Pullularins E and F, Two New Peptides from the Endophytic Fungus Bionectria ochroleuca Isolated from the Mangrove Plant Sonneratia caseolaris

Chemical investigation of the EtOAc extract of the endophytic fungus Bionectria ochroleuca, isolated from the inner leaf tissues of the plant Sonneratia caseolaris (Sonneratiaceae) from Hainan island (China), yielded two new peptides, pullularins E and F (1 and 2) together with three known compounds (3–5). The structures of the new compounds were unambiguously determined on the basis of one- and two-dimensional NMR spectroscopy as well as by high-resolution mass spectrometry. The absolute configurations of amino acids were determined by HPLC analysis of acid hydrolysates using Marfey’s method. The isolated compounds exhibited pronounced to moderate cytotoxic activity against the mouse lymphoma cells (L5178Y) with EC50 values ranging between 0.1 and 6.7 µg/mL.

, containing one chlorine atom. Therefore, compound 1 was totally transformed to a chloro-derivative (1a). Compound 1a was re-measured in DMSO-d 6 to get better resolution of the NMR spectra. Extensive analysis of the NMR data of 1a and comparison with those reported for pullularins A (3) and C (4) [17] indicated a close structural relationship of 1a with the latter depsipeptides. The number of hydrogen and carbon atoms observed in the 1 H and 13 C NMR spectra of 1a was in agreement with the molecular formula, indicating that 1a is a hexadepsipeptide composed of one 2-hydroxycarboxylic acid moiety and five amino acid residues. Correspondingly, one ester carbonyl carbon (δ C 169.5) and five amide carbonyl carbons (δ C 168. 1, 165.4, 171.8, 173.8 and 168.0) were discernible. Since only two -NH proton signals (δ H 9.53 and 8.89) and two N-methyl groups (δ H 2.93 and 2.40) were observed in the 1 H NMR spectrum of 1a, the fifth amino acid was assumed to represent proline. This assumption was corroborated by analysis of the TOCSY spectrum, which in addition allowed for assigning the spin systems and furthermore hinted at the presence of an O-isoprenyl residue. The positions of the N-methyl groups, the prenyl residue, the sequence of the amino acid residues and the 2-hydroxycarboxylic acid were established by extensive analysis of the HMBC and ROESY data (Table 1).  methylene protons were referred as "a" in upper row, and "b" in the next row; d Assignments within a column maybe interchanged.
The 2-hydroxycarboxylic acid was assigned to be 3-phenyllactic acid (3-Ph-Lac), on the basis of an oxymethine (δ C 71.5) attached to a methylene at δ C 35.8, which in turn was adjacent to a monosubstituted phenyl group. Extensive analysis of the NMR data showed that the serine residue in the known compound 3 was replaced by an alanine residue in 1a, as evidenced by the upfield shifted signals at δ H 4.65 of the α-proton and at δ H 1.21 of the aliphatic methyl group in the alanine residue. Its -NH group at δ H 8.89 showed correlations to the methyl group and the carbonyl groups of proline, indicating that both amino acids were adjacent.
The NMR spectra of 1a differed from those of 3 and 4 furthermore by the nature of the isoprene unit. Instead of two olefinic methyl groups as present in compounds 3 and 4 only one signal was found for 1a at δ H 1.80, sharing a COSY cross peak with the methylene function CH 2 -4′ at (δ H 5.20 and 5.04). The olefinic methyl proton signal also correlated with a quaternary carbon C-3′ at δ C 141.3 and a methine carbon C-2′ at δ C 63.0 in the HMBC spectrum. Moreover, a chlorine was assumed to be attached at C-2′, based on the chemical shifts of CH-2′ (δ C 63.0, δ H 4.86). Thus, 1a featured an O-isopentenyl moiety as isoprene substituent instead of an O-dimethylallyl residue as present in 3 and 4.
For the determination of the stereochemistry of the amino acid residues, the so-called advanced Marfey's method was employed [19]. After hydrolysis of 1a in 6 M HCl for 24 h, the hydrolyzate was transformed to diastereomeric reaction products by adding the reagent FDAA. The reaction products thus obtained were submitted to analytical HPLC and LCMS. Moreover, the commercially available amino acids tyrosine, N-methyl-isoleucine, alanine, proline and N-methyl-alanine were used as authentic standards, both as the respective L-enantiomers and as racemates, and subjected to similar conditions for the derivatization and analysis. By comparison of the retention times of the reaction products of the hydrolyzate and the amino acid standards, the configuration of the respective amino acid was determined. As tyrosine lost its isoprenyl residue during hydrolysis, it was not necessary to provide O-isoprenyl tyrosine derivatives. On this basis, the configurations of the amino acids in 1a were determined as L-Tyr, N-Me-L-Ile, L-Ala, L-Pro and N-Me-L-Ala.
1a is obviously an artifact. Unfortunately, it was not possible to collect the NMR data of 1 in other deuterated solvents, since it was totally transformed to 1a. It was interesting that the transformation to a chloro-derivative was only observed in 1 despite that compounds 3 and 4 also have double bond in the isoprenyl residue and were also measured in CDCl 3 . The structure of the new compound 1 was tentatively deduced as shown in Figure 1. The compound could not be dissolved in CDCl 3 as for the other pullularins, but only in DMSO. Signals of the amino acid residues for proline, serine, N-methylated isoleucine and O-prenyl-tyrosine were readily observed, in the NMR spectra of 2 as well as signals for 3-phenyllactic acid, proving the absence of N-methylated alanine in 2. A conspicuous feature of the NMR spectra was the upfield shift of H-2 of 3-Ph-Lac to δ H 4.10 as compared to other pullularins, indicating the presence of an alcohol function instead of an ester moiety. This finding and the lack of correlations between the α-hydroxycarboxylic acid and the O-prenyl-tyrosine indicated that pullularin F (2) was a linear and not a cyclic peptide. This finding was also consistent with the molecular formula obtained from HRESIMS. Moreover, it also explained the increase in polarity of 2 in comparison to pullularins A (3), C (4) and chloro-derivative of pullularin E (1a).
The peptide was hydrolyzed and the stereochemistry of the amino acids analyzed also via Marfey's method [19]. On this basis, the configurations of the amino acids in 2 were determined as L-Tyr, N-Me-L-Ile, L-Ser and L-Pro.
All isolated compounds were subjected to a cytotoxicity assay employing the murine lymphoma L5178Y cell line, which is summarized in Table 2. Verticillin D (5) showed pronounced cytotoxic activities against the tested cell line. Antiproliferative properties were also prevalent among the cyclic depsipeptides pullularin A (3), C (4) and chloro-derivative of pullularin E (1a) with EC 50 values ranging between 0.1 and 6.7 µg/mL, whereas the linear pullularin F (2) did not exhibit any cytotoxic activity at the tested dose.

General Experimental Procedures
Optical rotations were measured on a Perkin-Elmer-241 MC polarimeter. 1D and 2D NMR spectra were recorded on Bruker ARX 500, ARX 400 or AVANCE DMX 600 NMR spectrometers. ESIMS and HRESIMS were obtained on Finnigan LCQ Deca and Micromass Qtof 2 mass spectrometers, respectively. Solvents were distilled prior to use, and spectral grade solvents were used for spectroscopic measurements.

Fungal Material
Fresh, healthy leaves of Sonneratia caseolaris (Sonneratiaceae) were collected in September 2009 from Hainan Island of the Dongzhai Mangrove Forest. Leaves were rinsed twice with sterilized distilled water. Surface sterilization was achieved by immersing the leaves in 70% ethanol for 2 min (twice) followed by rinsing twice in sterilized distilled water. Then, the leaves were cleaved aseptically into small segments (approx. 1 cm in length). The material was placed on a Petri dish (malt agar medium) containing an antibiotic to suppress bacterial growth (medium composition: 15 g/L malt extract, 15 g/L agar, 24.4 g/L sea salt, and 0.2 g/L chloramphenicol in distilled water, pH 7.4-7.8) and incubated at room temperature (25 °C). After several days, hyphae growing from the plant material were transferred to fresh plates with the same medium, incubated again for 10 days, and periodically checked for culture purity.

Identification of Fungal Cultures
Fungal cultures were identified according to a molecular biological protocol by DNA amplification and sequencing of the ITS region as described previously [20]. The sequence data have been submitted to GenBank, accession number JQ407533. The fungal strain was identified as Bionectria ochroleuca. A voucher strain (strain designation JCM 10.3) is kept in the Institute of Pharmaceutical Biology and Biotechnology, Duesseldorf, Germany.

Cultivation
Twenty Erlenmeyer flasks (1 L each) containing 100 g of rice and 110 mL of distilled water were autoclaved. A small part of the medium from a Petri dish containing the purified fungus was transferred under sterile conditions to the rice medium. The fungal strain was grown on solid rice medium at room temperature (22 °C) for 40 days.

Preparation and HPLC Analysis of Marfey Derivatives
The isolated peptide was hydrolyzed (0.5-1 mg) with 1-2 mL 6N HCl at 110 °C for 24 h under N 2 atmosphere. The hydrolysate containing a mixture of free amino acids was cooled, dried and redissolved in water. Derivatization was achieved in the same manner as applied to standard amino acids. The retention times of the derivatized standard amino acids and of the derivatized amino acids obtained following hydrolysis of the peptide were compared to distinguish D-and L-amino acids.
Pullularin   128.0 CH 7.26 m * a Measured at 600 ( 1 H) and 150 ( 13 C) MHz; b The assignments for methylene protons were referred as "a" in upper row, and "b" in the next row; * Overlapped signals.

Cell Proliferation Assay
Cytotoxicity was tested against the L5178Y mouse lymphoma cell line using the microculture tetrazolium (MTT) assay [22,23]. Experiments were repeated three times and carried out in triplicate. As negative controls, media with 0.1% (v/v) EtOH were included in all experiments.