- freely available
Mar. Drugs 2014, 12(12), 5719-5763; doi:10.3390/md12125719
Abstract: The emergence of marine toxins in water and seafood may have a considerable impact on public health. Although the tendency in Europe is to consolidate, when possible, official reference methods based on instrumental analysis, the development of alternative or complementary methods providing functional or toxicological information may provide advantages in terms of risk identification, but also low cost, simplicity, ease of use and high-throughput analysis. This article gives an overview of the immunoassays, cell-based assays, receptor-binding assays and biosensors that have been developed for the screening and quantification of emerging marine toxins: palytoxins, ciguatoxins, cyclic imines and tetrodotoxins. Their advantages and limitations are discussed, as well as their possible integration in research and monitoring programs.
Marine toxins constitute a heterogeneous group of complex chemical compounds, produced as secondary metabolites by bacteria and microalgae (e.g., dinoflagellates, diatoms, cyanophyceae). Although not fully understood, specific environmental conditions and biological cycles can modulate microalgal population dynamics and may cause the appearance of harmful algal blooms (HABs) and their toxins, which may affect the ecosystems. Among other organisms, fish or shellfish can accumulate marine toxins produced by microalgae, thus entering in the food webs and occasionally posing a threat to human consumers. Other routes of human exposure to marine toxins, apart from oral consumption of contaminated seafood, are respiration and skin contact.
The concept “emerging marine toxins” is quite subjective. It has been used by regulatory organizations such as the European Commission and the European Food Safety Authority (EFSA) [1,2,3,4,5] to focus on recently discovered marine toxins (e.g., some cyclic imines like pinnatoxins), but also those known marine toxins that appear in waters and seafood where they were previously absent (e.g., ciguatoxins, recently appearing in the eastern Atlantic, Macaronesia). “Emerging marine toxins” has also been applied to non-regulated known marine toxins, which are considered as a possible matter of concern but for which additional toxicological evidence is needed before establishing further regulations (e.g., palytoxins and brevetoxins).
The little or no toxicological information available, the structural complexity of marine toxins, and the scarcity of purified standards have hindered the development of methodologies for their detection and their subsequent regulation by the establishment of maximum permitted levels (MPLs). Mouse bioassays (MBAs) have been for years important tools to manage seafood safety and prevent risk situations for consumers. For example, the application of the MBA for the management of Paralytic Shellfish Poisoning (PSP) toxins may be, in some situations, the safest approach since other methods may not assure a good comprehension of the risk due to the complexity of this group of toxins. In addition, MBAs, among other bioassays, may be of interest to understand the toxic potential and mechanism of action of new marine toxins and may also contribute to unexplained toxicity of seafood. Nevertheless, European authorities, having specific regulations that forbid the use of laboratory animals when equivalent alternative methods exist, encourage and facilitate the development of alternative methods . Moreover, as the MBAs are non-specific methods, they do not allow the quantification of individual toxins in seafood.
As for other contaminants, instrumental analysis methods have been applied to detect and quantify some marine toxins in order to fulfil regulations. For example, Amnesic Shellfish Poisoning (ASP) toxins present in seafood are quantified using high-performance liquid chromatography (HPLC-UV) . In the EU, a liquid chromatography-coupled to tandem mass spectrometry (LC-MS/MS) method is currently the reference method for the quantification of lipophilic marine toxins , replacing completely the MBA by 2015. For PSP toxins, although the reference method in the EU is a MBA, the Lawrence HPLC method using pre-column derivatisation and fluorescence detection is also an official control method , but depending on the PSP toxins profile this method is not always applicable and, therefore, the correct quantification of analogues that coelute is not possible. Other HPLC methods for PSP toxins using post-column derivatisation are also available [9,10,11]. Instrumental analysis approaches have also been contemplated for emerging marine toxins. For example, cyclic imines such as gymnodimines, pinnatoxins and spirolides, can be identified and quantified by LC-MS/MS methods [12,13,14,15,16]. Ciguatoxins can also be identified by LC-MS/MS, but the application of the method in routine is difficult due to the lack of certified material, the structural complexity of the toxins group, and the low sensitivity of the current instrumentation, since these toxins are extremely powerful and may be hazardous at concentrations in fish that are difficult to detect [17,18].
Although the instrumental analysis approach is certainly a good strategy to evaluate emerging marine toxins in food, alternative methods that can provide a higher sensitivity or that may have other advantages such as shorter analysis times or lower cost, may be used as screening or quantification tools to facilitate the evaluation of multiple samples in due time and at regulatory levels. Nevertheless, for some emerging marine toxins, the combination or coupling of instrumental analysis techniques with alternative methods could be the most appropriate approach to quantify them in seafood and evaluate their risk.
In this review, we present a detailed overview of alternative or complementary methods to the MBAs and instrumental analysis techniques for the detection of some emerging marine toxins. The advantages and drawbacks of biosensors, biochemical assays and cell-based assays for the detection of palytoxin (PLTX), ciguatoxin (CTX), tetrodotoxin (TTX) and cyclic imines (CIs) are discussed.
2. Biosensors, Biochemical Assays and Cell-Based Assays
Biosensors, biochemical assays and cell-based assays are promising tools to overcome MBA drawbacks and to complement instrumental analysis techniques, because of their selectivity, sensitivity, ease-of-use and low cost. In this review, the term biochemical assay includes both immunoassays and receptor-binding assays (RBAs). For each toxin group, immunoassays are first described, followed by cell-based assays (CBAs) and finally RBAs (the latter sometimes being based on the same mechanism of action than CBAs but using receptors instead of whole cells). Since biosensors can be based on any of the assays, they are described at the end of each corresponding section.
Immunoassays are biochemical assays based on the immunological affinity between an antibody (Ab) and its antigen. They have been successfully used in both the screening and precise quantification of some marine toxins, because of their high selectivity and sensitivity. It is necessary to keep in mind that immunoassays are based on a structural recognition, thus they do not provide toxicological information. Moreover, Abs may have the ability to detect different toxin analogues or derivatives, if they share a structurally similar fragment, although may be to a different extent. This cross-reactivity may be advantageous or not, depending on whether the purpose is to detect the whole family of toxins (not all of them necessarily having the same toxicological potency) or just a specific one. The most common immunoassay format is the enzyme-linked immunosorbent assay (ELISA), which relies on the use of specific Abs against the target analyte, and enzymes as labels. ELISAs can be direct or indirect. The indirect format involves the use of a labelled secondary Ab against the primary Ab, and the direct one implies the labelling of the primary Ab. While the indirect assays require more steps and thus longer analysis times, the direct approach provides higher sensitivities and shorter analysis times, but the primary Ab labelling may not be straightforward. ELISAs can also be competitive or sandwich. In competitive assays the free and immobilised toxins compete for a capture Ab and, if required, a labelled secondary Ab is added to the system. In sandwich assays the target analyte is sandwiched between two Abs: a capture Ab, which is usually immobilised and recognizes the analyte of interest, and a detector Ab, which also recognizes the antigen but not at the same antigenic site. Again, labelled secondary Abs that recognize the detector Ab may be needed. Consequently, in the development of sandwich immunoassays, only large molecules with different antigenic epitopes can be targeted. Immunostrip or immunostick tests are specific types of immunoassay, which commonly use paper pads as immobilisation supports, characterized by their rapidity, portability and ease of use. Compared to other more sophisticated techniques, they are less sensitive but allow the screening of toxins in situ in only a few minutes by simple visual reading.
Cell-based assays (CBAs) are assays based on the toxicological effect of toxins on cells. Marine toxins usually produce a change in the physiology, the morphology or the viability of cells, which can be measured and quantified. Most CBAs require the presence of agonists or antagonist, e.g., the drugs veratridine and ouabain, in order to counteract or emphasize the action of those toxins. Veratridine is a well-known activator of the voltage-gated sodium channels (VGSCs), which binds to these channels and blocks them in an open position. Ouabain binds to the Na+/K+-ATPase pump and blocks it in a closed position, thus impeding the flux of sodium from the interior of the cells. Toxins acting on these channels and pumps, in the presence or absence of ouabain and veratridine at appropriate concentrations, will involve a specific response on cells. In this case, different toxins or analogues sharing the same mechanism of action may act on the cells to a different extent and therefore may have different toxic potency. It is necessary to differentiate those assays implementing primary cultures from those performed with established immortal cell lines. Primary cell cultures are obtained from tissues some hours or days prior to the execution of the assay. They present the advantage of reflecting, to a larger extent, the properties that the cells have in the organism, for example in regard to the presence and amount of membrane receptors where the toxins act. In that sense, these models could be more appropriate to study some mechanisms of action of the toxins and could be more sensitive than immortal cell lines. However, the use of primary cells may be more complex than immortal cell lines, as they may involve the use of laboratory animals. In addition, primary cell cultures may present a higher variability than immortal cell lines regarding their physiology and functional properties, which are related to the organism source and the cell isolation process. Despite the advantages of primary cultures in terms of mechanism of action and high sensitivities, their use in CBAs for the determination of emerging toxins has not been extensively exploited.
The hemolytic test is a specific CBA based on the lysis of red blood cells (RBCs) in the presence of compounds that alter the osmotic equilibrium. Rather than a primary culture, RBCs should be considered as tissue samples since they lack a nucleus and are terminally differentiated. RBCs contain hemoglobin in their cytoplasm. When lysis occurs after exposure of RBCs to the toxins, hemoglobin is released and its absorbance can be measured. The hemolytic test can be applied to the detection of specific marine toxins that have the ability to bind to specific ion channels located in the RBCs membranes. Like other CBAs, in order to gain specificity an antagonist is needed. Hemolytic assays may be defined taking into account the toxin mechanism of action and the RBCs origin, since variability in the response may exist depending on the source of the cells (species, population, individual). As for any toxicological assay, the time of exposure, among other parameters, should be clearly defined.
Receptor-binding assays (RBAs) are assays based on the ability of cellular receptors to bind to a specific ligand. In these assays, the competition between a labelled toxin and the toxin present in the sample for the receptor is usually carried out. Originally, ligands were labelled with radioactive moieties, but in the later years, fluorescence and chemiluminescence labels have been exploited, avoiding hazardous waste and attaining also very low limits of detection. Like in immunoassays, cross-reactivity from structurally-related toxins may exist. Since RBAs use biomolecules that have been isolated from cells, these may help to better understand the mechanism of action of toxins.
Biosensors are bioanalytical devices consisting of a biorecognition element, which specifically recognizes the analyte of interest, in intimate contact with a transducer, which converts the biorecognition event into a measurable signal. Their specificity, sensitivity, simplicity and ease of use, together with the possibility to be developed for multiplex detection and to be miniaturised for portability purposes, make the development of biosensors for marine toxins highly desirable. Most biosensors for emerging marine toxins are surface plasmon resonance (SPR) immunosensors, an optical technique that allows the detection of the toxin of interest in real time and without the need of labels. Fluorescence, fluorescence polarisation (FP), electrochemiluminescence (ECL) and electrochemical detection have also been exploited.
Figure 1 shows the chemical structure of some representative toxins of each toxin group, which are described in detail in the following sections.
Table 1 summarizes the emerging marine toxins included in this review, their producer organisms and vectors, the associated syndromes and effects in humans, and the geographic locations where they have been detected in food.
|Toxin||Principal Derivatives||Producer Organism||Vectors||Syndromes and Effects in Humans||Endemic Areas|
|PLTX||Palytoxins||Palythoa||Fish, Molluscs, Crustaceans, Echinoderms||Clupeotoxism |
Skeletal muscle injury
|Australia, New Zealand, French Polynesia, Cook Islands, Japan, Indonesia, Micronesia, Malaysia, Vietnam, Philippines, Singapore, Hawaii, Caribbean, Mexico, Brazil, Madagascar, Reunion Island, Mediterranean coastline of Europe|
|Putative PLTX |
|Ostreopsis cf. ovata|
|Gambierdiscus sp.||Fish||Gastrointestinal effects |
|French Polynesia, Cook Islands, Hawaii, Japan, Mexico, Tokelau, North Marianna, Tuvalu, Marshall Islands, Fiji, New Caledonia, Tonga, Vanuatu, Samoa, Kiribati, Australia, Micronesia Sri Lanka, Hong Kong, Vietnam, Indonesia, Madagascar, Reunion Island, Maldive Islands, Gulf of Mexico, Guadeloupe, Florida, Virgin Islands, Puerto Rico, Brazil, Canary Islands, Madeira|
|CIs||Gymnodimines||Gymnondinium selliformis||Shellfish||Not reported||Canada, New Zealand, Japan, Denmark, Ireland, Norway, Spain, Italy, Tunisia|
|TTX||Tetrodotoxins||Vibrio sp. |
|Pufferfish, Starfish, Blue-ring Octopus, Xanthid Crabs, Gastropods, Flatworm, Frogs, Goby, Newt Taricha||Ichthyosarcotoxism |
|Japan, China, Taiwan, Madagascar, Australia, New Zealand, Korea, India, New Hampshire, New York, Pennsylvania, Viriginia, Chicago, California, Hawai, Bangladesh, Thailand, Norway, Meditaerranian region (Israel, Egypt and Greece), Spain (fish caught in Portugal)|
Palytoxins (PLTXs) are one of the most poisonous non-protein marine toxins. They have an acute toxicity in mice, specifically toxic by intravenous injection (lethal dose (LD50) ranging from 0.15 to 0.73 µg/kg) . Despite their high toxicity in animals, few cases of human poisoning have been reported. PLTX was originally isolated from soft coral Palythoa sp. , which belongs to the family of Zoanthidae. This zoanthid was subsequently identified as Palythoa toxica . Nearly a decade later, the structure of PLTX was described by two groups independently [22,23]. Later on, PLTX analogues named homopalytoxin, bishomopalytoxin, neopalytoxin, deoxypalytoxin and 42-hydroxypalytoxin  were isolated from Palythoa tuberculosa . PLTX-group toxins are large and complex molecules with a polyhydroxylated and partially unsaturated aliphatic backbone, which contains 64 chiral centers and presents both lipophilic and hydrophilic regions . The molecular weights of PLTX and its analogues differ from 2659 to 2680 Da.
Furthermore, other PLTX analogues were described in different species belonging to the benthic dinoflagellate Ostreopsis in tropical areas: ostreotoxins 1 and 3 in O. Lenticularis , ostreocin-D in O. Siamensis [28,29], and mascarenotoxins  in O. mascarenensis. In recent decades, several studies have associated O. cf. ovata seasonal blooms in the Western Mediterranean with respiratory illness in humans  and adverse effects in marine organisms  (e.g., sea urchins lost their spines and died). PLTX-like compounds have been identified in the Mediterranean strains, such as putative palytoxin (pPLTX) , ovatoxins-a , -b, -c, -d  and -f . PLTXs congeners have also been identified worldwide in echinoderms, molluscs (shellfish and cephalopods), crustacean and fish [36,37].
Great efforts have been made to clearly understand the mechanism of action of PLTX. At a cellular level, PLTX blocks the Na+/K+-ATPase pump . The activity of this transmembrane protein is essential for the maintenance of the cell homeostasis, which actively transports 3 Na+ out of the cell and 2 K+ in, by hydrolysing ATP under normal conditions. PLTX binds to the extracellular part of the Na+/K+-ATPase, inhibiting the active ion exchange by converting the pump into a permanently open ion channel, eventually causing cell lysis . Moreover, an increase in the cytosolic Ca2+ caused by the action of a Na+-Ca2+ exchange pump is observed [40,41], also associated with cell death, apart from other secondary effects including cytoskeleton, cardiac and muscles contraction.
The potential routes of human exposure to PLTXs are seafood consumption and dermal and inhalation exposure to aerosols from Ostreopsis sp. blooms . The most commonly reported symptoms of PLTX poisoning include: rhabdomyolisis, skeletal muscle injury, decrease in myocytes content in blood plasma, bitter/metallic taste, abdominal cramps, nausea, vomiting, diarrhea, paresthesia, bradycardia, renal failure, cyanosis and respiratory distress, and even death in the worst cases . Additionally, clupeotoxism is a rarely occurring highly fatal form of human intoxication in tropical areas due to ingestion of clupeoid fish (some species of sardines and herrings), which was described for the first time in 1994 and attributed to the presence of pPLTX .
The MPLs of PLTX-group toxins in shellfish are not established. Nevertheless, in 2005 the European Union Reference Laboratory for Marine Biotoxins (EU-RLMB) set a provisional limit of 250 µg/kg of PLTX in shellfish . Later on, EFSA suggested to decrease the limit to 30 µg/kg of the sum of PLTX and ostreocine-D in meat . However, these are only recommendations and not regulations. Nowadays, there is no recognized official method for the determination of PLTX-group toxins.
2.1.1. Immunoassays and Biosensors for Palytoxins
Contrary to TTX and CIs but similarly to CTX, the large size of PLTX is an advantage for Ab production via animal immunisation, thereby simplifying the development of immunoassays. In that sense, highly specific Abs have been raised by the immunisation of mice against PLTX conjugated to carrier proteins such as keyhole limpet hemocyanin (KLH)  and bovine serum albumin (BSA) [46,47,48], through the amine group of the toxin. The specificity of the produced Abs has been assessed by the development of immunoassays using both direct and indirect strategies. Two indirect competitive ELISAs have been reported so far [45,47]. While Bignami et al.  used the classical PLTX-BSA conjugate as a coating agent, Frolova et al.  took advantage of its large size and immobilised bare PLTX directly on the microtiter plate surface. The IC50 values obtained were 6.2 and 20 ng/mL, respectively. The applicability of these methods has been demonstrated by the analysis of naturally-contaminated samples of the coral P. Tuberculosa  and bacterial (Aeromonas sp. and Vibrio sp.) extracts from sea organisms including sponges, mussels and echinoderms . Using the same Abs, Bignami et al.  developed two direct competitive immunoassays with the aim of reducing time and costs, using alkaline phosphatase (ALP)-labelled monoclonal antibodies (MAbs) or PLTX. Both strategies showed good sensitivity towards PLTX in spiked shellfish samples (IC50 of 3.5 and 10.1 ng/mL for MAb-ALP and PLTX-ALP, respectively), which allowed to decrease the analysis time from 4 to 2 h approximately.
Sandwich immunoassays using capture and detection Abs have also been developed for the detection of PLTX. In this direction, a direct  and two indirect [45,49] approaches have been reported. The direct strategy involved an ALP-labelled polyclonal antibody (PAb) and provided an IC50 of 4.8 ng/mL of PLTX. Interestingly, the same author  developed one of the two indirect sandwich assays, which resulted in longer analysis times but, unexpectedly, higher sensitivity (IC50 of 0.6 ng/mL). In the development of the second indirect assay , the affinity of the antibody towards PLTX and the analogue 42-OH-PLTX was first characterized by SPR. The affinity of the Ab seemed to depend on the method used to immobilise PLTX on the sensor chip. The ELISA assay provided an IC50 of 7.6 ng/mL and a limit of detection (LOD) of 1.1 ng/mL. Whereas Bignami’s assay  was applied to the analysis of the coral P. tuberculosa, Boscolo and co-workers  applied it to PLTX-spiked mussels, microalgae and seawater samples, matrices that implied only a slight shifting in the sensitivity of the assay.
Beyond the traditional method for MAb production, Garet and co-workers  reported the first production of highly specific recombinant Abs against PLTX using recombinant hybridoma technology. Compared to the traditional methodology, this technology presents advantages in terms of production of affinity Ab fragments without the need for animal immunisation nor antigen coupling to a carrier protein, thus reducing time and costs. In this work, the selected phage-antibody clones against PLTX were first characterized, and the most specific one was used to develop the indirect competition assay. The recombinant Ab was able to recognize free and immobilised PLTX with the best specificity ever reported up to date, even using an indirect strategy (LOD = 0.5 pg/mL). The applicability of the assay was demonstrated by the analysis of spiked samples of mussels and clams, without sample purification steps, which showed good recoveries and no or little matrix effects.
Regarding biosensors for PLTX detection, a sandwich immunoassay combined with ECL detection has been developed . The immunosensor consisted of doubly amino-functionalised multi-walled carbon nanotubes (MWCNTs): CNT sidewalls were linked to MAb anti-PLTX (capture Ab) and CNT tips to the surface of an optical transparent electrode covered by an electrochemical polymer layer. The electroluminescent detection was achieved by labelling the anti-PLTX PAb (detecting Ab) with a luminescence ruthenium complex. Firstly, the affinity of the immunoconjugate (CNT-MAb) towards PLTX was evaluated by SPR. Afterwards, the electrochemiluminescent immunoassay was carried out and its applicability was demonstrated using PLTX-spiked mussels and microalgae samples. The biosensor attained an LOD of 0.07 ng/mL and a limit of quantification (LOQ) of 0.24 ng/mL, and it was also suitable for shellfish and microalgae quantification, with LODs of 0.05 and 0.06 ng/mL and LOQs of 0.22 and 0.23 ng/mL, respectively. The CNT layer enhanced the electrochemiluminescent signal, the nanocomponents favoring the electron transfer and increasing the amount of immobilised MAb compared to the system without them.
An SPR biosensor has been developed based on a direct immunoassay . After characterisation of MAb kinetics and optimisation of the experimental parameters, the appropriate performance of the optical immunosensor was demonstrated. This biosensor allowed the determination of PLTX with LODs of 0.52, 2.8 and 1.4 ng/mL in buffer, grouper and clam samples, respectively.
2.1.2. Cell-Based Assays for Palytoxins
CBAs for PLTX detection are based on the ability of PLTX to bind to the Na+/K+-ATPase pump, inhibiting its activity and converting it into a permanently open ion channel. As a consequence, a rapid release of K+ from cells is produced. In order to confer selectivity to the assay, ouabain, a specific inhibitor of the pump, is added to the system. Nevertheless, PLTX does not exactly mimic ouabain action and may not stimulate the same signaling pathways . Actually, the mechanism of action is poorly understood and, at experimental level, some contradictory results have been observed.
The first CBA for PLTX was developed by Bellocci and collaborators  using MCF-7 cells and ouabain as an antagonist, and measuring the reduction of cytosolic lactate dehydrogenase (LDH) after cytolysis. After optimisation of the experimental parameters, the assay provided an EC50 of 530 pM of PLTX. Subsequent works [54,55,56] have used 3-(4,5-dimethylthiadol-2-yl)-2,5-diphenyltetrazolium (MTT) to measure the mitochondrial activity and quantify the cell viability, because it does not involve transferring supernatants and thus increases the reproducibility of the assay.
Comparing CBAs developed with the mouse neuroblastoma (N2a) cell strain, different findings have been reported. Ledreux and co-workers  observed that pre-incubation with ouabain augmented cells viability, but simultaneous addition of PLTX and ouabain increased cell death. Kebrart and collaborators , however, claimed increased cell death in both situations. Pawlowiez and co-workers  also observed amplification of the PLTX effect after pre-incubation with ouabain. Finally, Cañete and Diogène  reported that cell treated with ouabain and veratridine were more sensitive to PLTX than without treatment, both in N2a and neuroblastoma/glioma hybrid (NG108-15) cells. Nonetheless, and despite the differences in sensitivity, all CBAs attained EC50 values in the pM range. In fact, genetic differences between cells or between the subunits of the Na+/K+-ATPase pump, and nuances in the experimental protocol could be responsible for the encountered differences, taking into account the complexity of the mechanism of action of PLTX and its antagonist, not yet fully understood [58,59].
An interesting approach is that reported by Espiña and co-workers , who have developed a dynamic assay using rat hepatocytes (clone 9) and human neuroblastoma BE(2)-M17 cells. In this case, the authors used Alamar Blue, a fluorescent dye, which allowed continuous measurement of cell viability. The preventive action of ouabain on the decrease of viability caused by PLTX was more evident in human N2a cells than in hepatocytes.
Regarding the overall applicability, these CBAs have been useful to detect PLTX-like toxicity in Ostreopsis sp. extracts [53,54,56,60], and even in the marine cyanobacteria Tricodhesmium . The CBA has also been applied to the determination of PLTX in spiked and naturally-contaminated seafood samples [54,56,60].
A consensus regarding PLTX extraction seems to have been achieved in recent studies focused on the O. cf. ovata strain of the Mediterranean Sea. For both microalgae cultures and seafood matrices, the most commonly used solvent is aqueous methanol, varying the proportion between 50 and 100%. For microalgae cultures, various strategies have been conducted with respect to cell collection by centrifugation or filtration, as well as for the time used to disrupt those cells by sonication [61,62,63,64,65,66].
In the early 1980s, Habermann and co-workers [67,68,69] described delayed hemolytic action of PLTX on mammal red blood cells (RBCs). Taking as a basis this effect, and a previous work with the MAb against PLTX , Bignami  developed the first hemolytic assay for PLTX using mouse blood, considered more sensitive to the PLTX effect. As previously mentioned, to verify that hemolysis is specifically due to PLTX, an antagonist should be used. Ouabain was an appropriate antagonist when using human erythrocytes; nevertheless, when using mouse blood, the anti-PLTX MAb seemed to be more effective .
Subsequent assays have derived from Bignami’s test , just with slight modifications in the origin of RBCs and some experimental parameters [43,72,73]. Particularly interesting is the work performed by Riobó and co-workers . Taking into account that the hemolytic effect may not be linear according to the toxin dose, and in an attempt to provide a more reliable assay, they modelled the toxicological dynamics and kinetics of hemolysis by PLTX. The model provided optimal working conditions, indicating the necessity to operate at a moderate temperature (25 °C) and to use ouabain as an antagonist.
Hemolytic assays have been applied to demonstrate the production of PTLX-like compounds by Ostreopsis sp. [30,37,61,62,63,64,65,66,74,75,76], as well as the presence of PLTX analogues in marine organisms such as corals, echinoderms, crustaceans and cephalopods [43,71,73,74,75,77,78,79,80,81,82,83,84] and cyanobacteria . Due to the scarcity of PLTX standards availability, some authors [64,65] have decided to quantify the toxicity in relation to the saponin hemolytic activity, based on Igarashi’s method for other dinoflagellates .
Recently, an innovative method has been reported by Volpe et al.  to measure the hemolytic activity of PLTX. The method is based on the release of LDH from sheep erythrocytes to the medium, caused by the hemolytic activity of PLTX, and the subsequent amperometric detection of the enzyme activity on 8-screen-printed electrode strips. Ouabain was used to ensure the specificity of the assay for PLTX. The optimisation of the electrochemical detection indicated that the best approach was the use of pyruvate and NADH as enzyme substrates and phenazine methosulfate (PMS+). This compound reacts with the remaining NADH to produce PMSH, subsequently reacting with hexacyanoferrate (III) and producing hexacyanoferrate (II), which is oxidised on the electrode. The LOD depended on the hemolysis time, being 0.007 and 0.16 ng/mL for 24 and 4 h, respectively. Since both values are far below the proposed provisional limit, the shortest time was chosen. Compared to the spectrophotometric detection, this method is faster and allows portability.
2.1.3. Receptor-Binding Assays and Biosensors for Palytoxins
Several works have developed assays for PLTX using the Na+/K+-ATPase pump. In a recent study, Na+/K+-ATPase has been labelled with a fluorescent molecule to develop a fluorescence polarisation (FP) assay . The binding between PLTX and the fluorescent pump was detected by measuring the degree of polarisation of the fluorescence light. After protocol optimisation using ouabain as a model, the assay was demonstrated to be useful for PLTX quantification (LOD = 2 nM; LOQ = 10 nM), the FP decreasing proportionally to the toxin concentration. The assay was successfully applied to the analysis of mussel and dinoflagellates cultures extracts, previously cleaned up to minimize matrix interferences.
Optical biosensors, similar to the previous fluorescent assay, have been reported [89,90]. In these works, Na+/K+-ATPase pumps were immobilised on dextran-modified resonant mirrors  or SPR chips , and the PLTX binding was recorded in real time with the corresponding detector. Although the resonant mirror showed a concentration-dependent ouabain binding, no response was observed with PLTX. Since PLTX was neither able to displace bound ouabain, the authors suggested that PLTX and ouabain do not share the same binding site. The lack of response could be justified by steric impediments or the necessity of having the Na+/K+-ATPase pump inserted into the cell membrane for the PLTX binding to occur. When using SPR, the same authors immobilised the Na+/K+-ATPase via thiol coupling instead of amino coupling, with the aim of favoring PLTX binding. In this case, not only ouabain but also PLTX bound to the pump with high affinity (LOD = 3.73 pg; LOQ = 11.20 pg). The biosensor was applied to the analysis of PLTX in Ostreopsis siamensis cultures, demonstrating the viability of the approach.
Table 2 summarizes the biosensors, biochemical assays and cell-based assays that have been developed for the detection of PLTX.
|Immunoassay||Colorimetry||P. tuberculosa and spiked shellfish|||
|Aeromonas sp. and Vibrio sp.|||
|Spiked mussels, microalgae and seawater|||
|Spiked mussels and clams|||
|Immunosensor||ECL||Spiked mussels and microalgae|||
|SPR||Grouper and clams|||
|Spiked and naturally-contaminated seafood||[54,56]|
|Fluorescence||Spiked and naturally-contaminated seafood and Ostreopsis sp.|||
|Hemolytic assay||Colorimetry||Corals (Palythoa sp. and Zoanthus sp.)||[70,77,78]|
|Sponges, soft coral, gorgonians, crustaceans|||
|Mouthed rock shells, sea urchin, mullet, sea-brams|||
|RBA||FP||Mussels and Ostreopsis sp.|||
|Receptor-based biosensor||SPR||Ostreopsis sp.||[89,90]|
Ciguatoxins (CTXs) are potent polyether neurotoxins with 13–14 rings linked by ether groups into a rigid ladder-like structure . They are odourless, tasteless and relatively heat-stable molecules that remain toxic after cooking and freezing, and exposure to mild acidic and basic conditions . CTXs production has been associated to benthic dinoflagellates of the genus Gambierdiscus. The first species described was Gambierdiscus toxicus, originally considered as a producer of maitotoxin (MTX) and gambierols, precursors of CTXs [93,94,95]. Significant variation in toxin production occurs within the genus Gambierdiscus . Later taxonomic studies have led to the organization of the genus into several species, reaching the conclusion that several species within this genus would produce CTXs , being G. polynesiensis a major CTX producer species .
CTXs can enter into the food webs through the consumption of dinoflagellates by herbivorous fish and their subsequent consumption by carnivorous fish . Humans would be intoxicated by consumption of herbivorous and carnivorous fish containing CTXs. CTX precursors accumulate in fish tissue (mainly in viscera, but also in the muscle or other parts ) and may afterwards be metabolized into different CTX forms, which are responsible for human intoxication. Around 30 analogues from the Pacific Ocean (P-CTXs) [101,102,103,104,105,106,107,108], from the Caribbean Sea (C-CTXs)  and from the Indian Ocean (I-CTXs) [18,110] have been identified.
CTXs bind to VGSCs in cell membranes, blocking them in an open state, and cause membrane excitability, release of neurotransmitters, increase of intracellular calcium and blockage of voltage potassium channels [111,112,113,114,115]. This induced depolarisation of nerve cells is believed to cause some of the neurological signs associated with Ciguatera fish poisoning (CFP) . CFP is the foodborne illness caused by consumption of CTX-containing fish, responsible for the highest reported incidence of human poisoning from seafood consumption worldwide [108,117]. This complex syndrome is characterized by a wide variety of symptoms such as gastrointestinal (e.g., vomiting, diarrhea, nausea), neurological (e.g., tingling, itching) and cardiovascular (e.g., hypotension, bradycardia) effects. In severe cases the symptoms may begin in 30 min after ingestion of contaminated fish, while in milder cases they may be delayed for 24 to 48 h. Fatalities may occur due to cardiorespiratory failure. At present, an estimation indicates that between 10,000 and 50,000 people are suffering around the world from this disease annually . CFP is endemically found in Indo-Pacific and Caribbean areas. However, in recent years CTXs are appearing in countries not expected for their latitude, such as waters close to European and African continents, e.g., in the Canary Islands (Spain)  and Madeira (Portugal) . This may be due, for example, to change on the distribution of toxin-producing microalgae to northern latitudes  or to migration of fish containing the toxins .
No regulatory limits exist for CTXs in fish in Europe, but the legislation requires that no fish products containing CTXs are placed on the market . The United States Food and Drug Administration (US FDA) has proposed guidance levels of ≤0.1 μg/kg of C-CTX-1 equivalents and ≤0.01 μg/kg of P-CTX-1 (initially named CTX-1B, both names currently in use) equivalents . In addition, since the unique existing certified standard is for P-CTX-1, toxicity equivalency factors (TEFs) for CTX congeners have been established by acute toxicity in mice (LD50) as follows: P-CTX-1 = 1, P-CTX-2 = 0.3, P-CTX-3 = 0.3, P-CTX-3C = 0.2, 2,3-dihydroxy P-CTX-3C = 0.1, 51-hydroxy P-CTX-3C = 1, P-CTX-4A = 0.1, P-CTX-4B = 0.05, C-CTX-1 = 0.1 and C-CTX-2 = 0.3. These TEFs should be applied to express individual analogues identified with quantitative detection methods as P-CTX-1 equivalents .
2.2.1. Immunoassays and Immunosensors for Ciguatoxins
The production of specific Abs for the development of immunoassays for CTXs has been hampered by the scarcity, toxicity and chemical complexity of CTXs. The first work on this subject  reported the production of sheep PAbs against partially purified CTX-1B, which were subsequently radiolabelled to be used in a radioimmunoassay (RIA). Although the specificity and sensitivity were not fully optimized, this work paved the way towards the development of immunoassays for the detection of CTX in fish. These PAbs were also labelled with horseradish peroxidase (HRP) and used in a competitive ELISA . The application of the assay to the analysis of CFP-related fish provided results similar to those obtained by the previous RIA and also MBA. Two years later, this ELISA was improved in terms of time and simplicity by the development of a colorimetric immunostick test , which was applied to the screening of fish tissue, cooked fish, soup or gravy from CFP outbreaks. The validation of this stick test was carried out with fish caught from CFP areas . The test, marketed as Cigua-Check™, correlated well with the previous immunoassays. Additionally, the authors observed the presence of Gambierdiscus toxicus cells by microscopy in positive fish. Besides the Cigua-Check™, another immunostrip test for CTX detection, named as Ciguatect kit, was developed in 1995 by Park . However, both commercial kits raised controversy regarding their performance, as disagreement with the MBA for the evaluation of CTXs was evidenced, (see comments on [128,129,130]), probably due to the use of the PAb against CTX previously developed by Hokama et al., which showed high cross-reactivity with OA.
In order to overcome this limitation, Hokama and co-workers  produced the first MAbs against CTX, reducing the cross-reactivity towards OA to only 16%. Moreover, they developed the ELISA on oil-based paint-coated beads in order to favor the extraction and enhance the adherence of CTX. These MAbs were also exploited in a stick test format, demonstrating the ability to detect both Pacific and Caribbean congeners (P-CTX-1, P-CTX-2, P-CTX-3 and C-CTX-1) .
Due to the lack of pure CTXs and their high toxicity, synthetic fragments from different parts of CTX have been recently used for the development of Abs and their subsequent use in competitive  and sandwich [134,135,136,137,138,139,140] immunoassays. The rings of CTX are designated from A to M. Abs are usually produced against the right or the left ring fragments, being possible to rationally synthesize them with different lengths (different number of rings). The combination of Abs against different fragments has been an important advantage in the development of sandwich immunoassays, where at least two antigenic sites are needed, one Ab being used as a capture and the other one being labelled with HRP for the direct detection. Moreover, the rational design allows choosing the best strategy depending on the targeted congener. These assays have improved the LODs, being able to detect CTX at nano and even sub-nanomolar levels. Some of these sandwich immunoassays have been applied to the analysis of fish tissues from CFP outbreaks, the results correlating well with those obtained with N2a CBAs [134,135].
2.2.2. Cell-Based Assays for Ciguatoxins
CBAs for CTX detection are based on the capability of CTXs to bind to the VGSCs of cells and block them, in an open state. On excitable cells, this fact results in an influx of sodium ions and cell depolarisation causing spontaneous action potential. If cells are not able to counteract this extra-sodium influx, cells die. In the assay, veratridine (which activates the VGSCs to initiate sodium channel gating) and ouabain (which inhibits the Na+/K+-ATPase pump) are added at selected concentrations maintaining 80% of cells viability. In such conditions, a toxin favoring Na+ influx (like CTXs) will result in cell death. Cell viability is usually quantified by means of the MTT test, which measures the mitochondrial activity. CBAs for CTX have been developed using a range of mammalian tumour cell lines, being the N2a cells the most widely used . Other models, such as NG108-15 cells , have also been proven to be effective for CTX detection. Fairey and Ransdell  developed a N2a CBA to detect VGSC toxins such as CTXs, using luciferase viability as an end-point. Luciferase catalises luciferin oxidation to oxyluciferin using ATP-Mg2+ as a cosubstrate and in the oxidation process the chemical energy is converted into light. In the mentioned work, N2a cells were first transfected with a reporter gene containing the firefly luciferase under the transcriptional regulation of the human c-fos element. In this way, cells were provided with the capability to produce luminescence. In the assay, veratridine was used as an agonist of CTX and, after exposure to toxin, cells were lysed to release the luciferase. The luminiscence intensity was proportional to the ATP produced by viable cells. Nonetheless, the tendency is the application of N2a CBA with MTT due to its suitability, easy handling and resistance.
Brevetoxin (PbTx) and C-CTXs can co-occur in some areas such as the Caribbean and Gulf of Mexico, although they are usually considered responsible for different kinds of intoxication although they share the same mechanism of action. On one hand, blooms of Karenia brevis (PbTx producer) have been associated with massive fish kills as well as marine mammals, turtles and sea birds mortalities ; in humans, PbTx intoxication has been mostly due to shellfish consumption [145,146] although planktivorous fish can also accumulate this toxin . On the other hand, CTX is responsible for CFP after finfish consumption. Latest improvements of the CBAs have been focused on discriminating CTX from PbTx. In this direction, Bottein-Dechraoui and co-workers  combined the CBA with a sodium channel RBA and achieved discrimination, due to the different relative potencies of the toxins and the opposite sensitivity of both assays (C-CTX-1 was always more potent than brevetoxins, but whereas for C-CTX-1 the CBA was more sensitive, for brevetoxins the RBA was more sensitive). The assay was successfully applied to the analysis of barracuda from Florida Keys, CTX being the present toxin. Interestingly, differences in the affinity of PbTx and CTX towards VGSCs from different tissues and species (rat and fish) have been shown by this group .
Manger and co-workers  have recently developed an innovative variant of the N2a CBA using flow cytometry. Unlike the traditional CBA, which uses a horizontal seeding N2a culture, this assay is performed with cells in suspension, and only veratridine is used as an agonist. Moreover, the membrane potential caused by sodium currents resulting from VGSC activation is measured at the end point of the assay using fluorescent voltage-sensitive dyes and a flow cytometer. The response of cells to CTX can be observed even without veratridine as enhancer, although an approximate 1000-fold increase in sensitivity is observed in its presence. This assay reduces the analysis time to minutes and provides more direct mechanistic data.
The applicability of the CBAs to the analysis of different matrices is also under investigation. Toxin extraction and clean-up steps are decisive to obtain a good toxin recovery and avoid matrix interferences. When analyzing flesh fish, the most commonly used protocol for toxin extraction is a modification of that for MBA . All protocols are based in acetone extraction, followed by evaporation and two solvent partitions with methanol:water and hexane, and ethanol:water and diethyl ether, to remove fatty acid and polar compounds [103,151,152,153]. Additional steps with solid-phase extraction (SPE) cartridges can be also performed for further purification  or instead solvent partition for rapidity [155,156]. Some examples of matrices (apart from flesh or viscera fish) where CTX has been detected by CBAs are clams, urchins , mouse dry and fresh blood [157,158], rat plasma, urine, feces and tissues [159,160], and barracuda blood . The application of different extraction and clean-up protocols appropriate for each sample type results in recovery values as high as 90%–96% (in case of mouse and rat blood [158,159,160]) and quantification limits of the order of 0.4 pg P-CTX/mL blood . These examples illustrate the importance of the implementation of extraction and purification steps prior to the assay in order to gain specificity. They also demonstrate that the CBA may be appropriate to quantify CTXs in a wide range of tissues, providing LOQs under the values recommended for P-CTX-1.
When analyzing microalgae, cells are usually broken by sonication and toxins are double extracted with methanol and methanol:water (50:50) . Like in flesh fish, the protocol can include additional purification steps such as solvent partition [76,98,104] and SPE clean-up [54,76]. These procedures have allowed the detection of CTXs in Gambierdiscus species and even cyanobacteria [54,55]. Furthermore, CBA has been successfully applied to detect CTXs dissolved in water by means of absorption resins for lipophilic toxins, without observing any resin matrix interferences . In this work, additionally, fractioning of samples by SPE is proposed as a method to achieve discrimination of toxins.
CTX has also been demonstrated to have hemolytic capacity . However, much work should be performed to obtain optimal LODs in the corresponding assay. Holland and co-workers  proposed a hemolytic assay, which applied to the analysis of different Gambierdiscus sp. extracts. They observed that MTX could be the principal responsible of the hemolytic activity in those microalgae samples. This response may be attributed to the different mechanism of action in Na+/Ca2+ channels among both toxins.
2.2.3. Receptor-Binding Assays for Ciguatoxins
PbTXs have often been used in the development of RBAs for the detection of CTXs because of their similar mode of action (ability to bind to VGSCs) and availability. These RBAs are based on the competition of labelled and free toxin for the sodium channel receptor, different titriated PbTXs being used as tracer and/or competing free toxin for the construction of the dose-response curve. These assays have contributed to the elucidatation of the toxic potencies of CTX derivatives, although different findings have been observed. The first RBA was developed by Lewis and co-workers , who observed differences between C-CTX-1, C-CTX-2 and C-CTX-3 in their competition with tritiated PbTX-3. Afterwards, Poli and co-workers , who used tritiated PbTX-9 in the development of their assay, observed that both C-CTX-1 and PbTX-3 were approximately equipotent. This RBA was applied to the analysis of fish tissue (from a CFP outbreak in Haiti occurred in 1995), which allowed the quantification of 20 ng PbTx-3 eq/g fish tissue content, confirmed to be C-CTX-1 by LC-MS. Afterwards, as mentioned in the previous section, Dechraoui et al.  used CBA and RBA in parallel in order to differentiate C-CTX-1 from PbTX. It is important to note that, contrary to the work reported by Poli et al. , C-CTX-1 exhibited an 8-fold higher potency in the RBA than brevetoxins. The variability may be due to the receptor source and isolation process. Afterwards, Darius et al.  applied the RBA to the analysis of fish and Gambierdiscus populations from CFP areas in two islands of French Polynesia. In this case, a threshold toxin content was established to differentiate positive from negative fish. Interestingly, while in risky areas (where ciguatera cases are reported annually) 94% of the fish were positive by RBA, Gambierdiscus cells were absent. Contrary, in the area supposed to be safe, fewer fish were positive by RBA (74%) but Gambierdiscus cells were present. The RBA has also been useful to confirm the P-CTX-3C production by Gambierdiscus polynesiensis cultures and to assess variations in the toxic potency of different clones . Despite the relevance of RBA as useful tool for CTX determination, the need for radiolabelled compounds has hampered the progress of these assays. Attempts have been performed to label PbTXs with other moieties such as a chemiluminescent acridinium dye  or different fluorescent ligands , resulting in promising approaches to avoid constraints associated with radioactivity.
Table 3 summarizes the biochemical assays and cell-based assays that have been developed for the detection of CTX.
|Clams sea urchins and cyanobacteria|||
|Rat/mouse blood and urine||[157,158,159,160]|
|Hemolytic assay||Colorimetry||Prorocentrum sp. and fish|||
2.3. Cyclic Imines
Cyclic imines (CIs) are a heterogeneous group of marine lipophilic phycotoxins including: spirolides (SPXs), gymnodimines (GYMs), pinnatoxins (PnTXs), pteriatoxins (PtTXs), prorocentrolides and spiro-prorocentrimines [172,173]. They are macrocyclic compounds with imine (carbon-nitrogen double bond) and spiro-linked ether moieties. They have been grouped together because of their common imine group as a part of a cyclic ring, which confers the pharmacological and toxicological activity, and due to their similar acute “fast acting toxicity” in mice . CIs are produced by different dinoflagellates to a different extent: SPXs are mainly produced by Alexandrium ostenfeldii also known as A. peruvianum [174,175], GYMs are produced by Karenia selliformis, also known as Gymnodinium selliforme . The PnTXs-producing organism has been described as peridinoid dinoflagellate  and recently identified as Vulcanodinium rugosum , prorocentrolides have been isolated from Prorocentrum lima , spiro-prorocentrimines are suggested to be produced by Prorocentrum species  and PtTXs have only been detected in shellfish and no producing organism has been identified .
Nowadays, the growing CIs family found in dinoflagellates and/or in shellfish includes 31 members: 4 GYMs [181,182], 14 SPXs (among which, E and F are non-toxic due to the lack of imine ring) , 7 PnTXs , 3 PtTXs, 2 prorocentrolides and 1 spiro-prorocentrimine [183,185]. Apart from these analogues, some fatty acid acyl esters derivatives, products of shellfish metabolism, have been identified: 21 SPXs  and 26 PnTXs . Among CIs, SPXs are the largest group and together with GYMs are the best characterized. PnTXs and PtTXs are almost structurally identical and they are the CIs most closely related to SPXs. Moreover, PtTXs, prorocentrolides and spiro-prorocentrimine have been recently identified as biotransformation products of PnTxs in shellfish .
The first evidences indicating the presence of SPXs, GYMs and PnTxs were reported in the early 1990s during routine monitoring of bivalve molluscs in Canada , New Zealand  and Japan , respectively. The cyclic imine group in the molecule is assumed to be responsible for the neurotoxicity of these toxins. The mechanism of action of SPXs and GYMs is based on their inhibition of both muscarinic and nicotinic acetylcholine receptors (mAChRs and nAChRs) in the central and peripheral nervous system [36,191]. Nevertheless, while GYMs effect on these receptors is reversible, SPXs binding seems to be irreversible . As regards to PnTXs, it has recently been demonstrated that they also bind to nAChRs . Prorocentrolides and spiro-prorocentrimine molecular targets are still unknown .
Only SPXs, GYMs and PnTXs have been detected in Europe. In addition, there is limited information about the absorption, distribution, metabolism and excretion of CIs in animals or humans, and no intoxication cases have ever been reported . This fact explains the absence of regulatory limits and official analysis methods for CIs in shellfish. However, the working group of the EU-RLMB has proposed a guidance level for the sum of SPXs of 400 µg/kg in shellfish meat [44,193].
Given the novelty of the research area of CIs, it is crucial to better describe their mechanisms of action, as well as to widen the toxicological and pharmacological data in order to determine if they pose a public health risk. This is particularly important considering the widespread distribution of CIs in seafood and their potent binding to nicotinic acetylcholine receptors in the central and peripheral nervous systems and this might have long-term effects on human health. It would also be important to develop suitable methodologies for the detection of all CIs and implement preventive measures in monitoring programmes .
2.3.1. Cell-Based Assays for Cyclic Imines
Even though no CBAs have been developed so far for CIs, there are some studies about mechanism of action, which will set the basis to develop those functional tests in a near future. One of the first evidences that GYM targets muscular and neuronal nAChRs was demonstrated by Kharrat et al. . In this work, frog and mouse nerve muscle preparations were used for electrophysiological studies by means of axonal clamp and tension experiments. GYM-A blocked the twitch response when nerve was stimulated, suggesting that this toxin should be a nAChRs blocker in muscles. Compared to tubocurarine, a 135-fold higher concentration was needed of this toxin to obtain a similar degree of GYM-A blocking. Furthermore, this blocking was concentration- and time-dependent. In order to confirm these findings in native receptors, primary cell cultures of Xenopus laevi embrios myocytes were used in patch clamp experiments, as these myocytes express nAChRs. Extracellular perfusion of GYM-A reduced nicotinic currents elicited by the neurotransmitter in a reversible manner, thus confirming the results obtained in nerve muscles. In addition, studies on how GYM-A affected receptors and subunits affinity were performed. Homomeric neuronalhuman α-7 nAChR were expressed in Xenopus laevis oocytes for electrophysiological studies (patch clamp). Moreover, neuromuscular human heteropentameric and neuronal human chimeric receptors of α-7 nAChR were expressed in HEK293 cells for competition studies. These studies revealed that GYM-A is more effective on chimeric nAChRs neuronal alfa7-5ht3 than on muscular ones, but it was 30 times less effective on homomeric α-7 nAChR expressed in Xenopus oocytes. The inhibitory of GYM-A was similar on different animal tissues but dependent on the nAChR receptor subtype and subunits comprising the receptor.
Dragunow et al.  studied the effects of synthetic GYM, GYM acetate and GYM carbonate on N2a cells. The toxic effect on the viability, although significant compared to the control, was very low. Nevertheless, the authors observed that pre-exposure of cells to these toxins sensitised them to okadaic acid, suggesting that GYM and its analogues may be rendering cells more susceptible to the effects caused by other toxins. However, GYM acetate did not produce stress proteins such as c-Junk, ATF-2 or ATF-3 neither proliferation, thus other mechanisms may be involved in the sensitisation of these toxins.
Another interesting study was performed by Geiger et al. , which used several cytotoxicity assays in order to investigate the mechanisms of action of PnTx-G. No viability inhibition was found on N2a, KB and CaCo2 cells even after 72 h of exposure of the toxin. Nevertheless, fractions obtained from V. rugosum extracts were found to be toxic for these cell lines, suggesting that other compoundscan be implied in the toxicity of V. rugosum.
Finally, Hellyer et al.  have recently synthesized a fluorescent PnTx-F and have applied to muscle sections from thy1-YFP-H transgenic mice, which express yellow fluorescent protein (YFP) in motor nerves, to allow visualization of interactions of this toxin with nicotinic receptors. The addition of spacers and the fluorescent moiety to PnTx-F showed reduced in vitro neuromuscular-blocking potency and in vivo toxicity compared to unmodified PnTx-F. However, despite this reduced potency, the fluorescent toxin efficaciously labelled endplates of mouse muscle motor nerves, retained neuromuscular blocking abilities in vitro, and displayed in vivo toxicity. Consequently, this study is an important step not only to provide with fluorescence assays for toxin detection, but also to understand how toxins and derivatives interact with nicotinic receptors.
Tatters and co-workers  performed a hemolytic study based on Eschbach erythrocyte lysis assay  for ichthyotoxic algae, and quantified the response in relation to saponin. A weak hemolytic activity was observed for the GYM standard, whereas Karenia selliformes reported a high response. Hemolytic assay appeared to be a non-sensitive method for GYM detection.
2.3.2. Receptor-Binding Assays for Cyclic Imines
The birth of biochemical methods for the detection of CIs is very recent. The first work was a FP indirect assay in solution for the detection of GYM-A and 13-desmethyl C (13-DesMeC) SPX . The authors developed a competitive inhibition assay based on the affinity of these toxins towards nAChRs, purified from Torpedo marmorata electrocyte membranes, and using fluorescent α-bungarotoxin (BTX) as a competitive tracer. The assay provided IC50 values of 390 nM and 108 nM for GYM-A and 13-DesMeC SPX, respectively. Matrix effects were evaluated using mussel samples, which only shifted slightly the IC50 values to 281 and 129 nM, respectively. The higher sensitivity, and thus affinity, of SPX towards the receptors compared to GYM, suggests an also higher toxicity. The same authors also demonstrated the suitability of this assay to the quantification of 13,19-didesmethyl C (13,19-diDesMeC) SPX, with a sensitivity similar to that of 13-DesMeC SPX, and negligible matrix effects . The applicability of this assay to clams, cockles and scallops has also been demonstrated . Spiked shellfish extracts provided IC50 values of around 100 nM for SPXs and between 300 and 400 nM for GYMs. Otero et al.  labelled nAChRs with a derivative of fluorescein to develop a FP direct assay for the quantification of SPX. The approach provided appropriate LODs for 13-DesMeC SPX and 13,19-diDesMeC SPX (25 and 150 nM, respectively). The shift from indirect to direct recognition implied a significant improvement of sensitivity and rapidity.
Based on the same sensing principle, solid-phase RBAs with different detection techniques have been developed [204,205,206,207]. Rodríguez and co-workers  developed an indirect assay based on a competition between biotin-labelled α-BTX and CIs for their binding to nAChRs in solution, and the subsequent immobilisation of the α-BTX-receptor complex on streptavidin-coated surfaces. The amount of immobilised receptor was measured by using an anti-nAChR Ab and an HRP-labelled secondary Ab. The use of three different substrates for HRP allowed adapting the assay to chemiluminescence, fluorescence and colorimetric detection techniques. The three end-points yielded similar calibration curves for 13-DesMeC SPX (IC50 ≈ 35 nM and LOD (IC20) ≈ 10 nM), although the chemiluminescence substrate seem to be slightly better. Moreover, no matrix interferences were observed when using cockle extracts. The chemiluminescence approach was also applied to the detection of GYM, being 10 times less sensitive than with 13-DesMeC, possibly due to the lower GYM toxicity. The same authors developed a similar assay, but first immobilising the biotin-labelled α-BTX on streptavidin-coated plates and then carrying out the competition step and the primary and secondary Ab incubations . The assay was successfully applied to the determination of 13-DesMeC SPX, 13,19-diDesMeC and 20-MetG (IC50 = 38, 18 and 55 nM and LOD (IC10) = 11, 8 and 16 nM, respectively), the first one also tested in scallops. Additionally, the assay was optimized in 384-well plates for high-throughput screening.
The immobilisation of the receptors instead of the α-BTX has also been exploited for the development of two assays [206,207]. In the first work, the competition takes place between biotin-labelled α-BTX and 13,19-diDesMeC, 13-desMeC, PnTX-G, PnTX-A, GYM-A or AK-PnTX-A for binding to nAChRs immobilised on microtiter plates, and the colorimetric detection is measured using streptavidin-HRP . In order to address the lack of selectivity of the assay, the toxins bound to nAChRs were eluted from the wells and analyzed by mass spectrometry to determine their chemical structure. In the second work, nACHRsor AChBPs (binding proteins) were immobilised on carboxylated microspheres and the subsequent binding competition between biotin-labelled α-BTX and 13-DesMeC was measured by flow cytometry using fluorescent phycoerythrin (PE)-labelled streptavidin . Both receptors allowed the detection of 13-desMeC SPX, 13,19-DesMeC SPX and 20-MetG SPX and GYM with different sensitivities but always in the nanomolar range, attaining even an LOD as low as 0.05 nM for 13-desMeC SPX in scallops.
Table 4 summarizes the biochemical assays and cell-based assays that have been developed for the detection of CIs.
|Patch clamp (electrophisiology)||-|||
|Hemolytic assay||Colorimetry||Karenia selliformis|||
|Clams, oysters, scallops and mussels|||
|Fluorescence (coupled to flow cytometry)||Scallops|||
|Clams, cockles and scallops|||
Tetrodotoxin (TTX) is one of the most potent low-molecular-weight marine neurotoxins (319 Da). Its chemical structure was described as a cage-like polar molecule with a cyclic guanidinium moiety fused to a dioxy-adamantane skeleton embellished by six hydroxyl groups. To date, 29 different analogues of TTX have been reported. Their degree of toxicity varies among analogues, although not much is known about them . An important recognized feature is that the deoxy analogues of TTX are less toxic than TTX, while the hydroxyl analogues are more toxic than TTX .
TTX was first discovered in 1909 by Tahara in ovaries of globefish  and its structure was more understood in 1950 with the isolation of the crystalline form of TTX from the ovaries of puffer fish (Fugu rubripes) . However, the complete structure was only known through the findings of three independent groups [212,213,214]. Puffer fish seems to accumulate the toxin in the gonads, liver and skin . Later on, the presence of TTX has been reported in many invertebrate species, such as starfish, blue-ring octopus, xanthid crabs, gastropod and flatworm, and in vertebrate species, including frogs, goby and newt Taricha . TTX is produced by certain endo-symbiotic bacteria, such as Vibrio sp., Pseudomonas sp. and Alteromonas .
Like saxitoxin (STX), TTX has the ability to selectively bind to VGSCs, blocking both nerve and muscle action potentials. Contrary to CTX, TTX closes the channels. This blocking affects the passive influx of sodium ions, resulting in numbness, respiratory paralysis, mild gastrointestinal effects, and even death of human consumers. Consequently, bioaccumulation of TTX in seafood and subsequent entrance in the human food chain poses a real risk to human safety.
The Japanese government has established a regulatory limit of 2 mg/kg of TTX equivalents in food, due to “fugu” consumption in Japan. In contrast, no regulatory limits have been set in Europe because TTX poisoning had not been a problem. However, in October 2007, the first toxic European episode were reported in Malaga (Spain), caused by the ingestion of a trumpet shell of the species Charonia lampas lampas. The product was purchased in a Malaga market, but it was caught in the south coast of Portugal . Again, in 2005 and 2008, puffer fish migrating from the Red Sea to the Mediterranian Sea due to the Lessepsian phenomenon, were responsible for human poisoning, although low levels of TTX were found in liver and gonades . Other seafood species such as molluscs and echinoderms were caught in the Portugal coast and have also been described to contain TTX . Therefore, European seafood is endangered of being contaminated with this hazard toxin, which highlights the need to have a regulation and to develop unambiguous, fast and reliable methods to specifically detect and quantify TTX in order to protect human health.
2.4.1. Immunoassays and Immunosensors for Tetrodotoxins
Like with CIs, in the development of immunoassays for TTX, production of Ab is a bottleneck because of the small size of the toxin and the need to conjugate it to a carrier protein in order to get animal immunisation. Ideally, the carrier protein to be used as a coating agent in the ELISA should be different from that used for animal immunisation in order to avoid cross-reactivity of the Ab against the protein . As an example, TTX-BSA conjugate has been synthesized for MAb production and TTX-OVA conjugate has been used as a coating agent in ELISA , and vice versa [222,223]. The developed competitive ELISAs have attained proper LODs, e.g., 2 [221,223,224], 5  and 10 ng/mL , regardless the experimental conditions and formats.
Another important feature in immunoassays is the cross-reactivity towards analogues. Not much work has been performed in this direction, due to the fact that standards are not commercially available. Although some TTX derivatives may be chemically synthesized or extracted and purified from naturally-contaminated material, the cross-reactivity values may not be accurate. Nevertheless, useful qualitative information has been provided. Kawatsu and co-workers  reported no cross-reactivity of their Ab towards gonyautoxins or tetrodonic acid, and minor cross-reactivity towards anhydro-tetrodotoxin. In fact, the cross-reactivity of an Ab depends on the functional group used as a site to conjugate the toxin to the carrier protein. The observed results are consistent with the fact that the guanidinium group was used to conjugate TTX to BSA.
Some direct ELISA formats have been developed in order to reduce analysis times. Neagu and co-workers  produced a TTX-ALP tracer to use in an ELISA with immobilised anti-TTX MAb. The Ab was still able to recognize the tracer, indicating that the conjugation did not affect much the affinity event. Nevertheless, it is necessary to mention that, even though the detection was direct, a secondary Ab (non-labelled) was used to improve the anti-TTX MAb orientation and, therefore, the analysis time was still compromised. Other works have conjugated HRP [221,227] or ALP [223,228] to the anti-TTX antibody to avoid the use of a labelled secondary Ab. Despite the advantages of using conjugates, their stability (of the linkage and of the enzyme activity) still is a pending task. Recombinant Ab fragments have also been synthesized through phage display technology, and used in the development of ELISAs for TTX determination . However, in order to achieve high sensitivities, more work needs to be undertaken.
Some of these direct ELISA configurations have been used in the development of electrochemical immunosensors on screen-printed electrodes. In the approach with the TTX-ALP tracer , differential pulse voltammetry (DPV) was used to measure the α-naphthol produced by the reaction between the substrate α-naphthyl phosphate and the enzyme label, decreasing the LOD from 2 to 1 ng/mL (compared to the colorimetric approach). In the approach with labelled primary Ab , based on the amperometric detection of the p-aminophenol at +300 mV vs. Ag/AgCl, product of the reaction between p-aminophenyl phosphate and ALP, an LOD of 0.016 ng/mL was attained, one of the lowest limits ever reported.
Immunochromatographic strips have also been developed with the aim of providing a rapid visual test for the screening of TTX [229,230,231]. These assays, prepared on an absorbent pad to favor lateral flow, are based on the competition between the TTX from the sample and the TTX-BSA immobilized on the test line for a colloidal gold-labelled anti-TTX Ab. The visual LOD attained with this format using spiked puffer fish samples (muscle and gonad) was 40 ng/mL of TTX [229,230]. Although this method is not quantitative, it has the advantages of simplicity, ease of use, no sophisticated equipment requirement and short analysis times (10 min). This test has also been used in the screening of fish, establishing an action limit of 2 mg/kg . The comparison with LC-MS/MS analysis has demonstrated the applicability of strip tests to the analysis of naturally-contaminated fish samples.
Several SPR immunosensors for the detection of TTX based on direct approaches have been reported [232,233,234,235,236,237]. Due to the small size of the toxin, it is very difficult to directly detect its interaction with immobilised Ab and, consequently, the appropriate configuration involves antigen immobilisation and detection of the Ab interaction. In the first works [232,233,234,235], the gold SPR chip was functionalised with a mixed self-assembled monolayer (SAM) of hydroxy- and amino-terminated oligo-ethylene glycol alkanethiols (OEG-ATs), since the ethylene glycol units were known to minimize non-specific protein adoption. Whilst amino-terminated OEG-ATs were used to covalently link the TTX to the surface with formaldehyde, hydroxy-OEG-ATs were used as spacer molecules to avoid cross-linking between amino-OEG-ATs. TTX was also linked using reductive alkylation but, as expected, higher Ab recognition yields were observed when using formaldehyde because the reaction site (the guanidine group) was the same as in the Ab production. TTX has also been immobilised directly on a carboxymethylated chip, which simplifies the protocol and still retains the performance . Like in colorimetric competition immunoassays, the MAb concentration was optimized taking into account that surface saturation is not desired, since low TTX concentrations would be more difficult to detect. These optical immunosensors attained a LOD (IC20) as low as 0.3 ng/mL . It is evident that the most exhaustive applicability studies have been performed with SPR immunosensors. The analysis of matrix effects from pufferfish liver and muscle, gastropods, and urine, is of utmost importance in food safety and clinical applications. Although the presence of a natural matrix may increase the LOD, this effect can be minimized by properly modifying the assay running buffer . Several experimental parameters, such as pH, concentration and BSA presence, can be modulated to avoid non-specific adsorption while maintaining an appropriate Ab interaction and LOD. The analysis of naturally-contaminated fish has always shown appropriate correlations with LC/MS analysis [51,234]. An additional advantage of these systems is the ability to regenerate the surface for continuous TTX determinations. A pre-validation study was performed in parallel by three independent laboratories using spiked and naturally-contaminated pufferfish samples . Although the method could be improved and the comparison was only a proof of concept, it is reasonable to conclude that SPR immunosensors are very promising as analysis tools for TTX quantification.
The SAM immobilisation strategy has also been used in the development of another optical immunoassay for TTX . In this case, the chip was inserted into a microfluidic cell coupled to a microscope and a camera, and the interaction was monitored by imaging using a microbead-coated secondary Ab. Controlled fluidic forces were applied to remove non-specifically bound beads by fluidic force discrimination (FFD). The LOD for TTX was 15 ng/mL and a large dynamic range (4–5 orders of magnitude) was obtained.
Recently, Yakes and co-workers  have developed a non-competitive SPR immunosensor, immobilising anti-TTX MAb on the surface of the SPR chip instead of TTX. This new configuration permits the direct detection of free TTX, which is a challenge taking into account its low molecular weight. This has been possible thanks to the recent advances in SPR instrumentation: lower noise detection systems, improved fluidics with stronger vacuum pumps and lower noise valves. Antibody kinetics and cross-reactivity studies towards other co-occurring toxins with a similar mode of action were performed, which demonstrated the selectivity of the antibody, since no competitive or additive effects were observed. This strategy has allowed lowering the LOD with respect to the competitive SPR biosensors to 0.09 ng/mL of TTX and could, in a future, be extended to the detection of other small molecules.
2.4.2. Cell-Based Assays and Biosensors for Tetrodotoxins
The first N2a CBA for TTX (and also STX) detection was developed by Kogure and co-workers , and was initially named tissue culture-based assay (TCBA) because of the cell tissue layer created on the well. Previous studies had suggested that TTX blocked conduction of nerve and muscle through selective inhibition of the sodium-carrying mechanism [240,241]. The assay was thus based on the ability of TTX to block VGSCs and rescue cells from the effect of ouabain (which inhibits the Na+/K+-ATPase pump) and veratridine (which causes sodium ion influx into the cells), counteracting the toxic antagonist effect of veratridine and increasing the viability of cells. In that assay, viability was measured by the morphological changes of the cells with an optical microscope, attaining an LOD of 3 nM of TTX. The assay was applied to the analysis of strains of bacteria from marine sediments. The applicability of the assay to freshwater sediments was also demonstrated , indicating that TTX can be produced by freshwater bacteria and accumulate in lakes and ponds sediments. The assay has also been useful to screen the TTX production by bacteria isolated from the ovaries of pufferfish .
In order to get more accurate quantifications, the assay has been improved by specific staining of the cells with dyes, which estimate the relative abundance of living cells by absorbance measurements. With this aim, neutral red  and tetrazolium salts such as WST-1  and MTT  have been used, these salts avoiding the washing steps thus providing greater reproducibility. The IC50 values attained with these dyes were 50, 6.6 and 12.9 nM, respectively.
When investigating the applicability of the CBA for TTX; it is necessary to keep in mind that this assay is not specific for TTX but can also detect other VGSC blockers such as STX and gonyautoxins; which could co-occur with TTX in the same sample [247,248,249]. Nevertheless; this CBA has demonstrated to be useful as a screening method. In addition, of interest is the application of CBAs to assess the toxicity of TTX analogues .
Following the same principle but a different measurement technique, a biosensor for TTX detection has been developed . This biosensor consisted of a sodium electrode covered with frog bladder membrane integrated within a flow cell. The concentration of TTX was measured from the inhibition ratio of the sensor peak output. When naturally-contaminated puffer fish samples were analyzed, linear correlations were obtained in the comparison with MBA, the biosensor being able to detect TTX at concentrations far below the LOD of the MBA (1 µg/g). Again, the biosensor was also able to detect STX and GTX analogues .
In a more sophisticated work, a portable microelectrode array incorporating neuronal networks has been developed for TTX detection [252,253]. The system was able to monitor extracellular potentials from spinal cord cells cultured on electrodes and exposed to TTX, attaining an IC50 of 4 nM of TTX. Similar is the work performed by Mohan and co-workers , who used patch clamp electrophysiology to record the action potentials caused by the effect of TTX on NG108-15 cells. In this case, experimental data were used to create a computer model. Despite the fact that the toxic effect of TTX was observed, more efforts are needed to provide a proper calibration curve and to refine the mathematical model. Comparison between electrophysiological recordings and a CBA with a fluorescent dye and has been performed , using recombinant subunits expressed by Chinese hamster ovary (CHO) cells. There was a linear relationship between the log IC50 values obtained by the two methods for different VGSC modulators.
Like for CTX, the hemolytic capacity of TTX has been exploited for the development of a screening assay , based on the N2a CBA for CTX  and the heamolytic assay for PLTX . In this test veratridine was used as TTX antagonist. The hemolytic assay was performed with red tilapia RCBs. This fish has the capacity to regulate his body ion concentration to a wide range of salinities. More experimentation is required to find minimal concentration of TTX needed to inhibit hemolysis reactive concentrations for a valid detection. Table 5 summarizes the biosensors, biochemical assays and cell-based assays that have been developed for the detection of TTX.
|Milk and apple juice|||
|Immunostick test||Visual||Spiked puffer fish||[229,230]|
|Bacteria from freshwater sediments|||
|Bacteria from pufferfish|||
|Bacterial culture supernatants||[244,245]|
|Spiked E. coli|||
|Patch clamp |
3. Conclusions and Future Perspectives
When available, the instrumental analysis approach for the quantification of emerging toxins is suitable for the unambiguous quantification of analytes. However, if the potency of each analogue has not been described (i.e., toxicity factors between analogues are not well established), the instrumental analysis methods may not be the right solution to estimate toxicity and risk. As MBAs are controversial, alternative methods providing functional or toxicological information have a role to play in the identification of risks, as a complement to instrumental analysis approaches. Alternative methods based on structural recognition can also contribute to quantify overall presence of analogues of a group of toxins. Therefore, alternative methods, once validated, may become excellent screening tools and even contribute to quantify toxins or restricted groups of toxins. Instrumental analysis methods may be applied to obtain more precise quantifications or toxin profiles in positive samples identified by screening assays.
Whereas some alternative methods for the detection of classical marine toxins have even reached the market (e.g., protein phosphatase inhibition assay (PPIA) for okadaic acid and ELISA for domoic acid), those for emerging marine toxins, although successful, have not been optimized nor validated, and those few that have reached the market, such as the immunostrip tests for CTXs, have suffered from inefficiencies and controversy. A reason for this may be that not enough is known about the toxicity and mechanism of action of some emerging toxins, this being especially important when considering families of toxins with numerous analogues. This scarce information, together with the lack of certified standards and reference materials, are responsible for the underdevelopment of analysis methods. As a consequence, official methods and MPLs of these toxins in seafood may not have been established yet. Additionally, for some of these toxins, toxicological evidence is not available yet and thus, regulatory institutions cannot adopt appropriate regulations.
Each alternative method has advantages and drawbacks. The interaction of toxins with antibodies is based on a structural recognition, thus not necessarily related to toxicity. However, antibodies are still robust biorecognition molecules, with high affinity and sensitivity towards their analytes, sometimes being even able to detect different analogues of the same group of toxins. Moreover, their easy handling and manipulation allow their integration into different assay formats, being adaptable to the different end users. Like antibodies, receptors specifically bind to their corresponding ligands on a structural recognition basis. The replacement of the commonly used radioactive labels by optical ones has favored the spreading of RBAs. However, they still suffer from complex setups and the requirement of the isolation of receptors from a variety of animals. Cells provide an indication of the overall toxicity, and have the advantage to be closer to animal models than antibodies and receptors. The use of human cell models can be even more useful to extrapolate some effects on human tissues. However, working with “live” material implies certain degree of variability, both inter and intra cell lines. Environmental conditions, cell status or cell-cycle stage can also lead to variations in the responses. Another limitation is the impossibility or difficulty to identify or discriminate compounds that share the same mechanism of action. The effects from the seafood matrices on the assay or biosensor are also of concern in any detection method.
It is evident that the alternative methods described in this review are promising tools to identify and quantify emerging marine toxins. Some of them have even been demonstrated to be highly performing and applicable to the analysis of naturally-contaminated samples, and when limitations have been identified, efforts have been made to overcome them. As an example, sample fractioning can separate toxins according to their chromatographic retention time and thus, contribute to discriminate them. As another example, matrix effects can be minimized by modifying toxin extraction protocols or adding previous sample clean-up steps. However, attempts to optimize, validate and standardise these methods have been scarce, so far. Now, it is about time to make efforts to solve the pending tasks. The combination of screening tools and confirmatory instrumental analysis methods should be the solution to achieve highly specific, sensitive and fast routine monitoring of emerging marine toxins. Additionally, research has to pursue to better describe mechanisms of action of emerging toxins, to identify or produce further recognition molecules, and to develop sample purification methods in order to promote the implementation of alternative methods. It is also necessary to take into account that these alternative methods will be used by laboratories performing environmental and food safety controls, food producers, fishermen and other end-users. Consequently, their formats should be friendly and easy to use. The development of kits is certainly a sound and reachable goal, as it has happened with some regulated toxins (PSP, ASP and some lipophilic toxins). However, for some experimental approaches such as CBAs, the development of kits may not be appropriate, and in this case the priority would be the coordination of laboratories in order to harmonise and validate the methodologies. Biosensors are a very promising approach since they have demonstrated to be very specific and robust, to attain very low limits of detection, and to conduct analyses in short times. Depending on the type and the format, they may provide additional advantages such as real-time responses and, when miniaturisation is possible, device portability for in situ measurements. Multi-disciplinary and cooperative work would certainly speed up and strengthen the progress in this area.
Mònica Campàs acknowledges financial support from the Ministerio de Ciencia e Innovación and the European Social Fund through the Ramón y Cajal program and the Ministerio de Economía y Competitividad for the DIANA project (BIO2011-26311). Laia Reverté acknowledges the ECsafeSEAFOOD project (grant agreement number 311820) of the European Union Seventh Framework Programme (FP7/2007-2013), Lucía Soliño the Universitat Rovira i Virgili, Banco de Santander and IRTA, and O.C. the XRAq—Xarxa de Referència d’R+D+I en Aqüicultura de la Generalitat de Catalunya, for the respective PhD grants.
Laia Reverté and Mònica Campàs drafted and organised the manuscript. Laia Reverté focused on the general information about the toxins and on biosensors and biochemical assays, Lucía Soliño on cell-based assays and Olga Carnicer on haemolytic assays. Jorge Diogène and Mònica Campàs focused on the introduction and conclusions and also extensively reviewed and contributed in all sections. All authors did literature search, and read and approved the final manuscript.
Conflicts of Interest
The authors declare no conflict of interest.
- European Food Safety Authority (EFSA). Scientific Opinion on marine biotoxins in shellfish: Cyclic imines (spirolides, gymnodimines, pinnatoxins and pteriatoxins). EFSA J. 2010, 8. [Google Scholar] [CrossRef]
- European Food Safety Authority (EFSA). Scientific Opinion on marine biotoxins in shellfish—Emerging toxins: Ciguatoxin group. EFSA J. 2010, 8. [Google Scholar] [CrossRef]
- European Food Safety Authority (EFSA). Scientific opinion on marine biotoxins in shellfish-palytoxin group. EFSA J. 2009, 7, 1–38. [Google Scholar]
- European Comission. Regulation (EC) No 854/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific rules for the organisation of official controls on products of animal origin intended for human consumption. Off. J. Eur. Union 2004, L226, 83–127. [Google Scholar]
- European Comission. Regulation (EC) No. 853/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for food of animal origin. Off. J. Eur. Union 2004, L139, 22–82. [Google Scholar]
- European Comission. Regulation (EU) No 15/2011 of 10 January 2011 amending Regulation (EC) No 2074/2005 as regards recognised testing methods for detecting marine biotoxins in live bivalve molluscs. Off. J. Eur. Union 2011, L6, 3–6. [Google Scholar]
- Foodstuffs—Determination of domoic acid in shellfish and finfish by RP-HPLC using UV detection. Available online: http://aesan.msssi.gob.es/CRLMB/docs/docs/procedimientos/Foodstuffs.pdf (accessed on 11 November 2014).
- Lawrence, J.F.; Niedzwiadek, B.; Menard, C. Quantitative determination of paralytic shellfish poisoning toxins in shellfish using prechromatographic oxidation and liquid cromatography with fluorescence detection: Collaborative Study. J. AOAC Int. 2005, 88, 1714–1732. [Google Scholar]
- Van de Riet, J.M.; Gibbs, R.S.; Chou, F.W.; Muggah, P.M.; Rourke, W.A.; Burns, G.; Thomas, K.; Quilliam, M.A. Liquid chromatographic post-column oxidation method for analysis of paralytic shellfish toxins in mussels, clams, scallops, and oysters: Single-Laboratory Validation. J. AOAC Int. 2009, 92, 1690–1704. [Google Scholar]
- Van de Riet, J.; Gibbs, R.S.; Muggah, P.M.; Rourke, W.A.; MacNeil, J.D.; Quilliam, M.A. Liquid chromatography post-column oxidation (PCOX) method for the determination of paralytic shellfish toxins in mussels, clams, oysters, and scallops: Collaborative study. J. AOAC Int. 2011, 94, 1154–1176. [Google Scholar]
- Turner, A.D.; Lewis, A.M.; Rourke, W.A.; Higman, W.A. Inter laboratory comparison of two AOAC liquid chromatographic fluorescence detection methods for paralytic shellfish toxin analysis through characterization of an oyster reference material. J. AOAC Int. 2014, 97, 380–390. [Google Scholar] [CrossRef]
- Gerssen, A.; Mulder, P.; McElhinney, M.A.; de Boer, J. Liquid chromatography-tandem mass spectrometry method for the detection of marine lipophilic toxins under alkaline conditions. J. Chromatogr. A 2009, 1216, 1421–1430. [Google Scholar]
- Fux, E.; McMillan, D.; Bire, R.; Hess, P. Development of an ultra-performance liquid chromatography-mass spectrometry method for the detection of lipophilic marine toxins. J. Chromatogr. A 2007, 1157, 273–280. [Google Scholar]
- McNabb, P.; Selwood, A.I.; Holland, P.T. Multiresidue method for determination of algal toxins in shellfish: Single-laboratory validation and interlaboratory study. J. AOAC Int. 2005, 88, 761–772. [Google Scholar]
- Takada, N.; Umemura, N.; Suenaga, K.; Chou, T.; Nagatsu, A.; Haino, T.; Yamada, K.; Uemura, D. Pinnatoxins B and C, the most toxic components in the pinnatoxin series from the Okinawan bivalve Pinna muricata. Tetrahedron Lett. 2001, 42, 3491–3494. [Google Scholar] [CrossRef]
- Miles, C.O.; Rundberget, T.; Sandvik, M.; Aasen, J.; Selwood, A.I. The Presence of Pinnatoxins in Norwegian Mussels; National Veterinary Institute: Oslo, Norway, 2010. [Google Scholar]
- Lewis, R.J.; Jones, A.; Vernoux, J.-P. HPLC/Tandem Electrospray Mass Spectrometry for the determination of sub-ppb levels of Pacific and Caribbean ciguatoxins in crude extracts of fish. Anal. Chem. 1998, 71, 247–250. [Google Scholar]
- Hamilton, B.; Hurbungs, M.; Jones, A.; Lewis, R.J. Multiple ciguatoxins present in Indian Ocean reef fish. Toxicon 2002, 40, 1347–1353. [Google Scholar] [CrossRef]
- Munday, R. Palytoxin toxicology: Animal studies. Toxicon 2011, 57, 470–477. [Google Scholar] [CrossRef]
- Moore, R.E.; Scheuer, P.J. Palytoxin: A new marine toxin from a coelenterate. Science 1971, 172, 495–498. [Google Scholar] [CrossRef]
- Walsh, G.E.B.; Ralph, L. A review of Hawaiian zoanthids with descriptions of three new species. Zool. J. Linn. Soc. 1971, 50, 161–180. [Google Scholar] [CrossRef]
- Moore, R.E.; Bartolini, G. Structure of palytoxin. J. Am. Chem. Soc. 1981, 103, 2491–2494. [Google Scholar] [CrossRef]
- Uemura, D.; Ueda, K.; Hirata, Y.; Naoki, H.; Iwashita, T. Further studies on palytoxin. II. Structure of palytoxin. Tetrahedron Lett. 1981, 22, 2781–2784. [Google Scholar] [CrossRef]
- Ciminiello, P.; Dell’Aversano, C.; Dello Iacovo, E.; Fattorusso, E.; Forino, M.; Grauso, L.; Tartaglione, L.; Florio, C.; Lorenzon, P.; de Bortoli, M. Stereostructure and biological activity of 42-hydroxy-palytoxin: A new palytoxin analogue from Hawaiian Palythoa subspecies. Chem. Res. Toxicol. 2009, 22, 1851–1859. [Google Scholar] [CrossRef]
- Uemura, D.; Hirata, Y.; Iwashita, T.; Naoki, H. Studies on palytoxins. Tetrahedron 1985, 41, 1007–1017. [Google Scholar] [CrossRef]
- Inuzuka, T.; Uemura, D.; Arimoto, H. The conformational features of palytoxin in aqueous solution. Tetrahedron 2008, 64, 7718–7723. [Google Scholar] [CrossRef]
- Mercado, J.A.; Rivera-Rentas, A.L.; Gonzalez, I.; Tosteson, T.R.; Molgó, J.; Escalona de Motta, G. Neuro- and myo-toxicity of extracts from the benthic dinoflagellate Ostreopsis lenticularis is sensitive to μ-conotoxin. Soc. Neurosci. 1994, 20, 718. [Google Scholar]
- Ukena, T.; Satake, M.; Usami, M.; Oshima, Y.; Naoki, H.; Fujita, T.; Kan, Y.; Yasumoto, T. Structure elucidation of ostreocin D, a palytoxin analog isolated from the dinoflagellate Ostreopsis siamensis. Biosci. Biotechnol. Biochem. 2001, 65, 2585–2588. [Google Scholar] [CrossRef]
- Ukena, T.; Satake, M.; Usami, M.; Oshima, Y.; Fujita, T.; Naoki, H.; Yasumoto, T. Structural confirmation of ostreocin-D by application of negative-ion fast-atom bombardment collision-induced dissociation tandem mass spectrometric methods. Rapid Commun. Mass Spectrom. 2002, 16, 2387–2393. [Google Scholar] [CrossRef]
- Lenoir, S.; Ten-Hage, L.; Turquet, J.; Quod, J.P.; Bernard, C.; Hennion, M.C. First evidence of palytoxin analogues from an Ostreopsis mascarenensis (Dinophyceae) benthic bloom in Southwestern Indian Ocean. J. Phycol. 2004, 40, 1042–1051. [Google Scholar] [CrossRef]
- Tubaro, A.; Durando, P.; del Favero, G.; Ansaldi, F.; Icardi, G.; Deeds, J.R.; Sosa, S. Case definitions for human poisonings postulated to palytoxins exposure. Toxicon 2011, 57, 478–495. [Google Scholar] [CrossRef]
- Milandri, A.; Ceredi, A.; Riccardi, E.; Gasperetti, L.; Susini, F.; Casotti, M.; Faiman, L.; Pigozzi, S. Impact of Ostreopsis Ovata on Marine Benthic Communities: Accumulation of Palytoxins in Mussels, Sea Urchins and Octopuses from Italy, Proceedings of the ICHA14 Conference, Crete, Greek, 1–5 November 2010; pp. 1–5.
- Ciminiello, P.; Dell’Aversano, C.; Fattorusso, E.; Forino, M.; Tartaglione, L.; Grillo, C.; Melchiorre, N. Putative palytoxin and its new analogue, Ovatoxin-a, in Ostreopsis ovata collected along the Ligurian Coasts during the 2006 toxic outbreak. J. Am. Soc. Mass Spectrom. 2008, 19, 111–120. [Google Scholar] [CrossRef]
- Ciminiello, P.; Dell’Aversano, C.; Dello Iacovo, E.; Fattorusso, E.; Forino, M.; Grauso, L.; Tartaglione, L.; Guerrini, F.; Pistocchi, R. Complex palytoxin-like profile of Ostreopsis ovata. Identification of four new ovatoxins by high-resolution liquid chromatography/mass spectrometry. Rapid Commun. Mass Spectrom. 2010, 24, 2735–2744. [Google Scholar]
- Ciminiello, P.; Dell’Aversano, C.; Dello Iacovo, E.; Fattorusso, E.; Forino, M.; Tartaglione, L.; Battocchi, C.; Crinelli, R.; Carloni, E.; Magnani, M.; et al. Unique toxin profile of a Mediterranean Ostreopsis cf. ovata Strain: HR LC-MSn characterization of ovatoxin-f, a new palytoxin congener. Chem. Res. Toxicol. 2012, 25, 1243–1252. [Google Scholar]
- Munday, R. Occurrence and toxicology of palytoxins. Food Sci. Technol. 2008, 173, 693–713. [Google Scholar]
- Aligizaki, K.; Katikou, P.; Milandri, A.; Diogène, J. Occurrence of palytoxin-group toxins in seafood and future strategies to complement the present state of the art. Toxicon 2011, 57, 390–399. [Google Scholar] [CrossRef]
- Hilgemann, D.W. From a pump to a pore: How palytoxin opens the gates. Proc. Natl. Acad. Sci. USA 2003, 100, 386–388. [Google Scholar] [CrossRef]
- Artigas, P.; Gadsby, D.C. Na+/K+-pump ligands modulate gating of palytoxin-induced ion channels. Proc. Natl. Acad. Sci. USA 2003, 100, 501–505. [Google Scholar] [CrossRef]
- Shimahara, T.; Molgó, J. Palytoxin enhances quantal acetylcholine release from motor nerve terminals and increases cytoplasmic calcium levels in a neuronal hybrid cell line. Life Sci. Adv. Pharmacol. 1990, 9, 785–792. [Google Scholar]
- Satoh, E.; Ishii, T.; Nishimura, M. Palytoxin-induced increase in cytosolic-free Ca2+ in mouse spleen cells. Eur. J. Pharmacol. 2003, 465, 9–13. [Google Scholar] [CrossRef]
- Deeds, J.R.; Schwartz, M.D. Human risk associated with palytoxin exposure. Toxicon 2010, 56, 150–162. [Google Scholar] [CrossRef]
- Onuma, Y.; Satake, M.; Ukena, T.; Roux, J.; Chanteau, S.; Rasolofonirina, N.; Ratsimaloto, M.; Naoki, H.; Yasumoto, T. Identification of putative palytoxin as the cause of clupeotoxism. Toxicon 1999, 37, 55–65. [Google Scholar] [CrossRef]
- CRLMB (Community Reference Laboratory for Marine Biotoxins). Report on toxicology working group meeting, 24–25 October 2005; CRLMB: Cesenatico, Italy; pp. 24–25.
- Bignami, G.S.; Raybould, T.J.G.; Sachinvala, N.D.; Grothaus, P.G.; Simpson, S.B.; Lazo, C.B.; Byrnes, J.B.; Moore, R.E.; Vann, D.C. Monoclonal antibody-based enzyme-linked immunoassays for the measurement of palytoxin in biological samples. Toxicon 1992, 30, 687–700. [Google Scholar] [CrossRef]
- Levine, L.; Fujiki, H.; Gjika, H.B.; Vanvunakis, H. Production of antibodies to palytoxin—neutralization of several biological properties of playtoxin. Toxicon 1987, 25, 1273–1282. [Google Scholar] [CrossRef]
- Frolova, G.; Kuznetsova, T.; Mikhailov, V.; Elyakov, G. An enzyme linked immunosorbent assay for detecting palytoxin-producing bacteria. Russ. J. Bioorg. Chem. 2000, 26, 285–289. [Google Scholar] [CrossRef]
- Zamolo, V.A.; Valenti, G.; Venturelli, E.; Chaloin, O.; Marcaccio, M.; Boscolo, S.; Castagnola, V.; Sosa, S.; Berti, F.; Fontanive, G.; et al. Highly sensitive electrochemiluminescent nanobiosensor for the detection of palytoxin. ACS Nano 2012, 6, 7989–7997. [Google Scholar] [CrossRef]
- Boscolo, S. Sandwich ELISA assay for the quantitation of palytoxin and its analogs in natural samples. Environ. Sci. Technol. 2013, 47, 2034–2042. [Google Scholar] [CrossRef]
- Garet, E.; Cabado, A.G.; Vieites, J.M.; Gonzalez-Fernandez, A. Rapid isolation of single-chain antibodies by phage display technology directed against one of the most potent marine toxins: Palytoxin. Toxicon 2010, 55, 1519–1526. [Google Scholar] [CrossRef]
- Yakes, B.; DeGrasse, S.; Poli, M.; Deeds, J. Antibody characterization and immunoassays for palytoxin using an SPR biosensor. Anal. Bioanal. Chem. 2011, 400, 2865–2869. [Google Scholar] [CrossRef] [PubMed]
- Wattenberg, E.V. Modulation of protein kinase signaling cascades by palytoxin. Toxicon 2011, 57, 440–448. [Google Scholar] [CrossRef]
- Bellocci, M.; Ronzitti, G.; Milandri, A.; Melchiorre, N.; Grillo, C.; Poletti, R.; Yasumoto, T.; Rossini, G.P. A cytolytic assay for the measurement of palytoxin based on a cultured monolayer cell line. Anal. Biochem. 2008, 374, 48–55. [Google Scholar] [CrossRef]
- Pawlowiez, R.; Darius, H.T.; Cruchet, P.; Rossi, F.; Caillaud, A.; Laurent, D.; Chinain, M. Evaluation of seafood toxicity in the Australes archipelago (French Polynesia) using the neuroblastoma cell-based assay. Food Add. Contam. A 2013, 30, 567–586. [Google Scholar] [CrossRef]
- Kerbrat, A.S.; Amzil, Z.; Pawlowiez, R.; Golubic, S.; Sibat, M.; Darius, H.T.; Chinain, M.; Laurent, D. First evidence of palytoxin and 42-hydroxy-palytoxin in the marine cyanobacterium Trichodesmium. Mar. Drugs 2011, 9, 543–560. [Google Scholar] [CrossRef]
- Ledreux, A.; Krys, S.; Bernard, C. Suitability of the Neuro-2a cell line for the detection of palytoxin and analogues (neurotoxic phycotoxins). Toxicon 2009, 53, 300–308. [Google Scholar] [CrossRef]
- Cañete, E.; Diogène, J. Comparative study of the use of neuroblastoma cells (Neuro-2a) and neuroblastoma-glioma hybrid cells (NG108-15) for the toxic effect quantification of marine toxins. Toxicon 2008, 52, 541–550. [Google Scholar] [CrossRef]
- Rossini, G.P.; Bigiani, A. Palytoxin action on the Na+, K+-ATPase and the disruption of ion equilibria in biological systems. Toxicon 2011, 57, 429–439. [Google Scholar] [CrossRef]
- Bellocci, M.; Sala, G.L.; Prandi, S. The cytolytic and cytotoxic activities of palytoxin. Toxicon 2011, 57, 449–459. [Google Scholar] [CrossRef]
- Espiña, B.; Cagide, E.; Louzao, M.; Fernandez, M.; Vieytes, M.; Katikou, P.; Villar, A.; Jaen, D.; Maman, L.; Botana, L. Specific and dynamic detection of palytoxins by in vitro microplate assay with human neuroblastoma cells. Biosci. Rep. 2009, 29, 13–23. [Google Scholar] [CrossRef]
- Penna, A.; Fraga, S.; Battocchi, C.; Casabianca, S.; Giacobbe, M.G.; Riobo, P.; Vernesi, C. A phylogeographical study of the toxic benthic dinoflagellate genus Ostreopsis Schmidt. J. Biogeogr. 2010, 37, 830–841. [Google Scholar] [CrossRef]
- Pezzolesi, L.; Guerrini, F.; Ciminiello, P.; Dell’Aversano, C.; Iacovo, E.D.; Fattorusso, E.; Forino, M.; Tartaglione, L.; Pistocchi, R. Influence of temperature and salinity on Ostreopsis cf. ovata growth and evaluation of toxin content through HR LC-MS and biological assays. Water Res. 2012, 46, 82–92. [Google Scholar]
- Nascimento, S.M.; Correa, E.V.; Menezes, M.; Varela, D.; Paredes, J.; Morris, S. Growth and toxin profile of Ostreopsis cf. ovata (Dinophyta) from Rio de Janeiro, Brazil. Harmful Algae 2012, 13, 1–9. [Google Scholar]
- Vidyarathna, N.K.; Graneli, E. Influence of temperature on growth, toxicity and carbohydrate production of a Japanese Ostreopsis ovata strain, a toxic-bloom-forming dinoflagellate. Aquat. Microb. Ecol. 2011, 65, 261–270. [Google Scholar]
- Vidyarathna, N.K.; Graneli, E. Physiological responses of Ostreopsis ovata to changes in N and P availability and temperature increase. Harmful Algae 2013, 21–22, 54–63. [Google Scholar]
- Riobo, P.; Paz, B.; Franco, J.M. Analysis of palytoxin-like in Ostreopsis cultures by liquid chromatography with precolumn derivatization and fluorescence detection. Anal. Chim. Acta 2006, 566, 217–223. [Google Scholar] [CrossRef]
- Habermann, E.; Ahnerthilger, G.; Chhatwal, G.S.; Beress, L. Delayed hemolytic action of palytoxin. General characteristics. General characteristics. Biochim. Biophys. Acta 1981, 649, 481–486. [Google Scholar] [CrossRef]
- Habermann, E.; Chhatwal, G.S.; Hessler, H.J. Palytoxin raises the nonspecific permeability of erythrocytes in an ouabain-sensitive manner. Naunyn-Schmiedeberg’s Arch. Pharmacol. 1981, 317, 374. [Google Scholar]
- Habermann, E.; Chhatwal, G.S. Ouabain inhibits the increase due to palytoxin of cation permeability of erythrocytes. Naunyn-Schmiedeberg’s Arch. Pharmacol. 1982, 319, 101–107. [Google Scholar]
- Bignami, G.S. A rapid and sensitive hemolysis neutralization assay for palytoxin. Toxicon 1993, 31, 817–820. [Google Scholar] [CrossRef]
- Taniyama, S.; Arakawa, O.; Terada, M.; Nishio, S.; Takatani, T.; Mahmud, Y.; Noguchi, T. Ostreopsis sp., a possible origin of palytoxin (PTX) in parrotfish Scarus ovifrons. Toxicon 2003, 42, 29–33. [Google Scholar] [CrossRef]
- Riobo, P.; Paz, B.; Franco, J.M.; Vazquez, J.A.; Murado, M.A. Proposal for a simple and sensitive haemolytic assay for palytoxin. Toxicological dynamics, kinetics, ouabain inhibition and thermal stability. Harmful Algae 2008, 7, 415–429. [Google Scholar]
- Taniyama, S.; Mahmud, Y.; Terada, M.; Takatani, T.; Arakawa, O.; Noguchi, T. Occurrence of a food poisoning incident by palytoxin from a serranid Epinephelus sp. in Japan. J. Nat. Toxins 2002, 11, 277–282. [Google Scholar]
- Rhodes, L.; Adamson, J.; Suzuki, T.; Briggs, L.; Garthwaite, I. Toxic marine epiphytic dinoflagellates, Ostreopsis siamensis and Coolia monotis (Dinophyceae), in New Zealand. N. Z. J. Mar. Freshw. Res. 2000, 34, 371–383. [Google Scholar] [CrossRef]
- Rhodes, L.; Towers, N.; Briggs, L.; Munday, R.; Adamson, J. Uptake of palytoxin-like compounds by shellfish fed Ostreopsis siamensis (Dinophyceae). N. Z. J. Mar. Freshw. Res. 2002, 36, 631–636. [Google Scholar] [CrossRef]
- Rhodes, L.L.; Smith, K.F.; Munday, R.; Selwood, A.I.; McNabb, P.S.; Holland, P.T.; Bottein, M.-Y. Toxic dinoflagellates (Dinophyceae) from Rarotonga, Cook Islands. Toxicon 2010, 56, 751–758. [Google Scholar] [CrossRef]
- Gleibs, S.; Mebs, D.; Werding, B. Studies on the origin and distribution of palytoxin in a Caribbean coral reef. Toxicon 1995, 33, 1531–1537. [Google Scholar] [CrossRef]
- Gleibs, S.; Mebs, D. Distribution and sequestration of palytoxin in coral reef animals. Toxicon 1999, 37, 1521–1527. [Google Scholar] [CrossRef]
- Wachi, K.M.; Hokama, Y.; Haga, L.S.; Shiraki, A.; Takenaka, W.E.; Bignami, G.S.; Levine, L. Evidence for palytoxin as one of the sheep erythrocyte lytic in lytic factors in crude extracts of ciguateric and non-ciguateric reef fish tissue. J. Nat. Toxins 2000, 9, 139–146. [Google Scholar]
- Wachi, K.M.; Hokama, Y. Diversity of marine biotoxins in the near-shore ocean area: Presence of a palytoxin-like entity at Barber’s Point Harbor, Oahu. J. Nat. Toxins 2001, 10, 317–333. [Google Scholar]
- Seemann, P.; Gernert, C.; Schmitt, S.; Mebs, D.; Hentschel, U. Detection of hemolytic bacteria from Palythoa caribaeorum (Cnidaria, Zoantharia) using a novel palytoxin-screening assay. Antonie Leeuwenhoek Int. J. Gen. Mol. Microbiol. 2009, 96, 405–411. [Google Scholar] [CrossRef]
- Huang, K.M.; Liu, S.M.; Huang, Y.W.; Huang, K.L.; Hwang, D.F. Food poisoning caused by Sunfish Masturus lanceolatus in Taiwan. J. Food Drug Anal. 2011, 19, 191–196. [Google Scholar]
- Bire, R.; Trotereau, S.; Lemee, R.; Delpont, C.; Chabot, B.; Aumond, Y.; Krys, S. Occurrence of palytoxins in marine organisms from different trophic levels of the French Mediterranean coast harvested in 2009. Harmful Algae 2013, 28, 10–22. [Google Scholar] [CrossRef]
- Brissard, C.; Herrenknecht, C.; Sechet, V.; Herve, F.; Pisapia, F.; Harcouet, J.; Lemee, R.; Chomerat, N.; Hess, P.; Amzil, Z. Complex toxin profile of French Mediterranean Ostreopsis cf. ovata strains, seafood accumulation and ovatoxins prepurification. Mar. Drugs 2014, 12, 2851–2876. [Google Scholar]
- Pagliara, P.; Caroppo, C. Cytotoxic and antimitotic activities in aqueous extracts of eight cyanobacterial strains isolated from the marine sponge Petrosia ficiformis. Toxicon 2011, 57, 889–896. [Google Scholar] [CrossRef]
- Igarashi, T.; Aritake, S.; Yasumoto, T. Mechanisms underlying the hemolytic and ichthyotoxic activities of maitotoxin. Nat. Toxins 1999, 7, 71–79. [Google Scholar] [CrossRef]
- Volpe, G.; Cozzi, L.; Migliorelli, D.; Croci, L.; Palleschi, G. Development of a haemolytic-enzymatic assay with mediated amperometric detection for palytoxin analysis: Application to mussels. Anal. Bioanal. Chem. 2014, 406, 2399–2410. [Google Scholar] [CrossRef]
- Alfonso, A.; Fernandez-Araujo, A.; Alfonso, C.; Carames, B.; Tobio, A.; Louzao, M.C.; Vieytes, M.R.; Botana, L.M. Palytoxin detection and quantification using the fluorescence polarization technique. Anal. Biochem. 2012, 424, 64–70. [Google Scholar] [CrossRef]
- Vale-Gonzalez, C.; Pazos, M.; Alfonso, A.; Vieytes, M.; Botana, L. Study of the neuronal effects of ouabain and palytoxin and their binding to Na+, K+-ATPases using an optical biosensor. Toxicon 2007, 50, 541–552. [Google Scholar] [CrossRef]
- Alfonso, A.; Pazos, M.-J.; Fernández-Araujo, A.; Tobio, A.; Alfonso, C.; Vieytes, M.R.; Botana, L.M. Surface plasmon resonance biosensor method for palytoxin detection based on Na+, K+-ATPase affinity. Toxins 2014, 6, 96–107. [Google Scholar]
- Scheuer, P.; Takahaski, W.; Tsutsumi, J.; Yoshida, T. Ciguatoxin: Isolation and chemical structure. Science 1967, 155, 1267–1268. [Google Scholar] [CrossRef]
- Lange, W.R. Ciguatera fish poisoning. Am. Fam. Phys. 1994, 50, 579–584. [Google Scholar]
- Yasumoto, T.; Nakajima, I.; Bagnis, R.; Adachi, R. Finding of a dinoflagellate as a likely culprit of ciguatera. Bull. Jpn. Soc. Sci. Fish. 1977, 43, 1021–1026. [Google Scholar] [CrossRef]
- Bagnis, R.; Hurtel, J.M.; Chanteau, S.; Chungue, E.; Inoue, A.; Yasumoto, T. Dinoflagellate Gambierdiscus—Toxicus Adachi et Fukuyo—Probable cause of ciguatera. Comptes Rendus Hebd. Seances L’Acad. Sci. D 1979, 289, 671–674. [Google Scholar]
- Bagnis, R.; Chanteau, S.; Chungue, E.; Hurtel, J.M.; Yasumoto, T.; Inoue, A. Origins of ciguatera fish poisoning—A new Dinoflagellate, Gambierdiscus-Toxicus Adachi and Fukuyo, definitively involved as a causal agent. Toxicon 1980, 19, 199–208. [Google Scholar] [CrossRef]
- Sperr, A.E.; Doucette, G.J. Variation in growth rate and ciguatera toxin production among geographically distinct isolates of Gambierdiscus toxicus. In Harmful and Toxic Algal Blooms; Yasumoto, T., Oshima, Y., Fukuyo, Y., Eds.; IOC of UNESCO: Paris, France, 1996; pp. 309–312. [Google Scholar]
- Litaker, R.W.; Vandersea, M.W.; Faust, M.A.; Kibler, S.R.; Nau, A.W.; Holland, W.C.; Chinain, M.; Holmes, M.J.; Tester, P.A. Global distribution of ciguatera causing dinoflagellates in the genus Gambierdiscus. Toxicon 2010, 56, 711–730. [Google Scholar] [CrossRef]
- Chinain, M.; Darius, H.T.; Ung, A.; Cruchet, P.; Wang, Z.; Ponton, D.; Laurent, D.; Pauillac, S. Growth and toxin production in the ciguatera-causing dinoflagellate Gambierdiscus polynesiensis (Dinophyceae) in culture. Toxicon 2010, 56, 739–750. [Google Scholar] [CrossRef]
- Mills, A.R. Poisonous fish in the South Pacific. J. Trop. Med. Hyg. 1956, 59, 99–103. [Google Scholar]
- Vernoux, J.P.; Lahlou, N.; Elandaloussi, S.A.; Riyeche, N.; Magras, L.P. A study of the distribution of ciguatoxin in indiviual Caribbean fish. Acta Trop. 1985, 42, 225–233. [Google Scholar]
- Murata, M.; Legrand, A.M.; Ishibashi, Y.; Yasumoto, T. Structures of ciguatoxin and its congener. J. Am. Chem. Soc. 1989, 111, 8929–8931. [Google Scholar] [CrossRef]
- Murata, M.; Legrand, A.N.; Ishibashi, Y.; Fukui, M.; Yasumoto, T. Conformations of ciguatoxin and related polyethers. Abstr. Pap. Am. Chem. Soc. 1990, 200. 54-AGFD. [Google Scholar]
- Lewis, R.J.; Sellin, M.; Poli, M.A.; Norton, R.S.; MacLeod, J.K.; Sheil, M.M. Purification and characterization of ciguatoxins from moray eel (Lycodontis javanicus, Muraenidae). Toxicon 1991, 29, 1115–1127. [Google Scholar] [CrossRef]
- Satake, M.; Ishimaru, T.; Legrand, A.M.; Yasumoto, T. Isolation of a ciguatoxin analog from cultures of Gambierdiscus toxicus. In Toxic Pyhtoplankton Blooms in the Sea; Samyda, T.J., Shimizu, Y., Eds.; Elsevier: New York, NY, USA, 1993; Volume 3; pp. 575–579. [Google Scholar]
- Satake, M.; Murata, M.; Yasumoto, T. The structure of CTX3C, a ciguatoxin congener isolated from cultured Gambierdiscus toxicus. Tetrahedron Lett. 1993, 34, 1975–1978. [Google Scholar] [CrossRef]
- Satake, M.; Morohashi, A.; Oguri, H.; Oishi, T.; Hirama, M.; Harada, N.; Yasumoto, T. The absolute configuration of ciguatoxin. J. Am. Chem. Soc. 1997, 119, 11325–11326. [Google Scholar] [CrossRef]
- Satake, M.; Fukui, M.; Legrand, A.M.; Cruchet, P.; Yasumoto, T. Isolation and structures of new ciguatoxin analogs, 2,3-dihydroxyCTX3C and 51-hydroxyCTX3C, accumulated in tropical reef fish. Tetrahedron Lett. 1998, 39, 1197–1198. [Google Scholar] [CrossRef]
- Lehane, L.; Lewis, R.J. Ciguatera: Recent advances but the risk remains. Int. J. Food Microbiol. 2000, 61, 91–125. [Google Scholar] [CrossRef]
- Lewis, R.J.; Vernoux, J.P.; Brereton, I.M. Structure of Caribbean ciguatoxin isolated from Caranx latus. J. Am. Chem. Soc. 1998, 120, 5914–5920. [Google Scholar] [CrossRef]
- Hamilton, B.; Hurbungs, M.; Vernoux, J.P.; Jones, A.; Lewis, R.J. Isolation and characterisation of Indian Ocean ciguatoxin. Toxicon 2002, 40, 685–693. [Google Scholar] [CrossRef]
- Bidard, J.N.; Vijverberg, H.P.M.; Frelin, C.; Chungue, E.; Legrand, A.M.; Bagnis, R.; Lazdunski, M. Ciguatoxin is a novel type of Na+ channel toxin. J. Biol. Chem. 1984, 259, 8353–8357. [Google Scholar]
- Molgo, J.; Comella, J.X.; Legrand, A.M. Ciguatoxin enhances quantal transmitter release from frog motor-nerve terminals. Br. J. Pharmacol. 1990, 99, 695–700. [Google Scholar] [CrossRef]
- Molgo, J.; Gaudrytalarmain, Y.M.; Legrand, A.M.; Moulian, N. Ciguatoxin extracted from poisonous moray eels gymnothorax-javanicus triggers acetylcholine-release from torpedo cholinergic synaptosomes via reversed Na+/Ca2+ exchange. Neurosci. Lett. 1993, 160, 65–68. [Google Scholar] [CrossRef]
- Benoit, E.; Juzans, P.; Legrand, A.M.; Molgo, J. Nodal swelling produced by ciguatoxin-induced selective activation of sodium channels in myelinated nerve fibers. Neuroscience 1996, 71, 1121–1131. [Google Scholar] [CrossRef] [PubMed]
- Mattei, C.; Dechraoui, M.Y.; Molgo, J.; Meunier, F.A.; Legrand, A.M.; Benoit, E. Neurotoxins targetting receptor site 5 of voltage-dependent sodium channels increase the nodal volume of myelinated axons. J. Neurosci. Res. 1999, 55, 666–673. [Google Scholar] [CrossRef] [PubMed]
- Lewis, R.J. Ion channel toxins and therapeutics: From cone snail venoms to ciguatera. Ther. Drug Monit. 2000, 22, 61–64. [Google Scholar] [CrossRef]
- Caillaud, A.; de la Iglesia, P.; Darius, H.T.; Pauillac, S.; Aligizaki, K.; Fraga, S.; Chinain, M.; Diogene, J. Update on methodologies available for ciguatoxin determination: Perspectives to confront the onset of ciguatera fish poisoning in Europe. Mar. Drugs 2010, 8, 1838–1907. [Google Scholar] [CrossRef]
- Perez-Arellano, J.L.; Luzardo, O.P.; Brito, A.P.; Cabrera, M.H.; Zumbado, M.; Carranza, C.; Angel-Moreno, A.; Dickey, R.W.; Boada, L.D. Ciguatera fish poisoning, Canary Islands. Emerg. Infect. Dis. 2005, 11, 1981–1982. [Google Scholar] [CrossRef]
- Gouveia, N.; Delgado, J.; Vale, P. Primeiro registo da ocorrência de episódios do tipo ciguatérico no arquipélago da Madeira. In Abstract Book of X Reuniao Oberica, Fitoplancton Toxico e Biotoxinas; IPIMAR: Lisbon, Portugal, 2009. [Google Scholar]
- Fraga, S. Global climate change and harmful algal blooms (HABs). In Abstract Book of 4th European Phycological Congress; Elsevier Science: Oviedo, Spain, 2007; p. 41. [Google Scholar]
- Stebbing, A.R.D.; Turk, S.M.T.; Wheeler, A.; Clarke, K.R. Immigration of southern fish species to south-west England linked to warming of the North Atlantic (1960–2001). J. Mar. Biol. Assoc. UK 2002, 82, 177–180. [Google Scholar] [CrossRef]
- CDC (Centers for Disease Control and Prevention). Cluster of Ciguatera Fish Poisoning,2007. Morbidity and Mortality Weekly Report (MMWR); CDC: North Carolina, NC, USA, 2009; pp. 283–285. [Google Scholar]
- Hokama, Y.; Banner, A.H.; Boylan, D.B. A radioimmunoassay for the detection of ciguatoxin. Toxicon 1977, 15, 317–325. [Google Scholar] [CrossRef]
- Hokama, Y.; Abad, M.A.; Kimura, L.H. A rapid enzyme-immunoassay for the detection of ciguatoxin in contaminated fish-tissues. Toxicon 1983, 21, 817–824. [Google Scholar] [CrossRef]
- Hokama, Y. A rapid, simplified enzyme-immunoassay stick test for the detecction of ciguatoxin and related polyethers from fish-tissues. Toxicon 1985, 23, 939–946. [Google Scholar] [CrossRef]
- Hokama, Y.; Shirai, L.K.; Iwamoto, L.M.; Kobayashi, M.N.; Goto, C.S.; Nakagawa, L.K. Assessment of a rapid enzyme-immunoassay stick test for the detection of ciguatoxin and related polyether toxins in fish-tissues. Biol. Bull. 1987, 172, 144–153. [Google Scholar] [CrossRef]
- Park, D.L. Detection of ciguatera and diarrheic shellfish toxins in finfish and shellfish with ciguatect kit. J. AOAC Int. 1995, 78, 533–537. [Google Scholar]
- Bienfang, P.; DeFelice, S.; Dowling, A. Quantitative evaluation of commercially available test kit for ciguatera in fish. Food Nutr. Sci. 2011, 2, 594–598. [Google Scholar] [CrossRef]
- Ebesu, J.S.; Campora, C.E. Comment on “Quantitative evaluation of commercially available test kit for ciguatera in fish”. Food Nutr. Sci. 2012, 3, 1233–1237. [Google Scholar] [CrossRef]
- Dickey, R.W.; Granade, H.R.; McClure, F.D. uatect Solid-Phase Immunobead Assay for the Detection of Ciguatera-Related Biotoxins in Caribbean Finfish. In Proceedings of the International Workshop on Ciguatera Management, Brisbane, Australia, 13–16 April 1994; Memoirs of the Queensland Museum: Brisbane, Australia, 1994; pp. 481–488. [Google Scholar]
- Hokama, Y.; Honda, S.A.A.; Kobayashi, M.N.; Nakagawa, L.K.; Asahina, A.Y.; Miyahara, J.T. Monoclonal-antibody (MAb) in detection of ciguatoxin (CTX) and related polyethers by the stick-enzyme immunoassay (S-EIA) in fish-tissues associated with ciguatera poisoning. In Mycotoxins and Phycotoxins; Natori, S., Hashimoto, K., Ueno, Y., Eds.; Elsevier: Amsterdam, The Netherlands, 1989; Volume 10; pp. 303–310. [Google Scholar]
- Campora, C.E.; Hokama, Y.; Ebesu, J.S.M. Comparative analysis of purified pacific and Caribbean ciguatoxin congeners and related marine toxins using a modified ELISA technique. J. Clin. Lab. Anal. 2006, 20, 121–125. [Google Scholar] [CrossRef]
- Pauillac, S.; Sasaki, M.; Inoue, M.; Naar, J.; Branaa, P.; Chinain, M.; Tachibana, K.; Legrand, A.M. Characterization of mice antisera elicited with a ciguatoxin tetracyclic synthetic ring fragment (JKLM) conjugated to carrier proteins. Toxicon 2000, 38, 669–685. [Google Scholar] [CrossRef]
- Campora, C.E.; Dierking, J.; Tamaru, C.S.; Hokama, Y.; Vincent, D. Detection of ciguatoxin in fish tissue using sandwich ELISA and neuroblastoma cell bioassay. J. Clin. Lab. Anal. 2008, 22, 246–253. [Google Scholar] [CrossRef]
- Campora, C.E.; Hokama, Y.; Yabusaki, K.; Isobe, M. Development of an enzyme-linked immunosorbent assay for the detection of ciguatoxin in fish tissue using chicken immunoglobulin Y. J. Clin. Lab. Anal. 2008, 22, 239–245. [Google Scholar] [CrossRef]
- Oguri, H.; Hirama, M.; Tsumuraya, T.; Fujii, I.; Maruyama, M.; Uehara, H.; Nagumo, Y. Synthesis-based approach toward direct sandwich immunoassay for ciguatoxin CTX3C. J. Am. Chem. Soc. 2003, 125, 7608–7612. [Google Scholar] [CrossRef]
- Tsumuraya, T.; Fujii, I.; Inoue, M.; Tatami, A.; Miyazaki, K.; Hirama, M. Production of monoclonal antibodies for sandwich immunoassay detection of ciguatoxin 51-hydroxyCTX3C. Toxicon 2006, 48, 287–294. [Google Scholar] [CrossRef]
- Tsumuraya, T.; Fujii, I.; Hirama, M. Production of monoclonal antibodies for sandwich immunoassay detection of Pacific ciguatoxins. Toxicon 2010, 56, 797–803. [Google Scholar] [CrossRef]
- Tsumuraya, T.; Takeuchi, K.; Yamashita, S.; Fujii, I.; Hirama, M. Development of a monoclonal antibody against the left wing of ciguatoxin CTX1B: Thiol strategy and detection using a sandwich ELISA. Toxicon 2012, 60, 348–357. [Google Scholar] [CrossRef]
- Tsumuraya, T.; Fujii, I.; Hirama, M. Preparation of anti-ciguatoxin monoclonal antibodies using synthetic haptens: Sandwich ELISA detection of ciguatoxins. J. AOAC Int. 2014, 97, 373–379. [Google Scholar] [CrossRef]
- Manger, R.L.; Leja, L.S.; Lee, S.Y.; Hungerford, J.M.; Wekell, M.M. Tetrazolium-based cell bioassay for neurotoxins active on voltage-sensitive sodium channels: Semiautomated assay for saxitoxins, brevetoxins, and ciguatoxins. Anaytical Biochem. 1993, 214, 190–194. [Google Scholar] [CrossRef]
- Cañete, E.; Diogène, J. Improvements in the use of neuroblastomaxglioma hybrid cells (NG108-15) for the toxic effect quantification of marine toxins. Toxicon 2010, 55, 381–389. [Google Scholar] [CrossRef]
- Fairey, E.R.; Edmunds, J.; Deamer-Melia, N.J.; Glasgow, H., Jr.; Johnson, F.M.; Moeller, P.R.; Burkholder, J.; Ramsdell, J.S. Reporter gene assay for fish-killing activity produced by Pfiesteria piscicida. Environ. Health Perspect. 1999, 107, 711–714. [Google Scholar] [CrossRef]
- Landsberg, J.; Flewelling, L.; Naar, J. Karenia brevis red tides, brevetoxins in the food web, and impacts on natural resources: Decadal advancements. Harmful Algae 2009, 8, 598–607. [Google Scholar] [CrossRef]
- McFarren, E.; Tanabe, H.; Silva, F.; Wilson, W.; Campbell, J.; Lewis, K. The occurrence of a ciguatera-like poison in oysters, clams, and Gymnodinium breve cultures. Toxicon 1965, 3, 111–123. [Google Scholar] [CrossRef]
- Watkins, S.M.; Reich, A.; Fleming, L.E.; Hammond, R. Neurotoxic shellfish poisoning. Mar. Drugs 2008, 6, 431–455. [Google Scholar] [CrossRef]
- Woofter, R.T.; Brendtro, K.; Ramsdell, J.S. Uptake and elimination of brevetoxin in blood of striped mullet (Mugil cephalus) after aqueous exposure to Karenia brevis. Environ. Health Perspect. 2005, 113, 11–16. [Google Scholar] [CrossRef] [PubMed]
- Dechraoui, M.; Bottein, Y.; Tiedeken, J.A.; Persad, R.; Wang, Z.; Granade, H.; Dickey, R.W.; Ramsdell, J.S. Use of two detection methods to discriminate ciguatoxins from brevetoxins: Application to great barracuda from Florida Keys. Toxicon 2005, 46, 261–270. [Google Scholar] [CrossRef]
- Bottein Dechraoui, M.-Y.; Wacksman, J.J.; Ramsdell, J.S. Species selective resistance of cardiac muscle voltage gated sodium channels: Characterization of brevetoxin and ciguatoxin binding sites in rats and fish. Toxicon 2006, 48, 702–712. [Google Scholar] [CrossRef]
- Manger, R.; Woodle, D.; Berger, A.; Dickey, R.W.; Jester, E.; Yasumoto, T.; Lewis, R.; Hawryluk, T.; Hungerford, J. Flow cytometric-membrane potential detection of sodium channel active marine toxins: Application to ciguatoxins in fish muscle and feasibility of automating saxitoxin detection. J. AOAC Int. 2014, 97, 299–306. [Google Scholar] [CrossRef]
- Lewis, R.J. Detection of toxins associated with ciguatera fish poisoning. In Manual on Harmful Marine Microalgae; Hallegraeff, G.M., Anderson, D.M., Cembella, A.D., Eds.; UNESCO: Paris, France, 2003; p. 267. [Google Scholar]
- Yasumoto, T.; Fukui, M.; Sasaki, K.; Sugiyama, K. Determinations of marine toxins in foods. J. AOAC Int. 1995, 78, 574–582. [Google Scholar]
- Campora, C.E.; Hokama, Y.; Tamaru, C.S.; Anderson, B.; Vincent, D. Evaluating the risk of ciguatera fish poisoning from reef fish grown at marine aquaculture facilities in Hawaii. J. World Aquac. Soc. 2010, 41, 61–70. [Google Scholar] [CrossRef]
- Dickey, R.W.; Granade, H.R.; Jester, E.L.E.; Abraham, A.; El Said, K.R.; Plakas, S.M. termination of Caribbean and Pacific ciguatoxins in fish and formulation of regulatory advisory levels. In Proceedings of the Ciguatera and Related Biotoxins Workshop, Noumea, New Caledonia, 27–31 October 2008.
- Darius, H.T.; Ponton, D.; Revel, T.; Cruchet, P.; Ung, A.; Fouc, M.T.; Chinain, M. Ciguatera risk assessment in two toxic sites of French Polynesia using the receptor-binding assay. Toxicon 2007, 50, 612–626. [Google Scholar] [CrossRef]
- Wu, J.J.; Mak, Y.L.; Murphy, M.B.; Lam, J.C.W.; Chan, W.H.; Wang, M.; Chan, L.L.; Lam, P.K.S. Validation of an accelerated solvent extraction liquid chromatography-tandem mass spectrometry method for Pacific ciguatoxin-1 in fish flesh and comparison with the mouse neuroblastoma assay. Anal. Bioanal. Chem. 2011, 400, 3165–3175. [Google Scholar] [CrossRef]
- Bottein Dechraoui, M.Y.; Wang, Z.; Turquet, J.; Chinain, M.; Darius, T.; Cruchet, P.; Radwan, F.F.; Dickey, R.W.; Ramsdell, J.S. Biomonitoring of ciguatoxin exposure in mice using blood collection cards. Toxicon 2005, 46, 243–251. [Google Scholar] [CrossRef]
- Bottein Dechraoui, M.Y.; Wang, Z.; Ramsdell, J.S. Optimization of ciguatoxin extraction method from blood for Pacific ciguatoxin (P-CTX-1). Toxicon 2007, 49, 100–105. [Google Scholar] [CrossRef]
- Ledreux, A.; Ramsdell, J.S. Bioavailability and intravenous toxicokinetic parameters for Pacific ciguatoxin P-CTX-1 in rats. Toxicon 2013, 64, 81–86. [Google Scholar] [CrossRef] [PubMed]
- Bottein Dechraoui, M.Y.; Wang, Z.; Ramsdell, J.S. Toxicokinetics of the ciguatoxin P-CTX-1 in rats after intraperitoneal or oral administration. Toxicology 2011, 284, 1–6. [Google Scholar] [CrossRef]
- O’Toole, A.C.; Dechraoui Bottein, M.-Y.; Danylchuk, A.J.; Ramsdell, J.S.; Cooke, S.J. Linking ciguatera poisoning to spatial ecology of fish: A novel approach to examining the distribution of biotoxin levels in the great barracuda by combining non-lethal blood sampling and biotelemetry. Sci. Total Environ. 2012, 427, 98–105. [Google Scholar] [CrossRef]
- Fraga, S.; Rodríguez, F.; Caillaud, A.; Diogène, J.; Raho, N.; Zapata, M. Gambierdiscus excentricus sp. nov. (Dinophyceae), a benthic toxic dinoflagellate from the Canary Islands (NE Atlantic Ocean). Harmful Algae 2011, 11, 10–22. [Google Scholar]
- Caillaud, A.; de la Iglesia, P.; Barber, E.; Eixarch, H.; Mohammad-Noor, N.; Yasumoto, T.; Diogene, J. Monitoring of dissolved ciguatoxin and maitotoxin using solid-phase adsorption toxin tracking devices: Application to Gambierdiscus pacificus in culture. Harmful Algae 2011, 10, 433–446. [Google Scholar] [CrossRef]
- Shimojo, R.Y.; Iwaoka, W.T. A rapid hemolysis assay for the detection of sodium channel-specific marine toxins. Toxicology 2000, 154, 1–7. [Google Scholar] [CrossRef]
- Holland, W.C.; Litaker, R.W.; Tomas, C.R.; Kibler, S.R.; Place, A.R.; Davenport, E.D.; Tester, P.A. Differences in the toxicity of six Gambierdiscus (Dinophyceae) species measured using an in vitro human erythrocyte lysis assay. Toxicon 2013, 65, 15–33. [Google Scholar] [CrossRef]
- Poli, M.A.; Lewis, R.J.; Dickey, R.W.; Musser, S.M.; Buckner, C.A.; Carpenter, L.G. Identification of Caribbean ciguatoxins as the cause of an outbreak of fish poisoning among US soldiers in Haiti. Toxicon 1997, 35, 733–741. [Google Scholar] [CrossRef]
- Yasumoto, T. Search for ciguatoxin producing clones of Gambierdiscus toxicus on Okinawa coastal reefs. In Abstract book of the Ciguatera and related biotoxins workshop, 27–31 October 2008; Institut de recherche pour le développement (IRD): Noumea, New Caledonia.
- McCall, J.R.; Jacocks, H.M.; Niven, S.C.; Poli, M.A.; Baden, D.G.; Bourdelais, A.J. Development and utilization of a fluorescence-based receptor-binding assay for the site 5 voltage-sensitive sodium channel ligands brevetoxin and ciguatoxin. J. AOAC Int. 2014, 97, 307–315. [Google Scholar] [CrossRef]
- Caillaud, A.; Eixarch, H.; de la Iglesia, P.; Rodriguez, M.; Dominguez, L.; Andree, K.; Diogene, J. Towards the standardisation of the neuroblastoma (neuro-2a) cell-based assay for ciguatoxin-like toxicity detection in fish: Application to fish caught in the Canary Islands. Food Add. Contam. A 2012, 29, 1000–1001. [Google Scholar] [CrossRef]
- Robertson, A.; Garcia, A.C.; Quintana, H.A.F.; Smith, T.B.; Castillo, B.F.; Reale-Munroe, K.; Gulli, J.A.; Olsen, D.A.; Hooe-Rollman, J.I.; Jester, E.L.E.; et al. Invasive lionfish (Pterois volitans): A potential human health threat for ciguatera fish poisoning in tropical waters. Mar. Drugs 2014, 12, 88–97. [Google Scholar]
- Kerbrat, A.-S.; Darius, H.T.; Pauillac, S.; Chinain, M.; Laurent, D. Detection of ciguatoxin-like and paralysing toxins in Trichodesmium spp. from New Caledonia lagoon. from New Caledonia lagoon. Mar. Pollut. Bull. 2010, 61, 360–366. [Google Scholar] [CrossRef]
- Otero, A.; Chapela, M.-J.; Atanassova, M.; Vieites, J.M.; Cabado, A.G. Cyclic Imines: Chemistry and mechanism of action: A review. Chem. Res. Toxicol. 2011, 24, 1817–1829. [Google Scholar] [CrossRef]
- Chatzianastasiou, M.; Katikou, P.; Zacharaki, T.; Papazachariou, A.; McKevitt, A. Cyclic imines, as emerging marine toxins: Chemical properties, distribution, toxicological aspects and detection methods. J. Hell. Vet. Med. Soc. 2011, 62, 240–248. [Google Scholar]
- Cembella, A.; Lewis, N.; Quilliam, M. The marine dinoflagellate Alexandrium ostenfeldii (Dinophyceae) as the causative organism of spirolide shellfish toxins. Phycologia 2000, 39, 67–74. [Google Scholar] [CrossRef]
- Touzet, N.; Franco, J.M.; Raine, R. Morphogenetic diversity and biotoxin composition of Alexandrium (Dinophyceae) in Irish coastal waters. Harmful Algae 2008, 7, 782–797. [Google Scholar] [CrossRef]
- Seki, T.; Satake, M.; Mackenzie, L.; Kaspar, H.F.; Yasumoto, T. Gymnodimine, a new marine toxin of unprecedented structure isolated from New-Zeland oysters and the dinofalgellate Gymnnodinium sp. Tetrahedron Lett. 1995, 36, 7093–7096. [Google Scholar] [CrossRef]
- Rhodes, L.; Smith, K.; Selwood, A.; McNabb, P.; van Ginkel, R.; Holland, P.; Munday, R. Production of pinnatoxins by a peridinoid dinoflagellate isolated from Northland, New Zealand. Harmful Algae 2010, 9, 384–389. [Google Scholar] [CrossRef]
- Nezan, E.; Chomerat, N. Vulcanodinium rugosum gen. nov., sp. nov. (Dinophyceae): A new marine dinoflagellate from the French Mediterranean coast. Cryptogam. Algol. 2011, 32, 3–18. [Google Scholar]
- Torigoe, K.; Murata, M.; Yasumoto, T.; Iwashita, T. Prorocentrolide, a toxic nitrogenous macrocycle from a marine dinoflagellate, Prorocentrum lima. J. Am. Chem. Soc. 1988, 110, 7876–7877. [Google Scholar] [CrossRef]
- Lu, C.K.; Lee, G.H.; Huang, R.; Chou, H.N. Spiro-prorocentrimine, a novel macrocyclic lactone from a benthic Prorocentrum sp. of Taiwan. Tetrahedron Lett. 2001, 42, 1713–1716. [Google Scholar] [CrossRef]
- Miles, C.O.; Wilkins, A.L.; Stirling, D.J.; MacKenzie, A.L. Gymnodimine C, an isomer of gymnodimine B, from Karenia selliformis. J. Agric. Food Chem. 2003, 51, 4838–4840. [Google Scholar] [CrossRef]
- Van Wagoner, R.M.; Misner, I.; Tomas, C.R.; Wright, J.L. Occurrence of 12-methylgymnodimine in a spirolide-producing dinoflagellate Alexandrium peruvianum and the biogenetic implications. Tetrahedron Lett. 2011, 52, 4243–4246. [Google Scholar] [CrossRef]
- Gueret, S.M.; Brimble, M.A. Spiroimine shellfish poisoning (SSP) and the spirolide family of shellfish toxins: Isolation, structure, biological activity and synthesis. Nat. Prod. Rep. 2010, 27, 1350–1366. [Google Scholar] [CrossRef]
- Selwood, A.I.; Miles, C.O.; Wilkins, A.L.; van Ginkel, R.; Munday, R.; Rise, F.; McNabb, P. Isolation, structural determination and acute toxicity of pinnatoxins E, F and G. J. Agric. Food Chem. 2010, 58, 6532–6542. [Google Scholar] [CrossRef]
- Molgó, J.; Girard, E.; Benoit, E. 18 Cyclic Imines: An insight into this emerging group of bioactive marine toxins. In Phycotoxins: Chemistry and Biochemistry; Botana, L.M., Ed.; Wiley-Blackwell: Ames, IA, USA, 2008; pp. 319–335. [Google Scholar]
- Aasen, J.A.; Hardstaff, W.; Aune, T.; Quilliam, M.A. Discovery of fatty acid ester metabolites of spirolide toxins in mussels from Norway using liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom. 2006, 20, 1531–1537. [Google Scholar] [CrossRef]
- McCarron, P.; Rourke, W.A.; Hardstaff, W.; Pooley, B.; Quilliam, M.A. Identification of pinnatoxins and discovery of their fatty acid ester metabolites in mussels (Mytilus edulis) from Eastern Canada. J. Agric. Food Chem. 2012, 60, 1437–1446. [Google Scholar] [CrossRef]
- Hu, T.; deFreitas, A.S.; Curtis, J.M.; Oshima, Y.; Walter, J.A.; Wright, J.L. Isolation and structure of prorocentrolide B, a fast-acting toxin from Prorocentrum maculosum. J. Nat. Prod. 1996, 59, 1010–1014. [Google Scholar] [CrossRef]
- MacKenzie, L.A.; Haywood, A.J.; Adamson, J.; Truman, P.; Till, D.; Seki, T.; Satake, M.; Yasumoto, T. Gymnodimine contamination in shellfish in New Zealand. In Harmful and Toxic Algal Blooms; Yasumoto, T., Oshima, Y., Fukuyo, Y., Eds.; The Intergovernmental Oceanographic Commission of UNESCO: Paris, France, 1996; pp. 97–100. [Google Scholar]
- Uemura, D.; Chou, T.; Haino, T.; Nagatsu, A.; Fukuzawa, S.; Zheng, S.Z.; Chen, H.S. Pinnatoxin A: A toxic amphoteric macrocycle from the okinawan bivalve Pinna muricata. J. Am. Chem. Soc. 1995, 117, 1155–1156. [Google Scholar]
- Bourne, Y.; Radic, Z.; Aráoz, R.; Talley, T.T.; Benoit, E.; Servent, D.; Taylor, P.; Molgó, J.; Marchot, P. Structural determinants in phycotoxins and AChBP conferring high affinity binding and nicotinic AChR antagonism. Proc. Natl. Acad. Sci. USA 2010, 107, 6076–6081. [Google Scholar] [CrossRef]
- Stivala, C.E.; Benoit, E.; Araoz, R.; Servent, D.; Novikov, A.; Molgo, J.; Zakarian, A. Synthesis and biology of cyclic imine toxins, an emerging class of potent, globally distributed marine toxins. Nat. Prod. Rep. 2014. [Google Scholar] [CrossRef]
- Pigozzi, S.; Bianchi, L.; Boschetti, L.; Cangini, M.; Ceredi, A.; Magnani, F.; Milandri, A.; Montanari, S.; Pompei, M.; Riccardi, E. First evidence of spirolide accumulation in northwestern Adriatic shellfish. In Proceedings of the 12th ICHA, 4–8 September 2006; ISSHA and IOC of UNESCO: Copenhagen, Denmark, 2008; pp. 319–322. [Google Scholar]
- Kharrat, R.; Servent, D.; Girard, E.; Ouanounou, G.; Amar, M.; Marrouchi, R.; Benoit, E.; Molgo, J. The marine phycotoxin gymnodimine targets muscular and neuronal nicotinic acetylcholine receptor subtypes with high affinity. J. Neurochem. 2008, 107, 952–963. [Google Scholar]
- Dragunow, M.; Trzoss, M.; Brimble, M.A.; Cameron, R.; Beuzenberg, V.; Holland, P.; Mountfort, D. Investigations into the cellular actions of the shellfish toxin gymnodimine and analogues. Environ. Toxicol. Pharmacol. 2005, 20, 305–312. [Google Scholar] [CrossRef]
- Geiger, M.; Desanglois, G.; Hogeveen, K.; Fessard, V.; Lepretre, T.; Mondeguer, F.; Guitton, Y.; Herve, F.; Sechet, V.; Grovel, O.; et al. Cytotoxicity, fractionation and dereplication of extracts of the Dinoflagellate Vulcanodinium rugosum, a producer of pinnatoxin G. Mar. Drugs 2013, 11, 3350–3371. [Google Scholar] [CrossRef]
- Hellyer, S.D.; Selwood, A.I.; van Ginkel, R.; Munday, R.; Sheard, P.; Miles, C.O.; Rhodes, L.; Kerr, D.S. In vitro labelling of muscle type nicotinic receptors using a fluorophore-conjugated pinnatoxin F derivative. Toxicon 2014, 87, 17–25. [Google Scholar] [CrossRef]
- Tatters, A.O.; Muhlstein, H.I.; Tomas, C.R. The hemolytic activity of Karenia selliformis and two clones of Karenia brevis throughout a growth cycle. J. Appl. Phycol. 2010, 22, 435–442. [Google Scholar] [CrossRef]
- Eschbach, E.; Scharsack, J.P.; John, U.; Medlin, L.K. Improved erythrocyte lysis assay in microtitre plates for sensitive detection and efficient measurement of haemolytic compounds from ichthyotoxic algae. J. Appl. Toxicol. 2001, 21, 513–519. [Google Scholar] [CrossRef]
- Vilariño, N.; Fonfria, E.S.; Molgo, J.; Araoz, R.; Botana, L.M. Detection of gymnodimine-A and 13-desmethyl C spirolide phycotoxins by fluorescence polarization. Anal. Chem. 2009, 81, 2708–2714. [Google Scholar] [CrossRef]
- Fonfria, E.S.; Vilarino, N.; Molgo, J.; Araoz, R.; Otero, P.; Espina, B.; Carmen Louzao, M.; Alvarez, M.; Botana, L.M. Detection of 13,19-didesmethyl C spirolide by fluorescence polarization using Torpedo electrocyte membranes. Anal. Biochem. 2010, 403, 102–107. [Google Scholar] [CrossRef]
- Fonfria, E.S.; Vilarino, N.; Espina, B.; Carmen Louzao, M.; Alvarez, M.; Molgo, J.; Araoz, R.; Botana, L.M. Feasibility of gymnodimine and 13-desmethyl C spirolide detection by fluorescence polarization using a receptor-based assay in shellfish matrixes. Anal. Chim. Acta 2010, 657, 75–82. [Google Scholar] [CrossRef] [PubMed]
- Otero, P.; Alfonso, A.; Alfonso, C.; Araoz, R.; Molgo, J.; Vieytes, M.R.; Botana, L.M. First direct fluorescence polarization assay for the detection and quantification of spirolides in mussel samples. Anal. Chim. Acta 2011, 701, 200–208. [Google Scholar] [CrossRef]
- Rodriguez, L.P.; Vilarino, N.; Molgo, J.; Araoz, R.; Antelo, A.; Vieytes, M.R.; Botana, L.M. Solid-phase receptor-based assay for the detection of cyclic imines by chemiluminescence, fluorescence, or colorimetry. Anal. Chem. 2011, 83, 5857–5863. [Google Scholar] [CrossRef]
- Rodriguez, L.P.; Vilarino, N.; Molgo, J.; Araoz, R.; Botana, L.M. High-throughput receptor-based assay for the detection of spirolides by chemiluminescence. Toxicon 2013, 75, 35–43. [Google Scholar] [CrossRef]
- Araoz, R.; Ramos, S.; Pelissier, F.; Guerineau, V.; Benoit, E.; Vilarino, N.; Botana, L.M.; Zakarian, A.; Molgo, J. Coupling the Torpedo microplate-receptor binding assay with mass spectrometry to detect cyclic imine neurotoxins. Anal. Chem. 2012, 84, 10445–10453. [Google Scholar] [CrossRef]
- Rodriguez, L.P.; Vilarino, N.; Molgo, J.; Araoz, R.; Carmen Louzao, M.; Taylor, P.; Talley, T.; Botana, L.M. Development of a solid-phase receptor-based assay for the detection of cyclic imines using a microsphere-flow cytometry system. Anal. Chem. 2013, 85, 2340–2347. [Google Scholar] [CrossRef]
- Yotsu-Yamashita, M.; Sugimoto, A.; Takai, A.; Yasumoto, T. Effects of specific modifications of several hydroxyls of tetrodotoxin on its affinity to rat brain membrane. J. Pharmacol. Exp. Ther. 1999, 289, 1688–1696. [Google Scholar]
- Bane, V.; Lehane, M.; Dikshit, M.; O’Riordan, A.; Furey, A. Tetrodotoxin: Chemistry, toxicity, source, distribution and detection. Toxins 2014, 6, 693–755. [Google Scholar] [CrossRef]
- Tahara, Y.; Hirata, Y. Studies on the puffer fish toxin. J. Pharm. Soc. 1909, 29, 587–625. [Google Scholar]
- Yokoo, A. Chemical studies on pufferfish toxin (3)-separation of spheroidine. J. Chem. Soc. Jpn. 1950, 71, 590–592. [Google Scholar]
- Tsuda, K.; Tachikawa, R.; Sakai, K.; Tamura, C.; Amakasu, O.; Kawamura, M.; Ikuma, S. On the structure of tetrodotoxin. Chem. Pharm. Bull. 1964, 12, 642–645. [Google Scholar] [CrossRef]
- Woodward, R.; Gougoutas, J.Z. The structure of tetrodotoxin. Pure Appl. Chem. 1964, 9, 49–74. [Google Scholar]
- Goto, T.; Kishi, Y.; Takahashi, S.; Hirata, Y. Tetrodotoxin. Tetrahedron 1965, 21, 2059–2088. [Google Scholar] [CrossRef]
- Noguchi, T.; Arakawa, O.; Takatani, T. TTX accumulation in pufferfish. Comp. Biochem. Physiol. D 2006, 1, 145–152. [Google Scholar]
- Chau, R.; Kalaitzis, J.A.; Neilan, B.A. On the origins and biosynthesis of tetrodotoxin. Aquat. Toxicol. 2011, 104, 61–72. [Google Scholar] [CrossRef]
- Pratheepa, V.; Vasconcelos, V. Microbial diversity associated with tetrodotoxin production in marine organisms. Environ. Toxicol. Pharmacol. 2013, 36, 1046–1054. [Google Scholar] [CrossRef]
- Rodriguez, P.; Alfonso, A.; Vale, C.; Alfonso, C.; Vale, P.; Tellez, A.; Botana, L.M. First toxicity report of tetrodotoxin and 5,6,11-trideoxyTTX in the trumpet shell Charonia lampas lampas in Europe. Anal. Chem. 2008, 80, 5622–5629. [Google Scholar] [CrossRef]
- Bentur, Y.; Ashkar, J.; Lurie, Y.; Levy, Y.; Azzam, Z.S.; Litmanovich, M.; Golik, M.; Gurevych, B.; Golani, D.; Eisenman, A. Lessepsian migration and tetrodotoxin poisoning due to Lagocephalus sceleratus in the eastern Mediterranean. Toxicon 2008, 52, 964–968. [Google Scholar] [CrossRef]
- Silva, M.; Azevedo, J.; Rodriguez, P.; Alfonso, A.; Botana, L.M.; Vasconcelos, V. New gastropod vectors and tetrodotoxin potential expansion in temperate waters of the Atlantic Ocean. Mar. Drugs 2012, 10, 712–726. [Google Scholar] [CrossRef]
- Kawatsu, K.; Hamano, Y.; Yoda, T.; Terano, Y.; Shibata, T. Rapid and highly sensitive enzyme immunoassay for quantitative determination of tetrodotoxin. Jpn. J. Med. Sci. Biol. 1997, 50, 133–150. [Google Scholar] [CrossRef]
- Zhou, Y.; Li, Y.; Pan, F.; Liu, Z.; Wang, Z. Identification of tetrodotoxin antigens and a monoclonal antibody. Food Chem. 2009, 112, 582–586. [Google Scholar] [CrossRef]
- Raybould, T.J.G.; Bignami, G.S.; Inouye, L.K.; Simpson, S.B.; Byrnes, J.B.; Grothaus, P.G.; Vann, D.C. A monoclonal antibody-based immunoassay for detecting tetrodotoxin in biological samples. J. Clin. Lab. Anal. 1992, 6, 65–72. [Google Scholar] [CrossRef]
- Neagu, D.; Micheli, L.; Palleschi, G. Study of a toxin-alkaline phosphatase conjugate for the development of an immunosensor for tetrodotoxin determination. Anal. Bioanal. Chem. 2006, 385, 1068–1074. [Google Scholar] [CrossRef]
- Tao, J.; Wei, W.; Nan, L.; Lei, L.; Hui, H.; Fen, G.; Jun, L.; Jing, Z.; Rong, J. Development of competitive indirect ELISA for the detection of tetrodotoxin and a survey of the distribution of tetrodotoxin in the tissues of wild puffer fish in the waters of south-east China. Food Add. Contam. 2010, 27, 1589–1597. [Google Scholar] [CrossRef]
- Stokes, A.N.; Williams, B.L.; French, S.S. An improved competitive inhibition enzymatic immunoassay method for tetrodotoxin quantification. Biol. Proced. Online 2012, 14, 1–5. [Google Scholar]
- Wang, R.; Huang, A.; Liu, L.; Xiang, S.; Li, X.; Ling, S.; Wang, L.; Lu, T.; Wang, S. Construction of a single chain variable fragment antibody (scFv) against tetrodotoxin (TTX) and its interaction with TTX. Toxicon 2014, 83, 22–34. [Google Scholar] [CrossRef]
- Kreuzer, M.P.; Pravda, M.; O’Sullivan, C.K.; Guilbault, G.G. Novel electrochemical immunosensors for seafood toxin analysis. Toxicon 2002, 40, 1267–1274. [Google Scholar] [CrossRef]
- Yu, C.-H.; Yu, C.-F.; Tam, S.; Hoi-Fu Yu, P. Rapid screening of tetrodotoxin in urine and plasma of patients with puffer fish poisoning by HPLC with creatinine correction. Food Add. Contam. 2010, 27, 89–96. [Google Scholar] [CrossRef]
- Zhou, Y.; Li, Y.; Lu, S.; Ren, H.; Li, Z.; Zhang, Y.; Pan, F.; Liu, W.; Zhang, J.; Liu, Z. Gold nanoparticle probe-based immunoassay as a new tool for tetrodotoxin detection in puffer fish tissues. Sens. Actuators B 2010, 146, 368–372. [Google Scholar] [CrossRef]
- Thattiyaphong, A.; Unahalekhaka, J.; Mekha, N.; Nispa, W.; Kluengklangdon, P.; Rojanapantip, L. Efficiency of a rapid test for detection of tetrodotoxin in puffer fish. J. Immunoass. Immunochem. 2013, 35, 111–119. [Google Scholar]
- Yakes, B.J.; Deeds, J.; White, K.; DeGrasse, S.L. Evaluation of surface plasmon resonance biosensors for detection of tetrodotoxin in food matrices and comparison to analytical methods. J. Agric. Food Chem. 2011, 59, 839–846. [Google Scholar]
- Taylor, A.D.; Ladd, J.; Etheridge, S.; Deeds, J.; Hall, S.; Jiang, S. Quantitative detection of tetrodotoxin (TTX) by a surface plasmon resonance (SPR) sensor. Sens. Actuators B 2008, 130, 120–128. [Google Scholar] [CrossRef]
- Taylor, A.D.; Vaisocherová, H.; Deeds, J.; DeGrasse, S.; Jiang, S. Tetrodotoxin detection by a surface plasmon resonance sensor in pufferfish matrices and urine. J. Sens. 2011. [Google Scholar] [CrossRef]
- Vaisocherova, H.; Taylor, A.D.; Jiang, S.; Hegnerova, K.; Vala, M.; Homola, J.; Yakes, B.J.; Deeds, J.; DeGrasse, S. Surface plasmon resonance biosensor for determination of tetrodotoxin: Prevalidation study. J. AOAC Int. 2011, 94, 596–604. [Google Scholar]
- Campbell, K.; Barnes, P.; Haughey, S.; Higgins, C.; Kawatsu, K.; Vasconcelos, V.; Elliott, C. Development and single laboratory validation of an optical biosensor assay for tetrodotoxin detection as a tool to combat emerging risks in European seafood. Anal. Bioanal. Chem. 2013, 405, 7753–7763. [Google Scholar] [CrossRef]
- Yakes, B.J.; Kanyuck, K.M.; DeGrasse, S.L. First report of a direct surface plasmon resonance immunosensor for a small molecule seafood toxin. Anal. Chem. 2014, 86, 9251–9255. [Google Scholar] [CrossRef] [PubMed]
- Yakes, B.J.; Etheridge, S.M.; Mulvaney, S.P.; Tamanaha, C.R. Fluidic force discrimination assays: A new technology for tetrodotoxin detection. Mar. Drugs 2010, 8, 565–576. [Google Scholar] [CrossRef]
- Kogure, K.; Tamplin, M.L.; Simidu, U.; Colwell, R.R. A tissue culture assay for tetrodotoxin, saxitoxin and related toxins. Toxicon 1988, 26, 191–197. [Google Scholar] [CrossRef]
- Narahashi, T.; Moore, J.W.; Scott, W.R. Tetrodotoxin blockage of sodium conductance increase in lobster giant axons. J. Gen. Physiol. 1964, 47, 965–974. [Google Scholar] [CrossRef]
- Catterall, W.A.; Nirenberg, M. Sodium uptake associated with activation of action potential ionophores of cultured neuroblastoma and muscle cells. Proc. Natl. Acad. Sci. USA 1973, 70, 3759–3763. [Google Scholar] [CrossRef]
- Do, H.; Hamasaki, K.; Ohwada, K.; Simidu, U.; Noguchi, T.; Shida, Y.; Kogure, K. Presence of tetrodotoxin and tetrodotoxin-producing bacteria in freshwater sediments. Appl. Environ. Microbiol. 1993, 59, 3934–3937. [Google Scholar]
- Wu, Z.; Xie, L.; Xia, G.; Zhang, J.; Nie, Y.; Hu, J.; Wang, S.; Zhang, R. A new tetrodotoxin-producing actinomycete, Nocardiopsis dassonvillei, isolated from the ovaries of puffer fish Fugu rubripes. Toxicon 2005, 45, 851–859. [Google Scholar] [CrossRef]
- Gallacher, S.; Birkbeck, T. A tissue culture assay for direct detection of sodium channel blocking toxins in bacterial culture supernates. FEMS Microbiol. Lett. 1992, 92, 101–107. [Google Scholar] [CrossRef]
- Hamasaki, K.; Kogure, K.; Ohwada, K. A biological method for the quantitative measurement of tetrodotoxin (TTX): Tissue culture bioassay in combination with a water-soluble tetrazolium salt. Toxicon 1996, 34, 490–495. [Google Scholar] [CrossRef]
- Hamasaki, K.; Kogure, K.; Ohwada, K. An improved method of tissue culture bioassay for tetrodotoxin. Fish. Sci. 1996b, 62, 825–829. [Google Scholar]
- Kodama, M.; Ogata, T.; Noguchi, T.; Maruyama, J.; Hashimoto, K. Occurrence of saxitoxin and other toxins in the liver of the pufferfish Takifugu pardalis. Toxicon 1983, 21, 897–900. [Google Scholar] [CrossRef]
- Jang, J.; Yotsu-Yamashita, M. Distribution of tetrodotoxin, saxitoxin, and their analogs among tissues of the puffer fish Fugu pardalis. Toxicon 2006, 48, 980–987. [Google Scholar] [CrossRef]
- Jen, H.-C.; Lin, S.-J.; Lin, S.-Y.; Huang, Y.-W.; Liao, I.; Arakawa, O.; Hwang, D.-F. Occurrence of tetrodotoxin and paralytic shellfish poisons in a gastropod implicated in food poisoning in southern Taiwan. Food Add. Contam. 2007, 24, 902–909. [Google Scholar] [CrossRef]
- Yotsu-Yamashita, M.; Mebs, D. Occurrence of 11-oxotetrodotoxin in the red-spotted newt, Notophthalmus viridescens, and further studies on the levels of tetrodotoxin and its analogues in the newt’s efts. Toxicon 2003, 41, 893–897. [Google Scholar] [CrossRef]
- Cheun, B.; Endo, H.; Hayashi, T.; Nagashima, Y.; Watanabe, E. Development of an ultra high sensitive tissue biosensor for determination of swellfish poisoning, tetrodotoxin. Biosens. Bioelectron. 1996, 11, 1185–1191. [Google Scholar] [CrossRef]
- Pancrazio, J.J.; Gray, S.A.; Shubin, Y.S.; Kulagina, N.; Cuttino, D.S.; Shaffer, K.M.; Eisemann, K.; Curran, A.; Zim, B.; Gross, G.W. A portable microelectrode array recording system incorporating cultured neuronal networks for neurotoxin detection. Biosens. Bioelectron. 2003, 18, 1339–1347. [Google Scholar] [CrossRef]
- Charkhkar, H.; Knaack, G.L.; Gnade, B.E.; Keefer, E.W.; Pancrazio, J.J. Development and demonstration of a disposable low-cost microelectrode array for cultured neuronal network recording. Sens. Actuators B 2012, 161, 655–660. [Google Scholar] [CrossRef]
- Mohan, D.K.; Molnar, P.; Hickman, J.J. Toxin detection based on action potential shape analysis using a realistic mathematical model of differentiated NG108-15 cells. Biosens. Bioelectron. 2006, 21, 1804–1811. [Google Scholar] [CrossRef]
- Weiser, T. A novel toxicity-based assay for the identification of modulators of voltage-gated Na+ channels. J. Neurosci. Methods 2004, 137, 79–85. [Google Scholar] [CrossRef]
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