Biosensors and Bio-Bar Code Assays Based on Biofunctionalized Magnetic Microbeads

This review paper reports the applications of magnetic microbeads in biosensors and bio-bar code assays. Affinity biosensors are presented through different types of transducing systems: electrochemical, piezo electric or magnetic ones, applied to immunodetection and genodetection. Enzymatic biosensors are based on biofunctionalization through magnetic microbeads of a transducer, more often amperometric, potentiometric or conductimetric. The bio-bar code assays relie on a sandwich structure based on specific biological interaction of a magnetic microbead and a nanoparticle with a defined biological molecule. The magnetic particle allows the separation of the reacted target molecules from unreacted ones. The nanoparticles aim at the amplification and the detection of the target molecule. The bio-bar code assays allow the detection at very low concentration of biological molecules, similar to PCR sensitivity.


Introduction
Magnetic microbeads (MMB) used in biomedical applications present usually a core/shell structure. Such microbeads have an inorganic core e.g. iron oxide, surrounded by an outer layer of shell wall that consists of long-chain organic ligands or inorganic/organic polymers. The attachment of bioactive ligands to the surface of the outer shell is the key to bioapplication of magnetic microbeads. Table 1 summarizes the main immobilization procedures of bioactive species, on magnetic microbeads used in this chapter. A more exhaustive view can be found in [1]. This paper is devoted to biosensing applications of magnetic microbeads. It is divided into three parts covering three biosensing systems: affinity biosensors, enzymatic biosensors, bio-bar codes.  [2], [3] Amine Maleimido groups: SMPB sulfo-SMCC Thiol derivatives [4], [5] Gold Thiol Thio-DNA Simple Limited to thiol containing molecules [6] Active ester Amine Avidin, Streptavidin Biotinylated biomolecules Antibody Simple Need biotinylated biomolecules [7] 2. Use of magnetic microbeads for affinity biosensors

Immunomagnetic electrochemical sensors (ELIME)
Electrochemical immunosensors are designed through the immobilization of the specific antibody on the surface of the electrochemical transducer. The main problem affecting immunosensors is reproducible regeneration of the sensing surface. The need of renewal of the sensing surface arises from the affinity constants derived from the strong antigen-antibody interaction. This renewal is a difficult task since the drastic procedures required alter immunoreagent bound to the surface of the transducer. This drawback makes immunosensors difficult to be integrated into automatic systems. An alternative approach avoiding regeneration consists of using disposable antibody-coated magnetic microbeads and building up in situ immunosensing surface by localizing the immunomagnetic beads on the electrode area with the aid of a magnet. Moreover, the use of immunomagnetic beads is particularly evident in the detection of analytes contained in complex sample matrices (e.g. heterogeneous food mixtures) that may exhibit either poor mass transport to immunosensor or physical blockage of immunosensor surface by non-specific adsorption. The schematic representation of the enzyme-linked immunomagnetic electrochemical assay (ELIME) is presented in figure 1. Immunogenic analyte (bacteria, for example) is sandwiched between an antibody-coated magnetic microbead (immunomagnetic bead) and an antibody-enzyme conjugate. The immunomagnetic bead is trapped magnetically on the electrode surface, exposed to the enzymatic substrate, the electroactive product is detected electrochemically. This type of immunomagnetic electrochemical assay was applied for different analytes with different transducer/enzyme combinaisons, gathered in table 2. A good reproducibility of 2% relative standard deviation was observed [8]. (ELIME) (Reprinted from [9] with permission from Elsevier).

Label-free immunomagnetic impedancemetric sensors [7]
The electrochemical impedance measurements of the electrical properties of an antibody layer immobilized on a gold electrode allows the direct monitoring of the variation of these properties when antigen-antibody interaction occurs. This technique allows label-free detection of the antigen concentration in biological samples. The problem of regeneration of the sensing surface has been solved, in this example, by using streptavidin magnetic microbeads for the immobilization of the antibody specific of a small pesticide molecule, the atrazine. The antibody, biotinyl-anti-atrazine Fab fragment K47, forms a quite stable layer onto the streptavidin-magnetic microbeads immobilized on gold electrode using a 300mT magnet, due to the high affinity of the biotin/ streptavidin interaction. After the antibody layer formation an antigen, atrazine was injected and interacted with the antibody (cf figure 2). Complex impedance plots of the successive building-up of the sensing layers: magnetic microbeads/ Au electrode, biotinyl-Fab fragment K47 antibody/ magnetic microbeads/ Au electrode and after a 600 ng/ml of atrazine injection in cell are shown in figure 3.
Using Randles' equivalent circuit model, an excellent fitting between the simulated and experimental spectra was obtained. The electron transfer resistance values were 304.6 Ω.cm 2 for the magnetic monolayer, 204.5 Ω.cm 2 for the antibody layer and 188.5 Ω.cm 2 after injection of 600 ng/ml of atrazine. The decreases of electron transfer resistance could be attributed to a reorganization of the microbead layer as the specific capacitance decreases too. The specific capacitance, extracted from the computer fitting for the same steps was 17µF/cm 2 , 15µF/cm 2 and 14.29µF/cm 2 , respectively. This decrease should be due to a thickness increase.
In order to obtain a calibration data set, the values of electron transfer resistance differences ∆R et versus the added atrazine concentrations were plotted, as shown in figure 4. The change of electron transfer resistance was calculated according to the equation: Where R et(Ab) is the value of electron transfer resistance after antibody immobilization, R et(Ab-Ag) is the value of the electron transfer resistance after antigen binding to the antibody. As it can be seen in figure 4, the plot is linear for high concentrations of atrazine and then reaches saturation. A linear relationship between ∆R et values and the concentration of atrazine was established in the range from 50 to 500ng.ml -1 . A detection limit of 10ng.ml -1 is reached.

Piezoimmunosensors
A piezoelectric immunoassay based on a quartz crystal microbalance (QCM), with a renewable surface, was also proposed using immunomagnetic beads localized on the surface of a quartz using a permanent magnet [13]. Human IgG as analyte was detected using goat-anti-human IgG antibody covalently bonded on aminomodified silica coated magnetic microbeads. A detection limit of 0.36µg.ml -1 and a dynamic range of 0.6-34.9µg.ml -1 were obtained using a 9MHz QCM.
Another piezoelectric immunosensor, based on a 5MHz quartz crystal resonator, was used for the detection of biological pathogens such as Salmonella typhinium [14,15]. The Salmonella cells were captured by antibody-coated magnetic microbeads and then these complexes were moved magnetically to the sensing quartz and were captured by antibodies immobilized on the crystal surface. An impedance analyser measured the impedance behavior of the oscillating quartz crystal exposed to various concentrations of Salmonella typhinium (10 2 -10 8 cell per ml). The response of the crystal was expressed in terms of equivalent circuit parameters. The motional inductance and the motional resistance increased as a function of the concentration of Salmonella. The viscous damping was the main contributor to the resistance and the inductance in a liquid environment. The load resistance was the most effective and sensitive circuit parameter. A magnetic force was a useful method to collect complexes of Salmonella-immunomagnetic microbeads on the crystal surface and enhance the response of the sensor. In this system, the detection limit, based on resistance monitoring was about 10 3 cell per ml.

Genomagnetic electrochemical assays
Currently developed DNA hybridization sensors are using single-stranded (ss) short (15-25 nucleotides) oligodeoxynucleotides (ODN, probe DNA) immobilized on an electrode. The ODNmodified electrode is immersed in target DNA solution. When the sequence of target DNA matches that of the probe (based complementary Watson-Crick pairing), a probe-target (hybrid) duplex DNA is formed at the electrode surface. The hybridization event (DNA duplex formation) is detected electrochemically in different ways. This system works quite well with synthetic ODNs when probe target DNAs are about the same lengths. In a real DNA sequence analysis with longer PCR products, viral or chromosomal DNAs, the target DNAs are substantially longer than the probe. With longer target DNAs, difficulties connected with non-specific target DNA adsorptions frequently arise, resulting in a loss of specificity and decreased sensitivity. Elimination of the non-specific DNA adsorption at the electrodes (such as carbon or gold ones) usually applied for the DNA hybridization is very difficult. A new method based on separation of DNA hybridizal step (on magnetic microbeads) from the electrochemical detection step has been proposed and successfully used [16,17]. One procedure of the genomagnetic electrochemical bioassay (GEME), based on a PNA probe and on an electroactive intercalator, is schematized in Figure 5 [18]. The detection of target DNA can be done by label-free detection of guanine peak [16,17,19], by detection of chemically modified DNA prelabelled or postlabelled by redox probes [16], by detection of the product of the enzymatic reaction on DNA post-labelled by enzyme [20], or by detection of redox intercalators [18]. The features of the different genomagnetic electrochemical assays are presented in Table 3. Magnetic microbeads are now used as magnetic markers for bioassays. Measurements based on variation of magnetic permeability were performed using a transducer comprising a coil placed in a Maxwell bridge [21] (see Figure 6). The change in magnetic permeability of a compound is detected using inductance measurements. Furthermore, analyte detection has been achieved directly (the analyte being labelled ferromagnetically) or competitively (competition between ferromagnetic labelled and unlabelled analytes exists for binding sites on the transducer) using the aforementionned device. The use of magnetic markers has been shown to offer advantages such as, low interference, little or no background signal, no transducer fouling and no sample treatment. The 'sandwich' approach used in the magneto binding assay, where the target analyte is bound between silica carrier particles and the magnetic marker is presented in Figure 7.  The 'sandwich' approach used for in the magneto binding assay (Reprinted from [21] with permission from Elsevier).
Histone H1 conjugated magnetic microbeads were assessed for their ability to work as magnetic transducers for the detection and quantification of DNA [22]. For the quantification of calf thymus DNA, a linear relationship between the DNA concentration in the sample and the relative magnetic permeability of the pellet was found for DNA concentration up to 67µg.ml -1 in buffered solutions as well as in a lyzed cell culture. The detection limits were determined to be 12 and 31µg.ml -1 , respectively. For the quantification of plasmid DNA in buffered solution a linear range was established for concentration up to 150µg.ml -1 and the detection limit was determined to be 52 µg.ml -1 .
A rapid (6.5min) and simple one-step magnetic immunoassay has been developed for the analysis of human urinary albumin in near patient settings [23]. Polyclonal rabbit anti-human was used as a capture antibody and monoclonal mouse anti-human albumin as a detection antibody in a two-site immunomagnetic assay, requiring no additional washing procedures. The polyclonal anti-human albumin was conjugated to silica microparticles (cf Figure 7) and the monoclonal antibody to dextran coated magnetic microbeads (cf. Figure 7). Quantification of human albumin in undiluted urine was performed by adding 2µl urine to a measuring vial containing polyclonal Ab-silica microparticles, monoclonal Ab-magnetic microbeads and reaction buffer and then agitating the vial by hand for 20s. A linear response was obtained for 0-400µg.ml -1 albumin with a detection limit of 5µg.ml -1 .

Frequency mixing
A new technique so-called frequency mixing was also used for detection of magnetic biomarkers. A two-frequency magnetic field excitation was used and the magnetic response at a third frequency was detected, which is a linear combination of the applied frequencies [24]. The so-called magnetic sandwich bioassay for the detection of c-reactive protein (CRP) is presented in figure 8. The detection principle is based on two different anti-CRP antibodies (monoclonal IgG) for CRP trapment (grafted on a polyethylene PE sintered filter) and labelling. The linear detection range of this immunosensor ranged from 25 ng.ml -1 to 2.5 µg.ml -1 and is therefore much more sensitive than typical hsCRP-sandwich-assays. Disavantages of this system is the sample size. Sample sizes are currently 0.5 ml, which is quite high for blood serum analysis.  [24] with permission from Elsevier).

Magnetic AFM [25]
For developing a magnetic bioassay system, an investigation to determine the presence of a specific biomolecular interaction between biotin and streptavidin was done using paramagnetic nanoparticles and a silicon substrate with a self-assembled substrate (cf figure 9). The reaction of streptavidinmodified magnetic nanoparticles on the biotin-modified substrate was clearly observed under an optical microscope (cf. Figure 10). The magnetic signals from the particles were detected using a magnetic force microscope (MFM). The results of this study demonstrate that the combination of a monolayer-modified substrate with biomolecule-modified magnetic particles is useful for the detection of biomolecular interactions in medical and diagnostic analysis.

Magnetic Resonance Imaging (MRI)
An application of magnetic nanobeads is the development of in situ sensors. Based on biomolecular interaction, the self assembly of the superparamagnetic iron oxide core nanobeads can be realised, leading to the enhancement of the spin-spin relaxation times of the water molecular surrounding the self-assembly [26]. Indeed, the spins of surrounding water protons are more efficiently dephased. Therefore if one can design magnetic nanobeads that would self-assemble in the presence of a target and detect the T2 relation time of water protons, it would be possible to design a magnetic nanosensor for magnetic resonance imaging. A proof of concept was realised using DNA/DNA interaction, protein/protein interactions, protein/small molecules and enzyme reactions [26].
After immobilising serotonin on dextran caged iron oxide nanobeads (75nm), Perez et al [27] have shown that in the presence of Horse radish peroxydase or Myeloperoxidase and hydrogen peroxide, self assembly of the nanoparticles was observed leading to a shift in T2 relaxation that could be successfully imaged. (cf figures 11 and 12) Figure 11. Principle of peroxidase based assay. In the presence of peroxidase, free radicals are formed allowing self assembly (Reprinted from [27] with permission from ACS). This group performed similar experiments for the detection of andeno virus and herpes virus (5 viral particles/10 µl) by immobilising antibody directed against these viruses at the surface of dextran caged iron nanoparticles [28] (cf figure 13). The antibodies were immobilised via the protein G attached to the dextran via N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP).
Harris et al [29] have developed an MRI assay with nanoparticles (50 nm dextran caged iron core nanoparticles) for detecting Matrix Metalloproteinase-2 (MPP-2) activity. This protease activity when upregulated leads to invasive proliferation and metastasis of cancer cell. The strategy proposed by these authors relies on a peptide modified PEG that blocks the interaction between neutravidin and biotin. The peptide has an amino-acid sequence which is specific to MPP-2 protease activity. In the presence of MPP-2, PEG is removed from the particles by enzymatic cleavage. The particles can then assemble into nanoassemblies due to the interaction of biotin and neutravidin leading to a T2 shift (cf figure 14). The device could detect down to 9.2 Uml -1 of MPP-2. The authors have demonstrated that inhibition of biotin/neutravidin interaction increases with PEG molecular weight (10 kDa). Inversely, the rate of aggregation in the presence of MPP-2 increases with lower PEG molecular weight. In the presence of a MMP-2 inhibitor (Galardin), no aggregation occurs and then no variation in the T2 relaxation time is observed (cf figure 15).
In conclusion, self assembling of magnetic nanoparticles upon biological interaction is a promising tool for in vivo diagnostic as demonstrated. Indeed these authors have shown that this methodology can work in complex and turbid media. Its sensitivity is below the concentration encountered in tumoral cell for instance.   In the presence pf an MMP-2 inhibitor (Galardin) no variation in the T2 relaxation time is observed. 5c) aggregation is observe when incubating the nanoparticles with HT-080, while it is inhibited in the presence of galardin (Reprinted from [29] with permission from Wiley VCH).

Enzymatic biosensors based onto magnetic microbeads
Most of proposed enzymatic biosensors are based on biofunctionnalization of a transducer more often amperometric, potentiometric or conductimetric. Compared to classical immobilization methods used for the development of enzymatic biosensors, the use of magnetic microbeads can bring a lot of advantages: 1. enzyme immobilization onto magnetic microbeads, having high specific area, allows a high loading of the sensitive matrix 2. such a matrix provides a good macroenvironment for retention of the bioactivity 3. the most decisive advantage is a good control of the localisation of the sensitive material through the use of magnets or of magnetised transducing parts allowing enzyme reactions to occur close to the detection device 4. the sensitive transducing part can be easily renewed In parallel with biosensing applications, enzyme coated magnetic microbeads have been used to facilitate the enzyme handling. By this way, stock solutions containing such a material can be, when properly stored, used during for more than ten months without significant activity loss. Then this immobilization method has been for example validated for glucose oxidase, urease and alpha-amylase covalently immobilized onto polyacrolein beads [30].
Except the work of Miyabayashi et al. [31], where biomodified magnetic beads were used on a Clark electrode for glucose or Saccharomyces cerevisiae detection, one of the first applications of magnetic beads for biosensors was the combination of a covalent enzyme bonding onto magnetic beads with physical entrapment on the sensor surface. Latex beads, containing superparamagnetic material covalently modified with enzymes, have been patterned on a transducer by the means of screen printed thick films permanent magnets. Such a method, valuable for batch production independently of the nature of the substrate material, was evaluated for assessing glucose concentrations up to 20mM [32].
Enzymes immobilized on magnetic microparticles can be trapped by magnets and retained on an electrode surface at a specific location in flow analysis devices. But only few works using magnetic microparticles have been devoted to design real enzymatic biosensors. As an example of such devices, an enzyme-based electrochemical magnetobiosensor for environmental toxicity analysis can be quoted. The integration of the bioactive material as urease, cholinesterases classes, significantly increases the sensitivity and allows detection limits as low as 10 -11 M for heavy metal ions and 10 -12 M for organophosphates and carbamates [33]. In table 4, characteristics of some magnetic microbeads based enzymatic biosensors are gathered. As an example a strategy of enzyme immobilization is given in figure 16 where a covalent bonding of the biospecie can be achieved allowing a quite good storage stability. Figure 16. Preparation of magnetic bio-particles for a tyrosinase based biosensor (Reprinted from [3] with permission from Elsevier).
Recently, the potentialities of nanoporous silica microparticles containing superparamagnetic defect spinel-type iron oxide nanoparticles inside their pores have been underlined for biosensors applications. This approach allows the obtaining of a high sensitivity and selectivity even in complex media. Interest of such a support has been validated for the immobilization of horseradish peroxidase leading to hydroquinone and hydrogen peroxide biosensing at level as low as 4x10 -7 M [36]. The design of a magnetised carbon paste electrode with trapped magnetic nanoporous silica microparticles (MMPs) is given on figure 17. A similar strategy has been used for the immobilization of horseradish peroxidase with a high density of nanopores of MMPs having a diameter of 5 micrometers and applied for studying peroxidation of clozapine. Clozapine is a drug belonging to the dibenzoazepine class and often used to treat neurological disorders. The resulting amperometric biosensor allows the drug quantification in the micromolar range and presents a quite good stability, no significant signal loss being observed after one month of storage [37].
Enzymatic MMPs based devices present also specific advantages, as MMPs do not act as a barrier for the diffusion of the analyte to the electrode surface. Only a decrease of few percent of the voltamperometric signal is observed in presence of microparticles compared to the bare electrode. Furthermore MMPs constitute a valuable tool for inhibition studies, immobilized enzymes on such macroparticles being less sensitive, through a screening effect, to inhibitors than soluble enzymes. Thus, it has been recently shown that immobilized HRP, through MMP strategy, was protected from inactivation by inhibitors as thiols which can react with intermediary quinoneimine derivatives produced during the enzymatic reaction [38].
Magneto-switchable electrocatalysis and bioelectrocatalysis are accomplished by the surface modification of magnetic microbeads with redox-relay units. By the attraction of the modified magnetic microbeads to the electrode support, or their retraction from the electrode, by means of an external magnet, the electrochemical functions of the magnetic microbeads tethered relays can be switched between "ON" and "OFF" states, respectively. The magneto-switchable redox functionalities of the modified particles activate electrocatalytic transformations, such as a biocatalytic chemoluminescence cascade that leads to magneto-switchable light emission or the activation of bioelectrocatalytic processes [39,40].

Bio-bar code
Polymerase chain reaction was introduced in 1985 and has revolutionized biology and molecular biology since then. Its sensitivity allows the detection of 5-10 copies of DNA. However some drawbacks such as its complexity, time consuming procedure and narrow target have motivated the findings of new technologies.
Furthermore PCR is related to DNA detection, and some authors have been looking for new procedures allowing the detection of proteins with similar sensitivity offered by PCR.
The bio-bar code assay appeared in the early 2000's as a promising analytical tool for high sensitivity detection of protein [6] and DNA [4].
In the following section, the principle of bio-bar-code for protein and DNA detection will be presented followed by examples of biological applications.
The bio-bar code assay relies on a sandwich structure based on specific biological interaction of a magnetic particles and a nanoparticle with a defined biological molecule in a medium. The magnetic particle allows the separation of reacted target molecules from unreacted ones. The nanoparticles aim at amplifying and detecting the target of interest.
Therefore, the bio-bar code approach relies on two components: • Magnetic Micro Beads (MMB) bearing the biological probe (DNA or Antibody, Figure 18) for target recognition.
To be clear the oligonucleotide sequence immobilized on the Nanoparticles ( Figure 18) will be called bio-bar code complement, whereas the bio-bar-code ( Figure 18) will refer to the oligonucleotide that hybridised with this immobilised sequence. The bio-bar-code sequence is the one that is captured on the DNA chip and further detected. The steps involved in the bio-bar-code assay are presented in Figure 19. First, the MMB are added to a solution of interest and allowed to interact with the biological target to be detected generally via DNA/DNA interaction or Antibody/Antigen interaction (Figure 19.1). Next, the NPs are added to the mixture and interact with the biological target (via DNA/DNA interaction or Antibody/Antigen interaction) to form a sandwich like structure (Figure 19.2). This structure is separated from the medium and from unreacted material thanks to the magnetic properties of the MMB (Figure 19.3). The beads can then be separated from the medium (Figure 19.4). The sandwich is redispersed in pure water. The bio-bar code DNA is then dehybridized (Figure 19.5), purified from the particles by centrifugation (Figure 19.6). It is then captured on a DNA chip and detected by fluorescence or silver enhancement (Figure 19.7). A promising approach based on using a Y junction dendrimer like DNA carrying two fluorophores (Alexa Fluor 480 and Bodipy 630/650). The coding is not detected via a DNA chip but by intensity encoding. In consequence, no nanoparticles are required [41]. So far this approach was carried out on polystyrene based microbeads (non magnetic) but would be an interesting alternative in the bio-bar-code assay.

Nanoparticles (NP)
The NP is aimed at detecting and signal amplifying of the molecule through the so called bio-bar code, after the interaction with the MMB/target complex. Two materials are preferentially employed namely gold nanoparticles [2,4] and polystyrene [42].
Two different cases will be considered depending on the target molecule: 1) antigen detection and 2) nucleic acid detection. If the target molecule is an antigen, a polyclonal antibody (directed against the target molecule) is immobilised at the surface of the particles. In the case of polystyrene, amine bearing particles are used and the antibody is immobilised using a glutaraldehyde protocole through its Ntermini. Next, the bio-bar code complement is immobilised at the surface of the particles. In the case of gold NP, it is immobilised through thiol/gold interactions. In the case of polystyrene, immobilisation is achieved through amine glutaraldehyde strategy (1530 ± 181 Abs). The particles in the later case are passivated with ethanol amine.
The sequence is typically 30 to 50 bases long, it can bear at the 3' end an A10 spacer and an amine or thiol terminated alkyl (usually C6). Sequence analysis of the complement bio-bar code leads to the following: The GC content is near 50 % with a melting temperature between 65°C and 75°C (calculated with the next neighbouring theory).
The bio-bar code is loaded by hybridization at room temperature in PBS 0.1 M. The average number of loaded bio-bar code is estimated to be a few hundreds (approx. 360) [5] on 30 nm gold particles and 3.5 10 5 on polystyrene particles (1 µm diameter). Under these hybridization conditions on polystyrene particles, the hybridisation yield is near 10 % as observed by UV spectroscopy and fluorescence [42].
If the target molecule is a nucleic acid, two different oligonucleotides are immobilised at the surface of the particle using a solution containing both molecules. One is an oligonucleotide complementary to the target and one corresponding to the bio-bar-code complement. The ratio between the two oligonucleotides leads to signal amplification.
In 2005, Taxton et al developed simplified protocole of the bio-bar-code NP. Instead of using double stranded DNA, the bio-bar-code consists in thiol immobilized oligonucleotides on gold NP. The DNA is released for on chip DNA capture by DTT [43].

Magnetic Microbeads (MMB)
The Magnetic Microbeads aim at interacting with the target molecule through biological recognition, namely DNA/DNA complementary sequence (hybridization) or antigene/antibody interaction. It gives to the sandwich structure magnetic properties allowing its separation from unreacted material and medium.
Typically, Magnetic Micro Beads are iron oxide based particles that may be encapsulated in a polymeric layer, the total particle having a diameter in the range of one to several µm. Mirkin group used amine bearing MMB obtained by silanisation of iron oxide [5] with an amine modified silane or purchased with a polyamine layer [2,5]. Immobilisation of the monoclonal antibody is achieved by reaction of glutaraldehyde with the primary amine at the surface of the particle and with free amine of the antibody. In the case of oligonucleotide target complement, the particle is modified with surface maleimido groups using succinimidyl 4-(p-maleimidophenyl) butyrate (SMPB) [4] or sulfosuccinimidyl 4-N-maleimidomethyl cyclohexane-1-carboxylate (sulfo-SMCC) [5]. The oligonucleotide is then immobilised through thiol addition to the double bound of the maleimido group. The average number of DNA strand per particles is 3 10 5 . In the case of antibody immobilisation, the average number of immobilised molecules is 3500 (small) per MMB as estimated by optical density at 280 nm. Capping is accomplished with BSA or sulfo-NHS acetate.

Detection
Three main detection methods are described in the literature namely: scanometric detection, fluorescent detection and rolling circle amplification (RCA).
Scanometric detection is performed as followed. After collection of the sandwich structure from the media, the DNA bar code is released from the NP by dehybridization of the bio-bar-code from its complement at high strigency (in DI water 55 °C) or by competition between thiolated oligonucleotide and DTT [43,2]. The bio-bar-code is recovered from the supernatant after centrifugation and captured on DNA chip by hybridization. The DNA chip is usually supported on maleimide modified glass slides bearing 12 mer oligonucleotides. After hybridization of the bio-bar-code (30-50 mer) on the DNA chip, a labelled oligonucleotide gold nanoparticle (13 nm) further hybridizes with the free end of the bio-bar-code. The chip is then immersed in a modified photographic solution containing silver salt. Its reduction is being catalyzed by the gold nanoparticules. The bio-bar-code is then detected by light scattering at the surface of the chip due to the silver particles (around the gold nanoparticles).
Mirkin groups uses an alterative methods based on the detection of a fluorophore (Alexa type) instead of silver enhancement [42,44].
Scanometric detection leads to detection as low as 500zM [5] in the case of DNA targeted molecules and 3 aM is the case of protein targeted molecules (PSA) [42]. With fluorescence, the detection limit is of a few hundreds of aM. In the later case, the detection limit was lowered by using micrometer scaled NP instead of nanoscaled. Indeed 3.10 5 bio-bar-code were loaded per particles, as compared to a few hundreds. However one would thing that smaller size NP would allow a higher density of NPs per MMB compared to microscale NP.
In fact, when using complex media or when multiplexing, the detection limit is increased by one to five orders of magnitude probably due to cross reactivity and non specific adsorption [4].
Other authors have developed rolling circle amplification (RCA) coupled to the use of magnetic bead [45]. This method allows an antibody / antigen interaction to be revealed and amplified using a DNA polymerase reaction. The method, which can be used in several different formats (ELISA, array), has been described using magnetic particles as solid support. The magnetic beads (Figure20) have been functionnalized with polyclonal anti-human IgE antibodies (Figure 20  by incubating a DNA polymerase in the mixture (Figure 21.4). The extension goes on using the circular DNA as a template several times, then leading to the "rolling circle amplification": the final extended DNA includes several linear complementary copies of the initial circular DNA template (Figure 21.5). The analysis of this DNA is done using a labelled oligonucleotide probe that hybridizes on the extended DNA stand (Figure 21.6).
Such an assay has been compared to a more classical immunoassay using magnetic particle and an anti-IgE alkaline phosphatase conjugate. The RCA amplification gave approximately 75-fold more signal than the classical immunotest, for a quantity of 25 ng/ml of IgE to be detected. The limit of detection of the RCA assay was reported as 1 pg/ml.  Table 5 presents the summary of existing bio-bar code assays.

Biological applications
Most applications concern the diagnosis of cancer (PSA), pathogens (bacteria or viruses), the detection of Alzhiemer disease maker (ADDL).
In brief, Bio-bar-code allows the detection of biomolecules in the aM range when dealing with model solution. When detection is performed with a complex mixture for multiplex detection, the detection limit raises up to 500fM.
Furthermore Single Nucleotide Polymorphism selectivity was realized [43]. However, the assay was performed in with two separated solution with concentration of 500 aM. It did not allowed to test cross reactivity. The signal to noise ratio is not given. These limitations could be due to non-specific adsorption or cross-reactivity.

Conclusion
The Bio-bar code assays allow the detection at a very low concentration of biological molecules similar to PCR sensitivity. Further work will probably focus on optimising biological molecule immobilisation in order to reduce cross-reactivity and to lower non specific adsorption.