Ammonia Gas Sensor Based on Graphene Oxide-Coated Mach-Zehnder Interferometer with Hybrid Fiber Structure

A graphene oxide-coated in-fiber Mach-Zehnder interferometer (MZI) formed with a multimode fiber-thin core fiber-multimode fiber (MMF-TCF-MMF) is proposed and experimentally demonstrated for ammonia gas (NH3) sensing. The MZI structure is composed of two segments of MMF of length 2 mm, with a flame-tapered TCF between them as the sensing arm. The MMFs act as mode couplers to split and recombine light owing to the core diameter mismatch with the other fibers. A tapered TCF is formed by the flame melting taper method, resulting in evanescent wave leakage. A layer of graphene oxide (GO) is applied to the tapered region of the TCF to achieve gas adsorption. The sensor operates on the principle of changing the effective refractive index of the cladding mode of a fiber through changing the conductivity of the GO coating by adsorbed NH3 molecules, which gives rise to a phase shift and shows as the resonant dip shifts in the transmission spectrum. So the concentration of the ammonia gas can be obtained by measuring the dip shift. A wavelength-shift sensitivity of 4.97 pm/ppm with a linear fit coefficient of 98.9% is achieved for ammonia gas concentrations in the range of 0 to 151 ppm. In addition, we performed a repetitive dynamic response test on the sensor by charging/releasing NH3 at concentration of 200 ppm and a relative humidity test in a relative humidity range of 35% to 70%, which demonstrates the reusability and stability of the sensor.


Introduction
Toxic, flammable and explosive gases are often found in people's daily lives and production activities, which have major safety accidents. Volatile and toxic ammonia gas is an important component in industrial production [1]. Once a leak occurs, ammonia gas can cause environmental pollution and pose a serious health risk, such as concentrations over 100 ppm rapidly induce irritations of eyes and respiratory tracts and create functional troubles. Therefore, the detection and early warning of ammonia gas is necessary. Since ammonia gas applications are usually in a harsh environment such as corrosive, traditional electrochemical sensors are widely used due to their high measurement accuracy [2][3][4][5], but have many shortcomings such as short life, easy contamination and poor repeatability. A sensor capable of overcoming the above disadvantages and capable of performing timely, effective and stable ammonia gas detection is undoubtedly a research hotspot. Since the advent of fiber-optic sensors, they have attracted much attention. Compared with traditional sensing technologies, fiber-optic sensing technologies have characteristics of simple structure, high sensing sensitivity, small size, anti-electromagnetic interference and distributed online monitoring. As a result, more and more fiber-optic-based ammonia gas sensor have appeared in recent years. According to the principle of sensing, there are sensors based on fiber evanescent fields [6], surface plasmon resonance [7] and Bragg grating [8,9]. Various special types of fibers are also used to make related sensors, such as porous plastic fiber [10], Figure 1a shows the structure of an optical fiber MZI sensor, in which a tapered-TCF is spliced between two MMFs (MMF1 and MMF2) to act as the sensing arm; the MMFs are spliced to the single-mode fiber (SMF1 and SMF2) which play the role as the mode couplers. When light from SMF1 is launched through MMF1, due to the mismatch of the mode field diameter, some is coupled into the cladding of TCF and excites different cladding modes while the remaining light enters the core of the TCF and continues to transmit. In the tapered region of TCF, part of the light in the core and cladding will leak to the external environment in the form of evanescent waves due to the reduction of the fiber diameter. This will cause the tapered area to be very sensitive to the changes of external environment. At the TCF-MMF2 splice point, the cladding modes of the TCF are coupled into the MMF2 core and interfere with each other as well as with the core mode. Subsequently, most of the light are coupled into the core of SMF2. Figure 1b shows the simulated result with the software of COMSOL Multiphysics. It can be found that the beam splitting and coupling occur at the regions of MMFs. 2. The fabrication of the sensing head is presented in Section 3. Section 4 contains the experimental results and discussions. Finally, the paper is ended with some conclusion in Section 5. Figure 1a shows the structure of an optical fiber MZI sensor, in which a tapered-TCF is spliced between two MMFs (MMF1 and MMF2) to act as the sensing arm; the MMFs are spliced to the single-mode fiber (SMF1 and SMF2) which play the role as the mode couplers. When light from SMF1 is launched through MMF1, due to the mismatch of the mode field diameter, some is coupled into the cladding of TCF and excites different cladding modes while the remaining light enters the core of the TCF and continues to transmit. In the tapered region of TCF, part of the light in the core and cladding will leak to the external environment in the form of evanescent waves due to the reduction of the fiber diameter. This will cause the tapered area to be very sensitive to the changes of external environment. At the TCF-MMF2 splice point, the cladding modes of the TCF are coupled into the MMF2 core and interfere with each other as well as with the core mode. Subsequently, most of the light are coupled into the core of SMF2. Figure 1b shows the simulated result with the software of COMSOL Multiphysics. It can be found that the beam splitting and coupling occur at the regions of MMFs. In this paper, we use the MMFs to adjust the number and intensity of excitation modes and the intensity ratio between core mode and cladding modes. However, the MMFs will also cause additional phase differences between the core mode and the cladding modes because the transmission path of them is not exactly same. Thus, the length of the two MMFs should be suitably selected so that the phase difference between their guided modes can be negligible and the cladding modes can be excited sufficiently. The interference is mainly formed by two modes (the core mode and the dominant cladding mode) in the sensor, so the structure of sensor still can be considered as a Mach-Zehnder interferometer. The output intensity and phase difference can be expressed as follows:

Sensor Structure and Principle
where: In this paper, we use the MMFs to adjust the number and intensity of excitation modes and the intensity ratio between core mode and cladding modes. However, the MMFs will also cause additional phase differences between the core mode and the cladding modes because the transmission path of them is not exactly same. Thus, the length of the two MMFs should be suitably selected so that the phase difference between their guided modes can be negligible and the cladding modes can be excited sufficiently. The interference is mainly formed by two modes (the core mode and the dominant cladding mode) in the sensor, so the structure of sensor still can be considered as a Mach-Zehnder interferometer. The output intensity and phase difference can be expressed as follows: where: I co and I cl represent the light intensity of the core and cladding mode, respectively, and ϕ is the phase difference of them. The incident wavelength is λ and L is the length of the tapered-TCF. The effective refractive indexes of the TCF core and cladding mode are n co and n cl , respectively, while ∆n e f f is the difference between them. When the phase difference Sensors 2021, 21, 3886 4 of 13 ϕ = (2m + 1) π in (1) and m is an integer, the intensity of interference reaches a minimum value and the wavelength of the interference valley in the transmission spectrum is: From (3), one can see that the wavelength of the interference valley λ dip will vary with a change in ∆n e f f or L. In this paper, a GO film is coated on the surface of the tapered TCF. When the GO absorbs the ammonia gas, the refractive index of it will vary due to the conductivity changing. As a result, the n cl and ∆n e f f will change and the λ dip will shift. So, the concentration of ammonia gas can be measured by the shift of λ dip .

Fusion and Tapering of the Sensor
As shown in Figure 1, the fibers are spliced in turn with a fusion splicer (FITEL S178, Furukawa Electric, Tokyo, Japan). The arc power and duration time are 140 bit and 3000 ms, respectively. The diameters of the core/cladding of the SMF, MMF and TCF were 9/125 µm, 105/125 µm and 6.4/125 µm, respectively. It should be noted that the MMFs act as mode couplers, and their length affects the excitation and coupling efficiency of the cladding modes. A short MMF cannot excite the cladding mode sufficiently and the coupling efficiency is low. Through repeated attempts and experiments, the length of the two MMFs of 2 mm was selected to obtain a good interference pattern. As the sensing arm, the length of the TCF will affect the free spectral range (FSR) of the interference spectrum. In general, the longer the length of the TCF, the narrower the FSR that can be achieved. Figure 2a shows the transmission spectra for the TCF lengths of 30 and 50 mm, in which the FSR is seen to decrease as the length increases. The inhomogeneous interference fringes also show that several cladding modes have been excited, as shown in Figure 2b. Figure 2b is the Fourier transform from Figure 2a, which can show the spatial frequency characteristics [42][43][44]. We believe that the interference is from the TCF and the spectrum will be affected by the ambient conditions of the TCF. In this paper, the L = 30 mm is used for the taper and ammonia gas response experiment.   In order to create better coupling into the cladding mode, the TCF is stretched to introduce a taper. The sensing head is fixed on the three-dimensional adjustment frame and the TCF is heated by a torch (PT-220, IRODA, Taiwan) and stretched to obtain a tapered-TCF. Figure 3a shows an SEM image of the TCF after tapering. One can see that the cladding diameter of the tapered TCF has decreased from 125 µm to about 80 µm. The transmission spectrum of the sensor with and without tapering is shown in Figure 3b. After tapered, the contrast ratio increases because the intensity ratio between the core mode and cladding mode varies, meanwhile the dip wavelengths are almost unchanged.

Preparation and Coating of Graphene Oxide
The process of coating GO onto the tapered-TCF is shown in Figure 4. First, a 0.08 mg/mL GO dispersion (XF020, Nanjing XFNANO Materials Tech. Co., Ltd., Nanjing China) was centrifuged at 10,000 RPM for 15 min to create GO sheets with a size larger than 500 nm. Second, the fiber was cleaned with alcohol several times, and the sensing area was immersed in a sufficient-sized droplet of GO dispersion and was naturally evap orated for 24 h at temperature of 25 °C. As a result, GO adhered to the surface of the fiber seen in the SEM image ( Figure 5). We observe that the GO layer is uniformly attached to the surface of the tapered-TCF and a large number of undulating folds are formed simul taneously. The undulating folds increase the effective surface area of the GO exposed to the gas, which is advantageous for absorption. It should be noted that the thickness of the GO film can be adjusted by controlling the concentration of the GO solution and the im mersion time. For comparison, we measured the transmission spectra of the sensor with and without GO ( Figure 6). There are no obvious changes except for the contrast, and the GO coating has not affected the interference properties of the sensor.

Preparation and Coating of Graphene Oxide
The process of coating GO onto the tapered-TCF is shown in Figure 4. First, a 0.08 mg/mL GO dispersion (XF020, Nanjing XFNANO Materials Tech. Co., Ltd., Nanjing, China) was centrifuged at 10,000 RPM for 15 min to create GO sheets with a size larger than 500 nm. Second, the fiber was cleaned with alcohol several times, and the sensing area was immersed in a sufficient-sized droplet of GO dispersion and was naturally evaporated for 24 h at temperature of 25 • C. As a result, GO adhered to the surface of the fiber, seen in the SEM image ( Figure 5). We observe that the GO layer is uniformly attached to the surface of the tapered-TCF and a large number of undulating folds are formed simultaneously. The undulating folds increase the effective surface area of the GO exposed to the gas, which is advantageous for absorption. It should be noted that the thickness of the GO film can be adjusted by controlling the concentration of the GO solution and the immersion time. For comparison, we measured the transmission spectra of the sensor with and without GO ( Figure 6). There are no obvious changes except for the contrast, and the GO coating has not affected the interference properties of the sensor.     To further determine the elemental composition of the GO coating material on the fiber, we performed an energy dispersive spectroscopy (EDS) analysis, as shown in Figure 7a. The coating material mainly contains the elements C, O, Si and Pt; Si is a component of the optical fiber, and Pt was sprayed on to obtain the EDS image. Figure 7b shows the atomic composition of each major element of the coating material; the specific gravity of the three elements C, O and Si are 59.92, 38.17 and 1.92%, respectively, and the GO coating is without significant impurities. It should be noted that the functional group in the GO contains hydrogen, which does not appear in this EDS elemental analysis.    To further determine the elemental composition of the GO coating material on the fiber, we performed an energy dispersive spectroscopy (EDS) analysis, as shown in Figure 7a. The coating material mainly contains the elements C, O, Si and Pt; Si is a component of the optical fiber, and Pt was sprayed on to obtain the EDS image. Figure 7b shows the atomic composition of each major element of the coating material; the specific gravity of the three elements C, O and Si are 59.92, 38.17 and 1.92%, respectively, and the GO coating is without significant impurities. It should be noted that the functional group in the GO contains hydrogen, which does not appear in this EDS elemental analysis. To further determine the elemental composition of the GO coating material on the fiber, we performed an energy dispersive spectroscopy (EDS) analysis, as shown in Figure 7a. The coating material mainly contains the elements C, O, Si and Pt; Si is a component of the optical fiber, and Pt was sprayed on to obtain the EDS image. Figure 7b shows the atomic composition of each major element of the coating material; the specific gravity of the three elements C, O and Si are 59.92, 38.17 and 1.92%, respectively, and the GO coating is without significant impurities. It should be noted that the functional group in the GO contains hydrogen, which does not appear in this EDS elemental analysis.

Experimental Results and Discussion
The performance of this sensor was investigated using the experimental setup shown in Figure 8. The sensor was placed in a sealed gas chamber. A broadband light source

Experimental Results and Discussion
The performance of this sensor was investigated using the experimental setup shown in Figure 8. The sensor was placed in a sealed gas chamber. A broadband light source (BBS) (ASE-C+L module, HOYATEK, Shenzhen, China) was used to illuminate the interferometer. The output spectrum was acquired by an optical spectrum analyzer (OSA) (AQ6370B, YOKOGAWA, Tokyo, Japan). The gas chamber was constructed of pure silica and kept sealed; the inlet and outlet ports were gas-tight switched valves. Before injecting ammonia gas, the air chamber was connected with the ventilation unit for one hour to discharge as much water vapor and other impurities as possible from the air chamber and the pipeline. The outlet valve was then closed to ensure that the air in the gas chamber was no longer disturbed by external ambient air. At this time, there was only air in the gas chamber, and the output spectrum was recorded as an initial reference for the NH 3 concentration 0 ppm. Subsequently, the mixture of NH 3 and N 2 in a volume ratio of 5:95 was gradually injected into the gas chamber to mix with the air to get different NH 3 concentrations. The main component of air retained in the air chamber is nitrogen gas, and only nitrogen gas follows a trace amount of ammonia gas into the gas chamber. Further, the molar amount of the injected gas is also small due to the low NH 3 concentration. So, it can be considered that the change of the transmission spectrum in the experiments is caused by the concentration adjustment of the ammonia gas.
in Figure 8. The sensor was placed in a sealed gas chamber. (BBS) (ASE-C+L module, HOYATEK, Shenzhen, China) was u ferometer. The output spectrum was acquired by an optical (AQ6370B, YOKOGAWA, Tokyo, Japan). The gas chamber wa and kept sealed; the inlet and outlet ports were gas-tight switch ammonia gas, the air chamber was connected with the ventil discharge as much water vapor and other impurities as possibl the pipeline. The outlet valve was then closed to ensure that was no longer disturbed by external ambient air. At this time, t chamber, and the output spectrum was recorded as an initial centration 0 ppm. Subsequently, the mixture of NH3 and N2 in gradually injected into the gas chamber to mix with the air to trations. The main component of air retained in the air chambe nitrogen gas follows a trace amount of ammonia gas into the molar amount of the injected gas is also small due to the low N be considered that the change of the transmission spectrum in by the concentration adjustment of the ammonia gas. In the experiments, the ammonia gas concentration increa ure 9 shows the transmission spectra for different concentrati gas mass flow rate into the chamber was precisely controlled b and the gas concentration was monitored in real time using a c sor placed in the chamber to achieve different ammonia gas obtain good observation results, we selected the resonant dip n In the experiments, the ammonia gas concentration increased from 0 to 151 ppm. Figure 9 shows the transmission spectra for different concentrations of NH 3 . The ammonia gas mass flow rate into the chamber was precisely controlled by a digital mass flow meter and the gas concentration was monitored in real time using a chemical ammonia gas sensor placed in the chamber to achieve different ammonia gas concentrations. In order to obtain good observation results, we selected the resonant dip near 1587 nm in Figure 6 as the observation point, and recorded the output spectrum for different ammonia gas concentrations. After the end of the experiment, the gas was discharged from the gas chamber. All experiments were carried out at a temperature of 25 • C and the pressure inside the gas chamber was the same as the atmospheric pressure. the observation point, and recorded the output spectrum for different amm centrations. After the end of the experiment, the gas was discharged from ber. All experiments were carried out at a temperature of 25 °C and the p the gas chamber was the same as the atmospheric pressure. From Figure 9, we can see that the wavelength of the resonant dip sh wavelengths as the concentration of NH3 increases through the range 0-15 are many polar functional groups in GO, such as hydroxyl and carboxy epoxy functional groups, which can promote the adsorption of NH3 on th sorbs NH3 and interacts with NH3, and the charge transfer from NH3 to GO icant due to the formation of surface hydrogen bonds [37,39]. The above pro in a decrease in the dielectric constant and conductivity of GO, so the refra GO will increase because it is inverse proportion to the dielectric constant a ity, as shown in Figure 10, and then the effective refractive index of the c will increase due to the effect by the refractive index change of the GO. A wavelength of the resonant dip will shift to short wavelength because the e tive index difference between the core mode and the cladding mode decrea periment, the absorption effect will increase when the ammonia gas con creases. The phase difference between the core mode and the cladding mod further, so the wavelength of the resonant dip will continually shift to shor On the contrary, the wavelength of the resonant dip will shift to near the or when NH3 is desorbed from the GO. From Figure 9, we can see that the wavelength of the resonant dip shifts to shorter wavelengths as the concentration of NH 3 increases through the range 0-151 ppm. There are many polar functional groups in GO, such as hydroxyl and carboxyl groups, and epoxy functional groups, which can promote the adsorption of NH 3 on the GO. GO absorbs NH 3 and interacts with NH 3 , and the charge transfer from NH 3 to GO is very significant due to the formation of surface hydrogen bonds [37,39]. The above process will result in a decrease in the dielectric constant and conductivity of GO, so the refractive index of GO will increase because it is inverse proportion to the dielectric constant and conductivity, as shown in Figure 10, and then the effective refractive index of the cladding mode will increase due to the effect by the refractive index change of the GO. As a result, the wavelength of the resonant dip will shift to short wavelength because the effective refractive index difference between the core mode and the cladding mode decreases. In the experiment, the absorption effect will increase when the ammonia gas concentration increases. The phase difference between the core mode and the cladding mode will decrease further, so the wavelength of the resonant dip will continually shift to short wavelength. On the contrary, the wavelength of the resonant dip will shift to near the original position when NH 3 is desorbed from the GO. From Figure 9, we can see that the wavelength of the resonant dip s wavelengths as the concentration of NH3 increases through the range 0-1 are many polar functional groups in GO, such as hydroxyl and carbox epoxy functional groups, which can promote the adsorption of NH3 on t sorbs NH3 and interacts with NH3, and the charge transfer from NH3 to GO icant due to the formation of surface hydrogen bonds [37,39]. The above pro in a decrease in the dielectric constant and conductivity of GO, so the refr GO will increase because it is inverse proportion to the dielectric constant ity, as shown in Figure 10, and then the effective refractive index of the will increase due to the effect by the refractive index change of the GO. wavelength of the resonant dip will shift to short wavelength because the tive index difference between the core mode and the cladding mode decre periment, the absorption effect will increase when the ammonia gas co creases. The phase difference between the core mode and the cladding mod further, so the wavelength of the resonant dip will continually shift to sho On the contrary, the wavelength of the resonant dip will shift to near the o when NH3 is desorbed from the GO.  The relationship between wavelength shift and ammonia gas concentration is shown in Figure 11. The linear fitting results indicate a sensitivity of 4.97 pm/ppm with linear fit coefficient of 98.9% can be achieved in the range of 0-151 ppm ammonia gas concentration. The relationship between wavelength shift and ammonia gas concentration is shown in Figure 11. The linear fitting results indicate a sensitivity of 4.97 pm/ppm with linear fi coefficient of 98.9% can be achieved in the range of 0-151 ppm ammonia gas concentra tion. Figure 11. The wavelength shift as a function of ammonia gas concentration from 0 to 151 ppm.
In order to investigate the reproducibility and reversibility of the proposed sensor we repeatedly injected and extracted the ammonia gas with a concentration of 200 ppm into the gas chamber at a temperature of 25 °C. The transmission spectrum was recorded at intervals of 50 s to determine the dynamic behavior of the gas sensor. After the trans mission spectrum reached a stable state, indicated when the wavelength of the interfer ometer minimum was no longer shifting, the next fill or discharge of ammonia gas took place. We recorded the wavelength shift of the resonant dip during two periods of ammo nia gas fill and discharge, as shown in Figure 12. The results indicate that the dynami response rising time (tr) and falling time (tf) of the sensor are about 5 and 7.5 min for th two periods, respectively. These repeated cycles of exposure to 0 and 200 ppm of NH3 ga demonstrate that the rising and falling time are not very fast, but the sensor exhibits good reversibility. The response and recovery time of the sensor are determined by the thick ness of the GO layer, the diameter of the tapered fiber and the rate of ammonia gas mixing in the chamber on injection or expulsion. Humidity is a common environmental variable which can also cause transmission spectrum changes, so we have tested the humidity response characteristics of the sensor The sensor was put into a constant temperature and humidity chamber (J-TOPH-22-B In order to investigate the reproducibility and reversibility of the proposed sensor, we repeatedly injected and extracted the ammonia gas with a concentration of 200 ppm into the gas chamber at a temperature of 25 • C. The transmission spectrum was recorded at intervals of 50 s to determine the dynamic behavior of the gas sensor. After the transmission spectrum reached a stable state, indicated when the wavelength of the interferometer minimum was no longer shifting, the next fill or discharge of ammonia gas took place. We recorded the wavelength shift of the resonant dip during two periods of ammonia gas fill and discharge, as shown in Figure 12. The results indicate that the dynamic response rising time (t r ) and falling time (t f ) of the sensor are about 5 and 7.5 min for the two periods, respectively. These repeated cycles of exposure to 0 and 200 ppm of NH 3 gas demonstrate that the rising and falling time are not very fast, but the sensor exhibits good reversibility. The response and recovery time of the sensor are determined by the thickness of the GO layer, the diameter of the tapered fiber and the rate of ammonia gas mixing in the chamber on injection or expulsion. The relationship between wavelength shift and ammonia gas concentration is show in Figure 11. The linear fitting results indicate a sensitivity of 4.97 pm/ppm with linear f coefficient of 98.9% can be achieved in the range of 0-151 ppm ammonia gas concentra tion. Figure 11. The wavelength shift as a function of ammonia gas concentration from 0 to 151 ppm.
In order to investigate the reproducibility and reversibility of the proposed sensor we repeatedly injected and extracted the ammonia gas with a concentration of 200 ppm into the gas chamber at a temperature of 25 °C. The transmission spectrum was recorde at intervals of 50 s to determine the dynamic behavior of the gas sensor. After the trans mission spectrum reached a stable state, indicated when the wavelength of the interfer ometer minimum was no longer shifting, the next fill or discharge of ammonia gas too place. We recorded the wavelength shift of the resonant dip during two periods of ammo nia gas fill and discharge, as shown in Figure 12. The results indicate that the dynami response rising time (tr) and falling time (tf) of the sensor are about 5 and 7.5 min for th two periods, respectively. These repeated cycles of exposure to 0 and 200 ppm of NH3 ga demonstrate that the rising and falling time are not very fast, but the sensor exhibits goo reversibility. The response and recovery time of the sensor are determined by the thick ness of the GO layer, the diameter of the tapered fiber and the rate of ammonia gas mixin in the chamber on injection or expulsion. Humidity is a common environmental variable which can also cause transmissio spectrum changes, so we have tested the humidity response characteristics of the sensor The sensor was put into a constant temperature and humidity chamber (J-TOPH-22-B Humidity is a common environmental variable which can also cause transmission spectrum changes, so we have tested the humidity response characteristics of the sensor. The sensor was put into a constant temperature and humidity chamber (J-TOPH-22-B, Jiexin Testing Equipment Co., Ltd., Dongguan, China) with a relative humidity range from 35% to 70%, which basically covers the range of common relative humidity changes. The output transmission spectrum at different relative humidity with the ammonia gas concentration of 0 ppm is shown in Figure 13a. We can see that the wavelength and intensity of the resonant dip will vary when the relative humidity increases from 35% to 70%. GO films are highly hygroscopic due to the presence of oxygen-containing groups on the surface and edges of GO [18,34]. When the relative humidity of the environment increases, the GO film absorbs more water molecules. The water molecules will adhere to the surface of the GO or fill the GO layer, which causes the GO film to swell [25]. Thus, the refractive index of the GO film will decrease as the water molecules increase, and the refractive index difference between the GO film and the fiber cladding will be smaller. When light in the fiber passes through the cladding and reaches the interface between the cladding and the GO film, part of the light is reflected at the interface, and evanescent wave leakage is suppressed and reduced. As a result, the contrast ratio of the transmission spectrum decreases as the relative humidity increases.
Jiexin Testing Equipment Co., Ltd., Dongguan, China) with a relative humidity range from 35% to 70%, which basically covers the range of common relative humidity changes. The output transmission spectrum at different relative humidity with the ammonia gas concentration of 0 ppm is shown in Figure 13a. We can see that the wavelength and intensity of the resonant dip will vary when the relative humidity increases from 35% to 70%. GO films are highly hygroscopic due to the presence of oxygen-containing groups on the surface and edges of GO [18,34]. When the relative humidity of the environment increases, the GO film absorbs more water molecules. The water molecules will adhere to the surface of the GO or fill the GO layer, which causes the GO film to swell [25]. Thus, the refractive index of the GO film will decrease as the water molecules increase, and the refractive index difference between the GO film and the fiber cladding will be smaller. When light in the fiber passes through the cladding and reaches the interface between the cladding and the GO film, part of the light is reflected at the interface, and evanescent wave leakage is suppressed and reduced. As a result, the contrast ratio of the transmission spectrum decreases as the relative humidity increases.
We select the Dip A and B to observe the wavelength variation, shown as in Figure  13b. The wavelength fluctuation of 0.23 and 0.14 nm for Dip A and B is not obvious. Furthermore, the relative humidity in the gas response experiments varies hardly. So, we think that the response of the sensor for ammonia gas is obvious. Figure 13. (a) Transmission spectrum of the sensor at the relative humidity from 35% to 70%. (b) Wavelength fluctuation of dip A and B at the relative humidity from 35% to 70%.
The performances of the fiber sensors for ammonia gas measurement is listed in Table 1. It can be seen that that the proposed sensor has a high sensitivity in a wide range of RH.

Conclusions
This paper proposes a GO-coated Mach-Zehnder fiber interferometer for measurement of ammonia gas concentration. When the GO film absorbs ammonia gas, the change in the refractive index of the film affects the effective refractive index of the cladding mode and the phase of light in the fiber. As a result, the wavelength of the dip in the interfer- Figure 13. (a) Transmission spectrum of the sensor at the relative humidity from 35% to 70%. (b) Wavelength fluctuation of dip A and B at the relative humidity from 35% to 70%.
We select the Dip A and B to observe the wavelength variation, shown as in Figure 13b. The wavelength fluctuation of 0.23 and 0.14 nm for Dip A and B is not obvious. Furthermore, the relative humidity in the gas response experiments varies hardly. So, we think that the response of the sensor for ammonia gas is obvious.
The performances of the fiber sensors for ammonia gas measurement is listed in Table 1. It can be seen that that the proposed sensor has a high sensitivity in a wide range of RH.

Conclusions
This paper proposes a GO-coated Mach-Zehnder fiber interferometer for measurement of ammonia gas concentration. When the GO film absorbs ammonia gas, the change in the refractive index of the film affects the effective refractive index of the cladding mode and the phase of light in the fiber. As a result, the wavelength of the dip in the interferometer response is shifted. The results show that a sensitivity of 4.97 pm/ppm with the linear fit coefficient (R 2 ) of 98.9% can be achieved in the ammonia gas concentration range of 0-151 ppm. The rising time and falling time of the sensor are about 5 and 7.5 min, respectively. The sensitivity can be improved with a thick GO film, but the response time will also increase simultaneously. It has good linear response and stable room temperature recovery. The proposed sensor has the advantages of simple manufacture, small volume, passive design and can be used for on-line monitoring of ammonia gas in a room temperature atmosphere.