Priestia megaterium Metabolism: Isolation, Identification of Naringenin Analogues and Genes Elevated Associated with Nanoparticle Intervention

The impact of gold nanoparticles (AuNPs) on the biosynthetic manipulation of Priestia megaterium metabolism where an existing gene cluster is enhanced to produce and enrich bioactive secondary metabolites has been studied previously. In this research, we aimed to isolate and elucidate the structure of metabolites of compounds 1 and 2 which have been analyzed previously in P. megaterium crude extract. This was achieved through a PREP-ODS C18 column with an HPLC-UV/visible detector. Then, the compounds were subjected to nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS), and Fourier-transform infrared spectroscopy (FT-IR) techniques. Furthermore, bioinformatics and transcriptome analysis were used to examine the gene expression for which the secondary metabolites produced in the presence of AuNPs showed significant enhancement in transcriptomic responses. The metabolites of compounds 1 and 2 were identified as daidzein and genistein, respectively. The real-time polymerase chain reaction (RT-PCR) technique was used to assess the expression of three genes (csoR, CHS, and yjiB) from a panel of selected genes known to be involved in the biosynthesis of the identified secondary metabolites. The expression levels of two genes (csoR and yijB) increased in response to AuNP intervention, whereas CHS was unaffected.


Introduction
Trace metals can function as effectors of metalloregulators or as cofactors of natural product biosynthetic enzymes in Streptomyces [1]. Additionally, they can influence the growth and morphology of Streptomyces. Recently, many metal ions have been studied for their ability to regulate secondary metabolite production in various microbes [2]. These metal ions can be involved in the initiation, activation, control, and inhibition of a variety of microbiological pathways as well as the interactions between soil, plants, and microbes. They are necessary for several metabolic processes, such as the electron transport chain, photosynthesis, and the transport and storage of various metabolites [3]. For example, copper ions can stimulate differentiation and antibiotic synthesis in Streptomyces coelicolor [4]. They can serve as a crucial cofactor in many enzymes and electron transport proteins through their redox activity [5]. As there is a low intracellular demand for Cu + , some microbes express efflux systems or excrete at least one kind of metal-binding

Bacterial Culture of Large-Scale Fermentation with AuNP Intervention
P. megaterium was initially cultured for 72 h at 30 • C on an agar plate containing the following nutrients: 0.4% (w/v) glucose, 0.4% (w/v) yeast extract, 1% (w/v) malt extract, and 2% (w/v) agar in distilled water. Then, a single colony of the grown bacteria was taken from the agar plate and transferred into a 500 mL Erlenmeyer flask containing 300 mL of sterilized seed media without agar at a pH of 7.4, and the flask was incubated on an orbital shaker at 200 rpm for 72 h at 30 • C. The bacteria were further cultivated in the production media containing 10% (v/v) of the previous seed culture: 200 mL cell culture was used in a 2 L flask and incubated with or without 1.5 nM AuNPs at 30 • C in an orbital shaker incubator at 200 rpm. After six days of bacterial growth in the production media, the biomass was harvested as previously reported [21].

Extraction of Crude Extract
The extraction of targeted compounds after AuNP intervention in P. megaterium metabolism were reported previously [22]. Briefly, the biomass of bacterial growth was centrifuged, and the cell-free supernatants were obtained and then extracted with an equal volume of ethyl acetate. The organic layer was collected and then evaporated by a rotary evaporator to obtain the crude metabolite.

Optimization of HPLC Conditions for the Separation and Isolation of Targeted Compounds
Chromatographic separation was conducted using a reverse-phase PREP-ODS C 18 column (250 mm × 4.6 mm, 5 µm) with a Waters®2489 UV/Visible detector and 1525 Waters®binary HPLC pump. The initial concentration of the crude extract was 100 mg/mL. The volume of the sample in each injection was 10 µL, and analysis was carried out in triplicate. The wavelength of the maximum absorbance was determined at 240 nm. The optimal mobile phases contained isocratic elution of 5 mM ammonium acetate/HPLC water (solvent A) and MeOH/MeCN (v/v) in a 4:1 ratio (solvent B) with a total flow rate of 0.2 mL/min. Chromatographic isolation was conducted using a reverse-phase Inertsil ODS-3 Prep C 18 column (250 mm x 10 mm, 5 µm) with a total flow rate of 0.55 mL/min and an injection volume of 50 µL. Compound 1 was collected at 59.73-61.1 min and compound 2 at 66.7-68 min. These isolated compounds were finally dried and used later for compound identification.

Structural Elucidation and Characterization of Isolated Compounds by NMR, FT-IR, and MS
The 1 H and 13 C-NMR spectra were obtained on a Jeol Resonance 500 MHz NMR spectrometer (JNM-ECX500II, Jeol Resonance Inc., Tokyo, Japan). The isolated compounds 1 and 2 were dissolved in deuterated methanol (CD 3 OD). The IR spectrum was recorded in a Nicolet 6700 Fourier-transform infrared spectrophotometer (Thermo Fisher Scientific, Massachusetts, USA) using a KBr disc, and the spectrum was scanned from 500 to 4000 cm −1 .

RNA Extraction
Multiple gene expression was analyzed by RT-PCR for both control and AuNP-treated P. megaterium cultures. The bacteria were grown in an agar plate and the seed media were produced using two cultures: the control culture containing production media with 10% seeded bacteria and the AuNP-treated culture containing production media with 10% seeded bacteria and 1.5 nM AuNPs. The cells were collected by centrifugation from the medium at 24 and 48 h. The RNA was extracted from harvested and fresh cells using an RNeasy®Mini kit according to the instructions included. The concentration of the RNA was determined by a SpectraMax QuickDrop microvolume spectrophotometer (Molecular Devices®).

Reverse Transcriptase PCR
First-strand (single-stranded) cDNA (2 µg) was generated by the reverse transcription of RNA (0.02 µg) with a high-capacity cDNA reverse transcription kit in an ABI Veriti 384-well thermal cycler PCR instrument. RT-PCR was conducted using SYBR™ Green PCR master mix and performed in an Applied Biosystems QuantStudio 3 real-time PCR system.
All of the primers used in our experiments were purchased from Macrogen (Table 1). Primer gyrB was set as the endogenous control [24]. The following cycling parameters were used: 95 • C for 10 min, followed by 40 cycles of 95 • C for 15 s, and 60 • C for 60 s. The QuantStudio 3 program identified the threshold cycle (Ct) for the run based on the fluorescence values that were measured after each cycle. All samples were analyzed in quadruplicate, and the expression of target genes was calculated as relative fold values using the 2 −∆∆Ct method. The expression level of the target genes was normalized to that of gyrB (∆Ct = Ct target-Ct gyrB).

Statistical Analysis
The gene expression test was performed in quadruplicate for the RT-PCR assay and the results were averaged. Data were expressed as the mean ± standard deviation. The data were analyzed using GraphPad Prism 9.0.0. One-way ANOVA was performed for multiple comparisons in this study. Statistical significance was assumed for p-value < 0.05 (* p < 0.05).

Metabolic Profile of AuNP Intervention in P. Megaterium Metabolism
The HPLC profiles of compounds 1 and 2 have previously been studied [22]. However, they were not separated well as the peaks were slightly overlapped. To avoid potential contamination of the compounds, HPLC conditions were further optimized by changing parameters such as UV detection wavelength, column, and mobile phase. As shown in Figure 1, compounds 1 and 2 were separated completely under the optimized conditions. This led to purification of compounds 1 and 2 with a large yield; the yield was 1.3 mg for compound 1 and 1.1 mg for compound 2 per 2 L of P. megaterium culture with AuNP intervention.

Statistical Analysis
The gene expression test was performed in quadruplicate for the RT-PCR assay and the results were averaged. Data were expressed as the mean ± standard deviation. The data were analyzed using GraphPad Prism 9.0.0. One-way ANOVA was performed for multiple comparisons in this study. Statistical significance was assumed for p-value < 0.05 (* p < 0.05).

Metabolic Profile of AuNP Intervention in P. Megaterium Metabolism
The HPLC profiles of compounds 1 and 2 have previously been studied [22]. However, they were not separated well as the peaks were slightly overlapped. To avoid potential contamination of the compounds, HPLC conditions were further optimized by changing parameters such as UV detection wavelength, column, and mobile phase. As shown in Figure 1, compounds 1 and 2 were separated completely under the optimized conditions. This led to purification of compounds 1 and 2 with a large yield; the yield was 1.3 mg for compound 1 and 1.1 mg for compound 2 per 2 L of P. megaterium culture with AuNP intervention.

Isolation of Targeted Compounds
The investigation of secondary metabolites from P. megaterium resulted in the isolation and characterization of two compounds ( Figure 2). The spectroscopic analysis of these compounds was performed using NMR, ESI-MS, and FT-IR techniques. It was found that compounds 1 and 2 were isoflavone derivatives, which are reported for the first time in this bacterium. However, numerous isoflavone derivatives were previously identified in several Streptomyces species [25][26][27][28]. The identity of daidzein (1) and genistein (2) was confirmed by matching with spectroscopic data in the literature [23].

Isolation of Targeted Compounds
The investigation of secondary metabolites from P. megaterium resulted in the isolation and characterization of two compounds ( Figure 2). The spectroscopic analysis of these compounds was performed using NMR, ESI-MS, and FT-IR techniques. It was found that compounds 1 and 2 were isoflavone derivatives, which are reported for the first time in this bacterium. However, numerous isoflavone derivatives were previously identified in several Streptomyces species [25][26][27][28]. The identity of daidzein (1) and genistein (2) was confirmed by matching with spectroscopic data in the literature [23].

Biosynthetic Pathway for the Two Isolated Compounds
We further explored the biosynthetic pathway for the daidzein and genistein isoflavonoids. Both daidzein and genistein are naringenin chalcones derivatives. Flavonoids and isoflavonoids are involved in phenylpropanoid biosynthetic pathways [29]. Phenylpropanoids are exclusively utilized by plants and microorganisms and are derived from the shikimate pathway, which generates essential aromatic amino acids such as phenylalanine and/or tyrosine. The biosynthetic pathway of both daidzein and genistein was already studied in plants [30]. Meanwhile, the entire biosynthesis pathway of naringenin is found in Gram-positive S. calvuligerus [31]. In recent years, researchers have studied the effect of NPs on Streptomyces metabolism to produce antibiotic secondary metabolites, with or without the induction of ROS [10,11,32]. Furthermore, CYP450 could dramatically

Biosynthetic Pathway for the Two Isolated Compounds
We further explored the biosynthetic pathway for the daidzein and genistein isoflavonoids. Both daidzein and genistein are naringenin chalcones derivatives. Flavonoids and isoflavonoids are involved in phenylpropanoid biosynthetic pathways [29]. Phenylpropanoids are exclusively utilized by plants and microorganisms and are derived from the shikimate pathway, which generates essential aromatic amino acids such as phenylalanine and/or tyrosine. The biosynthetic pathway of both daidzein and genistein was already studied in plants [30]. Meanwhile, the entire biosynthesis pathway of naringenin is found in Grampositive S. calvuligerus [31]. In recent years, researchers have studied the effect of NPs on Streptomyces metabolism to produce antibiotic secondary metabolites, with or without the induction of ROS [10,11,32]. Furthermore, CYP450 could dramatically affect microbial metabolism for the syntheis of chalcone derivatives. For instance, chalcone synthase (CHS) was found to be cotranscribed by RppA and RppB proteins in an engineered S. griseus. The induced transcript from the CYP450 RPP enzyme would be read via rppA-rppB genes and, due to this close proximity, CYP450 RPP was found to be involved in the synthesis and modification of flavonoids and isoflavonoids [33]. Meanwhile, quinone-forming oxygenases (MomA) belong to the cupin superfamily and are mainly responsible for the binding of one equivalent metal ion. 3,6,8-tetrahydroxynaphthalene (THN) is biosynthesized by rppA and its neighbor gene CYP450-mel. MomA catalyzes the oxygenation of THN to produce flaviolin [34]. Two decades later, the ncs gene encoding naringenin chalcone synthase was discovered in S. clavuligerus. It is required for the link to the ncyP gene which encodes a CYP450 oxygenase. Both are responsible for the entire synthesis of naringenin [31].
The AntiSMASH prediction has previously been discussed and suggested the naringenin molecule that appeared in the MIBiG comparison of the molecules with structural similarities correlated with those found in S. calvuligerus [22]. Indeed, the HPLC profile of P. megaterium from crude extract isolations showed daidzein (1) and genistein (2) isoflavones as shown in Section 3.2 on structure elucidation. There has never been a reported study of naringenin-related compound production and isolation in P. megaterium. Therefore, it would be worthwhile investigating whether the naringenin pathway and putative gene clusters in P. megaterium are comparable to those found in S. clavuligerus and whether the production of isoflavone metabolites is affected by the presence of AuNPs.
In fact, the CHS enzyme in the T3PKS cluster is well studied in plants and fungi [35]. Plant chalcone isomerase (CHI) plays a role in the production of flavonoids and catalyzes the ring fusion of chalcones, which results in the formation of the classic flavanone structure [36]. The first microbial CHI enzyme was discovered in Eubacterium ramulus; its role is the isomerization of naringenin chalcone to naringenin [37].
We investigated the presence of the CHS enzyme in our identified bacterial isolate (P. megaterium). We discovered a 34.3% pairwise identity of CHS for alpha-pyrone synthesis polyketide synthase, located at contig tig00000037 of the genomic draft compared to the Arabidopsis lyrata subsp. Kamchatica CHI gene, as shown in Figure 3. P. megaterium's genome draft has previously been studied and identified using nanopore technology [22].
Curr. Issues Mol. Biol. 2023, 3, FOR PEER REVIEW 7 In fact, the CHS enzyme in the T3PKS cluster is well studied in plants and fungi [35]. Plant chalcone isomerase (CHI) plays a role in the production of flavonoids and catalyzes the ring fusion of chalcones, which results in the formation of the classic flavanone structure [36]. The first microbial CHI enzyme was discovered in Eubacterium ramulus; its role is the isomerization of naringenin chalcone to naringenin [37].
We investigated the presence of the CHS enzyme in our identified bacterial isolate (P. megaterium). We discovered a 34.3% pairwise identity of CHS for alpha-pyrone synthesis polyketide synthase, located at contig tig00000037 of the genomic draft compared to the Arabidopsis lyrata subsp. Kamchatica CHI gene, as shown in Figure 3. P. megaterium's genome draft has previously been studied and identified using nanopore technology [22]. Then, we were more interested in finding similar genes from the chalcone synthase/stilbene synthase (CHS/STS) family in other species, such as S. griseus, S. calvuligerus, and Saccharopolyspora erythraea. We constructed a phylogenetic tree using Geneious software based on the CHS enzyme (alpha-pyrone synthesis polyketide synthase) present in the T3PKS cluster from contig tig00000037 in comparison to the other three species mentioned earlier and chose to construct a neighbor-joining consensus tree using nucleotide alignment consensus to identify the percentage of gene identity of related genes ( Figure  4). Then, we were more interested in finding similar genes from the chalcone synthase/stilbene synthase (CHS/STS) family in other species, such as S. griseus, S. calvuligerus, and Saccharopolyspora erythraea. We constructed a phylogenetic tree using Geneious software based on the CHS enzyme (alpha-pyrone synthesis polyketide synthase) present in the T3PKS cluster from contig tig00000037 in comparison to the other three species mentioned earlier and chose to construct a neighbor-joining consensus tree using nucleotide alignment consensus to identify the percentage of gene identity of related genes (Figure 4). thase/stilbene synthase (CHS/STS) family in other species, such as S. griseus, S. calvuligerus, and Saccharopolyspora erythraea. We constructed a phylogenetic tree using Geneious software based on the CHS enzyme (alpha-pyrone synthesis polyketide synthase) present in the T3PKS cluster from contig tig00000037 in comparison to the other three species mentioned earlier and chose to construct a neighbor-joining consensus tree using nucleotide alignment consensus to identify the percentage of gene identity of related genes ( Figure  4).  The resulting tree (Figure 4) was a rooted tree with four taxa and seven nodes. Clades diverge at nodes, representing a speciation of gene identity from a common gene ancestor. A node represents a branching point from the common ancestral microbial CHS-related genes of all taxa described on the tree. tig00000037 "Alpha-pyrone synthesis polyketide synthase" represents an outgroup to those suggested to have similar gene characteristic taxa. tig00000037 formed a clade of the ncs gene from S. clavuligerus that is seen in the resulting phylogenetic tree. Another branch of S. griseus and Saccharopolyspora erythraea constructed a clade. All three taxa collectively form a related clade (tig00000037) that contains the CHS-related gene.
Since the CYP450 oxygenase enzyme is essential in producing naringenin analogues and has always been found with the CHS enzyme, we recognized yjiB encoding the cytochrome p450 enzyme in the terpene cluster at contig tig00000007 of the P. megaterium genomic draft. Figure 5 shows a phylogenetic tree of the yjiB gene in comparison to the same species mentioned above.
The resulting tree was constructed against all CYP450-related genes of the four species mentioned above to confirm whether the yjiB gene is very close to CYP450-related genes. The phylogenetic tree ( Figure 5) showed that the tig00000007 "yjiB" gene represents an outgroup to the suggested CYP450-related genes and has 100% identity to a gene characteristic of a reference source for S. griseus to fall into one clade, despite the fact that CHS is closely related to S. calvuligerus.
We then performed a comparative analysis between the four species that contain both enzymes (CHS and CYP450) responsible for phenylpropanoid's secondary metabolite biosynthesis. Our bacterial isolate shared similar gene properties, indicating that alphapyrone synthesis polyketide synthase and YjiB enzymes could be the main genes converting naringenin chalcone to phenylpropanoid secondary metabolite for the biosynthesis of daidzein and genistein. This suggestion indicates our strain could possess the ability to modify/catabolize flavonoids (naringenin) (Figure 6). tig00000037 formed a clade of the ncs gene from S. clavuligerus that is seen in the resulting phylogenetic tree. Another branch of S. griseus and Saccharopolyspora erythraea constructed a clade. All three taxa collectively form a related clade (tig00000037) that contains the CHSrelated gene.
Since the CYP450 oxygenase enzyme is essential in producing naringenin analogues and has always been found with the CHS enzyme, we recognized yjiB encoding the cytochrome p450 enzyme in the terpene cluster at contig tig00000007 of the P. megaterium genomic draft. Figure 5 shows a phylogenetic tree of the yjiB gene in comparison to the same species mentioned above. The resulting tree was constructed against all CYP450-related genes of the four species mentioned above to confirm whether the yjiB gene is very close to CYP450-related genes. The phylogenetic tree ( Figure 5) showed that the tig00000007 "yjiB" gene represents an outgroup to the suggested CYP450-related genes and has 100% identity to a gene characteristic of a reference source for S. griseus to fall into one clade, despite the fact that CHS is closely related to S. calvuligerus. We then performed a comparative analysis between the four species that contain both enzymes (CHS and CYP450) responsible for phenylpropanoid's secondary metabolite biosynthesis. Our bacterial isolate shared similar gene properties, indicating that alpha-pyrone synthesis polyketide synthase and YjiB enzymes could be the main genes converting naringenin chalcone to phenylpropanoid secondary metabolite for the biosynthesis of daidzein and genistein. This suggestion indicates our strain could possess the ability to modify/catabolize flavonoids (naringenin) (Figure 6).

Gene Source Selection from the Genomic Draft of P. megaterium
The copper-sensing transcriptional repressor (CsoR) gene was also present in the terpene cluster of contig tig00000007. The locations of the most crucial genes and the main regulatory gene that have a role in the biosynthesis of secondary metabolites were extracted from Geneious software and are shown in Figure 7. Table 2 refers to each gene and the product name.

Gene Source Selection from the Genomic Draft of P. megaterium
The copper-sensing transcriptional repressor (CsoR) gene was also present in the terpene cluster of contig tig00000007. The locations of the most crucial genes and the main regulatory gene that have a role in the biosynthesis of secondary metabolites were extracted from Geneious software and are shown in Figure 7. Table 2 refers to each gene and the product name.

Gene Source Selection from the Genomic Draft of P. megaterium
The copper-sensing transcriptional repressor (CsoR) gene was also present in the terpene cluster of contig tig00000007. The locations of the most crucial genes and the main regulatory gene that have a role in the biosynthesis of secondary metabolites were extracted from Geneious software and are shown in Figure 7. Table 2 refers to each gene and the product name.   In Table 3, we have listed the copper-related genes found in the genomic draft of P. megaterium, which have been extracted and identified by Geneious software. There are two copper efflux systems: one is a CopZ-like copper metallochaperone protein and the other is a copper-exporting P-type ATPase (CopA). CsoR and two sets of the copper chaperone are well studied in S. lividans [38]. This produced higher amounts of cytochrome (c) oxidases and tyrosinase in the CsoR deletion strain compared with the wild-type in response to copper stress [38]. In addition, both cytochrome (c) oxidases and quinol oxidases belong to the heme-copper family of oxygen reductases [39,40]. Thus, the presence of copper-related genes (qoxB, qoxC, cyoC, and cytochrome P450(BM-1)) is necessary.

Genes Involved in the Biosynthesis Pathway of Naringenin
According to genome mining in our previous study [22], some genes extracted from the annotation of the genomic draft could be involved in the naringenin pathway. Table S1 illustrates the location of genes putatively involved in the formation of the 4-coumaroyl-CoA precursor. Additionally, it shows the annotation of some crucial proteins involved in the biosynthesis of CHS of terpene and T3PKS clusters and defines the gene function and their percentage similarity to other known genes.

The Induction of csoR, CHS, and yjiB Genes by AuNPs
A recent literature search determined that gyrB was the most frequently used reference gene in P. megaterium; this gene was used to validate RT-PCR-based gene expression experiments as a reference gene in this study. Thirty-five genes were predicted to encode naringenin biosynthesis enzymes (Table S1). Three of them (csoR, yijB, and CHS) were experimentally verified and subsequently subjected to evaluation as candidate genes. The fold expression of the control cultures (−24 h/− 48 h) and AuNP-treated cultures (+ 24 h/+ 48 h) demonstrated that the AuNP intervention significantly elevated csoR and yjiB genes in relation to the 2 −∆∆Ct (fold) relative gene expression as seen in Figure 8a,b. The CHS enzyme is comparable to 4-coumarate-CoA ligase, the enzyme responsible for the initial stages of naringenin synthesis. CHS was not affected at either 24 h or 48 h (Figure 8c).

Genes Involved in the Biosynthesis Pathway of Naringenin
According to genome mining in our previous study [22], some genes extracted from the annotation of the genomic draft could be involved in the naringenin pathway. Table  S1 illustrates the location of genes putatively involved in the formation of the 4-coumaroyl-CoA precursor. Additionally, it shows the annotation of some crucial proteins involved in the biosynthesis of CHS of terpene and T3PKS clusters and defines the gene function and their percentage similarity to other known genes.

The Induction of csoR, CHS, and yjiB Genes by AuNPs
A recent literature search determined that gyrB was the most frequently used reference gene in P. megaterium; this gene was used to validate RT-PCR-based gene expression experiments as a reference gene in this study. Thirty-five genes were predicted to encode naringenin biosynthesis enzymes (Table S1). Three of them (csoR, yijB, and CHS) were experimentally verified and subsequently subjected to evaluation as candidate genes. The fold expression of the control cultures (-24 h/-48 h) and AuNP-treated cultures (+ 24 h/+ 48 h) demonstrated that the AuNP intervention significantly elevated csoR and yjiB genes in relation to the 2 -ΔΔCt (fold) relative gene expression as seen in Figure 8a,b. The CHS enzyme is comparable to 4-coumarate-CoA ligase, the enzyme responsible for the initial stages of naringenin synthesis. CHS was not affected at either 24 h or 48 h (Figure 8c). , and CHS (c) by RT-PCR (using gyrB as a reference gene). The * on the error bars indicates significant differences among the treatments (one-way analysis of variance with Tukey's honestly significant difference; p < 0.05, n = 4; "−24h" and "−48h" are control cultures; "+24h" and "+48h" are AuNP intervention cultures).
The expression level of csoR and yijB (the gene encoding cytochrome p450 of P. megaterium) was upregulated, which is closely associated with the contents of daidzein and genistein. These two genes significantly responded to AuNP intervention, while CHS did not. Similar research on AgNP intervention in yeast cells showed that it has a significant impact on the genome and causes differential expression of a number of genes that are sensitive to chemical stimuli, stress, and transport processes, consequently altering the metabolism of yeast cells [9]. The genes involved in copper transport and heat shock were among those most significantly induced in response to the AgNP intervention [9]. , and CHS (c) by RT-PCR (using gyrB as a reference gene). The * on the error bars indicates significant differences among the treatments (one-way analysis of variance with Tukey's honestly significant difference; p < 0.05, n = 4; "−24h" and "−48h" are control cultures; "+24 h" and "+48 h" are AuNP intervention cultures).

Conclusions
The expression level of csoR and yijB (the gene encoding cytochrome p450 of P. megaterium) was upregulated, which is closely associated with the contents of daidzein and genistein. These two genes significantly responded to AuNP intervention, while CHS did not. Similar research on AgNP intervention in yeast cells showed that it has a significant impact on the genome and causes differential expression of a number of genes that are sensitive to chemical stimuli, stress, and transport processes, consequently altering the metabolism of yeast cells [9]. The genes involved in copper transport and heat shock were among those most significantly induced in response to the AgNP intervention [9].

Conclusions
In our previous research, we have studied the effect of AuNPs on genetic manipulation for enhanced secondary metabolism. This study characterized the unknown secondary metabolites isolated from P. megaterium after AuNP treatment and investigated the effects of AuNPs on the transcription of three genes to elucidate the mechanism of daidzein and genistein accumulation. Using a bioinformatics tool, we further investigated their biosynthetic pathway and the presence of important biosynthetic genes. Transcriptomic response analysis revealed that the AuNP intervention in P. megaterium metabolism induced the expression of a specific gene (csoR) that enhanced the expression of another gene (yjiB) along with the same cluster. The study's exploration of AuNP intervention and its impact on gene expression provides insights into potential strategies for manipulating microbial metabolism. The findings could be useful in industries such as pharmaceuticals and biotechnology.