4,6-Bis((E)-2-(4 - N , N -diphenylamino-[1,1 (cid:48) -biphenyl]-4-yl)vinyl)-2-phenylpyrimidine

: In this contribution, we designed a 4,6-distyrylpyrimidine chromophore with diphenylamino electron-donating groups and biphenylenevinylene extended π -conjugated linkers. This compound has been synthesized in two steps from 4,6-dimethyl-2-phenylpyrimidine by a double Knoevenagel reaction with 4-bromobenzaldehyde followed by a double Suzuki–Miyaura cross coupling reaction with 4-( N , N -diphenylamino)phenylboronic acid. This compound exhibits intense emission in moderately polar solvents as well as in solid state. This compound is characterized by an intense emission solvatochromism with emission ranging from blue in non polar n -heptane to orange in dichloromethane. This chromophore is also sensible to the presence of acid with a bathochromic shift of the charge transfer absorption band and emission quenching.


Introduction
During the past two decades, there has been an important interest in the design of pyrimidine-based emissive materials [1,2]. The pyrimidine heterocycle is a six-membered aromatic ring with two nitrogen atoms in positions 1 and 3. Due to its π-deficient character, this heterocycle can be used as the electron-withdrawing part in push-pull structures [2] and the electron-lone pairs of the two nitrogen atoms are available for protonation, complexation and formation of hydrogen bonds, enabling us to tune the electron-withdrawing strength of this core [2,3]. Pyrimidine chromophores have been extensively used as fluorescent sensors for acidity [4], polarity [5], metal cations [6] or nitro-aromatic explosives [7]. Pyrimidine derivatives have also been used with success as emitters for organic light emitting diodes (OLEDs), in particular for third generation OLEDs based on thermally activated delayed fluorescence [8,9]. White light emission has also been described by controlled protonation of some pyrimidine compounds [4,10]. Finally, 4,6-distyrylpyrimidine derivatives are also well-known for their two-photon absorption properties and numerous structures have been developed for bio-imaging in particular [11]. In this short note we describe a new 4,6-distyrylpyrimidine emissive material combining diphenylamino strong electrondonating groups that is known to induce strong emission [12] and biphenylenevinylene extended π-conjugated linkers that should induce strong emission solvatochromism [13]. The presence of a phenyl ring in position 2 of the pyrimidine core may influence the photophysical and protonation properties of the chromophores.

Synthesis
The title compound was obtained in two steps from 4,6-dimethyl-2-phenylpyrimidine [14] (Scheme 1). The first step consists of a double Knoevenagel condensation with p-bromobenzaldehyde in a boiling 5 M aqueous solution of sodium hydroxyde in the presence of aliquat 336 as a phase transfer catalyst according to conditions initially described by Vanden p-bromobenzaldehyde in a boiling 5 M aqueous solution of sodium hydroxyde in the presence of aliquat 336 as a phase transfer catalyst according to conditions initially described by Vanden Eynde [15]. The second step is a double Suzuki-Miyaura palladium catalyzed cross coupling reaction with p-(N,N-diphenylamino)phenylboronic acid leading to title compound 1 with a yield of 68%. The structure of compound 1 is confirmed by 1 H and 13 C-NMR as well as HRMS analysis. The E-stereochemistry of the double bond is confirmed by the coupling constant of ~16 Hz observed for the doublet at 8.16 ppm, corresponding to the vinylene linker (the second doublet is hidden in the massif around 7.20-7.10 ppm). Scheme 1. Synthesis of titled chromophore.

Photophysical Properties
The photophysical properties of the title compound were measured in a series of aprotic solvents of increasing polarity and the results are summarized in Table 1. Whatever the solvents, two absorption bands are observed, with the less energetic one around 400 nm being attributed to charge transfer. The normalized absorption and emission spectra in dichloromethane (DCM) are presented in Figure 1. In DCM, when compared with analogue pyrimidine chromophore bearing phenylenevinylene linkers instead of biphenylenevinylene bridges [16], a blue shift of 23 nm of the less energetic absorption band and a red shift of 53 nm of the emission band is observed. The analogue chromophore without the phenyl ring in the C2 position exhibits similar emission properties in DCM (λem = 485 nm) [13].

Photophysical Properties
The photophysical properties of the title compound were measured in a series of aprotic solvents of increasing polarity and the results are summarized in Table 1. Whatever the solvents, two absorption bands are observed, with the less energetic one around 400 nm being attributed to charge transfer. The normalized absorption and emission spectra in dichloromethane (DCM) are presented in Figure 1. In DCM, when compared with analogue pyrimidine chromophore bearing phenylenevinylene linkers instead of biphenylenevinylene bridges [16], a blue shift of 23 nm of the less energetic absorption band and a red shift of 53 nm of the emission band is observed. The analogue chromophore without the phenyl ring in the C2 position exhibits similar emission properties in DCM (λ em = 485 nm) [13]. a Reichardt polarity parameter [17]. b calculated using the less energetic absorption band. a Reichardt polarity parameter [17]. b calculated using the less energetic absorption band. Whereas the position of the absorption spectrum is almost not sensible to the polarity of the solvent, a remarkable positive emission solvatochromism is noticed ( Figure 2): a significant red shift of the emission band is observed when the polarity is increased, which is characteristic of luminescent push-pull materials because of the stabilization of a highly polar excited state in polar solvents [18]. In the most polar solvents tested, acetone and MeCN, the emission intensity is significantly reduced. Compound 1 exhibits one of the largest solvatochromic ranges observed for pyrimidine chromophore so far. Photoluminescence quantum yields of 0.87 and 0.83 have been measured in toluene and DCM, respectively. The effect of aggregation has also been examined by a progressive increase in the water ratio in the THF/water mixture ( Figure 3). The addition of water up to 50% induces a dramatic decrease in emission intensity with an emission band that is red-shifted due to an increase in polarity. Then, when the water ratio increases up to 70%, an increase of emission intensity is observed with a slightly red-shifted band with regard to pure THF solution, which is attributed to the formation of aggregates. When the ratio of water is further increased, a slight decrease in emission intensity is observed and attributed to Whereas the position of the absorption spectrum is almost not sensible to the polarity of the solvent, a remarkable positive emission solvatochromism is noticed ( Figure 2): a significant red shift of the emission band is observed when the polarity is increased, which is characteristic of luminescent push-pull materials because of the stabilization of a highly polar excited state in polar solvents [18]. In the most polar solvents tested, acetone and MeCN, the emission intensity is significantly reduced. Compound 1 exhibits one of the largest solvatochromic ranges observed for pyrimidine chromophore so far. Photoluminescence quantum yields of 0.87 and 0.83 have been measured in toluene and DCM, respectively.
Molbank 2021, 2021, x FOR PEER REVIEW 3 of 7 a Reichardt polarity parameter [17]. b calculated using the less energetic absorption band. Whereas the position of the absorption spectrum is almost not sensible to the polarity of the solvent, a remarkable positive emission solvatochromism is noticed ( Figure 2): a significant red shift of the emission band is observed when the polarity is increased, which is characteristic of luminescent push-pull materials because of the stabilization of a highly polar excited state in polar solvents [18]. In the most polar solvents tested, acetone and MeCN, the emission intensity is significantly reduced. Compound 1 exhibits one of the largest solvatochromic ranges observed for pyrimidine chromophore so far. Photoluminescence quantum yields of 0.87 and 0.83 have been measured in toluene and DCM, respectively. The effect of aggregation has also been examined by a progressive increase in the water ratio in the THF/water mixture (Figure 3). The addition of water up to 50% induces a dramatic decrease in emission intensity with an emission band that is red-shifted due to an increase in polarity. Then, when the water ratio increases up to 70%, an increase of emission intensity is observed with a slightly red-shifted band with regard to pure THF solution, which is attributed to the formation of aggregates. When the ratio of water is further increased, a slight decrease in emission intensity is observed and attributed to The effect of aggregation has also been examined by a progressive increase in the water ratio in the THF/water mixture (Figure 3). The addition of water up to 50% induces a dramatic decrease in emission intensity with an emission band that is red-shifted due to an increase in polarity. Then, when the water ratio increases up to 70%, an increase of emission intensity is observed with a slightly red-shifted band with regard to pure THF solution, which is attributed to the formation of aggregates. When the ratio of water is further increased, a slight decrease in emission intensity is observed and attributed to precipitation with a blue shift of the emission band. Solid state emissions in potassium bromide matrix were also tested ( Figure 4) and an intense yellow emission at 545 nm was observed.  Finally, the effect of protonation of the photophysical properties was tested. Figure 5 presents the effect of the progressive addition of trifluoroacetic acid (TFA) on the absorption and emission spectra of chromophore 1 in DCM. The addition of TFA leads to a decrease in the absorption band of the neutral form centered at 403 nm and the progressive appearance of a new red-shifted band centered at 544 nm, attributed to the protonated form of 1. The protonation of the pyrimidine reinforces its electron-withdrawing strength and induces an increased intramolecular charge transfer into the chromophore, resulting in a red shifted absorption. The pyrimidine ring is considered a monobase (pKa~1.1). The protonation of the second nitrogen atom is not observed (pKa~−6.3). An isobestic point is observed at 438 nm. It should be noted that as already observed with push-pull chromophores with extended π-conjugated linkers [19], a large amount of acid is required to observe the formation of the protonation form. The protonation of 1 provokes an extinction of emission in DCM as generally observed for amino substituted pyrimidine chromophores [4].
However, in n-heptane, a low intensity emission centered at 706 nm is observed after the addition of a few drops of TFA with excitation at 530 nm ( Figure S1). Emission quenching is observed in solvents of higher polarity after the addition of TFA.

General Informations
All solvents were reagent grade for synthesis and spectroscopic grade for photophysical measurements. The starting materials were purchased from Sigma-Aldrich or TCI (Sigma-Aldrich, Saint Louis, MO,USA or Tokyo Chemical Industry, Tokyo, Japan) and were used without further purification. NMR spectra were recorded in CDCl3 on a Bruker Avance 300 ( 1 H at 300 MHz and 13 C at 75 MHz) spectrometer (Bruker BioSpin GmbH, Rheinstetten, Germany). The chemical shifts δ are reported in ppm and are referenced to the residual protons of the deuterated solvent or carbon nuclei ( 1 H, δ = 7.27 ppm; 13 C, δ = 77.0 ppm). The coupling constants J are given in Hz. In the 1 H-NMR spectra, the following abbreviations are used to describe the peak patterns: s (singlet), d (doublet), dd (doublet of doublets), t (triplet), and m (multiplet). Acidic impurities in CDCl3 were removed by treatment with solid K2CO3. Melting points (°C) were measured on a Kofler hot stage with a precision of 2 degrees (±2 °C). High-resolution mass analyses were performed at the 'Centre Régional de Mesures Physiques de l'Ouest' (CRMPO, Université de Rennes 1) using a Bruker MicroTOF-Q II apparatus. Analytical thin layer chromatography (TLC) was performed on silica gel plates (Merck 60 F254) and compounds were visualized by irradiation with UV light at 254 and 365 nm. Flash chromatography was performed using silica Acros SI 60 (60-200 mesh ASTM) (Acros, Geel, Belgium). UV-visible and fluorescence spectroscopy studies in solution were conducted on a Spex Fluoromax-3 Jobin-Yvon Horiba spectrophotometer (Horiba, Kyoto, Japan). Compounds were excited at their absorption maxima (band of lowest energy) to record the emission spectra. All solutions were measured with optical densities below 0.1. Fluorescence quantum yields (±10%) were determined relative to 9,10-bis(phenylethynyl)anthracene in cyclohexane (ФF = 1.00) [20]. Acidic impurities in solvents were removed by treatment with Al2O3. Stokes shifts were calculated considering the lowest energetic absorption band.

General Informations
All solvents were reagent grade for synthesis and spectroscopic grade for photophysical measurements. The starting materials were purchased from Sigma-Aldrich or TCI (Sigma-Aldrich, Saint Louis, MO, USA or Tokyo Chemical Industry, Tokyo, Japan) and were used without further purification. NMR spectra were recorded in CDCl 3 on a Bruker Avance 300 ( 1 H at 300 MHz and 13 C at 75 MHz) spectrometer (Bruker BioSpin GmbH, Rheinstetten, Germany). The chemical shifts δ are reported in ppm and are referenced to the residual protons of the deuterated solvent or carbon nuclei ( 1 H, δ = 7.27 ppm; 13 C, δ = 77.0 ppm). The coupling constants J are given in Hz. In the 1 H-NMR spectra, the following abbreviations are used to describe the peak patterns: s (singlet), d (doublet), dd (doublet of doublets), t (triplet), and m (multiplet). Acidic impurities in CDCl 3 were removed by treatment with solid K 2 CO 3 . Melting points ( • C) were measured on a Kofler hot stage with a precision of 2 degrees (±2 • C). High-resolution mass analyses were performed at the 'Centre Régional de Mesures Physiques de l'Ouest' (CRMPO, Université de Rennes 1) using a Bruker MicroTOF-Q II apparatus. Analytical thin layer chromatography (TLC) was performed on silica gel plates (Merck 60 F254) and compounds were visualized by irradiation with UV light at 254 and 365 nm. Flash chromatography was performed using silica Acros SI 60 (60-200 mesh ASTM) (Acros, Geel, Belgium). UV-visible and fluorescence spectroscopy studies in solution were conducted on a Spex Fluoromax-3 Jobin-Yvon Horiba spectrophotometer (Horiba, Kyoto, Japan). Compounds were excited at their absorption maxima (band of lowest energy) to record the emission spectra. All solutions were measured with optical densities below 0.1. Fluorescence quantum yields (±10%) were determined relative to 9,10-bis(phenylethynyl)anthracene in cyclohexane (Φ F = 1.00) [20]. Acidic impurities in solvents were removed by treatment with Al 2 O 3 . Stokes shifts were calculated considering the lowest energetic absorption band.