Evaluating the Impact of Oleocanthal and Oleacein on Skin Aging: Results of a Randomized Clinical Study

The prevalence of skin aging and the request for effective treatments have driven dermatological research towards natural solutions. This study investigates the anti-aging efficacy of two bioactive natural polyphenols, Oleocanthal and Oleacein, in a skincare formulation. A single-blind, randomized clinical trial involved 70 participants, using a comprehensive exclusion criterion to ensure participant safety and study integrity. Participants applied the Oleocanthal and Oleacein 1% serum formulation twice daily for 30 days. The efficacy was objectively assessed using the VISIA® Skin Analysis System at baseline, after 15 days, and after 30 days. Results indicated significant wrinkle reduction in most groups. For women aged 45–79 years, the mean change was −33.91% (95% CI: −46.75% to −21.07%). For men aged 20–44 years, it was −51.93% (95% CI: −76.54% to −27.33%), and for men aged 45–79 years, it was −46.56% (95% CI: −58.32% to −34.81%). For women aged 20–44 years, the change was −25.68% (95% CI: −63.91% to 12.54%), not statistically significant. These findings highlight the potential of EVOO-derived polyphenols in anti-aging skincare, particularly for older adults. This research paves the way for further exploration into natural compounds in dermatology, particularly for aging skin management.


Introduction
The pursuit of natural, effective anti-aging treatments is a growing area of interest, particularly in the field of dermatology.
Wrinkles, a key indicator of skin aging, arise from both intrinsic factors, such as changes in dermal and epidermal structures, and extrinsic factors like UV exposure and lifestyle choices.These factors contribute to decreased fibroblast activity and reduced collagen production, accelerating wrinkle formation.
Extra Virgin Olive Oil (EVOO) and its extracts play a central role in counteracting aging sign-It is recognized not only for its role in the Mediterranean diet but also for its broader health benefits.These benefits have been shown in many studies and include cardiovascular protection, anti-inflammatory effects, and metabolic health benefits [1][2][3][4][5].Actuality, olive oil consumption can lessen inflammation, endothelial dysfunction, lipid and DNA oxidation, lipid profile, insulin resistance, and blood pressure in hypertensive people.These benefits guard against metabolic abnormalities as well as cardiovascular disease [6].
Additionally, it has been discovered that fatty acids and monoglycerides exhibit a wide range of microbicidal activity against yeasts and bacteria [10].Additionally, it has been shown that the α,β-unsaturated aldehydes found in olives and olive oil flavor have a discernible effect on pathogens that affect the human respiratory and intestinal tracts [11].
Concerns regarding phenolic compounds from plants and foods that may alter gut microbiota by favoring the growth of lactobacilli and bifidobacteria while suppressing the growth of pathogenic bacteria like clostridia have been raised in relation to human health [12,13].
These compounds have also showed potential in improving dermatological signs related to aging and in particular [14] diminishing wrinkle appearance, positioning EVOO as a key focus in anti-aging research.
Oleocanthal and oleacein, compounds derived from EVOO, have shown promise in skin health enhancement, particularly in treating non-melanoma skin cancers and facilitating wound healing [15,16].Their effectiveness aligns with broader research on the skin health benefits of polyphenols, including their ability to penetrate epidermal barriers and interact with cellular receptors.
This study focuses for the first time on a detailed evaluation of a dermatological serum containing Oleocanthal and Oleacein, EVOO-derived polyphenols, focusing on its anti-wrinkle efficacy through multispectral analysis.Our aim is to provide a comprehensive, scientifically robust analysis of wrinkle reduction across various demographics, contributing to the field of natural anti-aging solutions.
EVOO, with its rich composition of beneficial compounds, is proving to be a versatile agent in skin health, extending beyond dietary benefits to topical applications.The polyphenols Oleocanthal and Oleacein, two polyphenols derived from EVOO have been incorporated into skincare products to gain more natural, sustainable approaches in antiaging treatments.Moreover, traditional antiaging compounds are being enhanced by natural ingredients and innovative technologies, paving the way for more effective, holistic treatment strategies.This convergence of natural ingredients and advanced technology is not only redefining treatment protocols but also aligning with a growing consumer preference for sustainable, health-conscious solutions in skincare and medical treatments.
In this open randomized clinical study, we investigate the antiaging efficacy of Oleocanthal and Oleacein in an innovative skincare formulation.
While this study provides valuable insights into the efficacy of Oleocanthal and Oleacein, it is important to note the limitations associated with the functional status of patients, including issues related to sensitization.Participants' skin sensitivity and reactions to the serum formulation can vary widely, potentially affecting the outcomes.Sensitization to active ingredients is a critical factor that can influence the effectiveness and safety of dermatological treatments.Therefore, careful monitoring and consideration of individual skin reactions are essential to ensure accurate and reliable results.

Study Design and Setting
This study was a prospective, controlled, randomized, single-blind clinical trial conducted at the Dermatology Units of Magna Graecia University in Catanzaro and Sapienza University in Rome, Italy.The study was a prospective, controlled, randomized single-blind clinical trial conducted at the Dermatology Units of Magna Graecia University in Catanzaro and Sapienza University in Rome, Italy.The study was carried out from May to June 2023 targeting individuals with mild to moderate signs of aging.The participants were divided into subgroups based on age and skin morphotype: women (31 aged 20-44 years, 24 aged 45-79 years) and men (8 aged 20-44 years, 7 aged 45-79 years).Each subgroup was evenly distributed between 'thin skin' and 'thick skin' morphotypes.

Participants
Seventy healthy immunocompetent males and females aged between 18 and 75 years were recruited.The sample is divided into women, with 31 participants aged 20-44 years and 24 participants aged 45-79 years, evenly distributed across two morphotype groups: 'thin skin' and 'thick skin'.The sample also includes men, with 8 participants aged 20-44 years and 7 participants aged 45-79 years, similarly distributed across the two morphotype groups.The classification of skin morphotype was determined using highfrequency ultrasound, which measured the epidermal and dermal thickness of the facial skin.Mean values of epidermal thickness were approximately 0.18 mm, while mean values of dermal thickness were around 0.98 mm.Participants with dermal thickness values significantly above or below these means were classified as having 'thick skin' or 'thin skin,' respectively.The study enforced strict exclusion criteria to ensure the reliability and safety of the results.Excluded were individuals with extremely mild or severe skin aging, a history of skin cancer on the face or scalp, systemic tumors, dermatological conditions.

Informed Consent and Data Collection
All participants provided informed consent at their baseline visit.Detailed data, including age, Fitzpatrick skin phototype, medical history, chronic medication use, smoking and alcohol habits, weight, and height, were meticulously recorded.The study adhered to current legislation for observational studies, Good Clinical Practice (GCP) guidelines, and the ethical principles of the Declaration of Helsinki.

Evaluation Schedule
Patient evaluations occurred at three key intervals: baseline (t0), after 15 days (t1), and after 30 days (t2) of product usage.The first follow-up visit was conducted after 15 days, with 50 participants completing the third visit.

Objective Assessment
Skin aging improvement was objectively evaluated using the VISIA ® Skin Analysis System (Canfield Scientific, Parsippany, NJ, USA).This system provided a comprehensive and objective measurement of the treatment's impact on skin, assessing both the total area of wrinkles and their intensity.The VISIA ® system is validated through multiple studies and offers a detailed visualization of skin roughness and treatment progress.

Statistical Analysis
For this study, we used specialized statistical software SPSS (Statistical Package for the Social Sciences) that was fully compatible with the VISIA® Skin Analysis System to analyze the data.This software enabled the precise quantification of various skin parameters with a particular focus on wrinkle metrics.To determine statistical significance, 95% Confidence Intervals (CIs) were calculated for key metrics, including mean wrinkle count and percentage change from baseline.A 95% CI that did not include zero was considered indicative of statistically significant changes.
Data were presented in two formats: absolute scores and percentage changes from the baseline, allowing for a nuanced comparison of wrinkle progression throughout the treatment period.We conducted an inclusive analysis of both superficial and deep wrinkles to provide a comprehensive assessment of Oleocanthal and Oleacein serum efficacy.Recognizing the importance of gender-specific responses, separate analyses for male and female participants were also carried out.
To facilitate the interpretation of our findings, graphical data such as forest plots were used.This visual representation helped in highlighting key trends and patterns.The study collected longitudinal data at three time points-baseline, 15 days, and 30 days post-treatment initiation-enabling an evaluation of the treatment's efficacy over time.

Results
The data from this study offer robust evidence supporting the efficacy of 1% Oleocanthal and Oleacein serum in reducing wrinkles.Employing the VISIA ® imaging system for precise measurements, we observed a significant decrease in wrinkle count across all participants (Figures 2-5).Notably, there was an average percentage reduction of 23.1% in wrinkle count at the end of the 30-day period compared to baseline measurements.
A consistent trend of decreasing wrinkle counts was evident at each of the three evaluation intervals: baseline, 15 days, and 30 days (Figure 1).Specifically, for men, the median wrinkle count at the start was 83,232, which reduced to 70,568 at day 15, and further to 56,735 by day 30.Similarly, for women, the median count decreased from 76,369 at baseline to 70,441 at day 15, and then to 61,491 at day 30.This trend demonstrates not only the effectiveness of the treatment in wrinkle reduction but also its sustained impact over the course of the study.
Data were presented in two formats: absolute scores and percentage changes from the baseline, allowing for a nuanced comparison of wrinkle progression throughout the treatment period.We conducted an inclusive analysis of both superficial and deep wrinkles to provide a comprehensive assessment of Oleocanthal and Oleacein serum efficacy.Recognizing the importance of gender-specific responses, separate analyses for male and female participants were also carried out.
To facilitate the interpretation of our findings, graphical data such as forest plots were used.This visual representation helped in highlighting key trends and patterns.The study collected longitudinal data at three time points-baseline, 15 days, and 30 days posttreatment initiation-enabling an evaluation of the treatment's efficacy over time.

Results
The data from this study offer robust evidence supporting the efficacy of 1% Oleocanthal and Oleacein serum in reducing wrinkles.Employing the VISIA ® imaging system for precise measurements, we observed a significant decrease in wrinkle count across all participants (Figures 2-5).Notably, there was an average percentage reduction of 23.1% in wrinkle count at the end of the 30-day period compared to baseline measurements.
A consistent trend of decreasing wrinkle counts was evident at each of the three evaluation intervals: baseline, 15 days, and 30 days (Figure 1).Specifically, for men, the median wrinkle count at the start was 83,232, which reduced to 70,568 at day 15, and further to 56,735 by day 30.Similarly, for women, the median count decreased from 76,369 at baseline to 70,441 at day 15, and then to 61,491 at day 30.This trend demonstrates not only the effectiveness of the treatment in wrinkle reduction but also its sustained impact over the course of the study.

Generalizability of Findings
The findings were further substantiated by the Interquartile Range (IQR) s which revealed that the positive outcomes of the treatment were consistent different demographic segments.This indicates that Oleocanthal and Olea effectiveness in wrinkle reduction is not confined to a specific subgroup but is b applicable, thereby enhancing the generalizability of the results.The significant red in wrinkle counts in both male and female participants underscore the serum cont Oleocanthal and Oleacein's potential as an effective anti-wrinkle treatment.

Number of Wrinkles: Absolute Scores
Detailed data on the number of wrinkles will be presented here, captured at di

Generalizability of Findings
The findings were further substantiated by the Interquartile Range (IQR) scaling, which revealed that the positive outcomes of the treatment were consistent across different demographic segments.This indicates that Oleocanthal and Oleacein's effectiveness in wrinkle reduction is not confined to a specific subgroup but is broadly applicable, thereby enhancing the generalizability of the results.The significant reduction in wrinkle counts in both male and female participants underscore the serum containing Oleocanthal and Oleacein's potential as an effective anti-wrinkle treatment.

Number of Wrinkles: Absolute Scores
Detailed data on the number of wrinkles will be presented here, captured at different time intervals: baseline, day 15, and day 30.The data will be recorded as "absolute scores", providing a precise quantitative snapshot of the participants' skin conditions.Both surface wrinkles and deep wrinkles will be included, as measured by the VISIA ® camera system.The following Table 1 presents the absolute scores for wrinkle count at baseline, day 15, and day 30.

Number of Wrinkles: Percent Change from Baseline
This section will delve into the percentage change in the number of wrinkles from the baseline.It will provide a clear picture of the treatment's efficacy over time.The data will be broken down by gender to highlight any differences in treatment response between men and women (Table 2).N: This represents the total number of subjects or samples included in the analysis.A larger sample size can provide more accurate estimates.Mean: This is the average value of the sample.In the context of this study, it represents the average percentage change in the number of wrinkles.CI (Confidence Interval): This is a range within which the true mean value (of the population) is expected to occur 95% of the time if the study were repeated many times.The lower and upper confidence interval provides a range of values that are statistically probable given the measures taken.If the confidence interval for the mean does not include zero, it suggests that there is a significant difference.

Gender-Specific Results
Below are the tables with the results, organized as follows: Women aged 20-44 years with thick skin (Table 3), Women aged 20-44 years with thin skin (Table 4), Women aged 45-79 years with thin skin (Table 5), Women aged 45-79 years with thick skin (Table 6), Men aged 20-44 years with thin skin (Table 7), Men aged 20-44 years with thick skin (Table 8), Men aged 45-79 years with thin skin (Table 9), and Men aged 45-79 years with thick skin (Table 10).Each table includes information on age, morphotype according to skin thickness, and wrinkle measurements at time points T0, T1, and T2.Additionally, the tables report the variations in values for the intervals: variation T0-T1, variation T1-T2, and variation T0-T2.Graphical representations have been included to enhance data visualization (Figures 6-13).These figures, presented as multiple bar charts, illustrate the changes in wrinkle measurements over time and the variations between these points, providing a clearer depiction of the results.4.This graph shows visually the results on the sample of women aged 20-44 years with thin skin at time 0, 1, and 2.   4.This graph shows visually the results on the sample of women aged 20-44 years with thin skin at time 0, 1, and 2.   4.This graph shows visually the results on the sample of women aged 20-44 years with thin skin at time 0, 1, and 2.  5.This graph shows visually the results on the sample of women aged 45-79 years with thin skin at time 0, 1, and 2.  6.This graph shows visually the results on the sample of women aged 45-79 years with thick skin at time 0, 1, and 2.  6.This graph shows visually the results on the sample of women aged 45-79 years with thick skin at time 0, 1, and 2.   6.This graph shows visually the results on the sample of women aged 45-79 years with thick skin at time 0, 1, and 2.  7.This graph shows visually the results on the sample of men aged 20-44 years with thin skin at time 0, 1, and 2.    8.This graph shows visually the results on the sample of men aged 20-44 years with thick skin at time 0, 1, and 2.  8.This graph shows visually the results on the sample of men aged 20-44 years with thick skin at time 0, 1, and 2.

Figure 12.
Graph related to the data exhibited in Table 9.This graph shows visually the results on the sample of men aged 45-79 years with thin skin at time 0, 1, and 2.
-5000 0 man man man man man man man man man man man man man man man man man man  9.This graph shows visually the results on the sample of men aged 45-79 years with thin skin at time 0, 1, and 2.   10.This graph shows visually the results on the sample of men aged 45-79 years with thick skin at time 0, 1, and 2.

Men's Data Analysis
The data shows a clear reduction in the number of wrinkles in male patients over 30 days of treatment.While there is considerable variability among individuals, the median and mean values demonstrate consistent decrease in wrinkle count from baseline through 15 days to 30 days across all measured facial areas.After 30 days, the average reduction in wrinkle count for men aged 20-44 years was −51.93% (95% CI: −76.54% to −27.33%), and for men aged 45-79 years, it was −46.56% (95% CI: −58.32% to −34.81%), indicating that the treatment is generally effective in reducing wrinkle count in male patients.

Women's Data Analysis
The data analysis for women reveals a reduction in average wrinkle counts after 15 and 30 days of treatment.Baseline median wrinkle count was 76,369, which decreased to 70,441 after 15 days, and further to 61,491 after 30 days.For women aged 45-79 years, the mean percentage change in wrinkle count was −33.91% (95% CI: −46.75% to −21.07%), indicating a statistically significant reduction in wrinkles.However, for women aged 20-44 years, the change was −25.68% (95% CI: −63.91% to 12.54%), not statistically significant, suggesting a need for further research to understand the variability in this subgroup.
Our study demonstrates that Oleocanthal and Oleacein significantly reduce wrinkle count in both men and women, particularly in those aged 45-79 years.The data collected at three distinct time intervals reveals a consistent trend of wrinkle reduction.However, the overall findings support the use of EVOO-derived polyphenols in anti-aging skincare formulations.
Initial Counts: At the onset of the treatment, men had a median wrinkle count of 83,232, and women had 76,369.Mid-Treatment: After 15 days, the median wrinkle count decreased to 70,568 for men and 70,441 for women, indicating a positive treatment effect.End of Treatment: This trend continued through day 30, with the median wrinkle count further reducing to 56,735 for men and 61,491 for women.The Interquartile Range (IQR)  10.This graph shows visually the results on the sample of men aged 45-79 years with thick skin at time 0, 1, and 2.

Men's Data Analysis
The data shows a clear reduction in the number of wrinkles in male patients over 30 days of treatment.While there is considerable variability among individuals, the median and mean values demonstrate consistent decrease in wrinkle count from baseline through 15 days to 30 days across all measured facial areas.After 30 days, the average reduction in wrinkle count for men aged 20-44 years was −51.93% (95% CI: −76.54% to −27.33%), and for men aged 45-79 years, it was −46.56% (95% CI: −58.32% to −34.81%), indicating that the treatment is generally effective in reducing wrinkle count in male patients.

Women's Data Analysis
The data analysis for women reveals a reduction in average wrinkle counts after 15 and 30 days of treatment.Baseline median wrinkle count was 76,369, which decreased to 70,441 after 15 days, and further to 61,491 after 30 days.For women aged 45-79 years, the mean percentage change in wrinkle count was −33.91% (95% CI: −46.75% to −21.07%), indicating a statistically significant reduction in wrinkles.However, for women aged 20-44 years, the change was −25.68% (95% CI: −63.91% to 12.54%), not statistically significant, suggesting a need for further research to understand the variability in this subgroup.
Our study demonstrates that Oleocanthal and Oleacein significantly reduce wrinkle count in both men and women, particularly in those aged 45-79 years.The data collected at three distinct time intervals reveals a consistent trend of wrinkle reduction.However, the overall findings support the use of EVOO-derived polyphenols in anti-aging skincare formulations.
Initial Counts: At the onset of the treatment, men had a median wrinkle count of 83,232, and women had 76,369.Mid-Treatment: After 15 days, the median wrinkle count decreased to 70,568 for men and 70,441 for women, indicating a positive treatment effect.End of Treatment: This trend continued through day 30, with the median wrinkle count further reducing to 56,735 for men and 61,491 for women.The Interquartile Range (IQR) scaling suggests greater uniformity in treatment outcomes over time.This indicates that the treatment's efficacy is not limited to a specific subgroup but is broadly applicable, enhancing its generalizability.

Discussion
Extra Virgin Olive Oil (EVOO) is rich in biologically active compounds such as oleocanthal and oleacein [17,18].These compounds are known for their anti-inflammatory properties and their ability to inhibit COX-1 and COX-2 enzymes, mirroring the action of synthetic drugs like ibuprofen but without the associated side effects [19].Our findings align with recent studies like those by Melguizo-Rodríguez et al. [20] and Donato-Trancoso et al. [21], which explore the biological effects of olive tree derivatives on the skin.
As we have already mentioned, olive oil contains triglyceride, monoglyceride, and diglyceride fatty acids make up the majority of its composition.More than 230 different compounds make up EVOO's minor compounds, which make up 2% of its total weight.These include hydrocarbons, sterols, aliphatic alcohols, tocopherols, pigments, volatile compounds, and phenolic compounds, which primarily consist of flavonoids, lignans, phenolic acids, phenolic alcohols, and secoiridoids and are related to the significant antioxidant potential of phenolic compounds [22,23].
Furthermore, the complex interplay of genetic and environmental factors-like UV radiation and pollution-in skin aging suggests that these compounds can positively impact skin aging, slowing down the process and promoting healthier, younger-looking skin [24].
Our results are also consistent with existing scientific literature on the benefits of polyphenols for skin health and overall health.These findings offer strong proof that olive oil and its phenolic component can be utilized as an alternative dietary therapy to prevent and treat oxidative and inflammatory immunological processes [25].
Polyphenols are known to enhance the skin's endogenous antioxidant system and can cross epidermal barriers to interact with cellular receptors, thereby preventing various skin diseases.They have been observed to improve the structural organization of the dermis, crucial for its barrier function, and to increase dermis thickness and hydration levels, essential for maintaining healthy, well-functioning skin [26].
The anti-inflammatory properties of EVOO's polyphenols are significant in preventing and treating immune-mediated inflammatory diseases [27], supporting our findings on the skin health benefits of EVOO.EVOO's derivatives have a profound impact on immuneinflammatory responses, highlighting its potential in clinical applications related to skin health [28].The protective effects of EVOO on immune-mediated inflammatory responses align with our observations on skin aging [21].
Moreover, recent studies have highlighted the role of EVOO in enhancing skin repair mechanisms [29][30][31].The topical application of EVOO has been shown to accelerate wound healing processes, attributing to the stimulation of collagen synthesis and angiogenesis.This finding is particularly relevant considering the increasing prevalence of skin conditions requiring regenerative treatments.
The application of EVOO in cosmetic formulations has gained attention due to its ability to improve skin elasticity and texture.Studies suggest that regular use of EVOO-based products can lead to a significant reduction in the appearance of fine lines and wrinkles, offering a natural alternative to synthetic anti-aging products.
This research, carried out at the University La Sapienza of Rome, importantly contributes to our understanding of anti-aging skincare.Our study focused on the effects of Oleocanthal and Oleacein, key ingredients in a polyphenol rich EVOO skincare formulation.In a carefully designed single-blind, randomized trial involving 70 participants, we noted a significant reduction in wrinkles.For women aged 45-79 years, the mean change was −33.91% (95% CI: −46.75% to −21.07%).For men aged 20-44 years, it was −51.93% (95% CI: −76.54% to −27.33%), and for men aged 45-79 years, it was −46.56% (95% CI: −58.32% to −34.81%).For women aged 20-44 years, the change was −25.68% (95% CI: −63.91% to 12.54%), not statistically significant.These significant findings not only affirm the objectives of our study but also emphasize the value of natural compounds in dermatological treatments, particularly for aging skin management.The uniformity of these results across various genders and facial regions highlights the broad applicability of EVOO-derived polyphenols in anti-aging skincare.This aligns with, and contributes to, the growing evidence supporting the effectiveness of nature-based solutions in enhancing skin health.

Conclusions
This research rigorously evaluated the efficacy of Oleocanthal and Oleacein, an Active-Italia skincare product, in reducing wrinkles.Utilizing the VISIA ® imaging system, we observed significant decreases in wrinkle counts across most demographic groups over a 30-day period.The results, coupled with the product's safety and tolerability profile, establish Oleocanthal and Oleacein as a viable option for wrinkle reduction and overall skin health enhancement.
Future research should aim to understand the variability in response among younger women and explore the long-term effects of Oleocanthal and Oleacein.Additionally, examining the specific mechanisms by which oleocanthal and oleacein contribute to wrinkle reduction could offer valuable insights into new skincare innovations.The results, coupled with the product's safety and tolerability profile, establish Oleocanthal and Oleacein as a viable option for wrinkle reduction and overall skin health enhancement.
The study adds to the evidence supporting natural compounds in skincare, particularly underscoring the benefits of polyphenols.These compounds effectively penetrate epidermal barriers and interact at the cellular level, enhancing skin health.Their presence in Oleocanthal and Oleacein is a crucial factor in its efficacy.
Considering the promising outcomes of this study, it is imperative to explore the long-term effects of Oleocanthal and Oleacein.Future research should aim to understand its continued impact on skin health over extended periods and examine its synergistic effects when used in combination with other skincare treatments.Additionally, delving into the specific mechanisms by which oleocanthal and oleacein contribute to wrinkle reduction could offer valuable insights into new skincare innovations.
Oleocanthal and Oleacein not only demonstrates efficacy in wrinkle reduction but also aligns with the broader scientific understanding of skin health.Its successful application in this study suggests its potential for both cosmetic and therapeutic use.The integration of natural, scientifically proven ingredients like oleocanthal and oleacein into skincare products represents a significant step forward in the quest for more effective, natural antiaging solutions.This approach not only meets the growing consumer demand for products with natural ingredients but also opens new avenues for future dermatological research and product development.In conclusion, Oleocanthal and Oleacein show how well traditional skincare practices and modern science can work together, offering a compelling example of the advancements achievable in the realm of natural skincare solutions.
In conclusion, while this study provides valuable insights into the efficacy of Oleocanthal and Oleacein serum, its limitations include a short 30-day duration, potential biases

Figure 1 .
Figure 1.Change from Baseline in Wrinkle Count by Region and Gender.Figure 1. Change from Baseline in Wrinkle Count by Region and Gender.

Figure 1 .Figure 2 .
Figure 1.Change from Baseline in Wrinkle Count by Region and Gender.Figure 1. Change from Baseline in Wrinkle Count by Region and Gender.

Figure 3 .
Figure 3. (a) VISIA Image Analysis of Male Patient: T1-Detailed Mapping of Facial Wrinkles.(b) VISIA Image Analysis of Male Patient: T3-Detailed Mapping of Facial Wrinkles.

Figure 4 .
Figure 4. (a) VISIA Image Analysis of Male Patient: T1-Detailed Mapping of Facial Wrink VISIA Image Analysis of Male Patient: T3-Detailed Mapping of Facial Wrinkles.

Figure 5 .
Figure 5. (a) VISIA Image Analysis of Male Patient: T1-Detailed Mapping of Facial Wrinkles.(b) VISIA Image Analysis of Male Patient: T3-Detailed Mapping of Facial Wrinkles.

Figure 7 .
Figure 7. Graph related to the data exhibited in Table4.This graph shows visually the results on the sample of women aged 20-44 years with thin skin at time 0, 1, and 2.

Figure 8 .
Figure 8. Graph related to the data exhibited in Table5.This graph shows visually the results on the sample of women aged 45-79 years with thin skin at time 0, 1, and 2.

Figure 7 .
Figure 7. Graph related to the data exhibited in Table4.This graph shows visually the results on the sample of women aged 20-44 years with thin skin at time 0, 1, and 2.

Figure 7 .
Figure 7. Graph related to the data exhibited in Table4.This graph shows visually the results on the sample of women aged 20-44 years with thin skin at time 0, 1, and 2.

Figure 8 .Figure 8 .
Figure 8. Graph related to the data exhibited in Table5.This graph shows visually the results on the sample of women aged 45-79 years with thin skin at time 0, 1, and 2.

Figure 9 .
Figure 9. Graph related to the data exhibited in Table6.This graph shows visually the results on the sample of women aged 45-79 years with thick skin at time 0, 1, and 2.

Figure 10 .Figure 9 .
Figure 10.Graph related to the data exhibited in Table7.This graph shows visually the results on the sample of men aged 20-44 years with thin skin at time 0, 1, and 2.

Figure 9 .
Figure 9. Graph related to the data exhibited in Table6.This graph shows visually the results on the sample of women aged 45-79 years with thick skin at time 0, 1, and 2.

Figure 10 .Figure 10 .
Figure 10.Graph related to the data exhibited in Table7.This graph shows visually the results on the sample of men aged 20-44 years with thin skin at time 0, 1, and 2.

Figure 11 .
Figure 11.Graph related to the data exhibited in Table8.This graph shows visually the results on the sample of men aged 20-44 years with thick skin at time 0, 1, and 2.

Figure 11 .
Figure 11.Graph related to the data exhibited in Table8.This graph shows visually the results on the sample of men aged 20-44 years with thick skin at time 0, 1, and 2.

Figure 11 .
Graph related to the data exhibited in Table

Figure 12 .
Figure 12.Graph related to the data exhibited in Table9.This graph shows visually the results on the sample of men aged 45-79 years with thin skin at time 0, 1, and 2.

Figure 13 .
Figure 13.Graph related to the data exhibited in Table10.This graph shows visually the results on the sample of men aged 45-79 years with thick skin at time 0, 1, and 2.

Figure 13 .
Figure 13.Graph related to the data exhibited in Table10.This graph shows visually the results on the sample of men aged 45-79 years with thick skin at time 0, 1, and 2.

Table 1 .
Absolute scores for wrinkle count at baseline, day 15, and day 30.

Table 2 .
Number of Wrinkles-Percent Change from Baseline.

Table 10 .
Men aged 45-79 years with thick skin.Graph related to the data exhibited in Table3.This graph shows visually the results on the sample of women aged 20-44 years with thick skin at time 0, 1, and 2. Graph related to the data exhibited in Table3.This graph shows visually the results on the sample of women aged 20-44 years with thick skin at time 0, 1, and 2.