Paper Synthesis, Cytotoxicity and Apoptosis Induction in Human Tumor Cells by Galaxamide and Its Analogues

Our previous study reported that galaxamide, which is a cyclo-pentapeptide containing five leucines that was extracted from Galaxaura filamentosa, displayed remarkable anticancer cytotoxicity. This novel cyclo-peptide provided a new skeleton for the structural modifications used in finding new drugs with better anticancer properties. In this study, five analogues were synthesized based on changing the number of d/l amino acids by adding a new amino acid, phenylalanine. Galaxamide and five of its analogues were evaluated through MTT assays to examine their cytotoxic activities. We found that modified analogue 5, which is referred to as A5, displayed broad spectrum cytotoxic activity toward every cell line tested; in addition, the IC50 of A5 was lower than that of galaxamide and the other analogues. Furthermore, we used flow cytometry and western blot assays to investigate whether galaxamide and A5 could induce cancer cell apoptosis. The flow cytometric studies showed that HepG2 cells treated with different concentrations of galaxamide or A5 over 72 h displayed significant and dose-dependent increases in the percentages of early-stage apoptotic cells. Western blotting revealed that both compounds induce caspase-dependent apoptosis in HepG2 cells through a mitochondria-mediated pathway. The results demonstrate that galaxamide and its analogues have potential applications as clinical anticancer drugs.


Introduction
The ocean has more species diversity than land, supplying numerous organisms with chemical properties that are different from their terrestrial counterparts [1]. Cyclic peptides are an important class of marine compounds that may exhibit strong antitumor, antiviral and antibiotic activities [2]. Many structurally novel bioactive cyclopeptides have been isolated from marine cyanobacteria in recent decades [3]. Cyclic peptides tend to have a greater binding affinity for protein targets than their linear counterparts or small molecules, because their bond rotation is restricted and their structures are conformationally constrained [4]. Galaxamide is a novel cyclic pentapeptide isolated from Galaxaura filamentosa in the South China Sea, Xisha Islands. Our earlier research resulted in the isolation, structural determination and total synthesis of galaxamide, revealing significant antitumor activity against the human renal cell carcinoma GRC-1 and hepatocellular carcinoma HepG2 cell lines [5]. The pharmacological and toxicological profiles of galaxamide strongly suggested that galaxamide might be a promising anticancer drug candidate. Therefore, we modified galaxamide and investigated its structure-activity relationship to discover novel antitumor agents among the galaxamide analogues. In this work, we changed the number of D-amino acids in galaxamide [6] and different residues, including leucine and phenylalanine, which is considered an active group in cyclopentapeptide [7], were replaced to synthesize analogues 1-5 (referred to as A1-A5, Figure 1). All of the compounds were evaluated for their cytotoxicity against four human cancer cell lines, HepG2, MCF-7, SW480 and U87, as well as normal human liver cells (L02). As expected, several modified compounds showed strong antitumor activities; in particular, A5 demonstrated stronger cytotoxic activities than galaxamide against all of the tested cancer cell lines. Furthermore, we demonstrated that galaxamide and A5 could activate caspase-3, caspase-9 and PARP, inducing apoptosis in HepG2 and elucidating the mechanism of its anti-tumor activity.

Chemistry
In our previous paper, we described the synthesis of galaxamide, which is a cyclic peptide [5]. We employed the same solution phase method to synthesize galaxamides A1-A5, as shown here (Scheme 1) for A5.

Galaxamide and Its Analogues Inhibit the Proliferation of Different Cancer Cells
To examine the effect of galaxamide and its five analogues on cell proliferation, four cancer cell lines and one normal cell line, including human hepatocellular carcinoma HepG2, human breast carcinoma MCF-7, human colon carcinoma SW480 and human glioma cell line U87, as well as human normal liver cell line L02, were exposed to all of the compounds at different concentrations before being subjected to an MTT assay. Galaxamide and A1-A5 were cytotoxic toward different cancer cell lines while exhibiting low cytotoxicity toward normal liver cell line L02. All of the tested compounds exhibited strong cytotoxic activity against HepG2 cells. When comparing galaxamide and its five analogues, A5 demonstrated the highest cytotoxicity against every cell line. The results are summarized in Table 1 and discussed below.

Effect of Galaxamide and A5 on the Cell Morphology and Nuclear Integrity
Galaxamide and A5 induced morphological changes in HepG2 cells. Treating HepG2 cells with 3, 6, 12 μg/mL of galaxamide and A5 for 72 h resulted in the detachment of most cells from the tissue culture plates and cell death. These behaviors might be caused by cytotoxicity toward the cells. The number of cells detached from the culture plates increased when increasing the concentrations of galaxamide and A5 in the plates. To examine whether the cells died through apoptotic mechanisms, the control and the galaxamide-and A5-treated cells were stained with Hoechst33342. The resulting morphological changes were observed under a fluorescence microscope ( Figure 2). The control cells exhibited normal morphologies with round nuclei. However, the galaxamide-and A5-treated cells showed chromatin condensation at the edges of the nuclear membrane and nuclear fragmentation. As the concentration of galaxamide and A5 was increased to 12 μg/mL, most of them were stimulated to death, but morphological changes still could be observed.

Galaxamide and A5 Activate Caspase-3, Caspase-9 and PARP in HepG2
Based on the increased apoptosis of galaxamide-and A5-treated cells, we next examined whether caspase-3, caspase-9 and PARP activities were changed after treatment with galaxamide and A5. As shown in Figure 4, the cleaved caspase-3, caspase-9 and PARP expressions were significantly increased after their treatment (3, 6, 12, and 24 μg/mL) for 72 h. These findings suggest that galaxamide and A5 might induce apoptosis by regulating mitochondria-mediated pathways.  . The expression of apoptosis-associated proteins in HepG2 cell treated with galaxamide and A5 for 72 h. A western blot was used to analyze whole cell lysates for caspase-3, caspase-9 and cleaved PARP expression after treatment with galaxamide and A5, and equal amounts of the protein were loaded onto each lane. One representative immunoblot from three independent experiments is presented. The fold change was calculated as the ratio of cleaved caspase-3, cleaved caspasse-9 and cleaved PARP in the different concentration of Galaxamide or A5 to their expression in the control cells, after normalization to tubulin in each lane. All data shown are representative of at least three separate studies.

Discussion
In our previous paper [5], we described the isolation, structural determination and synthesis of galaxamide. In the present study, galaxamide and analogues A1-A5 were synthesized; they all exhibited significant anti-tumor activity against different cancer cells. Furthermore, our data demonstrated that galaxamide and the best cytotoxic analogue, A5, could induce cell apoptosis through a mitochondria-mediated apoptosis pathway. Galaxamide and its analogues might be anti-tumor therapeutic agents ready for further study.
Galaxamide and A1-A5 were tested for inhibitory activity against the growth of several tumor cell lines and one normal liver cell line. As shown in Table 1, some modified compounds exhibited much better activity than galaxamide. Notably, A5 showed broad-spectrum antitumor activity against HepG2, SW480, U87 against MCF-7 cell lines with IC 50 values of 1.46, 5.1, 1.85 and 4.18 μg/mL, which were 3.17-, 8.59-, 5.73-and 3.37-fold more active than galaxamide (with IC 50 value of 4.63, 43.82, 10.61 and 14.09 μg/mL), respectively. Galaxamide and A1-A4 exhibited low cytotoxic activity toward normal liver cell line L02 (IC 50 > 40 μg/mL). Although A5 achieved an IC 50 value of 10.55 μg/mL, this value was about tenfold higher than that of HepG2. Galaxamide and its analogues affect cancer cells differently than normal cells. Moreover, all of the cyclopeptide exhibited strong antiproliferative activity against HepG2 cell, revealing that galaxamide and its analogues might be more effective against HepG2 than other types of tumor cells. Both A1 and A2 displayed better cytotoxic activities against HepG2, MCF-7 and SW480. A3 and A4 did not exhibited equal cytotoxic activity against HepG2 compared to galaxamide, but they performed better than galaxamide against SW480 and MCF-7; in addition, A4 had an IC 50 value of 2.82 μg/mL and was more active than galaxamide.
Relative to the structure, A5 has four D-amino acids in Positions 2-5. In comparison, A1 has phenylalanine, while A2 is changed into a D-amino acid at Position 1. A3 has two D-amino acids in Positions 2 and 4, and A4 has three D-amino acids in Positions 1, 2 and 4. The structural analysis revealed that: (1) A1 and A2, which contain D/L-phenylalanine, exhibited favorable activity; (2) A5 was more potent than the other analogues, suggesting that the compounds with four D-amino acids, including a D-N-Me-phenylalanine, exhibit better activity than analogues bearing fewer D-amino acids than A2-A4. Future analogues that incorporate additional D-amino acids at the other positions will be synthesized and tested.
Apoptosis is an important homeostatic mechanism that balances cell division and death while maintaining the appropriate number of cells in the body [14]. Many therapeutic peptides, such as aplidine and didemnin B, which both were isolated from marine animals, could induce apoptosis in cancer cells [15,16]. Cyclopentapeptide sansalvamide A is a derivative of sansalvamide A, which is a depsipeptide with antitumor activity that promotes apoptosis in cancer cells [17]. Galaxamide and its analogues were also peptide compounds. In the present study, Annexin V/PI double-staining assays revealed that galaxamides and A5 induced apoptosis in HepG2 cells. Therefore, inducing apoptosis might be the primary mechanism for the anti-cancer activity of galaxamides. In addition, a lower concentration of A5 induced a better apoptosis effect than galaxamides. Similar to the results of the cytotoxicity experiments, we believe that A5 has stronger anti-cancer activity.
Apoptosis can be induced through two distinct pathways [18]: the ligation of the TNF/Fas-receptor with its ligand followed by caspase-8 activation to activate caspase-3 directly and the mitochondria-mediated caspase-9 activation pathway. Initiator caspases cleave procaspases are able to activate effector caspases (caspase-3, -6 and -7) or amplify the caspase cascade by increasing the activation of the initiator caspases. Both pathways converge at caspase-3, leading to cell death. In the present study, caspase-3, caspase-9 and PARP were all activated in HepG2 after being treated with galaxamide or A5. Therefore, the apoptosis of HepG2 induced by galaxamide and A5 might be related to a mitochondria-mediated pathway.
In summary, galaxamide and its analogues have potent antitumor effects and cause cell apoptosis in vitro. Future analogues that incorporate additional D-amino acids at other positions will be synthesized and tested. In addition, improving the yield of the cyclization is also necessary.

Chemistry
The reagents and solvents were commercially available. The solvents were dried and purified through standard procedures before use. The NMR spectra were obtained on a Varian Inova-500 NB spectrometer in CDCl 3 , while using TMS as an internal standard; the coupling constants (J) are in Hz. The ESI mass spectra were obtained on a LCQ DECA XP LC-MS mass spectrometer. Column chromatography was performed using silica gel (Qingdao Ocean Chemical Factory, Qingdao, China, 200-300 mesh) eluted with petroleum ether/acetone or n-hexane/acetone; C18 reversed phase silica gel (Welch Material Inc., Shanghai, China, 45 μm) was eluted with methanol-water.

Observations of Nuclear Damage
Exponentially growing HepG2 cells (1 × 10 5 cells/mL) were incubated for 72 h with 3, 6, 12 μg/mL of galaxamide and A5, respectively. Apoptotic nuclear morphology was visualized by the Hoechsst33342 staining technique. Cells were fixed with 3.7% of paraformaldehyde for 10 min at room temperature and washed three times with PBS. Thus, paraformaldehyde-fixed cells were stained using Hoechsst33342 (10 μg/mL) in the dark for 10 min. After washing three times with PBS, cells were visualized using fluorescence microscope.

Western Blot Analysis
The protein was extracted with RIPA buffer and quantified using the Bradford method. Equal amounts of protein were loaded into each well. After SDS-PAGE, the proteins were transferred to a polyvinylidene difluoride (PVDF) membrane. The membrane was blocked with 5% non-fat milk for 1 h and incubated with a primary antibody (Cell Signaling Technology, Danvers, MA, USA, Rabbit monoclonal antibody, 1:1000) at 4 °C overnight. After washing two times with TBST (Tris-buffered saline with 0.1% Tween-20) buffer, the membrane was incubated with a secondary antibody (goat-anti-rabbit horseradish peroxidase (HRP)-conjugated 1:15,000) at room temperature for 1 h. The membranes were washed three times (8 min each time) with TBST. The intensity of the specific immunoreactive bands was detected through enhanced chemiluminescence (ECL).

Statistical Analysis
The data are expressed as the mean ± standard error of mean (SEM). The statistical comparisons were performed using the Student's t-test, and the differences between groups were considered significant at p < 0.05.

Conclusions
This paper has reported the synthesis and the biological evaluation of novel cyclopeptides, including galaxamide and its analogues A1-A5, against a series of cancer cells and normal liver cells. Some modified analogues exhibited strong cytotoxicity against cancer cells. Meanwhile, galaxamide and A5 activated an apoptotic pathway involving the sequential activation of caspases 9, 3 and PARP in the HepG2 cells. Interest in exploring the chemical structure relationship of galaxamide and analogues with anti-tumor properties for new drug design has grown.