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Sensors 2019, 19(2), 228; https://doi.org/10.3390/s19020228

A Fluorescence Inner-Filter Effect Based Sensing Platform for Turn-On Detection of Glutathione in Human Serum

Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, China
These authors contributed equally to this work.
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Received: 23 November 2018 / Revised: 27 December 2018 / Accepted: 2 January 2019 / Published: 9 January 2019
(This article belongs to the Section Biosensors)
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Abstract

A novel turn-on fluorescence assay was developed for the rapid detection of glutathione (GSH) based on the inner-filter effect (IFE) and redox reaction. Molybdenum disulfide quantum dots (MoS2 QDs), which have stable fluorescent properties, were synthesized with hydrothermal method. Manganese dioxide nanosheets (MnO2 NSs) were prepared by exfoliating the bulk δ-MnO2 material in bovine serum albumin (BSA) aqueous solution. The morphology structures of the prepared nanoparticles were characterized by transmission electron microscope (TEM). Studies have shown that the fluorescence of MoS2 QDs could be quenched in the presence of MnO2 NSs as a result of the IFE, and is recovered after the addition of GSH to dissolve the MnO2 NSs. The fluorescence intensity showed a good linear relationship with the GSH concentration in the range 20–2500 μM, the limit of detection was 1.0 μM. The detection method was applied to the analysis of GSH in human serum samples. This simple, rapid, and cost-effective method has great potential in analyzing GSH and in disease diagnosis. View Full-Text
Keywords: fluorescence inner-filter effect; manganese dioxide nanosheets; molybdenum disulfide quantum dots; glutathione detection; human serum fluorescence inner-filter effect; manganese dioxide nanosheets; molybdenum disulfide quantum dots; glutathione detection; human serum
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Tang, S.; You, X.; Fang, Q.; Li, X.; Li, G.; Chen, J.; Chen, W. A Fluorescence Inner-Filter Effect Based Sensing Platform for Turn-On Detection of Glutathione in Human Serum. Sensors 2019, 19, 228.

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