Synthesis and Properties of Sucrose- and Lactose-Based Aromatic Ester Surfactants as Potential Drugs Permeability Enhancers

The delivery of therapeutics across biological membranes (e.g., mucosal barriers) by avoiding invasive routes (e.g., injection) remains a challenge in the pharmaceutical field. As such, there is the need to discover new compounds that act as drug permeability enhancers with a favorable toxicological profile. A valid alternative is represented by the class of sugar-based ester surfactants. In this study, sucrose and lactose alkyl aromatic and aromatic ester derivatives have been synthesized with the aim to characterize them in terms of their physicochemical properties, structure–property relationship, and cytotoxicity, and to test their ability as permeability enhancer agents across Calu-3 cells. All of the tested surfactants showed no remarkable cytotoxic effect on Calu-3 cells when applied both below and above their critical micelle concentration. Among the explored molecules, lactose p-biphenyl benzoate (URB1420) and sucrose p-phenyl benzoate (URB1481) cause a reversible ~30% decrease in transepithelial electrical resistance (TEER) with the respect to the basal value. The obtained result matches with the increased in vitro permeability coefficients (Papp) calculated for FTIC-dextran across Calu-3 cells in the presence of 4 mM solutions of these surfactants. Overall, this study proposes sucrose- and lactose-based alkyl aromatic and aromatic ester surfactants as novel potential and safe permeation enhancers for pharmaceutical applications.


Introduction
Nowadays, many diseases are managed using therapies that mostly require injectiononly administration. The option to move to a non-invasive delivery route is highly attractive because of the possibility to increase patient compliance, thereby allowing an easier procedure [1]. Drug delivery technologies that respond to this need are scarce, due to the very low permeation of drugs through mucosal surfaces, which usually represent a barrier to the passage of active compounds from the external environment into the systemic circulation [2,3]. Absorption enhancing agents are commonly utilized to increase the mucosal permeation of macromolecules; however, some of them show unacceptable toxicity profiles [4,5]. The finding of innovative absorption enhancers with a favorable toxicological profile that can effectively improve the mucosal absorption of drugs is therefore an urgent need, and their discovery continues among new chemical classes [6][7][8].
Sugar-based surfactants have attracted considerable attention from formulation scientists in the past few years due to their high biocompatibility and biodegradability [9].

Chemistry
Aryl aromatic and aromatic lactose-and sucrose-based esters were designed, synthesized, and explored for the first time for the applications described herein. With regard to the procedures through which the surfactants were obtained, whereas those of lactose derivatives were previously described [21], those of sucrose-based derivatives were carried out by means of a modified Mitsunobu reaction [22,23]. With respect to the latter method, a lower quantity of aromatic acid (1.5 eq.) was used to obtain the monoesterification in position 6 with a reduction in the formation of undesired products.

Thermogravimetric Analysis (TGA) and Differential Thermal Analysis/Scanning (DTA/DSC) Measurements
All TGA traces showed two thermal events related to the weight loss of surfactants ( Figure 1). The first one (≤5% of the initial mass), occurring in the temperature range of 50-125 • C, was related to water desorption of the absorbed or trapped water. The other one (~65-70% of the initial mass), occurring in the temperature range of 200-400 • C, was associated with the thermal degradation of surfactants. DTA/DSC profiles did not display any remarkable endothermic transition at temperatures lower than those at which degradation occurred, suggesting the possible amorphous nature or the simultaneous melting and degradation of the compounds. Overall, all alkyl aromatic analogues of sucrose-and lactose-based surfactants displayed similar thermal properties.

Thermogravimetric Analysis (TGA) and Differential Thermal Analysis/Scan (DTA/DSC) Measurements
All TGA traces showed two thermal events related to the weight loss of surfact ( Figure 1). The first one (≤ 5% of the initial mass), occurring in the temperature rang 50-125 °C, was related to water desorption of the absorbed or trapped water. The o one (~65-70% of the initial mass), occurring in the temperature range of 200-400 °C, associated with the thermal degradation of surfactants. DTA/DSC profiles did not dis any remarkable endothermic transition at temperatures lower than those at which de dation occurred, suggesting the possible amorphous nature or the simultaneous mel and degradation of the compounds. Overall, all alkyl aromatic analogues of sucroselactose-based surfactants displayed similar thermal properties. The black line in all of the plots refers to the "weight" signal, w the gray line in all of the plots refers to the "heat flow" signal. Figure 2a reports the variation of pyrene fluorescence emission (I and III peaks r over concentration for all of the analyzed surfactants. Pyrene is a common fluoresc probe, widely employed to investigate the aggregation state of surfactant in aqueou lution, as its emission properties are strongly influenced by the polarity of the microe ronment. Specifically, a decrease in the ratio between I and III peaks is observed when hydrophobicity increases in the surroundings of pyrene as a result of the aggregatio surfactants into micelles or supramolecular aggregates [26]. All plots show a sigmo decrease in the I and III pyrene peaks over concentration, denoting that all surfactants self-assemble in H2O. As is common for surfactants, the concentration at which aggr Figure 1. TGA and DTA of lactose-based surfactants (URB1419-URB1421) and sucrose-based surfactants (URB1480-URB1482). The black line in all of the plots refers to the "weight" signal, while the gray line in all of the plots refers to the "heat flow" signal. Figure 2a reports the variation of pyrene fluorescence emission (I and III peaks ratio) over concentration for all of the analyzed surfactants. Pyrene is a common fluorescence probe, widely employed to investigate the aggregation state of surfactant in aqueous solution, as its emission properties are strongly influenced by the polarity of the microenvironment. Specifically, a decrease in the ratio between I and III peaks is observed when the hydrophobicity increases in the surroundings of pyrene as a result of the aggregation of surfactants into micelles or supramolecular aggregates [26]. All plots show a sigmoidal decrease in the I and III pyrene peaks over concentration, denoting that all surfactants can self-assemble in H 2 O. As is common for surfactants, the concentration at which aggregation in aqueous solution occurs is strongly dependent on the hydrophilicity/hydrophobicity balance between the surfactant head and tail [27]. Specifically, changes in the hydrophobicity of the surfactant tail exert a marked effect on the self-assembling properties for a surfactant bearing the same polar head [28]. On the contrary, the variation in the sugar head has a minor effect [28]. As such, the sigmoidal profiles in Figure 2a are shifted toward a lower concentration from URB1480-URB1482 for sucrose-based surfactants and URB1419-URB1421 for lactose-based ones. This trend reflects the different hydrophobicity of the molecules related to the presence of a phenyl (URB1480 and URB1419) or a biphenyl  Indeed, the calculated CMC values were higher for the two surfactants bearing a phenyl group (URB1480 and URB1419) than for the others. For those surfactants with a biphenyl group, the elongation of the hydrophobic tail by the insertion of a methylene linker between the phenyl (hydrophobic chain) and sugar (polar head) moieties caused a further decrease in CMC. No marked differences in CMC values were observed between  Indeed, the calculated CMC values were higher for the two surfactants bearing a phenyl group (URB1480 and URB1419) than for the others. For those surfactants with a biphenyl group, the elongation of the hydrophobic tail by the insertion of a methylene linker between the phenyl (hydrophobic chain) and sugar (polar head) moieties caused a further decrease in CMC. No marked differences in CMC values were observed between the sucrose-based and lactose-based series, underlining the less pronounced effect on CMC exerted by the sugar, as previously observed for other sugar-based surfactants [9].

Critical Micelle Concentration (CMC) Measurements
The DLS from the counts analysis confirmed the self-assembling properties of the amphiphiles analyzed as evidenced by the fluorescence measurements. Figure 2b reports the variation in the scattering intensities to the detector (kCps) as a function of the surfactant concentration.
All the plots represented displayed an inflection point, corresponding to CMC, as a sudden increase in the measured counts of the solution occurred when the surfactants started to aggregate into the micelles. As unimers, instead, the hydrodynamic sizes of the surfactant molecules were too small and the scattering properties of the solutions were not markedly different from those of the medium [20,29].

Cytotoxicity-[3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) Cell Viability and Lactate Dehydrogenase (LDH) Assays
All the compounds were tested to evaluate their cytotoxicity profile on Calu-3 cells. The MTT colorimetric assay, based on the reduction of a yellow tetrazolium salt MTT to purple formazan crystals by metabolically active cells, was used to measure the cellular metabolic activity as an indicator of cell viability, proliferation, and cytotoxicity. Indeed, the viable cells contain NAD(P)H-dependent oxidoreductase enzymes, which reduce MTT to formazan [30]. The compounds did not show changes in the cell viability at all of the concentrations tested except for URB1481, which showed a reduction in viability to just above 70% at the highest tested concentration of 4.5 mM, and URB1419, which produced a decrease in viability of not lower than 70% (Figure 3a). Moreover, the LDH release assay, used to assess the level of plasma membrane damage in a cell population, was performed to evaluate the cytotoxicity of the sugar esters. LDH is in fact a stable enzyme found in all cell types and is rapidly released into the cell culture medium following damage to the plasma membrane that occurs after cell damage or death [31]. The results confirmed the high cell compatibility of the studied compounds at the tested concentrations ( Figure 3b).
All the plots represented displayed an inflection point, corresponding to CMC, as a sudden increase in the measured counts of the solution occurred when the surfactants started to aggregate into the micelles. As unimers, instead, the hydrodynamic sizes of the surfactant molecules were too small and the scattering properties of the solutions were not markedly different from those of the medium [20,29].

Cytotoxicity-[3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) Cell Viability and Lactate Dehydrogenase (LDH) Assays
All the compounds were tested to evaluate their cytotoxicity profile on Calu-3 cells. The MTT colorimetric assay, based on the reduction of a yellow tetrazolium salt MTT to purple formazan crystals by metabolically active cells, was used to measure the cellular metabolic activity as an indicator of cell viability, proliferation, and cytotoxicity. Indeed, the viable cells contain NAD(P)H-dependent oxidoreductase enzymes, which reduce MTT to formazan [30]. The compounds did not show changes in the cell viability at all of the concentrations tested except for URB1481, which showed a reduction in viability to just above 70% at the highest tested concentration of 4.5 mM, and URB1419, which produced a decrease in viability of not lower than 70% (Figure 3a). Moreover, the LDH release assay, used to assess the level of plasma membrane damage in a cell population, was performed to evaluate the cytotoxicity of the sugar esters. LDH is in fact a stable enzyme found in all cell types and is rapidly released into the cell culture medium following damage to the plasma membrane that occurs after cell damage or death [31]. The results confirmed the high cell compatibility of the studied compounds at the tested concentrations ( Figure 3b).

TEER Study
TEER studies were performed in Calu-3 cells (Figure 4) to preliminarily evaluate the potential use of the synthesized sugar esters as absorption enhancers. Data showed that URB1420 and URB1481 were the most effective surfactants for decreasing TEER, while URB1419, URB1421, and URB1482 showed a moderate action. URB1480 only poorly affected TEER, suggesting a possible reduced efficacy in tight junction opening or other membrane perturbation mechanisms [32]. TEER studies were performed in Calu-3 cells (Figure 4) to preliminarily evaluate the potential use of the synthesized sugar esters as absorption enhancers. Data showed that URB1420 and URB1481 were the most effective surfactants for decreasing TEER, while URB1419, URB1421, and URB1482 showed a moderate action. URB1480 only poorly affected TEER, suggesting a possible reduced efficacy in tight junction opening or other membrane perturbation mechanisms [32]. The ability of URB1420 and URB1481 to lower TEER more than other synthesized surfactants suggests that the presence of the p-phenyl benzoate moiety as the hydrophobic tail seems to play a role through the permeation enhancing effect. On the contrary, if the p-biphenyl portion is linked to the sugar not directly but through a methylene spacer, as well as when a benzyl group linked to the ester group is present, the molecule decreases its ability to lower TEER. It is plausible to suppose that the presence of a flexible spacer could induce a conformation change in the biphenyl portion, such as to compromise the goodness of the action. The very small difference in the calculated values of HLB and clogP (Table 1) between the phenyl acetate and p-biphenyl acetate sugars does not allow us to think that these parameters can influence the obtained results. With regard to the toxicity profile, URB1420 demonstrated a good balance between safety and efficacy. Interestingly, TEER reversed to the initial value after 24 h with all the tested surfactants at a concentration of 4 mM, suggesting a transient effect on the Calu-3 monolayers and no cellular damage. The results obtained from the MTT assay support the TEER measurements, which should be interpreted carefully to ensure that the changes in TEER are not due to the permeant damage of the cellular membrane integrity. In fact, a transient modulation of the tight junction opening commonly translates into a reversible effect on TEER, while a permanent perturbation of the membrane integrity is evidenced by a non-reversible effect on TEER. However, it should also be noted that a reversible effect on TEER could be a consequence of a mechanism other than the tight junction opening [33,34]. By comparing the effect on TEER of the lactose-based monoesters versus that of the saturated linear derivatives, it should be noted that alkyl aromatic and aromatic lactose monoesters are less effective when used at non-cytotoxic concentrations. Indeed, URB1420 at the concentration of 4 mM induced a decrease in TEER on Calu-3 cells comparable to that of lactose caprate (C10) and lactose palmitate (C12) monoesters, when used at concentrations of 1 mM and 0.054 mM, respectively [20].
As several studies reported in the literature have demonstrated that a TEER decrease is correlated with an increase in FITC dextran flux across Calu-3 monolayers [35][36][37], a The ability of URB1420 and URB1481 to lower TEER more than other synthesized surfactants suggests that the presence of the p-phenyl benzoate moiety as the hydrophobic tail seems to play a role through the permeation enhancing effect. On the contrary, if the p-biphenyl portion is linked to the sugar not directly but through a methylene spacer, as well as when a benzyl group linked to the ester group is present, the molecule decreases its ability to lower TEER. It is plausible to suppose that the presence of a flexible spacer could induce a conformation change in the biphenyl portion, such as to compromise the goodness of the action. The very small difference in the calculated values of HLB and clogP (Table 1) between the phenyl acetate and p-biphenyl acetate sugars does not allow us to think that these parameters can influence the obtained results. With regard to the toxicity profile, URB1420 demonstrated a good balance between safety and efficacy. Interestingly, TEER reversed to the initial value after 24 h with all the tested surfactants at a concentration of 4 mM, suggesting a transient effect on the Calu-3 monolayers and no cellular damage. The results obtained from the MTT assay support the TEER measurements, which should be interpreted carefully to ensure that the changes in TEER are not due to the permeant damage of the cellular membrane integrity. In fact, a transient modulation of the tight junction opening commonly translates into a reversible effect on TEER, while a permanent perturbation of the membrane integrity is evidenced by a non-reversible effect on TEER. However, it should also be noted that a reversible effect on TEER could be a consequence of a mechanism other than the tight junction opening [33,34]. By comparing the effect on TEER of the lactose-based monoesters versus that of the saturated linear derivatives, it should be noted that alkyl aromatic and aromatic lactose monoesters are less effective when used at non-cytotoxic concentrations. Indeed, URB1420 at the concentration of 4 mM induced a decrease in TEER on Calu-3 cells comparable to that of lactose caprate (C10) and lactose palmitate (C12) monoesters, when used at concentrations of 1 mM and 0.054 mM, respectively [20].
As several studies reported in the literature have demonstrated that a TEER decrease is correlated with an increase in FITC dextran flux across Calu-3 monolayers [35][36][37], a macromolecule permeability assay was then performed using FITC-dextran in order to collect some preliminary evidence whether the URB1420 and URB1481 could serve as permeation enhancers.

Permeability Study
The apparent permeability coefficient (Papp) values of Fluorescin isothiocyanate (FITC)-dextran in the absence (control) and in the presence of URB1481 and URB1420 at a concentration of 4 mM was calculated across Calu-3 cell layers ( Figure 5). In accordance with TEER measurements, both surfactants resulted in an increase in FITC-dextran permeation. Comparing the two surfactants, URB1481 showed a more prominent permeation enhancing effect, while URB1420 demonstrated a moderate permeation that enhanced the Pharmaceuticals 2023, 16, 223 7 of 13 effect but with a better safety profile. Further investigation into the permeation enhancer potential of both surfactants on other molecules and mucosal epithelial cells is warranted.

Permeability Study
The apparent permeability coefficient (Papp) values of Fluorescin isothiocyanate (FITC)-dextran in the absence (control) and in the presence of URB1481 and URB1420 at a concentration of 4 mM was calculated across Calu-3 cell layers ( Figure 5). In accordance with TEER measurements, both surfactants resulted in an increase in FITC-dextran permeation. Comparing the two surfactants, URB1481 showed a more prominent permeation enhancing effect, while URB1420 demonstrated a moderate permeation that enhanced the effect but with a better safety profile. Further investigation into the permeation enhancer potential of both surfactants on other molecules and mucosal epithelial cells is warranted.

Synthesis of Sugar-Based Surfactants
The structures of sugar-based aromatic esters ( Figure 6) were unambiguously assessed by MS, 1 H NMR, and 13 13 C NMR spectra were recorded on a Bruker AC 400 or 101 (Bruker, Billerica, MA, USA) spectrometer, respectively, and were evaluated using the TopSpin 2.1 software package; chemical shifts were determined using the central peak of the solvent. Column chromatography purifications were performed under "flash" conditions using Merck 230-400 mesh silica gel (Darmstadt, Germany). Thin layer chromatography was carried out on Merck silica gel 60 F254 plates, which were visualized by exposure to ultraviolet light and to an aqueous solution of ceric ammonium molybdate.

Synthesis of Sugar-Based Surfactants
The structures of sugar-based aromatic esters ( Figure 6) were unambiguously assessed by MS, 1 H NMR, and 13 13 C NMR spectra were recorded on a Bruker AC 400 or 101 (Bruker, Billerica, MA, USA) spectrometer, respectively, and were evaluated using the TopSpin 2.1 software package; chemical shifts were determined using the central peak of the solvent. Column chromatography purifications were performed under "flash" conditions using Merck 230-400 mesh silica gel (Darmstadt, Germany). Thin layer chromatography was carried out on Merck silica gel 60 F254 plates, which were visualized by exposure to ultraviolet light and to an aqueous solution of ceric ammonium molybdate.     acid 1a-c (0.75 mmol), and dry DMF (1.05 mL) were added. After complete dissolution, the mixture was cooled to 0 • C, and DIAD was added (0.256 mL, 1.35 mmol) dropwise, stirred for 24 h at room temperature, and concentrated. Purification of the residue by column chromatography [CH 2 Cl 2 /CH 3 C(O)CH 3 /CH 3 OH/H 2 O 78:10:10:1.5] resulted in 3a-c as solids.

TGA and DTA/DSC Analyses
TGA and DTA/DSC of the surfactants were carried out using a simultaneous the analyzer STA 6000 (Perkin Elmer Inc., Waltham, MA, USA) at a heating rate of 10 °C from 35 °C to 650 °C in a nitrogen environment.

Fluorimetric Analysis
The pyrene emissions in the surfactant solutions at different concentrations (0. mM) were measured using a LS 55 fluorescence spectrometer (PerkinElmer, Waltar, USA) equipped with a thermostatic bath HAAKE C25P (Artisan Scientific Corpora Champaign, IL, USA). For the analyses, three microliters of the pyrene solution in CH (2 μM) were added to each of the aqueous surfactant solutions. The excitation wavele was 334 nm. Ten acquisitions were recorded for each solution in the range of 200-700 at a temperature of 25 °C. The peak intensity I (λ = 372 nm) to III (λ = 384 nm) ratio plotted against the surfactant concentrations. CMC values were calculated from the ce of the sigmoid by fitting the experimental data through the Boltzmann nonlinear reg sion (GraphPad Prism 6 software) according to the following equation:

TGA and DTA/DSC Analyses
TGA and DTA/DSC of the surfactants were carried out using a simultaneous thermal analyzer STA 6000 (Perkin Elmer Inc., Waltham, MA, USA) at a heating rate of 10 • C/min from 35 • C to 650 • C in a nitrogen environment.

Fluorimetric Analysis
The pyrene emissions in the surfactant solutions at different concentrations (0.03-4 mM) were measured using a LS 55 fluorescence spectrometer (PerkinElmer, Waltar, MA, USA) equipped with a thermostatic bath HAAKE C25P (Artisan Scientific Corporation, Champaign, IL, USA). For the analyses, three microliters of the pyrene solution in CH 3 OH (2 µM) were added to each of the aqueous surfactant solutions. The excitation wavelength was 334 nm. Ten acquisitions were recorded for each solution in the range of 200-700 nm at a temperature of 25 • C. The peak intensity I (λ = 372 nm) to III (λ = 384 nm) ratio was plotted against the surfactant concentrations. CMC values were calculated from the center of the sigmoid by fitting the experimental data through the Boltzmann nonlinear regression (GraphPad Prism 6 software) according to the following equation: where the top and bottom are the plateau of the curve and the hill slope is the steepness.

DLS Measurement of the CMC
The scattering intensity to the detector (counts, kCps) was measured for different concentrations (0.03-4 mM) of surfactant solution using a Malvern Zetasizer Nano S (Malvern, Worcestershire, UK). Measurements were performed at 25 • C at a fixed laser position (4.65) and attenuation (11). CMC values were calculated from counts versus concentration plots by fitting the experimental data using the segmental linear regression model (GraphPad Prism 6 software).

Cytotoxicity Study MTT Cell Viability and LDH Release Assays
Calu-3 cells were seeded on 96-well plates at~50,000 cells per well and were incubated to attain at least 80% confluence before the experiment. Prior to the assay, the culture medium was removed and replaced with different concentrations of surfactants (0.03 to 4.5 mM for the fluorescence spectroscopy and DLS analysis) in Hank's balanced salt solution (HBSS). Triton X-100 (1% v/v in HBSS) and HBSS were used as the positive and negative control, respectively. After 3 h of incubation, the plate was centrifuged at 400× g for 5 min. The supernatant was collected for the LDH assay and the cell viability was measured with the MTT assay according to the manufacturers' instructions, with at least three repeats for each sample.

TEER Measurement
Calu-3 cells were seeded on filter inserts Transwell ® at~200,000 cells per insert. The cells were cultured in Dulbecco Modified Eagle Medium (DMEM)-F12 to confluence for around three weeks using an air-liquid culture condition. The culture medium was changed every 48 h. Prior to sample application, the culture medium was replaced with HBSS. Baseline TEER was recorded following 30 min of equilibration in HBSS. Surfactants at a concentration of 4 mM in HBSS were applied to the apical side of the cell monolayers, and HBSS was applied to the basolateral side. The cells were incubated with the samples or HBSS as a control for 3 h. During this period, TEER was measured every 30 min. The samples were then removed and the cells were washed extensively using HBSS. Culture medium was added to the basolateral chamber and a further measurement of TEER was taken at 24 h following sample application to establish TEER reversibility. The change in TEER was reported as a percentage relative to the baseline value.

In Vitro Cell Permeability Studies
Cell monolayers with TEER over 800 Ω were used in these studies to ensure the integrity of the monolayers. FITC-dextran MW 4000 was used as a model macromolecular drug. Prior to sample application, the culture medium was removed, and the cell layers were washed with HBSS. The cells were equilibrated in HBSS for 30 min. Sucrose ester solutions at 4 mM and dextran at a final concentration of 0.5 mg/mL in HBSS were applied to the apical side of the cells. HBSS was added to the basolateral side. A basolateral solution of 100 µL was collected at 30, 60, 90, 120, 150, and 180 min after sample application and replaced with an equivalent volume of fresh HBSS. The amount of dextran permeating the cell monolayers in 3 h was quantified by spectrofluorometric analysis. Dextran permeability is expressed as the apparent permeability coefficient, calculated using the following equation: where P app is the apparent permeability (cm/s), DQ/Dt is the permeability rate (amount of dextran traversing the cell layers over time), A is the diffusion area of the layer (cm 2 ), and C 0 is the apically added dextran concentration [39].

Statistics
All the data presented are the mean ± standard deviation of triplicate measurements and are representative of at least three independent experiments. The two-tailed paired Student's t-test was used for the TEER and permeability studies. The results were considered significant at the level of p < 0.05.

Conclusions
The increasing need for permeation enhancers to deliver therapeutics across biological membranes (e.g., mucosal membranes) is a concern that needs to be addressed in the pharmaceutical and cosmetic fields. This is due to the fact that the difficulty in the permeation across biological membrane is a limitative step that decreases the bioavailability of the active pharmaceutical ingredients. In this work, we explored newly synthesized sucrose-based surfactants with potential application as permeation enhancers, comparing their results with those obtained by linear homologue lactosebased surfactants. The presented molecules show negligible cytotoxicity in vitro on Calu-3 cells at concentrations causing a moderate decrease in TEER (30-40% with the respect to the control) and a slight increase in the P app coefficient. Moreover, in terms of permeability enhancement, URB1481 is the most effective as it induces a three-fold increase in FITC-dextran permeability across Calu-3 cells, despite the fact that its data are non-statistically significant when compared to the control.
Overall, this study provides new insights into the potential pharmaceutical and cosmetic use of aromatic sugar-based surfactants as potential permeation enhancers endowed with a promising cytotoxicity profile. These results are supportive for the development of a new series of sugar-based surfactants as permeation enhancers for applications in different fields.