Krempfielins J–M, New Eunicellin-Based Diterpenoids from the Soft Coral Cladiella krempfi

New four eunicellin-based diterpenoids, krempfielins J–M (1–4) were isolated from the organic extract of a Taiwanese soft coral Cladiella krempfi. The structures of the new metabolites were elucidated on the basis of extensive spectroscopic analysis. The structure of compound 2 is rare due to the presence of the highly oxygenated pattern. Anti-inflammatory activity of 1–6 to inhibit the superoxide anion generation and elastase release in FMLP/CB-induced human neutrophils was also evaluated, and 2 and 4 were shown to possess the ability to inhibit the elastase release.


Results and Discussion
Krempfielin J (1) showed the pseudomolecular ion peak [M + Na] + at m/z 417.2616 in the HRESIMS and the molecular formula was determined as C 23 H 38 O 5 . NMR spectroscopic data of 1 (Table 1) showed the presence of one acetoxy group (δ C 169.6 and 22.4; δ H 2.12, s, 3H) and one methoxyl group (δ C 57.0 and δ H 3.34, 3H, s). The NMR data of 1 was found to be similar to the known compound, 7 [22,25] (Chart 1). Comparison of the NMR data of them revealed that the only difference between both compounds arises from the replacement of the n-butyryloxy group at C-3 in 7 by one acetoxy group in 1. The stereochemistry of 1 was confirmed by comparison of the NMR data and NOE correlations of both 7 and 1. The new metabolite krempfielin K (2) was found to have the molecular formula C 26 H 42 O 8 and six degrees of unsaturation, as indicated from the HRESIMS. The IR absorptions at ν max 3444 and 1732 cm −1 revealed the presence of hydroxy and ester carbonyl functionalities. The 13 C carbons spectral data (Table 1) were assigned by the assistance of DEPT spectrum to six methyls (including one acetate methyl δ C 21.2), five sp 3 methylenes, one sp 2 methylene, nine sp 3 methines (including five oxymethines), two sp 3 and three sp 2 quaternary carbons (including two ester carbonyls). The NMR spectroscopic data of 2 (Table 1) showed the presence of one 1,1-disubstituted double bond (δ C 105.0, CH 2 and 145.3, C; δ H 5.23, s and 5.04, s, each 1H). Two ester carbonyls (δ C 172.6 and 170.0) were assigned from the 13 C NMR spectrum and were HMBC correlated with the methylene (δ H 2.30 m and 2.22 m, each 1H) of an n-butyrate and those of an acetate methyl (δ H 2.14, s, 3H), respectively. Therefore, the remaining three degrees of unsaturation identified compound 2 as a tricyclic molecule. 1 H-1 H COSY and HMBC correlations ( Figure 1) were further used to establish the molecular skeleton of 2. The COSY experiment assigned five isolated consecutive proton spin systems. Above evidences and HMBC correlations from H-2 and H-6 (δ H 3.58 and 3.68) to C-6 and C-2 (δ C 70.1 and 80.9), respectively, implied the presence of an ether linkage between C-2 and C-6, and suggested that 2 is an 2,6-ether linked eunicellin-based diterpenoid [1,26]. Furthermore, the acetoxy group attaching at C-12 was confirmed from the HMBC correlations from H-12 (δ H 5.29) and acetate methyl protons (δ H 2.14) to the carbonyl carbon appearing at 170.0 (C). Thus, the remaining one n-butyryloxy group had to be positioned at C-3, an oxygen-bearing quaternary carbon resonating at δ 83.8 ppm. On the basis of above analysis, the planar structure of 2 was established. The relative structure of 2 was elucidated by the analysis of NOESY correlations, as shown in Figure 2 (Table 1), which were characterized by the DEPT spectrum as five methyls (including one acetate methyl), five methylenes (including one sp 2 methylene), eight methines (including four oxygenated carbons), and four quaternary carbons (including one ester carbonyl and one sp 2 quaternary carbon of an olefin). The 1 H and 13 C NMR spectral data of 3 (Table 1) also showed the presence of one acetoxy group (δ H 2.07, s, 3H; δ C 22.4, CH 3 and 169.5, C). The remaining three degrees of unsaturation again identified 3 as a tricyclic diterpenoid. The molecular framework was established by 1 H-1 H COSY and HMBC correlations (Figure 1). Comparison of the NMR data of 3 with those of the known compound sclerophytin E [27] revealed that 3 is the C-12 hydroxylated derivative of sclerophytin E. The stereochemistry of compound 3 was determined by the NOESY spectrum as shown in Figure 2.  (Table 1) revealed that the structure of metabolite 4 should be similar to that of 3, as the 1 H NMR spectral data of 4 are almost identical with those of 3 except for the presence of one additional acetyl group (δ H 2.06, s, 3H) in 4. Furthermore, the placement of an acetoxy group at C-12 was established by the HMBC experiment which showed correlations from an oxymethine (δ H 5.44, H-12) and acetate methyl (δ H 2.06) to the ester carbonyl carbon appearing at δ C 170.3 (C) (Figure 1). The NOE correlations of 4 also showed that the stereochemistry of this metabolite is identical with that of 3. Thus the structure of metabolite 4 was determined.
The in vitro anti-inflammatory effects of the diterpenoids 16 were tested by examining the inhibitory activity of these compounds toward the release of elastase in N-formyl-methionyl-leucyl-pheny lalanine/cytochalasin B (FMLP/CB)-induced human neutrophils cells (Table 2). At a concentration of 10 μM, all of these compounds could not significantly reduce the expression of superoxide anion, relative to the control cells stimulated with FMLP/CB only. At the same concentration, compounds 2 and 4 were found to effectively inhibit the elastase release (45.51 ± 2.69% and 27.30 ± 5.42% inhibition, respectively) in the same FMLP/CB-stimulated cells. Percentage of inhibition (Inh%) was measured at 10 μM. Results are presented as mean ± S.E.M. (n = 3 or 4); * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with the control value.

General Experimental Procedures
Melting point was determined using a Fisher-Johns melting point apparatus. Optical rotations were measured on a JASCO P-1020 polarimeter. IR spectra were recorded on a JASCO FT/IR-4100 infrared spectrophotometer. ESIMS were obtained with a Bruker APEX II mass spectrometer. The NMR spectra were recorded either on a Varian UNITY INOVA-500 FT-NMR and a Varian 400MR FT-NMR. Silica gel (Merck, 230-400 mesh) was used for column chromatography. Precoated silica gel plates (Merck, Kieselgel 60 F-254, 0.2 mm) were used for analytical thin layer chromatography (TLC). High performance liquid chromatography was performed on a Hitachi L-7100 HPLC apparatus with an octadecylsilane (ODS) column (250 × 21.2 mm, 5 μm).

Animal Material
C. krempfi was collected by hand using scuba off the coast of Penghu islands of Taiwan in June 2008, at a depth of 5-10 m, and stored in a freezer until extraction. A voucher sample (specimen No. 200806CK) was deposited at the Department of Marine Biotechnology and Resources, National Sun Yat-sen University.

Extraction and Separation
The octocoral (1.1 kg fresh wt) was collected and freeze-dried. The freeze-dried material was minced and extracted exhaustively with EtOH (3 × 10 L). The EtOH extract of the frozen organism was partitioned between CH 2 Cl 2 and H 2 O. The CH 2 Cl 2 -soluble portion (14.4 g) was subjected to column chromatography on silica gel and eluted with EtOAc in n-hexane (0%-100% of EtOAc, stepwise) and then further with MeOH in EtOAc with increasing polarity to yield 41 fractions. Fraction 28, eluted with n-hexane-EtOAc (1:2), was rechromatoraphed over a reversed-phase column (RP-18) using acetone-H 2 O (10:1) as the mobile phase to afford six subfractions (A1-A6). Subfraction A3 was repeatedly separated by reverse phase HPLC (CH 3

In Vitro Anti-Inflammatory Assay-Superoxide Anion Generation and Elastase Release by Human Neutrophils
Human neutrophils were obtained by means of dextran sedimentation and Ficoll centrifugation. Measurements of superoxide anion generation and elastase release were carried out according to previously described procedures [28,29]. LY294002, a phosphatidylinositol-3-kinase inhibitor, was used as a positive control for inhibition of superoxide anion generation and elastase release with IC 50 values of 1.88 ± 0.45 and 4.12 ± 0.92 μM, respectively. Briefly, superoxide anion production was assayed by monitoring the superoxide dismutase-inhibitable reduction of ferricytochrome c. Elastase release experiments were performed using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as the elastase substrate [30].

Conclusions
New eunicellin-based diterpenoids were isolated together with known ones from the soft coral Cladiella krempfi. Compounds 2 and 4 could significantly inhibit the release of elastase in FMLP/CB-induced human neutrophils. Thus, compounds 2 and 4, in particular 2, could be promising anti-inflammatory agents and may warrant further biomedical investigation.