Marine Bromophenol Bis (2,3-Dibromo-4,5-dihydroxy-phenyl)-methane Inhibits the Proliferation, Migration, and Invasion of Hepatocellular Carcinoma Cells via Modulating β1-Integrin/FAK Signaling

Bis (2,3-dibromo-4,5-dihydroxy-phenyl)-methane (BDDPM) is a natural bromophenol compound derived from marine algae. Previous reports have shown that BDDPM possesses antimicrobial activity. In the present study, we found that BDDPM has cytotoxic activity on a wide range of tumor cells, including BEL-7402 cells (IC50 = 8.7 μg/mL). Further studies have shown that prior to the onset of apoptosis, the BDDPM induces BEL-7402 cell detachment by decreasing the adherence of cells to the extracellular matrix (ECM). Detachment experiments have shown that the treatment of BEL-7402 cells with low concentrations of BDDPM (5.0 μg/mL) significantly inhibits cell adhesion to fibronectin and collagen IV as well as cell migration and invasion. High doses of BDDPM (10.0 μg/mL) completely inhibit the migration of BEL-7402 cells, and the expression level of MMPs (MMP-2 and MMP-9) is significantly decreased. Moreover, the expression of β1-integrin and focal adhesion kinase (FAK) is found to be down-regulated by BDDPM. This study suggests that BDDPM has a potential to be developed as a novel anticancer therapeutic agent due to its anti-metastatic activity and also indicates that BDDPM, which has a unique chemical structure, could serve as a lead compound for rational drug design and for future development of anticancer agents.

. The structure of Bis-(2,3-dibromo-4, 5-dihydroxy-phenyl)-methane (BDDPM) and its cytotoxic activity in cancer cell lines. Human cervical cancer cells (Hela), human colon cancer cells (RKO and HCT-116), human hepatoma cells (BEL-7402), and human vascular endothelial cells (HUVEC), as well as human glioblastoma cells (U87) were incubated in the absence or presence of certain concentrations of BDDPM for 24 h at 37 °C. MTT assay was performed to determine the growth inhibition of different cancer cells and HUVEC cells by BDDPM. The experiments were performed more than three times.

BDDPM Inhibits Cancer Cell Proliferation
MTT assays were performed to investigate the effects of BDDPM on the proliferation of six cell lines. As shown in Figure 1B, BDDPM had a significant growth-inhibiting effect on Hela (IC50 = 17.63 μg/mL), RKO (IC50 = 11.37 μg/mL), HCT-116 (IC50 = 10.58 μg/mL), BEL-7402 (IC50 = 8.7 μg/mL) and U87 (IC50 = 23.69 μg/mL) cancer cell lines, and a minimal growth-inhibiting effect on the HUVEC (IC50 = 30.15 μg/mL) cell line. The results indicated that BDDPM had a significant growth-inhibiting effect on the cancer cell lines ( Figure 1B). Among these cell lines, BEL-7402 cells were much more sensitive than the other cell lines. Based on this result, BEL-7402 cells were chosen for the subsequent experiments.

BDDPM Induces Morphological Changes and Apoptosis in BEL-7402 Cell
Morphological changes were observed in cells under an invert microscope. The results showed that the BEL-7402 cells without treatment had the typical characters of human liver cancer cells, most of which were spindle-shaped with smooth edges and firmly attached to the surfaces of the cell culture dish (Figure 2A). However, the cells treated with BDDPM became spherical and detached from the plate surface, and most of them gradually became round with the increase in BDDPM concentration. The number of round cells increased in a dose-dependent manner. Cytoplasmic vacuoles could be detected in the rounded damaged cells under the high power microscope (Figure 2A).
The morphologic changes in the cell membrane were clearly visualized by scanning electronic microscopy (SEM). Remarkable alterations in the cell membrane of BEL-7402 cells were observed after BDDPM treatment. The architecture of untreated BEL-7402 cells displayed a typical polygonal shape ( Figure 2B-I). However, the morphology of cells started to change after incubation with BDDPM. The cells were detached from the substratum, separated from each other, and became spindle-shaped ( Figure 2B-II) after their exposure to 2.5 μg/mL of BDDPM. Membrane bulging and detachment from cytoplasmic inclusion were also observed in the cells with 5.0 μg/mL and 10.0 μg/mL BDDPM treatments (Figures 2B-III and 2B-IV). (A) Cells were seeded in six-well plates (2 × 10 5 cells/well) and allowed to adhere overnight. Then the cells were treated without or with 2.5, 5.0 and 10.0 μg/mL BDDPM for 12 h and subsequently observed under an inverted phase-contrast microscope (magnification, 100×); (B) For scanning electron microscopic observation, the BEL-7402 cells were grown on poly-L-lysine-coated cover slips for 24 h to allow firm attachment and treated with 2.5, 5.0, and 10.0 μg/mL BDDPM for 12 h. Cells were fixed on cover slips coated with gold and analyzed using the KYKY-2800B SEM. Cells were treated with DMSO were considered as negative controls (I). The remaining cells were treated with 2.5 μg/mL (II), 5.0 μg /mL (III) or 10.0 μg/mL (IV) of BDDPM. The BEL-7402 cells treated with DMSO showed a normal smooth surface with a lot of apophysis. In contrast, the cells treated with BDDPM became rounded, and the surface of the cell membrane was markedly disrupted (Scale bar = 10 μm).

BDDPM Induces Apoptosis in BEL-7402 Cells
We further investigated the role of BDDPM in the apoptosis of BEL-7402 cells. Cells were treated with 0, 2.5, 5.0, and 10.0 μg/mL of BDDPM. After being cultured for 24-48 h, cells were collected, and AnnexinV-FITC and PI staining assays were performed to quantify the number of apoptotic cells. As shown in Figure 3A, BDDPM exposure resulted in an increase in the number of early apoptotic cells (AnnexinV-FITC-positive/PI-negative) in BEL-7402 in a dose-dependent manner. When treated with BDDPM at 2.5 and 10.0 μg/mL, the percentage of apoptotic cells was increased from 25.68% to 87.47%, respectively ( Figure 3B). Subsequently, Hoechst 33342 staining was also performed to detect the apoptotic cells. Cell nuclear pyknosis, chromosome condensation and formation of apoptotic bodies were observed in BEL-7402 cells treated with BDDPM as detected by Hoechst 33342 staining. However, no apoptosis was found in cells treated with DMSO ( Figure 3C). Cleavages of Caspase 3, 9, and poly ADP ribose polymerase (PARP) are important events for the activation of the intrinsic apoptotic pathway. Western blot analysis was performed to determine if BDDPM treatment resulted in cleavages of Caspases and PARP. The results showed that BDDPM promoted the cleavages of Caspase 3, 9, and PARP expression in a dose-dependent manner ( Figure 3D). These results suggest that BDDPM induces cell death via the intrinsic apoptotic pathway.

BDDPM Affects the Migration and Invasion of BEL-7402 Cells
Cell migration and invasion play crucial roles in tumor metastasis [11,18,20]. To further investigate the anti-metastatic effect of BDDPM, the ability of BEL-7402 cell migration was assessed by scratch-wound assay and transwell assays. The results from the scratch-wound assay showed that wound healing gradually reduced as the concentration of the BDDPM increased ( Figure 4A). We next investigated the anti-invasion activity of BDDPM on BEL-7402 cells using a transwell system. As shown in Figure 4B, treatment of BEL-7402 cells with BDDPM significantly inhibited the invasion of the cancer cells in a dose-dependent manner. When BEL-7402 was exposed to BDDPM at a concentration of 2.5, 5.0 and 10.0 μg/mL, the cell invasion to transwell was inhibited by 47.8%, 70.7%, and 86.2%, respectively ( Figure 4B,C). These results suggested that BDDPM affected the ability of cell migration and invasion.
Both of the above findings indicated that BDDPM could significantly prevent BEL-7402 migration and invasion. Since inhibition of cell migration by BDDPM occurred before its inhibitory effect on cell proliferation was observed, the results suggest that BDDPM might indeed affect BEL-7402 cell migration and invasion, regardless of its effect on cell proliferation.

BDDPM Inhibits the Ability of BEL-7402 Cells to Adhere to ECM
It is well known that some extracellular matrix (ECM) proteins, such as collagen IV, fibronectin (FN), and laminin (LN) play an important role in cell adhesion. To determine whether BDDPM affects some molecular events associated with cell attachment. The anti-adhesion effect of BDDPM on BEL-7402 cells was assessed by testing the adhesion ability of the cells to a cell matrix containing Col IV, FN, or LN. As shown in Figure 5, BDDPM remarkably reduced the adhesive ability of BEL-7402 cells to Col IV, FN or LN. Approximately 86.74% reduction in the number of cells adhering to Col IV gel was detected under the treatment of BDDPM (5.0 μg/mL), while exposure to the same concentration of BDDPM led to an adhesion of the BEL-7402 cells to the FN-containing matrix and a reduction of LN by 70.31% and 61.23%, respectively. However, BDDPM did not inhibit BEL-7402 cell adhesion to poly-L-lysine (p > 0.05), a non-ECM matrix. These results demonstrate that the treatment of BEL-7402 cells with BDDPM could inhibit the ability of these cells to adhere to ECM and result in cell detachment. proteins. Bel-7402 cells were suspended in serum-free medium containing 0.2% BSA without or with 5.0 μg/mL BDDPM and then seeded into pre-coated 96-well plates with 2.5 μg/mL fibronectin (FN), laminin (LN), poly-L-lysine (PL) or 5.0 μg/mL collagen IV (Col IV), respectively, and allowed to adhere for 1 h at 37 °C. After washing with PBS, the adhering cells were measured using an MTT assay. The adhesion rate of the treated cells was normalized to the control group. Data is shown as Mean ± SD from three independent experiments. ** p < 0.01 vs. control.

BDDPM Disrupts the Cytoskeleton and Changes the Morphology of BEL-7402
The effect of BDDPM on F-actin cytoskeleton organization was examined by immunofluorescence. As shown in Figure 6, BDDPM led to a dramatic disruption of the BEL-7402 cell cytoskeleton, producing a diffuse microtubule network and an increase in actin stress fibers and membrane blebbing.
At the same time, cell morphology was significantly changed, with a rounded and retracted shape following exposure to BDDPM ( Figure 6).

Figure 6.
Effects of BDDPM on the BEL-7402 cell cytoskeleton. Human BEL-7402 cells were seeded onto cover slips coated with fibronectin and incubated over night prior to treatment (12 h, with or without 5.0 μg/mL BDDPM). Cells were then fixed and stained for F-actin (red) and the nuclei of the cells were stained using 4′,6-diamidino-2-phenylindole (DAPI) (blue); fluorescence images were viewed using a Zeiss confocal microscope (20×).

BDDPM Inhibits the Expression of β1-Integrin and FAK
To investigate the possible molecular mechanism underlying the effects of BDDPM on BEL-7402 cell behaviors, we performed flow cytometry and Western blot analysis to detect the expression of β1-integrin. Flow cytometrical analysis showed that, when the cells were treated with 5.0 μg/mL of BDDPM, the β1-integrin expression on the cell surface was significantly down-regulated in a dose-dependent manner ( Figure 7A) compared to control cells. Accordingly, FAK, which is activated by β-integrin in normal and cancer cells, was significantly inhibited by BDDPM. Exposure to BDDMP resulted in the decrease in both total FAK protein and the activated FAK (phosphorylated FAK) expression levels ( Figure 7A). Next, we detected the expression levels of MMP-2 and MMP-9, which are regulated by FAK and are critical for cancer cell invasion. The results revealed that treatment with BDDPM resulted in a significant decrease in the expression levels of MMP2 and MMP-9 in a dose-dependant manner ( Figure 7B). PI3K/Akt and ERK are also in the downstream cascades of FAK signaling, and FAK phosphorylation of Akt/ERK suggests Akt and ERK activation. In the present study we examined the effects of BDDPM on Akt/ERK phosphorylation using antibodies recognizing phospho-serine 473 of Akt and phospho-Thr202/Tyr204 of ERK. The cells were incubated for 1 h in a medium containing 2.5-10.0 μg/mL of BDDPM. Drug treatment decreased the normalized levels of phospho-Akt and phospho-ERK in BEL-7402 cells ( Figure 7C). This observation demonstrated that BDDPM inhibition of FAK kinase activity could decrease Akt and ERK activity. These results indicate that BDDPM inhibits proliferation, migration, and invasion of BEL-7402 cells by disturbing the β1-integrin/FAK signaling pathway. The anti-phospho-AKT antibody and anti-phospho-ERK antibody were used. The same membrane was stripped and re-probed using the anti-AKT, anti-ERK or anti-GAPDH antibody to detect total AKT and ERK levels. The cells treated with DMSO were used as negative control and the expression of GAPDH was used as a loading control.

Discussion
Tumor metastasis is a multistep process that involves tumor cell detachment from the primary tumor, adhesion to ECM or basement membrane, migration, invasion, angiogenesis, and metastatic tumor cell growth [20]. Tumor metastasis is also a major cause of death of cancer patients, and its blockage has been considered to benefit the survival of cancer patients [21]. Thus, it is crucial to identify new promising agents with anti-metastatic activity, which can disrupt or block metastasis. In this study, we found that the natural product BDDPM has antitumor activity on many types of cancer cells, and it has a potential to be developed as an anti-metastasis agent. Several lines of evidence suggest that BDDPM has anti-metastasis activity in vitro. Firstly, BDDPM has been found to induce cancer cell detachment and cause their apoptotic death. Secondly, BDDPM inhibits BEL-7402 cell adhesion to ECM and the major ECM proteins FN, LN, and Col IV. The promotion of detachment and inhibition of adhesion in BEL-7402 cancer cells mediated by BDDMP indicates that it disrupts the dynamic balance between attachment and detachment of cancer cells, whereas such dynamic balance regulates cell motility and is a fundamental premise for the metastasis of cancer cells [22,23]. It is assumed that BDDMP possesses anti-metastasis activity in vitro. Thirdly, wound a healing assay and a Boyden chamber assay revealed that BDDMP inhibites the migration and invasion of BEL-7402 cancer cells. Thus, BDDMP possesses anti-metastasis activity in vitro and could be developed as a novel anticancer agent.
Integrins are the major cell adhesion receptors that mediate cell adhesion to ECM proteins and influence diverse cellular functions crucial for the initiation, progression, and metastasis of solid tumors [24]. The importance of integrins for tumor progression has made them an appealing target for cancer therapy. In recent years, various integrin antagonists or inhibitors have been developed. Cilengitide, an inhibitor of both αvβ3 and αvβ5 integrins, has been tested in Phase II trials in patients with lung and prostate cancer [25], and Phase II and Phase III trials are currently underway for glioblastoma treatment [26][27][28]. Volociximab, a function-blocking antibody against σ5β1 integrin, is currently undergoing Phase II clinical trials for solid tumors [29]. In the present study, we also found that BDDMP had a direct effect on the expression of β1-integrin ( Figure 6). The β1-integrin is a candidate target well known for mediating cell-ECM interactions. Recent studies have shown that its aberrant expression is implicated in cancer progression and the resistance to cytotoxic therapy [30]. With the down-regulation of β1-integrin by siRNA or miR-134, the cell adhesion and invasiveness were highly inhibited [31,32]. Our findings indicate that BDDPM could be developed into a novel inhibitor of β1-integrin. However, further studies are needed to verify BDDPM-induced down-regulation of β1-integrin in BEL-7402 cancer cells, as well as in other cancer cell lines. A study is in progress in our laboratory to investigate if the treatment of cancer cells with BDDPM affects the expression of other types of integrins.
FAK activation and phosphorylation stimulated by integrins is critical for anchorage-independent growth of cancer cells. Several studies have revealed that increased FAK expression is correlated with enhanced tumor malignancy and poor prognosis [33]. Recent studies that employed RNAi to inhibit FAK expression in carcinoma cells have been yielding insights into FAK's role for tumor growth and spreading, and have also revealed that FAK expression and activity are essential for cell invasiveness [12]. FAK is required for cell transformation and invasion [13,23,34], which has become an attractive target for drug discovery. To determine the effects of BDDPM-inhibited FAK on downstream signaling and cell phenotypes, the important downstream genes including AKT/ERK were also investigated in the present study, and we found that BDDPM inhibition of FAK activity in BDDPM cells resulted in a lower AKT/ERK phosphorylation/activity, which is correlated with decreased survival and increased apoptosis (cleaved PARP and Caspases increased). Besides, BDDPM inhibits cell migration by downregulation of the MMP-2 and MMP-9. All the results indicate that BDDPM targets FAK kinase activity and inhibits FAK-related AKT/ERK activation, which impacts cell viability, and decreases anchorage-independent growth and motility. In summary, the present study found that BDDPM could disturb the integrin-FAK signal transduction and that the FAK expression level was decreased by BDDPM treatment, indicating that BDDPM has a potential to be developed as an intergrin-FAK inhibitor.

Materials
BDDPM was first isolated from Rhodomelaceae confervoides, and was subsequently synthesized by our lab (purity, 98%) [9]. The Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and other cell culture reagents were purchased from Invitrogen (Carlsbad, CA, USA). The high concentration ECM gel was purchased from BD Biosciences (BD Biosciences, Bedford, MA, USA).

MTT Assay
MTT assay was performed as previously reported [35]. Briefly, cells were seeded in 96-well plates (5 × 10 3 cells/well) and allowed to adhere overnight. After the cells were treated with specific doses of samples for 24 h at 37 °C, a MTT solution (30 μL, 5 mg/mL) was added into each well and the plates were incubated for 4 h before the MTT-containing solution was removed and replaced with 150 μL of DMSO. The absorbance at 490 nm was then determined with an ELx808 microplate reader (BioTek, Winooski, VT, USA). The viability rate of the treated cells was calculated by the formula: cell viability rate (%) = [(A490 sample)/(A490 control)] × 100%. The IC50 value was deduced from the MTT dose-response curves of cell viability against drug concentration.

Cell Morphological Observation
Morphologic alterations in BEL-7402 cells after BDDPM treatment were observed and photographed under an inverted microscope (Carl Zeiss, Oberkochen, Germany) or scanning electron microscope (SEM). For inverted microscope observation, cells in logarithmic phase were suspended in 1640 medium with 10% FBS and seeded into 24-well plates (50,000 cells/well). After incubation for 24 h, cells were treated with BDDPM (0, 2.5, 5.0, 10.0 μg/mL) and cultured for 12 h. Cell morphological changes were observed and photographed under an inverted microscope (Carl Zeiss, Oberkochen, Germany). For scanning electron microscope assay, the cells were grown on poly-L-lysine-coated coverslips in six-well plates for 24 h to allow firm attachment. Cells were then treated with 2.5, 5.0, 10.0 μg/mL BDDPM and incubated for 12 h. The medium containing BDDPM was removed, and the cells were subsequently fixed in 0.25% glutaraldehyde. After fixation overnight at 4 °C, the coverslips were dehydrated with ethanol and dried in a critical point dryer. Cells on cover slips were coated with gold and analyzed with the S-3400N SEM (Hitachi, Lexington, KY, USA).

Hoechst33342/Propidium Iodide (PI) Dual Staining Assays
The apoptotic cells were stained using Hoechst33342/PI double staining as we described previously [35]. The BEL-7402 cells were seeded in six-well plates (2 × 10 5 cells/well) and treated with certain concentrations of BDDPM (2.5, 5.0, 10.0 μg/mL). After incubation for 24 h, the cells were collected and washed with PBS. Cells were stained with Hoechst33342 and PI using the dual staining kit (Beyond, Beijing, China). Then the cells were spread on slides and observed under the fluorescence microscope (Carl Zeiss, Oberkochen, Germany).

Apoptosis Assay
Cell apoptosis was detected by Annexin V-FITC assay. Apoptotic cell death was quantified by flow cytometry with Annexin V-FITC and propidium iodide (PI) staining. Annexin V-FITC apoptosis detection kit (Invitrogen, Carlsbad, CA, USA) was used according to the manufacturer's instructions. Briefly, both attached and floating cells were collected and resuspended in binding buffer before adding the Annexin V-FITC antibody and PI. Stained cells were analyzed by flow cytometry (Beckman Coulter,Brea, CA, USA).

Cell Adhesion Assay
The cell adhesion assay was performed as described previously [19]. Briefly, Bel-7402 cells were suspended in serum-free medium containing 0.2% BSA without or with 5 μg/mL BDDPM and then seeded in precoated 96-well plates with 2.5 μg/mL fibronectin (FN) and laminin (LN), poly-L-lysine (PL) or 5 μg/mL collagen IV (Col IV), respectively, and allowed to adhere for 1 h at 37 °C. After washing with PBS, the adherent cells were measured using an MTT assay. The adherent rate of the treated cells was normalized to the control group.

Cell Migration Assay
The migration ability of BEL-7402 cells was assessed using a modified version of a previously described protocol [34] with a transwell system (Corning, Tewksbury, MA, USA). Cells (10,000 cells/well) were added to the upper chamber of the transwell culture plates, in the presence and absence of certain concentrations of BDDPM (0.0-10.0 μg/mL). The lower chamber was filled with 500 μL F-12K medium containing 10% FBS as a chemo-attractant. After incubation for 12 h, non-migrating cells on the upper surface of the membrane were scrubbed gently with a cotton-tipped swab. The migratory cells on the lower surface of the membrane were fixed with 95% methanol, stained with 0.1% crystal violet (Sigma-Aldrich, St. Louis, MO, USA), counted with inverted microscope and quantified by manual counting in three randomly selected areas.

Cell Invasion Assay
The effect of BDDPM on BL-7402 cell invasion was measured by a transwell system with a diameter of 6.5 mm and a pore size of 8 μm. ECM gel was applied to the top side of the filter to form a thin gel layer. As described above for the cell migration assay, cells that penetrated to the lower chamber were fixed and stained. The stained invasive cells were photographed under an inverted light microscope and quantified by manual counting in three randomly selected areas.

Cytoskeleton Immunofluorescence
BEL-7402 cells were seeded on ploy lysine-coated chamber slides before exposure to BDDPM (10 μg/mL) for 4 h. The cells were then harvested and fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with 5% BSA solution. The microtubules were then labeled with mouse monoclonal anti-F-actin antibody (1:500, Santa Cruz, Dallas, TX, USA), followed by incubated with the second antibody (Alexa Fluor549-anti-Rabbit IgG, 1:1000, Life science, St. Louis, MO, USA), after that the cell nuclei were stained with DAPI (5 mg/mL) for five minutes and the Fluorescence images were obtained by using confocal microscope (Zeiss, Oberkochen, Germany).

Western Blot Analysis
Cells were lysed in RIPA buffer (Solaibo, Beijing, China). Proteins were separated by a 10% polyacrylamide gel and transferred to a methanol-activated PVDF membrane (GE Healthcare, Little Chalfont, Buckinghamshire, UK). The membrane was blocked in blocking solution (5% nonfat dry milk powder) for 2 h at room temperature and subsequently probed with primary antibodies; including ILK, β1-integrin, PARP, Caspase-3, Caspase-9, total/phospho FAK, total/phospho-Akt, total/phospho-ERK, (used at a 1/1000 dilution, Santa Cruz Biotechnology, Santa Cruz, CA, USA), MMP2, MMP9 (used at a 1/1000 dilution, AbClonal, USA) Or GAPDH (used at a 1/5000 dilution, AbClonal, Cambridge, MA, USA). After three 10 min washes with 0.1% Tween-20 in PBS buffer, membranes were incubated with rabbit anti mouse or goat anti rabbit HRP-conjugated secondary antibody (Santa Cruz) for 1 h. After an additional three 10 min washes with 0.1% Tween-20 in PBS buffer, the chemiluminescence method was employed to detect the signals using Super Signal West Dura (Thermo Scientific, Waltham, MA, USA) and protein bands were visualized by autoradiography.

Statistical Analysis
Statistical significance of the data was analyzed by the two-tail Student's t-test with a minimum significance level set at p < 0.05 (marked as * p < 0.05 and ** p < 0.01).

Conclusions
This study suggests that the natural marine bromphenol compound Bis (2,3-dibromo-4,5-dihydroxyphenyl)-methane (BDDPM) induces cancer cell detachment and causes their apoptotic death, and it has a potential to be developed as a novel anticancer therapeutic agent due to its anti-metastatic activity.

Acknowledgments
This study was supported by the National Natural Science Foundation of China (41306157, 41276167 and 41206066) and Basic research projects of Qingdao Science and Technology plan (12-1-4-8-(4)-jch).