Antibacterial and Antimycotic Activity of Epilobium angustifolium L. Extracts: A Review

The aim of this work was to provide an overview of available information on the antibacterial and antifungal properties of Epilobium angustifolium extracts. A literature search of Scopus, PubMed/Medline, and Google Scholar for peer-reviewed articles published between January 2000 and June 2023 was undertaken. A total of 23 studies were eligible for inclusion in this review. Significant variation of antimicrobial activity depending on the tested species and strains, type of extract solvent, or plant organs utilized for the extract preparation was found. E. angustifolium extracts were active against both Gram-positive and Gram-negative bacteria and showed antimycotic effects against the fungi of Microsporum canis and Trichophyton tonsurans and the dermatophytes Arthroderma spp. Greater susceptibility of Gram-positive than Gram-negative bacteria to fireweed extracts was found. A strong antibacterial effect was recorded for Staphylococcus aureus, Bacillus cereus, Micrococcus luteus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii including multi-drug resistant strains. E. angustifolium extract might find practical application as an antimicrobial in wound healing, components of cosmetic products for human and animals, or as food preservatives.


Introduction
Recently, the expansion of drug-resistant pathogens has created demand for novel antimicrobials and stimulated the search for natural plant-based compounds as alternatives to synthetics [1][2][3].Plants are a promising source of antimicrobial compounds: tannins, flavonoids, phenolic acids, essential oils, saponins, alkaloids, etc. Particular attention is paid to polyphenols due to the high diversity in their chemical structure and different mechanisms of activity.Therefore, polyphenol-rich species have been studied in search of new antimicrobials.In the last decade, the chemistry and biological activity of Epilobium angustifolium and related species have been studied intensively [4][5][6][7][8].
Epilobium angustifolium L. (fireweed or rosebay willow herb) is a well-known medicinal plant from the Onagraceae family (Figure 1).The species is distributed widely in the temperate zone of North America and Eurasia.Fireweed plants have been traditionally used as a remedy for various conditions including wound healing, infections, skin infections and diseases, colds, urinary problems such as benign prostatic hyperplasia (BPH) or prostatitis, gastric disorders, migraine headaches, and sleeping disorders [5,7,9].Today, Epilobii angustifolii herba (herb) is often used as a component of nutraceuticals, diet supplements, and cosmetic products.Herb and extracts are commercially available for various indications including BPH, skin irritations, gastrointestinal disorders, or even prostate cancer [6].The EMEA monograph on E. angustifolium and E. parviflorum stated that herbs of these species meet the requirements for "traditional use" as teas and infusions with indications for lower urinary tract symptoms related to BPH [10].Efficacy of E. angustifolium in treatment of BPH has not been sufficiently proven.To date, only one clinical trial has been published [11].The results of this randomized double-blind, placebo-controlled trial showed a decrease in the PVR (post-void residual), IPSS (International Prostate Symptom Score), and nocturia after intake of a food supplement containing standardized E. angustifolium extract.It should be stressed that a number of preclinical studies have documented anti-cancer [12][13][14], anti-androgen [15,16], anti-proliferative [17][18][19][20][21], anti-inflammatory [22][23][24], and antioxidant [25][26][27] properties of E. angustifolium extracts.Analgesics [28], anticholinesterase [23], and skin photoprotective activities of fireweed extract were also reported [29].Recently, some studies also showed wound-healing [30] and cosmetic properties of fireweed [31][32][33].
Pharmaceuticals 2023, 16, x FOR PEER REVIEW 2 of 24 various indications including BPH, skin irritations, gastrointestinal disorders, or even prostate cancer [6].The EMEA monograph on E. angustifolium and E. parviflorum stated that herbs of these species meet the requirements for "traditional use" as teas and infusions with indications for lower urinary tract symptoms related to BPH [10].Efficacy of E. angustifolium in treatment of BPH has not been sufficiently proven.To date, only one clinical trial has been published [11].The results of this randomized double-blind, placebocontrolled trial showed a decrease in the PVR (post-void residual), IPSS (International Prostate Symptom Score), and nocturia after intake of a food supplement containing standardized E. angustifolium extract.It should be stressed that a number of preclinical studies have documented anti-cancer [12][13][14], anti-androgen [15,16], anti-proliferative [17][18][19][20][21], anti-inflammatory [22][23][24], and antioxidant [25][26][27] properties of E. angustifolium extracts.Analgesics [28], anticholinesterase [23], and skin photoprotective activities of fireweed extract were also reported [29].Recently, some studies also showed wound-healing [30] and cosmetic properties of fireweed [31][32][33].The wide spectrum of biological activity of E. angustifolium extracts results from their complex and diverse chemical composition.More than 250 compounds have been identified, including: ellagitannins (hydrolysable tannins), flavonoids, phenolic acids, lignans, steroids, triterpenoids, fatty acids, essential oil, and alkaloids [7].Medicinal properties of E. angustifolium were attributed to the synergic interactions of polyphenols and the high concentration of oenothein B-a macrocyclic (dimer) ellagitannin.Therefore, oenothein B and quercetin-3-O-glucuronide (flavonoid) have been proposed as marker compounds for standardization of the raw material [34].Oenothein B is the most abundant ellagitannin in fireweed plants.This compound represents about 4-8% of the dry mass of herb depending on season, harvest time, plant organ, or genotype [35][36][37][38].Oenothein B showed a broad spectrum of pharmacological properties including antioxidant, anti-cancer, antiandrogen, immunostimulatory, metal binding, and antimicrobial activities [39][40][41].Apart from oenothein B, other ellagitannins from monomeric up to heptamers (e.g., tellimagrandin I, II, woodfordin, oenothein A, and others) were identified in E. angustifolium plants [42].Tannins are known for their antibacterial properties because they react The wide spectrum of biological activity of E. angustifolium extracts results from their complex and diverse chemical composition.More than 250 compounds have been identified, including: ellagitannins (hydrolysable tannins), flavonoids, phenolic acids, lignans, steroids, triterpenoids, fatty acids, essential oil, and alkaloids [7].Medicinal properties of E. angustifolium were attributed to the synergic interactions of polyphenols and the high concentration of oenothein B-a macrocyclic (dimer) ellagitannin.Therefore, oenothein B and quercetin-3-O-glucuronide (flavonoid) have been proposed as marker compounds for standardization of the raw material [34].Oenothein B is the most abundant ellagitannin in fireweed plants.This compound represents about 4-8% of the dry mass of herb depending on season, harvest time, plant organ, or genotype [35][36][37][38].Oenothein B showed a broad spectrum of pharmacological properties including antioxidant, anti-cancer, anti-androgen, immunostimulatory, metal binding, and antimicrobial activities [39-41].Apart from oenothein B, other ellagitannins from monomeric up to heptamers (e.g., tellimagrandin I, II, woodfordin, oenothein A, and others) were identified in E. angustifolium plants [42].Tannins are known for their antibacterial properties because they react irreversibly with membrane proteins, neutralizing bacteria [43].Several different mechanisms of action have been proposed for the antibacterial activity of tannins, such as: inhibition of extracellular microbial enzymes, oxidative phosphorylation, and disruption of cellular membrane permeability.Tannins bind to proteins through non-covalent bonds, leading to the morphological and structural changes and consequently to damages of the membrane integrity.Due to the diversity in the chemical structure of the compounds in this class, potentially each of them possesses antimicrobial properties.
Phenolic acids are significant components of therapeutic activity of E. angustifolium extracts.Gallic, caffeic, ellagic, ferulic, and protocatechuic acids as well as caffeoylquinic acid isomers and others have been found [7,37].Gallic acid was identified as the principal compound responsible for the antioxidant and therapeutic effect against BPH [16,26].Phenolic acids have shown a wide range of biological activities including antibacterial, antiparasitic, and antiviral properties [48,49].Therefore, these substances might affect or modulate the antimicrobial effect of extracts.
Essential oil constituents are known for their antimicrobial activity.The biological activity of the essential oil of E. angustifolium has not been thoroughly studied, but antioxidant, antibacterial, and antimycotic properties have been documented [50][51][52].Other groups of active metabolites such as alkaloids (angustifoline A), lignans, fatty acids (tricosanoic, nervonic, linoleic, palmitic, caprylic, caproic, butyric, and others), and sterols (campesterol, stigmasterol, β-sitosterol, cholesterol, and their derivatives) have also been identified [13,53,54] To date, E. angustifolium extracts have been tested in the treatment of BPH, in wound healing or as ingredients of cosmetic products, nano-bactericides [55], and as a food preservative [56].Antimicrobial properties of fireweed have been studied since the beginning of this century, but in the last decade, the number of publications has increased significantly.However, the data are incomplete and scattered, so a new, comprehensive summary is needed.
The aim of this study was to provide an overview of available information on the antibacterial and antifungal properties of E. angustifolium extracts.This work reviews the current state of knowledge and discusses antimicrobial activity and the relationship between the phytochemical composition of extracts.
Generally, a weak or moderate effect of seed extract was found [61].Comparatively tested leaves and flower extracts demonstrated similarly weak activity against E. coli and P. aeruginosa, but a flower extract showed a stronger inhibitory effect against S. aureus (11 mm vs. 17 mm) and C. albicans (15 mm vs. 20 mm) [60].Kosalec et al. 2013 obtained similar and consistent results [64].Both extracts (leaves and flowers) were active against Gram-positive bacteria, but leaf extract had a significantly lower MIC value (9.1 vs. 16.2 mg/mL) against the C. albicans strain.The differences in the extracts' activity might have resulted from their different chemical compositions.Diversity in the organ distribution of the main phenolic compounds in flowers and leaves of E. angustifolium was found by Baert et al. 2017 [36].Hexameric and heptameric ellagitannins were 3-4 times more abundant in flowers than in leaves.Flavonoids such as quercetin-3-O-rhamnoside, myricetin-3-O-rhamnoside, and kaempferol-3-O-rhamnoside were specific to flower tissue and were absent from leaves.Therefore, the different compositions of ellagitannins and flavonoids in flowers and leaves might be responsible for the varied antimicrobial effect.
The other crucial factor is the solvent used for extraction [81][82][83].Polarity of the solvent is a principal issue due to the different solubility of the various classes of antimicrobial compounds in the solvents [84].Decreasing antimicrobial activity of methanolic > ethanolic > hexanoic > water extract against E. coli, S. aureus, K. pneumoniae, P. aeruginosa was reported by Güven at al. 2020 [66].In another study, a dichloromethane extract containing non-polar compounds showed no or little activity, but methanolic and ethanolic extracts were both active [75].Alcoholic extracts are typically rich in medium-polar compounds such as tannins and flavonoids.Good antimicrobial activity of tannins and flavonoids has been documented [85,86] and supports the obtained results.On the other hand, despite quantitative and qualitative differences in flavonoid composition and phenolic acid content in methanolic and ethanolic extracts, both extracts showed similar antibacterial and antioxidant activity [13].
E. angustifolium extracts were active against both Gram-positive and Gram-negative bacteria.Lower activity against Gram-negative than Gram-positive bacteria was found [56,63,64,68,70,71,73].This susceptibility of Gram-positive bacteria has often been observed in antimicrobial studies on plant extracts, e.g., sage [87], Artemisia vulgaris [88], Eucalyptus grandis [89], etc., and it is attributed to the structure of the cellular membrane.Gram-negative bacteria have an outer membrane that restricts diffusion of compounds through its lipopolysaccharide wall and makes them more resistant to natural compounds or antibiotics [90,91].
Among Gram-positive bacteria, a high antibacterial effect expressed as a low MIC value (<300 µg/mL) or as the inhibition zone (>20 mm) was recorded for S. aureus [55,67,69,72] and B. cereus [67,68].It is worth noting that tested extracts were effective against clinically isolated strains of S. aureus [63] including MRSA strains [55] and M. luteus [63].Methicillinresistant Staphylococcus aureus (MRSA) strains cause serious and mortal infections in the elderly and in immunocompromised patients [92,93].Therefore, MRSA strains are considered as a very urgent health problem and in 2017, the WHO posted MRSA on a list of global priority pathogens classified as high priority (priority 2) [94].According to the literature, the leaf extracts of Polyalthia longifolia [95], Xylopia pancheri [96], Cistus salviifolius, and Punica granatum [91], and other species demonstrated antibacterial properties against clinical isolate strains of S. aureus including MRSA.Interestingly, punicalagin (ellagitannin) and ellagitannin-rich extracts from C. salviifolius and P. granatum were the most active against 100 S. aureus (50 MRSA) clinical isolates [97].In another study, ellagitannins such as salicarinin A, rugosin D, casuarictin, tellimagrandins I and II, pentagalloylglucose, stachyurin, casuarinin, vescalagin, castalagin, rugosin E, sanguiin H-6, and lambertianin C significantly inhibited growth of methicillin-susceptible strains of S. aureus [98].Regarding diversity and high concentration of ellagitannins in E. angustifolium ethanolic and aqueous extracts, this group of secondary metabolites might be responsible for the anti-staphylococci effect.However, detailed phytochemical studies are needed to make definitive conclusions.
Another Gram-positive bacterial species, Micrococcus luteus, was sensitive to fireweed extracts [63].E. angustifolium extract inhibited the growth of M. luteus more effectively than vancomycin or tetracycline (250 µg/mL).Unfortunately, the extract was not chemically studied, and no pure compounds were comparatively evaluated.Therefore, it is difficult to claim what active compound or class of secondary metabolite was responsible for this antibacterial effect in this case.It is worth noting that the digested extracts tested on probiotic bacteria (Lactobacillus and Bifidobacterium strains) did not inhibit the growth of these beneficial intestinal bacteria [74].This is important in the context of fireweed plants' utilization in herbal dietary supplements or teas.
Among Gram-negative bacteria, the best results (<300 µg/mL; >20 mm) were recorded for E. coli [55,67,69,72,74,75], K. pneumoniae [55,59,66], P. aeruginosa [55,59,66,67] A. baumannii [55], and S. Typhi [67].E. coli strains showed varying sensitivity to E. angustifolium extracts.Generally, the fireweed extracts showed moderate activity against E. coli.The best effect was observed for the crude whole plant extract [63], which inhibited the growth of bacteria in culture more effectively than vancomycin and tetracycline (250 µg/mL).However, the authors pointed out that the limitation of their study was lack of a dose response determined for select dilutions of the crude plant extract and lack of phytochemical analysis of the extract.
A methanolic extract of E. angustifolium also showed significant activity against P. aeruginosa and K. pneumoniae with a 23-25 mm inhibition zone mainly due to the properties of the solvent [66].Aqueous and ethanolic extracts were less effective against the same strains tested in this study.
A. baumannii and P. aeruginosa are pathogens with an urgent need for new antimicrobials, and their carbapenem-resistant strains are included in the WHO list of global priority pathogens classified as critical priority [94].High activity against these clinically isolated members of ESKAPE drug-resistant pathogens was noted using bio-hybridized nanocellulose films which consist of silver nanoparticles synthesized with aqueous extract of E. angustifolium [55].Among the test pathogens, methicillin-resistant S. aureus, K. pneumoniae, and E. coli strains were most sensitive (MIC = 0.625 µg/mL).The lowest activity was observed against A. baumannii strains (MIC = 1.25 µg/mL).The literature provides evidence of the effectiveness of biocidal nanomaterials against resistant bacteria [99,100].Nano silver hybridized onto bacterial cellulose films showed significant activity against E. coli and S. aureus [101], or S. aureus and P. aeruginosa [102].Green synthesized nanoparticles from Plantago major [103], Prunus africana, Camelia sinensis [104], and Tradescantia pallida extracts showed significant antimicrobial effects against S. aureus, E. coli, and P. aeruginosa [105].In another study, the MIC values of green synthesized silver nanoparticles against S. typhimurium and Y. enterocolitica were 6.2 µg/mL and 3.1 µg/mL, respectively [106].It is worth noting that the MIC/MBC values were significantly lower than those of antibiotics against tested pathogens.In this context, the low MIC values against MDR pathogens obtained by Baker et al. [55] seemed to be very promising and have potential for application in the biomedical sector.The safety issue of nanoparticles is still under discussion, but green synthesis has been regarded as safer, more environmentally friendly, non-toxic, and effectively scaled up for large-scale synthesis [107].

Antifungal Activity
E. angustifolium extracts showed strong antifungal properties against the dermatophytes M. canis [59] and T. tonsurans [67] with the MIC values of 10 µg/mL and 7.8-15.6µg/mL, respectively.It should be noted that the MIC value of 3 µg/mL is considered as a concentration limit for the effectiveness of drug therapy in M. canis or T. rubrum in humans [108].Significant antimycotic activity was also recorded against T. rubrum, T. mentagrophytes, and Arthroderma spp., with A. crocatum being the most sensitive species [67].In contrast to dermatophytes, the E. angustifolium extracts were inactive or moderately active against Candida spp. with the exception of C. tropicalis [67] and C. maltose [75].
The resistance of fungal species, i.e., Aspergillus and Candida spp., is well documented [109], whereas in other dermatophyte species, it is rather seldom reported, but is verified in T. rubrum, T. mentagrophytes [110,111], and M. canis [112][113][114].Therefore, plants that possess antidermatophytic properties might be potent as selective inhibitors of fungal activity and have economic value.It is worth noting that in another study on the related species Epilobium parviflorum, the average MIC values against the eight tested strains of T. mentagrophytes isolated from patients was 9.25 mg/mL [115].In this regard, E. angustifolium extracts demonstrated much stronger activity, expressed in the MIC value range of 62.3-125 µg/mL [67].
Cymbopogon spp., Eucalyptus robusta, Punica granatum, and S. baicalensis are plant species with strong antifungal properties with an MIC value range of 12.5-100 µg/mL [116].The main active compounds with proven antifungal activities are thymol, carvacrol, resveratrol, wogonin and other flavonoids (catechin), alkaloids (e.g., berberine, magnoflorine), gallic acid and other polyphenols such as tannins, terpenoids, and saponins.Gallic and ellagic acids as well as tannins have shown antifungal activity in numerous studies [85,117,118].These compounds are natural constituents of E. angustifolium extracts and contribute to the antifungal and antibacterial activities.However, the exact antifungal components of E. angustifolium extracts and their mechanism of action are still unknown.

Active Compounds of E. angustifolium Extracts and Mechanisms of Action
Most authors have attributed the antimicrobial activity of fireweed extracts to their rich polyphenol composition [13,56,64,67,69,71,76].The antimicrobial properties of polyphenolic compounds are linked to their absorption by the bacterial membranes, which leads to membrane perforation and leakage of cellular contents [85].The effect depends on the composition and concentration of phenolic compounds and their interactions.Polyphenols are also the main factors responsible for apoptotic and cytostatic activity of E. angustifolium extracts [12,14,119] as well as DNA binding properties [13].Recently, Peru ży ńska et al. 2023 [14] tested the ethanolic extract of E. angustifolium on different lines of cancer cells using bacterial cellulose membranes as the matrix for the controlled delivery of the plant extract.The SEM analysis confirmed the deposition of active compounds inside the membranes and the cytotoxicity effect of the extract was dose and time-dependent.In other words, they confirmed that E. angustifolium extract was able to efficiently penetrate the bacterial membranes and induce apoptosis in the cancer cells.
Neumann et al. 2022 [55] tested 14 plant extracts and found a significant correlation between the antimicrobial activity and tannin yield in the extract, but not flavonoids.The E. angustifolium leaf extract, rich in tannins (10.41%) and flavonoids (1.9%), was the most active against yeast (e.g., Candida maltose) and S. aureus.In this study, the antibiofilm effect of plant extracts against MDR E. coli strains was also investigated.Polygonum bistorta extract was most active (MIC 10 µg/mL), whereas E. angustifolium extract showed moderate activity (50-100 µg/mL).The result of correlation analysis of anti-biofilm activity and tannin content was moderate but statistically significant, but no correlation between flavonoids and anti-biofilm activity was found.This was surprising, because flavonoids are biofilm-inhibiting compounds.The authors explained that the assay used to evaluate biofilm inhibition did not reflect the full potential of the flavonoids and in the applied method, flavonoid content was calculated as hyperoside, which has comparatively low anti-biofilm activity.Regardless of this study, tannins and flavonoids are considered as the main contributors of antimicrobial activity [120][121][122].
Tannins might inhibit bacterial growth using different mechanisms such as inhibition of cell wall synthesis, iron chelation, disruption of the cell membrane, or inhibition of the fatty acid biosynthetic pathway [123].The biological function of ellagitannins in plants is defensive, with an anti-herbivore role [124,125].Their bioactivity can be attributed to the increasing number of hydroxyl groups present in the chemical structure, and in particular to the pyrogallol-type (1,2,3-trihydroxyphenyl) motif of the galloyl and galloyl-derived groups as well as to the substitution pattern and size of the ellagitannins [39,124].The size of molecules and oligomeric linkage of ellagitannins might influence the antimicrobial activity.Dimers with an m-DOG linkage exhibited stronger inhibition than ellagitannin oligomers with m-GOD or m-GOG linkages [98].
The antibacterial effect of ellagitannins against Staphylococcus aureus, Escherichia coli, and Clostridium perfringens was documented [98].The effect was the greatest against S. aureus and the weakest for C. perfringens.Salicarinin A and rugosin D inhibited the growth of S. aureus completely at a 0.5 mM concentration.Tellimagrandin II, rugosin D, agrimoniin, sanguiin H-6, and lambertianin C were the most efficient compounds against E. coli.The antibacterial effect against S. aureus was not clearly related to the molecular size or flexibility of ellagitannins; however, rugosins E and D and pentagalloylglucose with four or five free galloyl groups demonstrated stronger activity than the other ellagitannins with glucopyranose cores.The oligomeric linkage of ellagitannin and the number of free galloyl groups in pentagalloylglucoses were suggested as crucial factors in activity against E. coli.In turn, the antimicrobial effect against C. perfringens was attributed to the molecular size of ellagitannins.It should be added that macrocyclic ellagitannins such as oenothein B or A were not tested in this study.Oenothein B demonstrated antimicrobial properties against Helicobacter pylori, Staphylococcus aureus as well as fluconazole-sensitive and -resistant Candida albicans, C. tropicalis, and C. glabrata strains [126][127][128].Molecular docking analysis proved that oenothein B interacted with specific amino acid residues located at the active site of S. aureus lactoferrin, whereas the phenolic hydroxyl groups were not associated with lactoferrin [128].
The antimicrobial activity of fireweed extracts was also related to the presence of flavonoids, particularly to quercetin and kaempferol [64,71,74].Some authors attributed the antibacterial effect to the presence and the concentration of hydroxycinnamic acids [68,70,71].
Quercetin and its derivatives, which are the predominant compounds among E. angustifolium flavonoids, are known to have antibacterial and antiviral properties [129,130].In turn, myricetin-3-O-rhamnoside (myricitrin) was effective against B. cereus, E. coli, S. aureus, and K. pneumoniae [131,132].Flavonoids act via various mechanisms including suppression of nucleic acid synthesis, cytoplasmic membrane and energy metabolism, disturbances in adhesion and biofilm formation, inhibition of cell envelope synthesis, or membrane disruption [122,133,134].The antibacterial effect of flavonoids is linked to their chemical structure, particularly to the presence, number, and position of functional groups: hydroxyl, methoxy, halogen, and methyl in two aromatic rings A and B [134].
Significant antimycotic properties of gallic, ellagic, and chlorogenic acids have been mentioned above.However, antibacterial activity against methicillin-resistant S. aureus [135], MDR E. coli strains [136], and H. pylori [137] has also been reported.Recently, ellagic and gallic acids showed antibacterial potential against clinical IBD (inflammatory bowel diseases) isolates [117].4-O-caffeolyqunic, neochlorogenic, and chlorogenic acids were identified as the most potent anti-inflammatory constituents of E. angustifolium extract [24].Chlorogenic acid is an effective antimicrobial against both Gram-positive and Gramnegative bacteria [138][139][140].The antimicrobial mechanism of activity of chlorogenic acid is explained by disruption of intracellular and outer membranes and consequently cell metabolism [141,142].A more recent study indicates that chlorogenic acid may induce its action via downregulating ribosomal subunits, affecting lipid metabolism, and scavenging intracellular ROS [139].
The antibacterial activity of extracts might also be modulated and affected by the presence of other compounds.Notably, C. salviifolius extract was more effective against MRSA isolates, whereas P. granatum extract was more active against methicillin-sensitive S. aureus (MSSA) isolates [97].Both extracts were abundant in hydrolysable tannins but C. salviifolius extract contained more flavonoids (myricetin and quercetin derivates), phenolic acids and coumarins.E. angustifolium plants are a rich source of hydrolysable tannins and flavonoids.Considering the chemical composition of E. angustifolium extracts and the antimicrobial potential of their constituents, the synergistic mechanism of action should be suggested rather than the activity of a certain compound at high concentration.

Materials and Methods
A systematic literature search of the electronic databases of Scopus, PubMed/Medline, and Google Scholar was conducted for peer reviewed articles to find studies focusing on antibacterial and antifungal properties of Epilobium angustifolium L. extracts.The time range was set between January 2000 and June 2023.In all searches, the following terms were used: "Epilobium angustifolium"/"Chamerion angustifolium"/"Chamaenerion angustifolium" and "antibacterial"/"antifungal"/"medicinal plants with antibacterial properties".The search was not limited to title, keywords, and abstract, but the text of the articles was also explored.The obtained records of databases were further explored for articles that may match the search criteria.Studies were considered if they met the following criteria: (1) plant species-Epilobium angustifolium, (2) extracts prepared from plant material (roots, leaves, aerial parts, flowering aerial parts, seeds), (3) antibacterial or antifungal studies, (4) article written in English.Essential oils, honey, food supplements, and cosmetic products were excluded.The literature search yielded the following number of records: Scopus (29), PubMed/Medline (79), and Google Scholar (123).Reviews, conference proceedings, and articles concerning biological activities of E. angustifolium extracts without antimicrobial activities were not included.After rigorous selection, removing duplicates and irrelevant articles, only 23 articles were considered for this review (Table 1).

Conclusions
The reviewed literature showed a broad spectrum of antimicrobial activity of E. angustifolium extracts and provided scientific evidence for the traditional utilization of this species.The studies demonstrated significant variation of antimicrobial activity depending on the tested species and strains, type of extract solvent, or plant organs utilized for the extract preparation.E. angustifolium extracts demonstrated antibacterial activity against both Gram-positive and Gram-negative bacteria and showed antimycotic effects against the fungi of M. canis and T. tonsurans and the dermatophytes Arthroderma spp.Greater susceptibility of Gram-positive than Gram-negative bacteria to fireweed extracts was found.A strong antibacterial effect expressed in the low MIC values (<300 µg/mL) or as in inhibition zones (>20 mm) was recorded for S. aureus, B. cereus, E. coli, K. pneumoniae, P. aeruginosa, E. coli, S. Typhi, and A. baumannii including methicillin-resistant strains.The antimicrobial activity of fireweed is attributed more to the rich polyphenol composition of extracts and their synergic interactions than the activity of a certain compound at a high concentration.Although the standardization of antimicrobial studies and the methods of chemical analysis of extracts have improved over the last decade, there is still a need for further improvement of methodological quality and comparative studies including isolated substances.Another challenge is the lack of adequate knowledge on the compositions and mechanisms of action of extracts.Nevertheless, the interesting results of the cited studies suggest potential utilization of fireweed extracts as antimicrobials in wound healing, components of cosmetic products for humans and animals, or a food preservative.

Table 1 .
Studies testing antimicrobial activity of Epilobium angustifolium extracts.