHC Isolation
Thalli of Ishige okamuarae were collected on Jeju Island, Korea. A voucher specimen has been deposited at the herbarium of Jeju Biodiversity Research Institute (JBR-85). A shade-dried whole plant of Ishige okamuarae (800 g) was extracted with 70% aqueous ethanol under stirring (750 rpm) for 2 days at room temperature. The filtrate was concentrated under reduced pressure and freeze-dried to obtain a powder. The filtrate was suspended in distilled water and partitioned with ethyl acetate. The ethyl acetate fraction was chromatographed in a reversed phase silica gel using gradient solvent (H2O/MeOH) system to provide four fractions (1-4). Fraction 1 was chromatographed in a Sephadex-LH 20 column (Pharmacia, Stockholm, Sweden) using CHCl3/MeOH (1/1) to provide six fractions (4-1′-4-6′). The Faction 4-5′ contained the DPHC. The structure of DPHC ( Figure 1A) was confirmed by comparing its NMR spectral data with those obtained from previous studies [33,34].

Cell Culture
RAW 264.7 cells, the murine macrophage cell line, were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). Cells were cultured in DMEM supplement with 10% (vol/vol) FBS and 100 U/mL penicillin-streptomycin (GIBCO, Grand Island, NY, USA). The cultured cells were maintained at subconfluence under 5% CO2 humidified atmosphere at 37 °C .

Cell Viability
Cell viability was determined by EZ-CyTox (WST-1) assays (Daeil Lab Inc., Seoul, Korea). RAW264.7 cells were seeded on 96-well culture plates at a density of 1.5 × 10 5 cells/mL and were maintained in an incubator for 18 h. The medium was then replaced with a new medium, DMEM supplemented with 10% FBS and with 100 U/mL penicillin-streptomycin. The cells were treated with LPS in the absence or presence of various DPHC concentrations. After incubation for 24 h, 100 μL of cells were removed from cells from each well. The remaining cells were treated with 5 μL of WST per well in a 5% CO2 atmosphere incubator for 2 h at 37 °C . The light absorbance of each well was quantified using a VersaMax ELISA microplate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm.

ELISA
The 100 μL of cell culture supernatants, mouse serum or standards in a reagent diluent were added to each well of the ELISA kit well plate and incubated for 2 h. The plates were then washed, and 100 μL of the detection antibody in a reagent diluent were added to each well for 2 h. The plates were then washed again, and 100 μL of streptavidin conjugated with horseradish-peroxidase (HRP) was added for 20 min. The plates were then washed, and 100 μL of substrate solution was added to each well in a dark place for 20 min. Then, 50 μL of stop solution were added to each well. The reaction was terminated, and the optical density of each well was determined using a VersaMax ELISA microplate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm.

Western Blot Analysis
Protein quantification of the supernatants was measured by the Bradford assay (Bio-Rad, Hercules, CA, USA). Aliquots of the lysates were separated on a NuPAGE 4%-12% bis-Tris gel (Invitrogen, Carlsbad, CA, USA). The proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane using an iBlot gel transfer device (Invitrogen, Carlsbad, CA, USA). Then, membranes were blocked with 5% non-fat skim milk solution, and the membranes were incubated with primary antibodies at 4 °C overnight. The membranes were then washed several times with Tween 20-Tris-buffered saline (TBST) and incubated with secondary HRP-linked anti-rabbit or anti-mouse IgG, respectively, for 90 min at room temperature. After washing, immunoactive proteins were determined using the WEST-ZOL (plus) Western blot detection system (iNtRON Biotechnology, Gyeonggi, Korea).

Confocal Laser Scanning Microscopy Analysis
Cells were seeded onto coverslips in a 6-well plate and then fixed with 3.5% formaldehyde (PFA) in PBS for 30 min. The cells were washed with PBS, then treated with 0.1 M glycine for 15 min and permeabilized with PBS containing 0.1% Triton X-100 for 10 min. After several washings with PBS, the cells were blocked in PBS containing 3% BSA and 0.1% Triton X-100. The primary antibodies diluted by 1:200 were treated at 4 °C , overnight. The cells were then washed with Tween 20-PBS (PBST), and DyLight488 conjugated donkey anti-rabbit secondary antibodies diluted by 1:200 were applied for 30 min to the cells. This process was performed in a dark place at room temperature. After the cells were washed several times, the coverslips were mounted to slides using a VECTASHIELD mounting media with DAPI (Vector Labs, Burlingame, CA, USA). The images were visualized using an FV500 confocal microscope (Olympus, Tokyo, Japan).

Disease Models
To induce experimental AD, mice were sensitized by applying 1% dinitrochlorobenzene (DNCB; Tokyo Kasei Kogyo Co., Ltd., Tokyo, Japan; 150 μL) or vehicle alone to the abdomen (on Day 7). On Day 0, mice were challenged again by applying 100 μL of 0.3% DNCB to the ears on every other day for up to 30 days. From Day 12 until the completion of the experiment, the mice were painted with hydrocort cream (Green Cross, Yongin, Korea) containing 2 mg/g hydrocortisone valerate and DPHC (10 and 100 mg/kg) to the ears on every other day. The mice were sacrificed on Day 31.

Macroscopic Edema and Histological Evaluation
In the experimental AD mouse model, DNCB stimulation elicited ear edema, and ear thickness was measured using a digital thickness gauge (Mitutoyo, Tokyo, Japan). Ear tissues were fixed with 10% formalin and embedded in paraffin. Paraffin sections (3 μm each) were stained with H & E.

Statistical Analysis
Statistical differences between the groups (experimental and control groups) were analyzed by the Student's t-test. The values were reported as the means and standard deviation (means ± SD) of at least three independent experiments. p-values <0.05, <0.01 and <0.001 were considered to be statistically significant.

Conclusions
DPHC strongly inhibited the production of IL-6 in murine macrophage RAW 264.7 cells. DPHC suppressed the phosphorylation and the nuclear translocation of NF-κB, a central signaling molecule in the inflammation process induced by LPS. Additionally, DPHC inhibited the expression of STAT5 and increased that of SOCS1. Furthermore, TPCK and JI reduced the LPS-induced IL-6 production in RAW 264.7 macrophages. These findings demonstrate that DPHC inhibits IL-6 production via downregulation of the NF-κB and Jak2-STAT5 pathway and upregulation of SOCS1. Furthermore, DPHC alleviated several symptoms (ear edema, lymph node size, serum IgE level, mast cell infiltration) in experimental atopic dermatitis induced in mice. Taken together, DPHC may be a valuable anti-inflammatory compound isolated from marine plants ( Figure 6).