A New Benzofuran Glycoside and Indole Alkaloids from a Sponge-Associated Rare Actinomycete, Amycolatopsis sp.

Three new secondary metabolites, amycofuran (1), amycocyclopiazonic acid (2), and amycolactam (3), were isolated from the sponge-associated rare actinomycete Amycolatopsis sp. Based on combined spectroscopic analyses, the structures of 1–3 were determined to be a new benzofuran glycoside and new indole alkaloids related to cyclopiazonic acids, a class that has previously only been reported in fungi. The absolute configurations of 1 and 3 were deduced by ECD calculations, whereas that of 2 was determined using the modified Mosher method. Amycolactam (3) displayed significant cytotoxicity against the gastric cancer cell line SNU638 and the colon cancer cell line HCT116.


Introduction
Natural products from marine organisms have been proposed as "a new wave of drugs" because of their great potential for supplying structurally and biologically novel compounds for drug discovery [1]. As of 2013, several drugs derived from marine natural products are in clinical use. These drugs include the anticancer drug cytarabine and the antiviral agent vidarabine from the sponge Tethya crypta; Prialt from the cone snail Conus magnus, which is used for chronic pain treatment; the anticancer drug Yondelis from the marine tunicate Ecteinascidia turbinata; and Halaven from the sponge Halichondria okadai, a compound used to treat breast cancer [2]. During drug development, compound supply was one of the most challenging problems to overcome, as these drugs originate from marine invertebrates, which are difficult to cultivate [1]. The recent advent of the genomic era and the development of microbial biotechnology have revealed that the majority of secondary metabolites from marine invertebrates are actually produced by symbiotic microorganisms in invertebrate tissues, indicating that the microbial fermentation of useful marine invertebrate-derived compounds may resolve the supply problem [3]. For example, a recent study determined that didemnin anticancer agents, originally discovered from the tunicate Trididemnum solidum, were produced by the symbiotic bacteria Tistrella mobilis and Tistrella bauzanensis [4]. The sponge-derived metabolites swinholide A [5,6], onnamide A [7], and psymberin [8] were also shown to be bacterially produced natural products. These results have spurred the study of the chemistry and phylogenetic diversity of symbiotic microbial communities in marine invertebrates [9,10].
Actinomycetes, belonging to the phylum Actinobacteria, are prolific chemical synthesizers, providing 40% of the 33,500 microbial bioactive compounds reported as of 2010, including numerous drugs in clinical use [11]. Actinomycetes are one of the major phylogenetic groups in symbiosis with sponges by accounting for ~15% of all symbiotic communities associated with sponges [9]. Given that actinomycetes are chemically prolific and that sponge-associated actinomycetes have not been thoroughly studied, chemical investigations of sponge-associated actinomycetes should lead to the discovery of a novel chemical hemisphere [12]. However, compared to the phylogenetic analysis of sponge-associated microbial communities, the chemical investigation of sponge-associated actinomycetes is only just beginning, as demonstrated by a few recent examples, including new nucleoside analogs discovered from Streptomyces microflavus in association with the sponge Hymeniacidon perlevis [13] and new lobophorin derivatives isolated from a sponge-associated actinomycete, Streptomyces carnosus [14]. Therefore, we selectively isolated actinomycete strains associated with marine sponges to search for new bioactive compounds. Then, we chemically analyzed the production of secondary metabolites from sponge-associated actinomycetes by LC/MS (Liquid Chromatography/Mass Spectrometry). During our chemical screening, we found that a rare actinomycete strain (Cra33g) belonging to Amycolatopsis sp., produced a new benzofuran glycoside, as well as two new indole alkaloids, which are quite rare as bacterial metabolites. Here, we report the structural elucidation and biological activity of these three new compounds from the Amycolatopsis strain: amycofuran (1), amycocyclopiazonic acid (2), and amycolactam (3) (Figure 1).

Structural Elucidation
Amycofuran (1) 11.07, 7.07, 6.70, and 6.87). The 13 C NMR spectrum revealed ten double-bond carbons, including four quaternary carbons between 154.1 and 130.7 ppm, one dioxygenated carbon at δ C 101.5, five oxygenated sp 3 carbons from 84.5 to 71.4 ppm, an aliphatic carbon at δ C 37.8, and two methyl carbons (δ C 18.9 and 18.5). Further analysis of the 1 H-1 H COSY (Correlation Spectroscopy) NMR spectrum was useful for the construction of two separate spin systems. First, a diene-bearing system was elucidated by the consecutive COSY correlations from H 2 -7 (δ C 3.27 and 2.95) to H 3 -13 (δ C 1.63) through H-8, H-9, H-10, H-11, and H-12 ( Figure 2). The geometries of the diene were established as 9E and 11E based on the trans-coupling constants (15.0 Hz each) of the double-bond 1 H peaks corresponding to H-9, H-10, H-11, and H-12. The second spin system indicated the presence of a hexose. The proton bound to a dioxygenated carbon (H-1′; δ H 6.30-δ C 101.5) was correlated with the carbinol proton H-2′ (δ H 4.82) in the COSY spectrum, revealing C-1′-C-2′ connectivity. H-2′ displayed a homonuclear coupling with H-3′ (δ H 4.75), placing C-3′ next to C-2′. The COSY correlation between H-3′ and H-4′ (δ H 4.35) connected C-3′ and C-4′. Further extension of the spin system was accomplished using the COSY correlations between H-4′ and H-5′ (δ H 4.52) and between H-5′ and H 3 -6′ (δ H 1.56), suggesting a hexose. The hydroxy protons (δ H 7.07, 6.70, and 6.87) belonging to the hexose were assigned based on COSY correlations.   The analysis of the HMBC (Heteronuclear Multiple Bond Correlation) correlations allowed complete elucidation of the planar structure of amycofuran (1). The long-range heteronuclear coupling from H-5' to the dioxygenated carbon at 101.5 ppm confirmed the identity of the sugar unit as a six-membered ring. The HMBC correlations from the meta-coupled aromatic protons (H-3 at  H 7.25; H-5 at  H 6.83) to the aromatic carbons C-1 to C-6 established a six-membered aromatic ring. The connectivity of the diene-bearing spin system to the aromatic ring was confirmed by the HMBC correlations from H 2 -7 to C-1, C-5, and C-6. An additional long-range coupling from H-8 to C-1 indicated that the dihydrofuran ring was attached to the aromatic ring. The HMBC correlation from H-1′ to C-2 connected the sugar moiety to C-2, completing the planar structure of amycofuran (1) and indicating a new benzofuran. The relative configuration of the hexose was examined using the 1 J CH value of the anomeric proton and the 3 J HH values and ROESY (Rotating-frame Overhauser Effect Spectroscopy) correlations of the protons in the six-membered ring. The magnitude of the 1 J CH value (171 Hz) clearly revealed the α-configuration [15], placing the anomeric proton in an equatorial position. The equatorial position of H-2′ was assigned by the ROESY correlation between H-2′ and H-3′. The large 1 H-1 H coupling constant (9.0 Hz) between H-3′ and H-4′ established their anti-relationship. The 1,3-ROESY correlation between H-3′ and H-5′ established the axial position of H-5′ ( Figure 3). The analysis of the relative configuration of the sugar showed that it was a rhamnose moiety, which exists solely in the L-form in nature. We tried to determine the absolute configuration of C-8 in amycofuran (1) comparing the experimental CD (Circular Dichroism) spectrum with the calculated ECD (Electronic Circular Dichroism) spectra. Initially, because of the glycosidic bond between rhamnose and benzofuran in 1, which can cause various changes in conformation during energy-minimized structure calculation, we carefully analyzed ROESY correlations between the sugar and aglycone to obtain a most probable conformation (see Supplementary Information). After the calculation of the energy-minimized structure from the conformation based on the ROESY correlation, we calculated ECD spectra [16,17]. The ECD spectra based on the conformation were calculated for the 8S and 8R configurations. The ECD spectrum for 8R turned out to be consistent with the observed CD spectrum, proposing the 8R configuration in amycofuran ( Figure 4). In literature, a fungal metabolite asperfuran, which is aglycone of 1, also displayed a very similar CD spectrum to that of 1, also supporting the 8R configuration (see Supplementary Information) [18]. Amycocyclopiazonic acid (2) was purified as a white powder. Its molecular formula was determined to be C 18 H 20 N 2 O 2 based on high-resolution mass spectrometry data as well as 1 H and 13 C NMR spectroscopic data ( Table 2). The UV spectrum of 2 displayed characteristic indole UV absorption maxima at 222 and 280 nm. The 1 H NMR spectrum showed one downfield singlet proton ( H 11.79) attached to a heteroatom and four aromatic resonances between 7.46 and 7.08 ppm. Two protons bound to heteroatom-bearing carbons were observed at  H 4.83 and 4.18. In addition, the 1 H NMR spectrum displayed six aliphatic protons between 3.76 and 2.47 ppm and two methyl singlets in the upfield region ( H 1.74 and 1.65). The 13 C and HSQC NMR spectra exhibited one carbonyl carbon ( C 170.4); eight double-bond signals from 134.5 to 109.0 ppm, including four methines and four quaternary carbons; two heteroatom-bearing sp 3 methine carbons at  H 73.9 and 73.6; one quaternary carbon at  C 60.6; two aliphatic methines at  C 53.2 and 39.7; two aliphatic methylenes at  C 46.8 and 27.0; and two methyl groups at 26.4 and 22.4 ppm. An analysis of the COSY and HMBC correlations established the expected indole moiety. Next, the COSY correlations and 1 H-1 H coupling constants (7.5-8. (Figure 2). The C-12-C-11 connectivity was determined from the COSY correlations between H 2 -12 ( H 3.13 and 3.03) and H-11 ( H 2.47). C-11 was then connected to C-4 by the homonuclear coupling between H-11 and H-4 ( H 3.76). The spin system was further extended to C-5, C-6, and C-7 by an array of COSY correlations involving H-5, H-6, and H 2 -7. The HMBC correlation from H 2 -7 to C-8 ( C 170.4) extended the connection to the carbonyl carbon, which was verified as an amide carbon based on the IR absorption at 1671 cm −1 . HMBC cross peaks from the methyl groups ( H 1.65 and 1.74) to C-10, C-11, C-19, and C-20 indicated a gem-dimethyl moiety connected to C-11. The carbon chemical shift of C-10 ( C 60.6) indicated that this carbon bears a nitrogen atom, placing the nitrogen atom of the amide functional group next to C-10. Finally, the connectivity from C-5 to the amide nitrogen atom was deduced by the carbon chemical shift of C-5 ( C 73.6) and the degree of unsaturation. Therefore, the planar structure of 2 was elucidated, and it was determined to be a new member of the indole alkaloid cyclopiazonic acid class.
The relative stereochemistry of 2 was readily assigned by ROESY NMR spectroscopic analysis. Strong ROESY correlations among H-4, H-5, and H-6 revealed that these protons are on the same side. The ROESY correlation between H-4 and H-11 established their syn-relationship, thus determining the entire relative configuration of 2. The absolute configuration of amycocyclopiazonic acid was assigned by the modified Mosher method [19]. The esterification of 2 with Rand S-MTPA-Cl (α-methoxy-α-(trifluoromethyl) phenylacetyl chloride) yielded the Sand R-MTPA esters of 2 (4 and 5, respectively). The analysis of the 1 H NMR  S-R values revealed a clearly consistent sign distribution, thus confirming the absolute configuration of 2 as 4R, 5R, 6R, and 11R ( Figure 5).  .70 and 1.67). The 13 C and HSQC NMR spectra enabled the identification of one quaternary carbonyl carbon ( C 176.8); ten sp 2 carbons, including five methines and five quaternary carbons between 138.7 and 110.6; two oxygen-bearing methines ( C 74.2 and 71.4); one nitrogen-bound methine ( C 57.1); two aliphatic methylenes ( C 32.7 and 27.9); and two methyl groups ( C 25.8 and 18.0).
Three partial structures were identified on the basis of the analysis of the COSY and HMBC NMR spectra. In accordance with the UV spectrum, an indole moiety was readily assigned in a manner analogous to that used for amycocyclopiazonic acid (2). A dihydroxy γ-lactam ring was indicated by the COSY correlation from H-6 ( H 4.73) to H-5 ( H 4.26) and H-7 ( H 4.76) as well as the long-range heteronuclear coupling from H-5 and H-7 to the amide carbon C-8 ( C 176. 8). An additional COSY correlation between H-5 and H 2 -4 ( H 3.93 and 3.78) connected the lactam ring to the C-4 methylene. The HMBC correlation from the methyl group protons H 3 -19 ( H 1.67) and H 3 -20 ( H 1.70) to C-10 ( C 131.9) and C-11 ( C 125.1) constructed a dimethyl group bonded to an alkene carbon. The double bond was connected to an aliphatic methylene C-12 ( C 32.7) by the 1 H-1 H coupling between H-11 ( H 5.56) and H 2 -12 ( H 4.02), allowing the assignment of an isoprene unit. These three partial structures were assembled by heteronuclear correlations. The HMBC coupling from H 2 -4 to C-3 ( C 112.6) connected the dihydroxy γ-lactam ring to the indole ring. Furthermore, the two-bond heteronuclear correlation from H 2 -12 to C-13 placed the isoprene unit next to C-13 ( C 134.8), finalizing the planar structure of amycolactam (3) and indicating that it is a new indole alkaloid bearing an isoprene unit and a dihydroxy γ-lactam ( Figure 2). The relative configuration of the dihydroxy γ-lactam ring was firstly deduced from the ROESY correlations and 1 H-1 H coupling constants. A clear correlation between H-5 and H-7 led to the well supported assignment of a syn-relationship between these two protons. However, the chemical shifts of H-6 ( H 4.73) and H-7 ( H 4.76) were too close to allow the definitive assignment of the relative configuration of C-6 from the ROESY correlation. Thus, we analyzed the 1 H-1 H coupling constants in the dihydroxy γ-lactam ring by comparing them with literature values (Figure 6). The coupling constants 3 J H5H6 and 3 J H6H7 in 3 were 5.0 and 3.5 Hz, respectively. These values are consistent with the coupling constants of a dihydroxy γ-lactam ring with the S*, R*, and R* configurations, whereas an alternative isomer with the S*, S*, and R* configurations showed significantly different coupling constants [20][21][22]. To determine the absolute configuration, we first attempted to use the modified Mosher method, but amycolactam (3) decomposed during MTPA esterification, even under mild conditions. Therefore, ECD calculation was utilized to determine the absolute configuration of 3. When we performed the ECD calculation for 3, we found that the existence of two or more conformations. Then, we optimized the conformers of 3 using the basis set def-SV(P) for all atoms and the functional B3LYP/DFT level and considered the conformers when calculating the ECD spectra with TDDFT using the basis set def2-TZVPP for all atoms and the functional B3LYP/DFT level. The calculated ECD spectra of (5S, 6R, 7R)-amycolactam (3) displayed negative Cotton effects at 242 nm, consistent with the experimental CD spectrum (see the Supplementary Information).
The structure of amycofuran (1), which is a benzofuran glycoside, is interesting. To the best of our knowledge, no benzofuran substituted together with a diene moiety and a rhamnose sugar has been previously reported. A comprehensive literature search indicated that benzofurans are very rare as bacterial secondary metabolites, although a few examples, such as actiketal [23] and isoaurostatin [24], have been reported from actinomycetes whereas benzofurans are relatively common in fungi [18,25]. Amycocylopiazonic acid (2) is a new member of the cyclopiazonic acid class, which was previously reported to be produced by fungi belonging to the genus Penicillium [26]. Interestingly, this class has not been discovered from a bacterium. Therefore, amycocyclopiazonic acid (2) is the first bacterially produced member of the cyclopiazonic acid class. Based on the carbon backbone structure of amycolactam (3), this new alkaloid may be considered as an acyclic version of amycocyclopiazonic acid (2) generated during cyclopiazonic acid biosynthesis [27]. The biological activities of 1-3 were evaluated in antibacterial and antifungal assays against various pathogenic microbes, but the compounds did not exhibit significant inhibitory activities. The cytotoxicities of 1-3 were tested against human carcinoma cell lines, including A549 (lung cancer), HCT116 (colon cancer), SNU638 (gastric cancer), K562 (leukemia), SK-HEP1 (liver cancer), and MDA-MB231 (breast cancer). Amycolactam (3) significantly inhibited the proliferation of SNU638 and HCT116, with IC 50 values of 0.8 and 2.0 M, respectively. The cytotoxic effects of 3 against A546, K562, and SK-HEP1 were moderate (IC 50 = 13.7, 9.6, and 8.3 M, respectively), whereas virtually no cytotoxicity was observed against MDA-MB231. However, amycofuran (1) and amycocyclopiazonic acid (2) did not exhibit significant cytotoxicity against the cancer cells tested.

General Experimental Procedures
Optical rotations were obtained in a 1 cm cell with a Jasco P-1020 polarimeter. UV spectra were measured with a Perkin Elmer Lambda 35 UV/VIS spectrometer. IR spectra were recorded with a Thermo N1COLET iS10 spectrometer. A Bruker Avance 600 MHz spectrometer at NCIRF (National Center for Inter-University Research Facilities, Seoul National University, Seoul, Korea) was used to obtain 1 H, 13 C, and 2D NMR spectra. Electrospray ionization (ESI) low-resolution LC/MS data were collected on an Agilent Technologies 6130 Quadrupole mass spectrometer attached to an Agilent Technologies 1200 series HPLC using a reversed-phase C 18 column (Phenomenex Luna, 100 × 4.6 mm, Torrance, CA, USA). High-resolution electrospray ionization (HR-ESI) mass spectra were acquired with a Thermo Finnigan LCQ high-resolution mass spectrometer at NICEM (National Instrumentation Center for Environmental Management, Seoul National University, Seoul, Korea). Circular Dichroism was measured with an Applied Photophysics Chirascan plus (Surrey, UK) with a 0.2 cm cell at NICEM.

Isolation of Bacteria, Cultivation, and Extraction
A sponge sample was gathered from Micronesia. One gram of unidentified sponge sample was extracted with 3 mL of sterilized artificial seawater at a 1:3 dilution ratio and shaken at 180 rpm for 1 h. A total of 150 L of the resulting solution was spread on Gause1 agar (20 g soluble starch, 1 g KNO 3 , 0.5 g K 2 HPO 4 , 0.01 g FeSO 4 · 7H 2 O), M2 agar (6 mL glycerol, 1 g arginine, 1 g KH 2 PO 4 , 0.5 g MgSO 4 · 7H 2 O, 16.0 g agar), SC agar (10.0 g starch, 0.3 g casein, 2.0 g KNO 3 , 2.0 g K 2 HPO 4 , 0.05 g MgSO 4 · 7H 2 O, 0.02 g CaCO 3 , 0.01 g FeSO 4 · 7H 2 O), IM6 agar (0.5 g glycerol, 0.5 g starch, 0.5 g sodium propionate, 0.1 g KNO 3 , 0.1 g asparagine, 0.3 g casein, 0.5 g K 2 HPO 4 , 1 mg FeSO 4 · 7H 2 O, 16.0 g agar, 1 mg vitamin B), and K agar (25 g soluble starch, 15 g soy peptone, 2 g dry yeast, 4 g CaCO 3 ). The strain Cra33g was isolated on Gause1 agar. An analysis of the 16S rDNA sequence ( Figure S23 in the Supplementary Information) indicated that Cra33g is most similar to Amycolatopsis saalfeldensis. The strain Cra33g was cultivated in 60 mL S medium (25 g soluble starch, 15 g soy peptone, 2 g dry yeast, 4 g calcium carbonate in 1 L artificial seawater) in a 150 mL Erlenmeyer flask. After cultivation for 70 h on a rotary shaker at 180 rpm at 27 °C, a 5 mL aliquot of the culture was transferred into 250 mL Diaion LH-20 (1 g/L) with GSS medium (10 g soluble starch, 20 g glucose, 25 g soy peptone, 1 g beef extract, 4 g yeast extract, 2 g NaCl, 0.25 g KH 2 PO 4 , and 2 g CaCO 3 in 1 L artificial seawater) in 500 mL Erlenmeyer flasks (20 ea × 250 mL; total volume 5 L). After 10 days of cultivation, 18 L of ethyl acetate was added to the whole culture (5 L) and shaken for extraction. Anhydrous sodium sulfate was added to the separated organic layer, which was then filtered and dried. In total, the ethyl acetate extract yielded 4.5 g (a rotary evaporator was used to concentrate the extracts in vacuo). This procedure was performed 10 times (50 L culture; total amount of extract: 45 g) to acquire quantities of 1-3 sufficient for structure elucidation and bioassays.

MTPA Esterification of Amycocyclopiazonic Acid (2)
Two 40 mL vials containing 2 (1 mg sample in each vial) were completely dried under high vacuum for 10 h. Freshly distilled anhydrous pyridine (1 mL) and catalytic amounts of crystalline dimethylaminopyridine (DMAP) were added to each reaction vial in that order under argon gas. Then, Rand S-α-methoxy trifluoromethyl-phenylacetic acid (MTPA) chloride (20 μL of R-MTPA-Cl and 60 μL of S-MTPA-Cl) were separately added. Whereas the reaction with R-MTPA chloride was completed at room temperature after 90 min, the reaction with S-MTPA chloride required 2 h at 50 °C. The reactions were quenched by the addition of 50 μL of MeOH. The products were purified using reversed-phase HPLC (Kromasil C 18 (2)

ECD Computational Calculation
The theoretical calculations of compound 1, the aglycone of compound 1, and 3 were performed using Turbomole 6.5. The optimized conformation geometries and energy levels are provided in the Supplementary Information (Tables S1-S3). The theoretical calculation of ECD was performed using TDDFT using the basis set def2-TZVPP for all atoms and the functional B3LYP/DFT level. The ECD spectra are obtained by Gaussian functions for each transition. (1) where σ represents the width of the band at 1/e height and ΔE i and R i are the excitation energies and rotational strengths for transition i, respectively. σ = 0.10 eV and R vel°city were used in this work.

Evaluation of Anti-Proliferative Activity
Cell proliferation was measured using the sulforhodamine B (SRB) assay. Briefly, six human cancer cell lines (A549, HCT116, SNU638, K562, SK-HEP1, and MDA-MB231) (3 × 10 5 cells/mL) were seeded in 96-well plates with various concentrations of 1-3 and incubated at 37 °C in a humidified atmosphere with 5% CO 2 . After 72 h of treatment with amycofuran (1), amycocyclopiazonic acid (2), and amycolactam (3), the cells were fixed with a 10% TCA solution for 1 h, and cellular proteins were stained with 0.4% SRB in a 1% acetic acid solution. The stained cells were dissolved in 10 mM Tris buffer (pH 10.0). The effect of 1-3 on cell proliferation was calculated as the percentage relative to a solvent-treated control, and the IC 50 values were determined using nonlinear regression analysis (percent survival versus concentration).