6-Chloro-3-nitro-2-[(phenylsulfonyl)methyl]imidazo[1,2-b]pyridazine

: As part of our ongoing scaffold-hopping work on an antikinetoplastid 3-nitroimidazo[1,2-a ]pyridine scaffold, we explored 3-nitroimidazo[1,2-b ]pyridazine as a potential new antikineto-plastid series. Using conditions previously described by our lab, we obtained 6-chloro-3-nitro-2-[(phenylsulfonyl)methyl]imidazo[1,2-b ]pyridazine with 54% yield. In vitro activity of this compound was evaluated against both the promastigote form of Leishmania donovani , the axenic amastigote form of Leishmania infantum and the trypomastigote blood stream form of Trypanosomabrucei brucei , and its inﬂuence on cell viability was assessed on the HepG2 cell line. However, despite good activity against the trypomastigote blood stream form of T. b. brucei (EC 50 = 0.38 µ M), it showed poor solubility in both HepG2 (CC 50 > 7.8 µ M) and L. infantum axenic amastigotes (EC 50 > 1.6 µ M) culture media, associated with a loss of activity against the promastigote form of L. infantum (EC 50 > 15.6 µ M).


Introduction
Kinetoplastids are a group of flagellated protozoa responsible for neglected tropical diseases (NTDs) in mammals, including leishmaniases (Leishmania spp.) and sleeping sickness (Trypanosoma brucei) [1]. These two diseases are found in developing countries, especially in tropical environments. More than 1 billion people are at risk of infection, and nearly 20 million people are infected, causing up to 50,000 deaths per year [2,3]. With an estimated 50,000-90,000 new cases each year, life-threatening visceral leishmaniasis (VL), caused by Leishmania infantum in the Mediterranean Basin and Latin America and L. donovani in Asia and Africa, is responsible for more than 30,000 deaths annually [4,5]. Sleeping sickness is endemic in 36 countries throughout sub-Saharan Africa and 60 million people are considered to be at risk of contracting this disease [6]. Unfortunately, in the absence of a vaccine [7], treatment of VL and sleeping sickness is based on a small number of drugs, most of them associated with significant toxicity, increasing resistance, and constraining administration [8].
The main distinguishing feature of the kinetoplastids is a subcellular structure known as a kinetoplast. Several different morphological forms of kinetoplasts have been observed and are associated with different life cycle stages in various species. Leishmania parasites 2 of 7 have two main life cycle stages: the promastigote form (found in the insect vector) and the amastigote form (inside the mammalian host). Because promastigotes are easier to obtain and maintain, they are only a preliminary evaluation for drug screening with the risk of identifying compounds that are potentially ineffective in humans. Intramacrophagic amastigotes are therefore the best model for drug evaluation but are more difficult to obtain. An alternative is to use axenic amastigotes, adapted to grow outside their host cell (macrophages) in a growth medium that mimics intracellular conditions. Trypanosomatids also have several different classes of cellular organization, two of which are adopted by T. brucei at different stages of the life cycle: epimastigotes are found in the tsetse fly vector, while trypomastigotes are found in mammalian hosts, primarily in the blood, and are referred as bloodstream forms (BSF).
With the aim of developing new antikinetoplastid molecules, our team previously identified a hit compound in 3-nitroimidazo[1,2-a]pyridine series (Hit A), bearing a phenylsulfonylmethyl substituent at position 2, a chlorine at position 6 and a 4-chlorophenylthioether moiety at position 8 (Table 1) [9]. This compound displayed low cytotoxicity (cytotoxic concentration 50% = CC 50 ) on human hepatocyte HepG2 cell line, associated with micromolar in vitro activities (measured through effective concentration 50% = EC 50 ) against both the promastigote form of L. donovani, the axenic amastigote form of L. infantum and the trypomastigote blood stream form (BSF) of T. b. brucei. of a vaccine [7], treatment of VL and sleeping sickness is based on a small number of drugs, most of them associated with significant toxicity, increasing resistance, and constraining administration [8].
The main distinguishing feature of the kinetoplastids is a subcellular structure known as a kinetoplast. Several different morphological forms of kinetoplasts have been observed and are associated with different life cycle stages in various species. Leishmania parasites have two main life cycle stages: the promastigote form (found in the insect vector) and the amastigote form (inside the mammalian host). Because promastigotes are easier to obtain and maintain, they are only a preliminary evaluation for drug screening with the risk of identifying compounds that are potentially ineffective in humans. Intramacrophagic amastigotes are therefore the best model for drug evaluation but are more difficult to obtain. An alternative is to use axenic amastigotes, adapted to grow outside their host cell (macrophages) in a growth medium that mimics intracellular conditions. Trypanosomatids also have several different classes of cellular organization, two of which are adopted by T. brucei at different stages of the life cycle: epimastigotes are found in the tsetse fly vector, while trypomastigotes are found in mammalian hosts, primarily in the blood, and are referred as bloodstream forms (BSF).
With the aim of developing new antikinetoplastid molecules, our team previously identified a hit compound in 3-nitroimidazo[1,2-a]pyridine series (Hit A), bearing a phenylsulfonylmethyl substituent at position 2, a chlorine at position 6 and a 4-chlorophenylthioether moiety at position 8 (Table 1) [9]. This compound displayed low cytotoxicity (cytotoxic concentration 50% = CC50) on human hepatocyte HepG2 cell line, associated with micromolar in vitro activities (measured through effective concentration 50% = EC50) against both the promastigote form of L. donovani, the axenic amastigote form of L. infantum and the trypomastigote blood stream form (BSF) of T. b. brucei. As part of our ongoing medicinal chemistry work to improve Hit A properties, a scaffold-hopping strategy on the 3-nitroimidazo[1,2-a]pyridine core was implemented for structure-activity relationship purposes. This scaffold-hopping approach allowed the discovery of structurally new compounds, from known active molecules, by modification of their core [10]. By replacing the carbon atom at position 5 of the 3-nitroimidazo[1,2-a]pyridine moiety with a nitrogen atom, a new 3-nitroimidazo[1,2-b]pyridazine moiety was obtained ( Figure 1). As part of our ongoing medicinal chemistry work to improve Hit A properties, a scaffold-hopping strategy on the 3-nitroimidazo[1,2-a]pyridine core was implemented for structure-activity relationship purposes. This scaffold-hopping approach allowed the discovery of structurally new compounds, from known active molecules, by modification of their core [10]. By replacing the carbon atom at position 5 of the 3-nitroimidazo[1,2a]pyridine moiety with a nitrogen atom, a new 3-nitroimidazo[1,2-b]pyridazine moiety was obtained ( Figure 1). of a vaccine [7], treatment of VL and sleeping sickness is based on a small number of drugs, most of them associated with significant toxicity, increasing resistance, and constraining administration [8].
The main distinguishing feature of the kinetoplastids is a subcellular structure known as a kinetoplast. Several different morphological forms of kinetoplasts have been observed and are associated with different life cycle stages in various species. Leishmania parasites have two main life cycle stages: the promastigote form (found in the insect vector) and the amastigote form (inside the mammalian host). Because promastigotes are easier to obtain and maintain, they are only a preliminary evaluation for drug screening with the risk of identifying compounds that are potentially ineffective in humans. Intramacrophagic amastigotes are therefore the best model for drug evaluation but are more difficult to obtain. An alternative is to use axenic amastigotes, adapted to grow outside their host cell (macrophages) in a growth medium that mimics intracellular conditions. Trypanosomatids also have several different classes of cellular organization, two of which are adopted by T. brucei at different stages of the life cycle: epimastigotes are found in the tsetse fly vector, while trypomastigotes are found in mammalian hosts, primarily in the blood, and are referred as bloodstream forms (BSF).
With the aim of developing new antikinetoplastid molecules, our team previously identified a hit compound in 3-nitroimidazo[1,2-a]pyridine series (Hit A), bearing a phenylsulfonylmethyl substituent at position 2, a chlorine at position 6 and a 4-chlorophenylthioether moiety at position 8 (Table 1) [9]. This compound displayed low cytotoxicity (cytotoxic concentration 50% = CC50) on human hepatocyte HepG2 cell line, associated with micromolar in vitro activities (measured through effective concentration 50% = EC50) against both the promastigote form of L. donovani, the axenic amastigote form of L. infantum and the trypomastigote blood stream form (BSF) of T. b. brucei.
0.95 ± 0.05 CC50 HepG2 (μM) >100 As part of our ongoing medicinal chemistry work to improve Hit A properties, a scaffold-hopping strategy on the 3-nitroimidazo[1,2-a]pyridine core was implemented for structure-activity relationship purposes. This scaffold-hopping approach allowed the discovery of structurally new compounds, from known active molecules, by modification of their core [10]. By replacing the carbon atom at position 5 of the 3-nitroimidazo[1,2-a]pyridine moiety with a nitrogen atom, a new 3-nitroimidazo[1,2-b]pyridazine moiety was obtained ( Figure 1).

Biological Results
In order to compare its biological profile to Hit A and to reference drugs (amphotericin B, miltefosine, fexinidazole and suramin), the activity of compound 3 was evaluated in vitro against the promastigote form of L. donovani, the axenic amastigote form of L. infantum and the trypomastigote blood stream form of T. b. brucei. Its influence on cell viability was assessed on the HepG2 cell line, using doxorubicin as a positive control (Table 2).

Biological Results
In order to compare its biological profile to Hit A and to reference drugs (amphotericin B, miltefosine, fexinidazole and suramin), the activity of compound 3 was evaluated in vitro against the promastigote form of L. donovani, the axenic amastigote form of L. infantum and the trypomastigote blood stream form of T. b. brucei. Its influence on cell viability was assessed on the HepG2 cell line, using doxorubicin as a positive control (Table 2).

Biological Results
In order to compare its biological profile to Hit A and to reference drugs (amphotericin B, miltefosine, fexinidazole and suramin), the activity of compound 3 was evaluated in vitro against the promastigote form of L. donovani, the axenic amastigote form of L. infantum and the trypomastigote blood stream form of T. b. brucei. Its influence on cell viability was assessed on the HepG2 cell line, using doxorubicin as a positive control (Table 2). Compound 3 displayed submicromolar in vitro activities against the trypomastigote blood stream form of T. b. brucei (EC 50 = 0.38 µM), better than Hit A (EC 50 = 0.95 µM) and the reference drug fexinidazole (EC 50 = 1.4 µM). However, it showed poor solubility in both HepG2 (CC 50 > 7.8 µM) and L. infantum axenic amastigotes (EC 50 > 1.6 µM) culture media, associated with a loss of activity against the promastigote form of L. infantum (EC 50 > 15.6 µM).
Thus, because of the lack of solubility and the loss of antileishmanial activity of compound 3, the continuation of the scaffold-hopping work on the 3-nitroimidazo[1,2b]pyridazine series has been suspended for the moment.

General
Melting points were determined on a Köfler melting point apparatus (Wagner & Munz GmbH, München, Germany) and were uncorrected. Elemental analyses were carried out at the Spectropole, Faculté des Sciences de Saint-Jérôme (Marseille) with a Thermo Finnigan EA1112 analyzer (Thermo Finnigan, San Jose, CA, USA). NMR spectra were recorded on a Bruker Avance 250MHz or a Bruker Avance NEO 400MHz NanoBay spectrometer at the Faculté de Pharmacie of Marseille. ( 1 H NMR: reference CDCl 3 δ = 7.26 ppm, reference DMSO-d 6 δ = 2.50 ppm and 13 C NMR: reference CDCl 3 δ = 76.9 ppm, reference DMSO-d 6 δ = 39.52 ppm.) The following adsorbent was used for column chromatography: silica gel 60 (Merck KGaA, Darmstadt, Germany, particle size 0.063-0.200 mm, 70-230 mesh ASTM). TLC was performed on 5 cm x 10 cm aluminum plates coated with silica gel 60F-254 (Merck) in an appropriate eluent. Visualization was performed with ultraviolet light (234 nm). Purity determination of synthesized compounds was checked by LC/MS analyses, which were realized at the Faculté de Pharmacie of Marseille with a Thermo Scientific Accela High Speed LC System ® (Waltham, MA, USA) coupled using a single quadrupole mass spectrometer Thermo MSQ Plus ® . The purity of the synthesized compound 3 was >95%. The RP-HPLC column was a Thermo Hypersil Gold ® 50 × 2.1 mm (C 18 bounded), with particles of a diameter of 1.9 mm. The volume of sample injected on the column was 1 µL. Chromatographic analysis, for a total duration of 8 min, was on the gradient of the following solvents: t = 0 min, methanol/water 50:50; 0 < t < 4 min, linear increase in the proportion of methanol to a methanol/water ratio of 95:5; 4 < t < 6 min, methanol/water 95:5; 6 < t < 7 min, linear decrease in the proportion of methanol to return to a methanol/water ratio of 50:50; 6 < t < 7 min, methanol/water 50:50. The water used was buffered with ammonium acetate 5 mM. The flow rate of the mobile phase was 0.3 mL/min. The retention times (tR) of the molecules analyzed were indicated in min. Reagents were purchased from Sigma-Aldrich or Fluorochem and used without further purification. Copies of 1 H and 13 C NMR spectra are available in the Supplementary Materials. (1) To a solution of 6-chloropyridazin-3-amine (5 g, 38.60 mmol, 1 equiv) in 1,2dimethoxyethane (80 mL), 1,3-dichloroacetone (4.90 g, 38.60 mmol, 1.1 equiv) was added. The reaction mixture was stirred and heated under reflux for 48 h. Then, the solvent was evaporated in vacuo. Compound 1 was obtained after purification by chromatography on silica gel (eluent: dichloromethane-ethyl acetate 9:1) as a white solid in 37% yield (2.9 g). mp 154 • C. 1  To a solution of 6-chloro-2-(chloromethyl)imidazo [1,2-b]pyridazine (1) (2 g, 9.90 mmol, 1 equiv) in concentrated sulfuric acid (20 mL) cooled by an ice-water bath, nitric acid 68% (2.6 mL, 59.40 mmol, 6 equiv) was added dropwise. The reaction mixture was stirred for 3 h at room temperature. Then, the mixture was slowly poured into an ice-water mixture and precipitated. The solid was collected by filtration and dried under reduced pressure.

6-Chloro-3-nitro-2-[(phenylsulfonyl)methyl]imidazo[1,2-b]pyridazine (3)
To a solution of 6-chloro-2-(chloromethyl)-3-nitroimidazo[1,2-b]pyridazine (2) (0.5 g, 2.02 mmol, 1 equiv) in dimethylsulfoxide (40 mL), sodium benzenesulfinate (0.99 g, 6.06 mmol, 3 equiv) was added. The reaction mixture was stirred for 15 h at room temperature. Then, the mixture was slowly poured into an ice-water mixture and precipitated. The solid was collected by filtration and dried under reduced pressure. Compound 3 was obtained after purification by chromatography on silica gel (eluent: dichloromethane-ethyl acetate 9:1) as a white solid in 54% yield (0.385 g). mp 253 • C. 1  The Leishmania species used in this study was L. donovani (MHOM/IN/00/DEVI) purchased from CNR Leishmania (Montpellier, France). Leishmania promastigote forms were grown in Schneider's Drosophila medium (Life Technologies, Saint-Aubin, France) supplemented with 100 U/mL penicillin, 100 µg/mL streptomycin, 2 mM L-glutamine and 20% FCS (Life Technologies, Saint-Aubin, France) at 27 • C. The in vitro evaluation of the tested compound's antileishmanial activity on promastigote forms was carried out by MTT assay according to the Mosmann protocol [12] with some modifications. Briefly, promastigotes in log-phase were incubated at an average density of 10 6 parasites/mL in sterile 96-well plates with various concentrations of compound dissolved in DMSO (final concentration less than 0.5% v/v), in duplicate. Appropriate controls treated with DMSO, Miltefosine, Amphotericin B and Fexinidazole (reference drugs purchased from Sigma-Aldrich, Saint-Louis, MI, USA) were added to each set of experiments. After a 72h incubation period at 27 • C, parasitic metabolic activity was determined. Each plate-well was then microscope-analyzed to detect any precipitate formation. A total of 20 µL of MTT (3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma-Aldrich, Saint-Louis, MI, USA) solution (5 mg/mL in PBS) was added to each well and incubated for 4 h at 27 • C. The enzyme reaction was then stopped by addition of 100 µL of 50% isopropanol−10% sodium dodecyl sulfate. Plates were shaken vigorously at 300 rpm for 10 min. The absorbance was measured at 570 nm in a BIO-TEK Elx808 (Biotek, Colmar, France) absorbance microplate reader. An inhibitory concentration of 50% (EC 50 ) was defined as the concentration of drug required to inhibit the metabolic activity of Leishmanial promastigote forms by 50% relative to the control. EC 50 values were calculated by non-linear regression analysis processed dose-response curves, using TableCurve 2D V5.0 software. EC 50 values represent the mean value calculated from at least three separate experiments.

Antileishmanial Activity on L. infantum Axenic Amastigotes
L. infantum promastigotes were harvested in the logarithmic phase of growth by centrifugation at 900 g for 10 min. The supernatant was removed carefully and was replaced by the same volume of RPMI 1640 complete medium at pH 5.4 and incubated for 24 h at 24 • C. The acidified promastigotes were then incubated for 24 h at 37 • C in a ventilated flask to transform them into axenic amastigotes. The amastigote stage was checked both by electron microscopy (short flagellum with small bulbous tip extending beyond a spherical cell body) and RT-PCR to confirm the overexpression of ATG8 and amastin genes in amastigotes, relative to promastigotes. The effects of the tested compounds on the growth of L. infantum axenic amastigotes were assessed as follows. L. infantum amastigotes were incubated at a density of 2 × 10 6 parasites/mL in sterile 96-well plates with various concentrations of a compound dissolved in DMSO (final concentration less than 0.5% v/v), in duplicate. Appropriate controls treated with DMSO, Amphotericin B, Miltefosine, and