Use of Infrared Thermography in Medical Diagnosis, Screening, and Disease Monitoring: A Scoping Review

Thermography provides non-invasive, radiation-free diagnostic imaging. Despite the extensive literature on medical thermography, a comprehensive overview of current applications is lacking. Hence, the aim of this scoping review is to identify the medical applications of passive infrared thermography and to catalogue the technical and environmental modalities. The diagnostic performance of thermography and the existence of specific reference data are evaluated, and research gaps and future tasks identified. The entire review process followed the Joanna Briggs Institute (JBI) approach and the results are reported according to PRISMA-ScR guidelines. The scoping review protocol is registered at the Open Science Framework (OSF). PubMed, CENTRAL, Embase, Web of Science, OpenGrey, OSF, and PROSPERO were searched using pretested search strategies based on the Population, Concept, Context (PCC) approach. According to the eligibility criteria, references were screened by two researchers independently. Seventy-two research articles were identified describing screening, diagnostic, or monitoring studies investigating the potential of thermography in a total of 17,314 participants within 38 different health conditions across 13 therapeutic areas. The use of several camera models from various manufacturers is described. These and other facts and figures are compiled and presented in a detailed, descriptive tabular and visual format. Thermography offers promising diagnostic capabilities, alone or in addition to conventional methods.


Introduction
The control of body temperature has been an important health indicator for clinical diagnosis and treatment since the time of Hippocrates, and it is said that Hippocrates applied wet mud to the skin to observe the faster drying of a tumorous swelling [1].The skin temperature in a healthy person under standardized environmental conditions stays within a narrowly defined range and depends on the blood circulation in the lower layers of the skin.Therefore, deviations in surface temperature can have many causes, such as inflammation, malignancy, and infection [2].Medical infrared thermography (IRT) offers a fast, painless, non-contact, non-invasive, and radiation-free method of photographically imaging temperature differences of the skin surface, allowing arbitrary repetitions of recording [3].Thermal imaging of the body surface can detect minute changes in the underlying tissues, which indicates neurological and vascular, as well as metabolic pathologies.For this purpose, modern infrared thermal imaging cameras are used, which have a high temperature sensitivity and produce high-resolution thermal images.Thermographic devices detect the infrared radiation emitted by the body surface, which is invisible to humans, convert it into electrical signals, and a thermogram can be created, which shows temperatures pictorially in different colours or gray tones [4].Infrared thermography is All references obtained were transferred and stored in the RefWorks web-based reference management software package (ProQuest LCC, Ann Arbor, MI, USA), and an initial elimination process of duplicates was conducted program-controlled.

Screening Process and Data Extraction
The titles and abstracts of the retrieved references were screened by two independent researchers according to the inclusion and exclusion criteria detailed above, and further duplicates were removed.Non-matching assignments were discussed and, in cases of doubt, decided upon with a third researcher.Full texts were obtained from the remaining references and these were reviewed by the participating researchers following the same procedure.Data were extracted into a pre-designed and pre-tested spreadsheet by one researcher, and supervised and reviewed by a second researcher.Data items to be extracted included, among other things, the database source of the reference, the DOI, and further specific data concerning the publication such as author, title, journal, year of publication, etc., information on study design and participants, year of data collection, and finally, decisive information on the investigation like methodology and devices, purpose, intervention, comparator, outcomes, and key findings.Missing technical information on thermographic devices was searched on the internet where possible and mostly taken from technical descriptions of the specific camera models or, in a few cases, from other studies that used the same camera model.
Based on the extracted data, results are divided with regard to the research topics described above (i.e., medical application areas, purpose of application, technical and environmental modalities, diagnostic performance, reference data/normal values) and presented accordingly in graphical, tabular, and narrative form.

Results
Out of a total of 5027 hits, 72 full texts could be included in the data extraction of the scoping review.Duplicates were automatically removed in refworks (108) and manually excluded (292) in the screening process.Fifty-eight full texts had to be excluded due to the fact that the number of participants was below 25, which was defined as an inclusion/exclusion criteria in the protocol.Twenty-six review articles were identified in the title-abstract scan and the included studies and reference lists were screened accordingly.These reviews each examined one specific use case of thermography, including diabetes, non-contact patient monitoring, breast cancer, systemic sclerosis, ocular surface temperature measurement, atherosclerosis, migraine and headache, thyroid nodules, Raynaud's phenomenon, temporomandibular dysfunction, perforator mapping, back and neck syndromes, paediatric issues, periprosthetic joint infections, Caesarean section, degenerative joint diseases, muscle injuries, and neurological issues.There was no review that dealt with more than one field of application.The exact numbers of each step are depicted in the PRISMA flow-chart in Figure 1.A breakdown of the reasons for exclusions in full-text screening with the respective percentages is shown in Figure 2. A breakdown of the reasons for exclusions in full-text screening with the respective percentages is shown in Figure 2.

Scientific Studies on Medical Thermography: Diseases, Areas of Application, and Primary Study Intentions
To achieve the primary objective of this review, the first step was to create a representative catalogue of all medical use cases of thermography.Ultimately, a total of 38 different indications (close indications have been counted together) have been identified from the extracted data of the included literature sources and are depicted in  Thermography is intended to detect (screen), determine (diagnose), observe, and/or evaluate (monitor), among other things, the state of disease and health.A fourth category specific to this work is the assessment of reference values, i.e., normative data.The different categories are visualized with a pie chart in Figure 3.
2007 [75] rheumatology 41 Thermography is intended to detect (screen), determine (diagnose), observe, and/or evaluate (monitor), among other things, the state of disease and health.A fourth category specific to this work is the assessment of reference values, i.e., normative data.The differ ent categories are visualized with a pie chart in Figure 3.The use of thermography for indications in specific diseases, as shown in Table 3 ranges from acute appendicitis to wound infection.Figure 4 provides an overview of the distribution in the individual therapeutic disciplines.The use of thermography for indications in specific diseases, as shown in Table 3, ranges from acute appendicitis to wound infection.Figure 4 provides an overview of the distribution in the individual therapeutic disciplines.
The included studies were conducted in the Americas, Asia, and Europe, with India, the USA, China, the UK, and Italy being the most frequently represented countries in descending order.Looking at the included references in terms of their number and year of publication, an increase over time is evident with a peak in 2020/21 with 23 publications.The included studies were conducted in the Americas, Asia, and Europe, with the USA, China, the UK, and Italy being the most frequently represented countrie scending order.Looking at the included references in terms of their number and publication, an increase over time is evident with a peak in 2020/21 with 23 public Figure 5 provides an overview of the distribution of publication numbers of the in references summarized in time units of three years.The included studies were conducted in the Americas, Asia, and Europe, with India the USA, China, the UK, and Italy being the most frequently represented countries in de scending order.Looking at the included references in terms of their number and year o publication, an increase over time is evident with a peak in 2020/21 with 23 publications Figure 5 provides an overview of the distribution of publication numbers of the included references summarized in time units of three years.

Thermography Cameras and Technical Properties, Imaging Specifications and Procedures
For the 72 included studies, several thermal imaging camera models from variou manufacturers with different performance parameters were used under specified condi tions.Also, since some articles described the use of multiple models for comparative anal yses, Table 4 lists the information for sixty-four different thermal imagers from fifteen manufacturers and one proprietary system.

Thermography Cameras and Technical Properties, Imaging Specifications and Procedures
For the 72 included studies, several thermal imaging camera models from various manufacturers with different performance parameters were used under specified conditions.Also, since some articles described the use of multiple models for comparative analyses, Table 4 lists the information for sixty-four different thermal imagers from fifteen manufacturers and one proprietary system.
Glaucoma, 2022 [45] Ischaemic heart disease, 2021 [54] OptoTherm, Sewickley, PA, USA Thermoscreen 640 × 480, <0.04 • C ±1 • C Fever, 2010 [37], 2011 [38], 2013 [39] Proprietary system 256 × 256, 0.06 • C at 30 Three studies were excluded from this listing, because either information on the manufacturer or model, or on the technical specifications of the device, was missing [20,26,53].In total, the application of 69 camera models was reported, with FLIR Systems being used in more than half of the investigations.The thermal resolution of all devices ranged from 48 × 47 to 1024 × 678 pixel with a median at 320 × 240 pixel.Thermal sensitivity was between <0.02 • C and 0.5 • C with a median of 0.07 • C. Data for accuracy ranged from ±0.2 • C to ±5 • C with a median of ±2 • C; for eight devices, accuracy was available only as percentages, and for eleven devices no information was given.
Figure 6 provides an overview of the manufacturers of the devices and the respective number of references reporting their application.Since some studies compared several cameras from different manufacturers, the total is more than 72.The degree to which measurement accuracy depends on environmental conditions, equipment performance parameters, patient management before and during the examination, and procedures during imaging was established quite early on.Appropriate and trained personnel, regular testing, the calibration of cameras, and following basic protocols and standard procedures can contribute to the reliability and reproducibility of the results of the technique.Ring and Ammer described factors influencing medical thermography as early as 2000 and called for adherence to guidelines and standards regarding environment, equipment, patient management, imaging procedures, image processing, and image archiving and reporting [86].
Based on the quality assurance guidelines for clinical thermography presented by the International Academy of Clinical Thermology (IACT) in 2015 [87], we have tabulated the key elements that we consider essential to the imaging process.Table 5 lists the relevant requirements reported in the studies included in our scoping review in graded order.Please note that the respective camera specifications are already provided in Table 4.The degree to which measurement accuracy depends on environmental conditions, equipment performance parameters, patient management before and during the examination, and procedures during imaging was established quite early on.Appropriate and trained personnel, regular testing, the calibration of cameras, and following basic protocols and standard procedures can contribute to the reliability and reproducibility of the results of the technique.Ring and Ammer described factors influencing medical thermography as early as 2000 and called for adherence to guidelines and standards regarding environment, equipment, patient management, imaging procedures, image processing, and image archiving and reporting [86].
Based on the quality assurance guidelines for clinical thermography presented by the International Academy of Clinical Thermology (IACT) in 2015 [87], we have tabulated the key elements that we consider essential to the imaging process.Table 5 lists the relevant requirements reported in the studies included in our scoping review in graded order.Please note that the respective camera specifications are already provided in Table 4.

Diagnostic Performance
Medical thermography and its ability to provide actionable, clinically applicable information for diagnosis, screening, and monitoring is central to health care, as physician decisions regarding therapeutic or preventive measures rely on the correctness of the results.The most commonly reported measures of the accuracy of a medical test are sensitivity and specificity.Based on these values, the positive predictive value (PPV) and the negative predictive value (NPV) can be determined using prevalence.Another useful quality criterion is the area under the curve (AUC) with respect to the receiver operating characteristic (ROC), which is a graphical plot of the true positive rate (sensitivity) against the false positive rate (1-specificity).The AUC takes a value between 0 and 1, with a higher value indicating a higher diagnostic ability to distinguish between the two conditions in question, typically "diseased" and "not diseased."Finally, diagnostic accuracy is the proportion of correctly classified true positives and true negatives among all tests results.Thirty-seven (51%) of the seventy-two included studies evaluated the diagnostic ability of medical thermography in the particular use case.Table 6 contains the corresponding information from the relevant publications.Fever, 2010 [37] Screening   7 visualises the respective data on diagnostic quality in the included studies divided into a time period from 2001 to 2016 and a more recent period from 2017 to 2021.

Thermal Reference Values
Comprehensive normative data of the thermal asymmetry of the human body were published as early as 1988 by Uematsu et al. based on thermographically determined temperature differences (∆T's) of 40 paired regions of the body surface of healthy volunteers [90].In contrast to the absolute temperature, which varies between different individuals, these values of thermal asymmetry obtained from anatomically matching homologous regions are extremely stable and reproducible.

Thermal Reference Values
Comprehensive normative data of the thermal asymmetry of the human body were published as early as 1988 by Uematsu et al. based on thermographically determined temperature differences (ΔT's) of 40 paired regions of the body surface of healthy volunteers In our scoping review, we identified six publications on normative data of surface temperatures of different body regions, which we will report chronologically.First, Niu et al. measured skin temperature using infrared thermography in 25 body areas in 57 healthy volunteers aged 24 to 80 years in 2001 and compared the respective mean values between left and right, over and under 60 years, and female and male participants [47].Their results confirm the basic symmetry of the thermoregulatory system in healthy individuals.In 2012, Vardasca et al. studied six regions of upper and lower extremities in 39 healthy male volunteers [48].Upper arms, forearms, hands, thighs, lower legs, and feet were imaged in anterior and posterior view with an infrared camera and examined with regard to their thermal symmetry.The authors conclude that in the regions of interest under consideration, thermal symmetry does not normally deviate by more than 0.5 • C with a standard deviation of no more than 0.3 • C. Gatt et al. studied thermographic distribution patterns of hand, feet, and lower limbs in 63 healthy male and female adults in 2015 and revealed almost identical symmetry in the thermographic patterns of fingers, palms, shins, and soles [49].They detected only minimal differences in temperature between the participants and a consistent pattern in toes and fingers.In search of the optimal site for measuring body core temperature and assessing the corresponding bilateral differences with infrared thermal imaging, Vardasca et al. recruited 206 young participants of both sexes in 2019 [50].Confirming the reliability and reproducibility of their results obtained in relation to the inner canthi of the eye, they emphasize the potential of the related application of infrared imaging for mass screening.In 2020, Matteoli et al. recorded ocular surface temperature in 220 healthy normal participants to obtain reference values stratified by age and sex for a comparison of healthy versus pathologic conditions [51].In order to be able to use ocular surface temperature for diagnostic purposes, they name some caveats, e.g., age and forehead temperature of the patient in question; however, taking these into account, the present reference values can be applied for the investigation of ocular pathologies.Finally, Lubkowska et al. aimed to thermografically determine the distribution and range of surface temperatures of the entire breast, chest, and abdomen in 105 healthy women with normal weight aged 20-40 years [52].Despite inter-individual differences, they did not find statistically significant differences in the distribution of temperature between both sides of the body in women with normal weight, confirming basic symmetry in healthy tissue.Based on their 2021 study, they recommend individual interpretation of thermographic results, taking into account the physiological distribution of temperature in both breasts.

Objectives of Research
The purpose of this scoping review was to investigate and catalogue the use of passive thermography in medicine as currently described in the scientific literature.The results show that thermography is used or is being investigated for its applicability in many different medical specialities.From acute appendicitis to wound care, thermographic imaging and temperature measurement are used for diagnostic, screening, and monitoring purposes.In addition, sets of reference data were created that are necessary for differentiating pathological from healthy conditions and are used to understand how pathological processes alter normal surface temperature.Fundamental for the usability of new measurement methods is the assessment of their diagnostic quality, mostly with regard to the so-called gold standard for the corresponding diagnosis.In the following section, the results of these individual sub-objectives of our scoping review will be discussed in more detail.

Use Cases of Passive Infrared Thermography
Medical thermography enables the identification of minimal temperature differences between symmetrical body regions or between the diseased and healthy state of a region.For example, even a slightly pronounced asymmetric temperature pattern between the left and right breast can be an indication of pathologic development [91], while a slightly decreased temperature in relation to a control region may be an early warning sign of pressure injury [68].Below, we shortly discuss the most recent exemplary studies for each of the identified therapeutic discipline of thermographic use cases.
In the diagnosis of acute appendicitis, as one of the most common indications for emergency surgery, computed tomography is frequently used when medical history, and physical and laboratory examinations are inconclusive, occurring in one-third of suspected cases [14].The harmful consequences of radiation, especially in children, leads to the search for and evaluation of other diagnostic methods [92].Here, thermography offers several distinct advantages over conventional methods in terms of portability, invasiveness, complexity of performance, ionizing radiation, and costs [14].Interestingly, thermography has also been applied in the diagnosis of autism, a disorder of brain development in which one would not necessarily expect temperature changes of the body surface.But by using facial skin temperature to measure emotions, the processing of which is impaired in people with autism spectrum disorder, autistic children can be distinguished from non-autistic children [17].Breast cancer, as the most common cancer in women, is on the rise worldwide and many countries have initiated massive screening efforts based on the fact that an early detection is associated with lower mortality rates [93,94].Women with mammographic dense breast tissue are at higher risk for cancer, but at the same time, the sensitivity of mammography is reduced due to the radiological density, so other examination methods are needed in this case [26].Thermography, approved by the Food and Drug Administration as an additional option for breast cancer screening, offers an easily implementable and cost-effective alternative thanks to new, sophisticated computer-assisted evaluation capabilities [26].Neck pain is considered one of the most common musculoskeletal disorders with a remarkable economic burden and without one definite treatment [95].A negative correlation of thermographically measured skin temperature at the myofascial trigger points with electromyographic activity suggests increased muscle activity at rest.Therefore, studies have recommended clinical interventions that promote muscle relaxation and increase blood flow [29].In the COVID-19 pandemic, it was necessary to conduct rapid screening, especially in critical locations where large numbers of people congregate, to prevent the spread of viruses as much as possible.Here, the use of a portable thermal imaging camera coupled with smartphones to capture thermal images of the back of individuals with and without COVID-19 is reported.A subsequent classification as healthy or diseased was performed using advanced image processing algorithms [30].Again, thermography is shown to be a tool suitable for low-income countries due to its simplicity of use and low cost.Another promising area for the use of medical thermography is diabetes, particu-larly type 2, the extent of which is now described as pandemic, with an estimated one in two cases worldwide going undiagnosed [96].Since middle-and low-income countries show the highest growth rates of type 2 diabetes, simple and cost-effective screening is particularly beneficial in these countries.Thermography of the face and tongue may be used as a basic and non-invasive screening method [33,34].To better understand the pathophysiology of glaucoma, thermal imaging was used to record ocular surface temperature (OST).The detected differences in OST between glaucomatous and healthy eyes support the assumption of an inflammatory process.Thermography could be used to establish clinical biomarkers for glaucoma, the prognostic value of which needs to be determined in further studies [45].OST is also under consideration in the next study, which examines its association with systemic risk factors of cardiovascular and ischaemic heart disease.The results show that OST is significantly higher in different ocular regions in individuals with a history of ischemic heart disease.The authors propose to further investigate this correlation to evaluate its potential utility as a clinical screening test [54].The aim of the next study was to compare a measure of tissue perfusion around the knee, their mottling score, with skin temperature of the same region in patients with septic shock, and their association with survival.Mottling score and skin temperature were not correlated, and neither were they associated with prognosis of day-28 mortality [76].The usefulness of thermography in assessing postoperative inflammation after third molar removal and its correlation with patient-reported swelling in patients receiving either methylprednisolone or placebo was evaluated in the next research.Two days after surgery, thermograms and swelling were recorded, and temperature difference (∆T) between the operated and control side was calculated.There was no statistical significant difference observed in ∆T and the correlation with swelling was low.The authors conclude that thermography seems to lack the sensitivity to be able to detect differences in the inflammatory response with regard to methylprednisolone or placebo [66].A new computer-aided system for assisting diagnosis in rheumatoid arthritis (RA) was tested in women with and without RA.Thermal and RGB images were taken and hand grip strength and gripping force recorded, and an optimal diagnostic model was developed.Interestingly, the RGB delivered better results than the thermal images.However, the images were processed on the basis of their colour, i.e., the temperature distribution in the thermogram was only indirectly taken into account [74].The number of Caesarean sections is increasing globally, and wound care, especially in women with obesity, is challenging in terms of the prevention of surgical site infections [85].In a pilot study, thermography was found to improve the prediction of infections and thus may complement subjective assessment in identifying patients with the highest need for antibiotics [85].This could lead to an improvement in the follow-up of a caesarean section in women with obesity.
In summary, thermography is much more than just an alternative imaging option; above all, it opens up new diagnostic possibilities based on changes in surface temperatures, which were previously not considered in the diagnosis of many diseases.With the capabilities of AI, evaluation options can be improved and facilitated, and diagnostic quality can be increased, a process that is currently emerging in the area of mammography screening, for example [97].Thermography is still in the early stages of evaluating its potential applications in most areas.Time and again, the included publications emphasized the particular value of thermography for middle-and low-income countries, especially because of its ease of use, portability, and cost-effectiveness.

Technical and Environmental Modalities of Thermographic Imaging, Patient Management
The IACT sets various requirements for a minimum standard of the equipment of the imaging system.In terms of cameras used, we identified some of the most important or most commonly reported for our scoping review.With the exception of three publications, all studies provided sufficient information on the manufacturer and model so that most of the missing technical information could be retrieved from the internet.The use of a specific protocol or recognized standard procedures for thermographic image acquisition was reported in 24 of the included studies.Given the importance attributed to factors such as ambient temperature, patient preparation and acclimatization, and camera orientation and distance in thermographic imaging, this means that two-thirds of the studies either did not follow or at least did not report a protocol in this regard.According to the standards and protocols of the IACT, the minimum thermal resolution of 19,200 pixel was not reached by three devices.The required thermal sensitivity below 0.08 • C was reported for 36 devices and an accuracy of ±2 • C/±2% or better for 44 of 69 devices.A total of twenty-eight models failed to meet the IACT standards, including four due to missing data and seven due to both missing and below standard data.
Given the data from this scoping review, the use of a general standard for thermographic imaging has not yet gained acceptance; in any case, little consideration has been given to the existing IACT standard: Only three of the seventy-two publications included in this scoping review indicated that they followed the IACT standard.For another three and seven studies, respectively, the use of published standard infrared imaging procedures or other comparable standard measures was indicated.A further three studies were conducted using ISO standards for temperature assessment or screening guidelines.Ten study authors reported applying their own protocols.With 48 (67%) of the studies, there was no evidence of the use of a standard regarding the performance of thermographic imaging in more than half of the included references.
Nevertheless, there were notes and reports in 49 publications regarding patient management, which is accurately reported as an important component in the IACT quality assurance guidelines.Careful patient management before and during the examination is considered an important criterion for the accuracy of the images.The training or certification of the personnel assigned to perform thermography, including subsequent analysis, is strongly recommended by the IACT, but there is evidence of the reliance on trained personnel in only eight studies.It is possible that the education and training of personnel, as well as the demands on experience with regard to image analysis, are taking a back seat to the ever-increasing technical capabilities of thermal imaging cameras and the subsequent computer-aided image processing and analysis using machine learning [98].It is precisely these constantly improving features of thermographic equipment that make them so interesting for use in middle-and low-income countries, as is emphasized several times in the included publications.Specific imaging procedures such as instructions on patient position and posture during imaging, optimal camera distance, and defining regions of interest in advance were reported in varying degrees of detail in 65% of the included studies.Finally, the design and environmental conditions of the room intended for imaging are part of the quality control recommended by the IACT and should meet the thermodynamic properties required for thermal imaging.In this regard, corresponding information was found in 58 (81%) of the included studies.
In summary, since the IACT guidelines were rarely applied and the reports regarding thermographic technology and procedures in the individual articles were very heterogeneous in terms of information and detail, it must be stated that urgent efforts must be made to apply uniform standards, both for thermography itself and for the reporting of related studies.

Diagnostic Performance of Passive Infrared Thermography
Data on diagnostic performance were reported in only 51% of studies.They were heterogeneous, with large ranges of 38 to 92 points between the lowest and the highest values for the individual measures.More recent data showed a nonsignificant trend toward improved performance; for example, the median sensitivity in studies from 2019 was 92.9 compared with 84.1 in studies from years prior.Data on measures of diagnostic performance depend on the choice of threshold, with study authors placing different emphasis on sensitivity and specificity.
Most data were available on breast cancer (n = 9) and fever measurements (n = 11), with three studies comparing different devices for fever measurement.In some cases, there were noticeable differences between devices, and there was a tendency for improvement in more recent studies.These improvements could be due to technically more enhanced and sophisticated devices but also to advanced methods of image analysis using dedicated software, including artificial intelligence (AI).These results suggest that both breast cancer and fever or body temperature measurements are appropriate areas for future research in thermography.
Breast cancer screening, for which nine studies are available in our scoping review, is a good example of how thermography can be compared with other medical imaging procedures.There are various other screening modalities for breast cancer in particular, with the gold standard currently being X-ray-based mammography.The related sensitivity is reported to be 80%, which drops to 50% or less for young women and women with dense breasts [99].However, the information on the sensitivity of this gold standard is not uncritical and can certainly be questioned, especially in view of whether it refers to prevalence or incidence screening.In any case, with regard to sensitivity, the mean and standard deviation of the nine studies on thermography included here are 83 ± 23%, with a median of 85%.The high value of the standard deviation is mostly due to a sensitivity of 25% in the earliest study from 2011, which may be regarded as an outlier, as the next highest value is 81.6%.In contrast to mammography, there are no restrictions due to high breast density, which is why thermography is sometimes recommended specifically for this subgroup [100].Nevertheless, such comparisons must be viewed with caution, as they are based on different approaches and can only serve as a guide at best.
Thermography is not equally suitable for all applications.On the one hand, in 2020, two studies with the computer-aided analysis of thermograms of the face and tongue showed accuracy rates of 89% and 94%, respectively, in discriminating between diseased and healthy participants, and thus could be used as a basic and non-invasive screening method for type 2 diabetes [33,34].Another successful application was reported in 2021 for the diagnosis of autism, where a sensitivity of 100%, a specificity of 93%, and an accuracy of 96% were achieved by evaluating thermographic images with a customized convolutional neural network (CNN) architecture [17].On the other hand, in 2017, according to the study authors, thermal imaging was not suitable for the diagnosis of peripheral arterial disease (PAD); however, no data on diagnostic accuracy were available in this study, but the authors identified the fundamental question of whether PAD is associated with significantly reduced temperature, as previously supposed [62].In 2010, thermography also seemed to be of little help in predicting progression in herpes zoster, as the affected skin areas showed higher temperatures in about half of the patients and lower temperatures in the other half [65].A randomized controlled trial from 2014 investigated differences in the effect of methylprednisolone versus placebo on postoperative swelling and inflammation after third molar removal, but the authors concluded that thermography was not sufficiently sensitive for detecting differences in inflammatory response [66].Finally, there are also conflicting assessments by study authors regarding the diagnostic performance of thermography, as the example of temporomandibular dysfunction shows.Two studies from 2013 came to results that were not far apart, with an accuracy of 43.3-50.2% in one study, and 56.3% in the other.Despite this similarity, the authors of the first study concluded that thermography is not sufficiently accurate for diagnosis, while the other authors attested a diagnostic usefulness, which shows that perceived applicability in borderline cases is also in the eye of the beholder.[80,81].
In summary, despite the fact that thermography is not optimal in every setting, positive evaluation prevails.The progressive technical development of the recording devices and the possibilities of applying sophisticated methods of artificial intelligence for the evaluation of the images is shown through the trend of increasing diagnostic performance.

Reference Data on the Thermology of Human Skin
Given the fact that comprehensive normative data on the thermal asymmetry of the human body surface were already published by Uematsu in 1988 [90], there is a need, on the one hand, to verify or update these data by current measurements and, on the other hand, to supplement them with normative data of previously unrecorded areas and regions, e.g., ocular surface temperature.However, the authors of the 1988 study argue that, as expected, absolute temperature can vary over time between non-homologous regions and between individuals, but values of temperature difference (∆T) collected from anatomically matched homologous regions are small, highly stable, and reproducible [90].
In principle, Niu et al. repeated Uematsu's study in 2001 with 25, rather than 37, areas and with the further aim of investigating differences between male and female and young and old healthy participants in Taiwan [47].They found that skin temperatures in 11 areas were slightly lower in the elderly than in the young, for example, in the distal parts of the extremities, and that there were differences between the skin temperatures of elderly women and men in certain body regions.Overall, the temperature differences between the measured areas did not exceed 0.5 • C, broadly confirming the findings of Uematsu et al.Another study, conducted by Verdasca et al. in 2012, confirmed the result that the thermal asymmetry does not exceed the value of 0.5 • C with regard to upper and lower extremities in healthy males [48].Three years later, Gatt et al. also found no evidence of physiological asymmetries between hands, feet, and lower limbs in 63 healthy male and female adults, and small differences were only found inter-individual [49].In view of these convincing data on the symmetry of the temperature of contralateral body surfaces, a possibly pathological process cannot be ruled out in the case of measurements deviating from each other, which can, in principle, contribute to finding a diagnosis.
To provide a basis for assessing pathologic processes of the eye, Matteoli et al. collected reference data on ocular surface temperature stratified by age and sex in 2020 [51].They detected significant differences between ocular regions and dependencies on age and forehead skin temperature as well as on environmental factors.The accurate measurement of eye temperature using thermography provides a better understanding of eye physiology and, in addition to classical diagnostic procedures, offers decision support for therapy.Lubkowska and Chudecka reported reference values for the entire breast surface temperature and assessed the thermal symmetry in healthy women in 2021 [52].This study also confirms the thermal symmetry of the contralateral body surface, but the differences here can be higher than the 0.5 • C mentioned above.In a pilot study of 1008 women in India, the cutoff was set at 2.5 • C, higher values were considered abnormal, and >3.0 • C was suspected to be breast cancer [21].Of the 41 women thus identified with ∆T > 3.0 • C, phylloidestumor was detected in one case and breast cancer in 40 women, three of whom had normal mammography.It should be noted that some benign conditions such as infection or inflammation of the breast parenchyma may also alter the temperature distribution and lead to false positive findings [101].Mammography as the current gold standard for breast cancer screening has several limitations, among which is its unsuitability for women with dense breasts, which especially occurs in younger women, and can lead to false-negative results [102].Thermographic screening results, in contrast, are not affected by breast density.
Finally, in terms of mass screening to identify people with fever, another study by Vardasca et al. from 2019 provided good results when measuring the temperature of the inner canthi of the eyes with infrared thermography [50].This result takes on a whole different weight in light of the Corona pandemic, and the demonstrated reliability and reproducibility of this procedure can make a big difference in similar situations.
In summary, our scoping review identified published normative data for several purposes, the first of which was to confirm, in general, the thermal symmetry of the contralateral body regions, indicating possible pathological changes in cases of thermal deviations.Furthermore, the included studies reported reference data of temperature distribution for specific body regions such as the ocular surface and the female breast, and finally determined the best suitable facial region for the most accurate estimation of core body temperature for possible fever mass screening.

Strengths and Limitations
To our knowledge, this is the first scoping review on the application of passive infrared thermography in the medical field.One of the strengths of this work is the adherence to clearly formulated and detailed instructions by experts regarding the JBI methodology used.The protocol-guided execution with predefined objectives and methods guide the knowledge synthesis in a structured and transparent way and minimize the probability of bias.By including diverse sources, a high sensitivity is achieved in the literature search to find as many references as possible to answer the defined research question and thus achieve the research objective in this article.
This review also has some limitations.Due to the very comprehensive research question and the search strategy defined for it, a high number of eligible studies could be identified.However, the limitations to passive thermography and a required group size of at least 25 participants limited the number of studies that were included.The restriction to a group size of at least 25 participants was already specified in the protocol and was based on the expectation of a large number of eligible studies, which would certainly have been associated with a higher workload.It can also be assumed that the additional gain in knowledge would have been comparatively low due to the weaker significance of studies with low statistical power.Thus, it is possible that not all the literature sources that could have been relevant to the aim of this review have been considered and not all application areas, devices, and application modalities, as well as diagnostic performance and reference values for thermography, are included in the results.

Recent Progress in Medical Infrared Thermography and Implications for Further Research
The future prospects and recent progress of infrared thermography are manifold; only a few selected examples will be presented here, for which, there are currently no or only limited satisfactory imaging options.In addition to the contactless, non-invasive, and portable applicability at manageable costs, the rapidly advancing development of infrared cameras on the one hand and artificial intelligence on the other have had a major influence on the growing attractiveness of thermography in recent years.One field in which thermography has been gaining interest and has become one of its most common medical applications, as shown in this review, is the screening of breast cancer.Thermography's diagnostic quality when screening for breast cancer has improved to a level where it can compete with the gold standard mammography, especially in view of the limited sensitivity of mammography in young women and those with dense breast tissue [103].The disadvantages of mammography are well known, which is why an important research task for thermography is in the field of breast cancer screening.Thanks to the affordability of 3D sensors and increasing computing power, various methods for generating 3D thermograms have already been developed.New approaches in efficient data handling and storage enable the generation of large-scale 3D thermograms in real-time [104].These anatomical 3D thermal models can be used, for example, in the prevention, diagnosis, and monitoring of the diabetic foot, but also for gait analysis, with the latter offering a wide range of clinical applications such as the assessment of orthopaedic disabilities, neurological disorders, risk of falls, rehabilitation, and more [105][106][107].Another promising option is the use of infrared thermography technology for all types of skin lesions, particularly skin cancer but also acne, psoriasis, burns, and other skin conditions, where infrared imaging offers a powerful tool for diagnosis, management, and monitoring [108].A final example, which also illustrates the variety of potential applications, is the respiratory monitoring of premature babies, which is considered an important indicator in neonatal intensive care.Medical staff usually measure respiratory rates by counting abdominal and chest movements, as alternative adhesive sensors on the skin can be uncomfortable and even painful.A method that integrates non-contact visual and thermal imaging to estimate respiratory rate could be a major advance for this sensitive area [109].
When new measures are introduced into medicine, users vacillate between rejection and uncritical acceptance [110,111].In the case of thermography, scientific evidence has increased in recent years, opening up many possible fields and modalities of application.However, even in some areas where thermography offers an alternative diagnostic possibility, there may be a possibly unscientific questioning of its goodness due to economic interests.Against this background, it is necessary to evaluate the diagnostic quality in detail, because this is the only way to achieve sufficient evidence for its use.New studies must be designed, conducted, and then reported according to the highest scientific standardsincluding the application of international standards of thermography procedures.Hence, quite apart from many potential applications not covered in this scoping review, scientific rigour and adherence to protocols and internationally recognized standards are the most important implications for future research.

Conclusions
Thermography is still in the early stages of evaluating its potential in most medical areas.This scoping review provides comprehensive information on the application of passive infrared thermography in numerous medical specialities where thermographic data are used for diagnostic, monitoring, and screening purposes.Various investigations have shown that the differences between contralateral body surfaces are subtle and therefore deviations can give hints for underlying pathological changes that should be further investigated.Thanks to the constantly growing technical possibilities of thermal imaging cameras and computer-aided image processing and analysis through artificial intelligence, the application is facilitated and the specific training and further education of personnel is simplified.Therefore, in addition to all the applications presented in this scoping review, thermography should be researched more intensively, especially in the area of mass screening and early diagnosis, as it combines the best prerequisites for this, such as portability, non-invasiveness, and automated evaluation options with low resource consumption at reasonable costs.As our results show, thermography is already being used in low-and middle-income countries as an alternative to expensive or hitherto non-existent screening modalities for non-communicable diseases with high prevalence.
Medicina 2023, 59, x FOR PEER REVIEW 5 of 32 with more than one field of application.The exact numbers of each step are depicted in the PRISMA flow-chart in Figure 1.

Figure 1 .
Figure 1.PRISMA flow chart of literature retrieval and selection process according to Tricco et al. [13].

Figure 1 .
Figure 1.PRISMA flow chart of literature retrieval and selection process according to Tricco et al. [13].

Figure 2 .
Figure 2. Detailed presentation of the reasons for exclusion in the full text screening.

3. 1 .Figure 2 .
Figure 2. Detailed presentation of the reasons for exclusion in the full text screening.

Figure 3 .
Figure 3. Quantitative assignment of the included studies into categories of the purpose of the re spective research.

Figure 3 .
Figure 3. Quantitative assignment of the included studies into categories of the purpose of the respective research.

Figure 5
provides an overview of the distribution of publication numbers of the included references summarized in time units of three years.Medicina 2023, 59, x FOR PEER REVIEW

Figure 4 .
Figure 4. Therapeutic disciplines and respective number of studies with use of thermograph tified in this scoping review.

Figure 5 .
Figure 5. Number of articles included in the scoping review over time using intervals of thre

Figure 4 .
Figure 4. Therapeutic disciplines and respective number of studies with use of thermography identified in this scoping review.

Figure 4 .
Figure 4. Therapeutic disciplines and respective number of studies with use of thermography iden tified in this scoping review.

Figure 5 .
Figure 5. Number of articles included in the scoping review over time using intervals of three years

Figure 5 .
Figure 5. Number of articles included in the scoping review over time using intervals of three years.

Figure 6 .
Figure 6.Overview of manufacturers and frequency of use of the respective thermographic devices they produced.

Figure 6 .
Figure 6.Overview of manufacturers and frequency of use of the respective thermographic devices they produced.

Figure 7
visualises the respective data on diagnostic quality in the included studies divided into a time period from 2001 to 2016 and a more recent period from 2017 to 2021.

Table 2 .
Search strategy in PubMed.

Table 4 .
Manufacturers, camera models with their essential specifications, and clinical conditions for the indication of application, as well as year of publication and respective references.

Table 5 .
Specific measures of imaging procedures reported in the included studies.
Specifications of the examination room (e.g., room without windows, illuminated with neon lights, no sources of thermal energy) n = 19 Standard procedures of infrared imaging [86,89] Instructions for prepara-Instructions of posi-Constant/controlled ambient temperature

Table 5 .
Specific measures of imaging procedures reported in the included studies.
NOTE.ISO International organization for standardization, TR Technical report, TC Technical committee, SC Subcommittee, IEC International electrotechnical commission, ROI Region of interest.

Table 6 .
Diagnostic performance of thermography in various application areas and with different evaluation methods.