Synthesis and Cholinesterase Inhibitory Potency of 2,3-Indolo-oleanolic Acid and Some Related Derivatives

: In this study, the synthesis and biological activities of previously and newly synthesized oleanolic acid derivatives containing seven-membered cyclic amines at the C28 position were described. The obtained compounds were fully characterized via 1 H and 13 C NMR spectroscopy, and the bioactivity was evaluated by Ellman’s method. Among the tested compounds, 2,3-indolo-oleanolic acid was found to be the most active compound with an IC 50 value of 0.78 µ M against acetylcholinesterase. These results are signiﬁcant due to the fact that research on the inhibition of acetylcholinesterase and butyrylcholinesterase enzymes by oleanolic acid, in particular indoloderiva-tives, is limited.


Introduction
Progressive and irreversible neurodegenerative diseases, such as Alzheimer's disease (AD), characterized by memory loss, behavioral, and many other cognitive impairments, are socially significant diseases that require active attention from scientists.Currently available therapies for AD primarily focus on relieving its symptoms by targeting the cholinergic system [1].Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are enzymes involved in the breakdown of the neurotransmitter acetylcholine in cholinergic synapses.AChE and BChE remain the most promising and therapeutic targets for symptomatic AD treatment.The "cholinergic hypothesis" [2] underlies the rationale behind using AChE and BChE inhibitors.Inhibiting cholinesterase activity can compensate for the cholinergic deficiency, leading to improvements in learning and memory [3].In recent years, the development of drugs for AD has been dominated by research into multitargeted treatments that can slow down the progression of the disease.Therefore, new strategies for the search and synthesis of cholinesterase inhibitors remain relevant.
Natural products, in particular triterpenoids, are actively used in organic and medicinal chemistry.At present, the obtained semi-synthetic analogs are considered new classes of antitumor, antiviral, antimicrobial, and other agents [4][5][6][7].
One of the most promising candidates for future clinical trials includes triterpenoids conjugated or annulated with heterocyclic moieties [8].Thus, indolo-derivatives, along with different biological activities such as anticancer activity [9], exhibited good enzyme inhibition properties.For example, 2,3-indolo-betilinic acid was found to be the most active inhibitor of α-glucosidase, with an IC 50 of 1.8 µM, being 221-fold more active than the market drug acarbose [10].Further modification by amidation with glycine and L-phenylalanine at C28 led to derivatives with IC 50 values of 0.04 and 0.05 µM, being 3784-and 4730-fold more active than acarbose [11].2,3-Indolo-oleanolic with cyclohexane Molbank 2023, 2023, M1739 2 of 7 amide at C28 was found to be a NO production inhibitor with an IC 50 2.66 µM [12].The indolo-glycyrrhetinic acid with a trifluoromethyl substitute in the indole ring exhibited non-competitive inhibition of PTP1B with an IC 50 2.5 µM [13].
Compared to other types, the inhibition of enzymes of the esterase family by indole acids has not been studied, although the influence of modification of the C28 position by amines has been noted in the literature.For example, a derivative of betulinic acid with a pyrrolidine substituent exhibited inhibitory properties against BChE with Ki values of 0.39 ± 0.04 µM, while the replacement of the pyrrolidine substituent with piperidinyl led to selective inhibition of AChE (Ki 1.00 ± 0.09 µM) [14].The amides of lupane, oleanane, and ursane types of triterpene acids with a fragment of 1,3-and 1,4-diazabicyclononane and their methylated salts turned out to be highly specific inhibitors of BChE with a degree of inhibition above 95% [15].A derivative of glycyrrhetic acid with a triethyldiamine moiety at position C30 exhibited selective butyrylcholinesterase inhibitory activity with a Ki value of 5.43 µM [16].
In line with these data, the main goal of this research was the study of the acetylcholine inhibitory activity of oleanolic acid derivatives, including those with a modified C28 position as well as in combination with the A-indole cycle.
Compared to other types, the inhibition of enzymes of the esterase family by indole acids has not been studied, although the influence of modification of the C28 position by amines has been noted in the literature.For example, a derivative of betulinic acid with a pyrrolidine substituent exhibited inhibitory properties against BChE with Ki values of 0.39 ± 0.04 µM, while the replacement of the pyrrolidine substituent with piperidinyl led to selective inhibition of AChE (Ki 1.00 ± 0.09 µM) [14].The amides of lupane, oleanane, and ursane types of triterpene acids with a fragment of 1,3-and 1,4-diazabicyclononane and their methylated salts turned out to be highly specific inhibitors of BChE with a degree of inhibition above 95% [15].A derivative of glycyrrhetic acid with a triethyldiamine moiety at position C30 exhibited selective butyrylcholinesterase inhibitory activity with a Ki value of 5.43 µM [16].
In line with these data, the main goal of this research was the study of the acetylcholine inhibitory activity of oleanolic acid derivatives, including those with a modified C28 position as well as in combination with the A-indole cycle.
Compared to other types, the inhibition of enzymes of the esterase family by indole acids has not been studied, although the influence of modification of the C28 position by amines has been noted in the literature.For example, a derivative of betulinic acid with a pyrrolidine substituent exhibited inhibitory properties against BChE with Ki values of 0.39 ± 0.04 µM, while the replacement of the pyrrolidine substituent with piperidinyl led to selective inhibition of AChE (Ki 1.00 ± 0.09 µM) [14].The amides of lupane, oleanane, and ursane types of triterpene acids with a fragment of 1,3-and 1,4-diazabicyclononane and their methylated salts turned out to be highly specific inhibitors of BChE with a degree of inhibition above 95% [15].A derivative of glycyrrhetic acid with a triethyldiamine moiety at position C30 exhibited selective butyrylcholinesterase inhibitory activity with a Ki value of 5.43 µM [16].
In line with these data, the main goal of this research was the study of the acetylcholine inhibitory activity of oleanolic acid derivatives, including those with a modified C28 position as well as in combination with the A-indole cycle.
The structure of the newly synthesized compounds was confirmed by 1 H and 13 C NMR spectra.Thus, for cyanomethyl derivatives 8 and 12, the signals of the carbon atom of the CN group were observed at δ 115 ppm.In the 1 Н and 13 С NMR spectra of compound 10, the signals of proton and carbon atoms of ethoxy groups were observed in the range δН 4.10-4.17ppm, δС 63.62 and 63.66 ppm.
All compounds were tested for their ability to inhibit the activity of recombinant human acetylcholinesterase and human butyrylcholinesterase from blood plasma in vitro according to the Ellman method [20] on a Shimadzu UV-1800 spectrophotometer by measuring the color intensity change at a wavelength of 412 nm for 2 min at 25 °C.Donepezil was used as a reference drug (Table 1).
Among hopopiperazinamides, moderate activity was observed for 3-oxo-derivative 6 (IC50 17.7 µM), while the introduction of indolo-group into the oleanolic acid molecule led to an increase in the IC50 to 107 µM (compound 11).N-Cyanomethylation of compounds 6 and 11 improved the activity of the 2,3-indolo derivative 12 (IC50 12.8 µM), while for the 3-oxo-homopiperazine amide 7, the IC50 value increased 1.5 times (IC50 27.7 µM).The introduction of a hydroxymethylene group into the polycyclic ring led to moderate inhibition of butyrylcholinesterase with an IC50 value of 38.8 µM for 3-acetoxy derivative 3, which was not detected for other compounds with a cyanomethyl group.The products of the Claisen-Schmidt reaction with a 3-or 4-pyridine group in the C2 position were inactive (compound 5, 156 µM) or moderately active (compound 4, 27.4 µM).
At the same time, 2,3-indolo-oleanolic acid 9 was found to be the most active among all the tested compounds, with an IC50 value of 0.78 µM, albeit to a lesser extent compared to the effects of donepezil (0.01 µM).In general, according to the value of the average inhibitory concentration, the following pattern can be revealed for the modified derivatives: 2,3-indolo < 3-oxo < 3-acetoxy.
It should be noted that 2,3-indolo-oleanolic acid was also evaluated for its ability to inhibit various enzymes.For example, it was shown that 9 exhibit PTP1B inhibition properties with an IC50 of 0.61 µM [21].Additionally, 2,3-indolo-oleanolic acid 9 was found to be a moderate α-glucosidase inhibitor with an IC50 of 5.5 µM and 72-fold more active than the market drug acarbose [10].Considering that studies of indolo-triterpene acid derivatives are limited only to these enzymes, the results obtained in this article are of great interest.The structure of the newly synthesized compounds was confirmed by 1 H and 13 C NMR spectra.Thus, for cyanomethyl derivatives 8 and 12, the signals of the carbon atom of the CN group were observed at δ 115 ppm.In the 1 H and 13 C NMR spectra of compound 10, the signals of proton and carbon atoms of ethoxy groups were observed in the range δ H 4.10-4.17ppm, δ C 63.62 and 63.66 ppm.
All compounds were tested for their ability to inhibit the activity of recombinant human acetylcholinesterase and human butyrylcholinesterase from blood plasma in vitro according to the Ellman method [20] on a Shimadzu UV-1800 spectrophotometer by measuring the color intensity change at a wavelength of 412 nm for 2 min at 25 • C. Donepezil was used as a reference drug (Table 1). 1 inhibitory concentration values are expressed as the mean of three independent measurements; the acetylthiocholine and butyrylthiocholine concentration was 1 mM. 2 determination of inhibitory activity was limited by compound solubility in phosphate buffer.
Among hopopiperazinamides, moderate activity was observed for 3-oxo-derivative 6 (IC 50 17.7 µM), while the introduction of indolo-group into the oleanolic acid molecule led to an increase in the IC 50 to 107 µM (compound 11).N-Cyanomethylation of compounds 6 and 11 improved the activity of the 2,3-indolo derivative 12 (IC 50 12.8 µM), while for the 3-oxo-homopiperazine amide 7, the IC 50 value increased 1.5 times (IC 50 27.7 µM).The introduction of a hydroxymethylene group into the polycyclic ring led to moderate inhibition of butyrylcholinesterase with an IC 50 value of 38.8 µM for 3-acetoxy derivative 3, which was not detected for other compounds with a cyanomethyl group.The products of the Claisen-Schmidt reaction with a 3-or 4-pyridine group in the C2 position were inactive (compound 5, 156 µM) or moderately active (compound 4, 27.4 µM).
At the same time, 2,3-indolo-oleanolic acid 9 was found to be the most active among all the tested compounds, with an IC 50 value of 0.78 µM, albeit to a lesser extent compared to the effects of donepezil (0.01 µM).In general, according to the value of the average inhibitory concentration, the following pattern can be revealed for the modified derivatives: 2,3-indolo < 3-oxo < 3-acetoxy.
It should be noted that 2,3-indolo-oleanolic acid was also evaluated for its ability to inhibit various enzymes.For example, it was shown that 9 exhibit PTP1B inhibition properties with an IC 50 of 0.61 µM [21].Additionally, 2,3-indolo-oleanolic acid 9 was found to be a moderate α-glucosidase inhibitor with an IC 50 of 5.5 µM and 72-fold more active than the market drug acarbose [10].Considering that studies of indolo-triterpene acid derivatives are limited only to these enzymes, the results obtained in this article are of great interest.

Materials and Methods
The spectra were recorded at the Center for the Collective Use 'Chemistry' of the Ufa Institute of Chemistry of the UFRC RAS and RCCU "Agidel" of the UFRC RAS. 1 H and 13 C NMR spectra were recorded on a "Bruker Avance-III" (Bruker, Billerica, MA, USA, 500 and 125.5 MHz, respectively, δ, ppm, Hz) in CDCl 3 .Tetramethylsilane was used as an internal standard.Mass spectra were obtained on a liquid chromatograph-mass spectrometer LCMS-2010 EV (Shimadzu, Kyoto, Japan).Melting points were detected on a microtable "Rapido PHMK05" (Nagema, Dresden, Germany).Optical rotations were measured on a polarimeter Perkin-Elmer 241 MC (PerkinElmer, Waltham, MA, USA) in a tube length of 1 dm.Thin-layer chromatography analyses were performed on Sorbfil plates (Sorbpolimer, Krasnodar, Russia), using the solvent system chloroform-ethyl acetate at a ratio of 40:1.Substances were detected by spraying the plates with a 10% sulfuric acid solution with subsequent heating at 100-120 • C for 2-3 min.All chemicals were of reagent grade (Sigma-Aldrich, St. Louis, MO, USA).Compounds 1 [17], 2 and 3 [18], 4-7 and 11 [19], 9 [22] were synthesized as described previously.All spectral data of new compounds are provided in the Supplementary Material file.

Biological Assay In Vitro Cholinesterase Inhibition Assay
The inhibitory activity of compounds against human AChE and human BChE was measured using the Ellman's method [20].Stock solutions (0.01 M) of compounds were dissolved in H 2 O, or ethanol.In the case of compounds soluble in ethanol, the final concentration of ethanol in the cuvette was 0.1% vol.Enzyme-catalyzed substrate hydrolysis was carried out in 0.1 m phosphate buffer (pH 8.0 for AChE and pH 7.0 for BChE) containing 0.25 U recombinant human AChE or 0.25 U human BChE from blood plasma, 0.1 mM 5,5 -dithiobis-(2-nitrobenzoic acid) and 1 mM acetylthiocholine (ATC) or butyrylthiocholine (BTC) as substrates.All assays were performed at 25 • C using a UV-1800 Shimadzu (Shimadzu Co., Kyoto, Japan) spectrophotometer at 412 nm.The tested compounds were pre-incubated with the enzyme for 5 min at 25 • C prior to the addition of the substrate.The rate of substrate hydrolysis was calculated as measured by the optical density change at 412 nm change over 2 min.The sample without a substrate was used as a blank.The sample without an inhibitor was used as a control.Experiments were performed in triplicate.The percentage of AChE/BChE inhibition was determined by comparison of the rates of reaction of test samples relative to the control sample.The percentage of inhibition as a function of the compound concentration was plotted using OriginPro 8.5 software (OriginLab Corporation, Northampton, MA, USA).IC 50 (the concentration of the compound producing 50% enzyme activity inhibition) was calculated using the Hill Equation: where E is the enzyme activity and [I] is the compound concentration.