Bismuth Infusion of ABS Enables Additive Manufacturing of Complex Radiological Phantoms and Shielding Equipment

Radiopacity is a critical property of materials that are used for a range of radiological applications, including the development of phantom devices that emulate the radiodensity of native tissues and the production of protective equipment for personnel handling radioactive materials. Three-dimensional (3D) printing is a fabrication platform that is well suited to creating complex anatomical replicas or custom labware to accomplish these radiological purposes. We created and tested multiple ABS (Acrylonitrile butadiene styrene) filaments infused with varied concentrations of bismuth (1.2–2.7 g/cm3), a radiopaque metal that is compatible with plastic infusion, to address the poor gamma radiation attenuation of many mainstream 3D printing materials. X-ray computed tomography (CT) experiments of these filaments indicated that a density of 1.2 g/cm3 of bismuth-infused ABS emulates bone radiopacity during X-ray CT imaging on preclinical and clinical scanners. ABS-bismuth filaments along with ABS were 3D printed to create an embedded human nasocranial anatomical phantom that mimicked radiological properties of native bone and soft tissue. Increasing the bismuth content in the filaments to 2.7 g/cm3 created a stable material that could attenuate 50% of 99mTechnetium gamma emission when printed with a 2.0 mm wall thickness. A shielded test tube rack was printed to attenuate source radiation as a protective measure for lab personnel. We demonstrated the utility of novel filaments to serve multiple radiological purposes, including the creation of anthropomorphic phantoms and safety labware, by tuning the level of radiation attenuation through material customization.


Introduction
The field of medical imaging spans the preclinical and clinical research space with several imaging modalities including magnetic resonance imaging (MRI), ultrasound (US), and three-dimensional patent-pending ABS-bismuth composite filaments, commercialized under the name "GMASS," were designed for 3D printing high-density components with adjustable radiopaque properties. By tuning the bismuth concentration, filaments with a radiopacity similar to bone were fabricated and used to 3D print a multi-material nasocranial phantom derived from patient CT scan data. An increased bismuth concentration was employed to 3D print a sample tube rack with improved radiation attenuation properties, demonstrating the customization of protective personnel safety equipment. These filaments had radiopacity properties that were not were not available from an array of commercial 3D printing filaments measured.

Preparation of ABS-Bismuth Composites of Varied Concentration
The filaments for this study were manufactured by Turner MedTech (Orem, UT, USA). The standard ABS-bismuth GMASS product has a density of 2.7 g/cm 3 . However, for the purposes of this study several filaments were manufactured that varied in density over the range of 2.5 g/cm 3 to 1.2 g/cm 3 . To make the various concentrations, GMASS filaments were reground into pellets that were equally divided by weight into containers in which base ABS resin was added to achieve the desired filament density. Each container of pellets was well mixed and then sequentially processed through a screw extruder starting from the least desired density to the greatest. The extruded filament was then divided into sections and density samples were taken to characterize the filament and ensure that each met the requirements of this study.

Preclinical CT Acquisition and Reconstruction of Filaments
CT scan images of the individual filaments were acquired using an Albira CT system (Bruker, Billerica, MA, USA). The X-ray source settings were a 400 µA current at a 45 kV voltage with a 0.5 mm aluminum filter in the X-ray path for hardening. Circular scans of the filaments were taken with 400 projections per scan and a field of view (FOV) of 64 mm, and reconstructed with a 250 µm isotropic voxel size using the Albira Suite 5.0 Reconstructor, with "Filtered Back Projection" (FBP) algorithm calibrated to output data in Hounsfield Units (HU). Following the scan, data was opened in its native microPET file format and opened with PMOD software for further analysis.

Clinical CT Acquisition of Filaments
Images of the filaments were acquired using a clinical NeuroLogica CereTom CT scanner (NeuroLogica, Danvers, MA, USA). Data was collected using an axial test protocol with 100 kV source and current of 3 mA, with imaging resolution timing of 6 s. For each scan, the coverage length was 230 mm, with 184 images taken over a 138 s scan time to yield a reconstructed data set with voxel size of 0.5 mm × 0.5 mm × 0.625 mm and calibrated to HU. Following the scan, data was exported as a DICOM format file and imported to PMOD software for further analysis.

Radiodensity Measurement of CT Image Data
Pre-clinical or clinical CT data was opened in PMOD software (V 3.2, PMOD Technologies LLC, Zurich, Switzerland) for Volume of Interest (VOI) measurement of average HU (with standard deviation of voxel density) from each filament. Data were recorded and plotted in Graphpad Prism (Graphpad Software Inc., La Jolla, CA, USA).

Multimaterial Phantom
A de-identified, clincial CT imaging data set of a patient skull and sinuses (with signed patient auhorization for release) was acquired and reconstructed with a 0.4 mm isotropic voxel via a MiniCAT (Xoran Technologies, Ann Arbor, MI, USA) CT and associated software. Image files were imported into PMOD 3.2, and the volume to be printed containing the head was cropped by creating a volume of interest and masking the image outside the defined volume. The resulting image file was saved in nifti format and imported into 3D Slicer [24]. A threshold of 180 was applied to isolate bone tissue and a second threshold of −200 was applied to generate surface maps using the greyscale modelmaker tool. The surface maps were then exported as STL files. These files were opened in NetFabb (Autodesk, San Rafael, CA, USA) and extraneous portions of the images and degenerate faces were removed. Fine portion removal was done in MeshLab (ISTI-CNR). The surface maps were then exported as STL files to be uploaded for printing. The STL files were then imported independently into MakerBot Desktop's (MakerBot Industries, New York, NY, USA) simulated build platform. Once in MakerBot Desktop the models were carefully aligned with one another to accurately reflect the full CT data. The 3D printer used for producing this particular phantom was the MakerBot Replicator 2X (MakerBot Industries) with a 0.4 mm nozzle diameter, 11 µm XY plane precision, 2.5 µm Z position precision, and a printing layer height of 0.2 mm. The soft tissue model was printed with native ABS using two perimeter shells and 15% hexagonal infill, while the bone model was printed using 1.2 g/cm 3 ABS-bismuth composite with four perimeter shells and 15% infill. The models were printed simultaneously on a heated build plate using duel extruders without support structures to provide an accurate mimic of the CT scan.

Squares and Example Personnel Protective Equipment
A 16-position test tube rack was printed solid using the 2.7 g/cm 3 ABS-bismuth composite on the MakerBot Replicator 2X as noted above. Attenuation squares of 5 cm × 5 cm were created of varied thickness (1, 2, and 3 mm) using Autodesk Inventor, and printed on the MakerBot Replicator 2X in both native ABS and 2.7 g/cm 3 ABS-bismuth composite.

3D-Printed Model Accuracy Measurements
CT scans of the 3D-printed multimaterial head phantom were acquired using a clinical NeuroLogica CereTom CT scanner using the same acquisition parameters as used for measuring the ABS-bismuth filaments. The radiodensity was measured at five distinct regions of the phantom, all in areas corresponding to bone tissue. The radiodensity of the mimetic bone in these regions was compared to the measured radiodensity at the same locations in the original patient CT scan.
The head phantom was measured using a digital caliper at eight points, encompassing both the soft and bone tissue portions. These measurements were compared with distance measurements taken on the digital DICOM file of the patient CT scan data. The absolute percent difference in measurements was recorded at each of the eight measurement points.

Radiation Attenuation Measurements
Radiation attenuation experiments for comparison of 2.7 g/cm 3 ABS-bismuth relative to native ABS were conducted using 99m Technetium ( 99m Tc) pertechnetate sourced from a local Cardinal Health (South Bend, IN, USA) nuclear pharmacy. Sources were diluted to 390, 180, 87, and 41 µCi activities in 100 µL of solution, each of which was measured immediately prior to experimentation using a dose calibrator (AtomLab 400, Biodex Medical Systems Inc., Shirley, NY, USA). Gamma emission from each source was captured using an Xtreme 4MP image station (Bruker Preclinical Imaging, Billerica, MA, USA) fitted with a radioisotopic phosphor screen and sensitive CCD chip cooled to −65 • C. Images were acquired using a 100 mm FOV, binning 4 × 4, and 10 s exposure time. Each point source was imaged thirteen times: without any block, and then at 1, 2, 3, 4, 5, and 6 mm of thickness of each attenuation square (or stack of squares) comprised of either native ABS or 2.7 g/cm 3 ABS-bismuth composite. Region of interest analysis of each image was conducted using ImageJ software, and mean intensity values recorded in Microsoft Excel. Ratios were calculated at each thickness using ABS-bismuth values as the numerator and native ABS the denominator. Normalized ratios were then averaged with SEM (n = 4), plotted using GraphPad Prism and fitted to a single exponential decay to output half-life values as a measure of width of ABS-bismuth composite needed to block 50% of radiation from 99 m Tc.
Radiation attenuation experiments for comparison of the 2.7 g/cm 3 ABS-bismuth printed tube rack (shielded) relative to a standard tube rack (traditional) were conducted using 99m Tc solutions in standard 1.5 mL microfuge tubes. Sources were diluted to 224, 445, and 950 µCi activities in 500 µL of solution and measured immediately prior to experimentation using the same dose calibrator. Single tubes were placed in the front row of both the shielded and traditional tube racks and the transmitted gamma radiation was measured with a Geiger counter (Ludlum Measurements Inc., Sweetwater, TX, USA) placed 12 in from the edge of the tube rack. Tubes were then moved to the second row of their respective racks and measured again.

Three Dimensional (3D) Printing Filaments to Mimic Human Tissues
The radiodensities of 11 different 3D-printed filaments were measured by taking CT scans of the filaments and quantifying the images using the Hounsfield Unit (HU) scale (Table 1). This scale is benchmarked with air at −1000 HU and water at 0 HU. Soft tissue has a radiopacity range of −100-100 HU [25,26] while bone has values of HU that typically measure 1000-3000 HU [27]. The chosen filaments ranged from polymers, such as ABS (natural and glow), ABS derivatives (Bendlay, IGUS iglidur), and PLA (polylactic acid, natural and glow), to polymer composites, such as Laybrick and woodFill, to metal-polymer composites, such as copperFill, bronzeFill, and GMASS bismuth (GMASS). The filaments had a wide range of radiodensities, with the polymers having lower values and the metallic composite filaments having higher ones. Based on the results, ABS was chosen to represent soft tissue since it had a radiodensity in the middle of the expected range. However, none of the measured filaments matched the expected radiodensity of bone of 1000-3000 HU.
In order to fabricate a filament that more closely emulated bone, a range of bismuth-infused ABS filaments was created by diluting GMASS in increasing amounts of ABS. The radiodensity of these filaments was measured on both a preclinical CT and a clinical CT system, along with standard ABS and GMASS filaments (Table 2, Figure 1).   As expected, the radiodensity increased with the increasing concentration of bismuth. ABS-bismuth 1, with a density of 1.20 g/cm 3 , was the only filament with a radiopacity in the range of 1000-3000 HU and was therefore the best candidate for bone in phantom models. A multi-material nasocranial phantom was 3D printed, where soft tissue was printed in ABS (pink color) and bone was printed in ABS-bismuth 1 (Figure 2a). A clinical CT scan of the nasocranial phantom showed the bone portion appearing at a higher contrast (whiter color on grayscale image), demonstrating the higher radiopacity of the ABS-bismuth 1 portion (coronal slice shown in Figure 2b). The CT scan image was then processed into a 3D digital rendering, clearly showing the bone portion of the nasocranial phantom (light) and its stark contrast with the soft tissue section (gray) (Figure 2c). The radiopacity of the bone tissue in the phantom was compared to the original patient CT scan data at five distinct, corresponding regions. All measurements on both datasets fell within the expected 1000-3000 HU range for bone (Figure 2d). The dimensional accuracy of the phantom was determined by measuring the printed model and comparing it to measurements from the original CT scan data. Overall, the average percent difference in dimensions between the model and the CT data was 1.71%, with a maximum difference of 4.00% (Figure 2e). As expected, the radiodensity increased with the increasing concentration of bismuth. ABS-bismuth 1, with a density of 1.20 g/cm 3 , was the only filament with a radiopacity in the range of 1000-3000 HU and was therefore the best candidate for bone in phantom models. A multi-material nasocranial phantom was 3D printed, where soft tissue was printed in ABS (pink color) and bone was printed in ABS-bismuth 1 (Figure 2a). A clinical CT scan of the nasocranial phantom showed the bone portion appearing at a higher contrast (whiter color on grayscale image), demonstrating the higher radiopacity of the ABS-bismuth 1 portion (coronal slice shown in Figure 2b). The CT scan image was then processed into a 3D digital rendering, clearly showing the bone portion of the nasocranial phantom (light) and its stark contrast with the soft tissue section (gray) (Figure 2c). The radiopacity of the bone tissue in the phantom was compared to the original patient CT scan data at five distinct, corresponding regions. All measurements on both datasets fell within the expected 1000-3000 HU range for bone (Figure 2d). The dimensional accuracy of the phantom was determined by measuring the printed model and comparing it to measurements from the original CT scan data. Overall, the average percent difference in dimensions between the model and the CT data was 1.71%, with a maximum difference of 4.00% (Figure 2e). (d) radiodensity data from original patient CT scan and from 3D-printed phantom CT scan at five coordinated bone locations; (e) distance measurements taken from the original patient CT scan data and from the physical 3D-printed phantom at eight coordinated locations, along with the absolute value of the percent change in distance between the two measures.

Three Dimensional (3D) Printing Filaments for Radiation Attenuation
While the radiopacity of filaments was titrated down by incorporating ABS, radiodense materials can attenuate high levels of radioactivity. Therefore, the GMASS filament was selected to quantify its ability to shield radiation. This was tested using different levels of a radioactive 99m Technetium ( 99m Tc) gamma emission source isotope and 3D-printed, GMASS squares of 1, 2, and 3 mm thickness ( Figure  3a). The mean normalized radiation intensity decreased in an exponential fashion with the increasing GMASS sheet thickness, independent of the starting activity (Figure 3b). The four curves were averaged and fit to a one-phase exponential decay (R 2 = 0.99), yielding a half-life value of 2.0 mm, indicating that this thickness of GMASS plastic will attenuate 50% of the 99m Tc radiation. A test tube rack was 3D printed from the GMASS filament, with a minimum wall thickness of 2 mm along the outer edge, to demonstrate the ability to print a customized, three-dimensional object capable of shielding a minimum of 50% of radiation from the 99m Tc isotope (Figure 3c). The ability of the shielded tube rack to attenuate radiation was compared to a traditional tube rack by placing 99m Tc radiation sources in multiple positions in each rack and measuring the transmitted radiation. The shielded rack transmitted between 60% and 85% less gamma radiation than the traditional rack, depending on the initial source radioactivity and the tube placement in either the first or second row of the respective racks (Figure 3d). The shielded rack attenuated a greater percentage of radiation as the source intensity decreased. Additionally, placing the source in the second row, thereby increasing the amount of GMASS between the source and the detector, reduced the transmitted radiation by an additional 60%-70% relative to source placement in the first row.

Three Dimensional (3D) Printing Filaments for Radiation Attenuation
While the radiopacity of filaments was titrated down by incorporating ABS, radiodense materials can attenuate high levels of radioactivity. Therefore, the GMASS filament was selected to quantify its ability to shield radiation. This was tested using different levels of a radioactive 99m Technetium ( 99m Tc) gamma emission source isotope and 3D-printed, GMASS squares of 1, 2, and 3 mm thickness (Figure 3a). The mean normalized radiation intensity decreased in an exponential fashion with the increasing GMASS sheet thickness, independent of the starting activity (Figure 3b). The four curves were averaged and fit to a one-phase exponential decay (R 2 = 0.99), yielding a half-life value of 2.0 mm, indicating that this thickness of GMASS plastic will attenuate 50% of the 99m Tc radiation. A test tube rack was 3D printed from the GMASS filament, with a minimum wall thickness of 2 mm along the outer edge, to demonstrate the ability to print a customized, three-dimensional object capable of shielding a minimum of 50% of radiation from the 99m Tc isotope (Figure 3c). The ability of the shielded tube rack to attenuate radiation was compared to a traditional tube rack by placing 99m Tc radiation sources in multiple positions in each rack and measuring the transmitted radiation. The shielded rack transmitted between 60% and 85% less gamma radiation than the traditional rack, depending on the initial source radioactivity and the tube placement in either the first or second row of the respective racks (Figure 3d). The shielded rack attenuated a greater percentage of radiation as the source intensity decreased. Additionally, placing the source in the second row, thereby increasing the amount of GMASS between the source and the detector, reduced the transmitted radiation by an additional 60%-70% relative to source placement in the first row. . 3D-printed GMASS attenuation of radiation from 99m Tc emission sources: (a) 3D-printed sheets made from ABS and GMASS filaments with thickness ranging from 1 to 3 mm that were stacked to make sheets with thickness between 1 and 6 mm; (b) Plot of mean normalized radiation intensity transmitted through GMASS sheets of varying thickness, normalized to ABS sheets of the same thickness. Each sheet thickness was subject to multiple radiation sources with distinct intensities, and an average normalized radiation intensity was calculated (black line); (c) 3D-printed test tube rack made from GMASS filament; Plot of transmitted radiation through the shielded GMASS tube rack and a traditional tube rack from 99m Tc emission sources with varying initial radioactivity levels.

Discussion
With the wide range of materials currently available for 3D printing, many choices are available when constructing a phantom for CT imaging. However, in order to create a truly anthropomorphic device, it is necessary to select materials which mimic the imaging properties, such as X-ray opacity, of native tissues. However, there is not a thorough repository of radiation property data for 3D printing materials [23]. Furthermore, since various imaging techniques utilize different imaging energy levels, the materials must have the proper radiopacity at multiple incident energy levels. For instance, clinical CT systems use a higher energy imaging source than preclinical CT systems due to the need to penetrate larger human anatomy. Indeed, the clinical CT system showed a highly linear relationship between radiopacity and filament density using a 100 kVp source, as this energy level was able to penetrate the filaments with higher bismuth concentrations. The lower-energy 45 kVp preclinical system showed a linear relationship up to 1.3 g/cm 3 of bismuth, followed by a plateau as the radiation source became almost completely attenuated. The ABS-bismuth 1 filament yielded HU values of 2113.0 and 2603.4 on the preclinical and clinical systems, respectively, making it the only filament tested that had imaging properties in the expected 1000-3000 HU range to emulate bone on both instruments. When creating a mimetic phantom it is important to note that native bone will not have a single value of radiopacity as it is heterogeneous tissue with varying densities.
The ABS-bismuth 1 filament was used in the fabrication of a 3D-printed, multi-material nasocranial phantom. The CT images and three-dimensional rendering of the CT scan both clearly showed the bone structure of the phantom, while the radiodensity measurements showed the strong correlation between the phantom and the original patient CT data, demonstrating that ABS-bismuth 1 is an appropriate imaging mimic for bone tissue. The dimensions of the printed model matched the original patient scan data with an average accuracy greater than 98%. This level of accuracy was expected because the resolution of the 3D printer is better than that of the original CT scan dataset. Because the ABS-bismuth 1 filament can be incorporated on consumer-grade 3D printers in facile fashion, it provides a promising option for creating accurate, patient-specific models. For example, the anthropomorphic nasocranial phantom could be used as a teaching aid for a CT technician to . 3D-printed GMASS attenuation of radiation from 99m Tc emission sources: (a) 3D-printed sheets made from ABS and GMASS filaments with thickness ranging from 1 to 3 mm that were stacked to make sheets with thickness between 1 and 6 mm; (b) Plot of mean normalized radiation intensity transmitted through GMASS sheets of varying thickness, normalized to ABS sheets of the same thickness. Each sheet thickness was subject to multiple radiation sources with distinct intensities, and an average normalized radiation intensity was calculated (black line); (c) 3D-printed test tube rack made from GMASS filament; Plot of transmitted radiation through the shielded GMASS tube rack and a traditional tube rack from 99m Tc emission sources with varying initial radioactivity levels.

Discussion
With the wide range of materials currently available for 3D printing, many choices are available when constructing a phantom for CT imaging. However, in order to create a truly anthropomorphic device, it is necessary to select materials which mimic the imaging properties, such as X-ray opacity, of native tissues. However, there is not a thorough repository of radiation property data for 3D printing materials [23]. Furthermore, since various imaging techniques utilize different imaging energy levels, the materials must have the proper radiopacity at multiple incident energy levels. For instance, clinical CT systems use a higher energy imaging source than preclinical CT systems due to the need to penetrate larger human anatomy. Indeed, the clinical CT system showed a highly linear relationship between radiopacity and filament density using a 100 kVp source, as this energy level was able to penetrate the filaments with higher bismuth concentrations. The lower-energy 45 kVp preclinical system showed a linear relationship up to 1.3 g/cm 3 of bismuth, followed by a plateau as the radiation source became almost completely attenuated. The ABS-bismuth 1 filament yielded HU values of 2113.0 and 2603.4 on the preclinical and clinical systems, respectively, making it the only filament tested that had imaging properties in the expected 1000-3000 HU range to emulate bone on both instruments. When creating a mimetic phantom it is important to note that native bone will not have a single value of radiopacity as it is heterogeneous tissue with varying densities.
The ABS-bismuth 1 filament was used in the fabrication of a 3D-printed, multi-material nasocranial phantom. The CT images and three-dimensional rendering of the CT scan both clearly showed the bone structure of the phantom, while the radiodensity measurements showed the strong correlation between the phantom and the original patient CT data, demonstrating that ABS-bismuth 1 is an appropriate imaging mimic for bone tissue. The dimensions of the printed model matched the original patient scan data with an average accuracy greater than 98%. This level of accuracy was expected because the resolution of the 3D printer is better than that of the original CT scan dataset. Because the ABS-bismuth 1 filament can be incorporated on consumer-grade 3D printers in facile fashion, it provides a promising option for creating accurate, patient-specific models. For example, the anthropomorphic nasocranial phantom could be used as a teaching aid for a CT technician to visualize the complex nasal structure due to its fidelity relative to the original patient data. Leary et al. noted the ability of high-accuracy phantoms to serve as better teaching models, thereby improving treatment outcomes [23]. Additionally, the tunability of the radiopacity makes it possible to use these ABS-bismuth filaments for 3D printing implantable materials, such as pins, plates, or screws, which would be straightforward to visualize during CT imaging. Additional research on other material properties, such as modulus, would be necessary to better understand the potential applications of ABS-bismuth 1.
In cases where the goal is to maximize radiation attenuation, as opposed to matching a radiopacity of a known tissue, increasing the bismuth concentration and overall density of the filament was found to be effective. As expected, the undiluted GMASS bismuth (2.7 g/cm 3 ) filament had the highest radiopacity, meaning it was the optimal filament tested to shield against radiation transmission. Radiation shielding is important in labware, personal protective equipment, and equipment design to safeguard personnel from excessive radiation exposure. Therefore, we compared the ability of GMASS sheets to shield radiation from a common isotope used in biomedical single photon emission computed tomography (SPECT) imaging: 99m Tc with a gamma emission energy of 140 keV. Attenuation measurements were made and normalized to standard ABS to quantify the thickness of GMASS needed to block 50% of the 99m Tc radiation. The data in Figure 3 suggest that 2.0 mm of GMASS filament will attenuate half of the emission from this isotope relative to 2.0 mm of standard ABS. This makes it a strong candidate for radiation shielding of isotopes in this energy range or lower, especially for applications where easily printable and thin materials are required. A test tube rack that can be used to hold radioactive samples was 3D printed as a demonstration, but additional applications such as thin shielding for protective clothing or radiation source containment in imaging systems are also possible. It could also have clinical applications as 3D printing has been used to create customized radiation shields for a patient undergoing radiotherapy to protect surrounding healthy tissue [28,29].
Future work may entail further customization of the base GMASS filament to incorporate luminescent dyes or light-scattering nanoparticles to allow 3D-printed structures to be visible on a wider array of imaging devices, such as during optical fluorescence or luminescence acquisitions. Nanoprobes for multimodal biological imaging, including fluorescence, CT, and MR, have been synthesized and could serve as a starting point for multifunctional imaging dopants [30]. Thermoluminescent nanoparticles have been developed for use as radiotherapy dosimeters [31]. A GMASS filament doped with these particles could be used to train personnel on the CT equipment while simultaneously measuring the radiotherapy dose delivered to the phantom. The ability to customize the imaging properties of filaments shows promise for the future creation of myriad devices, objects, and implants that can be mass produced and finely tailored to suit specific needs within the medical space in a cost-effective manner.

Conclusions
The applications of 3D printing have been rapidly expanding as the underlying technology has matured, costs have decreased, and accessibility has increased. Three-dimensional printing for medical applications, particularly for medical imaging phantoms for training or calibration, has become an area of significant interest. To date, little research has focused on selecting printing materials that closely mimic the imaging properties of the native tissues they are being selected to represent. In this work, the initial focus was on characterizing one particular imaging property, the radiopacity, of different common 3D printing filaments as potential mimics for bone and soft tissue in a CT scan. ABS, a commonly utilized polymer, was found to be an appropriate soft tissue replica. In order to mimic bone, a commercially available bismuth-infused filament was diluted with ABS, demonstrating the ability to tune filament radiodensity and fabricate a bone mimetic material under CT. By combining ABS and bismuth-infused ABS, a nasocranial phantom was produced with the desired CT imaging properties.
Additionally, maximizing the bismuth infusion created a filament that attenuated radiation to build objects such as protective labware or apparel. The ability to tune the radiopacity of a mainstream, printable filament opens up many future applications for uses within the medical imaging and lab safety spaces.