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Article

Impact of Experimental Parameters on Cell–Cell Force Spectroscopy Signature

1
Department of Materials Science and Engineering, Uppsala University, Ångströmlaboratoriet, Box 35, SE-751 03 Uppsala, Sweden
2
CNR-Nanoscience Institute-S3, Via Campi 213/A, 41125 Modena, Italy
3
James Watt School of Engineering, University of Glasgow, Glasgow G128LT, UK
4
Department of Physics, University of Genoa, via Dodecaneso 33, 16146 Genoa, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to the paper.
Academic Editor: Petr Skládal
Sensors 2021, 21(4), 1069; https://doi.org/10.3390/s21041069
Received: 28 December 2020 / Revised: 27 January 2021 / Accepted: 1 February 2021 / Published: 4 February 2021
(This article belongs to the Special Issue Cantilever-Based Sensors)
Atomic force microscopy is an extremely versatile technique, featuring atomic-scale imaging resolution, and also offering the possibility to probe interaction forces down to few pN. Recently, this technique has been specialized to study the interaction between single living cells, one on the substrate, and a second being adhered on the cantilever. Cell–cell force spectroscopy offers a unique tool to investigate in fine detail intra-cellular interactions, and it holds great promise to elucidate elusive phenomena in physiology and pathology. Here we present a systematic study of the effect of the main measurement parameters on cell–cell curves, showing the importance of controlling the experimental conditions. Moreover, a simple theoretical interpretation is proposed, based on the number of contacts formed between the two interacting cells. The results show that single cell–cell force spectroscopy experiments carry a wealth of information that can be exploited to understand the inner dynamics of the interaction of living cells at the molecular level. View Full-Text
Keywords: cell-cell interaction; force spectroscopy; atomic force microscopy; cell mechanics; mechanobiology cell-cell interaction; force spectroscopy; atomic force microscopy; cell mechanics; mechanobiology
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    Doi: 10.5525/gla.researchdata.1103
    Link: http://researchdata.gla.ac.uk/id/eprint/1103
    Description: This folder contains data associated to the paper "Impact of experimental parameters on cell-cell force spectroscopy signature" authored by Reinier Oropesa-Nuñez, Andrea Mescola, Massimo Vassalli, Claudio Canale
MDPI and ACS Style

Oropesa-Nuñez, R.; Mescola, A.; Vassalli, M.; Canale, C. Impact of Experimental Parameters on Cell–Cell Force Spectroscopy Signature. Sensors 2021, 21, 1069. https://doi.org/10.3390/s21041069

AMA Style

Oropesa-Nuñez R, Mescola A, Vassalli M, Canale C. Impact of Experimental Parameters on Cell–Cell Force Spectroscopy Signature. Sensors. 2021; 21(4):1069. https://doi.org/10.3390/s21041069

Chicago/Turabian Style

Oropesa-Nuñez, Reinier, Andrea Mescola, Massimo Vassalli, and Claudio Canale. 2021. "Impact of Experimental Parameters on Cell–Cell Force Spectroscopy Signature" Sensors 21, no. 4: 1069. https://doi.org/10.3390/s21041069

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