BactoSpin: Novel Technology for Rapid Bacteria Detection and Antibiotic Susceptibility Testing

Inappropriate use of antibiotics is one of the leading causes of the increasing numbers of resistant bacteria strains, resulting in 700,000 deaths worldwide each year. Reducing unnecessary use of antibiotics and choosing the most effective antibiotics instead of broad-spectrum drugs will slow the arms race between germs and humans. Urinary tract infections (UTIs) are among the most common bacterial infections. Currently, accurate diagnosis of UTI requires approximately 48 h from the time of urine sample collection until antibiotic susceptibility test (AST) results. This work presents a rapid bacterial detection device that integrates a centrifuge, microscope, and incubator. Two disposable microfluidic chips were developed. The first chip was designed for bacteria concentration, detection, and medium exchange. A second multi-channel chip was developed for AST. This chip contains superhydrophobic and hydrophilic coatings to ensure liquid separation between the channels without the need for valves. The designed chips supported the detection of E. coli at a concentration as low as 5 × 103 cells/mL within 5 min and AST in under 2 h. AST was also successfully performed with Klebsiella pneumonia isolated from a human urine sample. In addition, machine-learning-based image recognition was shown to reduce the required time for AST and to provide results within 1 h for E. coli cells. Thus, the BactoSpin device can serve as an efficient and rapid platform for UTI diagnostics and AST.


Introduction
According to the Centers for Disease Control and Prevention (CDC), more than 2.8 million antibiotic-resistant infections occur in the U.S. each year, and more than 35,000 people die as a result [1]. In addition to clinical burdens, approximately USD 3 trillion in annual GDP is lost due to excess health care and loss of productivity associated with antimicrobial resistance [2,3]. Inappropriate use of antibiotics is one of the leading causes of the increasing numbers of resistant bacteria strains [4]. A strong relationship was documented between country-wide antibiotic consumption and the prevalence of nonsusceptible bacteria [5]. Urinary tract infections (UTIs) are among the most common bacterial infections, affecting more than 150 million people worldwide annually [6]. Those who suffer from recurrent infections can have more than three cases of UTI per year [7]. Women are more prone to UTI, with more than 50% of women experiencing at least one UTI in their lifetime [8]. E. coli is the most common cause of community-acquired UTI and is responsible for more than 80% of the cases [9].
UTI diagnostics involves multiple steps, integrating various techniques and equipment. Usually, after the initial patient examination, a dipstick urinalysis is performed,

Chip Design and Production
Three microfluidic chips (Chip #1, Chip #2, and Chip #3) were designed using Autodesk Tinkercad and SolidWorks software ( Figure 1C). Chip #1 has a single channel and is used for bacteria detection. Chip #2 and Chip #3 are multi-channel chips for the AST. For initial prototyping, the 3D chip model was sliced using Ultimaker Cura 4.5 software and printed with an Ender printer using a white PLA filament. A centrifuge adapter (Supplemental Materials; Figure S1) and microscope adapter (Supplemental Materials; Figure S2). were printed with an Ender printer, using PETG and PLA, respectively. For experiments involving bacteria cells, the 3D chip model was sliced with GrabCAD and printed with a Stratasys J826 Polyjet printer using VeroClear photopolymer and SUP 706B support. The chip was sonicated for 15 min in 100% ethanol before use.
The superhydrophobic coating for the chip was prepared as described elsewhere, with minor modifications [24]. Briefly, 1% w/w FOTS was mixed with pure ethanol, and the solution was vortexed for 5 min at room temperature. P 25 TiO 2 nanoparticles (13% w/w) were added, and the solution was vortexed for an additional 10 min. The superhydrophobic coating was applied on the chip with a 10 uL Hamilton glass syringe.
The volume of a single channel in Chip #3 used for the AST was 28 uL. Antibiotics were drop-dried into the channels at breakpoint concentration, as indicated by the FDA [25]-8 µg/mL for ampicillin, 4 µg/mL for gentamicin, and 16 µg/mL for amikacin.
Glass or transparent polystyrene sheets were used to close the chip (cap). CF-343 silicon glue (Hercules) was applied using a Hyrel3D HYDRA printer with a 1 mL syringe and a 21G needle to bind and seal between the cap and the chip (Supplemental Materials, Figure S1 and Video S1). The glue was left to dry for 24 h before the chip was used.

Bacteria Detection in 10 mL Sample
E. coli cells were grown overnight in lysogeny broth (LB). Bacterial cell culture density was calculated by OD600 of 1.0 = 8 × 10 8 cells/mL and samples were diluted to the desired densities. To imitate UTI detection, E. coli cells were spiked into urine obtained from a healthy volunteer (10 mL of 5 × 10 3 cells/mL). Urine was injected into a standard 15 mL tube, which was connected to Chip #1, directly inserted upside-down into a benchtop centrifuge (Eppendorf centrifuge 5910 R) without any adapter, and centrifuged for 8 min, at 1900 rpm. Excess liquid from the chip was returned to the 15 mL tube by manually rotating the tube (Supplement files, Video S2). Concentrated cells were observed using an EVOS FL Auto microscope with a 60× objective.

Monitoring Bacterial Reaction to Antibiotics in the Chip
E. coli cells in LB medium (300 uL of 5 × 10 5 cells/mL) were injected directly into Chip #3, briefly centrifuged for 10 s at 800 rpm (Sigma 1-15 centrifuge with 3D-printed adapter: Supplemental materials, Figure S1) to push the liquid into the channels, and then incubated for different durations of time, at 37 • C. Photos of the channels were taken, at various magnifications, using an EVOS FL Auto microscope and a custom adapter (Supplemental Materials, Figure S2). Scheme of the proposed device that combines a centrifuge to push the cells into the channels, a microscope to assess bacterial cell morphology and growth, and an incubator to maintain optimal temperature for bacteria growth. (B) A scheme of the proposed UTI diagnosis process, medium exchange, and AST using the BactoSpin system. (C) A photo of 3D-printed BactoSpin chips for UTI diagnostics and AST. Chip #1 was designed to concentrate bacteria from urine samples into a single chamber for UTI diagnostics, cells count and medium exchange. Chip #2 is a 16channel version of the chip designed to perform the AST and Chip #3 is a 4-channel version. (D) Schematic presentation of areas coated with a hydrophilic (green dots) and with a superhydrophobic (red lines) coating. (E) During centrifugation, medium with the bacteria cells is forced into the channels. After centrifugation stops and due to applied coatings, the excess liquid flows back, enabling separation between the channels. (F) A photo of the chip with superhydrophobic and hydrophilic coatings (left) and without coatings (right).

System Design
This work aimed to develop a system that can perform most of the steps necessary for UTI diagnostics and AST in a single machine. As mentioned above, currently, most clinical laboratories rely on an overnight culture of urine samples to obtain a definitive indication of the presence of bacteria and CFU count. There are no consensus criteria device that combines a centrifuge to push the cells into the channels, a microscope to assess bacterial cell morphology and growth, and an incubator to maintain optimal temperature for bacteria growth. (B) A scheme of the proposed UTI diagnosis process, medium exchange, and AST using the BactoSpin system. (C) A photo of 3D-printed BactoSpin chips for UTI diagnostics and AST. Chip #1 was designed to concentrate bacteria from urine samples into a single chamber for UTI diagnostics, cells count and medium exchange. Chip #2 is a 16-channel version of the chip designed to perform the AST and Chip #3 is a 4-channel version. (D) Schematic presentation of areas coated with a hydrophilic (green dots) and with a superhydrophobic (red lines) coating. (E) During centrifugation, medium with the bacteria cells is forced into the channels. After centrifugation stops and due to applied coatings, the excess liquid flows back, enabling separation between the channels. (F) A photo of the chip with superhydrophobic and hydrophilic coatings (left) and without coatings (right).

Machine Learning for AST
E. coli cells (5 × 10 5 cells/mL) were incubated in 24-well plates (Corning ® ) and photographed every 30 min, with an EVOS FL Auto microscope, using a 20× objective. Each photo was split into 100 images using Python. Some wells were randomly selected to

Human Urine Sample
A human urine sample collected from a volunteer UTI patient was obtained from Hadassah Medical Center (Jerusalem, Israel). The experiment was approved by the Ethics Committee of Hadassah University Hospital (IRB number 04-2021). The sample was transferred on ice to the laboratory and was passed through a 5-micron PES syringe filter. Next, the sample was introduced to an LB agar petri dish and incubated overnight under 37 • C. A single colony was selected and incubated for an additional 12 h in liquid LB. Bacteria were then diluted to a concentration of 5 × 10 5 bacteria cells/mL and were injected into Chip #3 containing drop-dried antibiotics. The chip was mounted into the centrifuge using a customized adapter and briefly centrifuged for 10 s, at 800 rpm, as described above. The chip was then transferred to the incubator and incubated for 2 h and for 4 h before being imaged under the EVOS FL Auto microscope.

System Design
This work aimed to develop a system that can perform most of the steps necessary for UTI diagnostics and AST in a single machine. As mentioned above, currently, most clinical laboratories rely on an overnight culture of urine samples to obtain a definitive indication of the presence of bacteria and CFU count. There are no consensus criteria regarding the minimal CFU defining UTI, and it varies greatly across laboratories, with 10 3 CFU being the smallest reported count used to define UTI [27]. After obtaining a count, bacteria are purified from the urine-as urine may have factors that will interfere with the AST, for example, in the case when the patient is already taking an antibiotic treatment. In the next step, the concentration of bacteria cells must be adjusted to that required for the AST assay (5 × 10 5 cells/mL). The next and final step is AST itself.
The current work presents the concept of a device ( Figure 1A-E) that combines a microscope, a centrifuge, an incubator, and microfluidic chips for the semiautomatic processing of urine samples suspected to be carrying infectious bacteria.
Chip #1 ( Figure 1C) was used for bacteria detection and was designed with an adaptor, which acts as a cap for standard 15 mL tubes. On the top side of the chip, a single triangular channel, 50 microns in height, was used for cell concentration and observation. E. coli cells (5 × 10 3 ) were spiked into 10 mL human urine (prefiltered through a 5-micron syringe filter) obtained from a healthy volunteer in a 15 mL tube, which was then inserted into a centrifuge with the chip pointing down. The sample was centrifuged for 8 min. The chip was designed to enable easy flow of the medium to the end of the chip during centrifugation and easy flow back to the tube when the tube is taken out of the centrifuge (Supplemental Files; Video S2). The concentrated bacteria became trapped in the channel, as observed under a microscope ( Figure 2). This step enabled easy bacteria detection in under 10 min and effectively concentrated samples with as few as 5 × 10 3 cells/mL. While in the current work, the concentrated cells were observed and manually detected, for the future, it has been proposed to use image recognition software that will enable automatic bacteria detection and counting.
After initial detection, bacterial cells must be transferred from urine to a suitable medium for further growth. This can be achieved by connecting a fresh tube to the chip and inverting the complex, such that the chip points upward ( Figure 1B, Steps 2 and 3). Brief centrifugation should bring the bacterial cells trapped in the chamber down to the new tube. Fresh dedicated growth medium can be added to the tube before centrifugation. After centrifugation, the final concentration can be adjusted as needed. Then, the tube with the bacteria is connected to Chip #2 for the AST assay ( Figure 1B, Step 4) and briefly centrifuged to bring the medium with the cells to the channels of Chip #2 containing dried antibiotics. In the current work, due to extensive leakage from the 3D-printed prototype (caused by a poor fit of the chip to the thread on a standard 15 mL tube) and safety reasons, 5 × 10 5 bacterial cells in LB were directly injected into a simplified Chip #3 ( Figure 1C) to demonstrate AST. E. coli cells (5 × 10 3 ) were spiked into 10 mL human urine (prefiltered through a 5-micron syringe filter) obtained from a healthy volunteer in a 15 mL tube, which was then inserted into a centrifuge with the chip pointing down. The sample was centrifuged for 8 min. The chip was designed to enable easy flow of the medium to the end of the chip during centrifugation and easy flow back to the tube when the tube is taken out of the centrifuge (Supplemental Files; Video S2). The concentrated bacteria became trapped in the channel, as observed under a microscope ( Figure 2). This step enabled easy bacteria detection in under 10 min and effectively concentrated samples with as few as 5 × 10 3 cells/mL. While in the current work, the concentrated cells were observed and manually detected, for the future, it has been proposed to use image recognition software that will enable automatic bacteria detection and counting. After initial detection, bacterial cells must be transferred from urine to a suitable medium for further growth. This can be achieved by connecting a fresh tube to the chip and inverting the complex, such that the chip points upward ( Figure 1B, Steps 2 and 3). Brief centrifugation should bring the bacterial cells trapped in the chamber down to the new tube. Fresh dedicated growth medium can be added to the tube before centrifugation. After centrifugation, the final concentration can be adjusted as needed. Then, the tube with the bacteria is connected to Chip #2 for the AST assay ( Figure 1B, Step 4) and briefly centrifuged to bring the medium with the cells to the channels of Chip #2 containing dried antibiotics. In the current work, due to extensive leakage from the 3D-printed prototype (caused by a poor fit of the chip to the thread on a standard 15 mL tube) and safety reasons, 5 × 10 5 bacterial cells in LB were directly injected into a simplified Chip #3 ( Figure 1C) to demonstrate AST.

Designing a Chip for AST
Growing bacterial cells in isolated wells, each containing different antibiotics at specific concentrations, is a well-established system for AST [28,29]. Here, we propose a chip that contains multiple small channels for bacterial cell incubation. We designed Chip #3 with 4 channels and Chip #2 with 16 channels ( Figure 1C). One channel in each chip serves as a control, while the others contain drop-dried antibiotics at breaking point concentrations [25]. The sample can be introduced via a 15 mL tube ( Figure 1B, Step 4) or injected directly into the chip. Brief centrifugation will force the liquid with the bacterial cells into the channels ( Figure 1E); such liquid would otherwise not enter the channels (Supplemental Materials, Figure S3).
To create isolated environments for testing each antibiotic, there must be a separation between channels. For this purpose, the excess liquid must be removed, and capillary flow between the channels must be prevented. If the chip is used as is, the liquid remains stuck in the upper part of the chip ( Figures 1F and 3A). However, excess liquid can be passively brought back to the bottom part of the chip after centrifugation stops without physically rotating the chip, by designing regions within the chip with different levels of hydrophobicity.

Designing a Chip for AST
Growing bacterial cells in isolated wells, each containing different antibiotics at specific concentrations, is a well-established system for AST [28,29]. Here, we propose a chip that contains multiple small channels for bacterial cell incubation. We designed Chip #3 with 4 channels and Chip #2 with 16 channels ( Figure 1C). One channel in each chip serves as a control, while the others contain drop-dried antibiotics at breaking point concentrations [25]. The sample can be introduced via a 15 mL tube ( Figure 1B, Step 4) or injected directly into the chip. Brief centrifugation will force the liquid with the bacterial cells into the channels ( Figure 1E); such liquid would otherwise not enter the channels (Supplemental Materials, Figure S3).
To create isolated environments for testing each antibiotic, there must be a separation between channels. For this purpose, the excess liquid must be removed, and capillary flow between the channels must be prevented. If the chip is used as is, the liquid remains stuck in the upper part of the chip (Figures 1F and 3A). However, excess liquid can be passively brought back to the bottom part of the chip after centrifugation stops without physically rotating the chip, by designing regions within the chip with different levels of hydrophobicity.
First, to enhance backflow of the excess liquid to the main reservoir, we coated the lower part of the chip with a silicone surfactant BYK-348. BYK-348 significantly reduces the water contact angle, thus drawing the liquid back. However, despite some improvement ( Figure 3B), part of the liquid still remained and connected between the channels driven by a capillary force (Supplemental Materials Figure S4).
To try to enhance separation between the channels, the top part of the chip was treated with a superhydrophobic coating. Surface coated with superhydrophobic paint based on titanium FOTS-coated dioxide nanoparticles (with a particle size of 21 nm), a combination which creates a lotus effect [24,30], exhibited a higher water contact angle compared to uncoated surface (Supplemental Materials Figure S5). However, while the coating effectively separated the middle channels, superhydrophobic coating alone was insufficient to fully isolate the side channels ( Figure 3C). Thus, a combination of both hydrophilic coating and superhydrophobic coating was applied. This approach enabled full and spontaneous backflow of the medium and effective complete separation between the channels in both Chip #2 and Chip #3 (Figures 1F  and 3D; Supplemental materials: Video 3, 4, 5 and 6). A green food color drop-dried in the coated channels remained in the channel, even after a 24 h incubation ( Figures 4D-F and  S6A), while in the uncoated chip, the color diffused out within less than 30 min ( Figures  4A-C and S6B), indicating efficient separation of the channels when the coating is applied.  Figure 3B), part of the liquid still remained and connected between the channels driven by a capillary force (Supplemental Materials Figure S4).
To try to enhance separation between the channels, the top part of the chip was treated with a superhydrophobic coating. Surface coated with superhydrophobic paint based on titanium FOTS-coated dioxide nanoparticles (with a particle size of 21 nm), a combination which creates a lotus effect [24,30], exhibited a higher water contact angle compared to uncoated surface (Supplemental Materials Figure S5). However, while the coating effectively separated the middle channels, superhydrophobic coating alone was insufficient to fully isolate the side channels ( Figure 3C).
Thus, a combination of both hydrophilic coating and superhydrophobic coating was applied. This approach enabled full and spontaneous backflow of the medium and effective complete separation between the channels in both Chip #2 and Chip #3 (Figures 1F and 3D; Supplemental materials: Videos S3-S6). A green food color drop-dried in the coated channels remained in the channel, even after a 24 h incubation ( Figures 4D-F and S6A), while in the uncoated chip, the color diffused out within less than 30 min (Figures 4A-C and S6B), indicating efficient separation of the channels when the coating is applied. Moreover, these results also propose that dried antibiotics inside the channels will not have enough time to diffuse out of the channels during the short centrifugation stage.

In-Chip AST of E. coli Cells
To demonstrate AST, we used the four-channel chip with superhydrophobic and hydrophilic coatings. One channel served as a control, while three others held drop-dried ampicillin, gentamicin and amikacin. We designed and printed an adaptor to hold the chip in a standard benchtop centrifuge (Supplemental Files; Figure S7). After coatings, the chip was closed with a glass lid using silicone glue. E. coli cells (5 × 10 5 ) in LB medium were injected into the chip via a small hole in the back, briefly centrifuged, and were incubated for 2 or 4 h. As shown in Figures 5 and S8, a clear difference in cell counts was observed between the control channel and channels containing drop-dried antibiotics, indicating their susceptibility to tested antibiotics. While after 2 h, the difference was only discernible using high magnification ( Figure 5A-D), after 4 h of incubation, the differences in cell numbers were clearly seen even with a low-magnification objective (Supplemental Figure S8). The fact that E. coli cells grew extensively in the control channel indicated that the superhydrophobic and hydrophilic coatings prevented the antibiotics from diffusing out of the test channels to the control channel. The antibiotics efficiently diffused inside the test channels, as evidenced by the absence of bacterial growth along the length of the channels that contained antibiotics.

In-Chip AST of E. coli Cells
To demonstrate AST, we used the four-channel chip with superhydrophobic and hydrophilic coatings. One channel served as a control, while three others held drop-dried ampicillin, gentamicin and amikacin. We designed and printed an adaptor to hold the chip in a standard benchtop centrifuge (Supplemental Files; Figure S7). After coatings, the chip was closed with a glass lid using silicone glue. E. coli cells (5 × 10 5 ) in LB medium were injected into the chip via a small hole in the back, briefly centrifuged, and were incubated for 2 or 4 h. As shown in Figures 5 and S8, a clear difference in cell counts was observed between the control channel and channels containing drop-dried antibiotics, indicating their susceptibility to tested antibiotics. While after 2 h, the difference was only discernible using high magnification ( Figure 5A-D), after 4 h of incubation, the differences in cell numbers were clearly seen even with a low-magnification objective (Supplemental Figure S8). The fact that E. coli cells grew extensively in the control channel indicated that the superhydrophobic and hydrophilic coatings prevented the antibiotics from diffusing out of the test channels to the control channel. The antibiotics efficiently diffused inside the test channels, as evidenced by the absence of bacterial growth along the length of the channels that contained antibiotics.

In-Chip AST of Bacteria Isolated from a Clinical Urine Sample
To further demonstrate the feasibility of the BactoSpin system, AST was performed using bacteria isolated from a urine sample collected from a UTI patient. The bacteria in the urine sample had been identified as Klebsiella pneumoniae by the hospital laboratory. Incubation of the bacteria in the chip containing antibiotics, for 2 h (Figure 6) or 4 h (Supplemental Files; Figure S9), demonstrated resistance to ampicillin and gentamicin, but susceptibility to amikacin. These results were in correlation with the results obtained by the hospital laboratory.

In-Chip AST of Bacteria Isolated from a Clinical Urine Sample
To further demonstrate the feasibility of the BactoSpin system, AST was performed using bacteria isolated from a urine sample collected from a UTI patient. The bacteria in the urine sample had been identified as Klebsiella pneumoniae by the hospital laboratory. Incubation of the bacteria in the chip containing antibiotics, for 2 h (Figure 6) or 4 h (Supplemental Files; Figure S9), demonstrated resistance to ampicillin and gentamicin, but susceptibility to amikacin. These results were in correlation with the results obtained by the hospital laboratory.

In-Chip AST of Bacteria Isolated from a Clinical Urine Sample
To further demonstrate the feasibility of the BactoSpin system, AST was performed using bacteria isolated from a urine sample collected from a UTI patient. The bacteria in the urine sample had been identified as Klebsiella pneumoniae by the hospital laboratory. Incubation of the bacteria in the chip containing antibiotics, for 2 h (Figure 6) or 4 h (Supplemental Files; Figure S9), demonstrated resistance to ampicillin and gentamicin, but susceptibility to amikacin. These results were in correlation with the results obtained by the hospital laboratory.

Application of Machine Learning for Classification of Microscopic Images of Bacterial Cells for AST
The images from the AST discussed above were manually analyzed. However, deep learning models can be applied to process visual data. To demonstrate the possibility of using machine learning (ML) for AST analysis, E. coli cells were incubated in LB inside a 48-well plate containing drop-dried gentamicin. The wells were photographed 30, 60 and 90 min post-seeding ( Figure 7A,B). The images were used to train a residual neural network (ResNet). E. coli cells were also counted manually at the same time points. The manual count found no significant differences in cell numbers after 30 min and after 60 min while it did record a significant difference after 90 min of incubation ( Figure 7C). The ML model found no differences in cell counts after 30 min, but significantly improved after 60 min, yielding 70% accuracy. The accuracy increased to 98% after an additional 30 min of incubation ( Figure 7D). These results demonstrate that ML can both provide accurate classification after 90 min of incubation, a time point at which differences in cell numbers were visibly detectable, and also detect some other changes that are not based on cell number at least 30 min before the differences in cell numbers became visible. One of the observed changes is the cells' length ( Figure 8).

Application of Machine Learning for Classification of Microscopic Images of Bacterial Cells for AST
The images from the AST discussed above were manually analyzed. However, deep learning models can be applied to process visual data. To demonstrate the possibility of using machine learning (ML) for AST analysis, E. coli cells were incubated in LB inside a 48-well plate containing drop-dried gentamicin. The wells were photographed 30, 60 and 90 min post-seeding ( Figure 7A,B). The images were used to train a residual neural network (ResNet). E. coli cells were also counted manually at the same time points. The manual count found no significant differences in cell numbers after 30 min and after 60 min while it did record a significant difference after 90 min of incubation ( Figure 7C). The ML model found no differences in cell counts after 30 min, but significantly improved after 60 min, yielding 70% accuracy. The accuracy increased to 98% after an additional 30 min of incubation ( Figure 7D). These results demonstrate that ML can both provide accurate classification after 90 min of incubation, a time point at which differences in cell numbers were visibly detectable, and also detect some other changes that are not based on cell number at least 30 min before the differences in cell numbers became visible. One of the observed changes is the cells' length ( Figure 8).

Conclusions
In summary, this work presented a proof-of-concept study of a device for rapid UTI diagnostic and AST. For the UTI diagnostics, a single-channel microfluidic chip capable of detecting 5 × 10 3 cells/mL, a clinically relevant concentration of bacterial cells in urine, within 10 min, was developed. The same device can also be used for AST when equipped with a 4-or 16-channel chip. Application of both hydrophilic and superhydrophobic coatings to the chip's surface enabled separation between the test zone and the sample-receiving zone as well as between the channels themselves. Rapid (2 h), in-chip AST of E. coli, as well of K. pneumoniae that was isolated from urine of a UTI patient, was demonstrated. Using a well plate, it was demonstrated that deep learning could accelerate the process, providing results within 60 min, with an accuracy of 70%. After 90 min of incubation, the accuracy increases to 98%. The presented prototypes can be further developed as a platform for efficient and rapid UTI diagnostics and AST.
Supplementary Materials: The following are available online at www.mdpi.com/xxx/s1, Video S1: Silicon glue deposition on a glass cover. Video S2: Fully assembled Chip #1 attached to a 15 mL tube. Video 3 and Video 6: Demonstration of the spontaneous flow-back of liquid and channel separation in a chip with hydrophilic and hydrophobic coatings. Video S4 and S5: Demonstration of absence of spontaneous flow-back of liquid in a chip without hydrophilic and hydrophobic coatings. Figure  S1: Chip assembling process. Figure S2: An EVOS microscope adaptor to hold the chip. Figure S3: Demonstration of lack of spontaneous liquid flow into the channels of the chip. Figure S4: Flow between two channels when no superhydrophobic coating is applied. Figure S5: Demonstration of titanium dioxide-based superhydrophobic coating. Figure S6: The green dye does not diffuse out of the microchannel. Figure S7: A custom-made adapter for a standard table centrifuge. Figure S8: Brightfield microscopy photos of E. coli cells incubated for 4 h inside microchannels in a chip with or without addition of antibiotics. Figure S9: Klebsiella pneumoniae isolated from a clinical urine sample incubated for 4 h in chip with or without addition of antibiotics.

Conclusions
In summary, this work presented a proof-of-concept study of a device for rapid UTI diagnostic and AST. For the UTI diagnostics, a single-channel microfluidic chip capable of detecting 5 × 10 3 cells/mL, a clinically relevant concentration of bacterial cells in urine, within 10 min, was developed. The same device can also be used for AST when equipped with a 4-or 16-channel chip. Application of both hydrophilic and superhydrophobic coatings to the chip's surface enabled separation between the test zone and the samplereceiving zone as well as between the channels themselves. Rapid (2 h), in-chip AST of E. coli, as well of K. pneumoniae that was isolated from urine of a UTI patient, was demonstrated. Using a well plate, it was demonstrated that deep learning could accelerate the process, providing results within 60 min, with an accuracy of 70%. After 90 min of incubation, the accuracy increases to 98%. The presented prototypes can be further developed as a platform for efficient and rapid UTI diagnostics and AST.
Supplementary Materials: The following are available online at https://www.mdpi.com/article/10 .3390/s21175902/s1, Video S1: Silicon glue deposition on a glass cover. Video S2: Fully assembled Chip #1 attached to a 15 mL tube. Videos S3 and S6: Demonstration of the spontaneous flow-back of liquid and channel separation in a chip with hydrophilic and hydrophobic coatings. Videos S4 and S5: Demonstration of absence of spontaneous flow-back of liquid in a chip without hydrophilic and hydrophobic coatings. Figure S1: Chip assembling process. Figure S2: An EVOS microscope adaptor to hold the chip. Figure S3: Demonstration of lack of spontaneous liquid flow into the channels of the chip. Figure S4: Flow between two channels when no superhydrophobic coating is applied. Figure S5: Demonstration of titanium dioxide-based superhydrophobic coating. Figure S6: The green dye does not diffuse out of the microchannel. Figure S7: A custom-made adapter for a standard table centrifuge. Figure S8: Brightfield microscopy photos of E. coli cells incubated for 4 h inside microchannels in a chip with or without addition of antibiotics. Figure S9: Klebsiella pneumoniae isolated from a clinical urine sample incubated for 4 h in chip with or without addition of antibiotics.