5-((8-Hydroxyquinolin-5-yl)diazenyl)-3-methyl-1 H -pyrazole-4-carboxylic Acid

: A new azo compound was prepared via the azo coupling reaction between 4-(ethoxycarbonyl)-3-methyl-1 H -pyrazole-5-diazonium chloride and 8-hydroxyquinoline (oxine). The ester functional group of the obtained compound was hydrolyzed and thus a new chemical structure with a carboxylic functional group resulted. The structures of the new compounds were fully characterized by: UV–Vis, FT-IR, 1D and 2D NMR spectroscopy, and HRMS spectrometry.


Introduction
Azo compounds contain the chromophoric azo (diazene, according to IUPAC nomenclature) functional group (-N=N-). The substituents attached to the diazene group can be aromatic or heterocyclic. An extended conjugation occurs, thus giving the compounds the property either to absorb or to emit light [1]. Azo colorants form the biggest and most well-known group of colorants, and account for approximately 60-70% of them [2].
8-Hydroxyquinoline and its derivatives belong to the most well-known ligands for metal complexes used for example in Organic Light Emitting Diodes (OLEDs) [9], chemosensors for metal ions, antibacterial, anti-HIV agents, and gravimetric metal analysis [10].
Given the above, we decided to synthesize azo compounds which would carry two important moieties: pyrazole and 8-hydroxyquinoline. The properties of the new compounds such as dyeing capability, maximum emission wavelength, metal chelating capability, and thermal stability are subject of further studies.
The azo coupling reaction was carried out in alkaline medium because in this medium, 8-hydroxyquinoline exists as the 8-quinolinate anion which has a higher electron density in the positions 2, 4, 5, and 7, as seen in the below resonance structures (Scheme 2). The so-called pyridine ring of the 8-quinolinate anion is not very active towards electrophilic substitution reactions [16], the azo coupling reaction occurs only in the C-5 and C-7 positions due to increased electron density in these positions. However, the experimental NMR data from our study (available in the Supplementary Materials Section) shows that coupling took place regioselectively in the C-5 position. From the literature [17] we found that at a molar ratio of 1:1 (diazonium salt:8-hydroxyquinoline) the coupling took place only in the C-5 position, but on increasing the diazonium salt ratio the C-5 and C-7 disubstituted compound could be obtained. If the C-5 position is occupied, e.g., as in the case of 5-chloro-8-hydroxyquinoline or 8-hydroxyquinoline-5-sulfonic acid the coupling takes place in the C-7 position [18].

Experimental NMR Data for Compound (3) Explanation
From the long-range coupling spectrum 1 H-15 N HMBC it can be seen that the nitrogen atom, whose signal is found at 298.8 ppm couples with the proton whose signal is found at 8.97 ppm, with the proton at 7.52 ppm, and even with the proton at 7.47 ppm.
From the 1 H-13 C COSY spectrum it can be noted that the proton at 7.47 ppm couples with the protons at 9.45 ppm and 8.97 ppm, also the proton at 7.52 ppm couples with the proton at 8.56 ppm. We can conclude that the proton at 7.47 ppm is the 3-H proton, because it couples with two other protons, and the protons with signals at 8.56 ppm and 7.52 ppm are the 6-H and 7-H protons.
Due to the fact that the coupling between the nitrogen atom 1-N and the proton from 7.52 ppm takes place and this coupling is a long-range coupling (over four bonds), we can conclude that the signal at 7.52 ppm corresponds to the 7-H proton, thus the signal from the 8.56 ppm corresponds to the 6-H proton.
From the coupling of the 1-N nitrogen atom and the proton at 8.97 ppm we can conclude that this signal corresponds to the 2-H proton, and the remaining signal at 9.45 ppm can be attributed to the 4-H proton. Using the long-range coupling information we can conclude that the 7-H position remained unoccupied and the azo-coupling took place only in the position 5 of 8-hydroxyquinoline.
The assignment of the signals of the carbon atoms was made easier by the 1 H-13 C HSQC direct coupling spectrum between carbon atoms and the proton directly bonded to them.
From the 2D NOESY spectrum through-space correlations can be observed between the 3-H proton (at 7.47 ppm) and 4-H (at 9.45 ppm) and 2-H (8.97 ppm). In addition through-space correlation between the 7-H proton (at 7.52 ppm) and the 6-H proton (at 8.49 ppm can be observed).
From the HMBC spectrum the coupling can be observed between the carbon atom from 140.8 ppm (8a-C) and the 7-H proton from 7.52 ppm).
In the table below (Table 1) correlations are presented between direct bonding protons to the carbon atoms ( 1 H-13 C HSQC experiment), long-range correlations between protons, and carbon atoms ( 1 H-13 C HMBC experiment) long-range correlation between protons and nitrogen atom ( 1 H-15 N HMBC), long-range correlations between protons ( 1 H-1 H COSY) and couplings through-space between protons (2D-NOESY):   In conclusion, it was shown (mainly by 1D and 2D NMR data) that the azo coupling reaction between the diazonium salt (2) and 8-hydroxyquinoline takes place regioselectively in the C-5 position of 8-hydroxyquinoline. The hydrolysis of the azo compound (3) yielded the corresponding carboxylic acid (4) (confirmed by NMR and IR data). Both compounds (3) and (4) were obtained in a high yield and purity and can be used in further studies.

Materials and Methods
The reagents were purchased from commercial sources and were used as received. The 5-amino-3-methyl-1H-pyrazole-4-carboxylate was synthesized earlier in our laboratory following a modified procedures from the literature [19].
The 1D and 2D NMR spectra were recorded on a Bruker Avance III 500 MHz spectrometer. Chemical shifts (δ) were measured in ppm and the coupling constants (J) in Hz. Samples were dissolved in Py-d5 or DMSO-d6.
IR spectra were recorded on a Bruker Vertex 70 (Bruker Optics GmbH, Ettlingen, Germany) equipped with a Platinum ATR spectrometer, Bruker Diamond Type A225/Q.I.
UV-Vis spectra were recorded on a Jasco V-530 spectrometer and the samples were dissolved in 96% ethanol. The concentration for compound (3) solution was 5.56·10 −5 M and the concentration for compound (4) solution was 6.73·10 −5 M.
Melting points were measured on a Böetius PHMK (Veb Analytik, Dresden, Germany) apparatus and were uncorrected.
High resolution MS (HRMS) spectra were recorded on a Bruker Maxis II QTOF spectrometer (Bruker Daltonics, Bremen, Germany) with electrospray ionization (ESI) either in positive or negative mode. The compounds were initially dissolved in DMSO and further diluted 1:100 with acetonitrile. MS spectra processing and isotope pattern simulations were performed with Compass Data Analysis V.4.4 (Bruker Daltonics, Bremen, Germany).
After cooling down the 8-hydroxyquinoline solution to a temperature below 5 • C, the solution of 4-(ethoxycarbonyl)-3-methyl-1H-pyrazole-5-diazonium chloride (2), which was obtained by diazotization of 0.444 g (2.625 mmol) of ethyl 5-amino-3-methyl-1H-pyrazole-4carboxylate (1) using 0.192 g (2.756 mmol) NaNO 2 and 0.68 mL (8.125 mmol) concentrated HCl, was added dropwise. An intense red coloring of the reaction mass was observed. After addition of the diazonium chloride solution, the pH of the reaction mass was checked and adjusted to pH 7-8. Besides the pH checking, the rate of the azo compound formation was monitored by reacting a few drops of reaction mass with an alkaline H acid (4-amino-5-hydroxynaphthalene-2,7-disulfonic acid) solution on a filter paper. By this method it was determined whether the diazonium salt was present or not (if present a dark violet spot appears on the filter paper). The reaction mass was left stirring for about 5 h.
After completion, the reaction mass was acidified down to a pH of 5-6 and then was precipitated in distilled water. A dark red precipitate was formed. The precipitate was vacuum filtered and dried. The final product (3) had an appearance of a dark brown-red solid.
An amount of 728 mg (2.221 mmol) of compound (

Synthesis of 5-((8-Hydroxyquinolin-5-Yl)Diazenyl)-3-Methyl-1h-Pyrazole-4-Carboxylic Acid (4)
An amount of 350 mg (1.078 mmol) of compound (3) and 15 mL of 10% NaOH (aq.) were added to a round bottom flask equipped with a condenser column and magnetic stirrer. Then the reaction mass was refluxed for about 10 h to ensure full conversion of ester to carboxylic acid (reaction monitoring by means of TLC was not possible due to the low solubility of the compounds in the usual organic solvents).
After 10 h the reaction mass was cooled down and acidified to pH 5-6. The precipitate was vacuum filtered and allowed to dry.