Recently Approved Drugs for Lowering and Controlling Intraocular Pressure to Reduce Vision Loss in Ocular Hypertensive and Glaucoma Patients

Serious vision loss occurs in patients affected by chronically raised intraocular pressure (IOP), a characteristic of many forms of glaucoma where damage to the optic nerve components causes progressive degeneration of retinal and brain neurons involved in visual perception. While many risk factors abound and have been validated for this glaucomatous optic neuropathy (GON), the major one is ocular hypertension (OHT), which results from the accumulation of excess aqueous humor (AQH) fluid in the anterior chamber of the eye. Millions around the world suffer from this asymptomatic and progressive degenerative eye disease. Since clinical evidence has revealed a strong correlation between the reduction in elevated IOP/OHT and GON progression, many drugs, devices, and surgical techniques have been developed to lower and control IOP. The constant quest for new pharmaceuticals and other modalities with superior therapeutic indices has recently yielded health authority-approved novel drugs with unique pharmacological signatures and mechanism(s) of action and AQH drainage microdevices for effectively and durably treating OHT. A unique nitric oxide-donating conjugate of latanoprost, an FP-receptor prostaglandin (PG; latanoprostene bunod), new rho kinase inhibitors (ripasudil; netarsudil), a novel non-PG EP2-receptor-selective agonist (omidenepag isopropyl), and a form of FP-receptor PG in a slow-release intracameral implant (Durysta) represent the additions to the pharmaceutical toolchest to mitigate the ravages of OHT. Despite these advances, early diagnosis of OHT and glaucoma still lags behind and would benefit from further concerted effort and attention.


Introduction
The aim of this review is to present the pharmacological characteristics, in vivo efficacy, and side-effect profiles of drugs that were approved by global health authorities within the last six years to treat ocular hypertension and open-angle glaucoma. Pre-clinical and clinical data will be appraised, and a brief discourse on future prospects will also be presented.
Blindness is undoubtedly the worst human sense-related ailment that a person has to deal with and endure during their lifetime. Even less severe and dire outcomes due to visual impairment can inflict psychological pain due to feelings of helplessness, loneliness, and alienation that cause social disengagement and isolation with a profound loss in quality of life [1][2][3][4]. Indeed, much has been written about the debilitating eye disease    This culminates in a reduction in RGC axonal connections to the brain and, hence, visual impairment. All these detrimental changes occur imperceptibly, and while the degenerative process occurs over decades, untreated ocular hypertension continues to rob the patient of their peripheral vision (see insets of the two people under normal and glaucomatous conditions). At this point, perhaps over half of the original RGCs and their axons have been destroyed, and the patient is on the dangerous path to becoming blind in the affected eye.

Major Methods to Lower and Control IOP/OHT
Three major types of drugs and procedures are utilized today to lower and control IOP. These constitute pharmaceuticals, implantable AQH shunting devices, and surgeries [5][6][7]24,115,[119][120][121][122]. Over the years, several classes of compounds have been discovered, tested, pharmacologically characterized, developed into suitable dosage forms, and approved by various world health authorities to impart efficacious ocular hypotensive activity in animal models and glaucoma patients [5][6][7][23][24][25]115,116]. Such pharmaceuticals include miotics (pilocarpine and carbachol; dosed topically ocularly (t.o.) up to four-times daily), beta-adrenergic receptor antagonists (timolol and betaxolol; dosed t.o. twice daily), carbonic anhydrase inhibitors (dorzolamide and brinzolamide; dosed t.o. twice daily), alpha-2 adrenergic receptor agonists (brimonidine and apraclonidine; dosed t.o. once/twice daily), FP-prostaglandin-receptor agonists (latanoprost, travoprost, tafluprost, bimatoprost; dosed t.o. once daily at night), and fixed-dose combination products (dosed t.o. once/twice daily) ( Figure 4). Much clinical evidence supports the relatively different magnitudes of IOP-lowering achieved by different classes of these drugs, with FP-receptor agonists having the greatest efficacy and duration of action (25-33% IOP reduced over 24 hrs), followed by other drugs such as beta-blockers (18-26% IOP reduced), alpha-2- . The many aspects of damage to the LC result in retinal ganglion cell axon loss, causing reduction in the optic disc tissue and an increase in the cupping (C). This culminates in a reduction in RGC axonal connections to the brain and, hence, visual impairment. All these detrimental changes occur imperceptibly, and while the degenerative process occurs over decades, untreated ocular hypertension continues to rob the patient of their peripheral vision (see insets of the two people under normal and glaucomatous conditions). At this point, perhaps over half of the original RGCs and their axons have been destroyed, and the patient is on the dangerous path to becoming blind in the affected eye.

Major Methods to Lower and Control IOP/OHT
Three major types of drugs and procedures are utilized today to lower and control IOP. These constitute pharmaceuticals, implantable AQH shunting devices, and surgeries [5][6][7]24,115,[119][120][121][122]. Over the years, several classes of compounds have been discovered, tested, pharmacologically characterized, developed into suitable dosage forms, and approved by various world health authorities to impart efficacious ocular hypotensive activity in animal models and glaucoma patients [5][6][7][23][24][25]115,116]. Such pharmaceuticals include miotics (pilocarpine and carbachol; dosed topically ocularly (t.o.) up to four-times daily), beta-adrenergic receptor antagonists (timolol and betaxolol; dosed t.o. twice daily), carbonic anhydrase inhibitors (dorzolamide and brinzolamide; dosed t.o. twice daily), alpha-2 adrenergic receptor agonists (brimonidine and apraclonidine; dosed t.o. once/twice daily), FP-prostaglandin-receptor agonists (latanoprost, travoprost, tafluprost, bimatoprost; dosed t.o. once daily at night), and fixed-dose combination products (dosed t.o. once/twice daily) (Figure 4). Much clinical evidence supports the relatively different magnitudes of IOP-lowering achieved by different classes of these drugs, with FP-receptor agonists having the greatest efficacy and duration of action (25-33% IOP reduced over 24 h), followed by other drugs such as beta-blockers (18-26% IOP reduced), alpha-2-adrenergic agonists (20-25% IOP reduced), muscarinic agonists (20-25% IOP reduced), and carbonic anhydrase inhibitors (15-25% IOP reduced) ( Figure 4). These drugs induce a variety of ocular and sys-temic side effects and, in some cases, have low efficacy or are relatively short-acting. These inadequacies and troublesome off-target activities often lead to patient non-compliance and, in some cases, complete withdrawal from the medications. Due to space limitations and in order to stay focused on the new generation of drugs, the side-effect profiles of the afore-mentioned compounds will not be discussed here but have been reviewed and discussed elsewhere [123][124][125][126][127]. adrenergic agonists (20-25% IOP reduced), muscarinic agonists (20-25% IOP reduced), and carbonic anhydrase inhibitors (15-25% IOP reduced) ( Figure 4). These drugs induce a variety of ocular and systemic side effects and, in some cases, have low efficacy or are relatively short-acting. These inadequacies and troublesome off-target activities often lead to patient non-compliance and, in some cases, complete withdrawal from the medications. Due to space limitations and in order to stay focused on the new generation of drugs, the side-effect profiles of the afore-mentioned compounds will not be discussed here but have been reviewed and discussed elsewhere [123][124][125][126][127]. The schematics illustrate the processes of AQH production and its removal from the anterior chamber of the eye via the conventional IOP-dependent outflow pathway (TM/SC) and the uveoscleral pathway. Furthermore, the mechanism of action of key approved medications to lower IOP by influencing the latter process and pathways is depicted in both (A) and (B). The red minus symbol denotes inhibition and the green plus symbol indicates stimulation or activation.
During the last several years, new drug classes of compounds with different mechanisms of action from the former drugs have emerged with suitable IOP-lowering and duration of action efficacies in animal models with elevated IOPs see in [7,23,24,80,106,115,127]. Some are undergoing clinical investigations in OHT/OAG patients [115,127]. The other means to lower and control IOP have involved the implantation of miniature devices into the AC using minimally invasive surgeries (MIGS) [120,121]. These AQH microshunts and tubes are manufactured from different biocompatible materials, have different designs and sizes, and have varied locations of AQH drainage from the AC [115,120,121]. Similarly, IOP-lowering has been accomplished using either laserinduced or incisional surgical techniques [115,119,122]. Since all of the latter aspects have been discussed in detail in recent papers and reviews [115,119,121], I shall focus only on the recently approved medications for treating OHT/OAG. . The schematics illustrate the processes of AQH production and its removal from the anterior chamber of the eye via the conventional IOP-dependent outflow pathway (TM/SC) and the uveoscleral pathway. Furthermore, the mechanism of action of key approved medications to lower IOP by influencing the latter process and pathways is depicted in both (A) and (B). The red minus symbol denotes inhibition and the green plus symbol indicates stimulation or activation.
During the last several years, new drug classes of compounds with different mechanisms of action from the former drugs have emerged with suitable IOP-lowering and duration of action efficacies in animal models with elevated IOPs see in [7,23,24,80,106,115,127]. Some are undergoing clinical investigations in OHT/OAG patients [115,127]. The other means to lower and control IOP have involved the implantation of miniature devices into the AC using minimally invasive surgeries (MIGS) [120,121]. These AQH microshunts and tubes are manufactured from different biocompatible materials, have different designs and sizes, and have varied locations of AQH drainage from the AC [115,120,121]. Similarly, IOP-lowering has been accomplished using either laser-induced or incisional surgical techniques [115,119,122]. Since all of the latter aspects have been discussed in detail in recent papers and reviews [115,119,121], I shall focus only on the recently approved medications for treating OHT/OAG.

Rho Kinase Inhibitors (Ripasudil and Netarsudil)
Until quite recently, the only approved drugs that specifically targeted the conventional outflow pathway of AQH egress from the AC were muscarinic receptor agonists such as pilocarpine and α2-adrenoceptor agonists such as brimonidine, the latter having a relatively  (Figure 4; [5][6][7][115][116][117][118]126]). Unfortunately, while these compounds lower IOP reasonably well, their significant side effects severely limit their deployment since the advent and clinical utility of FP-receptor agonist drugs to treat OHT/OAG with less or more tolerable ocular side effects [7,115,116]. Therefore, new TM/SC outflow-promoting therapeutics have been sought as potential replacements for pilocarpine and brimonidine, especially since the use of AQH production inhibitors deprives the tissues lining the AC of nutrients, oxygen, and antimicrobial agents that freshly synthesized AQH brings to the AC.
Rho kinases 1 and 2 are ubiquitous enzymes found in most mammalian cells. These rho kinases (ROCKs) have a diverse set of roles, amongst which are cell mobility, proliferation, and cell contraction [128,129]. Mammalian ROCK consists of a catalytic enzymic kinase domain at the N-terminus, a coiled-coil region, and a pleckstrin homology domain, which decreases the enzymic activity of ROCKs via an autoinhibitory intramolecular fold if RhoA-GTP is absent. ROCKs promote increases in intracellular Ca 2+ and induce the formation of stress fibers and focal adhesions by phosphorylating myosin light chain (MLC), which in turn causes actin binding of myosin II, resulting in smooth muscle contraction [128][129][130][131][132][133] ( Figure 5). Furthermore, ROCKs are partially responsible for neurite retraction through phosphorylation of collapsin response mediator protein-2 (CRMP2) and inhibiting its role in stimulating axonal outgrowth [128,129] (Figure 5). In the current context of OHT and retinal degeneration, the ROCK enzyme is upregulated in the ONH of glaucomatous eyes [134]. On the positive side, inhibitors of ROCKs promote relaxation of the smooth muscle-like cells within the TM and SC and thereby help AQH exit from the latter tissues, thus lowering IOP. Additional benefits of ROCK inhibitors involve up-regulation and activation of phosphoinositide 3-kinase, Ser and Thr kinase AKT (protein kinase B), and endothelial nitric oxide synthase within the endothelial-like cells of the TM and SC [128][129][130][131]. Such activity results in the liberation of nitric oxide (NO), which relaxes neighboring TM/SC cells to enhance outflow AQH. Much has been written about the beneficial effects of this crosstalk in the context of treating OHT and OAG using either ROCK inhibitors and/or guanylate cyclase activators [53,54] and NO-donors [55,115,116] that generate intracellular cGMP to relax TM/SC tissues [53,54,115,128,129]. Some examples of the first-generation ROCK inhibitors include fasudil, Y-27632 and HA-135 (see, for example, [128,129]; Figure 5, both insets). These compounds exhibited relatively low binding affinity for recombinant human ROCK-I and ROCK-II using a radiotracer assay with inhibitory constants (IC 50 s) in the 1.6-6.7 µM range, which was reflected in their relatively low IOP-lowering efficacies in rabbit and monkey eyes [129,132,133]. Even though the absolute inhibitory constants of many ROCK inhibitors obtained using recombinant human enzymes and a fluorescence polarization assay and the latter assay differ [129], including those values obtained using human TM cells [129], the rank order of potency of the ROCK inhibitors is fairly well reproduced [127,129].
With the afore-mentioned research knowledge, many researchers have successfully created new chemical ROCK inhibitor structures and shown them to impart effective ocular hypotensive activity in animals [128][129][130][131][132][133] and, in some cases, human subjects [135][136][137][138][139][140][141][142][143][144]. A fairly unique property of most, if not all, ROCK inhibitors is that they reduce IOP in both normotensive and OHT eyes [128][129][130][131][132][133]. Ripasudil (Glanatec; IC 50 = 9 nM) was the first of the ROCK inhibitors to be approved by the health agency in Japan in 2014 for lowering and controlling IOP [135,136]. More recently, the Food and Drug Administration (FDA) granted marketing approval for netarsudil (Rhopressa; IC 50 = 1 nM) [129,137] in the US for the same indication based on extensive clinical data [138][139][140][141][142][143] (Figure 6). These ROCK inhibitors generally reduce IOP in OHT/OAG patients by 16-20% and 20-25%, respectively, following a single t.o. instilled eyedrop of the medications. As discussed above, ROCK inhibitors stimulate AQH outflow through the conventional TM/SC pathway (Figures 4 and 5). However, due to their vasorelaxant activities, they cause intense hyperemia, conjunctival congestion, corneal verticillata, blurred vision, ocular pain, and general eye irritation (Table 1; Figure 6). A recent report of netarsudil causing punctal stenosis and potentially leading to complete punctal closure also requires attention [140]. In order to enhance the overall ocular hypotensive activity in OAG patients, fixed-dose combinations of netarsudil and latanoprost (e.g., Roclatan; 0.02% netarsudil +0.005% latanoprost) have been achieved, resulting in an overall 31-37% lowering of IOP [118,142]. Such high efficacy has been ascribed to not only stimulation of TM/SC and uveoscleral outflow of AQH, but netarsudil apparently also lowers episcleral venous pressure, and its inhibitory activity at the norepinephrine transporter system probably also contributes to the final IOP reduction observed [137,143]. Even though clinical evidence is currently lacking, in vitro and animal studies have provided data in support of the neuroprotective effects of many different structurally diverse ROCK inhibitors [128,129,[144][145][146][147][148][149][150]. If these observations can be replicated in human subjects, ROCK inhibitors have the potential to help directly protect RGCs and perhaps promote neurite extension. Moreover, they may support RGC-axonal elongation and enhance retinal blood flow, potentially offering optic nerve regeneration capabilities in the future [146,147,151]. Such studies are eagerly awaited. With the afore-mentioned research knowledge, many researchers have successfully created new chemical ROCK inhibitor structures and shown them to impart effective ocular hypotensive activity in animals [128][129][130][131][132][133] and, in some cases, human subjects [135][136][137][138][139][140][141][142][143][144]. A fairly unique property of most, if not all, ROCK inhibitors is that they reduce IOP in both normotensive and OHT eyes [128][129][130][131][132][133]. Ripasudil (Glanatec; IC50 = 9 nM) was the first of the ROCK inhibitors to be approved by the health agency in Japan in 2014 for lowering and controlling IOP [135,136]. More recently, the Food and Drug Administration (FDA) granted marketing approval for netarsudil (Rhopressa; IC50 = 1 nM) [129,137] in the US for the same indication based on extensive clinical data [138][139][140][141][142][143] (Figure 6). These ROCK inhibitors generally reduce IOP in OHT/OAG patients by 16-20% and 20-25%, respectively, following a single t.o. instilled eyedrop of the medications. As discussed above, Figure 5. The role of rho kinases in actin fiber contraction, actin depolymerization, and filament stabilization within a TM/SC or CM cell is shown here in a schematic format. The blockage of such processes and events by rho kinase (ROCK) inhibitors such as ripasudil and netarsudil causes the eventual relaxation of the cells. The inset with one yellow star demonstrates the enhancement of fluid outflow from the TM induced by the ROCK inhibitor fasudil in perfused anterior segments of porcine eyes ex vivo. The inset with two yellow stars illustrates the effect of ROCK inhibitor Y-27632 alone or on endothelin-1 (ET-1)-induced contraction of bovine TM cells under in vitro culture conditions, and the changes in F-actin, phosphorylated myosin light chain (ppMLC), and vinculin induced by these compounds. the norepinephrine transporter system probably also contributes to the final IOP reduction observed [137,143]. Even though clinical evidence is currently lacking, in vitro and animal studies have provided data in support of the neuroprotective effects of many different structurally diverse ROCK inhibitors [128,129,[144][145][146][147][148][149][150]. If these observations can be replicated in human subjects, ROCK inhibitors have the potential to help directly protect RGCs and perhaps promote neurite extension. Moreover, they may support RGC-axonal elongation and enhance retinal blood flow, potentially offering optic nerve regeneration capabilities in the future [146,147,151]. Such studies are eagerly awaited.

Conjugate of Latanoprost and NO-Donor (Latanoprostene Bunod)
Nitric oxide (NO) is a well-known gaseous transmitter that is produced by NO synthases acting on L-arginine and is released from many different cell types [152][153][154][155][156][157]. NO serves many roles, including relaxation of smooth muscle or smooth muscle-like cells. There are at least three types of NO synthase present in various mammalian cells. These include the calcium-calmodulin controlled isoenzymes eNOS (endothelial NOS) and nNOS (neuronal NOS) [152][153][154][155][156][157]. The inducible isoform, iNOS, also interacts with calmodulin and produces NO for immune defense purposes to eliminate pathogens. Importantly, eNOS has been localized to human TM, SC, and collecting channels, as well as in the ciliary muscle (CM), especially its anterior longitudinal portion [153]. This part of the CM is connected to the TM via tendinous insertions and modulates TM relaxation/contraction to regulate the outflow resistance. Direct t.o. or intracameral application of NO donor agents has been reported to stimulate TM outflow of AQH, and some animal-based and early clinical investigations have indicated that systemic administration of nitro-vasodilators can lower IOP at doses that do not compromise the cardiovascular system [152,154]. A corollary observation to the former situation is that OAG patients responded better to the NO-donor drugs than those with other major types of glaucoma [154,157]. This may be due to the reduced number of eNOS-and iNOS-containing TM and SC cells in OAG patients, and perhaps also due to genetic defects (single nucleotide polymorphisms in eNOS) in the signaling mechanisms associated with the NO-guanylate cyclase coupling system noted in OAG patients [152][153][154][155][156][157]. Thus, it makes sense that exogenously delivered NO-donating compounds would be useful in patients with OHT and/or OAG. Other evidence supports the important role of NO endogenously in affecting TM and SC cellular activities. Thus, human TM cell volume was decreased by NO-independent soluble guanylate cyclase activators YC-1 and BAY-58-2667, which involved the BK Ca ion channel [53], and this is responsible for some of the IOP reduction observed with these types of compounds [116,127,155,156]. Additionally, endogenous regulation of human SC volume was linked to NO signaling in vitro, which directly influences IOP in vivo [54,115,116,127,155,156]. Due to the existence and success of various fixed-dose combination products used to manage OHT/OAG [117,118,127], and based on the information mentioned above, researchers devised several types of drug conjugates that are held together through hydrolyzable chemical bonds [158]. One such dual-activity conjugate that demonstrated ocular hypotensive activity in animals and human subjects is latanoprostene bunod (LBN; [155,157] ;  Figures 7 and 8A). As shown below, this combined the FP-receptor agonist functionality with an NO-donating molecule, which, when dosed t.o., penetrates the cornea and is broken down to release latanoprost free acid and NO into the AQH, from where they diffuse to reach several different tissues.   Binding of NO to soluble guanylate cyclase generates intracellular cGMP, which activates protein kinase G that uncouples actin-myosin to relax the TM cells, which results in enhanced outflow of AQH from the anterior chamber and thus a reduction in IOP. The activated PKG also reduces the availability of intracellular Ca 2+ by inhibiting the endoplasmic reticular calcium transporter and by inhibiting a range of ion channels that hyperpolarize and relax the TM and /or SC cells to lower IOP. Direct inhibition of rho kinase by PKG is also possible. In the case of non-pigmented ciliary epithelial (NPCE) cells, there is evidence that the NO-cGMP-activated PKG can inhibit Na-K-ATPase to reduce AQH production and help reduce IOP (see the light blue outlined and unfilled arrow). (B) shows the various cellular and intracellular components involved in mediating the effects of FP-receptor agonists within smooth muscle cells of the ciliary muscle, TM, and SC to promote efflux of AQH from the anterior chamber of the eye to lower IOP. Here, the phospholipase C activated upon binding of the FP-agonist hydrolyzes cell membrane phospholipids to generate intracellular inositol phosphates and diacyl glycerol, which in turn elicit intracellular Ca 2+ release and activation of protein kinase C, respectively, leading to enhanced myosin-activated protein kinase (MAPK) activity. Migration and interactions of MAPK with nuclear materials cause the generation and release of promatrix metalloproteinases (MMPs) into the cytoplasm of target cells. The latter are cleaved to liberate and activate MMPs that digest ECM to create or enlarge spaces between CM fibers and around TM to allow AQH outflow via both UVS and TM/SC pathways, thereby lowering IOP. Since cyclooxygenase-2 (COX-2) is also increased, additional prostanoids are generated and released into the extracellular space. These bind to their cognate receptors, and the signal transduction is further amplified (e. g. (B,C)). (C). Numerous other events occur, and endogenous hormones/peptides (e.g., Stanniocalcin-1; vasoactive intestinal peptide (VIP)) are released that also help AQH outflow and stimulate additional IOP-lowering.
Mechanistically, these individual agents then activate the FP-receptor and guanylate cyclase in their target cells, respectively, to induce the many downstream molecular and cellular events that culminate in promoting AQH efflux from the TM/SC and uveoscleral pathways in order to lower IOP. A major constituent of the TM tissue are smooth muscle- Binding of NO to soluble guanylate cyclase generates intracellular cGMP, which activates protein kinase G that uncouples actin-myosin to relax the TM cells, which results in enhanced outflow of AQH from the anterior chamber and thus a reduction in IOP. The activated PKG also reduces the availability of intracellular Ca 2+ by inhibiting the endoplasmic reticular calcium transporter and by inhibiting a range of ion channels that hyperpolarize and relax the TM and /or SC cells to lower IOP. Direct inhibition of rho kinase by PKG is also possible. In the case of non-pigmented ciliary epithelial (NPCE) cells, there is evidence that the NO-cGMP-activated PKG can inhibit Na-K-ATPase to reduce AQH production and help reduce IOP (see the light blue outlined and unfilled arrow). (B) shows the various cellular and intracellular components involved in mediating the effects of FP-receptor agonists within smooth muscle cells of the ciliary muscle, TM, and SC to promote efflux of AQH from the anterior chamber of the eye to lower IOP. Here, the phospholipase C activated upon binding of the FP-agonist hydrolyzes cell membrane phospholipids to generate intracellular inositol phosphates and diacyl glycerol, which in turn elicit intracellular Ca 2+ release and activation of protein kinase C, respectively, leading to enhanced myosin-activated protein kinase (MAPK) activity. Migration and interactions of MAPK with nuclear materials cause the generation and release of pro-matrix metalloproteinases (MMPs) into the cytoplasm of target cells. The latter are cleaved to liberate and activate MMPs that digest ECM to create or enlarge spaces between CM fibers and around TM to allow AQH outflow via both UVS and TM/SC pathways, thereby lowering IOP. Since cyclooxygenase-2 (COX-2) is also increased, additional prostanoids are generated and released into the extracellular space. These bind to their cognate receptors, and the signal transduction is further amplified (e. g. (B,C)). (C). Numerous other events occur, and endogenous hormones/peptides (e.g., Stanniocalcin-1; vasoactive intestinal peptide (VIP)) are released that also help AQH outflow and stimulate additional IOP-lowering.
Mechanistically, these individual agents then activate the FP-receptor and guanylate cyclase in their target cells, respectively, to induce the many downstream molecular and cellular events that culminate in promoting AQH efflux from the TM/SC and uveoscleral pathways in order to lower IOP. A major constituent of the TM tissue are smooth muscle-like cells, which rapidly relax and decrease their cellular volume via K + -channels in response to the NO-guanylate cyclase-cGMP signaling cascade ( Figure 8A; [45,46]). These changes account for the improvement in TM morphology and health, leading to an increase in AQH outflow. Since NO-donor nitro-vasodilator molecules inhibit Na + -K + -ATPase in the ciliary epithelium [159], additional IOP-lowering by reducing AQH formation may be the outcome. The other major contributor to the IOP-lowering actions of LBN [160] is the FP-receptor agonist, latanoprost free acid (LFA), which is generated after LBN is metabolized down to its constituents (Figure 7). LFA engages phospholipase C upon binding to the FP-receptor and triggers the production of the second messengers, inositol trisphosphates (IP 3 ) and diacyl glycerol (DAG), which trigger the release of intracellular Ca 2+ and activation of protein kinase C, respectively, resulting in cellular contraction [7,20,115,116] (Figure 8B,C). Further amplification of this signaling cascade ( Figure 8B,C) occurs via enhancement of gene expression of COX-2 to generate and release endogenous PGs, which can activate EP2 and EP4 receptors to generate cAMP, which causes cellular relaxation. Thus, a series of contraction/relaxation cycles will probably ensue. Additional stimulation of nuclear activity results in the production and release of peptides and hormones such as vasoactive intestinal peptide (VIP) [161] and stanniocalcin-1 [162], respectively. These agents activate their receptors, and other beneficial cellular activities are induced that all culminate in enhancement of AQH outflow via both TM/SC and UVS pathways to lower IOP.
Early animal-based studies demonstrated the good ocular hypotensive efficacy of LBN in normotensive and OHT eyes of rodents (44% drop in IOP 1.  [127,155]. In a dose-finding clinical study using varying concentrations of LBN (0.006% to 0.040%) and latanoprost 0.005%, 0.024% LBN was found to be the optimal concentration of LBN, which was then used in phase-III studies [163][164][165][166][167]. OHT/OAG patients tolerated 0.024% LBN well, and IOP was reduced to a greater extent than that induced by 0.05% timolol at all time points, including in the morning. LBN 0.024% lowered IOP by 22% within the first month of t.o. dosing and achieved a 26% reduction by 1 year from baseline, indicating that durable and sustained IOP control was possible with this medication (Figure 9; [163][164][165][166][167]). The side-effect profile of 0.024% LBN was also acceptable (Figure 10 [163][164][165][166][167]). According to all of these characteristics, LBN received marketing authorization from the FDA in 2017 and represents a novel class of conjugated drugs that offer AQH drainage from the AC through both conventional and unconventional pathways. Based on the potential for NO to inhibit Na + -K + -ATPase, there may be a small contribution to the lowered IOP via inhibition of AQH formation [159].

Sustained Delivery Bimatoprost Implant
Bimatoprost is the amide prodrug of a potent FP-receptor agonist, 17-phenyl PGF 2α , a free acid. Bimatoprost is metabolized by corneal amidases upon t.o. dosing, and the free acid is considered the active moiety that activates the FP-receptor in the CM and TM to induce IOP-lowering in animals and human subjects [168][169][170][171][172]. As described above for other FP-receptor agonist pro-drugs such as latanoprost and travoprost, bimatoprost free acid binds to the FP-receptor to primarily induce UVS outflow and also increases TM/SC outflow of AQH to reduce IOP (see section under LBN; Figure 8A-C). Whether the recent observation of reduced episcleral venous pressure by bimatoprost in dogs [173] can be reproduced in human OHT/OAG patients remains to be determined. However, given the patient compliance issues described above with t.o. medications for OHT/OAG treatment and to provide sustained delivery of the ocular hypotensive drug over several months, an intracameral injection of a bimatoprost implant was approved by the FDA in 2018 to provide protracted IOP reduction with its attendant side effects [174][175][176][177].  Pooled clinical trial data using 10 or 15 µg of bimatoprost implant revealed at least 20%, 25%, 30%, 35%, and 40% IOP decreases at weeks 2, 6, and 12, which, as expected, were greater than those induced by the comparator timolol [177]; Figure 11. The implant appeared to be well tolerated despite inducing a high level of hyperemia, foreign body sensation, eye pain, photophobia, and overall eye irritation during the first two weeks after implantation. These side effects appeared to subside after this time, but the incidence of corneal endothelial cell loss, blepharitis, corneal edema, iritis, dry eye, and iris pigmentation greatly increased after 2 weeks and appeared to continue increasing over time ( Figure 11). Whether physician and patient acceptance of such features of the implant will endure remains to be determined.

Novel Non-Prostaglandin EP2-Prostanoid Receptor Agonist (Omidenepag Isopropyl)
Over two decades have elapsed since the first prostaglandin (PG) FP-receptor agonist, latanoprost pro-drug, was approved for treating OHT/OAG [116]. During this time, several other PG receptor agonists that stimulated IOP-lowering through other prostanoid receptors reached varying levels of pre-clinical discovery / characterization and clinical development. Some examples include the DP and EP2 receptor agonists (AL-6598); [178,179], EP2receptor agonists (PF-04217329; ONO-AE1-259-0; Butaprost) [180][181][182], the EP4-receptor agonist [183], the thromboxane receptor agonist [184], and the dual FP/EP3 receptor agonist (ONO-9054; Sepetaprost; [185]) with ocular hypotensive activities. The most advanced and recently approved EP2-receptor agonist, which is a unique non-prostanoid drug, is omidenepag isopropyl ester (OMDI; 0.002%), which was approved and marketed in Japan in 2018 as Eybelis and approved by the FDA in 2022 and marketed as Omlonti for lowering and controlling IOP [115,116,[186][187][188]. Omidenepag is the free acid active moiety released into the AQH following hydrolysis of OMDI by corneal esterases after t.o. dosing of OMDI [187]. OMD exhibits a high affinity and selectivity for the EP2-receptor and behaves as a full agonist to induce cAMP production within the target cells of tissues of the outflow (TM/SC; CM/sclera) and inflow (ciliary epithelium) pathways [187,188]; Figure 12A,D. Amongst many drugs and drug candidates, 0.002% and 0.01% OMDI exhibited one of the fastest onsets of action in terms of reducing IOP in many mammalian species, including conscious and sedated Cynomolgus monkey eyes, inducing 44-56% IOP reduction in the latter between 1-4 hrs post t.o. dosing [127,187,188] (Figure 13A-D). The mechanism of action in the mildly sedated monkey eyes was shown to be activation of mainly the UVS outflow of AQH, with an additional contribution from the stimulation of the TM/SC outflow pathway [188], Figure 14. A possible influence of OMD on the AQH inflow mechanism cannot be fully ruled out since functional EP2 receptors are also present on non-pigmented ciliary epithelial cells [189,190] in addition to those found on the CM, TM, and SC [191]. Due to the involvement and ability of cAMP produced by EP2 receptor activation by OMD and the potential up-regulation of inducible cyclooxygenase-2 [192] to stimulate production of endogenous PGs [193][194][195], it is likely that a combination of smooth muscle relaxation (ciliary muscle, TM, and perhaps Schlemm's Canal (SC) cells) [196][197][198][199] (Figures 4 and 8C) and MMPs-induced reduction in ECM in and around TM/ ciliary muscle fibers/sclera [191,200] is responsible for the high IOP-lowering efficacy of OMDI ( Figure 13). OMD also directly suppressed mRNAs and proteins associated with tissue growth factor β2 (TGFβ2)-induced ECM deposition [201], and downregulated mRNAs for fibronectin1, collagen-12A1 and collagen-13A1 in 2D-cultures of human TM cells, thereby reducing the ECM components that pathologically cause outflow resistance [202]. Relaxation of the smooth muscle cells associated with ciliary muscle, TM, and SC cells by EP2 agonists ( Figure 12C) [196][197][198][199] also results in enhanced AQH drainage and ocular hypotension [180,181,188,202]. Taken together, the active forms of EP2-receptor agonists, including OMD, appear to powerfully reduce and control IOP in a sustained manner in multiple species ( Figure 13A-D) [115,187]. They accomplish this by recruiting several molecular and cellular components and factors within the eye's anterior chamber tissues (Figures 4 and 8C). Moreover, it seems that EP2 receptor agonists can also provide protection against endoplasmic reticulum stress in TM cells [203] and reduce the potential conversion of smooth muscle TM cells to a fibroblastic phenotype [204], thereby preserving the integrity and functionality of the AQH drainage system. The multiplicity of actions of OMD observed in vitro and in vivo in animal models of OHT bode well for potentially high ocular hypotensive efficacy in human subjects.
Phase-I clinical studies and the respective OMDI pharmacokinetics data indicated a promising safety and tolerability profile and demonstrated no accumulation following 7 days of t.o. dosing and a short half-life in plasma indicative of low systemic exposure [205]. Taken together, three dose-finding studies and a dose-frequency study demonstrated that, of the seven doses of OMDI studied, 0.002% and 0.0025% induced the best IOP-reducing actions, which were similar to those of latanoprost 0.005%. Since OAG and other forms of glaucoma are chronic diseases requiring long-term t.o. dosing to control IOP, the lowest effective dose is preferred to obtain the highest therapeutic index. Thus, 0.002% OMDI was considered the optimal concentration to achieve the desired IOP reduction with a favorable side-effect profile and ocular hypotensive efficacy comparable to latanoprost 0.005% [206]. In phase-III clinical trials of OMDI ophthalmic solution 0.002% ( Figure 15A-C), two studies used patients with glaucoma or OHT (SPECTRUM 3, NCT03691649; and SPECTRUM 4, NCT03691662); two investigations enrolled patients with open-angle glaucoma or OHT (RENGE, NCT02822729; and PEONY, NCT02981446) [207]; one trial comprised a OAG or OHT patient population (AYAME, NCT02623738) [208]; and two trials used patients with OAG or OHT who were non-/poor responders to latanoprost 0.005% (FUJI, NCT02822742; and SPECTRUM 5, NCT03697811) [209]. The clinically beneficial efficacy of OMDI in treating patients with OAG or OHT was confirmed in phase-III trials that demonstrated IOP-lowering non-inferiority to those with latanoprost 0.005% (AYAME; PEONY) or those with timolol 0.5% (SPECTRUM 4; NCT03691662) and sustained IOP reduction alone (SPEC-TRUM 3; RENGE) or in combination with timolol 0.5% (RENGE) (Figure 15A,B).

Sustained Delivery Bimatoprost Implant
Bimatoprost is the amide prodrug of a potent FP-receptor agonist, 17-phenyl PGF2α, a free acid. Bimatoprost is metabolized by corneal amidases upon t.o. dosing, and the free acid is considered the active moiety that activates the FP-receptor in the CM and TM to induce IOP-lowering in animals and human subjects [168][169][170][171][172]. As described above for other FP-receptor agonist pro-drugs such as latanoprost and travoprost, bimatoprost free acid binds to the FP-receptor to primarily induce UVS outflow and also increases TM/SC outflow of AQH to reduce IOP (see section under LBN; Figure 8A-C). Whether the recent observation of reduced episcleral venous pressure by bimatoprost in dogs [173] can be reproduced in human OHT/OAG patients remains to be determined. However, given the patient compliance issues described above with t.o. medications for OHT/OAG treatment and to provide sustained delivery of the ocular hypotensive drug over several months, an intracameral injection of a bimatoprost implant was approved by the FDA in 2018 to provide protracted IOP reduction with its attendant side effects [174][175][176][177].
Pooled clinical trial data using 10 or 15 µg of bimatoprost implant revealed at least 20%, 25%, 30%, 35%, and 40% IOP decreases at weeks 2, 6, and 12, which, as expected, were greater than those induced by the comparator timolol [177]; Figure 11. The implant appeared to be well tolerated despite inducing a high level of hyperemia, foreign body sensation, eye pain, photophobia, and overall eye irritation during the first two weeks after implantation. These side effects appeared to subside after this time, but the incidence of corneal endothelial cell loss, blepharitis, corneal edema, iritis, dry eye, and iris pigmentation greatly increased after 2 weeks and appeared to continue increasing over time (Figure 11). Whether physician and patient acceptance of such features of the implant will endure remains to be determined.

Novel Non-Prostaglandin EP2-Prostanoid Receptor Agonist (Omidenepag Isopropyl)
Over two decades have elapsed since the first prostaglandin (PG) FP-receptor agonist, latanoprost pro-drug, was approved for treating OHT/OAG [116]. During this time, several other PG receptor agonists that stimulated IOP-lowering through other prostanoid receptors reached varying levels of pre-clinical discovery / characterization and clinical  ; (a,b)) and adverse effects (B) induced by intracameral injection of a sustained delivery polymer rod containing 10 µg or 15 µg of bimatoprost are illustrated. The effect of topically instilled timolol (0.05%, dosed twice daily) on the elicitation of adverse events is also presented for comparison (B) within two days and after two days of treatment. Figure 12. The composite in vitro data pertaining to binding of omidenepag isopropyl (OMDI) and its active free acid (OMD) to various prostanoid receptors and sub-types are shown in (A). Since OMD exhibited the highest affinity (lowest binding inhibition constant, K i ) for the EP2 receptors (A) and significantly lower affinities for other receptors, it was deemed to be EP2-receptor-selective compound (B). The relaxation of cat ciliary muscle by various prostanoids and isoproterenol in vitro is shown in (C). Note the high potency of the natural prostaglandin (PGE2) that interacts mainly with EP2 and EP4 receptors. Finally, the ability of OMD to potently (low nanomolar EC 50 values) activate adenylate cyclase, only via the EP2-receptor, to generate intracellular cAMP is depicted in (D).  Figure 13. The ability of topical ocularly-instilled omidenepag isopropyl ester (OMDI; various concentrations; once daily), the non-prostanoid EP2-receptor agonist, to lower IOP in ocular normotensive rabbits (A), dogs (B), Cynomolgus monkey eyes (C), and in ocular hypertensive Cynomolgus monkeys (D) compared with latanoprost (xalatan; 0.005%) and/or timolol (0.05%) is shown. Note that xalatan did not lower IOP to any appreciable extent in ocular normotensive animals, unlike OMDI. In the ocular normotensive (C) and hypertensive monkeys, OMDI (0.001-0.03%) demonstrated either equivalent or greater effectiveness in lowering IOP than latanoprost (xalatan 0.005%). A second dose of OMDI lowered IOP further after the first dose (C) without inducing tachyphylaxis of the IOP-reducing effects of OMDI. The statistical significance changes verses controls (baselines) are represented by the asterisks and crosses (p < 0.05-0.0001). The arrows signify the t.o. dosing of the compound. Figure 13. The ability of topical ocularly-instilled omidenepag isopropyl ester (OMDI; various concentrations; once daily), the non-prostanoid EP2-receptor agonist, to lower IOP in ocular normotensive rabbits (A), dogs (B), Cynomolgus monkey eyes (C), and in ocular hypertensive Cynomolgus monkeys (D) compared with latanoprost (xalatan; 0.005%) and/or timolol (0.05%) is shown. Note that xalatan did not lower IOP to any appreciable extent in ocular normotensive animals, unlike OMDI. In the ocular normotensive (C) and hypertensive monkeys, OMDI (0.001-0.03%) demonstrated either equivalent or greater effectiveness in lowering IOP than latanoprost (xalatan 0.005%). A second dose of OMDI lowered IOP further after the first dose (C) without inducing tachyphylaxis of the IOP-reducing effects of OMDI. The statistical significance changes verses controls (baselines) are represented by the asterisks and crosses (p < 0.05-0.0001). The arrows signify the t.o. dosing of the compound.
Pharmaceuticals 2023, 16, x FOR PEER REVIEW 22 of 41 Figure 14. The mechanism of action of OMDI in terms of its ability to influence ocular AQH dynamics was studied in lightly anesthetized ocular hypertensive monkeys following ocular dosing with a single drop of 0.002% OMDI. The figure shows that OMDI promoted AQH outflow via both the conventional TM/SC and the uveoscleral outflow pathways (B-D) without altering the production of AQH (A), unlike FP-receptor agonists such as latanoprost that uniquely and primarily activate the uveoscleral pathway (D). Asterisk denotes statistically significant verses the vehicle control.
Phase-I clinical studies and the respective OMDI pharmacokinetics data indicated a promising safety and tolerability profile and demonstrated no accumulation following 7 days of t.o. dosing and a short half-life in plasma indicative of low systemic exposure [205]. Taken together, three dose-finding studies and a dose-frequency study demonstrated that, of the seven doses of OMDI studied, 0.002% and 0.0025% induced the best IOP-reducing actions, which were similar to those of latanoprost 0.005%. Since OAG and other forms of glaucoma are chronic diseases requiring long-term t.o. dosing to control IOP, the lowest effective dose is preferred to obtain the highest therapeutic index. Thus, 0.002% OMDI was considered the optimal concentration to achieve the desired IOP reduction with a favorable side-effect profile and ocular hypotensive efficacy comparable to latanoprost 0.005% [206]. In phase-III clinical trials of OMDI ophthalmic solution 0.002% ( Figure 15A-C), two studies used patients with glaucoma or OHT (SPECTRUM 3, NCT03691649; and SPECTRUM 4, NCT03691662); two investigations enrolled patients with open-angle glaucoma or OHT (RENGE, NCT02822729; and PEONY, NCT02981446) [207]; one trial comprised a OAG or OHT patient population (AYAME, NCT02623738) [208]; and two trials used patients with OAG or OHT who were non-/poor responders to latanoprost 0.005% (FUJI, NCT02822742; and SPECTRUM 5, NCT03697811) [209]. The clinically beneficial efficacy of OMDI in treating patients with OAG or OHT was con- Figure 14. The mechanism of action of OMDI in terms of its ability to influence ocular AQH dynamics was studied in lightly anesthetized ocular hypertensive monkeys following ocular dosing with a single drop of 0.002% OMDI. The figure shows that OMDI promoted AQH outflow via both the conventional TM/SC and the uveoscleral outflow pathways (B-D) without altering the production of AQH (A), unlike FP-receptor agonists such as latanoprost that uniquely and primarily activate the uveoscleral pathway (D). Asterisk denotes statistically significant verses the vehicle control.
OMDI as a treatment option for non-/low responders to latanoprost was established in an open-label phase-III investigation (FUJI; NCT02822742) that demonstrated an additional 3 mmHg IOP-lowering in patients after switching to OMDI for 4 weeks from latanoprost 0.005% ( Figure 15C). This additional IOP-reducing action observed with OMDI has also been demonstrated in US patients in the phase-III SPECTRUM 5 trial, with reductions in IOP of the same order as those in Japanese patients in the FUJI study [210]. additional ~3 mmHg IOP-lowering in patients after switching to OMDI for 4 weeks from latanoprost 0.005% ( Figure 15C). This additional IOP-reducing action observed with OMDI has also been demonstrated in US patients in the phase-III SPECTRUM 5 trial, with reductions in IOP of the same order as those in Japanese patients in the FUJI study [210]. Clinical study data comparing the IOP-lowering efficacy of topical ocularly administered OMDI (0.002%; once daily) and latanoprost (0.005%; once daily) in patients with OHT/OAG is shown for treatment spanning 1-4 weeks (A). A closely similar time-course of efficacy is evident between the two different classes of drugs (A). In the inset are shown the chemical structures of OMDI and its free acid (OMD), which have a non-prostanoid structure and bind to EP2 receptors, as compared to latanoprost, which has typical prostaglandin structural features and whose free acid interacts with the FP-receptor. (B) displays the clinical efficacy of OMDI (0.002%, once daily) in lowering and controlling IOP over 1 year when tested alone in OHT/OAG patients with low baseline IOPs (Group 1; ocular normotensives) or in patients with high baseline IOPs (Group 2; ocular hypertensives), and when tested in the latter group of patients in conjunction with timolol (0.05%, twice daily dosing) (Group 3; ocular hypertensives). (C) depicts the change in IOP reduction when OHT/OAG patients who were low responders to latanoprost (0.005%, once daily dosing) were switched over to topical ocular treatment with OMDI (0.002%, once daily dosing) over a 4-week period of study. The SPECTRUM 5 clinical study was an open-label, multicenter study in patients with OAG or OHT who were latanoprost low-/non-responders, i.e., those that did not achieve a ≥15% reduction in IOP from the end of the washout period to the end of the latanoprost run-in period. A total of 107 patients were enrolled and were t.o. treated with OMDI 0.002% once daily in the evening for 3 months, and their IOPs were measured at three timepoints (08:00, 12:00, and 16:00) on their scheduled visits at Week 2, Week 6, and Month 3. The primary efficacy endpoint of this trial was met (i.e., significant change from baseline in mean diurnal IOP at Month 3). OMDI 0.002% t.o. once daily resulted in an early onset of IOP reduction at the first post-baseline visit (Week 2) and a continued, stable IOP-reducing activity throughout the remainder of the trial. OMDI 0.002% was well tolerated; no patients discontinued due to AEs, and no safety concerns of significance were reported. Of the nine cases of conjunctival hyperemia, eight were mild in severity, and only one case of an increase in eyelash pigmentation was noted.
The novel mechanism of action of OMDI (IOP reduction by recruiting both the conventional and uveoscleral outflow pathways) may explain the additional IOP-reducing effect in both the FUJI study in Japanese patients and the SPECTRUM 5 trial in the US-based patient population. Of note was that results from the RENGE trial showed IOP reductions with OMDI of 0.002% achieved in patients with normal IOPs (i.e., baseline IOPs were ≥16 < 22 mmHg), suggesting that OMDI may be effective in patients with normal tension glaucoma (NTG) ( Figure 15B) [211]. Clearly, the higher the baseline IOPs, the greater the efficacy of OMDI 0.002% ( Figure 15B). More recent studies indicated that OMDI may also be therapeutically useful in Japanese patients with secondary glaucomas and primary angle-closure glaucoma (Figure 16; [211][212][213]). This is where a relatively fast onset of action of OMDI may be beneficial, as identified from the monkey studies [127,187,188]. However, these results require confirmation via additional clinical investigations in Japanese patients and in patients of different ethnicities in order to support these initial observations. Nevertheless, OMDI can be combined with other ocular hypotensive drugs approved for treating OHT/OAG. Data from monkey and human studies provide support for such adjunctive therapies [187,207,214] (Figures 13 and 15B).
Of the ocular side effects with OMDI (in the AYAME and FUJI trials), conjunctival hyperemia was the most common, although this was rather mild in the majority of the subjects. Corneal thickening was also noted ( Figure 17). Mild or moderate macular edema/cystoid macular edema was noted in 14 subjects, but all of these happened in pseudophakic patients in the RENGE trial ( Figure 17). These results support the contraindication of OMDI use in pseudophakic patients as noted in the OMDI 0.002% approved drug label in Japan. Prostaglandin-associated periorbitopathy (PAP) eyelid pigmentation, eyelash growth, and DUES have been reported for all t.o. used FP-receptor agonist drugs [116,[124][125][126]215,216] ( Figure 18). However, in comparison with the latter class of drugs, including bimatoprost and travoprost, OMDI 0.002% induced a much lower frequency (0-2%) of PAP symptoms [217][218][219] (Figures 18 and 19). These data suggest that OHT/OAG patients may benefit from switching to treatment with OMDI 0.002% rather than continuing with FP-receptor agonists and being susceptible to the associated appearance-altering AEs. However, the t.o. treatment paradigm for OHT/OAG patients is in the domain of the treating clinician. The RENGE trial data indicated no appearance-altering side effects throughout the year-long trial. Other researchers have also reported similar results when studying the appearance-altering side effects of t.o. OMDI 0.002% or tafluprost in patients with newly diagnosed OAG or OHT ( Figure 18). Thus, in comparison with tafluprost-treated eyes, those that received t.o. OMDI 0.002% exhibited significantly fewer cases of eyelash growth (0% vs. 32%), a lower rate of eyelid pigmentation (0% vs. 4%), and a lower incidence of DUES (2% vs. 12%). Additional results from the deployment of OMDI 0.002% in a post-marketing real-world study of >1800 Japanese patients reported IOP reductions in patients with normal IOP (≤16 mmHg), consistent with the findings of the RENGE trial in patients with NTG [212]. Furthermore, this new study described no cases of PAP symptoms, cystoid macular edema, or uveitis, further strengthening the safety profile of OMDI 0.002%. Therefore, the clinical evidence gathered thus far suggests that OMDI 0.002% is non-inferior to first-line FP-receptor agonists such as latanoprost 0.005% and is a suitable alternative to the latter class of t.o. medications, thereby avoiding the undesirable facial appearance-altering side effects linked to the use of FP-receptor agonists. Various animalbased and clinical studies have also demonstrated that OMDI can be combined with other IOP-lowering drugs to obtain additional efficacy [187,207,208,214] (Figures 13 and 15B). Such adjunctive therapy will prove useful for patients with many types of glaucoma [212]. Furthermore, recent clinical studies involving OMDI 0.002% demonstrated its beneficial ocular hypotensive properties in patients suffering from not only OHT and OAG, but it also lowered IOP significantly in NTG and ACG patients as well over a 4-week trial period ( Figure 16) [127,212,213]. Of the ocular side effects with OMDI (in the AYAME and FUJI trials), conjunctival hyperemia was the most common, although this was rather mild in the majority of the subjects. Corneal thickening was also noted ( Figure 17). Mild or moderate macular edema/cystoid macular edema was noted in 14 subjects, but all of these happened in pseudophakic patients in the RENGE trial ( Figure 17). These results support the contraindication of OMDI use in pseudophakic patients as noted in the OMDI 0.002% approved drug label in Japan. Prostaglandin-associated periorbitopathy (PAP) eyelid pigmentation, eyelash growth, and DUES have been reported for all t.o. used FP-receptor agonist drugs based and clinical studies have also demonstrated that OMDI can be combined with other IOP-lowering drugs to obtain additional efficacy [187,207,208,214] (Figures 13 and 15B). Such adjunctive therapy will prove useful for patients with many types of glaucoma [212]. Furthermore, recent clinical studies involving OMDI 0.002% demonstrated its beneficial ocular hypotensive properties in patients suffering from not only OHT and OAG, but it also lowered IOP significantly in NTG and ACG patients as well over a 4-week trial period ( Figure 16) [127,212,213].     Figure 19. The relative propensity to induce prostanoid-associated periorbitopathy (PAP) symptoms or other ocular and adjacent tissue cosmetic alterations by four different FP-receptor agonists and an EP2-receptor agonist, OMDI, following topical ocular treatment in ocular hypertensive or glaucoma patients is displayed. The overall severity of the changes induced is shown on a relative scale or graded format ranging from Grade 0 to Grade 3.
On the horizon are a number of pharmaceutical agents in clinical trials that are embedded in sustained-release vehicles, including a silicone ring containing bimatoprost, punctal plugs, and contact lenses that release travoprost or latanoprost, and a microshunt that would release travoprost [220]. A novel dual-activity compound expressing FP-and EP3receptor agonist properties that lowers IOP in animals and human subjects, sepetaprost, has advanced to late-stage clinical evaluation [185]. New classes of compounds with adequate IOP-lowering activity and side-effect profiles in animals are also emerging but require investigative new drug-enabling studies or further phases of drug development to permit their evaluation for durable clinical acceptability as new ocular hypotensive drugs [7,115,116,[127][128][129]227,228]. A few examples include an anticonvulsant, analgesic, and anxiolytic medication used to treat epilepsy, neuropathic pain, fibromyalgia, restless leg syndrome, opioid withdrawal, and generalized anxiety disorder that has been repurposed as an IOP-reducing agent (pregabalin [228]), a prostaglandin EP2-receptor agonist [227], an autotaxin/lysophosphatidic acid receptor antagonist [229] and a TRPV-4 antagonist [50,51] (see [127] for more information on these and additional investigative drug candidates).
In addition to the traditional small-molecule drugs to treat OHT/OAG discussed above, new techniques and technologies are emerging that could potentially provide durable IOP reduction without needing medication eyedrops. These include gene-directed therapies involving the use of small interfering ribonucleic acids (siRNAs) that target relevant receptors (e.g., beta-adrenergic [230]), blocking actions of cytokines [231,232], gene-therapy that introduces RNAs for MMPs to clear ECM around the TM cells [233], and the use of gene-editing technology (CRISPR-Cas9) to affect myocilin [234] or aquaporinchannels to modulate AQH dynamics [235].
In view of the fact that many patients with NTG and/or OAG/OHT on maximally accepted therapy still continue to progress towards more severe visual disability. It indicates that IOP is not the only factor that needs to be modified to claim success in managing the degenerative ocular diseases [5][6][7][10][11][12]. Consequently, direct protection of RGCs, the RGC axons, the optic nerve, and brain thalamic and visual cortical neurons is necessary in addition to decreasing and controlling IOP [236][237][238][239][240]. Consequently, many approaches in this realm are at various stages of maturity in terms of demonstrating neuroprotective and/or regenerative activities. Moreover, potentially disruptive long-term treatment paradigms for OHT/OAG and other forms of glaucoma encompass cell-replacement therapies for both the anterior chamber tissues such as the TM [241][242][243] and also for RGCs in the retina [241,244]. Different types of electrical, electromagnetic, ultrasonic, and optogenetic technologies are also emerging that would be of benefit to patients suffering from OHT/OAG and other glaucomatous diseases [245][246][247]. Many aspects of these novel therapeutic modalities have been recently reviewed and discussed and cover the potential therapeutic effects of AQH-derived and/or retinal organoid-derived extracellular vesicles and their cargos, such as miRNAs and trophic factors, and even the use of nutraceutical approaches to curb vision loss [236][237][238][239][240][241][242][243][244][245][246][247][248][249][250]. Likewise, to aid in the early detection and diagnosis of OHT, OAG, and other degenerative retinal and brain diseases, several novel tools are being evaluated and have advanced to different degrees of predictability, reliability, and acceptability by the scientific community [251][252][253][254][255][256]. We hope that many of the latter diagnostic technologies and therapeutic modalities will enter clinical trials and proceed towards health authority approvals in the near future. Such progress should offer more hope for patients suffering from the afore-mentioned ocular degenerative disorders. Researchers in academia and the biopharmaceutical industry need to keep these patient-and people-centric issues in mind as they forge the pathways for discovering and bringing forward new treatments to help these patients with ocular afflictions such as OHT, OAG, and other glaucomas.

Conflicts of Interest:
The author declares no conflict of interest. Author's only aim is to disseminate relevant information to a wide scientific audience in order to educate and bring awareness to eye diseases and to promote further basic and applied research in this arena for the benefit of patients afflicted with ocular and brain diseases.